##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938718_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1320639 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94628509380686 31.0 31.0 34.0 30.0 34.0 2 32.09859015219148 33.0 31.0 34.0 30.0 34.0 3 32.16054652331182 34.0 31.0 34.0 30.0 34.0 4 35.78367896147244 37.0 35.0 37.0 35.0 37.0 5 35.71594054090482 37.0 35.0 37.0 33.0 37.0 6 35.80367155596647 37.0 35.0 37.0 33.0 37.0 7 35.73201079174551 37.0 35.0 37.0 33.0 37.0 8 35.754794459348844 37.0 35.0 37.0 33.0 37.0 9 37.32288081754363 39.0 37.0 39.0 34.0 39.0 10-11 37.239245547042 39.0 37.0 39.0 33.0 39.0 12-13 37.15193705471366 39.0 37.0 39.0 33.0 39.0 14-15 38.12209657597572 40.0 37.0 41.0 33.0 41.0 16-17 38.086979484931156 40.0 37.0 41.0 33.0 41.0 18-19 38.39618245409987 40.0 38.0 41.0 33.5 41.0 20-21 38.42171214086514 40.0 38.0 41.0 34.0 41.0 22-23 38.3330842115067 40.0 38.0 41.0 33.5 41.0 24-25 38.20805799313817 40.0 37.0 41.0 33.0 41.0 26-27 37.88174966815307 40.0 36.5 41.0 33.0 41.0 28-29 37.650989028795905 39.5 36.5 41.0 32.5 41.0 30-31 37.39102396642838 39.0 36.0 41.0 32.0 41.0 32-33 37.12037089621009 39.0 35.0 41.0 32.0 41.0 34-35 37.016869863755346 39.0 35.0 41.0 31.0 41.0 36-37 36.7794700141371 39.0 35.0 41.0 31.0 41.0 38-39 36.543480088048284 39.0 35.0 41.0 30.5 41.0 40-41 36.30618170446276 39.0 35.0 41.0 30.0 41.0 42-43 36.121575237441874 39.0 35.0 41.0 30.0 41.0 44-45 35.819401062667396 38.5 35.0 41.0 29.5 41.0 46-47 35.341306367599316 38.0 35.0 40.0 26.5 41.0 48-49 34.930822124744154 38.0 34.5 40.0 24.5 41.0 50-51 34.69339312257172 38.0 34.0 40.0 24.0 41.0 52-53 34.376654028845124 38.0 34.0 40.0 23.0 41.0 54-55 34.15272303786273 38.0 34.0 40.0 23.0 41.0 56-57 33.96128692246708 38.0 33.0 40.0 23.0 41.0 58-59 33.72277889718538 37.0 33.0 40.0 22.0 41.0 60-61 33.276464272219734 36.5 33.0 40.0 21.0 41.0 62-63 32.84123329691157 36.0 32.0 39.0 20.0 41.0 64-65 32.323094350537886 35.5 31.5 39.0 18.0 40.0 66-67 31.887139104630407 35.0 31.0 38.5 14.5 40.0 68-69 31.618337032300275 35.0 31.0 38.0 13.0 40.0 70-71 31.1428933266396 35.0 31.0 37.0 8.0 39.0 72-73 30.576773062131288 35.0 30.5 36.5 6.5 39.0 74-75 30.077499225753595 34.0 29.5 36.0 2.0 38.5 76-77 28.075406299526215 32.0 26.5 34.5 2.0 36.0 78-79 29.011102201282863 34.0 29.0 35.0 2.0 37.0 80-81 29.118086017450644 34.0 29.0 35.0 2.0 36.5 82-83 28.814061980601814 34.0 29.0 35.0 2.0 36.0 84-85 28.4482932126039 34.0 29.0 35.0 2.0 36.0 86-87 27.82090601595137 33.0 27.0 35.0 2.0 35.0 88-89 27.094127539774306 33.0 25.5 35.0 2.0 35.0 90-91 26.465994113455682 33.0 24.5 35.0 2.0 35.0 92-93 26.214211832302393 32.5 24.0 35.0 2.0 35.0 94-95 25.892879886176313 32.5 24.0 35.0 2.0 35.0 96-97 25.14129826546089 32.0 19.5 34.0 2.0 35.0 98-99 24.264918346345972 32.0 4.5 34.0 2.0 35.0 100-101 20.635736185286063 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.03050650907359298 1101 2 0.08703664624228225 1101 3 -0.034456093315888836 1101 4 0.021065878564037632 1101 5 -0.06313757899123829 1101 6 0.10724939668904199 1101 7 0.17607023899866192 1101 8 0.17285800751050573 1101 9 0.231633780407563 1101 10-11 0.26523027962281986 1101 12-13 0.196554680439867 1101 14-15 0.179548912368503 1101 16-17 0.4185501106238121 1101 18-19 0.32587874530796057 1101 20-21 0.20407801069146814 1101 22-23 0.10498793282058472 1101 24-25 0.13881533573346871 1101 26-27 -0.018018375376350093 1101 28-29 -0.05326842072564375 1101 30-31 0.12587922357047887 1101 32-33 0.3227298784005441 1101 34-35 0.36739034939751747 1101 36-37 0.3209443440043884 1101 38-39 0.6827395643532839 1101 40-41 0.5763234817072558 1101 42-43 0.6260094099498374 1101 44-45 0.698950721244394 1101 46-47 0.7411114134504615 1101 48-49 0.6016359568476801 1101 50-51 0.6720177308581867 1101 52-53 0.538255352856936 1101 54-55 0.764372872494782 