##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938716_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3994535 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.136191571734884 33.0 31.0 34.0 30.0 34.0 2 32.340032319156045 34.0 31.0 34.0 30.0 34.0 3 32.42310081148369 34.0 31.0 34.0 30.0 34.0 4 36.01755898997005 37.0 35.0 37.0 35.0 37.0 5 35.91805629441224 37.0 35.0 37.0 35.0 37.0 6 35.94252847953516 37.0 35.0 37.0 35.0 37.0 7 35.864567965983525 37.0 35.0 37.0 35.0 37.0 8 35.84862167936944 37.0 35.0 37.0 35.0 37.0 9 37.5199664040996 39.0 37.0 39.0 35.0 39.0 10-11 37.44650441165242 39.0 37.0 39.0 34.0 39.0 12-13 37.3735512393808 39.0 37.0 39.0 34.0 39.0 14-15 38.63050667975121 40.0 38.0 41.0 34.0 41.0 16-17 38.54999142578548 40.0 38.0 41.0 34.0 41.0 18-19 38.68042312809877 40.0 38.0 41.0 34.0 41.0 20-21 38.68386783442879 40.0 38.0 41.0 34.0 41.0 22-23 38.61304131770031 40.0 38.0 41.0 34.0 41.0 24-25 38.54451782247496 40.0 38.0 41.0 34.0 41.0 26-27 38.38171989981312 40.0 38.0 41.0 34.0 41.0 28-29 38.222791513905875 40.0 38.0 41.0 33.0 41.0 30-31 38.02546566746818 40.0 37.5 41.0 33.0 41.0 32-33 37.906799164358304 40.0 37.0 41.0 33.0 41.0 34-35 37.90279031226413 40.0 37.0 41.0 33.0 41.0 36-37 37.80948095335252 40.0 37.0 41.0 33.0 41.0 38-39 37.70200211038331 40.0 37.0 41.0 33.0 41.0 40-41 37.54363586750398 40.0 37.0 41.0 32.0 41.0 42-43 37.37223068517362 40.0 37.0 41.0 32.0 41.0 44-45 37.16196478438667 40.0 36.5 41.0 31.0 41.0 46-47 36.90324818783663 40.0 36.0 41.0 31.0 41.0 48-49 36.64173627218187 39.5 36.0 41.0 30.0 41.0 50-51 36.4301075093847 39.0 35.5 41.0 30.0 41.0 52-53 36.215137932199866 39.0 35.0 41.0 29.5 41.0 54-55 35.95204748487622 39.0 35.0 40.0 29.0 41.0 56-57 35.696584333345434 38.5 35.0 40.0 28.0 41.0 58-59 35.40498380912922 38.0 34.5 40.0 28.0 41.0 60-61 35.057495678470715 38.0 34.0 40.0 27.5 41.0 62-63 34.70344708457931 37.5 34.0 40.0 26.5 41.0 64-65 34.3141199163357 37.0 33.5 39.5 26.0 41.0 66-67 33.917628334712305 36.0 33.0 39.0 26.0 40.5 68-69 33.58067384564161 36.0 33.0 39.0 25.5 40.0 70-71 33.15690124132095 35.5 32.5 38.5 25.0 40.0 72-73 32.67419274083216 35.0 32.0 38.0 23.5 40.0 74-75 32.17781506483233 35.0 31.5 37.0 22.0 39.0 76-77 30.212444627472284 33.0 29.5 35.5 20.0 37.5 78-79 31.168909898148343 34.0 31.0 36.0 20.0 38.5 80-81 31.220641075869906 34.5 31.0 36.0 20.0 37.5 82-83 30.90249315627476 34.0 31.0 35.0 20.0 37.0 84-85 30.501269359262093 34.0 31.0 35.0 18.0 36.5 86-87 29.92045770033308 34.0 30.0 35.0 12.5 36.0 88-89 29.32785330457738 34.0 29.0 35.0 6.0 36.0 90-91 28.819400505941243 34.0 29.0 35.0 2.0 35.0 92-93 28.531838749691765 34.0 29.0 35.0 2.0 35.0 94-95 28.20120702409667 34.0 29.0 35.0 2.0 35.0 96-97 27.606364069910516 33.0 27.5 35.0 2.0 35.0 98-99 26.785185384531616 33.0 26.5 35.0 2.0 35.0 100-101 23.06243705462588 28.5 12.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17657500416238037 1101 2 0.21426231666925588 1101 3 0.10430633023500491 1101 4 0.14994197663077813 1101 5 0.04424082719921074 1101 6 0.10815368939991288 1101 7 0.2217497970125919 1101 8 0.23190402171110236 1101 9 0.33020608601982815 1101 10-11 0.2962720981806797 1101 12-13 0.2389130047338739 1101 14-15 0.3381792183247967 1101 16-17 0.36792637207935996 1101 18-19 0.4254969262241204 1101 20-21 0.3339402238192193 1101 22-23 0.2566166923235471 1101 24-25 0.2859299941801794 1101 26-27 