##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938716_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3994535 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50176703921733 33.0 31.0 34.0 30.0 34.0 2 31.57507719922344 33.0 31.0 34.0 28.0 34.0 3 31.59626664930962 33.0 31.0 34.0 28.0 34.0 4 35.13062046020375 37.0 35.0 37.0 32.0 37.0 5 35.161985062091084 37.0 35.0 37.0 33.0 37.0 6 35.20915625973987 37.0 35.0 37.0 32.0 37.0 7 35.11493052382818 37.0 35.0 37.0 32.0 37.0 8 35.0762899811868 37.0 35.0 37.0 32.0 37.0 9 36.64673435080679 39.0 37.0 39.0 32.0 39.0 10-11 36.52699675932243 39.0 37.0 39.0 32.0 39.0 12-13 36.41806968771083 39.0 37.0 39.0 32.0 39.0 14-15 37.58964197334609 40.0 37.0 41.0 32.0 41.0 16-17 37.4687818482001 40.0 37.0 41.0 32.0 41.0 18-19 37.396338247130146 40.0 37.0 41.0 32.0 41.0 20-21 37.28032236543177 40.0 37.0 41.0 31.0 41.0 22-23 37.30261807694763 40.0 37.0 41.0 31.0 41.0 24-25 37.24772570524479 40.0 37.0 41.0 31.0 41.0 26-27 37.12197640025685 40.0 37.0 41.0 31.0 41.0 28-29 36.95647165439782 40.0 36.5 41.0 30.0 41.0 30-31 36.74179109708639 39.5 36.0 41.0 30.0 41.0 32-33 36.57457801721603 39.0 36.0 41.0 30.0 41.0 34-35 36.303772028533984 39.0 35.5 41.0 29.5 41.0 36-37 36.10558300277754 39.0 35.0 41.0 29.0 41.0 38-39 35.947494389209254 39.0 35.0 41.0 28.0 41.0 40-41 35.851690371970705 39.0 35.0 41.0 27.0 41.0 42-43 35.86361466353405 39.0 35.0 41.0 27.5 41.0 44-45 35.710339626514724 39.0 35.0 41.0 27.0 41.0 46-47 35.44830649875392 39.0 35.0 41.0 26.0 41.0 48-49 35.20096707126111 39.0 35.0 41.0 24.5 41.0 50-51 34.08895328242211 38.0 33.5 40.0 23.5 40.5 52-53 34.16185062842108 38.0 33.5 39.5 23.5 40.5 54-55 34.66115017142171 38.0 34.0 40.0 24.0 41.0 56-57 34.56176325905268 38.0 34.0 40.0 23.0 41.0 58-59 34.365935834834346 38.0 34.0 40.0 23.0 41.0 60-61 34.06934486742512 38.0 34.0 40.0 22.0 41.0 62-63 33.6773460490395 37.5 33.0 40.0 21.5 41.0 64-65 33.28952243502685 37.0 33.0 40.0 20.0 41.0 66-67 32.913921895790125 36.0 32.0 39.0 18.5 41.0 68-69 32.50019263819193 36.0 32.0 39.0 17.5 41.0 70-71 32.09002724972994 35.0 31.5 39.0 14.0 40.0 72-73 31.665366056374523 35.0 31.0 38.0 12.0 40.0 74-75 31.197268893625917 35.0 31.0 37.0 8.0 39.5 76-77 30.73775082706748 35.0 30.5 37.0 7.0 39.0 78-79 30.29966942334965 34.5 30.0 36.0 2.0 39.0 80-81 29.839894005184583 34.0 29.5 36.0 2.0 37.5 82-83 29.422829565894403 34.0 29.0 35.0 2.0 37.0 84-85 29.062588136040866 34.0 29.0 35.0 2.0 37.0 86-87 28.634039256133693 34.0 29.0 35.0 2.0 36.0 88-89 28.189407528035176 34.0 28.0 35.0 2.0 36.0 90-91 27.753050605389614 34.0 27.0 35.0 2.0 35.5 92-93 27.296256385286398 34.0 26.0 35.0 2.0 35.0 94-95 26.789300882330487 33.0 25.0 35.0 2.0 35.0 96-97 26.18973948156669 33.0 24.5 35.0 2.0 35.0 98-99 25.42011222833196 33.0 21.0 35.0 2.0 35.0 100-101 22.922362803179844 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19263002993343292 1101 2 0.3103506294998155 1101 3 0.36220908466678026 1101 4 0.257911123732832 1101 5 0.20611697496498493 1101 6 0.3847392044007947 1101 7 0.38663085979241885 1101 8 0.4169088979664366 1101 9 0.5143673869426308 1101 10-11 0.4140252128560391 1101 12-13 0.3630271360093644 1101 14-15 0.4398517568902278 1101 16-17 0.6010195219916952 1101 18-19 0.6329475461650276 1101 20-21 0.7556525133858258 1101 22-23 0.7157884789054236 1101 24-25 0.630708931220056 1101 26-27 0.6042077034688305 1101 28-29 0.6866119340511005 1101 30-31 0.7582287289357339 1101 32-33 0.7473083787318942 1101 