##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938715_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1616184 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66924124975869 33.0 31.0 34.0 30.0 34.0 2 31.714725551051117 33.0 31.0 34.0 30.0 34.0 3 31.712272241279457 33.0 31.0 34.0 30.0 34.0 4 35.29462734441128 37.0 35.0 37.0 33.0 37.0 5 35.33798998133876 37.0 35.0 37.0 33.0 37.0 6 35.43727385000718 37.0 35.0 37.0 33.0 37.0 7 35.3641831623132 37.0 35.0 37.0 33.0 37.0 8 35.33502311617984 37.0 35.0 37.0 33.0 37.0 9 36.91439588561698 39.0 37.0 39.0 33.0 39.0 10-11 36.803947137207146 39.0 37.0 39.0 33.0 39.0 12-13 36.6818679061295 39.0 37.0 39.0 32.0 39.0 14-15 37.81604910084495 40.0 38.0 41.0 32.0 41.0 16-17 37.75369574256396 40.0 37.5 41.0 32.0 41.0 18-19 37.72507152650935 40.0 37.5 41.0 32.0 41.0 20-21 37.6347250684328 40.0 37.0 41.0 32.0 41.0 22-23 37.52721379496394 40.0 37.0 41.0 32.0 41.0 24-25 37.53606303490197 40.0 37.0 41.0 32.0 41.0 26-27 37.435457534538145 40.0 37.0 41.0 32.0 41.0 28-29 37.30426455156096 40.0 37.0 41.0 31.5 41.0 30-31 37.05874980819016 39.5 36.0 41.0 31.0 41.0 32-33 36.80927481029388 39.0 35.5 41.0 30.5 41.0 34-35 36.4606647510432 39.0 35.0 41.0 30.0 41.0 36-37 36.19726838033293 39.0 35.0 41.0 30.0 41.0 38-39 36.01376668745638 39.0 35.0 41.0 29.5 41.0 40-41 35.824416031837956 39.0 35.0 41.0 28.5 41.0 42-43 35.54193303485246 39.0 35.0 40.0 27.0 41.0 44-45 35.41265845968033 39.0 35.0 41.0 26.0 41.0 46-47 35.14477466674587 39.0 35.0 41.0 24.5 41.0 48-49 34.867130846487775 39.0 35.0 41.0 24.0 41.0 50-51 33.728439645485906 38.0 33.0 40.0 21.5 40.5 52-53 33.83510324319508 38.0 33.5 39.5 22.0 40.5 54-55 34.35226403676809 38.0 34.0 40.0 22.5 41.0 56-57 34.27955016260525 38.0 34.0 40.0 22.0 41.0 58-59 34.07694668428842 38.0 34.0 40.0 21.5 41.0 60-61 33.805291043594046 38.0 34.0 40.0 20.0 41.0 62-63 33.451795092637965 37.0 33.0 40.0 18.5 41.0 64-65 33.070646040302336 37.0 33.0 40.0 16.5 41.0 66-67 32.68979089014617 36.0 32.5 39.0 13.0 41.0 68-69 32.30408511654613 36.0 32.0 39.0 9.5 41.0 70-71 31.912642063032425 35.0 31.5 39.0 8.0 40.0 72-73 31.50388507744168 35.0 31.0 38.0 7.0 40.0 74-75 31.05952601931463 35.0 31.0 37.0 2.0 39.5 76-77 30.629377286249586 35.0 31.0 37.0 2.0 39.0 78-79 30.21808717324265 35.0 30.0 36.0 2.0 39.0 80-81 29.78939805121199 34.5 30.0 36.0 2.0 37.5 82-83 29.342798220994638 34.0 29.0 35.0 2.0 37.0 84-85 28.94892320428862 34.0 29.0 35.0 2.0 37.0 86-87 28.56429001895824 34.0 29.0 35.0 2.0 36.0 88-89 28.199886275325085 34.0 28.5 35.0 2.0 36.0 90-91 27.804370356345565 34.0 27.0 35.0 2.0 35.5 92-93 27.379465766274137 34.0 26.5 35.0 2.0 35.0 94-95 26.90811937254669 34.0 25.5 35.0 2.0 35.0 96-97 26.347999980200274 33.0 25.0 35.0 2.0 35.0 98-99 25.681353422630096 33.0 22.0 35.0 2.0 35.0 100-101 23.467442135301425 31.0 8.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21097776052445738 1101 2 0.07898597089328163 1101 3 0.2752317177352772 1101 4 0.2341701414952695 1101 5 -0.08307487454086271 1101 6 -0.027374349531761766 1101 7 0.006491308356892489 1101 8 0.12722735900187843 1101 9 0.19659825572082212 1101 10-11 0.23760100254312988 1101 12-13 0.23398085154443748 1101 14-15 0.4396022415654173 1101 16-17 0.4125086195126286 1101 18-19 0.39328100917516196 1101 20-21 0.3992802444765218 1101 22-23 0.31517263856049027 1101 24-25 0.37016149012497834 1101 26-27 