##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938714_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1612327 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.977031334214463 31.0 31.0 34.0 30.0 34.0 2 32.167797847458985 33.0 31.0 34.0 30.0 34.0 3 32.234876051818276 34.0 31.0 34.0 30.0 34.0 4 35.88970661658584 37.0 35.0 37.0 35.0 37.0 5 35.79871638941729 37.0 35.0 37.0 35.0 37.0 6 35.8399939962551 37.0 35.0 37.0 35.0 37.0 7 35.76728169905981 37.0 35.0 37.0 33.0 37.0 8 35.7526246226727 37.0 35.0 37.0 33.0 37.0 9 37.38093885421506 39.0 37.0 39.0 34.0 39.0 10-11 37.297913822692294 39.0 37.0 39.0 33.5 39.0 12-13 37.21214058934695 39.0 37.0 39.0 33.0 39.0 14-15 38.374077342871516 40.0 38.0 41.0 33.0 41.0 16-17 38.289853112923126 40.0 38.0 41.0 33.0 41.0 18-19 38.46790229277312 40.0 38.0 41.0 33.5 41.0 20-21 38.477699312856515 40.0 38.0 41.0 34.0 41.0 22-23 38.38329166478016 40.0 38.0 41.0 34.0 41.0 24-25 38.29604230407355 40.0 38.0 41.0 33.0 41.0 26-27 38.09666308385334 40.0 37.0 41.0 33.0 41.0 28-29 37.90532038476066 40.0 37.0 41.0 33.0 41.0 30-31 37.63216673788878 40.0 37.0 41.0 32.5 41.0 32-33 37.422349188471074 40.0 36.0 41.0 32.0 41.0 34-35 37.33937253423158 40.0 36.0 41.0 32.0 41.0 36-37 37.19130269480075 40.0 36.0 41.0 31.0 41.0 38-39 37.053538767259994 40.0 35.5 41.0 31.0 41.0 40-41 36.84919963506162 39.5 35.0 41.0 31.0 41.0 42-43 36.61931326585736 39.0 35.0 41.0 30.0 41.0 44-45 36.31497239703857 39.0 35.0 41.0 30.0 41.0 46-47 35.92515941245169 39.0 35.0 41.0 28.5 41.0 48-49 35.556620338182015 39.0 35.0 41.0 27.0 41.0 50-51 35.304159143895745 39.0 35.0 40.0 26.5 41.0 52-53 35.06596645717649 38.5 34.5 40.0 26.0 41.0 54-55 34.775875179166505 38.0 34.0 40.0 25.5 41.0 56-57 34.51499757803473 38.0 34.0 40.0 24.5 41.0 58-59 34.20611482658295 38.0 34.0 40.0 23.5 41.0 60-61 33.84791360561474 37.0 33.0 40.0 23.0 41.0 62-63 33.47918691431701 37.0 33.0 40.0 22.0 41.0 64-65 33.074631882986516 36.0 32.5 39.0 21.5 40.5 66-67 32.668210294809924 36.0 32.0 39.0 20.0 40.0 68-69 32.329791971479736 35.0 31.5 39.0 19.5 40.0 70-71 31.891949027709643 35.0 31.0 38.0 18.0 40.0 72-73 31.395794711618674 35.0 31.0 37.0 14.5 39.5 74-75 30.886732964218798 35.0 30.0 37.0 9.5 39.0 76-77 28.962460158516233 32.5 28.0 35.0 7.5 37.0 78-79 29.916061382089367 34.0 29.5 36.0 7.0 38.0 80-81 29.96654958950635 34.0 30.0 35.5 4.0 37.0 82-83 29.620214137702835 34.0 29.5 35.0 2.0 37.0 84-85 29.196296408855027 34.0 29.0 35.0 2.0 36.5 86-87 28.56340990382224 34.0 29.0 35.0 2.0 36.0 88-89 27.91804019904151 33.5 27.0 35.0 2.0 35.5 90-91 27.355571791578257 33.0 26.0 35.0 2.0 35.0 92-93 27.01686444499162 33.0 26.0 35.0 2.0 35.0 94-95 26.626741969836146 33.0 25.0 35.0 2.0 35.0 96-97 25.9415630328091 32.5 24.5 34.5 2.0 35.0 98-99 25.012295272609094 32.0 18.5 34.0 2.0 35.0 100-101 21.28655570489113 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11039085640058843 1101 2 0.19626708049180763 1101 3 0.07941418658308663 1101 4 0.08978169654569967 1101 5 -0.11370955375636527 1101 6 0.028032345923612922 1101 7 0.09288907416530634 1101 8 0.1333991204328271 1101 9 0.18001237572034512 1101 10-11 0.2534049292429401 1101 12-13 0.19210804937817727 1101 14-15 0.4857448305038332 1101 16-17 0.42006186793828704 1101 18-19 0.35659377990475605 1101 20-21 0.38978349689826075 1101 22-23 0.3054014520232613 1101 24-25 0.24003467544390844 1101 26-27 0.1849703255250219 1101 28-29 0.36912226485481625 1101 30-31 