##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938713_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2904070 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00518410368896 33.0 31.0 34.0 30.0 34.0 2 32.20920742268609 34.0 31.0 34.0 30.0 34.0 3 32.29528454892616 34.0 31.0 34.0 30.0 34.0 4 35.91890760208948 37.0 35.0 37.0 35.0 37.0 5 35.832702379763575 37.0 35.0 37.0 35.0 37.0 6 35.87871745515776 37.0 35.0 37.0 35.0 37.0 7 35.81467457740344 37.0 35.0 37.0 33.0 37.0 8 35.79256698357822 37.0 35.0 37.0 33.0 37.0 9 37.42275737155096 39.0 37.0 39.0 34.0 39.0 10-11 37.35111326517611 39.0 37.0 39.0 34.0 39.0 12-13 37.276752109969806 39.0 37.0 39.0 33.0 39.0 14-15 38.43470078200595 40.0 38.0 41.0 33.5 41.0 16-17 38.382477867269046 40.0 38.0 41.0 33.0 41.0 18-19 38.454718378000535 40.0 38.0 41.0 33.5 41.0 20-21 38.5424814140155 40.0 38.0 41.0 34.0 41.0 22-23 38.49884369178429 40.0 38.0 41.0 34.0 41.0 24-25 38.41605402073641 40.0 38.0 41.0 34.0 41.0 26-27 38.21516595674347 40.0 38.0 41.0 33.5 41.0 28-29 38.04346951003247 40.0 37.0 41.0 33.0 41.0 30-31 37.816469472154594 40.0 37.0 41.0 33.0 41.0 32-33 37.583810135430625 40.0 36.0 41.0 32.5 41.0 34-35 37.533199957301306 40.0 36.0 41.0 32.0 41.0 36-37 37.41302568464259 40.0 36.0 41.0 32.0 41.0 38-39 37.28654474582224 40.0 36.0 41.0 31.5 41.0 40-41 37.106898249697835 40.0 36.0 41.0 31.0 41.0 42-43 36.92046679315581 40.0 36.0 41.0 31.0 41.0 44-45 36.63479513234874 39.5 35.0 41.0 30.5 41.0 46-47 36.26883425675001 39.0 35.0 41.0 30.0 41.0 48-49 35.938867348238844 39.0 35.0 41.0 28.5 41.0 50-51 35.68489774695514 39.0 35.0 41.0 28.0 41.0 52-53 35.43543750667167 39.0 35.0 40.5 27.0 41.0 54-55 35.200022554552746 38.5 35.0 40.0 27.0 41.0 56-57 34.96266653352019 38.0 34.0 40.0 26.0 41.0 58-59 34.681576718192055 38.0 34.0 40.0 26.0 41.0 60-61 34.30518048807363 37.5 34.0 40.0 25.5 41.0 62-63 33.91634722992214 37.0 33.0 40.0 24.5 41.0 64-65 33.5113123994945 36.0 33.0 39.0 23.0 41.0 66-67 33.11106120031542 36.0 32.5 39.0 22.5 40.5 68-69 32.68372680410596 35.0 32.0 38.5 22.0 40.0 70-71 32.19427562007803 35.0 31.5 37.5 20.5 40.0 72-73 31.82628535124842 35.0 31.0 37.0 20.0 39.0 74-75 31.409082942215584 35.0 31.0 37.0 18.0 39.0 76-77 29.365872034765005 32.5 28.5 35.0 13.0 37.0 78-79 30.412529312309964 34.0 30.0 36.0 13.0 37.0 80-81 30.482783472850173 34.0 31.0 35.0 12.5 37.0 82-83 30.19450237081062 34.0 30.5 35.0 8.5 37.0 84-85 29.833631420730217 34.0 30.0 35.0 4.5 36.0 86-87 29.31242101602234 34.0 29.5 35.0 2.0 36.0 88-89 28.78687893198167 34.0 29.0 35.0 2.0 35.5 90-91 28.365149772560578 34.0 29.0 35.0 2.0 35.0 92-93 28.124049179255323 34.0 29.0 35.0 2.0 35.0 94-95 27.768631265775273 33.5 27.5 35.0 2.0 35.0 96-97 27.251885285134314 33.0 27.0 35.0 2.0 35.0 98-99 26.533117659009598 33.0 25.5 35.0 2.0 35.0 100-101 23.388476000922843 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.029778653359805674 1101 2 0.040896492371473414 1101 3 0.11696491304323331 1101 4 0.021256299664301537 1101 5 0.037291880171174796 1101 6 0.15040156768050394 1101 7 0.12693300776493288 1101 8 0.08090615617737029 1101 9 0.1512182717467141 1101 10-11 0.1064853392427878 1101 12-13 0.17908982901023762 1101 14-15 0.18663904826078692 1101 16-17 0.07972099051202974 1101 18-19 0.18903520594964363 1101 20-21 0.1847749290174434 1101 22-23 0.11707564932060421 1101 24-25 0.12249157828656365 1101 26-27 0.14012001232801907 1101 28-29 0.1167987222735718 1101 30-31 0.12700557889554176 1101 32-33 0.27082062467182766 1101 34-35 0.27349638675399035 1101 36-37 0.18119851490617833 1101 38-39 0.32323242398530283 1101 40-41 0.3839979485709506 1101 42-43 0.32570752476433285 1101 44-45 0.36376714326384985 1101 46-47 0.48743192438242744 1101 