##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938714_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1612327 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.648063327104243 33.0 31.0 34.0 30.0 34.0 2 31.694903081074745 33.0 31.0 34.0 30.0 34.0 3 31.696709166316758 33.0 31.0 34.0 28.0 34.0 4 35.26964753427809 37.0 35.0 37.0 33.0 37.0 5 35.31565681155249 37.0 35.0 37.0 33.0 37.0 6 35.39280493349054 37.0 35.0 37.0 33.0 37.0 7 35.31355301995191 37.0 35.0 37.0 33.0 37.0 8 35.285809888440745 37.0 35.0 37.0 33.0 37.0 9 36.869278998615044 39.0 37.0 39.0 33.0 39.0 10-11 36.74823097299741 39.0 37.0 39.0 32.0 39.0 12-13 36.63449225870434 39.0 37.0 39.0 32.0 39.0 14-15 37.77985110960742 40.0 38.0 41.0 32.0 41.0 16-17 37.66463192640202 40.0 37.5 41.0 32.0 41.0 18-19 37.64670845306194 40.0 37.0 41.0 32.0 41.0 20-21 37.55220374030826 40.0 37.0 41.0 32.0 41.0 22-23 37.553124149133524 40.0 37.0 41.0 32.0 41.0 24-25 37.48576963606018 40.0 37.0 41.0 32.0 41.0 26-27 37.34403256907563 40.0 37.0 41.0 32.0 41.0 28-29 37.161006111043235 40.0 37.0 41.0 31.0 41.0 30-31 36.898674090305505 39.5 36.0 41.0 30.5 41.0 32-33 36.67385089997252 39.0 35.5 41.0 30.0 41.0 34-35 36.36099345852299 39.0 35.0 41.0 30.0 41.0 36-37 36.13897366973325 39.0 35.0 41.0 30.0 41.0 38-39 35.96458069609949 39.0 35.0 41.0 29.0 41.0 40-41 35.82057020691212 39.0 35.0 40.5 28.5 41.0 42-43 35.76402801664923 39.0 35.0 41.0 27.5 41.0 44-45 35.53614713392506 39.0 35.0 41.0 26.5 41.0 46-47 35.166022773295985 39.0 35.0 41.0 24.5 41.0 48-49 34.839004742834426 39.0 35.0 41.0 24.0 41.0 50-51 33.702571810805125 38.0 33.0 40.0 21.5 40.5 52-53 33.78533262793466 38.0 33.5 39.5 22.0 40.5 54-55 34.2763933742969 38.0 34.0 40.0 22.0 41.0 56-57 34.18495813814443 38.0 34.0 40.0 21.5 41.0 58-59 33.996876874232086 38.0 34.0 40.0 20.5 41.0 60-61 33.700384599401985 38.0 34.0 40.0 19.5 41.0 62-63 33.319518931333405 37.0 33.0 40.0 17.5 41.0 64-65 32.93226622143027 37.0 33.0 40.0 13.5 41.0 66-67 32.55883918088577 36.0 32.0 39.0 12.0 41.0 68-69 32.15357833739682 36.0 32.0 39.0 8.0 41.0 70-71 31.736051061602268 35.0 31.0 38.5 7.0 40.0 72-73 31.313704974239098 35.0 31.0 38.0 3.5 40.0 74-75 30.855613656534935 35.0 31.0 37.0 2.0 39.0 76-77 30.392477146385318 35.0 30.0 36.5 2.0 39.0 78-79 29.95161062241096 34.5 30.0 36.0 2.0 39.0 80-81 29.486858745155295 34.0 29.0 36.0 2.0 37.5 82-83 29.07513643324214 34.0 29.0 35.0 2.0 37.0 84-85 28.72741168509862 34.0 29.0 35.0 2.0 37.0 86-87 28.291386300669778 34.0 28.5 35.0 2.0 36.0 88-89 27.83649129488001 34.0 27.0 35.0 2.0 36.0 90-91 27.385286917604184 34.0 26.0 35.0 2.0 35.5 92-93 26.930941738245405 33.5 25.5 35.0 2.0 35.0 94-95 26.423449771665425 33.0 25.0 35.0 2.0 35.0 96-97 25.827431718255667 33.0 23.5 35.0 2.0 35.0 98-99 25.063359975985023 32.5 13.0 35.0 2.0 35.0 100-101 22.561733755001313 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.00852763888498842 1101 2 0.19953502464792194 1101 3 0.29469744429531985 1101 4 0.013722259228480027 1101 5 0.05016742440167121 1101 6 0.1059430669324044 1101 7 0.16478221607654575 1101 8 0.2125266889274755 1101 9 0.11733660385392142 1101 10-11 0.22374684824993807 1101 12-13 0.13118153476461458 1101 14-15 0.09961908117480078 1101 16-17 0.18369515954884008 1101 18-19 0.2390334200827482 1101 20-21 