0.16543448263165317 1101 28-29 0.31890562138577394 1101 30-31 0.2831368755419632 1101 32-33 0.3610387036515874 1101 34-35 0.47966685418921884 1101 36-37 0.4493650358315975 1101 38-39 0.5383437838005989 1101 40-41 0.5192552653319638 1101 42-43 0.6598713206627309 1101 44-45 0.6700756512913202 1101 46-47 0.6753191404738956 1101 48-49 0.6199206808687805 1101 50-51 0.572431310601992 1101 52-53 0.4900614705578832 1101 54-55 0.5424056105484993 1101 56-57 0.5739205935779381 1101 58-59 0.6363222014807235 1101 60-61 0.6501791305250606 1101 62-63 0.49637339691721394 1101 64-65 0.5186875995491036 1101 66-67 0.587947371844173 1101 68-69 0.6398716460637459 1101 70-71 0.6998336202035915 1101 72-73 0.6063069716067773 1101 74-75 0.4966130558329027 1101 76-77 0.48860834640631623 1101 78-79 0.39867521207650825 1101 80-81 0.5600017663260495 1101 82-83 0.7203964875446118 1101 84-85 0.7497031424237974 1101 86-87 0.763851209927509 1101 88-89 0.7319289593795268 1101 90-91 0.5554311641442098 1101 92-93 0.7531135753806772 1101 94-95 0.7719950237785866 1101 96-97 0.6723205399724534 1101 98-99 0.5761852959967406 1101 100-101 1.0911688940392885 1102 1 0.20259964414508147 1102 2 0.15529775595402384 1102 3 0.1047481140424864 1102 4 0.09786835948838757 1102 5 -0.11515192693151022 1102 6 0.022424638521634677 1102 7 0.041715081950982835 1102 8 0.09977864067416675 1102 9 0.16581357910358463 1102 10-11 0.1456558424323049 1102 12-13 0.18852313596762116 1102 14-15 0.2838647876341369 1102 16-17 0.3303692405563865 1102 18-19 0.2370393383662659 1102 20-21 0.2137327263291553 1102 22-23 0.16892590486727954 1102 24-25 0.19099063473979783 1102 26-27 0.18288154863126493 1102 28-29 0.1788456801997711 1102 30-31 0.19538851144857716 1102 32-33 0.21563540716815766 1102 34-35 0.27788005067759514 1102 36-37 0.2196525706113377 1102 38-39 0.30551369418598995 1102 40-41 0.37190941380873 1102 42-43 0.41980431529087525 1102 44-45 0.31548250228349417 1102 46-47 0.43735304190524715 1102 48-49 0.22374266159220468 1102 50-51 0.22199286995409295 1102 52-53 0.18578597935039198 1102 54-55 0.2309431588417894 1102 56-57 0.18613752338926304 1102 58-59 0.2611244619177313 1102 60-61 0.29007678116770563 1102 62-63 0.057287685734955573 1102 64-65 0.07291252706914264 1102 66-67 0.2380300670097526 1102 68-69 0.2098899284072715 1102 70-71 0.24095566781581823 1102 72-73 0.2149098946810568 1102 74-75 0.16690883323022376 1102 76-77 0.10712601122600773 1102 78-79 0.09198736224560022 1102 80-81 0.25982158155307644 1102 82-83 0.2305045177114451 1102 84-85 0.3227179076609872 1102 86-87 0.3387495445729165 1102 88-89 0.1823004599816791 1102 90-91 0.054375699205500894 1102 92-93 0.2749321107283791 1102 94-95 0.3808536793468136 1102 96-97 0.44044859861121566 1102 98-99 0.5474754328049762 1102 100-101 1.2367897200533235 1103 1 0.20438466817692813 1103 2 0.19475306547003157 1103 3 0.16093877576270188 1103 4 0.08422058255072784 1103 5 -0.034299762173191084 1103 6 0.05654361092475568 1103 7 0.04117258201880958 1103 8 0.08157143314463156 1103 9 0.14274505086054035 1103 10-11 0.22597490607918047 1103 12-13 0.13381899173306522 1103 14-15 0.19167711248319108 1103 16-17 0.16644989095979668 1103 18-19 0.04795204594157809 1103 20-21 0.2019426889663336 1103 22-23 0.22925850156281768 1103 24-25 0.1522836280004114 1103 26-27 0.20547863202681071 1103 28-29 0.10604817948405554 1103 30-31 