34-35 0.7027961409942876 1101 36-37 0.7623869129421195 1101 38-39 0.659037504890712 1101 40-41 0.7171797149317385 1101 42-43 0.831383110190842 1101 44-45 0.934155282547593 1101 46-47 0.8190876594758691 1101 48-49 0.884060815906146 1101 50-51 0.8559383241048906 1101 52-53 0.878596816488745 1101 54-55 0.8647893602191701 1101 56-57 0.8575506577902203 1101 58-59 0.8340213933285625 1101 60-61 0.6371107543053611 1101 62-63 0.6343579810323092 1101 64-65 0.6063074951540415 1101 66-67 0.4720034331526861 1101 68-69 0.5916741528791718 1101 70-71 0.5654322704221286 1101 72-73 0.5503163818265513 1101 74-75 0.45314852337697076 1101 76-77 0.48472201569736484 1101 78-79 0.43515213717119394 1101 80-81 0.3676829968792248 1101 82-83 0.4652773521566296 1101 84-85 0.3749028261520557 1101 86-87 0.27686004681152454 1101 88-89 0.19496069622524814 1101 90-91 0.23445629882359853 1101 92-93 0.11168566609916653 1101 94-95 0.1845386206813231 1101 96-97 0.06703594116131129 1101 98-99 0.20097985858941314 1101 100-101 0.3426937905808636 1102 1 0.020126838560990734 1102 2 -0.06025308363608772 1102 3 0.06343430781947745 1102 4 0.18383970872069 1102 5 0.24156023053121345 1102 6 0.24421967484099127 1102 7 0.17443225971807408 1102 8 0.25818477575077026 1102 9 0.2544026579166001 1102 10-11 0.2530767687317592 1102 12-13 0.18251890274239457 1102 14-15 0.22494859195564487 1102 16-17 0.41549562070635915 1102 18-19 0.42858484460516877 1102 20-21 0.38539637356439016 1102 22-23 0.2871714364257727 1102 24-25 0.28412085612935556 1102 26-27 0.30811984702846473 1102 28-29 0.3982685515985196 1102 30-31 0.4158137431781057 1102 32-33 0.3235530716261863 1102 34-35 0.3794997049560962 1102 36-37 0.37300742001104226 1102 38-39 0.33902072760944435 1102 40-41 0.428829959981492 1102 42-43 0.4534121493104095 1102 44-45 0.473504174680869 1102 46-47 0.3932501787886977 1102 48-49 0.35456771190929004 1102 50-51 0.49380462103432876 1102 52-53 0.3747770965609831 1102 54-55 0.257256376299388 1102 56-57 0.32716681311572415 1102 58-59 0.2673424598707541 1102 60-61 0.2288249894788308 1102 62-63 0.19457846817036284 1102 64-65 0.054754501463520455 1102 66-67 -0.08184823892664639 1102 68-69 0.07998471856995337 1102 70-71 0.07033246494021483 1102 72-73 -0.051357811005356524 1102 74-75 -0.1372161192648882 1102 76-77 -0.05645053155710755 1102 78-79 -0.04499160844331129 1102 80-81 -0.1265675371937185 1102 82-83 -0.05471227636326148 1102 84-85 -0.11072200396804632 1102 86-87 -0.12145448840091078 1102 88-89 -0.16467690911950328 1102 90-91 -0.2265641897490731 1102 92-93 -0.16041921534931802 1102 94-95 -0.2656703972418555 1102 96-97 -0.19523483145183107 1102 98-99 -0.34114654588985616 1102 100-101 -0.05084415735448644 1103 1 0.050683219298193194 1103 2 0.2251140991532452 1103 3 0.3704850712888188 1103 4 0.2877694872487524 1103 5 0.3126036606646707 1103 6 0.347031996243814 1103 7 0.24404700644272737 1103 8 0.29591609091083626 1103 9 0.3112499212525748 1103 10-11 0.3347926851145928 1103 12-13 0.3385635757132448 1103 14-15 0.4384060553054141 1103 16-17 0.48173521957972554 1103 18-19 0.5560301279605042 1103 20-21 0.5195944276450817 1103 22-23 0.3699196252886878 1103 24-25 0.501108453884143 1103 26-27 0.40769533989165296 1103 28-29 0.44151966885200267 1103 30-31 0.5688175216920115 1103 32-33 0.6482455155821114 1103 34-35 0.6221696116677933 1103 36-37 0.7223070418212956 1103 38-39 0.7379642938942155 1103 40-41 0.7807514141620189 1103 42-43 0.7797484156815173 1103 44-45 0.7139106573858811 