##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938713_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2904070 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.559307454710115 33.0 31.0 34.0 30.0 34.0 2 31.59754861280892 33.0 31.0 34.0 30.0 34.0 3 31.60428467633356 33.0 31.0 34.0 28.0 34.0 4 35.15128664253961 37.0 35.0 37.0 32.0 37.0 5 35.19369953203607 37.0 35.0 37.0 33.0 37.0 6 35.30034262259519 37.0 35.0 37.0 33.0 37.0 7 35.2171727954216 37.0 35.0 37.0 32.0 37.0 8 35.19528179417163 37.0 35.0 37.0 32.0 37.0 9 36.72902857024796 39.0 37.0 39.0 32.0 39.0 10-11 36.62630274063642 39.0 37.0 39.0 32.0 39.0 12-13 36.50368551722238 39.0 37.0 39.0 32.0 39.0 14-15 37.58548898614703 40.0 37.0 41.0 32.0 41.0 16-17 37.540244381161614 40.0 37.0 41.0 32.0 41.0 18-19 37.5306576632106 40.0 37.0 41.0 32.0 41.0 20-21 37.433653286594335 40.0 37.0 41.0 32.0 41.0 22-23 37.34146336004298 40.0 37.0 41.0 31.5 41.0 24-25 37.334790139356144 40.0 37.0 41.0 31.0 41.0 26-27 37.20498128488639 40.0 37.0 41.0 31.0 41.0 28-29 37.0749172712779 40.0 36.5 41.0 31.0 41.0 30-31 36.868829608101734 39.0 36.0 41.0 30.0 41.0 32-33 36.64558550585902 39.0 35.5 41.0 30.0 41.0 34-35 36.30684556501737 39.0 35.0 41.0 30.0 41.0 36-37 36.02346723735998 39.0 35.0 41.0 29.0 41.0 38-39 35.81599548220257 39.0 35.0 40.5 28.5 41.0 40-41 35.61510380259429 39.0 35.0 40.0 27.0 41.0 42-43 35.34320935790115 38.0 35.0 40.0 26.5 41.0 44-45 35.252683303088425 39.0 35.0 40.5 25.5 41.0 46-47 34.98760360459631 39.0 35.0 41.0 24.0 41.0 48-49 34.70097862654826 39.0 34.0 41.0 23.0 41.0 50-51 33.528201627371246 38.0 32.5 39.5 21.5 40.5 52-53 33.598502274394214 37.5 33.0 39.5 21.5 40.5 54-55 34.11367339630243 38.0 34.0 40.0 22.0 41.0 56-57 34.031475480962925 38.0 34.0 40.0 20.5 41.0 58-59 33.818529339857506 38.0 33.5 40.0 20.0 41.0 60-61 33.50051789385242 37.5 33.0 40.0 18.5 41.0 62-63 33.116803658314026 37.0 33.0 40.0 17.0 41.0 64-65 32.680813479013935 36.0 32.0 39.5 13.0 41.0 66-67 32.26765763221961 36.0 32.0 39.0 9.5 41.0 68-69 31.8793507043563 35.0 31.0 39.0 8.0 40.0 70-71 31.445398354722855 35.0 31.0 38.0 7.0 40.0 72-73 31.01443491375897 35.0 31.0 37.0 2.0 39.5 74-75 30.574842032044682 35.0 30.0 37.0 2.0 39.0 76-77 30.127902392159967 35.0 30.0 36.0 2.0 39.0 78-79 29.70927353679491 34.0 29.5 36.0 2.0 38.0 80-81 29.29141497966647 34.0 29.0 35.0 2.0 37.0 82-83 28.855337164737765 34.0 29.0 35.0 2.0 37.0 84-85 28.461337708801786 34.0 29.0 35.0 2.0 36.0 86-87 28.076764678537366 34.0 28.0 35.0 2.0 36.0 88-89 27.68651633741611 34.0 27.0 35.0 2.0 35.5 90-91 27.24645600829181 34.0 26.0 35.0 2.0 35.0 92-93 26.82656685272738 33.0 25.0 35.0 2.0 35.0 94-95 26.351906462309792 33.0 24.5 35.0 2.0 35.0 96-97 25.756373468959083 33.0 22.5 35.0 2.0 35.0 98-99 25.062674453439485 33.0 17.0 35.0 2.0 35.0 100-101 22.916805207863447 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2019067771297003 1101 2 -0.07327332497884242 1101 3 0.15987287818848728 1101 4 0.13241748924126995 1101 5 0.0157707279822219 1101 6 -0.04985141117245462 1101 7 0.16904298204858748 1101 8 0.19921642271588524 1101 9 0.25811471757244675 1101 10-11 0.193163267013162 1101 12-13 0.25384393157965945 1101 14-15 0.3077935545607815 1101 16-17 0.2796883642666117 1101 18-19 0.30494693728331157 1101 20-21 0.27566063766250437 1101 22-23 0.27039920145710994 1101 24-25 0.3095980611172493 1101 26-27 0.35366284847550844 1101 28-29 0.2165689803883808 1101 30-31 0.26694927004368907 1101 32-33 0.16924316438345954 1101 34-35 0.19605541636600776 1101 36-37 0.19423070850017865 1101 38-39 0.31427114894673736 1101 40-41 0.34104333793086283 1101 42-43 0.1297097197005499 1101 44-45 0.16280599807372198 1101 46-47 0.2046086519664314 1101 48-49 0.3231865377055101 1101 50-51 