0.4259245764156532 1101 32-33 0.3065736860019186 1101 34-35 0.3544501913304501 1101 36-37 0.37651657976918784 1101 38-39 0.4787497887886403 1101 40-41 0.3859617888312954 1101 42-43 0.36533283604872224 1101 44-45 0.38630990829923206 1101 46-47 0.3881708640417614 1101 48-49 0.16960856635732569 1101 50-51 0.24453724485052675 1101 52-53 0.1760518601437724 1101 54-55 0.25304953179034584 1101 56-57 0.23719641126999846 1101 58-59 0.24370465484263804 1101 60-61 0.36399184722247213 1101 62-63 0.3987919918731606 1101 64-65 0.3552275983140518 1101 66-67 0.3529202419879951 1101 68-69 0.2887507965793503 1101 70-71 0.5167851681196289 1101 72-73 0.3762851397786804 1101 74-75 0.353851791974364 1101 76-77 0.3203479388959778 1101 78-79 0.09346394923378298 1101 80-81 0.36549169215488675 1101 82-83 0.562666392373508 1101 84-85 0.5234612656507842 1101 86-87 0.453126165810648 1101 88-89 0.4608939615772947 1101 90-91 0.3137329419142638 1101 92-93 0.32346740196557633 1101 94-95 0.4012794935848163 1101 96-97 0.5320565214834332 1101 98-99 0.562629414473264 1101 100-101 1.1359500789894987 1102 1 0.3353576477118132 1102 2 0.22912870967552834 1102 3 0.22928327140537164 1102 4 0.1843585065152169 1102 5 0.1345923880881159 1102 6 0.05361800898403857 1102 7 0.13392272819603335 1102 8 0.24635857128013328 1102 9 0.33528622263020935 1102 10-11 0.29736632270255114 1102 12-13 0.3129872926039923 1102 14-15 0.3764410228301287 1102 16-17 0.39241189135712773 1102 18-19 0.485382833706673 1102 20-21 0.43849162131758845 1102 22-23 0.40950051757299377 1102 24-25 0.45878165210952915 1102 26-27 0.4338646489507809 1102 28-29 0.5323727236338058 1102 30-31 0.5024807778023614 1102 32-33 0.5235615619511478 1102 34-35 0.6101137124496461 1102 36-37 0.5552568763054992 1102 38-39 0.4114110140932823 1102 40-41 0.4370839737710597 1102 42-43 0.5394857265483495 1102 44-45 0.5401874546186392 1102 46-47 0.41454240208394566 1102 48-49 0.4430132055919529 1102 50-51 0.47990929733944654 1102 52-53 0.3518482928011508 1102 54-55 0.35028687601664643 1102 56-57 0.3907226749277939 1102 58-59 0.40737934022390476 1102 60-61 0.2804207422369416 1102 62-63 0.27242506081669404 1102 64-65 0.37273683148128356 1102 66-67 0.44664821483748796 1102 68-69 0.5092434369196539 1102 70-71 0.45103158042948976 1102 72-73 0.4943260958817888 1102 74-75 0.4326358548750804 1102 76-77 0.44072617242408185 1102 78-79 0.20992240573999865 1102 80-81 0.5252243334165918 1102 82-83 0.5853047363157025 1102 84-85 0.591141341205347 1102 86-87 0.8426391306363108 1102 88-89 0.6406988557317952 1102 90-91 0.6699723362492094 1102 92-93 0.8350524354046165 1102 94-95 0.9872830870336813 1102 96-97 1.0794359144362815 1102 98-99 0.9448038349528503 1102 100-101 1.489361935835099 1103 1 0.1978488631195141 1103 2 0.1810309300405848 1103 3 0.10313462660680273 1103 4 -0.003664058015921512 1103 5 -0.044254313653603106 1103 6 0.05525716642292622 1103 7 0.04710816381684424 1103 8 0.019256442084895298 1103 9 0.11930491244388008 1103 10-11 0.12415125688934125 1103 12-13 0.16538714825176726 1103 14-15 0.2223003933784753 1103 16-17 0.08258739326599596 1103 18-19 -0.1492161140416286 1103 20-21 0.08161209843966333 1103 22-23 0.18427306785884667 1103 24-25 0.1530180098751046 1103 26-27 0.25109574545783886 1103 28-29 0.08708221479223965 1103 30-31 0.2293663786684732 1103 32-33 0.2875960165466793 1103 34-35 0.34665096861785827 1103 36-37 0.455210341708451 1103 38-39 0.3877676400500931 1103 40-41 