0.38214842672701366 1101 22-23 0.2603136219925517 1101 24-25 0.293685056912274 1101 26-27 0.17257965094681538 1101 28-29 0.42951869890989514 1101 30-31 0.3196678128066708 1101 32-33 0.39208620951878714 1101 34-35 0.4257497542364135 1101 36-37 0.4323393435525986 1101 38-39 0.5277320428690331 1101 40-41 0.4984445973310301 1101 42-43 0.6944520320171392 1101 44-45 0.6682306595220169 1101 46-47 0.6223262211424654 1101 48-49 0.6179047564644549 1101 50-51 0.6125767998478651 1101 52-53 0.6389156464881509 1101 54-55 0.6928330408456489 1101 56-57 0.6374717057131747 1101 58-59 0.5520592525191077 1101 60-61 0.6347937545308042 1101 62-63 0.6017353437115958 1101 64-65 0.6099022210393841 1101 66-67 0.5620857622207254 1101 68-69 0.6982752857266377 1101 70-71 0.5067819564459519 1101 72-73 0.5373015626885937 1101 74-75 0.31580364893132185 1101 76-77 0.263596927767594 1101 78-79 0.28037340659384213 1101 80-81 0.32551746748630705 1101 82-83 0.3594081840796868 1101 84-85 0.3971481273747912 1101 86-87 0.3195668258948956 1101 88-89 0.279910567967228 1101 90-91 0.15669162092576272 1101 92-93 0.18333678336388104 1101 94-95 0.14723831748768745 1101 96-97 0.08617118913326038 1101 98-99 -0.001169528629283434 1101 100-101 0.28939543012261737 1102 1 0.1338606855614337 1102 2 0.14418746596643928 1102 3 0.19317560334382122 1102 4 0.20404605354192995 1102 5 0.24367925283752356 1102 6 0.3171080968394193 1102 7 0.3078007131242373 1102 8 0.23925027746222582 1102 9 0.4717658367140132 1102 10-11 0.3762376918658106 1102 12-13 0.4324503754166571 1102 14-15 0.4108912234999451 1102 16-17 0.5903668427110702 1102 18-19 0.47348330759551516 1102 20-21 0.4371612111124037 1102 22-23 0.39201659426617397 1102 24-25 0.4473092190167378 1102 26-27 0.4384311370836258 1102 28-29 0.5110922916677154 1102 30-31 0.5300546460414637 1102 32-33 0.5200348723411778 1102 34-35 0.5454538321866096 1102 36-37 0.573220583138891 1102 38-39 0.6290749792081698 1102 40-41 0.702037278366376 1102 42-43 0.617360268539791 1102 44-45 0.6408690034642106 1102 46-47 0.8005394017286278 1102 48-49 0.775150777180535 1102 50-51 0.7882864676074064 1102 52-53 0.6828559245965238 1102 54-55 0.4609684401561438 1102 56-57 0.5575800466608385 1102 58-59 0.5287018348881745 1102 60-61 0.5825705000025323 1102 62-63 0.34006239362828694 1102 64-65 0.2961891441104001 1102 66-67 0.36161009094451657 1102 68-69 0.3060764482227043 1102 70-71 0.24022944064783047 1102 72-73 0.27382651335545205 1102 74-75 0.3030753152012764 1102 76-77 0.35672138548698484 1102 78-79 0.3528455151344225 1102 80-81 0.3719938912368548 1102 82-83 0.30261172838736883 1102 84-85 0.15476424261085953 1102 86-87 0.18710331706099836 1102 88-89 0.19096848545611422 1102 90-91 0.2027818257062819 1102 92-93 0.30253562854612426 1102 94-95 0.19765908885066352 1102 96-97 0.15205607591658676 1102 98-99 0.11107353593762426 1102 100-101 0.6351404948891606 1103 1 0.07383295474033602 1103 2 0.08837633944931866 1103 3 0.24438455318142616 1103 4 0.16097211101690334 1103 5 0.2175477023807204 1103 6 0.15242880568479222 1103 7 0.15770099613055777 1103 8 0.25417743657256153 1103 9 0.27823509538939106 1103 10-11 0.2537975695463004 1103 12-13 0.32529259464208593 1103 14-15 0.345455982564701 1103 16-17 0.4122143611824569 1103 18-19 0.46250684080602156 1103 20-21 0.29165702299867036 