1101 56-57 0.6554769922232779 1101 58-59 0.6905678464632174 1101 60-61 0.588173855441319 1101 62-63 0.5506145759069696 1101 64-65 0.44449910112360413 1101 66-67 0.5342065153818503 1101 68-69 0.4957189136514124 1101 70-71 0.619228195410205 1101 72-73 0.4617889504381374 1101 74-75 0.3864510349810928 1101 76-77 0.3588511099173921 1101 78-79 0.3559887974236311 1101 80-81 0.39392950714610464 1101 82-83 0.45240502905849667 1101 84-85 0.4765009315818709 1101 86-87 0.45320836767570327 1101 88-89 0.6043573712914068 1101 90-91 0.5114694379531137 1101 92-93 0.6674115600884853 1101 94-95 0.5728580878628335 1101 96-97 0.40196686734610765 1101 98-99 0.22594976804351674 1101 100-101 0.7180682195364447 1102 1 0.2762462061081443 1102 2 0.17298585077963224 1102 3 0.16732626619994306 1102 4 0.17634399323431893 1102 5 0.08079003271835461 1102 6 0.07065811467434457 1102 7 0.1096615923553017 1102 8 0.13206349608390155 1102 9 0.2874790635161659 1102 10-11 0.2419198946141421 1102 12-13 0.23509894811483179 1102 14-15 0.3544627075220106 1102 16-17 0.31768152248083936 1102 18-19 0.2870170059914159 1102 20-21 0.32801323220819967 1102 22-23 0.24776099410330943 1102 24-25 0.15240835373542438 1102 26-27 0.3206525242455385 1102 28-29 0.381639785975743 1102 30-31 0.3046918558503293 1102 32-33 0.3336737322922474 1102 34-35 0.2858274852563554 1102 36-37 0.34081426386360647 1102 38-39 0.43604507432926454 1102 40-41 0.5133208182747637 1102 42-43 0.4720380418491956 1102 44-45 0.5416322270349809 1102 46-47 0.5740666344915724 1102 48-49 0.40642300115102614 1102 50-51 0.15058423343839422 1102 52-53 0.1879670125083095 1102 54-55 0.08162929921471829 1102 56-57 0.12871544077384556 1102 58-59 0.3961206276099247 1102 60-61 0.4534404364976581 1102 62-63 0.37749978672240303 1102 64-65 0.43151427317658886 1102 66-67 0.5537577745671207 1102 68-69 0.4108850214728683 1102 70-71 0.5560810645453067 1102 72-73 0.496808926181874 1102 74-75 0.5693390756935628 1102 76-77 0.4633527864530258 1102 78-79 0.4569970968695465 1102 80-81 0.6696518443081239 1102 82-83 0.6454188693952325 1102 84-85 0.6946396203169769 1102 86-87 0.6569460909165308 1102 88-89 0.8507123925750903 1102 90-91 0.8043721037636757 1102 92-93 0.9734619512801324 1102 94-95 1.0743479454167577 1102 96-97 1.2318395219797686 1102 98-99 1.2535079711778074 1102 100-101 1.8754770802056306 1103 1 0.14615069964673566 1103 2 0.12720164687853242 1103 3 0.09764927898417852 1103 4 0.04258759822790381 1103 5 -0.09680694618246122 1103 6 -0.003931623289183506 1103 7 -0.05696311460038572 1103 8 -0.01301278205123424 1103 9 0.015527013072123452 1103 10-11 0.10654323884159567 1103 12-13 0.09565085304721777 1103 14-15 0.1396678674116103 1103 16-17 0.0589190644886699 1103 18-19 0.06501125481634773 1103 20-21 0.13802120070376844 1103 22-23 0.21581194628078748 1103 24-25 0.07267604899243452 1103 26-27 0.1692863314541384 1103 28-29 -0.04213346990535882 1103 30-31 0.251193224352825 1103 32-33 0.3790485402976955 1103 34-35 0.1827284333918371 1103 36-37 0.3045974514928105 1103 38-39 0.31565438707539073 1103 40-41 0.3012369989401904 1103 42-43 0.36557374417391486 1103 44-45 0.412749317725698 1103 46-47 0.39700897260985357 1103 48-49 0.4790448671648164 1103 50-51 0.5003093780839123 1103 52-53 0.3754963169394827 1103 54-55 0.36049835027082366 1103 56-57 0.14982733461962283 1103 58-59 0.2995349547339927 1103 60-61 0.533593685680593 1103 62-63 0.21869748625238117 1103 64-65 0.2515983465791436 1103 66-67 0.4209824283995083 1103 68-69 0.38993422982015247 1103 70-71 0.3190575863547309 1103 72-73 0.4870215348825475 1103 74-75 0.5801600617082734 1103 76-77 0.45305841405866687 1103 78-79 0.18454672673869865 1103 80-81 0.4196905319315505 1103 82-83 0.4034474173967588 1103 84-85 0.27648184410466925 1103 86-87 0.3211259710426404 1103 88-89 