48-49 0.27666403245640936 1101 50-51 0.3463040873582557 1101 52-53 0.36463211274018903 1101 54-55 0.35081980407559143 1101 56-57 0.2803178936631383 1101 58-59 0.35814469058350795 1101 60-61 0.19746392989684836 1101 62-63 0.12802710742396073 1101 64-65 0.1603054721024364 1101 66-67 0.029915167279511934 1101 68-69 0.27154986477054166 1101 70-71 0.24093473774431828 1101 72-73 0.3189055169189139 1101 74-75 0.27664666980812314 1101 76-77 0.15293534177180845 1101 78-79 0.2766336170070183 1101 80-81 0.29627249086224694 1101 82-83 0.3075184676895937 1101 84-85 0.46074349660086966 1101 86-87 0.49819687965565507 1101 88-89 0.5716303812084575 1101 90-91 0.6860604947366404 1101 92-93 0.6516221055017795 1101 94-95 0.678326306466623 1101 96-97 0.41161907392661234 1101 98-99 0.33827136659829904 1101 100-101 0.7584448877226144 1102 1 0.0864858472057577 1102 2 0.1608796730488109 1102 3 0.18234696591327548 1102 4 0.1535209758271492 1102 5 0.16400774444314692 1102 6 0.16304252729457147 1102 7 0.17019556521465518 1102 8 0.16136379821263347 1102 9 0.1985104259890278 1102 10-11 0.2020697455141729 1102 12-13 0.18297150703103426 1102 14-15 0.23936299623822777 1102 16-17 0.3085619566452138 1102 18-19 0.2936588899028365 1102 20-21 0.3293111916074949 1102 22-23 0.2673649677468859 1102 24-25 0.25231021644295737 1102 26-27 0.27986192047365677 1102 28-29 0.21684520358000725 1102 30-31 0.36094123520767596 1102 32-33 0.30737983455480844 1102 34-35 0.358069612739186 1102 36-37 0.3370886025883024 1102 38-39 0.30599815950471765 1102 40-41 0.38777421246481936 1102 42-43 0.30995609870742413 1102 44-45 0.13892239604261647 1102 46-47 0.2242444671641337 1102 48-49 0.1977808224430433 1102 50-51 0.23218700401133674 1102 52-53 0.11915494594857279 1102 54-55 0.06139400792838501 1102 56-57 0.07801677563451648 1102 58-59 0.07736226612377806 1102 60-61 0.11048736612358567 1102 62-63 0.050725174505686255 1102 64-65 0.05757213659606464 1102 66-67 0.02833649783135428 1102 68-69 0.1269061671272098 1102 70-71 0.0568541614543463 1102 72-73 0.05605477210976062 1102 74-75 0.20822116702120042 1102 76-77 0.02946541120040891 1102 78-79 0.08068459650439408 1102 80-81 0.11784822670761486 1102 82-83 0.16271850680429978 1102 84-85 0.20885947169784203 1102 86-87 0.2676047482302515 1102 88-89 0.3581954582970184 1102 90-91 0.41791387978830485 1102 92-93 0.3929331651842958 1102 94-95 0.5017842418621719 1102 96-97 0.5346028087289234 1102 98-99 0.6929269290351421 1102 100-101 0.9621255813566947 1103 1 0.06657515957348181 1103 2 0.062609218359718 1103 3 0.005845295818353691 1103 4 0.13143554888559095 1103 5 -0.035770513457805464 1103 6 -0.003827647823172242 1103 7 0.06668792892995867 1103 8 0.0774200767322597 1103 9 0.06079741178717768 1103 10-11 -0.0027505234462736894 1103 12-13 0.0037998254779196827 1103 14-15 0.1586188176965777 1103 16-17 0.11311259643348137 1103 18-19 0.07197891360193864 1103 20-21 0.09946976861797197 1103 22-23 0.01285144328996779 1103 24-25 0.07285521465019684 1103 26-27 0.10899442226989464 1103 28-29 0.023023763216386328 1103 30-31 0.1187061665452589 1103 32-33 0.1527736769180592 1103 34-35 0.0858382511770941 1103 36-37 0.04244600214165217 1103 38-39 0.22518900855227741 1103 40-41 0.27945114532121806 1103 42-43 0.3058686628138574 1103 44-45 0.36172970066405696 1103 46-47 0.40815020574783745 1103 48-49 0.40266891348142764 1103 50-51 0.4845031817996599 1103 52-53 0.37617554422921984 1103 54-55 0.42845937891261343 1103 56-57 0.37099308071557147 1103 58-59 0.2096171545377814 1103 60-61 0.22525796348600835 1103 62-63 0.28628682933398863 1103 64-65 0.28863391370675373 1103 66-67 0.15659292329247876 1103 68-69 0.13101500718729397 1103 70-71 0.2171644028160884 1103 72-73 