0.4391466910116506 1103 42-43 0.44531109835028815 1103 44-45 0.5317713403524635 1103 46-47 0.6348476310531552 1103 48-49 0.6535490182379604 1103 50-51 0.6135709053472027 1103 52-53 0.7661315650380374 1103 54-55 0.636436660438001 1103 56-57 0.4717593517877603 1103 58-59 0.4810967897481149 1103 60-61 0.5502872164339863 1103 62-63 0.5485186238473361 1103 64-65 0.49301167050955996 1103 66-67 0.3904144782043204 1103 68-69 0.33242051530670125 1103 70-71 0.5218638849366961 1103 72-73 0.5241937097016134 1103 74-75 0.5500806306542927 1103 76-77 0.4731474909676443 1103 78-79 0.2141114691600059 1103 80-81 0.46628874109764595 1103 82-83 0.5329219750096961 1103 84-85 0.45263918541891357 1103 86-87 0.5765666506271963 1103 88-89 0.25800877614814155 1103 90-91 0.2656465180950178 1103 92-93 0.4355614669151855 1103 94-95 0.6834710488183333 1103 96-97 0.7882945822172438 1103 98-99 0.7521525025438791 1103 100-101 1.503791459662711 1104 1 0.007136788816108464 1104 2 0.007779091745902633 1104 3 0.06935979846288376 1104 4 0.02235087640604405 1104 5 -0.1337046212555606 1104 6 0.04251292362548753 1104 7 -0.09019045050142438 1104 8 -0.020053130731341184 1104 9 5.692258652842952E-4 1104 10-11 0.08196864186407282 1104 12-13 -0.016330253257635263 1104 14-15 0.008283170709631804 1104 16-17 0.05490967877611297 1104 18-19 0.06025996484587637 1104 20-21 0.10328145436658076 1104 22-23 0.03523236405187902 1104 24-25 0.04243999947156141 1104 26-27 0.08859467640561292 1104 28-29 0.10662505955445312 1104 30-31 -0.17575043785556232 1104 32-33 -0.1099798481502674 1104 34-35 0.006655872004039054 1104 36-37 0.17941995576634184 1104 38-39 0.14160606017146193 1104 40-41 0.1263547447284381 1104 42-43 -0.007970464347486939 1104 44-45 0.262344523391306 1104 46-47 0.24320182278315627 1104 48-49 -0.014915366643641903 1104 50-51 0.04855523541731088 1104 52-53 0.12377657128131148 1104 54-55 0.037932953440027006 1104 56-57 0.06135391016940872 1104 58-59 0.26537212438521607 1104 60-61 0.2222187514789411 1104 62-63 0.1477019629773082 1104 64-65 0.02867069131474409 1104 66-67 -0.05876686536150544 1104 68-69 -0.10438738028538097 1104 70-71 -0.029390184588756085 1104 72-73 -0.009695340732946534 1104 74-75 0.17964519855764038 1104 76-77 0.2028240005168911 1104 78-79 0.015744600846336 1104 80-81 0.2723419523404651 1104 82-83 0.1944269724724812 1104 84-85 0.1782221277167011 1104 86-87 0.4463752855282799 1104 88-89 0.46797348317081244 1104 90-91 0.49231789809414295 1104 92-93 0.45317488622882607 1104 94-95 0.36124844648865206 1104 96-97 0.33516772325421584 1104 98-99 0.6105647556878431 1104 100-101 1.5070539219608037 1105 1 0.12382360864681985 1105 2 0.05009888051996114 1105 3 0.03966301636275915 1105 4 0.03009629777789513 1105 5 -0.09192521934802755 1105 6 -0.14640350420948778 1105 7 -0.004572535192217231 1105 8 0.10345161955128646 1105 9 0.12675883781852093 1105 10-11 0.09856809420276846 1105 12-13 0.12132996068508817 1105 14-15 0.21712989012339534 1105 16-17 0.11912682670627817 1105 18-19 0.2293995694875619 1105 20-21 0.08170606334694952 1105 22-23 0.023096484961953934 1105 24-25 -0.02592617646741502 1105 26-27 0.024659598543138372 1105 28-29 0.06443480032464066 1105 30-31 0.08327135057258772 1105 32-33 0.2596165615431332 1105 34-35 0.34547509700910695 1105 36-37 0.3616173632455073 1105 38-39 0.5316011462139656 1105 40-41 0.35774294514367 1105 42-43 0.49223496431365277 1105 44-45 0.42141311318200536 1105 46-47 0.5797366513847635 