1103 22-23 0.3463718526756381 1103 24-25 0.34918864692610185 1103 26-27 0.3763672802202507 1103 28-29 0.4040427750577109 1103 30-31 0.5441128427783468 1103 32-33 0.6353903396814857 1103 34-35 0.6878240576419827 1103 36-37 0.6818617729050729 1103 38-39 0.5444931258016439 1103 40-41 0.6238809980951459 1103 42-43 0.7149974506325165 1103 44-45 0.7459165827154806 1103 46-47 0.7499923827918948 1103 48-49 0.8612055930364875 1103 50-51 0.7496494564580942 1103 52-53 0.7631432994754377 1103 54-55 0.6815623735641836 1103 56-57 0.7608792546229992 1103 58-59 0.6532350643892073 1103 60-61 0.7230850396898916 1103 62-63 0.7780906562733492 1103 64-65 0.5746179841643411 1103 66-67 0.45944100891066597 1103 68-69 0.5245943687909929 1103 70-71 0.6451687557345345 1103 72-73 0.6632517635976249 1103 74-75 0.4046252021886829 1103 76-77 0.370078714211175 1103 78-79 0.4918614508596981 1103 80-81 0.49257167998877804 1103 82-83 0.33820404732980336 1103 84-85 0.5015220157879376 1103 86-87 0.4843682223773378 1103 88-89 0.36605443200780385 1103 90-91 0.37293890371346095 1103 92-93 0.2947918917230119 1103 94-95 0.4018756931546399 1103 96-97 0.4536667427858525 1103 98-99 0.5213586315709691 1103 100-101 0.888710363262728 1104 1 -0.16521624446828298 1104 2 -0.1493314605457421 1104 3 -0.23536123095174943 1104 4 -0.10811127885741456 1104 5 0.05005525226507501 1104 6 0.16593326339499015 1104 7 0.08719866512158347 1104 8 0.009379492193680505 1104 9 0.21109253418733687 1104 10-11 0.043610689715372075 1104 12-13 0.19002926569899614 1104 14-15 0.21581539317007525 1104 16-17 0.33179445647540007 1104 18-19 0.17922396673752417 1104 20-21 0.20728647144374435 1104 22-23 0.14523058003786815 1104 24-25 0.27755416585830517 1104 26-27 0.16330393721317904 1104 28-29 0.2828588163525936 1104 30-31 0.2307587175671486 1104 32-33 0.25773894763295857 1104 34-35 0.21514599155269565 1104 36-37 0.24862289627465373 1104 38-39 0.06530990070390885 1104 40-41 0.22386161261486848 1104 42-43 0.2870424477532012 1104 44-45 0.32086294005069504 1104 46-47 0.27782061983450745 1104 48-49 0.43023481759826865 1104 50-51 0.38528262995624374 1104 52-53 0.45018410591142555 1104 54-55 0.33650595611496925 1104 56-57 0.33244512629492107 1104 58-59 0.4047248005376147 1104 60-61 0.1567235993106948 1104 62-63 0.16981134571224743 1104 64-65 0.4200345534284722 1104 66-67 0.3026371804683734 1104 68-69 0.2744376694921087 1104 70-71 0.2534267802780725 1104 72-73 0.1747441224874784 1104 74-75 0.18470015937908002 1104 76-77 0.20740982771696537 1104 78-79 0.006518964538816618 1104 80-81 0.09874785599373581 1104 82-83 0.14877426553048423 1104 84-85 0.09546462336309958 1104 86-87 0.16961131059838053 1104 88-89 0.3628843358216116 1104 90-91 0.376581853575388 1104 92-93 0.4325333312279618 1104 94-95 0.34446939439397894 1104 96-97 0.06795954336057619 1104 98-99 -0.008197573968338645 1104 100-101 0.3750019779021869 1105 1 0.08592335571966458 1105 2 0.2212225027985113 1105 3 0.26819136732183324 1105 4 0.28089192263785634 1105 5 0.21703108257172232 1105 6 0.2804281018838921 1105 7 0.15463122625099146 1105 8 0.17941244243132815 1105 9 0.17145352449080775 1105 10-11 0.2065794549091109 1105 12-13 0.24333048758199283 1105 14-15 0.16614192638790826 1105 16-17 0.4142157013990655 1105 18-19 0.2497004721566114 1105 20-21 