FastQCFastQC Report
Thu 26 May 2016
SRR938714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938714_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612327
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108320.6718240158479019No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99160.6150117190867609No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67810.4205722536433366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41740.25888048764301536No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37960.2354361119053393No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26560.16473085174409408No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25610.15883874673065698No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25300.15691605983153542No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20860.1293782216634715No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20040.12429240470450474No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG16200.042.4771582
GTACCTG20350.033.425061
GTACCCG1601.879016E-929.7290171
ACCTGGG22550.028.621663
GGTATCA99700.028.2917671
GTATCAA138600.025.9453241
CGTATCA950.001233226624.9774932
GCGTATC1151.2699577E-424.8172661
TATCACG1151.2899289E-424.7602982
ATGGGCG2301.364242E-1024.7602985
GGATCGC1000.001665329823.7219968
GTACATG102350.023.655411
TACATGG104250.023.0799242
GTACACG3251.8189894E-1221.9537351
ACATGGG103100.021.8183613
GAGTACT92150.021.54873312-13
ATCAACG166550.021.3707163
TATCAAC167850.021.3182952
TCAACGC168450.021.1860144
CAACGCA170800.020.9223065
Produced by FastQC (version 0.11.5)