FastQCFastQC Report
Thu 26 May 2016
SRR938717_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938717_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3981065
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97150.24403017785441838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82140.20632669901144543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48480.12177645931427897No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111900.031.9405751
GTATCAA165050.030.7017861
ATCAACG196750.025.4526063
TATCAAC199550.025.2141762
TCAACGC198600.025.1916544
CAACGCA200500.024.9293025
AACGCAG205250.024.4022926
TACATGG157550.022.373122
GTACATG161600.022.2639331
ACATGGG156700.021.950263
ACGCAGA228850.021.7012837
CGCAGAG230950.021.3604588
GAGTACT109100.021.28587312-13
GTACTTT119100.019.53852314-15
GCAGAGT245000.019.3434669
TACCTGG32100.019.3347382
GTGTAGC30450.019.1478161
CATGGGG79950.019.1406214
AGTACTT118350.018.06203512-13
AGAGTAC210200.017.59076110-11
Produced by FastQC (version 0.11.5)