1105 48-49 0.5425751524617866 1105 50-51 0.25482973561513234 1105 52-53 0.46472134270434395 1105 54-55 0.4819609733898105 1105 56-57 0.5984017722638697 1105 58-59 0.8169404710760517 1105 60-61 0.8644382940355229 1105 62-63 0.742602952672101 1105 64-65 0.8434803719594655 1105 66-67 0.8315986232172534 1105 68-69 0.7671094764890896 1105 70-71 0.7237458036177493 1105 72-73 0.4717880583301337 1105 74-75 0.652359373246604 1105 76-77 0.6420703761020974 1105 78-79 0.3617213538190178 1105 80-81 0.4210463228677064 1105 82-83 0.4820744190331929 1105 84-85 0.5083055313819784 1105 86-87 0.4828031501910637 1105 88-89 0.42478057619381104 1105 90-91 0.4224378224102878 1105 92-93 0.4855268384134952 1105 94-95 0.8276399834958745 1105 96-97 0.9762975846019302 1105 98-99 1.2727427968299878 1105 100-101 1.8075687740633022 1106 1 -0.01492641929361227 1106 2 0.01994015722830511 1106 3 0.07676142220782367 1106 4 -0.0328208709152662 1106 5 -0.045119045197289154 1106 6 -0.013025610391785847 1106 7 -0.06477894108631688 1106 8 -0.05460795161533838 1106 9 -0.025514359771172224 1106 10-11 -0.008541466184261992 1106 12-13 -0.02181338491319451 1106 14-15 0.010471811084059368 1106 16-17 0.10420789722504509 1106 18-19 -0.023751210068546413 1106 20-21 -0.06795082813384568 1106 22-23 -0.15007453428341222 1106 24-25 -0.13253262879670302 1106 26-27 0.02355153504228724 1106 28-29 0.07770272059742211 1106 30-31 0.052520470358814464 1106 32-33 0.0273367447746935 1106 34-35 -0.06785728454688922 1106 36-37 -0.014390024565535953 1106 38-39 0.051587929487773465 1106 40-41 0.3168466232583498 1106 42-43 0.21145253844953515 1106 44-45 0.17375235835046965 1106 46-47 0.09453570739469086 1106 48-49 0.05326083592803599 1106 50-51 0.2090452800137399 1106 52-53 0.2572479437993209 1106 54-55 0.3528231754997222 1106 56-57 0.4050105319321631 1106 58-59 0.4038006081175709 1106 60-61 0.36755193985465695 1106 62-63 0.4800425562712576 1106 64-65 0.41789836372370814 1106 66-67 0.30895876061929073 1106 68-69 0.2244588559230536 1106 70-71 0.20477731393696175 1106 72-73 0.20207400973613687 1106 74-75 0.45214904487621155 1106 76-77 0.4237339170454675 1106 78-79 0.2944991952889673 1106 80-81 0.3316845256236789 1106 82-83 0.42327386757917296 1106 84-85 0.5519843598882872 1106 86-87 0.6948447197872589 1106 88-89 0.7804559811858844 1106 90-91 0.7723913872129842 1106 92-93 0.7090481405602596 1106 94-95 0.7297340017916305 1106 96-97 1.065068306488989 1106 98-99 0.8734541782843195 1106 100-101 1.548067043919577 1107 1 0.022961499710813627 1107 2 0.01763805775568983 1107 3 -0.05817906182465293 1107 4 -0.01415909371479529 1107 5 -0.10964025532565813 1107 6 -0.0832216790025484 1107 7 -0.026123050698622308 1107 8 -0.0014803736395663236 1107 9 -0.06501697853181554 1107 10-11 -0.1898663692532807 1107 12-13 0.015943877334152035 1107 14-15 0.04745674672290079 1107 16-17 -0.013075414894714754 1107 18-19 0.04188130836585202 1107 20-21 0.04058882492164173 1107 22-23 0.0871491402854474 1107 24-25 -0.04129918908699892 1107 26-27 -0.11982846815880066 1107 28-29 0.05097179984110056 1107 30-31 0.19810004377175972 1107 32-33 0.05825811807883241 1107 34-35 0.05376963887481878 1107 36-37 -0.14614393546707305 1107 38-39 -0.1703543485345591 1107 40-41 -0.2617517636388129 1107 42-43 -0.18998490363957643 1107 44-45 0.020637700876235954 1107 46-47 -0.11529360044024628 1107 48-49 -0.025304886779856872 1107 50-51 -0.10025809808041686 1107 52-53 -0.12692538545461218 