0.22270251834192578 1103 32-33 0.29039353321623906 1103 34-35 0.24882936102032716 1103 36-37 0.37895988754942067 1103 38-39 0.22543624289052389 1103 40-41 0.3128472243810094 1103 42-43 0.2825027392778168 1103 44-45 0.3529743354915169 1103 46-47 0.38048043308273094 1103 48-49 0.38191726398605397 1103 50-51 0.33806110012920243 1103 52-53 0.3550573307610776 1103 54-55 0.3062254571216343 1103 56-57 0.2778517172815427 1103 58-59 0.31201848087790296 1103 60-61 0.34326148071465923 1103 62-63 0.3097086809710632 1103 64-65 0.3572044221088788 1103 66-67 0.27921908299645537 1103 68-69 0.2989666252280685 1103 70-71 0.16131607624846822 1103 72-73 0.35908902642927387 1103 74-75 0.3865893497693591 1103 76-77 0.34437759177869154 1103 78-79 0.23607513745638542 1103 80-81 0.46179612967187467 1103 82-83 0.48987666144898867 1103 84-85 0.6000257896561578 1103 86-87 0.5504842667428385 1103 88-89 0.5694857882470821 1103 90-91 0.5089012909098258 1103 92-93 0.6322951688139042 1103 94-95 0.6171354895772296 1103 96-97 0.7304838623599856 1103 98-99 0.9072435551254934 1103 100-101 1.5171368673765144 1104 1 0.15663049019784836 1104 2 0.12964211142994486 1104 3 0.10108915047005951 1104 4 0.05538489963552706 1104 5 0.005810720898487887 1104 6 0.03846980075609707 1104 7 0.0571327226781122 1104 8 0.017597837223782165 1104 9 0.059587627285253575 1104 10-11 0.09095976291579433 1104 12-13 0.020432613900929653 1104 14-15 0.050226124880786926 1104 16-17 0.16616431284423783 1104 18-19 0.13900100390257109 1104 20-21 0.1159739815752161 1104 22-23 0.12692758717214758 1104 24-25 0.0711828072431544 1104 26-27 0.10607432754683543 1104 28-29 0.09463355675909924 1104 30-31 -0.06473791027112696 1104 32-33 0.00589081591203211 1104 34-35 0.04454360356978526 1104 36-37 0.07632785308577894 1104 38-39 0.059520403843791314 1104 40-41 0.18602271112379754 1104 42-43 0.19120325059048326 1104 44-45 0.15013163747132552 1104 46-47 0.11581456865263817 1104 48-49 0.18232571592391622 1104 50-51 0.10529643362254859 1104 52-53 0.06894289228876005 1104 54-55 0.1107176027142387 1104 56-57 -0.01585497656183321 1104 58-59 0.12860222040619362 1104 60-61 0.20345652769284328 1104 62-63 0.14152522079432117 1104 64-65 0.054533333966347186 1104 66-67 -0.011133887639822149 1104 68-69 0.032821583595691095 1104 70-71 -0.06252545156814904 1104 72-73 -0.0032525660996256534 1104 74-75 0.03795988006510953 1104 76-77 0.0368691687839835 1104 78-79 0.00518749412602304 1104 80-81 0.17144309787086343 1104 82-83 0.2118677727460394 1104 84-85 0.22086368281997437 1104 86-87 0.3620144541050756 1104 88-89 0.2780886745372051 1104 90-91 0.2772310143009058 1104 92-93 0.27668863857394044 1104 94-95 0.3795339775362194 1104 96-97 0.6061894280811195 1104 98-99 0.8170083938720261 1104 100-101 1.6249686621722788 1105 1 0.14731262194253247 1105 2 0.16519355874342523 1105 3 0.15029881898206554 1105 4 0.06257961491606778 1105 5 -0.014172701815567734 1105 6 0.027498052376778048 1105 7 0.11199651569198465 1105 8 0.13878280048051295 1105 9 0.17139723576112686 1105 10-11 0.17337860348713008 1105 12-13 0.22071518783484834 1105 14-15 0.2406424235374871 1105 16-17 0.1903193571305124 1105 18-19 0.18944987986059658 1105 20-21 0.13827586713325246 1105 22-23 0.1802026835282291 1105 24-25 0.12105308974012274 1105 26-27 0.19078409034636934 1105 28-29 0.2630807422765358 1105 30-31 0.28759014969573116 1105 32-33 0.276379711150895 