FastQCFastQC Report
Thu 26 May 2016
SRR938717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938717_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3981065
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92870.23327928581924687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84790.21298320926686704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51110.1283827317564521No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114850.030.1561151
GTATCAA166200.028.492291
ATCAACG194950.023.8782043
TCAACGC196850.023.6236274
CAACGCA198700.023.4036755
GTACATG153450.023.2223761
TATCAAC201550.023.2142
TACATGG152600.023.1041582
AACGCAG205250.022.679936
ACATGGG154950.021.4062923
GAGTACT110650.021.18607312-13
ACGCAGA231600.020.0380847
CGCAGAG234100.019.8038238
GTACTTT119350.019.4629214-15
AGTACTT118850.018.78604712-13
CATGGGG75750.018.2291434
GCAGAGT256500.017.9080929
AGAGTAC215000.017.24884610-11
TACCTGG32250.016.7737832
ACTTTTT143450.016.3912716-17
Produced by FastQC (version 0.11.5)