1107 54-55 -0.07289083645754602 1107 56-57 -0.07334163956499395 1107 58-59 0.006458348024239058 1107 60-61 -0.030264858321665145 1107 62-63 0.05012502609045555 1107 64-65 0.016552199896828768 1107 66-67 -0.05233221524513709 1107 68-69 0.15736261887395386 1107 70-71 -0.08433560289892128 1107 72-73 -0.28129048160440817 1107 74-75 0.14084226317352844 1107 76-77 -0.01480986258594541 1107 78-79 0.03408757569320997 1107 80-81 0.10482882766435608 1107 82-83 0.17758617569974433 1107 84-85 0.10203007160649236 1107 86-87 0.2115858426586854 1107 88-89 0.27509034369523633 1107 90-91 0.6533491085010716 1107 92-93 0.5420036581043739 1107 94-95 0.694243271344142 1107 96-97 0.9615828923197718 1107 98-99 1.092509849313835 1107 100-101 1.6764202497827263 1108 1 0.10667535176930798 1108 2 0.09308809027414355 1108 3 0.09333526294501837 1108 4 0.05721379484260325 1108 5 -0.03634273976755509 1108 6 0.08117845885482922 1108 7 -0.004562172985259849 1108 8 0.08533888033375803 1108 9 0.0586213488682219 1108 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-1.1078069442100613 2316 56-57 -1.1440402697163634 2316 58-59 -1.2444646784455884 2316 60-61 -1.1882947948639213 2316 62-63 -1.2770528141343576 2316 64-65 -1.1308698884092152 2316 66-67 -1.1393166757808366 2316 68-69 -1.255620545977223 2316 70-71 -1.204707914449802 2316 72-73 -1.1563062751656759 2316 74-75 -1.4901508464588105 2316 76-77 -1.3323344681669056 2316 78-79 -1.1702356076447487 2316 80-81 -1.2473182391781954 2316 82-83 -1.3467558584129478 2316 84-85 -1.6829538478356731 2316 86-87 -2.026823433350465 2316 88-89 -2.2993299669820537 2316 90-91 -2.84699161242731 2316 92-93 -2.500163487998229 2316 94-95 -2.296594538144035 2316 96-97 -2.3489687465881417 2316 98-99 -2.464439895136721 2316 100-101 -2.434966269148372 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 26.0 5 69.0 6 194.0 7 435.0 8 857.0 9 1554.0 10 2404.0 11 3806.0 12 5403.0 13 7393.0 14 8524.0 15 10968.0 16 14104.0 17 19156.0 18 17653.0 19 11995.0 20 10651.0 21 10971.0 22 11804.0 23 13187.0 24 15017.0 25 17902.0 26 21331.0 27 25748.0 28 31016.0 29 37573.0 30 44817.0 31 54369.0 32 65920.0 33 83206.0 34 109913.0 35 154698.0 36 216043.0 37 318245.0 38 245722.0 39 19643.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41205547847449 20.207174009259674 14.92736974543357 21.453400766832267 2 15.743518529429823 21.979350342703434 39.68593219613639 22.59119893173035 3 19.279773892020664 27.024604810314536 30.465842226793942 23.22977907087086 4 12.883614800223528 18.015328156137063 37.114431501798336 31.98662554184108 5 12.741486402314795 38.47838440788837 34.04607752876744 14.7340516610294 6 29.54485826479113 36.96707803360113 18.411900758373147 15.07616294323459 7 26.90173891524486 34.15733905094934 21.397334411691922 17.543587622113876 8 25.973019120811102 34.838714479134815 20.35418373568141 18.834082664372673 9 26.69718983804154 17.012677949324175 21.101488717859343 35.188643494774944 10-11 24.35219406485161 27.51969668683834 27.32001634904086 20.80809289926919 12-13 25.758298409689846 25.256539151177147 28.08878099789931 20.896381441233693 14-15 22.70643610136157 26.150743614663774 27.400428076934762 23.742392207039885 16-17 20.880033517761653 29.48976898856557 28.086445309265546 21.543752184407232 18-19 20.639671729121947 27.451472312998543 31.360108712438606 20.548747245440907 20-21 21.806742676888746 26.52780732444473 31.274983300533947 20.390466698132574 