0.2874270240292063 1101 52-53 0.2028638708579038 1101 54-55 0.16935473465085948 1101 56-57 0.09590415422827192 1101 58-59 0.043000453415757534 1101 60-61 0.058446985574313715 1101 62-63 0.11596630438931044 1101 64-65 -0.10576280137296834 1101 66-67 -0.15527987996484427 1101 68-69 -0.0687813279375149 1101 70-71 -0.3113269288793923 1101 72-73 -0.20117007674646104 1101 74-75 -0.29013649790235263 1101 76-77 -0.3425323603934771 1101 78-79 -0.3820344971964431 1101 80-81 -0.33532577118945284 1101 82-83 -0.39038435634911295 1101 84-85 -0.42461121825899895 1101 86-87 -0.5718376887102998 1101 88-89 -0.5068003832737595 1101 90-91 -0.491219759643748 1101 92-93 -0.7020871463433274 1101 94-95 -0.6666779999073214 1101 96-97 -0.8766873415200571 1101 98-99 -0.8908126169376551 1101 100-101 -0.55486659263671 1102 1 0.370593240182604 1102 2 0.35641512640483697 1102 3 0.3776452958031449 1102 4 0.3472872046817983 1102 5 0.22670889415451967 1102 6 0.2748392241155244 1102 7 0.3437246861458547 1102 8 0.25888707690930346 1102 9 0.3902727409760729 1102 10-11 0.339401019221512 1102 12-13 0.44679494041306356 1102 14-15 0.2738670250128834 1102 16-17 0.4848213868553515 1102 18-19 0.5142529120543315 1102 20-21 0.48183724055046895 1102 22-23 0.45237077810563164 1102 24-25 0.5979425312759901 1102 26-27 0.5452490800997509 1102 28-29 0.49092016523795223 1102 30-31 0.468529373697649 1102 32-33 0.45855187718374424 1102 34-35 0.4540765383060972 1102 36-37 0.5769214741705042 1102 38-39 0.4441982000240188 1102 40-41 0.478578329293029 1102 42-43 0.46332665636732884 1102 44-45 0.5580325699958806 1102 46-47 0.5727602214439216 1102 48-49 0.40848253822703384 1102 50-51 0.3418718234033662 1102 52-53 0.315079914405608 1102 54-55 0.37335356120974694 1102 56-57 0.39640886429052813 1102 58-59 0.3793049613853796 1102 60-61 0.38898976728166446 1102 62-63 0.37950233881024786 1102 64-65 0.4292140883561615 1102 66-67 0.28184303799206845 1102 68-69 0.35746222628524293 1102 70-71 0.293380721304775 1102 72-73 0.23009065535040918 1102 74-75 0.10763774506515134 1102 76-77 0.16091410135560125 1102 78-79 0.11914631052218994 1102 80-81 0.07074007189539344 1102 82-83 0.06288315562976621 1102 84-85 -0.010879545127540524 1102 86-87 -0.015887038428349598 1102 88-89 0.028436097706716623 1102 90-91 0.03409442921508088 1102 92-93 -0.2353405118376415 1102 94-95 -0.4051930101749299 1102 96-97 -0.36453114346927507 1102 98-99 -0.3953206517497456 1102 100-101 -0.18829630025429722 1103 1 0.13040708219376285 1103 2 0.1800437810871145 1103 3 0.12362356056862822 1103 4 0.1863352182801563 1103 5 0.09028304293678246 1103 6 0.18763409025653033 1103 7 0.16486007205854492 1103 8 0.12241233806863505 1103 9 0.27088257987615805 1103 10-11 0.153306229666903 1103 12-13 0.21142320006814685 1103 14-15 0.28323660391694006 1103 16-17 0.30659193191055323 1103 18-19 0.3986992894878014 1103 20-21 0.24804649553475855 1103 22-23 0.39477780201585944 1103 24-25 0.2837633095036267 1103 26-27 0.21480237514453648 1103 28-29 0.26737916631689984 1103 30-31 0.38700976906427087 1103 32-33 0.2892283286363764 1103 34-35 0.3103603519814371 1103 36-37 0.3776875660192047 1103 38-39 0.30232638159822756 1103 40-41 0.43506549521546845 1103 42-43 0.30874012977087517 1103 44-45 0.3541557941624802 1103 46-47 0.3004545785583872 1103 48-49 0.33540143125421196 1103 50-51 0.34680687695621515 1103 52-53 0.3513627629936664 1103 54-55 0.4388361257429949 1103 56-57 0.41387840039890733 1103 58-59 0.4189001997308992 1103 60-61 0.3591932043855266 1103 62-63 0.4621895470597437 1103 64-65 0.16701019885180557 1103 66-67 0.09969028798095536 1103 68-69 0.07958751057120494 1103 70-71 0.1522983240026612 1103 72-73 0.14306074435754468 1103 74-75 0.11901747916967409 1103 76-77 