1103 46-47 0.7047171034926762 1103 48-49 0.5980942271417362 1103 50-51 0.7478659924513451 1103 52-53 0.7060534144338604 1103 54-55 0.7748437002727897 1103 56-57 0.8067252865943502 1103 58-59 0.7503002361699274 1103 60-61 0.7193110645306646 1103 62-63 0.6947716139362399 1103 64-65 0.5550071104351701 1103 66-67 0.5912254993960246 1103 68-69 0.4404282714770744 1103 70-71 0.33960689289822454 1103 72-73 0.4603648368128681 1103 74-75 0.33144197789122387 1103 76-77 0.36104239066094834 1103 78-79 0.34644132887175516 1103 80-81 0.45008203886620635 1103 82-83 0.3966516773672204 1103 84-85 0.41453055957596874 1103 86-87 0.5354681284137435 1103 88-89 0.605826258001116 1103 90-91 0.47236166289157566 1103 92-93 0.3154040414561017 1103 94-95 0.36299632552532657 1103 96-97 0.3039999792236472 1103 98-99 0.4076379516200497 1103 100-101 0.772126359986057 1104 1 0.07914023138165405 1104 2 0.14236183073697362 1104 3 0.09007818883788588 1104 4 0.20720446950976168 1104 5 0.21123095290460725 1104 6 0.2562478203908185 1104 7 0.2420935885210227 1104 8 0.19351483708284434 1104 9 0.3679745794212579 1104 10-11 0.2598439044579095 1104 12-13 0.2361484680743331 1104 14-15 0.3408347659798565 1104 16-17 0.4451198419841731 1104 18-19 0.3456312617588395 1104 20-21 0.4474619463119467 1104 22-23 0.3762831118543417 1104 24-25 0.28192688320817183 1104 26-27 0.41306908940936893 1104 28-29 0.36195435801163 1104 30-31 0.4286346012328224 1104 32-33 0.4360037867048021 1104 34-35 0.4600940000755571 1104 36-37 0.6750370438299385 1104 38-39 0.5845696086687084 1104 40-41 0.5861380939032586 1104 42-43 0.5788367492925346 1104 44-45 0.6303778658741734 1104 46-47 0.6828770446081691 1104 48-49 0.7118384186333344 1104 50-51 0.7376670186976284 1104 52-53 0.6951395473379662 1104 54-55 0.6243711293504575 1104 56-57 0.5715821378879653 1104 58-59 0.585525578037938 1104 60-61 0.6143239189099248 1104 62-63 0.6255426834112967 1104 64-65 0.6522634014109485 1104 66-67 0.5359772965992207 1104 68-69 0.5212688057338468 1104 70-71 0.6867914682302896 1104 72-73 0.5510522194836334 1104 74-75 0.3723713037605023 1104 76-77 0.34045669802055656 1104 78-79 0.1153010752142869 1104 80-81 0.2244357234171943 1104 82-83 0.28702546236047866 1104 84-85 0.3585582275630621 1104 86-87 0.30438787735455364 1104 88-89 0.4809170876223625 1104 90-91 0.44365075602172865 1104 92-93 0.4790912213046248 1104 94-95 0.512403498631052 1104 96-97 0.4425155797768703 1104 98-99 0.5916643914193997 1104 100-101 0.9889035451999497 1105 1 0.17084903879842628 1105 2 0.19081311006472035 1105 3 0.235805328443071 1105 4 0.2612011540525714 1105 5 0.30249189458680803 1105 6 0.29667359590569475 1105 7 0.2394775908147153 1105 8 0.3097234876744466 1105 9 0.2975670552769145 1105 10-11 0.3686472562831469 1105 12-13 0.3924564957910164 1105 14-15 0.4756830015524187 1105 16-17 0.5564105082890833 1105 18-19 0.5108592018458467 1105 20-21 0.5697062010268041 1105 22-23 0.5308195203078938 1105 24-25 0.5553163224283253 1105 26-27 0.6456559638164663 1105 28-29 0.6910037425450142 1105 30-31 0.7199656091279607 1105 32-33 0.7216407422184048 1105 34-35 0.7287891473468946 1105 36-37 0.8161041669251929 1105 38-39 1.0114967782919635 1105 40-41 0.924335412673976 1105 42-43 0.8167816565155377 1105 44-45 0.9632692264431313 1105 46-47 1.0190625755142761 1105 48-49 1.0106585497892056 1105 50-51 1.1333033584559757 1105 52-53 0.993036045614808 1105 54-55 0.9716744099789807 1105 56-57 0.9783635274551514 1105 58-59 0.967918609805146 