22-23 21.383627514765926 26.733100667544484 30.991759115861733 20.891512701827857 24-25 21.10309192349999 26.88493894022471 31.37169043117235 20.640278705102947 26-27 21.078338490030884 26.946336749664844 31.25802229045444 20.717302469849834 28-29 20.63309738037011 27.002338855579545 31.60977890961325 20.754784854437098 30-31 20.832250529824282 27.060794739528642 31.410005538578712 20.69694919206836 32-33 20.673039650145412 27.18043548238043 31.143899469524484 21.00262539794967 34-35 21.184385138385228 27.09018400398705 30.994395617721025 20.7310352399067 36-37 21.321165306794686 26.864517545791383 30.91119383588114 20.903123311532784 38-39 21.09334025599584 27.00464891524794 31.095377975201856 20.806632853554362 40-41 21.0814353098581 26.984151598315698 31.190811952624443 20.743601139201758 42-43 20.988202616396006 27.1809993862346 30.698194586893084 21.132603410476314 44-45 21.01818323911741 27.497187167778776 30.314082024296418 21.170547568807397 46-47 21.159929145315367 27.57083917896886 29.774039051946456 21.495192623769316 48-49 21.414002582032886 27.643102644645477 29.515091991511483 21.42780278181016 50-51 21.36722928017371 27.90895640554193 29.388619488670564 21.335194825613797 52-53 21.49297257938371 27.938935464083897 29.309780212078568 21.258311744453824 54-55 21.08006006225784 27.869997835426684 29.47810834898876 21.571833753326715 56-57 21.276898601691368 28.33114702243062 28.961486815459853 21.43046756041816 58-59 21.324641937329535 27.81078227215709 29.220673903639003 21.64390188687437 60-61 21.32078256926996 27.76039382761917 29.495825474375092 21.422998128735784 62-63 21.39958227441811 27.681778078245245 29.46029048519121 21.45834916214544 64-65 21.188067473837048 27.64663998427091 29.429218773937095 21.736073767954952 66-67 20.991018651185005 28.086315227976154 29.131141338394645 21.791524782444192 68-69 21.034414961775624 27.975119113923895 29.2256757645185 21.76479015978198 70-71 20.929569786268477 28.055328743898954 29.038008120563667 21.9770933492689 72-73 21.10589229107991 27.89493074295723 28.859654400130992 22.139522565831868 74-75 20.974880009973194 28.0870471552975 28.75474897531293 22.183323859416376 76-77 21.080493880484198 27.883172541674888 29.002601461336152 22.03373211650476 78-79 20.960254298949707 27.855529576755345 28.995601753584545 22.188614370710404 80-81 20.80012007631296 28.26900939522721 28.75328411196868 22.177586416491142 82-83 20.976330131495136 28.18499376357918 28.539517696115436 22.299158408810253 84-85 20.92503567824641 27.889658859524154 28.537418278054016 22.64788718417542 86-87 21.04094262568627 27.717026028998376 28.72739013074259 22.514641214572762 88-89 21.134090078034433 27.591420102230558 28.63557218768721 22.6389176320478 90-91 20.896757006322726 27.8305251173467 28.795886008462475 22.476831867868096 92-93 20.888435159813387 27.84773227117727 28.6656230346619 22.598209534347443 94-95 20.64955347036543 27.756907269821244 28.590301074908687 23.003238184904635 96-97 20.906820614075755 27.538639065126734 28.40397301909571 23.1505673017018 98-99 20.790199506675755 27.527635523066973 28.30061767867188 23.38154729158539 100-101 20.95372419719525 26.97059264076393 29.011930468296697 23.063752693744124 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3645.0 1 2354.0 2 2368.0 3 3149.0 4 1978.5 5 1428.0 6 2190.5 7 4154.0 8 5742.5 9 5950.0 10 4738.0 11 3364.5 12 3093.5 13 3155.5 14 3321.0 15 3443.0 16 3520.5 17 