0.14394832222381737 1103 74-75 0.1334478643747694 1103 76-77 0.02402856292719946 1103 78-79 0.10452674824879082 1103 80-81 0.2304794206332872 1103 82-83 0.24097847218527235 1103 84-85 0.12072711947746484 1103 86-87 0.1617900847550402 1103 88-89 0.30676637555674446 1103 90-91 0.40027551673336603 1103 92-93 0.2927504571924757 1103 94-95 0.27412971676173115 1103 96-97 0.08463606117814848 1103 98-99 0.3603246725293836 1103 100-101 0.9103147698715475 1104 1 5.462515203973339E-4 1104 2 0.09272953092824565 1104 3 0.02008765800255219 1104 4 0.02752584335019037 1104 5 0.03365127175235472 1104 6 0.06181154291927271 1104 7 -0.01163225576531346 1104 8 -0.04053706985730088 1104 9 -0.025510415908044592 1104 10-11 0.08389672940903381 1104 12-13 0.033747770290752044 1104 14-15 0.05725595664422656 1104 16-17 0.12781296079855053 1104 18-19 0.036842068347944235 1104 20-21 0.020630606413980956 1104 22-23 0.0241991017436618 1104 24-25 0.06082833102798446 1104 26-27 0.20727459315574492 1104 28-29 0.13792995679780518 1104 30-31 0.08150009605153485 1104 32-33 0.05444283903334224 1104 34-35 0.1412310878505565 1104 36-37 0.04595833096560398 1104 38-39 0.08959606034893852 1104 40-41 0.11341995069334132 1104 42-43 0.11591127432217263 1104 44-45 0.21868189388288073 1104 46-47 0.20912842243689767 1104 48-49 0.2120181180595253 1104 50-51 0.2997975433186326 1104 52-53 0.14994445798249245 1104 54-55 0.07196518787466744 1104 56-57 0.11524962341198375 1104 58-59 0.01852247622198888 1104 60-61 0.15144270598457155 1104 62-63 -2.3536162449744324E-4 1104 64-65 0.13702001048233825 1104 66-67 0.006881562751743786 1104 68-69 0.05725485238993144 1104 70-71 0.034955254192851726 1104 72-73 -0.02279027367181996 1104 74-75 -0.050002781907476646 1104 76-77 -0.06218386586879987 1104 78-79 0.024153956589618275 1104 80-81 0.09856679577912075 1104 82-83 0.09770806797257947 1104 84-85 0.08449659175474267 1104 86-87 0.310588814568991 1104 88-89 0.5457690441019842 1104 90-91 0.5434676401204435 1104 92-93 0.5712775706591913 1104 94-95 0.5570875374535369 1104 96-97 0.6253558974144084 1104 98-99 0.6273460040231278 1104 100-101 1.0265241236617726 1105 1 -0.05055773905537464 1105 2 -0.05256854136369782 1105 3 -0.03875177547401876 1105 4 -0.17413665911803378 1105 5 -0.05503402396338686 1105 6 -0.1700907419795854 1105 7 -0.10347264567413816 1105 8 -0.09303558270281798 1105 9 -0.03207044375867696 1105 10-11 -0.06671652818278773 1105 12-13 -0.09239733243894221 1105 14-15 0.04066539686820647 1105 16-17 -0.032569355793086174 1105 18-19 -0.16650311784403726 1105 20-21 -0.10115107744180563 1105 22-23 -0.1490337101907997 1105 24-25 -0.10688449692208479 1105 26-27 -0.19403552119931078 1105 28-29 -0.09886136790005651 1105 30-31 -0.15334987149297064 1105 32-33 -0.058290749356885385 1105 34-35 -0.06199384443586098 1105 36-37 -0.05702218665746983 1105 38-39 -0.08031088813697806 1105 40-41 -0.09951102082757046 1105 42-43 -0.13872204707079305 1105 44-45 0.06615757590541449 1105 46-47 0.18335377390118168 1105 48-49 0.12300285508295872 1105 50-51 0.1060257002511591 1105 52-53 0.0846404905741096 1105 54-55 0.0030029013160159934 1105 56-57 -0.06465227229472248 1105 58-59 0.10011098738905844 1105 60-61 -0.02464453427351998 1105 62-63 -0.18979663829918536 1105 64-65 -0.06386784033960424 1105 66-67 -0.11013164626572092 1105 68-69 -0.20188690176927793 1105 70-71 -0.23998797513900172 1105 72-73 -0.4166883180185721 1105 74-75 -0.4065292966805423 1105 76-77 -0.35209960850478694 1105 78-79 -0.31329187782878165 1105 80-81 -0.2215955102889069 1105 82-83 -0.25846707894807963 1105 84-85 -0.14614903049918482 1105 86-87 0.0339455446382253 1105 88-89 0.148665203579192 1105 90-91 0.15567038185971782 1105 92-93 0.16570562802920108 1105 