0.05071082383310355 1103 78-79 0.2020461039403223 1103 80-81 0.05466896244145403 1103 82-83 0.16996136151479746 1103 84-85 0.2243096898685515 1103 86-87 0.19219654268881925 1103 88-89 0.14400016905275592 1103 90-91 0.10339846070884207 1103 92-93 0.06056594764851653 1103 94-95 0.028281763748310595 1103 96-97 0.07688704594341189 1103 98-99 0.18996969576906508 1103 100-101 0.1646764535082461 1104 1 0.1066524439142782 1104 2 0.11256183596915648 1104 3 0.10098359626418585 1104 4 0.10653988046872342 1104 5 0.04931724372328716 1104 6 0.06631130548831976 1104 7 0.05506349849711967 1104 8 0.10698720446331578 1104 9 -0.021974422859109666 1104 10-11 0.07928424331312556 1104 12-13 0.18542228318914056 1104 14-15 0.22296299212710835 1104 16-17 0.1767835181399704 1104 18-19 0.07603784637422706 1104 20-21 0.09624641764127517 1104 22-23 0.15797543067536424 1104 24-25 0.12408691856330734 1104 26-27 0.12064622307756423 1104 28-29 0.06186788381958053 1104 30-31 0.11416823007198218 1104 32-33 0.11905780888292838 1104 34-35 0.03273387409505091 1104 36-37 0.09232409371418981 1104 38-39 0.1370217679775081 1104 40-41 0.019914225797862173 1104 42-43 0.08087420502067744 1104 44-45 0.11347030652791545 1104 46-47 0.019708050622249118 1104 48-49 0.05765700109425609 1104 50-51 0.11447194797685256 1104 52-53 0.06730957301567031 1104 54-55 0.12380821890979377 1104 56-57 0.0741114411048116 1104 58-59 -0.07591783452198797 1104 60-61 -0.08573907738995956 1104 62-63 -0.09935523788164602 1104 64-65 -0.05792834788871204 1104 66-67 -0.23171355523898995 1104 68-69 -0.1738616250722842 1104 70-71 -0.12889396106899298 1104 72-73 0.006443648050140638 1104 74-75 -0.04531583996107358 1104 76-77 -0.3352660002944319 1104 78-79 -0.2580734077022768 1104 80-81 -0.3639702706588537 1104 82-83 -0.327306181605568 1104 84-85 -0.22144107322892737 1104 86-87 -0.2964114418054571 1104 88-89 -0.3797186712115028 1104 90-91 -0.4454178494916441 1104 92-93 -0.3391016493500487 1104 94-95 -0.29074096418040085 1104 96-97 -0.19885925520948078 1104 98-99 -0.13433083412902747 1104 100-101 0.153350598732235 1105 1 0.11403813644924199 1105 2 0.09221908061064354 1105 3 0.16126321490066786 1105 4 0.2266954926307747 1105 5 0.12103610066792214 1105 6 0.08138665361186526 1105 7 0.2528603324786687 1105 8 0.0702915354329221 1105 9 0.16702054688207113 1105 10-11 0.2302603155854115 1105 12-13 0.24044668795097124 1105 14-15 0.26377846166915475 1105 16-17 0.22548224397114325 1105 18-19 0.2054146250651101 1105 20-21 0.19736639022861624 1105 22-23 0.1440992622123929 1105 24-25 0.13294215986057623 1105 26-27 0.10683133247129462 1105 28-29 0.151987392437114 1105 30-31 0.11000276667017062 1105 32-33 0.20840391007462955 1105 34-35 0.05132066114585143 1105 36-37 0.018970579954789457 1105 38-39 0.10058471016567694 1105 40-41 0.10738273613378624 1105 42-43 0.1777274346623372 1105 44-45 0.24362242118630206 1105 46-47 0.24576281050574522 1105 48-49 0.28371325404228287 1105 50-51 0.3745706942878968 1105 52-53 0.21859987316020835 1105 54-55 0.273782196039285 1105 56-57 0.31031300531386563 1105 58-59 0.4027383103530511 1105 60-61 0.3973468033084586 1105 62-63 0.27258747920465254 1105 64-65 0.37285044327896344 1105 66-67 0.2685738596827747 1105 68-69 0.2267919141563084 1105 70-71 0.338823744225202 1105 72-73 0.2607707667926924 1105 74-75 0.34033681773733804 1105 76-77 0.3751812583831047 1105 78-79 0.2781184142607245 1105 80-81 0.29366978408187094 1105 82-83 0.2849195160008371 1105 84-85 0.15006143866550659 1105 86-87 0.1925075931622402 1105 88-89 0.19365176394588346 1105 90-91 0.3629873199456739 1105 92-93 0.39721065047821114 1105 94-95 0.4625525184311847 1105 96-97 0.4844158465335333 1105 98-99 0.33836673905632964 1105 100-101 0.46209276527586596 1106 