1105 34-35 0.3047202820320152 1105 36-37 0.34876503642883705 1105 38-39 0.43786111661898275 1105 40-41 0.43964628646818227 1105 42-43 0.38755617552414634 1105 44-45 0.46377487368851433 1105 46-47 0.4141025285207931 1105 48-49 0.3437730149712266 1105 50-51 0.30693126381842006 1105 52-53 0.4486935883808272 1105 54-55 0.5405050276450041 1105 56-57 0.5006460615630388 1105 58-59 0.61928675744484 1105 60-61 0.5283920177089456 1105 62-63 0.39633977744092164 1105 64-65 0.39559776313236483 1105 66-67 0.33927162086818186 1105 68-69 0.3688761462459027 1105 70-71 0.3987530439493483 1105 72-73 0.5156878008905039 1105 74-75 0.5494452789686832 1105 76-77 0.5349719931763559 1105 78-79 0.3245730088685477 1105 80-81 0.4264249427120852 1105 82-83 0.4952389413532927 1105 84-85 0.4887605130273869 1105 86-87 0.4871107861280066 1105 88-89 0.5147569719931759 1105 90-91 0.514759685404858 1105 92-93 0.6443967494175808 1105 94-95 0.7463372167936981 1105 96-97 0.8417055897322676 1105 98-99 1.1014965809923503 1105 100-101 1.7169128276026235 1106 1 0.13169708790145762 1106 2 0.08114055100071482 1106 3 0.06512866826133035 1106 4 0.058664674468360545 1106 5 0.01774224002697622 1106 6 0.06936545839658947 1106 7 0.06308341409578588 1106 8 0.10264158040369153 1106 9 0.09212448642471571 1106 10-11 0.04616146232238094 1106 12-13 0.09904366018396615 1106 14-15 0.11467109846247325 1106 16-17 0.12874046830826558 1106 18-19 0.09321976956204736 1106 20-21 0.06474429816748284 1106 22-23 0.16654735125403874 1106 24-25 0.08625918872937888 1106 26-27 0.16037326976294963 1106 28-29 0.21745139127721558 1106 30-31 0.16898402355376874 1106 32-33 0.09709375160748834 1106 34-35 0.07061771712474041 1106 36-37 0.09881504691330889 1106 38-39 0.16151747641829672 1106 40-41 0.208221069606914 1106 42-43 0.1707102208347422 1106 44-45 0.2345634562106298 1106 46-47 0.22041264862034637 1106 48-49 0.24935487664216538 1106 50-51 0.15505206683162243 1106 52-53 0.11601867315165038 1106 54-55 0.12466877911597152 1106 56-57 0.1788448379247498 1106 58-59 0.16777414515517108 1106 60-61 0.15536215764299754 1106 62-63 0.32230627291539093 1106 64-65 0.3552017289143876 1106 66-67 0.36526832604612736 1106 68-69 0.21110666732202077 1106 70-71 0.3226802391451784 1106 72-73 0.34892584986672404 1106 74-75 0.5127612083047026 1106 76-77 0.38836540069607395 1106 78-79 0.25235862481871507 1106 80-81 0.3198521644096992 1106 82-83 0.46271605683456585 1106 84-85 0.4150651095982454 1106 86-87 0.5001592218488717 1106 88-89 0.6090164483552165 1106 90-91 0.5118464366540465 1106 92-93 0.587806442745503 1106 94-95 0.6107863122228849 1106 96-97 0.75677032265245 1106 98-99 1.025083553787109 1106 100-101 1.7787742277148624 1107 1 0.024513597489658423 1107 2 0.01321756603878299 1107 3 0.013959287233355155 1107 4 -0.026909653027097136 1107 5 -0.0387864100746782 1107 6 -0.03399155446847146 1107 7 0.08125342330009744 1107 8 0.032642725355643165 1107 9 0.06139731128248371 1107 10-11 0.014336525819082624 1107 12-13 0.06537837674303404 1107 14-15 0.09848833731286533 1107 16-17 0.07208778174557295 1107 18-19 0.09657855314859631 1107 20-21 0.031148468268149543 1107 22-23 0.0420688115388117 1107 24-25 0.07666806723003816 1107 26-27 0.06282654420517275 1107 28-29 0.0033738288627205293 1107 30-31 0.018531922257501776 1107 32-33 -0.03392863546947211 1107 34-35 -0.0032497345732451777 1107 36-37 