0.2915643683590119 1105 22-23 0.25105287737979864 1105 24-25 0.2784721925851912 1105 26-27 0.2073990972619555 1105 28-29 0.3073534470003878 1105 30-31 0.2430374874150445 1105 32-33 0.2804468870888499 1105 34-35 0.2106384376420536 1105 36-37 0.3582696554751479 1105 38-39 0.2844313934780942 1105 40-41 0.3209948528650841 1105 42-43 0.14432854028851239 1105 44-45 0.20160482886610254 1105 46-47 0.3883347444931502 1105 48-49 0.47214857393278464 1105 50-51 0.471683104130868 1105 52-53 0.4162521902028615 1105 54-55 0.4476584498741758 1105 56-57 0.32594452466916124 1105 58-59 0.32102718855765744 1105 60-61 0.3113555238524981 1105 62-63 0.27605693372813533 1105 64-65 0.23430273377219635 1105 66-67 0.17001692768905485 1105 68-69 0.16366392902774152 1105 70-71 0.24539075250753584 1105 72-73 0.27335804502064676 1105 74-75 0.3269538472596416 1105 76-77 0.3391788598380643 1105 78-79 0.24279885375950983 1105 80-81 0.386399570719945 1105 82-83 0.374515868564373 1105 84-85 0.38745947484207477 1105 86-87 0.22843795107706555 1105 88-89 0.2948245399364211 1105 90-91 0.39118213430933224 1105 92-93 0.24063104326856788 1105 94-95 0.39282492589935103 1105 96-97 0.5491770171558947 1105 98-99 0.5222121773423609 1105 100-101 1.2000591527626163 1106 1 -0.08603973550891197 1106 2 -0.14460825821236156 1106 3 -0.06319550925499584 1106 4 -0.01736012386368202 1106 5 0.04179156996824673 1106 6 0.006075761124535006 1106 7 -0.041659870520966535 1106 8 -0.1229459464403746 1106 9 -0.03155553397787969 1106 10-11 -0.006933944601591691 1106 12-13 -0.029691888558964763 1106 14-15 -0.0022925585238979806 1106 16-17 0.1735973778106299 1106 18-19 0.1898684680668552 1106 20-21 0.1505778439397929 1106 22-23 0.18192572209083835 1106 24-25 0.19057465429958853 1106 26-27 0.0917190343292873 1106 28-29 0.038963234155581006 1106 30-31 -0.0310698788368029 1106 32-33 0.10122919758601512 1106 34-35 0.09456486525397878 1106 36-37 0.19646011765678395 1106 38-39 0.13417080838018336 1106 40-41 0.29390579174295084 1106 42-43 0.0917927747314593 1106 44-45 0.016315773368113184 1106 46-47 0.004128011245335017 1106 48-49 0.14921196590857733 1106 50-51 0.04689280158826392 1106 52-53 -0.1596888255598614 1106 54-55 -0.13073032809419516 1106 56-57 -0.10729478460007158 1106 58-59 0.07017689808530747 1106 60-61 0.09695109491529763 1106 62-63 0.05200692648164562 1106 64-65 0.04843954743798662 1106 66-67 0.08948304549745245 1106 68-69 0.1327668572572449 1106 70-71 0.04021279538221734 1106 72-73 0.15250575725798043 1106 74-75 0.09744445963462312 1106 76-77 0.216435635663494 1106 78-79 0.19261873710910038 1106 80-81 0.14989263680573828 1106 82-83 0.1858900043036833 1106 84-85 0.21478615582866212 1106 86-87 0.29517755128732404 1106 88-89 0.529732199508743 1106 90-91 0.40752151936022685 1106 92-93 0.4712809413490753 1106 94-95 0.6307315853844244 1106 96-97 0.5758383192593932 1106 98-99 0.8205273151445773 1106 100-101 1.202812096602706 1107 1 0.014294128798962191 1107 2 -0.0019778981550047092 1107 3 0.1701229800189914 1107 4 0.18673371527099647 1107 5 0.12096194311305197 1107 6 -0.05085937251374162 1107 7 0.07937074997885674 1107 8 0.016470983263744188 1107 9 0.02093110001733578 1107 10-11 0.20066984313779557 1107 12-13 0.12402179385292555 1107 14-15 0.2353713567292317 1107 16-17 0.3872551100956727 