1 0.18661072512702503 1106 2 0.22183944308029524 1106 3 0.19986523073604445 1106 4 0.19166217585504342 1106 5 0.2653467404730634 1106 6 0.2156352535823487 1106 7 0.1841983209263205 1106 8 0.243806551129353 1106 9 0.2643936584002802 1106 10-11 0.27236716721390763 1106 12-13 0.26085371394591306 1106 14-15 0.30660297367304423 1106 16-17 0.36708128400022133 1106 18-19 0.36413968494925086 1106 20-21 0.3646828471389725 1106 22-23 0.2577892533960622 1106 24-25 0.1920101950751274 1106 26-27 0.17326256806892104 1106 28-29 0.2541096871404491 1106 30-31 0.1583124921703174 1106 32-33 0.33090247037714704 1106 34-35 0.23690269036785594 1106 36-37 0.3071386522914068 1106 38-39 0.33886293430823855 1106 40-41 0.40140952344530945 1106 42-43 0.4254500249613571 1106 44-45 0.30343665798513086 1106 46-47 0.4310845784234303 1106 48-49 0.34618280957243996 1106 50-51 0.3623027210626333 1106 52-53 0.3603361329106036 1106 54-55 0.34479876485830374 1106 56-57 0.501371063064262 1106 58-59 0.3676132055427104 1106 60-61 0.5040511024528698 1106 62-63 0.3295849514275204 1106 64-65 0.2599674347421228 1106 66-67 0.27181880403605163 1106 68-69 0.3946957775017381 1106 70-71 0.3753506354070062 1106 72-73 0.21813642677455292 1106 74-75 0.16769838085366118 1106 76-77 -0.03170745519002338 1106 78-79 0.14435883677418104 1106 80-81 0.05018670371734402 1106 82-83 0.10065340294732295 1106 84-85 0.3453246705980284 1106 86-87 0.4170024476692653 1106 88-89 0.3930511286913152 1106 90-91 0.502021103243802 1106 92-93 0.656774020530456 1106 94-95 0.6224194732469357 1106 96-97 0.6159481617779043 1106 98-99 0.7828146972133148 1106 100-101 1.1223876532926162 1107 1 0.03939038343039414 1107 2 0.16381445255536775 1107 3 0.09574110228268395 1107 4 0.16031577627975935 1107 5 0.10012998016648567 1107 6 0.03835101265242713 1107 7 -0.10172330987512623 1107 8 0.09448376371108935 1107 9 0.06293268018165321 1107 10-11 0.07396857813331792 1107 12-13 0.055922909439551916 1107 14-15 0.07985493307204194 1107 16-17 0.08627878796622923 1107 18-19 0.16409722068919308 1107 20-21 -0.007896059171493164 1107 22-23 0.17291265149312807 1107 24-25 0.12247940643400312 1107 26-27 0.1363421457541918 1107 28-29 0.0281945714697045 1107 30-31 0.13909883319152527 1107 32-33 0.22922042928183828 1107 34-35 0.24966484861477767 1107 36-37 0.3372141425652586 1107 38-39 0.37386980864047104 1107 40-41 0.32255688191357734 1107 42-43 0.3933716994706984 1107 44-45 0.35054632939241515 1107 46-47 0.41260332832004565 1107 48-49 0.4824228136582889 1107 50-51 0.5890029486213209 1107 52-53 0.4567126166694422 1107 54-55 0.5115230245077527 1107 56-57 0.5915847354451884 1107 58-59 0.5330271342788109 1107 60-61 0.43491628066090016 1107 62-63 0.5132635830665393 1107 64-65 0.39512693651573016 1107 66-67 0.2534333007572869 1107 68-69 0.4047727383888571 1107 70-71 0.5657501387309161 1107 72-73 0.4435766116323272 1107 74-75 0.4766700032093496 1107 76-77 0.4245712001864881 1107 78-79 0.4678543951719547 1107 80-81 0.4621357263209589 1107 82-83 0.5667771380297459 1107 84-85 0.5586098383282341 1107 86-87 0.5252333663111486 1107 88-89 0.5260039257482525 1107 90-91 0.6061772277987103 1107 92-93 0.5175019934180476 1107 94-95 0.3886987576788776 1107 96-97 0.34751270232173326 1107 98-99 0.47376465422383873 1107 100-101 0.5633424700883722 1108 1 0.09569970469478051 1108 2 0.005135657075708622 1108 3 0.0935425202125586 1108 4 0.021221519969351732 1108 5 0.18834572875975653 1108 6 0.13205037093185723 1108 7 0.11151864528374489 1108 8 0.10358155194177243 1108 9 0.022800190156651468 1108 10-11 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2316 46-47 -2.5279801788978986 2316 48-49 -2.23874013819772 2316 50-51 -2.569766943784927 2316 52-53 -2.464824821457988 2316 