FastQCFastQC Report
Thu 26 May 2016
SRR938715_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938715_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616184
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115100.712171386426298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91590.5667052761319256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63110.3904877167451231No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43280.2677912910906184No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38780.2399479267212149No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25460.1575315681877806No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23720.1467654672982779No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23440.1450329912930706No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20700.12807947609925602No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17970.11118783504848458No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18200.042.4285962
GTACCTG22250.034.939121
TATCACG1201.5419027E-731.5828382
ACCTGGG25400.030.0285663
GTATCAA141700.028.769131
GGTATCA97100.025.9711111
GTACATG105050.024.637391
TACATGG104700.024.3432222
TATCAAC171000.023.5486092
ATCAACG170950.023.5277843
TCAACGC171500.023.5075764
ACATGGG105500.023.2156333
CAACGCA174150.023.1226635
AACGCAG177800.022.7369586
AGTACTT93350.022.41789412-13
GAGTACT91850.021.85614812-13
ATCACGC1754.1450858E-621.6568053
ACGCAGA191450.020.9941797
CATGGGG33400.020.9921884
CGCAGAG192100.020.971788
Produced by FastQC (version 0.11.5)