1105 60-61 1.0179299596613802 1105 62-63 0.9329250597262657 1105 64-65 0.9966284952077942 1105 66-67 0.9168713491731779 1105 68-69 0.9113475935426578 1105 70-71 0.8656609127228378 1105 72-73 0.9949120572321242 1105 74-75 0.8421279794133518 1105 76-77 0.8308607777839541 1105 78-79 0.7331943936925249 1105 80-81 0.7843475068560721 1105 82-83 0.7347207818508643 1105 84-85 0.7923038042274761 1105 86-87 0.829739675648522 1105 88-89 0.7884089048123251 1105 90-91 0.9525704278626606 1105 92-93 0.9113153292773042 1105 94-95 1.0685601371461573 1105 96-97 0.938878173992233 1105 98-99 0.9937548564493106 1105 100-101 1.2666280457166081 1106 1 0.15059057903533102 1106 2 0.23709579384874857 1106 3 0.2322531601558424 1106 4 0.22236390101311798 1106 5 0.30011829993663497 1106 6 0.2670192674445033 1106 7 0.23324490705392975 1106 8 0.2282332484656706 1106 9 0.2373442613291772 1106 10-11 0.2792561451193407 1106 12-13 0.29904580283458415 1106 14-15 0.38356583951171075 1106 16-17 0.4722620418354566 1106 18-19 0.422581920937688 1106 20-21 0.4498088977455552 1106 22-23 0.3984976773555786 1106 24-25 0.35349803885091546 1106 26-27 0.30143847766364473 1106 28-29 0.22361624492204157 1106 30-31 0.22134692718697124 1106 32-33 0.3137054528213028 1106 34-35 0.3706847870603198 1106 36-37 0.4710600693595879 1106 38-39 0.5434403141593265 1106 40-41 0.5630642060200799 1106 42-43 0.5917952677363019 1106 44-45 0.598098742125444 1106 46-47 0.5783691983088701 1106 48-49 0.6525822314070595 1106 50-51 0.5971758251532364 1106 52-53 0.44014340548432784 1106 54-55 0.4760269784895712 1106 56-57 0.609548961333445 1106 58-59 0.7519772607994923 1106 60-61 0.7884478288346557 1106 62-63 0.6917657858936082 1106 64-65 0.6129167036828775 1106 66-67 0.6404424790184109 1106 68-69 0.7584870751957595 1106 70-71 0.8020775072070307 1106 72-73 0.9162857827638895 1106 74-75 0.7003530797132811 1106 76-77 0.7569016351583535 1106 78-79 0.7655048280938814 1106 80-81 0.8081507539284409 1106 82-83 0.7976059294169531 1106 84-85 0.8982698022124112 1106 86-87 0.9778314429405874 1106 88-89 1.1215709645863896 1106 90-91 1.1078331058255948 1106 92-93 1.201292856233568 1106 94-95 1.2946990046088978 1106 96-97 1.2447948647197258 1106 98-99 1.3514013926900326 1106 100-101 1.785881733532161 1107 1 0.09043590393521583 1107 2 0.11939232677608658 1107 3 0.1861604204233629 1107 4 0.26236123289687185 1107 5 0.23995537293058788 1107 6 0.05316442053283055 1107 7 0.006201795315220693 1107 8 0.05478781299024149 1107 9 0.036999847405326136 1107 10-11 0.17356160798421172 1107 12-13 0.15662607238996884 1107 14-15 0.1965902868145193 1107 16-17 0.35641490976816925 1107 18-19 0.33221160290579377 1107 20-21 0.22941192261920662 1107 22-23 0.27568129498425975 1107 24-25 0.23624490235305018 1107 26-27 0.20452568758766176 1107 28-29 0.2476996902578037 1107 30-31 0.25165643554873185 1107 32-33 0.30342228866923193 1107 34-35 0.33329961319094537 1107 36-37 0.31670644974700934 1107 38-39 0.13274501888034962 1107 40-41 0.17384853592577798 1107 42-43 0.2777883278834068 1107 44-45 0.5007839295073211 1107 46-47 0.6267080036873267 1107 48-49 0.5788666663526456 1107 50-51 0.49578551653539193 1107 52-53 0.44718331273291057 1107 54-55 0.4723512701408126 1107 56-57 0.5546976830272641 1107 58-59 0.6531373928162338 1107 60-61 0.688412637894146 1107 62-63 0.6949972666396818 1107 64-65 0.6749314099545671 1107 66-67 0.4657457623486394 1107 68-69 0.5196176069382688 1107 70-71 0.6151444174424512 1107 72-73 