54-55 -2.2097393717117058 2316 56-57 -2.358666025884702 2316 58-59 -1.954327480080991 2316 60-61 -2.062554399910077 2316 62-63 -2.256596415423111 2316 64-65 -2.354594518307856 2316 66-67 -2.32205617752426 2316 68-69 -2.5270647127824475 2316 70-71 -2.2911972262256555 2316 72-73 -2.5135332868072133 2316 74-75 -2.5423365405326415 2316 76-77 -2.4662941636546947 2316 78-79 -2.4365957249487593 2316 80-81 -2.27164272738683 2316 82-83 -2.1649246952599093 2316 84-85 -2.152827353823316 2316 86-87 -2.276167860297214 2316 88-89 -2.430487903256708 2316 90-91 -2.552913461018573 2316 92-93 -2.7835626872151202 2316 94-95 -2.9056878048737467 2316 96-97 -3.0499656107303323 2316 98-99 -3.2674303298226626 2316 100-101 -3.33341343266655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11014.0 3 3282.0 4 3241.0 5 3807.0 6 4386.0 7 5195.0 8 6315.0 9 7657.0 10 9421.0 11 12333.0 12 15386.0 13 19843.0 14 21167.0 15 21599.0 16 27088.0 17 34291.0 18 28915.0 19 20410.0 20 20433.0 21 22031.0 22 23719.0 23 26131.0 24 29821.0 25 34212.0 26 39195.0 27 45548.0 28 53415.0 29 62646.0 30 74864.0 31 90148.0 32 110000.0 33 139774.0 34 185109.0 35 260644.0 36 375585.0 37 534387.0 38 450612.0 39 70420.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.85117761017925 18.452956812625004 14.112145696903728 21.583719880292023 2 14.964776778282406 21.248638135380286 40.08609086863956 23.70049421769774 3 20.357725796401375 26.57071112287493 29.52537124959332 23.546191831130376 4 12.47491463565124 16.348246558022595 37.69160875173297 33.485230054593195 5 13.284345937939799 38.7524590808261 32.94389123984563 15.019303741388477 6 30.165323989133647 35.859721258628426 18.120290495110762 15.854664257127169 7 28.071871907114126 34.228206878044226 20.311826627178778 17.388094587662874 8 26.15960522435571 32.99579841196784 19.970634358489576 20.873962005186876 9 27.342543414055037 17.81868467479637 20.769052420014685 34.06971949113391 10-11 24.825690494391676 26.466812896371323 26.65759822016378 22.049898389073217 12-13 27.72937419824603 24.13606945861507 26.86100370337769 21.27355263976121 14-15 24.292544401040274 24.891402495132965 27.1284645868265 23.68758851700026 16-17 21.577821656110203 28.702544351881947 27.59673802895798 22.122895963049874 18-19 21.283491234516287 27.722083582462865 30.121427069084287 20.87299811393656 20-21 22.932963405613478 26.228239385450514 29.089465845365574 21.749331363570434 22-23 22.150743338279042 26.255333063650816 28.865975829187597 22.727947768882544 24-25 22.40482091803116 26.315872002779074 29.513734764040795 21.765572315148976 26-27 22.679733764262195 25.720877062928913 30.061113736064343 21.538275436744545 28-29 21.537528998082646 26.41408297138856 30.476709181106553 21.571678849422245 30-31 21.603038192934005 26.85401316874158 29.88033600809578 21.662612630228633 32-33 21.457443500744596 26.89735778397341 29.455562417261184 22.189636298020815 34-35 22.29884382582266 26.174263683696996 29.36357749130063 22.16331499917971 36-37 22.734788053718237 25.851818648449417 29.741299297321298 21.672094000511045 38-39 22.822538241744557 25.752381677171183 30.025771234459427 21.399308846624834 40-41 21.918387377236613 26.315718486148203 30.30288725238956 21.463006884225628 42-43 21.592774231579735 26.932810545155085 29.249240959097673 22.22517426416751 44-45 21.78493849486666 27.002214423524194 28.59538498291827 22.617462098690876 46-47 22.124263355182205 26.82325592901016 28.044044643871896 23.008436071935733 48-49 22.582363605094848 26.70046600244507 28.04554761256362 22.67162277989646 50-51 22.592407293421505 27.18008415273434 28.258496495596702 21.96901205824745 52-53 22.200348162596292 27.43572815359292 28.27374742606389 22.090176257746894 54-55 21.30578336830101 