0.24300373925083818 1103 90-91 0.1251577512328126 1103 92-93 0.19939074295594494 1103 94-95 0.3972839318356698 1103 96-97 0.3777628858397506 1103 98-99 0.769178190946608 1103 100-101 1.404464503840881 1104 1 0.02665378114924266 1104 2 0.07452044962220583 1104 3 -0.031616515668602574 1104 4 -0.056939800591258916 1104 5 -0.1186954319397131 1104 6 0.045833191798351436 1104 7 -0.03738742923941629 1104 8 -0.09976901534395921 1104 9 0.08111548856514617 1104 10-11 0.029252417300980937 1104 12-13 -0.04188713578707848 1104 14-15 -0.08126147000957218 1104 16-17 0.1650350994584855 1104 18-19 0.12178754799866454 1104 20-21 0.0037878499658532405 1104 22-23 -0.028227544256033354 1104 24-25 0.01753366617208485 1104 26-27 0.02567459184445653 1104 28-29 -0.08538941981587556 1104 30-31 -0.19600620084988662 1104 32-33 -0.11259391615738679 1104 34-35 -0.13456702936905884 1104 36-37 -0.03771430016620769 1104 38-39 -0.16032897147226066 1104 40-41 -0.13664484183917835 1104 42-43 -0.19298819157195624 1104 44-45 -0.19900368010096514 1104 46-47 -0.10472088605605734 1104 48-49 0.02197615479105508 1104 50-51 -0.2074242783497624 1104 52-53 -0.15071095817331326 1104 54-55 0.017936128911394178 1104 56-57 -0.19295021258471223 1104 58-59 -0.13415026342567415 1104 60-61 0.010795364552784292 1104 62-63 0.025847531548912173 1104 64-65 -0.0938696580085292 1104 66-67 -0.045267197848378515 1104 68-69 -0.01408176453897525 1104 70-71 0.004466526951571126 1104 72-73 0.01959709975029611 1104 74-75 0.08372242141177111 1104 76-77 -0.001127386484892412 1104 78-79 0.00878840043413831 1104 80-81 0.1809198305271309 1104 82-83 0.11507423204694689 1104 84-85 0.25885971083916814 1104 86-87 0.4804531502963876 1104 88-89 0.42064979265164837 1104 90-91 0.24971482708552628 1104 92-93 0.29875581063296863 1104 94-95 0.25695507460228484 1104 96-97 0.385163735540079 1104 98-99 0.620699306267543 1104 100-101 1.1640576607185942 1105 1 0.048571274533269815 1105 2 0.02205792524464556 1105 3 0.04469920334121724 1105 4 0.028215434839104603 1105 5 0.09230046682813509 1105 6 -0.012252349461647327 1105 7 -0.045616669819750655 1105 8 -0.026628515787997742 1105 9 -0.014730173009567693 1105 10-11 0.005938095119965681 1105 12-13 -0.004954290674419326 1105 14-15 0.1124363999380833 1105 16-17 0.12359379973829476 1105 18-19 0.002984023348872711 1105 20-21 0.006402441248390289 1105 22-23 -0.0024180083334357505 1105 24-25 -0.1039502747594554 1105 26-27 0.01875683069771128 1105 28-29 0.012329465041695187 1105 30-31 -0.1667341584005797 1105 32-33 -0.2249605368581271 1105 34-35 -0.1671202806777572 1105 36-37 -0.07399558935439643 1105 38-39 -0.10113835119994263 1105 40-41 -0.11063894659687179 1105 42-43 -0.08071975053107394 1105 44-45 -0.14398290617747733 1105 46-47 -0.1559411030331077 1105 48-49 0.060361054759368926 1105 50-51 -0.0030945553502874645 1105 52-53 0.14251598411043886 1105 54-55 0.019726792025743123 1105 56-57 0.16987272039875023 1105 58-59 0.1486272391515442 1105 60-61 0.19357855708755523 1105 62-63 0.07951443027658911 1105 64-65 -0.06723675175671673 1105 66-67 0.051844758202836516 1105 68-69 0.1425817336023023 1105 70-71 0.12125123234565294 1105 72-73 0.09367811582051999 1105 74-75 -0.016662933753149645 1105 76-77 0.08619003281812354 1105 78-79 -0.028010005485203493 1105 80-81 -0.07274517154802851 1105 82-83 0.07440051875832765 1105 84-85 0.3158161860108706 1105 86-87 0.3188566820865155 1105 88-89 0.1896754487818555 1105 90-91 0.47387182082434265 1105 92-93 0.5503741015035963 1105 94-95 0.758857305511917 1105 96-97 0.8633907224509478 1105 98-99 1.1020072378452426 1105 100-101 1.4426642012690216 1106 1 0.11562653839373027 1106 2 0.029092721008638023 1106 3 0.021636998126297158 1106 4 -0.029740425265906367 1106 5 0.02949344538112797 1106 6 0.020512178437257944 1106 7 0.06052598391345043 1106 8 