FastQCFastQC Report
Thu 26 May 2016
SRR938716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938716_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3994535
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94330.2361476367086532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83110.20805926096529384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51140.1280249140388055No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122800.030.6066321
GTATCAA174450.026.5628571
GTACATG157800.023.0342031
ATCAACG202750.022.5670033
TACATGG156900.022.5064872
TCAACGC205000.022.2961654
CAACGCA208750.021.8728985
TATCAAC210450.021.8540822
ACATGGG156050.021.4732953
AACGCAG214350.021.3163936
GAGTACT115550.020.42838712-13
ACGCAGA238350.019.1287487
GTACTTT123850.018.98272514-15
CGCAGAG239850.018.9695578
AGTACTT124250.018.32971812-13
GTGTACG4700.018.220551
TACCTGG30800.017.5675772
GCAGAGT261500.017.163189
CATGGGA77050.016.5705664
AGAGTAC224300.016.5631710-11
Produced by FastQC (version 0.11.5)