0.5697319505959619 1107 74-75 0.6232455515338806 1107 76-77 0.5421596224015914 1107 78-79 0.5200639201641799 1107 80-81 0.396972034493583 1107 82-83 0.2847693161229259 1107 84-85 0.4356065820917898 1107 86-87 0.4194237650922439 1107 88-89 0.45630385554879993 1107 90-91 0.548995211423172 1107 92-93 0.6969603477469981 1107 94-95 0.6731286600333739 1107 96-97 0.7127177293413638 1107 98-99 0.9131691434852236 1107 100-101 1.2670311999604813 1108 1 0.04034601028608975 1108 2 0.25697188789354186 1108 3 0.10447181162486174 1108 4 0.09102441157022412 1108 5 0.05488470265420631 1108 6 0.16534665621083633 1108 7 0.1332368114357152 1108 8 0.15897253196783367 1108 9 0.10020301453518243 1108 10-11 0.238287280203636 1108 12-13 0.2730222599414418 1108 14-15 0.34430015630909594 1108 16-17 0.4196383428774695 1108 18-19 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2316 90-91 -1.8880580970847127 2316 92-93 -1.6221885091220791 2316 94-95 -1.695893442350009 2316 96-97 -1.8246626199184313 2316 98-99 -2.0783441026087495 2316 100-101 -2.0183710735091402 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21458.0 3 5400.0 4 5147.0 5 5848.0 6 6594.0 7 7863.0 8 9592.0 9 11275.0 10 12940.0 11 15903.0 12 18565.0 13 22027.0 14 23520.0 15 25283.0 16 28797.0 17 33901.0 18 31514.0 19 25880.0 20 26546.0 21 28951.0 22 31297.0 23 35122.0 24 40475.0 25 45613.0 26 52217.0 27 60744.0 28 70957.0 29 84120.0 30 99880.0 31 120472.0 32 147396.0 33 185747.0 34 245250.0 35 337512.0 36 502433.0 37 751757.0 38 699501.0 39 116980.0 40 58.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.581727679503864 18.862616683536576 14.203208094342957 23.352447542616602 2 15.221915425611545 19.997452231216037 40.9887348998935 23.791897443278916 3 19.4379909809259 24.336249223034205 29.63092933386377 26.594830462176127 4 12.726735193755529 16.33330926058549 37.408598785529875 33.531356760129114 5 13.735363937947614 37.274994855734725 34.511068953229845 14.478572253087812 6 31.995423717504607 35.8640178877361 17.803028212716367 14.337530182042931 7 28.464614312874037 32.495944678997255 20.906710552467224 18.132730455661488 8 25.783275368705727 35.530116593476805 19.96755520108096 18.71905283673651 9 26.26240365527052 17.164116612035173 20.57491183495122 35.99856789774309 10-11 24.949648849565424 26.560459197976442 27.558125051975484 20.931766900482646 12-13 25.71812733149367 23.876690287827945 27.92120108041945 22.48398130025894 14-15 22.79341168587278 25.361538609112227 26.193497778286577 25.65155192672841 16-17 22.215256588141767 28.54800967048715 26.876349548015572 22.36038419335551 18-19 22.022444505392066 27.30273483911877 29.023260272302043 21.65156038318712 20-21 22.966075165216886 26.47691829935348 28.8902537464462 21.666752788983434 22-23 22.54761363711936 26.44108868383802 28.6930581580079 22.318239521034723 24-25 22.359802774303446 26.577064702590008 28.93814972747157 22.124982795634978 26-27 22.225930329506852 26.76241789584771 28.8139572097538 22.19769456489164 28-29 22.105717844657853 26.856026740868728 28.925731807771125 22.112523606702293 30-31 22.298040162864904 26.735423367653937 28.780398766436694 22.186137703044466 32-33 22.05533115014628 26.741683622485223 28.740651725480284 22.462333501888214 34-35 22.537848143119447 26.816504845587414 28.555248138496463 22.090398872796673 36-37 22.52604713229563 26.60220874353173 28.581881285250756 22.289862838921884 38-39 22.330179677225846 26.848786600090317 28.653512736634156 