3672.0 18 3850.5 19 4058.0 20 4363.5 21 5002.0 22 5794.5 23 6635.0 24 7655.5 25 9040.0 26 11213.5 27 13695.5 28 15823.0 29 18199.0 30 21270.5 31 24467.5 32 28087.0 33 31428.5 34 34587.5 35 37472.0 36 40784.5 37 44266.0 38 46221.5 39 46825.5 40 47140.5 41 49409.0 42 51607.5 43 52993.0 44 54484.0 45 55640.0 46 56009.0 47 57218.5 48 65126.0 49 66096.0 50 59145.0 51 55238.5 52 50882.0 53 48914.0 54 47157.5 55 42770.0 56 38236.0 57 33986.5 58 30107.5 59 26373.0 60 22581.5 61 18812.5 62 15130.0 63 11730.5 64 8694.0 65 6661.0 66 4977.5 67 3855.0 68 2856.0 69 1855.5 70 1374.5 71 1108.5 72 955.5 73 820.5 74 642.0 75 542.5 76 383.0 77 212.5 78 139.0 79 99.5 80 65.0 81 37.5 82 26.0 83 18.5 84 10.0 85 7.5 86 5.0 87 3.0 88 2.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2252024558293696 2 0.0 3 0.0 4 0.0 5 0.0269796387457383 6 0.0037213294821708005 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.00294605250671855 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.372991334884301E-4 26-27 0.00139549855581405 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006605359830853171 36-37 0.01786238151441984 38-39 0.01429610742733949 40-41 0.009024223994264191 42-43 0.009148268310336551 44-45 0.004527617536641141 46-47 8.062880544703401E-4 48-49 0.0019536979781396703 50-51 3.101107901809E-5 52-53 0.0 54-55 0.0 56-57 0.0013644874767959603 58-59 2.7909971116281E-4 60-61 0.00297706358573664 62-63 0.00151954287188641 64-65 0.0017366204250130403 66-67 0.006016149329509461 68-69 3.1011079018090004E-4 70-71 3.101107901809E-5 72-73 0.0 74-75 3.411218691989901E-4 76-77 0.057370496183466506 78-79 0.13976693313453165 80-81 0.0011784210026874202 82-83 0.00148853179286832 84-85 0.0 86-87 0.10664710074321153 88-89 0.35132451419594163 90-91 0.39046049591677123 92-93 5.5819942232562E-4 94-95 2.4808863214472E-4 96-97 9.303323705427E-5 98-99 0.0 100-101 0.00164358718795877 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1612327.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.774372244825436 #Duplication Level Percentage of deduplicated Percentage of total 1 80.37673238041765 47.24091988749381 2 12.055045731645903 14.170554905203007 3 3.407412527856434 6.00805596911747 4 1.3594854467376936 3.1961161483193603 5 0.6926291745562198 2.0354422466496715 6 0.42445560467846866 1.496826702646487 7 0.26405960459306543 1.0863956246621973 8 0.1856814305967237 0.8730647616674848 9 0.15249590390183113 0.8066565919563615 >10 0.9415415662988332 10.68182195977953 >50 0.09206470733765904 3.787049541323966 >100 0.045437355817248434 4.847145324420271 >500 0.0015849458369512116 0.6015898016999979 >1k 0.0010566305579674743 1.45873261609696 >5k 2.1132611159349488E-4 1.0369304138520559 >10k+ 1.0566305579674744E-4 0.6726975051114253 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10832 0.6718240158479019 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9916 0.6150117190867609 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6781 0.4205722536433366 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4174 0.25888048764301536 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3796 0.2354361119053393 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2656 0.16473085174409408 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2561 0.15883874673065698 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2530 0.15691605983153542 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2086 