0.3125361604032406 1101 28-29 0.3434407260603862 1101 30-31 0.2913184959700672 1101 32-33 0.45822221313176215 1101 34-35 0.4334218306109605 1101 36-37 0.47451854734816834 1101 38-39 0.3796197900581646 1101 40-41 0.4749441540345174 1101 42-43 0.37813651799475423 1101 44-45 0.4686217271204569 1101 46-47 0.5761233015431628 1101 48-49 0.5526618849107834 1101 50-51 0.4629679494669645 1101 52-53 0.4491079761979577 1101 54-55 0.5405973371257957 1101 56-57 0.4336012429664038 1101 58-59 0.49342994743884105 1101 60-61 0.4844968816710633 1101 62-63 0.3303030038991679 1101 64-65 0.4132569243318116 1101 66-67 0.2887664019573819 1101 68-69 0.4489198776377066 1101 70-71 0.25352175474644056 1101 72-73 0.17718691293347888 1101 74-75 0.17422632969352847 1101 76-77 0.23692414804831685 1101 78-79 0.2801532288779214 1101 80-81 0.016769868184749015 1101 82-83 -0.028288652582507012 1101 84-85 0.2272709765920098 1101 86-87 0.2640383197296501 1101 88-89 0.06917847279058265 1101 90-91 0.07316085313368248 1101 92-93 0.021827718366218107 1101 94-95 0.10417233562381512 1101 96-97 -0.18651800982139477 1101 98-99 -0.27593362065445604 1101 100-101 -0.056815165226648645 1102 1 0.1830265476212105 1102 2 0.25834074325662115 1102 3 0.23658302629309702 1102 4 0.179401777368021 1102 5 0.16949645422521087 1102 6 0.28343404587094057 1102 7 0.27666364982086833 1102 8 0.2109259263053147 1102 9 0.3326897380565583 1102 10-11 0.18480959480490355 1102 12-13 0.2583435896810684 1102 14-15 0.17793148591061936 1102 16-17 0.3137383357646044 1102 18-19 0.31073588747675984 1102 20-21 0.2712865501695987 1102 22-23 0.28574049372706156 1102 24-25 0.3347790921986942 1102 26-27 0.38840280552314255 1102 28-29 0.3335314591563687 1102 30-31 0.3818671386055641 1102 32-33 0.4249383802236508 1102 34-35 0.30487293986120534 1102 36-37 0.28085578521627497 1102 38-39 0.29731604924614174 1102 40-41 0.42807790230629195 1102 42-43 0.26813192298413213 1102 44-45 0.3831204547187568 1102 46-47 0.2062178053228223 1102 48-49 0.38304620898716735 1102 50-51 0.10772261834053865 1102 52-53 0.24916645444144336 1102 54-55 0.21923735273087885 1102 56-57 0.18382650458818972 1102 58-59 0.1735545677179431 1102 60-61 0.03233253372999911 1102 62-63 0.21717902512600062 1102 64-65 0.3422893114030998 1102 66-67 0.39436778519432636 1102 68-69 0.20578301161450696 1102 70-71 0.1440250185914671 1102 72-73 0.061999260208544626 1102 74-75 0.032199081198946544 1102 76-77 0.10179614884489752 1102 78-79 -0.02519930556017158 1102 80-81 -0.05714185700913177 1102 82-83 -0.15290158619072614 1102 84-85 -0.08792491940241476 1102 86-87 -0.1998522831063596 1102 88-89 -0.05073722549596127 1102 90-91 -0.19888438364403171 1102 92-93 -0.45807053846793977 1102 94-95 -0.46806421029935663 1102 96-97 -0.47064024853015596 1102 98-99 -0.5712363037162476 1102 100-101 -0.3500727420971792 1103 1 -0.023742933318825266 1103 2 0.01672348098058407 1103 3 0.17227196097169184 1103 4 0.18068951318887372 1103 5 0.252127564344363 1103 6 0.28502508313397357 1103 7 0.24871328603848752 1103 8 0.16479939659592446 1103 9 0.3374006750427938 1103 10-11 0.20688364260377767 1103 12-13 0.2079321798076279 1103 14-15 0.41452215450895125 1103 16-17 0.25054243133046583 1103 18-19 0.3529430132671436 1103 20-21 0.3350942387216662 1103 22-23 0.4890911576224326 1103 24-25 0.22795540758436772 1103 26-27 0.43148316844862933 1103 28-29 0.24059447690608948 