22.167520986049677 40-41 22.557478934282248 26.6495256170558 28.679081995052375 22.113913453609573 42-43 22.271702161423967 26.774816394621237 28.48961417583924 22.463867268115557 44-45 22.341675967488634 26.967001328712787 28.413167098889957 22.278155604908623 46-47 22.45712935712257 27.020920722926085 27.91971509212507 22.60223482782627 48-49 22.505140376683183 26.986553385446616 27.994807525116798 22.5134987127534 50-51 22.45405269807021 27.224546182574638 27.930112945981282 22.391288173373866 52-53 22.581368023679108 27.190830793446395 27.86113220788619 22.366668974988308 54-55 22.409716773808505 27.003626522817235 27.990857095748016 22.59579960762624 56-57 22.370707159040425 27.389584300811983 27.729609068265294 22.510099471882306 58-59 22.432363072741847 27.025125831396668 27.899881557888722 22.642629537972763 60-61 22.43841502219887 27.014461538616093 28.11348020736694 22.4336432318181 62-63 22.451316562597228 27.002915753765826 28.083606889442116 22.46216079419483 64-65 22.31701139456103 26.938363783773596 28.060712332899673 22.683912488765703 66-67 22.169385703291933 27.162996128543714 27.8618267973963 22.805791370768052 68-69 22.30551365213456 27.026914567305276 27.950972997435507 22.716598783124653 70-71 22.260179690024025 26.918078495903735 27.864172745797827 22.957569068274413 72-73 22.287926888991013 26.928058501678848 27.873748155984362 22.910266453345777 74-75 22.214964068999183 27.18309802503933 27.699614017640457 22.90232388832103 76-77 22.367753324028392 26.95246059412381 27.787781470095368 22.89200461175243 78-79 22.350994292767556 27.05625714911778 27.720949201811678 22.871799356302983 80-81 22.30890025579947 27.15712762215816 27.620700543980682 22.913271578061686 82-83 22.498659737094833 27.029464671474102 27.609101673236236 22.862773918194826 84-85 22.42378993604402 26.963882941904764 27.575707383917354 23.036619738133858 86-87 22.359949115931403 26.95424316859513 27.770547182180977 22.915260533292482 88-89 22.48846088060381 26.834637353003238 27.632507119775223 23.04439464661773 90-91 22.384470123825757 26.943556828253527 27.730978990134187 22.940994057786526 92-93 22.474250389040986 26.899588458744056 27.657583596674485 22.96857755554047 94-95 22.40032181625046 26.833177244071226 27.61042634367713 23.15607459600118 96-97 22.529602829179915 26.6044561192757 27.60683027912406 23.259110772420325 98-99 22.41039858162305 26.658175579458877 27.492681339855345 23.43874449906273 100-101 22.798106855109822 26.210329603764173 27.834445333933218 23.157118207192795 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3235.0 1 2137.0 2 936.0 3 856.5 4 899.5 5 963.5 6 1204.0 7 1884.5 8 2762.0 9 3210.5 10 3156.5 11 3082.5 12 3246.5 13 3502.0 14 3784.0 15 4079.0 16 4359.5 17 4657.5 18 5022.0 19 5444.5 20 6145.5 21 7078.0 22 8464.0 23 10540.5 24 13103.5 25 16561.5 26 21611.0 27 27919.0 28 33522.0 29 39397.5 30 46273.5 31 54463.5 32 63121.0 33 70352.0 34 78177.0 35 86129.0 36 95449.0 37 104956.5 38 110534.5 39 115183.0 40 119724.5 41 124656.5 42 129520.5 43 134621.5 44 140448.5 45 145326.5 46 147269.0 47 149470.0 48 160320.5 49 161873.0 50 152314.0 51 146406.0 52 140799.5 53 137237.0 54 132748.5 55 123059.0 56 111809.0 57 99602.0 58 89074.0 59 79569.5 60 66991.0 61 54498.0 62 44030.5 63 34012.0 64 25231.0 65 19069.5 66 14220.5 67 10839.0 68 8085.5 69 5930.5 70 4598.5 71 3484.5 72 2719.0 73 2254.5 74 1815.0 75 1447.0 76 1072.0 77 716.5 78 506.5 79 366.0 80 251.0 81 175.5 82 134.0 83 112.0 84 85.0 85 61.0 86 41.5 87 31.5 88 26.5 89 17.5 90 17.0 91 15.5 92 12.0 93 7.5 94 6.5 95 4.5 96 4.0 97 4.5 98 3.