0.11370920780370852 1106 9 0.030614447440726167 1106 10-11 0.05892988563344659 1106 12-13 0.1150916513029756 1106 14-15 -0.139161001503588 1106 16-17 0.12012034151990747 1106 18-19 -0.20296952297131554 1106 20-21 -0.25853537578462493 1106 22-23 -0.23249832336413334 1106 24-25 -0.06256133643071848 1106 26-27 -0.029243636508837767 1106 28-29 0.08896746895732832 1106 30-31 0.02612203097381638 1106 32-33 -0.10932413479351766 1106 34-35 -0.17139355201351947 1106 36-37 -0.22186534996666296 1106 38-39 -0.09084367735461285 1106 40-41 0.15144946668068826 1106 42-43 0.057559483250180676 1106 44-45 0.11680622793304707 1106 46-47 0.13647402111034523 1106 48-49 0.09455445602304735 1106 50-51 0.16540237443524575 1106 52-53 0.10381793302919107 1106 54-55 0.32073740428880626 1106 56-57 0.06103751082324038 1106 58-59 0.029618606954336713 1106 60-61 0.034269466582976804 1106 62-63 0.11599046925496026 1106 64-65 0.24925675184745444 1106 66-67 0.2394532168661847 1106 68-69 0.08013457243829336 1106 70-71 0.14999945251117808 1106 72-73 0.07959406313742434 1106 74-75 0.18436770164776206 1106 76-77 0.3670011923802825 1106 78-79 0.11717053647439357 1106 80-81 0.009453146508391796 1106 82-83 0.1469315546219967 1106 84-85 0.10271382328060596 1106 86-87 0.26333641422142406 1106 88-89 0.1720639866478315 1106 90-91 0.06705171759394091 1106 92-93 0.46377403031021913 1106 94-95 0.644388919821786 1106 96-97 0.5582203032999296 1106 98-99 0.8559467902168727 1106 100-101 1.4661062899047792 1107 1 0.15220660616678217 1107 2 0.10213555704999777 1107 3 0.09341126174607695 1107 4 0.08818902125420891 1107 5 0.04543423085336684 1107 6 0.023468560399201976 1107 7 0.08109032486401446 1107 8 0.10579583566016737 1107 9 0.09503033935141048 1107 10-11 0.06623123674996378 1107 12-13 0.14515453687079116 1107 14-15 0.1167604512725049 1107 16-17 0.16248121127401305 1107 18-19 0.024361261632037667 1107 20-21 0.09971088800893568 1107 22-23 0.1384298632724139 1107 24-25 0.021569561127705583 1107 26-27 0.08168965482837365 1107 28-29 0.15773267444868821 1107 30-31 0.11673589838192555 1107 32-33 0.09643603116355592 1107 34-35 0.13115825526773506 1107 36-37 -0.056936815776026606 1107 38-39 -0.07890356526841913 1107 40-41 -0.0817365182007066 1107 42-43 -0.14433414347672624 1107 44-45 9.588476219946074E-4 1107 46-47 -0.23323826927109792 1107 48-49 -0.26613554745248535 1107 50-51 -0.2927434245047138 1107 52-53 -0.2549056745822469 1107 54-55 -0.33003644661048526 1107 56-57 -0.4754181977526528 1107 58-59 -0.3305039058617254 1107 60-61 -0.24508527753032894 1107 62-63 -0.19409131456639273 1107 64-65 -0.33395529959055636 1107 66-67 -0.20432176358390208 1107 68-69 -0.3477723916767488 1107 70-71 -0.31917630124675256 1107 72-73 -0.1874176233687912 1107 74-75 -0.1505381329164912 1107 76-77 -0.3174696565205757 1107 78-79 -0.1473784034856358 1107 80-81 -0.013247565977486886 1107 82-83 -0.014662089482619933 1107 84-85 -0.060616505158009204 1107 86-87 -0.023136672058878816 1107 88-89 0.007827302313831552 1107 90-91 0.10714533997642661 1107 92-93 0.2640716234981575 1107 94-95 0.5267445762645693 1107 96-97 0.570593228836465 1107 98-99 0.9573179997051007 1107 100-101 1.4145059119397096 1108 1 0.09059124427608722 1108 2 0.03909272100863603 1108 3 0.017378174596885287 1108 4 0.04764781002820939 1108 5 -0.09847714285416487 1108 6 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-0.8273416546710592 2316 50-51 -0.7584845611314535 2316 52-53 -1.033822804931546 2316 54-55 -0.8164838357854691 2316 56-57 -0.7169932935065546 2316 58-59 -0.7648565639064842 2316 60-61 -0.6072376147586311 2316 62-63 -0.7758558646446119 2316 64-65 -0.6557895682252415 2316 66-67 -0.6195236428501723 2316 68-69 -0.703915372254162 2316 70-71 -0.49192441072115045 2316 72-73 -0.668742266021507 2316 74-75 -0.664897744960701 2316 