1103 30-31 0.35006612188811204 1103 32-33 0.5031799891162407 1103 34-35 0.42848740825667875 1103 36-37 0.4799169855486696 1103 38-39 0.30982055791573515 1103 40-41 0.4759805987398096 1103 42-43 0.6257691609083693 1103 44-45 0.5158521006970247 1103 46-47 0.4474034309866681 1103 48-49 0.45085819397753113 1103 50-51 0.5375948212217025 1103 52-53 0.48188355311006603 1103 54-55 0.34186140840123613 1103 56-57 0.3110044660119158 1103 58-59 0.3388572414337432 1103 60-61 0.2855294544830258 1103 62-63 0.2598787666621547 1103 64-65 0.3026369323201408 1103 66-67 0.2649210413007026 1103 68-69 0.32942876668962384 1103 70-71 0.5193472016444396 1103 72-73 0.43888757074777374 1103 74-75 0.29452055282870404 1103 76-77 0.331451736307109 1103 78-79 0.44268043402306034 1103 80-81 0.36181567879618015 1103 82-83 0.3651641796055607 1103 84-85 0.2141328274385259 1103 86-87 0.13534005026217955 1103 88-89 0.031727686592457616 1103 90-91 0.0804801579861838 1103 92-93 0.07895037179508435 1103 94-95 0.02128875326660662 1103 96-97 -0.09351798536733114 1103 98-99 -0.11740587751993203 1103 100-101 -0.003807315472410977 1104 1 0.16852736805212487 1104 2 0.13577988635327998 1104 3 0.08012665216070758 1104 4 0.2376682644536814 1104 5 0.11125499723525678 1104 6 0.06445686298835085 1104 7 -0.019282666583379182 1104 8 0.02105993664314809 1104 9 0.09000667071765633 1104 10-11 -0.01993519754462625 1104 12-13 -0.007793074641512021 1104 14-15 0.07069043814286857 1104 16-17 0.22752495221215696 1104 18-19 0.14730446000642416 1104 20-21 0.16026337547939562 1104 22-23 0.12761961822993584 1104 24-25 0.11820400389807872 1104 26-27 0.15034514095026452 1104 28-29 0.10235667360072398 1104 30-31 0.19276893719672472 1104 32-33 0.12386625779073057 1104 34-35 0.18254669044226546 1104 36-37 0.24251742599864912 1104 38-39 0.2332397988339423 1104 40-41 0.22335476297607215 1104 42-43 0.3456429779430792 1104 44-45 0.2883586975265757 1104 46-47 0.13953683574432318 1104 48-49 0.1397675842915973 1104 50-51 0.27798428216649285 1104 52-53 0.2567124323434484 1104 54-55 0.3028895218071881 1104 56-57 0.1497194234549184 1104 58-59 0.18692977679869927 1104 60-61 0.22013751352713484 1104 62-63 0.1469757648567125 1104 64-65 0.1523700831246515 1104 66-67 0.190527652018325 1104 68-69 0.24535837405426264 1104 70-71 0.07427647172757545 1104 72-73 -2.4105254846062962E-4 1104 74-75 -0.06734282831924432 1104 76-77 -0.060930661566032995 1104 78-79 -0.007549711007989401 1104 80-81 -0.049332467147511494 1104 82-83 -0.09144256999267597 1104 84-85 -0.08011652279856207 1104 86-87 -0.08068280552021179 1104 88-89 -0.1274830746717548 1104 90-91 -0.22229646245307677 1104 92-93 -0.19258406067917022 1104 94-95 -0.19231857169421218 1104 96-97 -0.31602656620577463 1104 98-99 -0.25999461231746324 1104 100-101 0.0624476693499183 1105 1 0.16058050388942746 1105 2 0.09535197828298081 1105 3 0.1286307372720934 1105 4 0.16286076430673546 1105 5 -0.05375999130291831 1105 6 0.02595101451952786 1105 7 0.11571997845276627 1105 8 0.10300315653381631 1105 9 0.06361508781046865 1105 10-11 0.17911552837084344 1105 12-13 0.10356294722411263 1105 14-15 0.32619211333242504 1105 16-17 0.20107752965293457 1105 18-19 0.26019460283837503 1105 20-21 0.23981430711990726 1105 22-23 0.3367802600920413 1105 24-25 0.25535794500193987 1105 26-27 0.2302134769286326 1105 28-29 0.26290448997644233 1105 30-31 0.23641497258142863 