26.90264776382146 28.783757881567514 23.007810986310016 56-57 22.2585599468684 27.61440502813104 27.584155299237096 22.542879725763466 58-59 22.49522399167778 26.681580308873897 27.87433737639787 22.948858323050448 60-61 22.16802994386994 26.441769575815343 28.7468688185582 22.64333166175652 62-63 23.004326898795263 26.41611842446642 28.294624862579106 22.284929814159213 64-65 22.44961346008165 26.800597035379504 28.54915419411125 22.200635310427597 66-67 21.913904691503376 26.957971188282205 28.37383007443884 22.754294045775584 68-69 21.6665772546115 27.48849612961172 28.285555569015436 22.55937104676135 70-71 22.024699912297763 27.320267522969054 27.643647886943718 23.011384677789458 72-73 21.936875946813213 26.937451280485114 27.614060981908565 23.511611790793104 74-75 21.867727762373253 27.624320767598793 27.621085297713403 22.88686617231455 76-77 22.52515287373561 26.666708183763667 27.95788990046449 22.850249042036232 78-79 22.024455071937922 26.745380755712674 28.38494247162493 22.845221700724473 80-81 21.998616395710826 27.691819439640263 27.82011414735386 22.489450017295056 82-83 21.933713411955182 27.331239210829473 27.651705683536115 23.083341693679234 84-85 21.88911205651776 26.877744575539094 27.610801805236235 23.622341562706914 86-87 22.463871407466865 26.80909364545024 27.854444240702747 22.87259070638015 88-89 22.73094364979789 26.29154189881503 27.769638696292155 23.20787575509492 90-91 22.10849838687939 26.561894712109833 28.316577537146237 23.01302936386454 92-93 22.170525843354493 27.30312212408734 27.850677727011863 22.675674305546302 94-95 21.92377482273768 27.13100204189722 27.577568649082806 23.367654486282298 96-97 21.896467147980083 26.692615487102735 27.401296864253176 24.009620500664006 98-99 22.407564757569922 26.753791557384236 27.057334150669867 23.781309534375975 100-101 22.970544050718928 26.22667961119365 27.706448713308944 23.096327624778482 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4719.0 1 2920.0 2 1504.5 3 1921.5 4 1622.5 5 1544.0 6 2701.5 7 5153.5 8 7776.0 9 8528.5 10 7079.0 11 5718.5 12 5562.0 13 5823.0 14 6098.5 15 6253.5 16 6478.5 17 6635.5 18 6650.5 19 6774.0 20 7081.0 21 7689.0 22 8533.5 23 9675.5 24 10897.0 25 12553.0 26 15305.5 27 18540.5 28 21767.5 29 25562.0 30 29681.0 31 33936.5 32 39183.0 33 44531.5 34 50308.0 35 54998.5 36 60827.0 37 67836.5 38 70226.5 39 70202.5 40 71510.0 41 76003.0 42 80360.0 43 84986.0 44 91084.5 45 95651.0 46 99202.5 47 112875.5 48 174382.5 49 176514.5 50 120615.0 51 104793.5 52 93662.0 53 89997.5 54 88608.5 55 83756.0 56 77230.5 57 69040.5 58 62170.5 59 56309.0 60 49258.0 61 41771.5 62 34039.5 63 26349.0 64 19587.0 65 15002.0 66 11408.5 67 9098.5 68 7142.5 69 4983.0 70 3694.0 71 3103.0 72 2674.5 73 2233.5 74 1795.0 75 1469.0 76 1061.0 77 648.5 78 432.5 79 306.0 80 205.5 81 144.0 82 115.0 83 85.5 84 63.5 85 44.5 86 32.5 87 26.5 88 19.5 89 15.5 90 15.0 91 12.0 92 9.0 93 11.0 94 7.0 95 4.5 96 4.5 97 1.5 98 2.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16118757467967368 2 0.09355835086619813 3 0.08670589896249058 4 0.10247686867052101 5 0.1238262163102129 6 0.04008167847193766 7 0.04266426084770684 8 0.046761958217260605 9 0.04755395014582982 10-11 0.0458322285619837 12-13 0.049912708715699 14-15 0.04748508128247597 16-17 0.046365962252976 18-19 0.05039479075917592 20-21 0.052374770580598945 22-23 0.06514994473273716 24-25 0.08202281625442913 26-27 0.08371010340659832 28-29 0.07417176583209083 30-31 0.06261901400448336 32-33 0.051221217119422055 34-35 0.3011291050146863 36-37 0.47917233400021353 38-39 0.45625621971922165 40-41 0.4315322977751914 42-43 0.4619551181617523 44-45 0.4718033656213521 46-47 0.4712179802828444 48-49 0.4879703312936671 50-51 0.4768824442936982 