94-95 0.2636600448351807 1105 96-97 0.1566216640203031 1105 98-99 0.25265393302940353 1105 100-101 0.7600961523918173 1106 1 -0.020648648045412443 1106 2 -0.07069806519154298 1106 3 -0.12452532052271437 1106 4 -0.01940915615572436 1106 5 -0.03082996923774317 1106 6 -0.13722791324865824 1106 7 0.012450772159667167 1106 8 -0.04120708984755339 1106 9 -0.020535480284642915 1106 10-11 -0.05514618100703217 1106 12-13 0.020836061046686893 1106 14-15 0.003182590943126229 1106 16-17 -0.06523251934999763 1106 18-19 -0.0555797129554918 1106 20-21 -4.627991732775172E-5 1106 22-23 -0.09017662101684465 1106 24-25 -0.06594604717945884 1106 26-27 -0.05569511050919118 1106 28-29 -0.059474992795095716 1106 30-31 -0.09405282852248575 1106 32-33 -0.05125820781709933 1106 34-35 -0.10931615506801506 1106 36-37 -0.016828952193840507 1106 38-39 -0.043820120385845485 1106 40-41 -0.12169290316406745 1106 42-43 -0.13925841220790858 1106 44-45 -0.07467800219707499 1106 46-47 -0.020261922604795757 1106 48-49 0.05884973809322247 1106 50-51 -0.028677463162729566 1106 52-53 -0.023910665196915204 1106 54-55 -0.05534716530274153 1106 56-57 -0.18099530137876485 1106 58-59 -0.1078607263619702 1106 60-61 -0.13130827161352698 1106 62-63 -0.07561633010590185 1106 64-65 -0.03878677529265673 1106 66-67 -0.05212173167852541 1106 68-69 -0.12998752808545788 1106 70-71 -0.2169123769356034 1106 72-73 -0.2462426843365435 1106 74-75 -0.11177387634743852 1106 76-77 -0.1995171962264699 1106 78-79 -0.1727510817152158 1106 80-81 -0.226918599855658 1106 82-83 -0.13366580404988326 1106 84-85 -0.1554452042881458 1106 86-87 0.06808274142795057 1106 88-89 0.15459918597537126 1106 90-91 0.20918798414173523 1106 92-93 0.19340631988536217 1106 94-95 0.2223177030826271 1106 96-97 0.10097592317620396 1106 98-99 0.3211444507521364 1106 100-101 0.6012081672051366 1107 1 -0.07192052930389536 1107 2 -0.08437742489279998 1107 3 -0.06571407107977478 1107 4 -0.11122746321422028 1107 5 -0.05044394170587907 1107 6 -0.07965123036540689 1107 7 -0.17499277434135507 1107 8 -0.07436519321041146 1107 9 -0.13456596399687726 1107 10-11 -0.1016592760454742 1107 12-13 -0.153944273642189 1107 14-15 -0.194308999213618 1107 16-17 -0.1287209347690279 1107 18-19 -0.15348663665831452 1107 20-21 -0.11881834439876826 1107 22-23 -0.2286011740282916 1107 24-25 -0.23785784080967431 1107 26-27 -0.20725937576580833 1107 28-29 -0.11669947708132611 1107 30-31 -0.15850082513790653 1107 32-33 -0.059364450328956764 1107 34-35 -0.22340042845284813 1107 36-37 -0.1696547090314411 1107 38-39 -0.15294300582085185 1107 40-41 -0.14074748718516616 1107 42-43 -0.19458890839258203 1107 44-45 -0.22864897799002648 1107 46-47 -0.4282526652270704 1107 48-49 -0.532241050060108 1107 50-51 -0.38841065914223094 1107 52-53 -0.3658172030146787 1107 54-55 -0.43459146326541287 1107 56-57 -0.3678076191292732 1107 58-59 -0.36770154998267657 1107 60-61 -0.340479827344879 1107 62-63 -0.3029421720048333 1107 64-65 -0.21394098140960693 1107 66-67 -0.199000499898645 1107 68-69 -0.15339448746694728 1107 70-71 -0.22345056899165883 1107 72-73 -0.02616528757800296 1107 74-75 0.024963395452441972 1107 76-77 -0.047275036207086174 1107 78-79 -0.15921769668101504 1107 80-81 -0.07058643250856278 1107 82-83 -0.04976979210434962 1107 84-85 -0.15738978245214952 1107 86-87 -0.10647452610014696 1107 88-89 0.028988286823338427 1107 90-91 0.13297465876758352 1107 92-93 0.14647739626681755 1107 94-95 0.0774050651175493 1107 96-97 0.13507712672466 1107 98-99 0.047149517727220314 1107 100-101 0.6130604891380784 1108 1 -0.04349475814145265 1108 2 -0.04975918517028077 1108 3 -0.09407007591580907 1108 4 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-0.7300623199741594 