1107 18-19 0.16278949222709826 1107 20-21 0.17578407292809572 1107 22-23 0.1626723601291573 1107 24-25 0.015113699075293141 1107 26-27 0.09446808879174284 1107 28-29 0.19934567374225765 1107 30-31 0.125118478847682 1107 32-33 0.01707735410990807 1107 34-35 0.20091892111192777 1107 36-37 0.28624871139447094 1107 38-39 0.24584495333152034 1107 40-41 0.18762831526207435 1107 42-43 0.38859611199538335 1107 44-45 0.6355545816187913 1107 46-47 0.7741338291003785 1107 48-49 0.7881059056489548 1107 50-51 0.8527492003759036 1107 52-53 0.559782950947401 1107 54-55 0.5028077734123073 1107 56-57 0.5887317256559399 1107 58-59 0.6850996781271945 1107 60-61 0.652966005754763 1107 62-63 0.48668834719967435 1107 64-65 0.5021086613783012 1107 66-67 0.33901077576093286 1107 68-69 0.5531941701812642 1107 70-71 0.5217388372700462 1107 72-73 0.46023417702620506 1107 74-75 0.6578109663556404 1107 76-77 0.468085267955459 1107 78-79 0.42932595455206624 1107 80-81 0.4538753814033534 1107 82-83 0.47809313491217154 1107 84-85 0.6248117391475709 1107 86-87 0.560461942051198 1107 88-89 0.5250147647073717 1107 90-91 0.601404351461575 1107 92-93 0.766649231897766 1107 94-95 0.7603481695014267 1107 96-97 0.9621061489163729 1107 98-99 1.1009118162045297 1107 100-101 1.3411376794497976 1108 1 0.3374308575589602 1108 2 0.2548773568149407 1108 3 0.26319877629980937 1108 4 0.237674744330576 1108 5 0.27286504494416164 1108 6 0.3066863328638618 1108 7 0.4055177927656004 1108 8 0.370479980905003 1108 9 0.39466662168273103 1108 10-11 0.37493203936309527 1108 12-13 0.5322192524786615 1108 14-15 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-1.5228432788976818 2316 84-85 -1.4244193725406475 2316 86-87 -1.6832830201965017 2316 88-89 -1.7156159347557711 2316 90-91 -2.156969035976445 2316 92-93 -1.88925395505915 2316 94-95 -1.8332694117684767 2316 96-97 -1.882006938932225 2316 98-99 -2.3938732461139764 2316 100-101 -2.140043395436358 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7925.0 3 1749.0 4 1651.0 5 1866.0 6 2188.0 7 2574.0 8 3172.0 9 3925.0 10 4781.0 11 6200.0 12 8017.0 13 10253.0 14 10978.0 15 12355.0 16 15142.0 17 18947.0 18 16806.0 19 11336.0 20 10976.0 21 11658.0 22 12502.0 23 13641.0 24 15458.0 25 17582.0 26 20363.0 27 23389.0 28 27587.0 29 33028.0 30 39406.0 31 48464.0 32 59335.0 33 76024.0 34 101114.0 35 146977.0 36 203492.0 37 295813.0 38 271854.0 39 43781.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.072303969582144 19.17409358067118 14.233742101227422 21.519860348519252 2 15.216783581700128 21.058435152536223 41.2549170164178 22.469864249345846 3 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20.318723737961875 22-23 21.533781177310964 26.622709115171915 30.921563684414537 20.921946023102585 24-25 21.20420304589354 26.717724758118482 31.40430057863834 20.67377161734964 26-27 21.128217254941006 26.810530442574798 31.273398217752774 20.787854084731418 28-29 20.751371619011238 26.893531009881134 31.555542560160553 20.799554810947072 30-31 20.954601954271382 26.96867760928442 31.374044780746996 20.7026756556972 32-33 20.679649053039718 27.105650693443355 31.131350350449864 21.08334990306706 34-35 21.254030759808725 27.01148996052174 30.97036360705468 20.764115672614857 36-37 21.39441127209559 26.774993929356388 