52-53 0.4834938551756673 54-55 0.47457533737134433 56-57 0.4522962600763756 58-59 0.4316011666385452 60-61 0.3991467147830458 62-63 0.45852889220989856 64-65 0.49788744761662085 66-67 0.49986742743804385 68-69 0.5104904496103745 70-71 0.5078389983712515 72-73 0.49731927949395155 74-75 0.4898986594675748 76-77 0.4715623245996136 78-79 0.4452888532301219 80-81 0.45005802201737566 82-83 0.4627987617378369 84-85 0.4603194826570985 86-87 0.4717517139738367 88-89 0.4833216830172827 90-91 0.48809085180453643 92-93 0.48988144225173635 94-95 0.4943234839380593 96-97 0.49611407438525934 98-99 0.48881397486975176 100-101 0.4747819439614059 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2904070.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.614354946067564 #Duplication Level Percentage of deduplicated Percentage of total 1 68.22929269162063 26.346301257133725 2 14.456024396716499 11.164201143276463 3 6.218538587442888 7.20374568784012 4 3.3266526214546475 5.138261804284635 5 1.9552308969636427 3.7749989928436074 6 1.2975249789056675 3.0061854052111365 7 0.8909310288680367 2.4081908886822876 8 0.6228485174894943 1.9240714985577043 9 0.46533748753574056 1.6171836222074742 >10 2.2546676809441006 15.413300310124287 >50 0.16285147937423114 4.358368204974691 >100 0.10845562316623462 7.889069701476981 >500 0.005822004759142735 1.5406950829182016 >1k 0.004568034503320059 3.3462890038889443 >5k 7.165544318933426E-4 1.769520087823032 >10k+ 5.374158239200069E-4 3.099617308756739 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19318 0.6652043511347867 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18245 0.6282562059454491 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16079 0.5536712269332351 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13384 0.4608704335639292 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11632 0.4005413092659612 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10961 0.3774358056107463 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9385 0.32316714128791657 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7205 0.24810008023222582 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6536 0.22506344544036472 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6433 0.22151669897764173 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6376 0.21955393637205714 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5111 0.17599438030075032 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5060 0.17423822428522728 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5056 0.1741004865585196 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4621 0.15912150877905837 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4595 0.15822621355545838 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3515 0.1210370273443822 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3494 0.12031390427916681 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3260 0.11225624726676699 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2996 0.10316555730405948 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.443443167692239E-5 2 3.443443167692239E-5 0.0 0.0 0.0 3.443443167692239E-5 3 6.886886335384477E-5 0.0 0.0 0.0 3.443443167692239E-5 4 6.886886335384477E-5 0.0 0.0 3.443443167692239E-5 3.443443167692239E-5 5 1.0330329503076716E-4 0.0 0.0 3.443443167692239E-5 3.443443167692239E-5 6 1.0330329503076716E-4 0.0 0.0 3.443443167692239E-5 3.443443167692239E-5 7 1.7217215838461194E-4 0.0 0.0 3.443443167692239E-5 3.443443167692239E-5 8 1.7217215838461194E-4 0.0 0.0 3.443443167692239E-5 3.443443167692239E-5 9 1.7217215838461194E-4 0.0 0.0 6.886886335384477E-5 3.443443167692239E-5 10-11 1.8938937422307314E-4 