2316 52-53 -0.9394298077543226 2316 54-55 -0.853444610290822 2316 56-57 -0.7825236282381809 2316 58-59 -0.7947406339395684 2316 60-61 -0.7397478360628682 2316 62-63 -0.6108428701951283 2316 64-65 -0.7242716354324656 2316 66-67 -0.6691351364612927 2316 68-69 -0.7014809356131266 2316 70-71 -0.8396089050138613 2316 72-73 -0.9621129206810011 2316 74-75 -0.9846425767015212 2316 76-77 -0.8552729071890788 2316 78-79 -0.7932043878954644 2316 80-81 -0.7801615920536662 2316 82-83 -0.9959224360725294 2316 84-85 -1.0525091944361122 2316 86-87 -1.2059912264937687 2316 88-89 -1.5198584816285887 2316 90-91 -1.9471527632831815 2316 92-93 -2.1047922609055547 2316 94-95 -2.0846805143795883 2316 96-97 -2.102959312516955 2316 98-99 -2.5082323102641126 2316 100-101 -3.278676502024801 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 15.0 4 39.0 5 109.0 6 296.0 7 694.0 8 1344.0 9 2340.0 10 3815.0 11 5706.0 12 8012.0 13 11053.0 14 13246.0 15 17839.0 16 23196.0 17 31742.0 18 29359.0 19 19004.0 20 16745.0 21 17553.0 22 18801.0 23 20373.0 24 24078.0 25 28292.0 26 33637.0 27 41380.0 28 50353.0 29 61720.0 30 74763.0 31 89605.0 32 110646.0 33 138716.0 34 185728.0 35 263074.0 36 392072.0 37 607337.0 38 509499.0 39 51881.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56273151764961 19.287641192062686 14.655421540146317 21.494205750141383 2 15.46835992245366 21.908390637966715 38.991105586297856 23.63214385328177 3 20.455567531085684 26.500600880832764 29.831684497963202 23.21214709011835 4 12.943902867355126 17.022730168349938 37.08891314603298 32.944453818261955 5 13.22481896097546 38.97526574772647 33.00633249198538 14.793582799312688 6 30.295447423787998 36.28108137889238 17.592069061696172 15.831402135623451 7 27.93469165688155 34.30674880426436 20.180746331872164 17.577813206981926 8 26.24072422496703 33.494612733164146 19.641124353063113 20.62353868880571 9 27.05103527118837 17.813034809766982 20.549745701722067 34.58618421732258 10-11 24.91238158859807 26.654230097759353 26.465477760522298 21.967910553120277 12-13 27.6855068920515 24.217064326961815 26.807824880254266 21.28960390073242 14-15 24.101173390118124 25.106695306990478 27.012214606840622 23.779916696050776 16-17 21.512900808676573 28.92254969345772 27.545847044287818 22.018702453577887 18-19 21.2500434735149 27.828851744811672 30.059723139996425 20.861381641677 20-21 22.817786761338397 26.274349447499546 29.1843343996529 21.723529391509157 22-23 22.111261092191302 26.34580089322916 28.881604093565237 22.6613339210143 24-25 22.42246226847149 26.478339020753634 29.48799443539584 21.611204275379038 26-27 22.610334881965574 25.911325079164815 30.086124016379785 21.392216022489833 28-29 21.525169847834245 26.513307186121548 30.48402758886666 21.477495377177547 30-31 21.56785132589779 26.963330773707245 29.874555365400973 21.59426253499399 32-33 21.466631313983477 27.02345329141515 29.42031355993485 22.08960183466652 34-35 22.255428980735985 26.277988608266238 29.389553904113836 22.07702850688394 36-37 22.71926848798464 25.931016075010977 29.79769766580853 21.552017771195853 38-39 22.802179873484135 25.83155933205602 30.078400438881285 21.287860355578562 40-41 21.88489680590315 26.363674713679753 30.285455026004072 21.465973454413028 42-43 21.57952362119381 27.02701353199008 29.238985037898974 22.154477808917136 44-45 21.833853848231872 27.048064998275663 28.568769691484086 22.549311462008383 46-47 22.143484720423235 26.927640220542294 28.051150412522492 22.877724646511986 48-49 22.55941188735325 26.790405670387706 28.047727507898827 22.602454934360214 50-51 22.55611278271607 27.304582340184197 28.138655441929274 22.00064943517046 52-53 22.23538000117077 27.547596993185426 28.118554304820474 