FastQCFastQC Report
Thu 26 May 2016
SRR938714_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938714_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612327
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117120.7264035149197402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92580.5742011390989544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62840.38974724109935516No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42380.26284990575733086No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37900.2350639789571222No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25950.16094750010388711No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24280.15058979971184505No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23790.14755071396807223No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.12745553476434993No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17990.11157786230708783No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18350.042.9195982
GTACCTG23050.034.434971
ACCTGGG25300.030.004273
TATCACG1452.4288966E-829.4481582
GTATCAA132800.028.2379461
GGTATCA93800.025.7403871
GTACATG103500.024.8434031
TACATGG105150.023.6882882
TATCAAC159450.023.4468942
TCAACGC159750.023.432564
ATCAACG159950.023.4032613
CAACGCA161600.023.1055855
AACGCAG164000.022.8528216
ACATGGG104050.022.570793
GAGTACT86550.022.23700312-13
CATGGGG30000.021.8243584
ATCACGC2005.450056E-721.3499133
AGTACTT89400.021.20956412-13
ACGCAGA178200.020.8986667
ATGGGCG2056.910268E-720.8291845
Produced by FastQC (version 0.11.5)