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27815502930879316 2 0.16853025446015618 3 0.19709428006013216 4 0.16580152633535566 5 0.23820044135299856 6 0.19759496411972857 7 0.2415049561463349 8 0.24836432776280593 9 0.1500299784580683 10-11 0.20729571777440928 12-13 0.21255290040017175 14-15 0.1742255356380655 16-17 0.2876054409336756 18-19 0.233519045395772 20-21 0.19919715311043715 22-23 0.24479695383818142 24-25 0.23418245177473726 26-27 0.344670906626178 28-29 0.3158064705904442 30-31 0.29044682297188534 32-33 0.19283846555356254 34-35 0.3609681727660416 36-37 0.40239977869764565 38-39 0.3841248105223762 40-41 0.34641078373327555 42-43 0.37913048702790186 44-45 0.38793000937530897 46-47 0.3931621577980916 48-49 0.41232584017914475 50-51 0.40487816479264793 52-53 0.40494075030009746 54-55 0.3988073705700413 56-57 0.3784921148519164 58-59 0.3532200869437869 60-61 0.3204628323446909 62-63 0.38705381227101526 64-65 0.41957324194180295 66-67 0.42463015094372686 68-69 0.42896106805923595 70-71 0.4288358970443368 72-73 0.42064971266993534 74-75 0.4163938981633657 76-77 0.4006473844890582 78-79 0.3760888313658536 80-81 0.38656564531290877 82-83 0.3964917067944078 84-85 0.3970674934629438 86-87 0.39269902504296494 88-89 0.40063486738756826 90-91 0.4207874007863243 92-93 0.4206997810758949 94-95 0.4283352129847404 96-97 0.4287858286383772 98-99 0.42635751094933455 100-101 0.39979622158774425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3994535.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.687968856666224 #Duplication Level Percentage of deduplicated Percentage of total 1 73.6597650331399 39.54643171088566 2 14.329820306588708 15.386778926835158 3 5.179557562200152 8.342397752721354 4 2.3897448840562703 5.132021956423619 5 1.2805290983986215 3.437450317744003 6 0.7588794824109427 2.4445618810585 7 0.48285885727348316 1.8146597904024941 8 0.33795428709621494 1.4515263392478737 9 0.23780820198571728 1.1490695407862082 >10 1.2061375952634728 11.711920255540335 >50 0.08704777177585508 3.240032811732279 >100 0.046716006396129967 4.510538126367645 >500 0.002713132061595174 0.9608202998256888 >1k 3.742250822873774E-4 0.42407889966833606 >5k 9.355627057184414E-5 0.44771139076083255 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9787 0.24500974456350988 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8040 0.2012749919577623 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4927 0.12334351808157897 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.503420297982118E-5 2 5.006840595964236E-5 0.0 0.0 0.0 2.503420297982118E-5 3 5.006840595964236E-5 0.0 0.0 0.0 2.503420297982118E-5 4 5.006840595964236E-5 0.0 0.0 5.006840595964236E-5 2.503420297982118E-5 5 1.0013681191928473E-4 0.0 0.0 5.006840595964236E-5 2.503420297982118E-5 6 1.0013681191928473E-4 0.0 0.0 5.006840595964236E-5 2.503420297982118E-5 7 1.2517101489910591E-4 0.0 0.0 5.006840595964236E-5 2.503420297982118E-5 8 1.2517101489910591E-4 0.0 0.0 5.006840595964236E-5 2.503420297982118E-5 9 1.2517101489910591E-4 0.0 0.0 1.7523942085874826E-4 2.503420297982118E-5 10-11 1.2517101489910591E-4 0.0 0.0 2.0027362383856945E-4 2.503420297982118E-5 12-13 1.376881163890165E-4 0.0 0.0 2.1279072532848003E-4 2.503420297982118E-5 14-15 1.5020521787892707E-4 0.0 0.0 2.3782492830830125E-4 2.503420297982118E-5 16-17 1.5020521787892707E-4 