FastQCFastQC Report
Thu 26 May 2016
SRR938716_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938716_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3994535
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97870.24500974456350988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80400.2012749919577623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49270.12334351808157897No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111950.031.6245351
GTATCAA165950.031.2420441
ATCAACG198650.025.719393
TCAACGC200300.025.483844
TATCAAC200750.025.4503462
CAACGCA202650.025.1414995
AACGCAG206450.024.7237636
GTACATG154600.022.7464791
TACATGG154850.022.2718722
ACGCAGA230850.021.9464367
CGCAGAG232350.021.7639248
ACATGGG153200.021.3041023
GAGTACT108800.021.04818312-13
GCAGAGT250250.019.7320429
GTACTTT119050.019.27632314-15
CATGGGG79150.018.5200064
AGTACTT117200.018.44620112-13
AGAGTAC212400.017.52889810-11
TACCTGG29700.017.4101922
CGGACCG1952.2996224E-417.0293065
Produced by FastQC (version 0.11.5)