0.0463520936730788 1107 38-39 0.07524921520833772 1107 40-41 -0.03231511173421353 1107 42-43 -0.10138142070658063 1107 44-45 -0.06480400081670723 1107 46-47 -0.1261741911713088 1107 48-49 -0.05749903608498386 1107 50-51 -0.09941788746313307 1107 52-53 -0.13419475895699406 1107 54-55 -0.03953881958283745 1107 56-57 -0.048240564831864674 1107 58-59 0.09261131614050555 1107 60-61 0.07897787463562622 1107 62-63 0.16032875179480754 1107 64-65 0.03255031589583268 1107 66-67 -0.033520500460646474 1107 68-69 -0.1383228681059947 1107 70-71 -0.22254587854969543 1107 72-73 -0.14481791607622085 1107 74-75 -0.15228034863739737 1107 76-77 -0.12859080471999462 1107 78-79 -0.02086722400155594 1107 80-81 0.01376739808132399 1107 82-83 -0.0017252770997799871 1107 84-85 -0.09085056142439996 1107 86-87 -0.014809993384556464 1107 88-89 -0.10197644847292509 1107 90-91 0.12559500589389927 1107 92-93 0.22409431942352143 1107 94-95 0.31384835383380505 1107 96-97 0.3646629612303194 1107 98-99 0.6131256309431201 1107 100-101 1.336108126303749 1108 1 0.16979348251358317 1108 2 0.04397945212926402 1108 3 0.06962812441760491 1108 4 0.011662055615715872 1108 5 0.0019126651980343468 1108 6 -0.022149363218488816 1108 7 0.006504010577003783 1108 8 0.08480536102545955 1108 9 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-1.158013240209364 2316 88-89 -1.5812964562170642 2316 90-91 -2.0352595954096557 2316 92-93 -1.8229046914994882 2316 94-95 -1.7688631704509135 2316 96-97 -1.9128601885613143 2316 98-99 -1.771757228541329 2316 100-101 -1.81516456358845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 11.0 4 27.0 5 89.0 6 204.0 7 540.0 8 1254.0 9 2295.0 10 3777.0 11 5800.0 12 7991.0 13 11038.0 14 13433.0 15 17505.0 16 21817.0 17 28702.0 18 27939.0 19 21481.0 20 20414.0 21 22251.0 22 23826.0 23 26768.0 24 31360.0 25 37235.0 26 45117.0 27 55235.0 28 67433.0 29 81978.0 30 100155.0 31 121527.0 32 149531.0 33 188856.0 34 252869.0 35 353174.0 36 538894.0 37 861523.0 38 770924.0 39 81551.0 40 5.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.149030963741595 19.700211661056247 14.782247571920218 23.36850980328194 2 15.613081372425075 20.628608836823307 39.81326987997351 23.945039910778103 3 19.429220172060077 24.401062952258524 29.84825517863781 26.321461697043585 4 13.200835641695466 16.974266091046893 36.829142816372865 32.99575545088477 5 13.666254478105675 37.497164012482855 34.503838662485684 14.33274284692579 6 32.19362021351408 36.21718121658914 17.389583459030238 14.199615110866546 7 28.483690842613722 32.5518239294436 20.713725126954703 18.250760100987975 8 25.878306235894794 35.81413105655602 19.64731814842028 18.66024455912891 9 26.054421853857836 16.9673065826185 20.371983222077166 36.6062883414465 10-11 24.9458447604039 26.734976661864273 27.36080670215682 20.958371875575004 12-13 25.60717330052184 23.980701132922853 27.814163601019892 22.59796196553541 14-15 22.596685221183442 25.51574839123953 26.02792315000369 25.859643237573334 16-17 22.166430482935127 28.667439328152522 26.83275425446783 22.333375934444526 18-19 21.995964486479654 27.438800761540456 28.874574887940653 21.690659864039244 20-21 22.827037439902266 26.638307087057694 28.841404568992385 21.693250904047655 22-23 22.426740534254925 26.56619606537432 28.643083612986242 22.363979787384515 24-25 22.334231272179615 26.6917902620804 28.864342473316874 22.109635992423115 