1105 32-33 0.21194302261762488 1105 34-35 0.14706499997027578 1105 36-37 0.03670128540028372 1105 38-39 0.2424974253548342 1105 40-41 0.11012370437381946 1105 42-43 0.2746149405598928 1105 44-45 0.11594054670074172 1105 46-47 0.039595614325406814 1105 48-49 0.04927795871116558 1105 50-51 0.20200414932689625 1105 52-53 0.044133815991244774 1105 54-55 0.07091985155503266 1105 56-57 0.07563196092626612 1105 58-59 0.10224747502946485 1105 60-61 0.1908922305082683 1105 62-63 0.03117902119009841 1105 64-65 0.016532782237099752 1105 66-67 -0.06413936731983227 1105 68-69 -0.013367306307813465 1105 70-71 0.028911162846132044 1105 72-73 0.04022212267049241 1105 74-75 -0.18083839053637263 1105 76-77 -0.0726305381310155 1105 78-79 -0.20153372323393626 1105 80-81 0.012655424464782072 1105 82-83 -0.026436398241663994 1105 84-85 -0.24469948876576098 1105 86-87 -0.26678167109359663 1105 88-89 -0.22907009636105258 1105 90-91 -0.166659596527019 1105 92-93 -0.14999603975399722 1105 94-95 -0.03138575561239065 1105 96-97 0.2475072020890643 1105 98-99 0.17239826371850597 1105 100-101 0.47898502313820046 1106 1 0.04265180538646263 1106 2 0.1164359505951893 1106 3 0.1952507914979016 1106 4 0.1606556074170271 1106 5 0.17148414135608903 1106 6 0.13597696211160581 1106 7 0.11041431737182705 1106 8 0.2516611066740424 1106 9 0.25126939441156537 1106 10-11 0.28852988189513695 1106 12-13 0.1620394195945991 1106 14-15 0.3209864747808311 1106 16-17 0.28958756298083443 1106 18-19 0.3917409317202214 1106 20-21 0.33887972768065566 1106 22-23 0.26533115699309207 1106 24-25 0.20225841190322313 1106 26-27 0.05858044890678826 1106 28-29 0.09431467168727181 1106 30-31 0.08479829775757963 1106 32-33 0.2016752729885809 1106 34-35 0.19546133778616337 1106 36-37 0.3791263753234517 1106 38-39 0.24922146416746216 1106 40-41 0.1590446263679013 1106 42-43 0.34713923729469087 1106 44-45 0.45293742366720124 1106 46-47 0.431237615242118 1106 48-49 0.4572737402852667 1106 50-51 0.5018299353291837 1106 52-53 0.3994122864816845 1106 54-55 0.20896356625581092 1106 56-57 0.25883225458463954 1106 58-59 0.28876638025860046 1106 60-61 0.3860293876155012 1106 62-63 0.24406833218053947 1106 64-65 0.1512576911486221 1106 66-67 0.062499059976410365 1106 68-69 0.14641547971777413 1106 70-71 0.159174290074791 1106 72-73 0.02559882888004239 1106 74-75 0.009870584689114281 1106 76-77 0.03246517274755334 1106 78-79 0.3686214961941303 1106 80-81 0.020321228801289948 1106 82-83 0.026437258737384894 1106 84-85 0.18498409044819653 1106 86-87 0.11151829550081871 1106 88-89 0.3254612155460954 1106 90-91 0.4740111176532622 1106 92-93 0.4517614434849442 1106 94-95 0.43175293193665 1106 96-97 0.3872196421726528 1106 98-99 0.5781545117277673 1106 100-101 0.7401531697498633 1107 1 0.04470337590699813 1107 2 0.10825227640635404 1107 3 0.015607704991669635 1107 4 0.10497236622682493 1107 5 0.1506561406755509 1107 6 0.09688178364719846 1107 7 -0.10663640247882711 1107 8 0.10310042478932502 1107 9 0.0501269677492715 1107 10-11 0.027908578668871087 1107 12-13 0.09709843769163484 1107 14-15 0.056798488998516916 1107 16-17 0.02108855849450464 1107 18-19 0.10929917961615132 1107 20-21 0.07858470616867663 1107 22-23 0.17562227002182595 1107 24-25 0.10832433958555043 1107 26-27 -0.061374542226317885 1107 28-29 -0.16629187893065733 1107 30-31 -0.015736704625822995 1107 32-33 0.08030709693209559 