FastQCFastQC Report
Thu 26 May 2016
SRR938712_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938712_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916507
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA193750.6643220811745009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184850.6338061249295818No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA158950.5450012634977389No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132530.45441344731900185No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT114840.3937586983333145No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT112040.38415817277311526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93430.32034896538907676No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA69070.23682439301534333No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT65590.22489231124766715No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG64440.22094923824972817No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT64210.2201606236501404No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC51190.17551817979521392No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG50710.17387237541346548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48950.16783775934705455No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47520.16293463379309564No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC45380.1555970892578005No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG35140.12048659578050044No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33400.11452055489666235No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT33080.11342335197549673No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT31030.10639439576177942No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2151.6916601E-924.2688452
TACCTGG22900.024.0279882
GAGTACT134000.022.81063512-13
GTACATG232300.022.3766611
TACATGG236500.021.781792
GTACTTT144550.021.16257914-15
AGTACTT138600.020.94061912-13
ACATGGG240800.020.7230723
CATGGGG70600.020.4250814
TATCACG1708.043951E-519.5318782
ATGGGAT54850.019.2851545
GTATAGG8000.019.0058441
ACTTTTT165850.018.87412516-17
ATGGGGA34650.018.2072075
CATGGGA163100.018.1478634
TACTTTT159750.018.07937614-15
GCTTATA25050.018.0195141
GACCGGT2956.4501364E-817.6871597
GTGTAAG9800.016.4846611
GTACCTG36100.016.1891881
Produced by FastQC (version 0.11.5)