26-27 22.226007991254956 26.89550096676886 28.69123641895345 22.18725462302273 28-29 22.08155642646766 27.001903851136618 28.798921526535633 22.117618195860096 30-31 22.25659557370257 26.80539787484651 28.74476002838879 22.193246523062133 32-33 22.070879338896766 26.856692956752166 28.625910149742083 22.446517554608985 34-35 22.529100207209922 26.927050414989417 28.467048389582196 22.076800988218466 36-37 22.47531520298058 26.725068887077775 28.53852981643049 22.261086093511157 38-39 22.342805694332956 26.93805417418001 28.60154691805671 22.117593213430325 40-41 22.5173733728541 26.773233201149573 28.646530570551654 22.062862855444678 42-43 22.21652512738035 26.886013441610963 28.439939177392638 22.45752225361605 44-45 22.37181100016398 27.079232274224257 28.302371197640696 22.246585527971067 46-47 22.433753634040226 27.11805745920475 27.870062518134215 22.578126388620813 48-49 22.49054005845574 27.169765738926895 27.873024615249815 22.46666958736755 50-51 22.469589385196198 27.3015040926546 27.83921380576385 22.38969271638535 52-53 22.617426058352226 27.284327212053466 27.6816575646477 22.416589164946608 54-55 22.37322992538556 27.153335744961556 27.84655785967578 22.626876469977105 56-57 22.355469959676864 27.49812804216618 27.623538577352104 22.522863420804857 58-59 22.43339063682479 27.155884272482233 27.774882144817862 22.635842945875112 60-61 22.400464445637493 27.19096133512599 27.932529707860137 22.47604451137638 62-63 22.387623094131335 27.122616475036697 27.923804807805926 22.56595562302604 64-65 22.265270506982006 27.106224813686687 27.8703916729107 22.758113006420608 66-67 22.15773088636782 27.31693761289426 27.65302609821159 22.872305402526326 68-69 22.22698290447764 27.168445914289446 27.774400238526454 22.830170942706463 70-71 22.194706841236904 27.04175593971111 27.689190740295455 23.07434647875653 72-73 22.212222448920837 27.05304872782439 27.626419595772724 23.108309227482046 74-75 22.104221598728756 27.203047677222592 27.521196239595895 23.171534484452753 76-77 22.14309036891603 26.996588172037487 27.72134955637219 23.138971902674292 78-79 22.144068836596194 27.068145788397715 27.64292895986358 23.144856415142517 80-81 22.035939251826985 27.16983555224377 27.527390539544406 23.266834656384837 82-83 22.21730434780432 27.09192066389302 27.47395856151172 23.216816426790945 84-85 22.087902596923044 26.991402002986582 27.44094118589523 23.479754214195143 86-87 22.038728526651425 26.90897808826894 27.634473842029934 23.4178195430497 88-89 22.10956294988633 26.84235366120372 27.502238243583964 23.54584514532598 90-91 21.95381011798116 26.904625434985068 27.587921618677925 23.55364282835584 92-93 21.937854540807265 26.843130287014784 27.551413932428098 23.667601239749853 94-95 21.811742322303655 26.74674711010696 27.53353652812287 23.90797403946652 96-97 21.843924296966637 26.5119459000115 27.602675108545327 24.04145469447653 98-99 21.678668480811908 26.538620890792046 27.4925867466426 24.290123881753445 100-101 21.87452082401059 26.010095162395675 28.01794973698128 24.097434276612457 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 531.0 1 352.5 2 251.0 3 349.5 4 329.5 5 343.0 6 588.0 7 1209.5 8 1984.5 9 2438.5 10 2367.5 11 2280.0 12 2500.0 13 2804.5 14 3143.0 15 3421.0 16 3694.5 17 4073.0 18 4415.5 19 4836.5 20 5609.0 21 6612.0 22 8066.5 23 10170.5 24 12824.5 25 16343.0 26 