22.098468700823325 54-55 21.36012561680676 26.992427868474245 28.581439152637504 23.066007362081493 56-57 22.26402944832597 27.734593174406953 27.418209616159388 22.583167761107685 58-59 22.51558551055858 26.79511215248861 27.74798519107477 22.94131714587804 60-61 22.15715611446753 26.66464650290714 28.547892515809792 22.63030486681554 62-63 22.981574135609677 26.563891366254943 28.132018167606155 22.322516330529226 64-65 22.387958967931212 26.951743587447965 28.334509843082294 22.32578760153853 66-67 21.851827492377843 27.062045416823633 28.16378871597233 22.92233837482619 68-69 21.580741056520168 27.569030486118834 28.05235666276291 22.79787179459808 70-71 21.89126414853943 27.405553011181087 27.432911732397613 23.27027110788186 72-73 21.806344199692155 26.95349974346348 27.43038218775718 23.809773869087177 74-75 21.66855482133695 27.65393051820376 27.354058269945288 23.323456390514004 76-77 22.13139631136501 26.647994789660462 27.94840812511019 23.272200773864334 78-79 21.64689728770057 26.66906991400152 28.352439608937807 23.331593189360103 80-81 21.563154738548786 27.615385926542046 27.778398554166834 23.043060780742334 82-83 21.48448556680796 27.207350373785754 27.63383802732028 23.674326032086004 84-85 21.325467361323934 26.706432696181565 27.606152744252032 24.36194719824247 86-87 21.7377884654845 26.634041418810313 27.828717966049553 23.799452149655636 88-89 21.94503041236864 26.128104372844653 27.83863168870006 24.088233526086647 90-91 21.27516896715685 26.292802049187546 28.373067189414837 24.05896179424077 92-93 21.21217808110686 26.969838881294184 27.969677039465303 23.848305998133654 94-95 20.821182279071195 26.73801827711394 27.72558090045381 24.715218543361058 96-97 20.649746846623334 26.374523750206393 27.649940884679243 25.32578851849103 98-99 21.02013901204002 26.408224333821728 27.3592016707615 25.212434983376745 100-101 21.323213676343837 25.89102257501676 28.064331514839097 24.721432233800304 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3412.0 1 2260.0 2 1407.0 3 1724.5 4 1502.0 5 1487.0 6 2533.5 7 4918.0 8 7247.0 9 7724.0 10 6479.0 11 5175.5 12 4954.0 13 5186.0 14 5336.5 15 5342.0 16 5396.0 17 5530.0 18 5723.5 19 5948.5 20 6282.0 21 6988.5 22 7996.0 23 9247.5 24 10456.0 25 12124.0 26 15056.5 27 18391.0 28 21427.0 29 25113.5 30 29367.0 31 33843.0 32 39313.0 33 44716.0 34 50394.5 35 54941.5 36 61055.0 37 68150.0 38 70716.0 39 70817.5 40 71962.0 41 76265.0 42 80931.5 43 86031.0 44 92150.5 45 96651.5 46 99122.0 47 113262.0 48 177058.0 49 179569.0 50 124078.5 51 107797.0 52 93886.0 53 89810.0 54 89154.0 55 84320.0 56 77540.0 57 69413.0 58 63038.5 59 56944.0 60 49541.0 61 41427.0 62 33510.0 63 25944.0 64 19159.5 65 14678.5 66 10853.0 67 8509.0 68 6618.5 69 4520.0 70 3389.0 71 2800.5 72 2420.0 73 2248.0 74 1852.0 75 1413.0 76 942.5 77 495.0 78 303.0 79 211.5 80 119.0 81 58.5 82 40.0 83 29.0 84 14.5 85 6.5 86 1.0 87 0.5 88 1.0 89 1.5 90 2.0 91 1.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3857345036448846 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.0660659006153432E-4 16-17 0.0022726724906768775 18-19 1.0330329503076716E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.886886335384477E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007231230652153702 36-37 0.01704504368007658 38-39 0.011139538647484392 40-41 0.006800800256192172 42-43 0.0034262259518537774 44-45 0.0018766765263922701 46-47 0.0016012010729768908 48-49 1.7217215838461194E-5 50-51 2.754754534153791E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.615615326076851E-4 60-61 5.165164751538358E-4 62-63 0.0 64-65 0.0 66-67 0.001239639540369206 68-69 9.297296552769045E-4 70-71 0.0020660659006153433 72-73 0.0 74-75 0.0 76-77 0.10039358555406722 78-79 0.036276673771637735 80-81 1.7217215838461194E-5 82-83 0.0 84-85 0.0 86-87 0.1505817697231816 88-89 0.32795352729100885 90-91 0.2786778555613329 92-93 0.0 94-95 3.443443167692239E-5 96-97 1.7217215838461194E-5 98-99 1.7217215838461194E-4 100-101 6.542542018615254E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2904070.