1107 34-35 -0.09836214744665739 1107 36-37 0.06036657537648438 1107 38-39 0.1034206695958062 1107 40-41 0.09701891314634281 1107 42-43 0.08362699761116943 1107 44-45 0.1058324985184882 1107 46-47 0.1174917862068412 1107 48-49 0.09915187377759338 1107 50-51 0.20154174216734333 1107 52-53 0.05454151933071927 1107 54-55 0.007129682924805536 1107 56-57 -0.10400661474479733 1107 58-59 -0.08667638958385027 1107 60-61 -0.09152940440260693 1107 62-63 -0.05851858558957446 1107 64-65 0.027182698850630516 1107 66-67 0.08532785921372721 1107 68-69 -0.07877293216584746 1107 70-71 0.07825029398020789 1107 72-73 -0.13923283044735868 1107 74-75 -0.11552696936674423 1107 76-77 -0.03459577813277548 1107 78-79 -0.06885848240884229 1107 80-81 -0.27468993719888957 1107 82-83 -0.15600345876817912 1107 84-85 -0.12925642994719055 1107 86-87 -0.06545974188880166 1107 88-89 -0.08112967508227698 1107 90-91 0.16063716930348804 1107 92-93 0.06277712033461569 1107 94-95 0.085247307552617 1107 96-97 -0.08757121565512094 1107 98-99 0.01300890923124598 1107 100-101 0.2979146127925354 1108 1 0.04805905648947473 1108 2 -0.048262687425143724 1108 3 -0.06716701015182025 1108 4 0.023009338474004437 1108 5 0.03126660826573868 1108 6 -0.045822769329021185 1108 7 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70-71 -2.0661829584846743 2316 72-73 -2.1939635301287375 2316 74-75 -2.1487331189988517 2316 76-77 -2.2338850292602075 2316 78-79 -2.2390235734146415 2316 80-81 -2.2052140952961885 2316 82-83 -1.9626987173360604 2316 84-85 -2.0792502697048008 2316 86-87 -1.9974635797372642 2316 88-89 -1.9133648099636673 2316 90-91 -2.026505584628513 2316 92-93 -2.321298984661045 2316 94-95 -2.3882369499741323 2316 96-97 -2.435005919703464 2316 98-99 -2.6140590911191737 2316 100-101 -2.954597212953228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5331.0 3 1545.0 4 1648.0 5 1766.0 6 2052.0 7 2437.0 8 3123.0 9 3907.0 10 4668.0 11 6379.0 12 8402.0 13 11205.0 14 11792.0 15 11741.0 16 15037.0 17 19082.0 18 16046.0 19 10817.0 20 10554.0 21 10671.0 22 11851.0 23 13336.0 24 14708.0 25 16733.0 26 19838.0 27 22844.0 28 27232.0 29 31949.0 30 38477.0 31 47070.0 32 58201.0 33 74670.0 34 100161.0 35 145868.0 36 200933.0 37 299254.0 38 285338.0 39 49481.0 40 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.084945327 19.1313670264023 14.206737994094262 21.576949652503433 2 15.185411343292454 21.12830967825041 41.185235462930734 22.501043515526394 3 19.079867701489874 27.1363664230295 30.0604458789237 23.723319996556917 4 12.316628907570937 17.218085719134262 37.81677692983249 32.64850844346231 5 12.789518514503415 38.174288767403326 34.03174799832742 15.00444471976584 6 29.378049542866446 36.47814975263229 19.094695443395718 15.04910526110555 7 27.19586669126085 33.98986604009265 21.571865572142876 17.242401696503624 8 25.902860096378532 34.365043747930216 20.761124230503775 18.97097192518748 9 27.163636228580906 17.07801557166892 21.318751245745894 34.43959695400427 10-11 24.27485310434582 27.340031909279844 27.620499313687407 20.764615672686926 12-13 25.901553797956122 25.151922578137135 28.102628290005143 20.843895333901592 14-15 22.893637698720358 25.94184126512503 27.548038755983086 23.616482280171528 16-17 21.071867727751464 29.254823457389634 28.142083352290122 21.53122546256878 18-19 20.71318141370372 27.41560722714435 31.44457770727744 