76-77 -0.6857987523121665 2316 78-79 -0.487071108135094 2316 80-81 -0.3823598547162739 2316 82-83 -0.4518578520070129 2316 84-85 -0.9604432007781796 2316 86-87 -1.0524674711339266 2316 88-89 -1.522298593844404 2316 90-91 -1.9286940454527155 2316 92-93 -1.7039312989672304 2316 94-95 -1.628281002747645 2316 96-97 -1.5015098748693916 2316 98-99 -1.7328828131489864 2316 100-101 -1.791859725581336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 25.0 5 111.0 6 311.0 7 541.0 8 900.0 9 1470.0 10 2302.0 11 3438.0 12 5018.0 13 6797.0 14 7848.0 15 10117.0 16 13505.0 17 18218.0 18 16588.0 19 10823.0 20 9935.0 21 10561.0 22 11244.0 23 12491.0 24 14203.0 25 16282.0 26 19263.0 27 23081.0 28 27290.0 29 32660.0 30 38928.0 31 46838.0 32 56589.0 33 71218.0 34 97123.0 35 149047.0 36 182473.0 37 235463.0 38 157171.0 39 10758.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.97807315122179 19.197604226906304 14.585240131324309 18.239082490547595 2 14.282858525304794 24.78216984353786 38.2536787115934 22.68129291956394 3 22.754136444554494 29.85380561985524 30.00335443675372 17.38870349883655 4 11.65761423068681 17.75701005346654 38.599344711158764 31.98603100468788 5 11.272742145481564 42.22947819836688 32.55676210774746 13.941017548404103 6 25.851944234312207 37.835993872468286 18.3342079377736 17.97785395544591 7 24.908623779852025 37.89862331795442 20.211730836360278 16.981022065833283 8 24.478528954543975 31.675650953818568 20.359613793019893 23.486206298617564 9 26.72857609081664 20.23262981026609 21.629983666997568 31.408810431919697 10-11 23.654458182743355 27.14136868591644 25.997831352852675 23.206341778487534 12-13 30.37238034012323 25.346177115775014 25.686542650943977 18.59489989315778 14-15 25.83113174758583 25.88387136833003 29.31455908844128 18.970437795642866 16-17 18.72448371103341 30.109287238211348 30.793596104808223 20.372632945947018 18-19 18.30988635047125 30.749167637787465 32.892448276932605 18.04849773480868 20-21 20.57242743853544 28.205399053034174 30.4942531607805 20.727920347649885 22-23 19.347338674687027 27.688225169785234 29.966364767358833 22.99807138816891 24-25 20.777339057353913 28.1227532089713 31.24508048599403 19.854827247680753 26-27 22.059542329816182 25.583786749213534 33.47521076218922 18.88146015878106 28-29 19.113512473885745 27.130502733903818 34.65602636299549 19.099958429214947 30-31 18.750127779052413 29.856645154353313 32.63071891713027 18.76250814946401 32-33 18.6561581173962 29.54880175430227 31.584747989420276 20.210292138881254 34-35 20.083374982961516 26.9893376951853 31.79112332833538 21.136163993517805 36-37 21.646903346858096 26.73968650067119 32.982522573352746 18.630887579117967 38-39 22.42207769587166 25.732573408800725 33.58773538753274 18.257613507794883 40-41 19.217270898466417 27.675216115304913 34.6336537467622 18.47385923946647 42-43 18.33818233036588 29.898837452330397 31.960443694587397 19.802536522716327 44-45 19.100019650225978 29.690620632888727 30.08430666316825 21.125053053717053 46-47 19.453041972722897 29.081238786141327 28.963151340854736 22.50256790028104 48-49 20.66047083241678 28.282884118312744 29.80088496412855 21.255760085141926 50-51 21.373138306531914 29.239103191712495 30.425612146847094 18.962146354908498 52-53 19.45520312515381 29.846347109240302 31.01846908958466 19.67998067602123 54-55 17.546089430949717 28.820480085776655 32.08090931738348 21.55252116589015 56-57 20.169738199121323 30.52239777922827 28.524324144229006 20.783539877421397 58-59 20.13220175476457 28.463395962390763 29.598003381711646 21.80639890113302 60-61 19.71379957640883 27.698008424875418 31.268812286415475 21.319379712300275 62-63 22.37859960776296 27.319234001953614 30.276421102049778 20.025745288233647 64-65 20.872650394265424 28.581110167531097 31.235014146759042 