0.1293782216634715 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2004 0.12429240470450474 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.202215803618E-5 0.0 0.0 0.0 0.0 3 6.202215803618E-5 0.0 0.0 0.0 0.0 4 6.202215803618E-5 0.0 0.0 0.0 0.0 5 6.202215803618E-5 0.0 0.0 0.0 0.0 6 6.202215803618E-5 0.0 0.0 0.0 0.0 7 6.202215803618E-5 0.0 0.0 0.0 0.0 8 6.202215803618E-5 0.0 0.0 0.0 0.0 9 6.202215803618E-5 0.0 0.0 0.0 0.0 10-11 6.202215803618E-5 0.0 0.0 0.0 0.0 12-13 6.202215803618E-5 0.0 0.0 6.202215803618E-5 0.0 14-15 1.2404431607236E-4 0.0 0.0 9.303323705427E-5 0.0 16-17 1.2404431607236E-4 0.0 0.0 1.2404431607236E-4 0.0 18-19 1.2404431607236E-4 0.0 0.0 1.8606647410854003E-4 0.0 20-21 1.2404431607236E-4 0.0 0.0 2.4808863214472E-4 0.0 22-23 1.2404431607236E-4 0.0 0.0 6.512326593798901E-4 0.0 24-25 1.2404431607236E-4 0.0 0.0 0.0015505539509045002 0.0 26-27 1.2404431607236E-4 0.0 0.0 0.0026669527955557404 0.0 28-29 1.2404431607236E-4 0.0 0.0 0.00586109393441901 0.0 30-31 1.2404431607236E-4 0.0 0.0 0.01438914066439376 0.0 32-33 1.2404431607236E-4 0.0 0.0 0.03063894606987292 0.0 34-35 1.2404431607236E-4 0.0 0.0 0.060068460058040334 0.0 36-37 1.2404431607236E-4 0.0 0.0 0.11005831943520142 0.0 38-39 1.2404431607236E-4 0.0 0.0 0.17843774867008988 0.0 40-41 1.2404431607236E-4 0.0 0.0 0.2483987429349009 0.0 42-43 1.2404431607236E-4 0.0 0.0 0.3192280474122185 0.0 44-45 1.2404431607236E-4 0.0 0.0 0.4149282372620442 0.0 46-47 1.5505539509045002E-4 0.0 0.0 0.5328013486098043 0.0 48-49 1.8606647410854003E-4 0.0 0.0 0.6570007200772547 0.0 50-51 1.8606647410854003E-4 0.0 0.0 0.7889838723782459 0.0 52-53 1.8606647410854003E-4 0.0 0.0 0.9221144346029062 0.0 54-55 1.8606647410854003E-4 0.0 0.0 1.072611201077697 0.0 56-57 1.8606647410854003E-4 0.0 0.0 1.2509869275897507 0.0 58-59 1.8606647410854003E-4 0.0 0.0 1.4580479021935377 0.0 60-61 1.8606647410854003E-4 0.0 0.0 1.6877159534015123 0.0 62-63 1.8606647410854003E-4 0.0 0.0 1.9110887555688145 0.0 64-65 1.8606647410854003E-4 0.0 0.0 2.1361981781611297 0.0 66-67 1.8606647410854003E-4 0.0 0.0 2.401156837291691 0.0 68-69 1.8606647410854003E-4 0.0 0.0 2.6993903842086624 0.0 70-71 1.8606647410854003E-4 0.0 0.0 3.0291932095660496 0.0 72-73 1.8606647410854003E-4 0.0 0.0 3.361818043114083 0.0 74-75 1.8606647410854003E-4 0.0 0.0 3.706041020214882 0.0 76-77 1.8606647410854003E-4 0.0 0.0 4.079848566698939 0.0 78-79 1.8606647410854003E-4 0.0 0.0 4.49304018353597 0.0 80-81 1.8606647410854003E-4 0.0 0.0 4.943569139510782 0.0 82-83 1.8606647410854003E-4 0.0 0.0 5.402564120057532 0.0 84-85 1.8606647410854003E-4 0.0 0.0 5.8737464546583915 0.0 86-87 1.8606647410854003E-4 0.0 0.0 6.36589227867548 0.0 88-89 1.8606647410854003E-4 0.0 0.0 6.893979943274534 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1620 0.0 42.477158 2 GTACCTG 2035 0.0 33.42506 1 GTACCCG 160 1.879016E-9 29.729017 1 ACCTGGG 2255 0.0 28.62166 3 GGTATCA 9970 0.0 28.291767 1 GTATCAA 13860 0.0 25.945324 1 CGTATCA 95 0.0012332266 24.977493 2 GCGTATC 115 1.2699577E-4 24.817266 1 TATCACG 115 1.2899289E-4 24.760298 2 ATGGGCG 230 1.364242E-10 24.760298 5 GGATCGC 100 0.0016653298 23.721996 8 GTACATG 10235 0.0 23.65541 1 TACATGG 10425 0.0 23.079924 2 GTACACG 325 1.8189894E-12 21.953735 1 ACATGGG 10310 0.0 21.818361 3 GAGTACT 9215 0.0 21.548733 12-13 ATCAACG 16655 0.0 21.370716 3 TATCAAC 16785 0.0 21.318295 2 TCAACGC 16845 0.0 21.186014 4 CAACGCA 17080 0.0 20.922306 5 >>END_MODULE