30.875604729498345 20.954990069049682 38-39 21.153878477182957 26.921434418703093 31.08898039984686 20.83570670426709 40-41 21.157682829690845 26.900638472644 31.161603275750487 20.78007542191467 42-43 21.0218789537817 27.13223702537112 30.685843496773167 21.16004052407401 44-45 21.038434218201672 27.43529707657895 30.322734876296686 21.203533828922687 46-47 21.18694269647357 27.42880445017907 29.81707136753667 21.567181485810696 48-49 21.360379223650106 27.518311240494114 29.659492656044463 21.46181687981132 50-51 21.47013369663218 27.797841128152506 29.400961629465066 21.33106354575025 52-53 21.408126313010495 27.85176228268003 29.508163547404116 21.23194785690536 54-55 21.093507764607438 27.712631698226925 29.640988858961954 21.552871678203687 56-57 21.299647079292466 28.209099372125458 29.15238959906353 21.33886394951855 58-59 21.313739285315325 27.701242525353198 29.32295732920683 21.662060860124647 60-61 21.335418304298962 27.582554553957955 29.714009132272373 21.368018009470713 62-63 21.455440548853087 27.562523675841504 29.614933156698974 21.367102618606435 64-65 21.239953182054116 27.505885715762506 29.638474910706197 21.61568619147718 66-67 21.06359578104059 27.966331278990396 29.2921360350351 21.67793690493391 68-69 21.07894350866059 27.907392431221155 29.383350081634347 21.63031397848391 70-71 21.004915149522184 27.914555369545504 29.21026277241338 21.87026670851893 72-73 21.163866464646087 27.800809203270443 29.070163540765627 21.965160791317846 74-75 21.109119424301838 27.9584255357705 29.037018908777736 21.89543613114993 76-77 21.2613870768702 27.763142574825807 29.16760320204819 21.8078671462558 78-79 21.125749695764355 27.865713031867713 29.102108018437768 21.906429253930167 80-81 21.054595846061616 28.219277346947795 28.91966226138947 21.806464545601127 82-83 21.269169659266936 28.103971898611864 28.70707839201486 21.91978005010634 84-85 21.16055405007868 27.84047168034567 28.751114839782154 22.247859429793497 86-87 21.325498064892486 27.719928790284648 28.953896409724187 22.000676735098676 88-89 21.429623221197417 27.533751758528336 28.861967939137095 22.17465708113715 90-91 21.22955161057193 27.78739061783351 29.071843879035768 21.91121389255879 92-93 21.31156422763215 27.835725973312243 28.880661729333024 21.972048069722582 94-95 21.13403153360753 27.728112941035793 28.761244653262352 22.376610872094325 96-97 21.43152752342288 27.539210963343937 28.544603512054216 22.484658001178968 98-99 21.428191084385297 27.45492042821506 28.49578233885339 22.621106148546247 100-101 21.683507490425665 27.071626786840557 28.96074818957772 22.284117533156053 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4503.0 1 2933.5 2 2754.5 3 3478.0 4 2187.0 5 1686.0 6 2353.5 7 4233.0 8 6164.5 9 6476.5 10 5084.5 11 3683.0 12 3378.5 13 3440.5 14 3576.5 15 3666.5 16 3709.0 17 3840.0 18 4033.5 19 4222.5 20 4571.5 21 5113.0 22 5874.5 23 6754.0 24 7676.0 25 9118.5 26 11201.5 27 13675.0 28 15934.5 29 18323.5 30 21254.0 31 24172.0 32 27760.0 33 31066.5 34 34340.0 35 37540.5 36 40985.0 37 44418.0 38 46164.5 39 46853.5 40 47169.0 41 49295.5 42 51293.0 43 52558.5 44 54321.5 45 55432.0 46 55630.0 47 56895.0 48 64521.5 49 65352.0 50 58322.5 51 54525.0 52 50343.0 53 48103.5 54 46428.5 55 42340.5 56 37923.5 57 33646.5 58 29920.5 59 26421.5 