0.0 0.0 2.5034202979821183E-4 2.503420297982118E-5 18-19 1.7523942085874826E-4 0.0 0.0 2.5034202979821183E-4 5.006840595964236E-5 20-21 2.0027362383856945E-4 0.0 0.0 3.629959432074071E-4 5.006840595964236E-5 22-23 2.0027362383856945E-4 0.0 0.0 5.382353640661553E-4 8.761971042937414E-5 24-25 3.504788417174965E-4 0.0 0.0 6.634063789652614E-4 1.2517101489910591E-4 26-27 3.504788417174965E-4 0.0 0.0 0.0010639536266424002 1.2517101489910591E-4 28-29 5.006840595964237E-4 0.0 0.0 0.003329548996316217 1.2517101489910591E-4 30-31 5.006840595964237E-4 0.0 0.0 0.011127703224530515 1.2517101489910591E-4 32-33 5.006840595964237E-4 0.0 0.0 0.027149593131616072 1.5020521787892707E-4 34-35 5.006840595964237E-4 0.0 0.0 0.052421621039745556 1.5020521787892707E-4 36-37 5.006840595964237E-4 0.0 0.0 0.09620644205145279 1.5020521787892707E-4 38-39 5.006840595964237E-4 0.0 0.0 0.15820364573097995 1.7523942085874826E-4 40-41 5.132011610863342E-4 0.0 0.0 0.22614647261821463 1.7523942085874826E-4 42-43 5.382353640661555E-4 0.0 0.0 0.29540359516188996 1.7523942085874826E-4 44-45 5.50752465556066E-4 0.0 0.0 0.38397460530449723 2.0027362383856945E-4 46-47 6.133379730056189E-4 0.0 0.0 0.4907579981149245 2.2530782681839064E-4 48-49 6.258550744955296E-4 0.0 0.0 0.6060655370399808 2.2530782681839064E-4 50-51 6.258550744955296E-4 0.0 0.0 0.7286079606262056 2.2530782681839064E-4 52-53 6.258550744955296E-4 0.0 0.0 0.8512880723288192 2.5034202979821183E-4 54-55 6.258550744955296E-4 0.0 0.0 0.9931068322095062 2.75376232778033E-4 56-57 6.508892774753507E-4 0.0 0.0 1.1612490565234752 2.75376232778033E-4 58-59 6.508892774753507E-4 0.0 0.0 1.3459639232100857 3.0041043575785415E-4 60-61 6.508892774753507E-4 0.0 0.0 1.5479023215468133 3.504788417174965E-4 62-63 6.508892774753507E-4 0.0 0.0 1.7515931140921284 3.504788417174965E-4 64-65 6.508892774753507E-4 0.0 0.0 1.963582744925254 3.504788417174965E-4 66-67 6.508892774753507E-4 0.0 0.0 2.2132989196489703 3.504788417174965E-4 68-69 6.759234804551719E-4 0.0 0.0 2.4977250168042087 3.504788417174965E-4 70-71 6.759234804551719E-4 0.0 0.0 2.802203510546284 3.755130446973177E-4 72-73 6.759234804551719E-4 0.0 0.0 3.111338366042606 4.506156536367813E-4 74-75 6.759234804551719E-4 0.0 0.0 3.427270007647949 4.506156536367813E-4 76-77 6.759234804551719E-4 0.0 0.0 3.773780928193144 4.506156536367813E-4 78-79 6.759234804551719E-4 0.0 0.0 4.166454919033129 4.6313275512669186E-4 80-81 7.510260893946354E-4 0.0 0.0 4.592061403893069 4.88166958106513E-4 82-83 7.760602923744566E-4 0.0 0.0 5.025315837763344 5.382353640661555E-4 84-85 7.760602923744566E-4 0.0 0.0 5.463113979474457 5.50752465556066E-4 86-87 7.760602923744566E-4 0.0 0.0 5.9231925618376104 5.757866685358871E-4 88-89 7.760602923744566E-4 0.0 0.0 6.419458084608095 5.757866685358871E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11195 0.0 31.624535 1 GTATCAA 16595 0.0 31.242044 1 ATCAACG 19865 0.0 25.71939 3 TCAACGC 20030 0.0 25.48384 4 TATCAAC 20075 0.0 25.450346 2 CAACGCA 20265 0.0 25.141499 5 AACGCAG 20645 0.0 24.723763 6 GTACATG 15460 0.0 22.746479 1 TACATGG 15485 0.0 22.271872 2 ACGCAGA 23085 0.0 21.946436 7 CGCAGAG 23235 0.0 21.763924 8 ACATGGG 15320 0.0 21.304102 3 GAGTACT 10880 0.0 21.048183 12-13 GCAGAGT 25025 0.0 19.732042 9 GTACTTT 11905 0.0 19.276323 14-15 CATGGGG 7915 0.0 18.520006 4 AGTACTT 11720 0.0 18.446201 12-13 AGAGTAC 21240 0.0 17.528898 10-11 TACCTGG 2970 0.0 17.410192 2 CGGACCG 195 2.2996224E-4 17.029306 5 >>END_MODULE