FastQCFastQC Report
Thu 26 May 2016
SRR938713_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938713_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2904070
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA193180.6652043511347867No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182450.6282562059454491No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA160790.5536712269332351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133840.4608704335639292No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT116320.4005413092659612No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT109610.3774358056107463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93850.32316714128791657No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA72050.24810008023222582No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT65360.22506344544036472No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG64330.22151669897764173No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT63760.21955393637205714No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG51110.17599438030075032No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC50600.17423822428522728No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50560.1741004865585196No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46210.15912150877905837No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC45950.15822621355545838No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG35150.1210370273443822No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34940.12031390427916681No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32600.11225624726676699No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT29960.10316555730405948No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG227550.023.7100181
TACCTGG24050.023.6412532
GAGTACT135950.023.12604112-13
TACATGG231200.022.9527382
CATGGGG63550.022.3671174
AGTACTT140100.022.13683912-13
ACATGGG231200.021.743623
AGTTCGC1100.002932311521.5219828
GTACTTT149000.021.19590614-15
ATGGGAT55950.019.3080795
CATGGGA158050.018.9763874
ACTTTTT169950.018.89049716-17
TACTTTT163850.018.75472314-15
ATGGGAG46050.018.7260465
ATGGGGA33950.018.5616325
GTATAGG7550.017.5823751
TACACCG3852.6011548E-1017.2294585
ACCTGGG33500.016.8308663
TCCCGTA1700.00181870416.722712
GTACCTG35250.016.6774251
Produced by FastQC (version 0.11.5)