19.31122529144444 66-67 19.187853775817352 28.698824375745414 31.167673172670995 20.945648675766236 68-69 18.59179970862524 30.672266998627933 30.326940958750175 20.40899233399665 70-71 19.52197328941515 30.269055664404505 28.30625360857919 21.902717437601158 72-73 19.40935410812493 28.493895758038345 28.454823763344866 23.641926370491863 74-75 19.51231793796166 30.186539051289717 28.8217789169604 21.479364093788217 76-77 20.85593018727545 27.53793165105154 30.276374866085703 21.329763295587306 78-79 19.42522007032241 27.492203038710688 31.863430147340388 21.21914674362651 80-81 19.18512222409423 30.570883916925855 29.845054718306258 20.398939140673658 82-83 18.899734747207198 29.718080881278365 29.066912913335862 22.315271458178575 84-85 19.162428188172544 28.134486411502312 28.969158112095734 23.73392728822941 86-87 20.378996039491387 28.246233726857557 29.68490269276686 21.689867540884197 88-89 21.37073028768817 26.371858670271415 30.104981372189204 22.152429669851212 90-91 19.439720061463643 27.040201828174688 31.411562021002993 22.10851608935868 92-93 18.77896571685807 29.701253450519427 30.13282630432569 21.386954528296815 94-95 18.598373360734254 29.699077036308992 28.853386661133985 22.849162941822772 96-97 18.18269585711446 28.36035626733301 28.369064183017674 25.087883692534856 98-99 19.750704015253223 28.07974018637947 28.048618888280597 24.12093691008671 100-101 20.755174169757066 27.41063400025367 29.334537311002958 22.49965451898631 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11458.0 1 7213.5 2 4500.5 3 5535.0 4 4036.5 5 3578.5 6 6198.5 7 11872.5 8 15856.0 9 15213.5 10 11021.5 11 6919.5 12 5855.0 13 5559.0 14 5245.0 15 4839.0 16 4217.0 17 3892.5 18 3905.0 19 3891.0 20 3932.5 21 4191.5 22 4563.0 23 5014.0 24 5258.5 25 5817.0 26 6980.0 27 7956.5 28 8887.5 29 10050.0 30 11538.5 31 13134.5 32 15833.0 33 18948.0 34 21751.0 35 23870.0 36 27435.5 37 31155.0 38 31402.5 39 30450.5 40 29161.0 41 28445.0 42 27814.5 43 28392.5 44 31940.0 45 34469.5 46 38075.5 47 56729.5 48 136428.5 49 135259.0 50 62208.5 51 45801.0 52 33143.0 53 27381.0 54 24277.0 55 21936.5 56 19564.0 57 17567.0 58 16097.5 59 14835.5 60 13757.5 61 12467.5 62 10452.0 63 7717.0 64 5368.0 65 4114.0 66 3118.0 67 2479.5 68 1878.0 69 1253.0 70 981.0 71 821.5 72 753.5 73 727.5 74 590.5 75 458.0 76 308.5 77 188.5 78 147.5 79 101.5 80 56.5 81 39.0 82 31.0 83 23.5 84 14.5 85 8.5 86 4.5 87 1.5 88 2.5 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2260269460465729 2 0.0 3 0.0 4 0.0 5 0.026350880142113023 6 0.003180278637841227 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0027638135781239232 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.1646505971730345E-4 26-27 0.00117367425920331 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007496371074911462 36-37 0.016772183768615043 38-39 0.0118881844319303 40-41 0.007988557054577367 42-43 0.006890603715322659 44-45 0.003293860017764128 46-47 5.679068996145048E-4 48-49 0.001552278858946313 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.001287255639126211 58-59 4.543255196916038E-4 60-61 0.0019687439186636163 62-63 6.814882795374058E-4 64-65 0.001400837019049112 66-67 0.004846138876710441 68-69 2.271627598458019E-4 70-71 1.1358137992290096E-4 72-73 0.0 74-75 3.0288367979440257E-4 76-77 0.05792650376067949 78-79 0.14053802742460278 80-81 8.707905794089073E-4 82-83 9.086510393832076E-4 84-85 0.0 86-87 0.10328333480989127 88-89 0.34801334808376855 90-91 0.38348859907968796 92-93 3.407441397687029E-4 94-95 1.5144183989720128E-4 96-97 1.1358137992290096E-4 98-99 0.0 100-101 0.0012493951791519107 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1320639.