21547.0 27 27853.5 28 33331.0 29 39381.0 30 46395.5 31 54420.0 32 62883.0 33 70047.0 34 77825.5 35 85896.0 36 95431.0 37 104839.0 38 110814.5 39 115705.0 40 120024.0 41 125089.0 42 129782.5 43 134609.5 44 140440.0 45 145507.5 46 148163.5 47 150545.0 48 161802.0 49 163503.0 50 153840.5 51 148948.5 52 142674.0 53 138322.5 54 135188.5 55 125579.5 56 113273.5 57 100991.5 58 90391.0 59 80429.0 60 67675.0 61 54731.5 62 43926.5 63 33846.0 64 24581.5 65 18357.0 66 13495.5 67 10246.5 68 7676.5 69 5512.0 70 4227.0 71 3162.0 72 2459.0 73 2156.0 74 1753.5 75 1324.5 76 955.0 77 585.0 78 385.5 79 258.5 80 166.0 81 113.0 82 72.5 83 47.5 84 30.0 85 15.5 86 11.0 87 8.0 88 4.0 89 3.0 90 2.0 91 0.5 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23507116598052089 2 0.0 3 0.0 4 0.0 5 0.030466625026442377 6 0.0034547200112153226 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0029164846471491676 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.1306434916704943E-4 26-27 0.0010889878296222212 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0069219571239205555 36-37 0.017023258026278403 38-39 0.013243093376325404 40-41 0.00871190263697777 42-43 0.00742264118351698 44-45 0.0035047884171749656 46-47 3.504788417174965E-4 48-49 0.001502052178789271 50-51 1.251710148991059E-5 52-53 0.0 54-55 0.0 56-57 0.0013518469609103438 58-59 3.504788417174965E-4 60-61 0.002390766384572923 62-63 7.760602923744566E-4 64-65 0.0013518469609103438 66-67 0.0050068405959642365 68-69 2.3782492830830122E-4 70-71 3.755130446973177E-5 72-73 0.0 74-75 4.380985521468707E-4 76-77 0.05991936483220199 78-79 0.143358363363946 80-81 0.0011140220326020425 82-83 9.012313072735624E-4 84-85 0.0 86-87 0.10759700440727143 88-89 0.3578263802920741 90-91 0.39570312940054353 92-93 4.88166958106513E-4 94-95 2.5034202979821183E-4 96-97 1.376881163890165E-4 98-99 0.0 100-101 0.001189124641541506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3994535.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.15867411023677 #Duplication Level Percentage of deduplicated Percentage of total 1 71.08924081557242 36.36831303628012 2 15.703034888020673 16.06692888755856 3 5.677554245970693 8.713684422384173 4 2.6333129682024814 5.388671998821241 5 1.3832165679603234 3.5381762812081177 6 0.8337616523368433 2.5592484394507857 7 0.5199630096405872 1.8620432711706492 8 0.3873079716037824 1.5851329839659793 9 0.26293590696253816 1.2106307138558423 >10 1.3532620936472652 12.42076617051309 >50 0.1007991076816928 3.5724030507462285 >100 0.052234321517671746 4.849366734478276 >500 0.002887110210963815 0.979806573483504 >1k 3.425384705253964E-4 0.31259980745150917 >5k 1.468022016537412E-4 0.5722276286319046 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9433 0.2361476367086532 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8311 0.20805926096529384 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5114 0.1280249140388055 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 2.503420297982118E-5 9 0.0 0.0 0.0 0.0 5.006840595964236E-5 10-11 0.0 0.0 0.0 2.503420297982118E-5 5.006840595964236E-5 12-13 3.755130446973177E-5 0.0 0.0 3.755130446973177E-5 6.258550744955296E-5 14-15 5.006840595964236E-5 0.0 0.0 6.258550744955296E-5 1.0013681191928473E-4 16-17 6.258550744955296E-5 0.0 0.0 7.510260893946354E-5 1.2517101489910591E-4 18-19 7.510260893946354E-5 0.0 0.0 