20.426633651874493 20-21 22.083027940928854 26.406517734219463 31.21254816120757 20.297906163644107 22-23 21.54185857969244 26.65925378581624 30.91521115102001 20.88367648347131 24-25 21.179760792022314 26.782147732335492 31.341192584682727 20.696898890959467 26-27 21.06645654371481 26.85832645737967 31.29059973280534 20.78461726610018 28-29 20.712822502076513 26.84865485532818 31.657251794253742 20.78127084834156 30-31 20.87145803597381 26.975775084916748 31.445585954198663 20.70718092491078 32-33 20.657031272729974 27.153277170888547 31.124472509847205 21.065219046534274 34-35 21.263191141671147 27.01803951548374 30.94492212599644 20.77384721684867 36-37 21.383944124830894 26.79179301597681 30.861999359149877 20.962263500042422 38-39 21.102839346477083 26.96153354974084 31.069526841874513 20.866100261907565 40-41 21.146079911526968 26.904531136418708 31.118276764024284 20.831112188030048 42-43 20.991354477604986 27.14144636463583 30.71578039341446 21.151418764344722 44-45 21.070129450391335 27.4064394227796 30.336546156033485 21.18688497079558 46-47 21.22201963617175 27.381592044593933 29.811298707796574 21.585089611437745 48-49 21.414917642583045 27.482922110901065 29.650379601892013 21.451780644623877 50-51 21.38244474065409 27.81454154547469 29.49447289997703 21.308540813894187 52-53 21.477506116725014 27.926144275143223 29.38449211290579 21.21185749522597 54-55 21.090018230265724 27.78204234916211 29.60335963969586 21.52457978087631 56-57 21.30966614549802 28.19257222825695 29.17762446526357 21.320137160981453 58-59 21.314418938418452 27.681671541270525 29.369615090096033 21.634294430214986 60-61 21.318857889601293 27.629613480932424 29.684255703440353 21.367272926025922 62-63 21.44534863451211 27.581584518432045 29.633273227774175 21.33979361928167 64-65 21.186263389378148 27.518884777754742 29.672748817302036 21.622103015565077 66-67 21.06025390713033 27.92950457304516 29.362857292952476 21.64738422687203 68-69 21.093797604199178 27.88379632335477 29.384218504595495 21.638187567850554 70-71 20.97896510806251 27.91759139715417 29.285575945307972 21.81786754947535 72-73 21.152990729012053 27.75769538626526 29.13723082661776 21.952083058104925 74-75 21.09167134978143 27.990831870378013 29.03859591101916 21.878900868821397 76-77 21.214850311310833 27.737973706005103 29.228567566181713 21.81860841650235 78-79 21.1620753111348 27.85627757974611 29.1431330374095 21.838514071709593 80-81 20.964572219721028 28.226392535816007 28.96808196956125 21.840953274901715 82-83 21.193191405263153 28.17455841303076 28.714906797749855 21.91734338395623 84-85 21.138850789298527 27.905949150045956 28.736482241171547 22.21871781948397 86-87 21.30833715320131 27.791007901299793 28.92926122458493 21.97139372091397 88-89 21.408391344968607 27.588145672764025 28.904153527412014 22.099309454855355 90-91 21.208631564028064 27.842624952289306 29.040924897460858 21.907818586221772 92-93 21.31072688408403 27.86588434294723 28.894992094187185 21.928396678781557 94-95 21.11311566826665 27.79422799440741 28.741381250400206 22.351275086925735 96-97 21.40228766032366 27.513097256336945 28.63209127763448 22.452523805704914 98-99 21.36670658667745 27.478633262331044 28.58457193636711 22.570088214624402 100-101 21.642352541911446 27.183617249338575 28.9845438184131 22.189486390336878 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4178.0 