FastQCFastQC Report
Thu 26 May 2016
SRR938715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938715_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616184
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110780.6854417566316707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99460.6154002267068601No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67940.4203729278349495No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40610.25127089489810567No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38450.237906080000792No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.1611202684842815No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25340.15678907847126317No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24960.15443786103562465No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20940.12956445553229087No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19750.12220143251015973No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG608.3398576E-539.544892
TACCTGG18150.039.2180752
ATAACGC1509.658834E-1031.6359163
GGTATCA97150.031.4289021
GTACCTG23950.030.032081
GTACCCG2151.8189894E-1228.8017481
TATAACG1354.2629654E-728.1208132
ACCTGGG24100.027.9603733
GTATCAA135600.026.4163071
ACCGTAT909.0000324E-426.3632628
TAACGCA2453.0195224E-1023.2427144
ACACCGT2154.4701665E-822.0715686
GTACATG106600.022.029451
GAGTACT92550.021.84262512-13
ATCAACG164800.021.653713
GCGTATC1100.00283131821.6516631
TATCAAC166000.021.5829352
TCAACGC166900.021.3812564
GTACACG3351.8189894E-1221.3285051
TACATGG107150.021.3022042
Produced by FastQC (version 0.11.5)