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.05501827599613 #Duplication Level Percentage of deduplicated Percentage of total 1 69.35567967955286 19.45774860953989 2 13.49855143796762 7.5740421458331095 3 5.745315455214763 4.835547902922397 4 3.0938341803728733 3.471902978930499 5 1.8814793839984283 2.6392469251992923 6 1.249627341799193 2.1034990747416495 7 0.8616021624055774 1.6920585089048386 8 0.6440954995490801 1.4456088809069039 9 0.47805171184193074 1.2070574561336942 >10 2.736682741191044 14.512722961735145 >50 0.2735350499830282 5.344715208268135 >100 0.15106300064800818 7.77338679532191 >500 0.01105996968585195 2.1057936360188085 >1k 0.014836544700533102 9.914674309705376 >5k 0.0018882875073405767 3.597747481139196 >10k+ 0.0026975535819151095 12.324247124699188 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 27764 2.102315621452948 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 25114 1.9016551835891564 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18658 1.412800924400991 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 15714 1.1898785360723105 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15575 1.179353328199455 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13661 1.0344234874178333 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12936 0.9795258204550978 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12682 0.9602927067881533 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 10570 0.8003701238567088 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 10173 0.7703089186371143 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 9222 0.6982983237659951 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 8096 0.6130365679038707 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7299 0.5526869947048361 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 6828 0.5170224414090452 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5955 0.45091807829391684 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5106 0.38663101725755483 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5033 0.381103390101307 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4923 0.37277408890696095 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4888 0.37012385670875997 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4762 0.36058302079523624 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4688 0.3549796727190398 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4527 0.34278860460731514 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4354 0.32968888545620717 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4264 0.32287400266083316 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4253 0.3220410725413985 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4096 0.3101528881094682 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3754 0.2842563334870468 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3649 0.27630563689244375 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3473 0.26297875498149004 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3393 0.256921081385602 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3267 0.2473802454720783 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3202 0.24245838567541925 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3138 0.2376122467987088 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3084 0.2335233171214844 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2799 0.2119428549361332 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2644 0.20020611234410007 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2555 0.19346695046867463 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2513 0.1902866718308334 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2493 0.1887722534318614 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2396 0.18142732419684712 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2196 