60 22498.5 61 18677.5 62 15256.0 63 11996.0 64 8970.5 65 6904.5 66 5117.5 67 3980.0 68 3061.5 69 2053.5 70 1561.0 71 1236.0 72 1021.5 73 920.5 74 716.0 75 544.0 76 405.0 77 270.5 78 195.5 79 147.0 80 106.5 81 69.5 82 52.0 83 51.5 84 35.5 85 19.5 86 18.5 87 13.5 88 6.0 89 4.5 90 7.5 91 6.5 92 4.0 93 2.5 94 1.0 95 2.0 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2681838113484423 2 0.161939854632466 3 0.1876790502174807 4 0.15896279104672936 5 0.22811749725707006 6 0.18991184790678317 7 0.23159073810709616 8 0.23841317549107596 9 0.14153456463856276 10-11 0.19843989463675793 12-13 0.20330863404259808 14-15 0.16674657188026992 16-17 0.27810735663423114 18-19 0.22417909022177263 20-21 0.1900979143808917 22-23 0.23602532240668303 24-25 0.2255435776985686 26-27 0.33287292218017805 28-29 0.30514901753800566 30-31 0.2800610546123708 32-33 0.18401974289334608 34-35 0.3485645281633316 36-37 0.3868321996716547 38-39 0.3697761062117052 40-41 0.33287292218017805 42-43 0.36434916738353945 44-45 0.3736214800099483 46-47 0.37830415294167996 48-49 0.39784113272307664 50-51 0.3909256621020426 52-53 0.38980926325739135 54-55 0.3841032247180628 56-57 0.3644111895415756 58-59 0.33978839280121215 60-61 0.30704069335810913 62-63 0.3718538485059172 64-65 0.40515974737134586 66-67 0.4107727526736202 68-69 0.41502127049909854 70-71 0.4142459935236462 72-73 0.406245135136979 74-75 0.4022447059436454 76-77 0.38639804456540144 78-79 0.3623334472473636 80-81 0.37188485958493533 82-83 0.3810021168162538 84-85 0.38171537163366986 86-87 0.3774358427291734 88-89 0.3861189448542386 90-91 0.4060280575838524 92-93 0.40528379168741824 94-95 0.41399790489150157 96-97 0.41464913755088145 98-99 0.411734096123181 100-101 0.3851576014046778 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1612327.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.33024944201251 #Duplication Level Percentage of deduplicated Percentage of total 1 81.37265374053612 49.09232497925056 2 11.553821226359789 13.940898331894097 3 3.1849901212322647 5.764537454528646 4 1.2777795126636882 3.0835502692357393 5 0.6326112058738182 1.908279592508989 6 0.3856813950055637 1.3960952859497409 7 0.2660509329130194 1.1235643402845816 8 0.17606531596124209 0.8497651544022784 9 0.14998644511904236 0.814384768225729 >10 0.8722460855855887 10.213166591683374 >50 0.08283624006298759 3.4947273146309694 >100 0.04249319243328898 4.653557508714689 >500 0.0015469923631135076 0.6019190864372436 >1k 9.281954178681045E-4 1.3674775358401634 >5k 2.0626564841513435E-4 0.9670277487500144 >10k+ 1.0313282420756717E-4 0.7287240376631173 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11712 0.7264035149197402 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9258 0.5742011390989544 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6284 0.38974724109935516 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4238 0.26284990575733086 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3790 0.2350639789571222 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2595 0.16094750010388711 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2428 0.15058979971184505 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2379 0.14755071396807223 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2055 