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.182651350912984 #Duplication Level Percentage of deduplicated Percentage of total 1 65.12213725046182 23.562915873597632 2 15.742085373725953 11.391807732276657 3 6.829326375442147 7.413094056126522 4 3.5889596525112846 5.1943230327723855 5 2.2439015898556893 4.059515444575387 6 1.4464179504080332 3.1401141844389606 7 0.9735868175654996 2.4658866665872177 8 0.7211883046430632 2.0875603988204783 9 0.5104040055449318 1.6620993162667554 >10 2.502252252357876 15.94696119042025 >50 0.18526383483245948 4.638093343170289 >100 0.12120733270752759 8.278974643393223 >500 0.006968748028015726 1.7188534230931394 >1k 0.005059501992936902 3.6626364824826787 >5k 6.682361122746852E-4 1.6273894063653724 >10k+ 5.727738105211588E-4 3.1497748056130392 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19751 0.680114460050894 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17797 0.6128295805541878 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16868 0.5808399935263269 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14167 0.4878325935669595 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12204 0.4202378041851609 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10403 0.3582213927350236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9857 0.339420193039424 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6982 0.24042120196827213 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6969 0.2399735543564721 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6431 0.22144783011428787 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6240 0.2148708536639957 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5381 0.18529167685351935 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5255 0.18095293846222715 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4995 0.17199998622622734 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4248 0.1462774657635663 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4017 0.13832311204619724 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3846 0.1324348242294435 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3615 0.12448047051207443 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3557 0.12248327347481294 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3308 0.11390909998725926 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3137 0.10802081217050553 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2942 0.10130609799350566 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2920 0.10054854049661337 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2908 0.1001353273164903 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.443443167692239E-5 2 3.443443167692239E-5 0.0 0.0 0.0 3.443443167692239E-5 3 3.443443167692239E-5 0.0 0.0 0.0 3.443443167692239E-5 4 3.443443167692239E-5 0.0 0.0 3.443443167692239E-5 3.443443167692239E-5 5 1.0330329503076716E-4 0.0 0.0 3.443443167692239E-5 3.443443167692239E-5 6 1.3773772670768955E-4 0.0 0.0 3.443443167692239E-5 3.443443167692239E-5 7 2.0660659006153432E-4 0.0 0.0 3.443443167692239E-5 3.443443167692239E-5 8 2.0660659006153432E-4 0.0 0.0 3.443443167692239E-5 3.443443167692239E-5 9 2.0660659006153432E-4 0.0 0.0 6.886886335384477E-5 3.443443167692239E-5 10-11 2.410410217384567E-4 0.0 0.0 1.2052051086922835E-4 