0.0 0.0 1.2052051086922835E-4 3.443443167692239E-5 12-13 2.410410217384567E-4 0.0 0.0 1.7217215838461194E-4 3.443443167692239E-5 14-15 2.582582375769179E-4 0.0 0.0 2.926926692538403E-4 3.443443167692239E-5 16-17 2.926926692538403E-4 0.0 0.0 3.099098850923015E-4 3.443443167692239E-5 18-19 3.615615326076851E-4 0.0 0.0 3.9599596428460746E-4 3.443443167692239E-5 20-21 4.1321318012306864E-4 0.0 0.0 7.747747127307537E-4 3.443443167692239E-5 22-23 4.1321318012306864E-4 0.0 0.0 0.0018250248788768866 3.443443167692239E-5 24-25 4.4764761179999106E-4 0.0 0.0 0.0037017014052691567 3.443443167692239E-5 26-27 4.4764761179999106E-4 0.0 0.0 0.005802201737561422 3.443443167692239E-5 28-29 5.853853385076806E-4 0.0 0.0 0.011811010065184379 6.886886335384477E-5 30-31 6.542542018615254E-4 0.0 0.0 0.027392590398991758 1.0330329503076716E-4 32-33 6.886886335384477E-4 0.0 0.0 0.05457857420792199 1.0330329503076716E-4 34-35 8.264263602461373E-4 0.0 0.0 0.09402321569383659 1.3773772670768955E-4 36-37 8.608607919230598E-4 0.0 0.0 0.15628066816571226 1.3773772670768955E-4 38-39 9.985985186307493E-4 0.0 0.0 0.24346864917167976 1.3773772670768955E-4 40-41 0.0010158157344692105 0.0 0.0 0.3418306032568086 1.3773772670768955E-4 42-43 0.0011535534611769 0.0 0.0 0.4404335983636758 1.3773772670768955E-4 44-45 0.0012740739720461284 0.0 0.0 0.5574245799860196 1.3773772670768955E-4 46-47 0.001325725619561512 0.0 0.0 0.703409353080332 1.3773772670768955E-4 48-49 0.0013773772670768955 0.0 0.0 0.8655955262786366 1.3773772670768955E-4 50-51 0.0013773772670768955 0.0 0.0 1.0410045212408792 1.7217215838461194E-4 52-53 0.0013773772670768955 0.0 0.0 1.2069612647078065 1.7217215838461194E-4 54-55 0.0014118116987538178 0.0 0.0 1.3866229119821492 2.0660659006153432E-4 56-57 0.0015151149937845851 0.0 0.0 1.5960014737936759 2.0660659006153432E-4 58-59 0.0015151149937845851 0.0 0.0 1.848629681791417 2.0660659006153432E-4 60-61 0.0015151149937845851 0.0 0.0 2.1430096381974266 2.0660659006153432E-4 62-63 0.0015495494254615075 0.0 0.0 2.4255441501065746 2.0660659006153432E-4 64-65 0.0015495494254615075 0.0 0.0 2.692118302933469 2.0660659006153432E-4 66-67 0.0016528527204922746 0.0 0.0 2.9935745350490865 2.0660659006153432E-4 68-69 0.0016528527204922746 0.0 0.0 3.336593126198749 2.0660659006153432E-4 70-71 0.0017389387996845805 0.0 0.0 3.726339241133995 2.0660659006153432E-4 72-73 0.0018078076630384254 0.0 0.0 4.129841911524171 2.0660659006153432E-4 74-75 0.0018422420947153478 0.0 0.0 4.526956994838279 2.0660659006153432E-4 76-77 0.001859459310553809 0.0 0.0 4.945145950338663 2.238238058999955E-4 78-79 0.0018766765263922701 0.0 0.0 5.412920487453815 2.410410217384567E-4 80-81 0.0021693691956461104 0.0 0.0 5.933379705034659 2.410410217384567E-4 82-83 0.0022726724906768775 0.0 0.0 6.459761644863932 2.410410217384567E-4 84-85 0.002324324138192261 0.0 0.0 7.02884227997259 2.410410217384567E-4 86-87 0.002341541354030722 0.0 0.0 7.608718109411964 2.410410217384567E-4 88-89 0.002341541354030722 0.0 0.0 8.193225369911882 2.410410217384567E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 22755 0.0 23.710018 1 TACCTGG 2405 0.0 23.641253 2 GAGTACT 13595 0.0 23.126041 12-13 TACATGG 23120 0.0 22.952738 2 CATGGGG 6355 0.0 22.367117 4 AGTACTT 14010 0.0 22.136839 12-13 ACATGGG 23120 0.0 21.74362 3 AGTTCGC 110 0.0029323115 21.521982 8 GTACTTT 14900 0.0 21.195906 14-15 ATGGGAT 5595 0.0 19.308079 5 CATGGGA 15805 0.0 18.976387 4 ACTTTTT 16995 0.0 18.890497 16-17 TACTTTT 16385 0.0 18.754723 14-15 ATGGGAG 4605 0.0 18.726046 5 ATGGGGA 3395 0.0 18.561632 5 GTATAGG 755 0.0 17.582375 1 TACACCG 385 2.6011548E-10 17.229458 5 ACCTGGG 3350 0.0 16.830866 3 TCCCGTA 170 0.001818704 16.72271 2 GTACCTG 3525 0.0 16.677425 1 >>END_MODULE