1 2637.0 2 2603.0 3 3382.5 4 1982.5 5 1462.0 6 2248.0 7 4208.0 8 6176.0 9 6430.5 10 5030.5 11 3755.0 12 3581.0 13 3734.0 14 3864.5 15 3900.5 16 3956.5 17 4178.5 18 4365.0 19 4499.0 20 4713.5 21 5221.5 22 5989.5 23 6896.0 24 7830.0 25 9076.5 26 11245.0 27 13658.5 28 15790.0 29 18312.5 30 21216.0 31 24187.5 32 27904.5 33 31392.0 34 34806.0 35 37686.0 36 40812.5 37 44479.0 38 45961.0 39 46519.5 40 47475.0 41 49769.5 42 51662.5 43 52572.0 44 54358.5 45 55841.0 46 56021.0 47 56851.0 48 64633.5 49 65534.0 50 58383.0 51 54702.5 52 50512.0 53 48345.5 54 46364.0 55 42114.5 56 37788.0 57 33598.0 58 29934.0 59 26525.0 60 22665.0 61 18779.5 62 15181.0 63 11915.0 64 8895.0 65 6839.5 66 5130.5 67 3964.0 68 2952.0 69 1990.5 70 1504.5 71 1220.5 72 1049.0 73 876.0 74 682.5 75 538.5 76 389.5 77 239.0 78 152.0 79 121.0 80 84.5 81 57.5 82 45.0 83 30.5 84 20.5 85 13.0 86 14.0 87 12.0 88 11.0 89 9.0 90 5.0 91 5.0 92 4.5 93 4.5 94 4.5 95 4.0 96 4.5 97 2.0 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15462348346475402 2 0.08971750741252234 3 0.08359196725125358 4 0.09664741143335165 5 0.11811773906931389 6 0.035453883963707106 7 0.0387332135449924 8 0.042012543126277696 9 0.04244566212757953 10-11 0.041239116338238714 12-13 0.04473500542017493 14-15 0.042785969914316685 16-17 0.041300990481281837 18-19 0.046189047781688225 20-21 0.04801433500146023 22-23 0.060481974824648684 24-25 0.07607425887151463 26-27 0.07774486073367884 28-29 0.06790687198982294 30-31 0.0572645193864065 32-33 0.046467481425382255 34-35 0.2870032125055068 36-37 0.45684773515886806 38-39 0.43488241437856084 40-41 0.41171054780891286 42-43 0.441967003756998 44-45 0.44960846042282315 46-47 0.44954658627978 48-49 0.46560292639946943 50-51 0.45406339872192775 52-53 0.462818589962529 54-55 0.4529806012186732 56-57 0.4318815184409696 58-59 0.41155586245130504 60-61 0.3803403572860516 62-63 0.4374811283863718 64-65 0.4751624814996312 66-67 0.4753481039287606 68-69 0.4869495057493453 70-71 0.4846292253852284 72-73 0.4746674883552863 74-75 0.46826351455032345 76-77 0.44929908970760757 78-79 0.4252919222068774 80-81 0.43017997950728387 82-83 0.44113170282591585 84-85 0.43912079317701447 86-87 0.44991783113803874 88-89 0.461271736386451 90-91 0.46591229711468496 92-93 0.4676138360483707 94-95 0.4719140889898675 96-97 0.4727803269924712 98-99 0.46718071704706887 100-101 0.4544965177232295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1616184.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.40161728535125 #Duplication Level Percentage of deduplicated Percentage of total 1 81.56975834033972 49.26945325331788 2 11.449976723799224 13.831942239942016 3 3.0920471721165885 5.602939497553164 4 1.2682537898284783 3.0641832013566415 5 0.6279233101795457 1.896379173300791 6 0.4085352138707295 1.4805712581485357 7 0.25846823624243637 1.0928329640154775 8 0.17347370863719297 0.838247404653941 9 0.14196589567368273 0.7717472728248499 >10 0.8810544323577477 10.312529890523468 >50 0.0815285826152735 3.4176734712964465 >100 0.044243150510652966 4.768715148845627 >500 0.0015396910160447356 0.6169608555608832 >1k 9.238146096268413E-4 1.3630674715612519 >5k 2.052921354726314E-4 0.9591382208347767 >10k+ 1.026460677363157E-4 0.7136186762642714 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11510 0.712171386426298 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9159 