0.166283140207127 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2133 0.16151272225036517 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2090 0.15825672269257535 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2034 0.1540163511754537 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1964 0.14871588677905165 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1923 0.14561132906115903 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1883 0.14258249226321498 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1841 0.13940221362537378 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1783 0.13501040026835492 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1698 0.12857412207272387 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1690 0.12796835471313509 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1653 0.12516668067503686 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1547 0.1171402631604852 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1491 0.11289989164336356 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1448 0.10964389208557374 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1414 0.10706938080732131 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1400 0.1060092879280409 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1379 0.10441914860912027 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.572091994860064E-5 0.0 14-15 0.0 0.0 0.0 2.2716275984580194E-4 0.0 16-17 0.0 0.0 0.0 4.164650597173035E-4 0.0 18-19 0.0 0.0 0.0 5.679068996145048E-4 0.0 20-21 0.0 0.0 0.0 0.0010979533392547092 0.0 22-23 0.0 0.0 0.0 0.0030666972579183258 0.0 24-25 0.0 0.0 0.0 0.00594409221596515 0.0 26-27 0.0 0.0 0.0 0.009654417293446581 0.0 28-29 0.0 0.0 0.0 0.02002818332640487 0.0 30-31 0.0 0.0 0.0 0.043463808050496766 0.0 32-33 0.0 0.0 0.0 0.08068064020523398 0.0 34-35 0.0 0.0 0.0 0.14352900376257252 0.0 36-37 0.0 0.0 0.0 0.23238750332225536 0.0 38-39 0.0 0.0 0.0 0.3407820002286772 0.0 40-41 0.0 0.0 0.0 0.46507789032430513 0.0 42-43 0.0 0.0 0.0 0.5868749900616292 0.0 44-45 0.0 0.0 0.0 0.7204466928509607 0.0 46-47 0.0 0.0 0.0 0.8976715059906606 0.0 48-49 0.0 0.0 0.0 1.1051846871098006 0.0 50-51 0.0 0.0 0.0 1.308457496711819 0.0 52-53 0.0 0.0 0.0 1.504498958458746 0.0 54-55 0.0 0.0 0.0 1.698874559966804 0.0 56-57 0.0 0.0 0.0 1.928952575230627 0.0 58-59 0.0 0.0 0.0 2.2352058359627422 0.0 60-61 0.0 0.0 0.0 2.6395176880282953 0.0 62-63 0.0 0.0 0.0 2.987114571052347 0.0 64-65 0.0 0.0 0.0 3.275346252836695 0.0 66-67 0.0 0.0 0.0 3.607912533250949 0.0 68-69 0.0 0.0 0.0 3.9857977842544403 0.0 70-71 0.0 0.0 0.0 4.439971862106146 0.0 72-73 0.0 0.0 0.0 4.905769101169964 0.0 74-75 0.0 0.0 0.0 5.354415551865422 0.0 76-77 0.0 0.0 0.0 5.776824703798692 0.0 78-79 0.0 0.0 0.0 6.274121845561126 0.0 80-81 0.0 0.0 0.0 6.818896004131334 0.0 82-83 0.0 7.572091994860064E-5 0.0 7.364540953280949 0.0 84-85 0.0 7.572091994860064E-5 0.0 8.063558625786456 0.0 86-87 0.0 7.572091994860064E-5 0.0 8.788775736594179 0.0 88-89 0.0 7.572091994860064E-5 0.0 9.395641049522238 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGTA 75 6.9736816E-6 37.959682 3 TACCTGG 835 0.0 34.095524 2 TATAACG 70 2.0663417E-4 33.892574 2 GCGTATC 80 4.463178E-4 29.722403 1 GTGTACG 100 4.9141385E-5 28.533503 1 CTTTGCG 210 1.2823875E-9 24.854553 2 AGTACTC 580 0.0 24.542898 5 CATTCCG 155 1.4249435E-6 24.488262 9 ACCTGGG 1145 0.0 24.450012 3 CGCGGAA 70 1.7193966E-5 23.857595 82-83 ATGGGGA 1420 0.0 23.724802 5 TGTACGT 100 0.0016640256 23.7248 2 GTAAGCG 140 1.8189894E-12 23.724352 94-95 ATATAGG 750 0.0 23.40847 2 CGTATCA 105 0.0022099852 22.595047 2 CATGGGT 875 0.0 22.233528 4 GATATAG 625 0.0 22.06591 1 GAGTACT 11450 0.0 22.024065 12-13 AGGCTTA 625 0.0 22.01495 6 CTTACTA 630 0.0 21.840227 9 >>END_MODULE