3.443443167692239E-5 12-13 2.582582375769179E-4 0.0 0.0 1.7217215838461194E-4 3.443443167692239E-5 14-15 3.9599596428460746E-4 0.0 0.0 3.271271009307627E-4 5.165164751538358E-5 16-17 4.1321318012306864E-4 0.0 0.0 3.4434431676922387E-4 1.0330329503076716E-4 18-19 4.47647611799991E-4 0.0 0.0 4.304303959615298E-4 1.0330329503076716E-4 20-21 4.820820434769134E-4 0.0 0.0 8.264263602461373E-4 1.0330329503076716E-4 22-23 4.820820434769134E-4 0.0 0.0 0.0019283281739076537 1.0330329503076716E-4 24-25 7.575574968922926E-4 0.0 0.0 0.0038566563478153078 1.0330329503076716E-4 26-27 7.575574968922926E-4 0.0 0.0 0.005957156680107573 1.0330329503076716E-4 28-29 8.780780077615208E-4 0.0 0.0 0.01210370273443822 1.0330329503076716E-4 30-31 9.641640869538268E-4 0.0 0.0 0.027857455226630214 1.0330329503076716E-4 32-33 9.641640869538268E-4 0.0 0.0 0.0553705661364912 1.0330329503076716E-4 34-35 0.0011191190294999776 0.0 0.0 0.09545224460842885 1.2052051086922835E-4 36-37 0.0012740739720461284 0.0 0.0 0.1585189062247122 1.3773772670768955E-4 38-39 0.0013085084037230508 0.0 0.0 0.24680878904434123 1.3773772670768955E-4 40-41 0.0013429428353999731 0.0 0.0 0.34641038266983926 1.3773772670768955E-4 42-43 0.0014118116987538178 0.0 0.0 0.44618414845372184 1.3773772670768955E-4 44-45 0.0014806805621076628 0.0 0.0 0.5640015564363118 1.3773772670768955E-4 46-47 0.0015839838571384299 0.0 0.0 0.7108644075383858 1.5495494254615073E-4 48-49 0.0016528527204922746 0.0 0.0 0.875168298284821 1.7217215838461194E-4 50-51 0.0016528527204922746 0.0 0.0 1.0523334492625867 1.7217215838461194E-4 52-53 0.0016528527204922746 0.0 0.0 1.2193748773273372 1.7217215838461194E-4 54-55 0.001687287152169197 0.0 0.0 1.4005860740271412 1.7217215838461194E-4 56-57 0.0017905904471999642 0.0 0.0 1.6113764475374217 1.7217215838461194E-4 58-59 0.0017905904471999642 0.0 0.0 1.8659846353565857 1.7217215838461194E-4 60-61 0.0017905904471999642 0.0 0.0 2.1623101371523417 1.7217215838461194E-4 62-63 0.0017905904471999642 0.0 0.0 2.4474961003006124 2.410410217384567E-4 64-65 0.0017905904471999642 0.0 0.0 2.716222405107315 2.754754534153791E-4 66-67 0.001859459310553809 0.0 0.0 3.0196930514760316 2.754754534153791E-4 68-69 0.0018938937422307313 0.0 0.0 3.3650531839797253 2.754754534153791E-4 70-71 0.0019627626055845762 0.0 0.0 3.7588109102053324 2.926926692538403E-4 72-73 0.00201441425309996 0.0 0.0 4.165705372115687 3.443443167692239E-4 74-75 0.002048848684776882 0.0 0.0 4.56598842314407 3.787787484461463E-4 76-77 0.0020660659006153433 0.0 0.0 4.987259260279539 3.787787484461463E-4 78-79 0.0020832831164538045 0.0 0.0 5.456101264776676 4.1321318012306864E-4 80-81 0.0024448446490614897 0.0 0.0 5.978867589279872 4.1321318012306864E-4 82-83 0.002479279080738412 0.0 0.0 6.508761841140193 4.1321318012306864E-4 84-85 0.002565365159930718 0.0 0.0 7.08020123481872 4.1321318012306864E-4 86-87 0.0026858856707999462 0.0 0.0 7.662022609647838 4.1321318012306864E-4 88-89 0.002754754534153791 0.0 0.0 8.249956096099613 4.1321318012306864E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2195 0.0 25.725704 2 GAGTACT 14220 0.0 21.858242 12-13 GTACATG 22900 0.0 21.507404 1 AGTACTT 14555 0.0 21.17583 12-13 TACATGG 23190 0.0 20.912453 2 GTACTTT 15365 0.0 20.167768 14-15 ACATGGG 23175 0.0 20.025066 3 ATGGGAG 5260 0.0 18.223024 5 TACTTTT 17020 0.0 18.053335 14-15 CATGGGA 16325 0.0 18.050665 4 ACTTTTT 17335 0.0 17.985037 16-17 GGAACGC 475 0.0 17.981823 8 TAGGTCG 380 2.1100277E-10 17.482327 5 ATACCGT 300 7.781091E-8 17.399078 6 ACCTGGG 3110 0.0 17.241413 3 CATGGGG 6320 0.0 16.893524 4 ATGGGAT 5300 0.0 16.83204 5 TGCACCG 625 0.0 16.703115 5 GTACCTG 3510 0.0 16.01319 1 CGTATAG 240 6.0077495E-5 15.877484 1 >>END_MODULE