0.5667052761319256 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6311 0.3904877167451231 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4328 0.2677912910906184 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3878 0.2399479267212149 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2546 0.1575315681877806 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2372 0.1467654672982779 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2344 0.1450329912930706 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2070 0.12807947609925602 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1797 0.11118783504848458 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.187414304311885E-5 0.0 22-23 0.0 0.0 0.0 4.949931443449508E-4 0.0 24-25 6.187414304311885E-5 0.0 0.0 0.001454042361513293 0.0 26-27 6.187414304311885E-5 0.0 0.0 0.0026605881508541106 0.0 28-29 6.187414304311885E-5 0.0 0.0 0.0054139875162729 0.0 30-31 6.187414304311885E-5 0.0 0.0 0.013890745113180182 0.0 32-33 6.187414304311885E-5 0.0 0.0 0.03316454067111171 0.0 34-35 6.187414304311885E-5 0.0 0.0 0.06623627012765873 0.0 36-37 6.187414304311885E-5 0.0 0.0 0.11440529048672676 0.0 38-39 6.187414304311885E-5 0.0 0.0 0.18410651262480016 0.0 40-41 6.187414304311885E-5 0.0 0.0 0.25881954034936616 0.0 42-43 6.187414304311885E-5 0.0 0.0 0.33461536557718674 0.0 44-45 1.237482860862377E-4 0.0 0.0 0.43191245551249113 0.0 46-47 1.8562242912935657E-4 0.0 0.0 0.5531548388054826 0.0 48-49 1.8562242912935657E-4 0.0 0.0 0.6859367497760156 0.0 50-51 1.8562242912935657E-4 0.0 0.0 0.8220908015423987 0.0 52-53 1.8562242912935657E-4 0.0 0.0 0.9594823361696441 0.0 54-55 1.8562242912935657E-4 0.0 0.0 1.1114142944120224 0.0 56-57 1.8562242912935657E-4 0.0 0.0 1.2969129752552928 0.0 58-59 1.8562242912935657E-4 0.0 0.0 1.5087081668918885 0.0 60-61 1.8562242912935657E-4 0.0 0.0 1.7347653484999233 0.0 62-63 1.8562242912935657E-4 0.0 0.0 1.9627715656138163 0.0 64-65 1.8562242912935657E-4 0.0 0.0 2.2001826524702635 0.0 66-67 2.474965721724754E-4 0.0 0.0 2.4682214401330542 0.0 68-69 2.474965721724754E-4 0.0 0.0 2.7719616083317247 0.0 70-71 2.474965721724754E-4 0.0 0.0 3.1086497577008556 0.0 72-73 2.474965721724754E-4 0.0 0.0 3.450628146300174 0.0 74-75 2.474965721724754E-4 0.0 0.0 3.7964736688396865 0.0 76-77 2.474965721724754E-4 0.0 0.0 4.168089771956659 0.0 78-79 2.474965721724754E-4 0.0 0.0 4.581316236270128 0.0 80-81 2.474965721724754E-4 0.0 0.0 5.040143944006376 0.0 82-83 2.474965721724754E-4 0.0 0.0 5.50088975017696 0.0 84-85 2.7843364369403484E-4 0.0 0.0 5.963460843567317 0.0 86-87 3.0937071521559427E-4 0.0 0.0 6.46049583463269 0.0 88-89 3.0937071521559427E-4 0.0 0.0 6.987013854858111 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1820 0.0 42.428596 2 GTACCTG 2225 0.0 34.93912 1 TATCACG 120 1.5419027E-7 31.582838 2 ACCTGGG 2540 0.0 30.028566 3 GTATCAA 14170 0.0 28.76913 1 GGTATCA 9710 0.0 25.971111 1 GTACATG 10505 0.0 24.63739 1 TACATGG 10470 0.0 24.343222 2 TATCAAC 17100 0.0 23.548609 2 ATCAACG 17095 0.0 23.527784 3 TCAACGC 17150 0.0 23.507576 4 ACATGGG 10550 0.0 23.215633 3 CAACGCA 17415 0.0 23.122663 5 AACGCAG 17780 0.0 22.736958 6 AGTACTT 9335 0.0 22.417894 12-13 GAGTACT 9185 0.0 21.856148 12-13 ATCACGC 175 4.1450858E-6 21.656805 3 ACGCAGA 19145 0.0 20.994179 7 CATGGGG 3340 0.0 20.992188 4 CGCAGAG 19210 0.0 20.97178 8 >>END_MODULE