0.12745553476434993 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1799 0.11157786230708783 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.101107901809E-5 0.0 12-13 0.0 0.0 0.0 1.2404431607236E-4 0.0 14-15 0.0 0.0 0.0 1.5505539509045002E-4 0.0 16-17 3.101107901809E-5 0.0 0.0 1.8606647410854003E-4 0.0 18-19 6.202215803618E-5 0.0 0.0 1.8606647410854003E-4 0.0 20-21 6.202215803618E-5 0.0 0.0 2.7909971116281006E-4 0.0 22-23 6.202215803618E-5 0.0 0.0 7.1325481741607E-4 0.0 24-25 6.202215803618E-5 0.0 0.0 0.0016125761089406802 0.0 26-27 6.202215803618E-5 0.0 0.0 0.00275998603261001 0.0 28-29 6.202215803618E-5 0.0 0.0 0.0058300828554009206 0.0 30-31 6.202215803618E-5 0.0 0.0 0.014234085269303311 0.0 32-33 6.202215803618E-5 0.0 0.0 0.030700968227909103 0.0 34-35 6.202215803618E-5 0.0 0.0 0.05966531603080516 0.0 36-37 6.202215803618E-5 0.0 0.0 0.10934506461778534 0.0 38-39 6.202215803618E-5 0.0 0.0 0.177104272272312 0.0 40-41 6.202215803618E-5 0.0 0.0 0.2469412222210507 0.0 42-43 6.202215803618E-5 0.0 0.0 0.31777052669836825 0.0 44-45 1.2404431607236E-4 0.0 0.0 0.4132226279160493 0.0 46-47 1.5505539509045002E-4 0.0 0.0 0.529545185312905 0.0 48-49 1.8606647410854003E-4 0.0 0.0 0.6531553462790116 0.0 50-51 1.8606647410854003E-4 0.0 0.0 0.783867044340261 0.0 52-53 1.8606647410854003E-4 0.0 0.0 0.9168425511698309 0.0 54-55 1.8606647410854003E-4 0.0 0.0 1.0660988744838982 0.0 56-57 2.4808863214472E-4 0.0 0.0 1.2436062907834453 6.202215803618E-5 58-59 2.4808863214472E-4 0.0 0.0 1.4493958111474905 6.202215803618E-5 60-61 2.4808863214472E-4 0.0 0.0 1.6784436407751033 6.202215803618E-5 62-63 2.4808863214472E-4 0.0 0.0 1.9013512767571341 6.202215803618E-5 64-65 2.4808863214472E-4 0.0 0.0 2.124910145398545 6.202215803618E-5 66-67 2.4808863214472E-4 0.0 0.0 2.387977128709003 6.202215803618E-5 68-69 2.4808863214472E-4 0.0 0.0 2.6850942767813226 6.202215803618E-5 70-71 2.7909971116281006E-4 0.0 0.0 3.013315537108787 6.202215803618E-5 72-73 3.1011079018090004E-4 0.0 0.0 3.344513861021989 6.202215803618E-5 74-75 3.1011079018090004E-4 0.0 0.0 3.687713472515191 6.202215803618E-5 76-77 3.1011079018090004E-4 0.0 0.0 4.059040132677801 6.202215803618E-5 78-79 3.1011079018090004E-4 0.0 0.0 4.470060973983566 6.202215803618E-5 80-81 3.1011079018090004E-4 0.0 0.0 4.919845664061943 6.202215803618E-5 82-83 3.1011079018090004E-4 0.0 0.0 5.375987625339029 6.202215803618E-5 84-85 3.1011079018090004E-4 0.0 0.0 5.844347951749242 6.202215803618E-5 86-87 3.1011079018090004E-4 0.0 0.0 6.335315354763643 6.202215803618E-5 88-89 3.1011079018090004E-4 0.0 0.0 6.864457396049312 6.202215803618E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1835 0.0 42.919598 2 GTACCTG 2305 0.0 34.43497 1 ACCTGGG 2530 0.0 30.00427 3 TATCACG 145 2.4288966E-8 29.448158 2 GTATCAA 13280 0.0 28.237946 1 GGTATCA 9380 0.0 25.740387 1 GTACATG 10350 0.0 24.843403 1 TACATGG 10515 0.0 23.688288 2 TATCAAC 15945 0.0 23.446894 2 TCAACGC 15975 0.0 23.43256 4 ATCAACG 15995 0.0 23.403261 3 CAACGCA 16160 0.0 23.105585 5 AACGCAG 16400 0.0 22.852821 6 ACATGGG 10405 0.0 22.57079 3 GAGTACT 8655 0.0 22.237003 12-13 CATGGGG 3000 0.0 21.824358 4 ATCACGC 200 5.450056E-7 21.349913 3 AGTACTT 8940 0.0 21.209564 12-13 ACGCAGA 17820 0.0 20.898666 7 ATGGGCG 205 6.910268E-7 20.829184 5 >>END_MODULE