7.510260893946354E-5 1.2517101489910591E-4 20-21 7.510260893946354E-5 0.0 0.0 2.0027362383856945E-4 1.2517101489910591E-4 22-23 7.510260893946354E-5 0.0 0.0 3.880301461872283E-4 1.6272231936883768E-4 24-25 1.0013681191928473E-4 0.0 0.0 5.50752465556066E-4 2.1279072532848003E-4 26-27 1.0013681191928473E-4 0.0 0.0 9.262655102533837E-4 2.2530782681839064E-4 28-29 1.6272231936883766E-4 0.0 0.0 0.0033170318948263066 2.2530782681839064E-4 30-31 1.7523942085874826E-4 0.0 0.0 0.011277908442409442 2.2530782681839064E-4 32-33 1.7523942085874826E-4 0.0 0.0 0.027500071973333567 2.5034202979821183E-4 34-35 2.1279072532848003E-4 0.0 0.0 0.05313509582467046 2.75376232778033E-4 36-37 2.75376232778033E-4 0.0 0.0 0.09767094292577233 2.75376232778033E-4 38-39 3.0041043575785415E-4 0.0 0.0 0.1606194463185327 2.75376232778033E-4 40-41 3.129275372477648E-4 0.0 0.0 0.22921316248324272 2.75376232778033E-4 42-43 3.3796174022758594E-4 0.0 0.0 0.29919627691333284 2.8789333426794357E-4 44-45 3.755130446973177E-4 0.0 0.0 0.3888938261900321 3.0041043575785415E-4 46-47 4.7564985661660244E-4 0.0 0.0 0.4968788607434908 3.2544463873767536E-4 48-49 4.7564985661660244E-4 0.0 0.0 0.613050079671351 3.504788417174965E-4 50-51 4.7564985661660244E-4 0.0 0.0 0.7369318331169961 3.504788417174965E-4 52-53 4.7564985661660244E-4 0.0 0.0 0.8606007958373127 3.629959432074071E-4 54-55 4.7564985661660244E-4 0.0 0.0 1.0035085435476219 3.8803014618722827E-4 56-57 4.7564985661660244E-4 0.0 0.0 1.1733781278671986 4.005472476771389E-4 58-59 4.7564985661660244E-4 0.0 0.0 1.3598829400668664 4.005472476771389E-4 60-61 4.7564985661660244E-4 0.0 0.0 1.5636363181196309 4.005472476771389E-4 62-63 4.7564985661660244E-4 0.0 0.0 1.7688041286407554 4.005472476771389E-4 64-65 4.7564985661660244E-4 0.0 0.0 1.9824209826675696 4.005472476771389E-4 66-67 5.257182625762448E-4 0.0 0.0 2.2346030263847982 4.130643491670495E-4 68-69 5.257182625762448E-4 0.0 0.0 2.520781517748624 4.2558145065696006E-4 70-71 5.50752465556066E-4 0.0 0.0 2.827513089758883 4.506156536367813E-4 72-73 5.50752465556066E-4 0.0 0.0 3.1396896009172535 5.006840595964237E-4 74-75 5.50752465556066E-4 0.0 0.0 3.459176099345731 5.006840595964237E-4 76-77 5.50752465556066E-4 0.0 0.0 3.809742560773657 5.006840595964237E-4 78-79 5.50752465556066E-4 0.0 0.0 4.205358070463771 5.006840595964237E-4 80-81 6.258550744955296E-4 0.0 0.0 4.633843488666391 5.006840595964237E-4 82-83 6.383721759854401E-4 0.0 0.0 5.070965706897049 5.006840595964237E-4 84-85 6.634063789652614E-4 0.0 0.0 5.511329854413592 5.006840595964237E-4 86-87 6.759234804551719E-4 0.0 0.0 5.975013362005841 5.006840595964237E-4 88-89 6.759234804551719E-4 0.0 0.0 6.475246805948627 5.006840595964237E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12280 0.0 30.606632 1 GTATCAA 17445 0.0 26.562857 1 GTACATG 15780 0.0 23.034203 1 ATCAACG 20275 0.0 22.567003 3 TACATGG 15690 0.0 22.506487 2 TCAACGC 20500 0.0 22.296165 4 CAACGCA 20875 0.0 21.872898 5 TATCAAC 21045 0.0 21.854082 2 ACATGGG 15605 0.0 21.473295 3 AACGCAG 21435 0.0 21.316393 6 GAGTACT 11555 0.0 20.428387 12-13 ACGCAGA 23835 0.0 19.128748 7 GTACTTT 12385 0.0 18.982725 14-15 CGCAGAG 23985 0.0 18.969557 8 AGTACTT 12425 0.0 18.329718 12-13 GTGTACG 470 0.0 18.22055 1 TACCTGG 3080 0.0 17.567577 2 GCAGAGT 26150 0.0 17.16318 9 CATGGGA 7705 0.0 16.570566 4 AGAGTAC 22430 0.0 16.56317 10-11 >>END_MODULE