##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938712_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2916507 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52209783827023 33.0 31.0 34.0 30.0 34.0 2 31.56048073945991 33.0 31.0 34.0 28.0 34.0 3 31.56894188836166 33.0 31.0 34.0 28.0 34.0 4 35.116040866694306 37.0 35.0 37.0 32.0 37.0 5 35.15514209292143 37.0 35.0 37.0 33.0 37.0 6 35.23131849160657 37.0 35.0 37.0 32.0 37.0 7 35.14645978905588 37.0 35.0 37.0 32.0 37.0 8 35.12013857672894 37.0 35.0 37.0 32.0 37.0 9 36.66082406111146 39.0 37.0 39.0 32.0 39.0 10-11 36.542492097567404 39.0 37.0 39.0 32.0 39.0 12-13 36.427589407465845 39.0 37.0 39.0 32.0 39.0 14-15 37.51368126323715 40.0 37.0 41.0 32.0 41.0 16-17 37.41823798125634 40.0 37.0 41.0 32.0 41.0 18-19 37.40669403502203 40.0 37.0 41.0 32.0 41.0 20-21 37.30910692139604 40.0 37.0 41.0 31.5 41.0 22-23 37.31865207249631 40.0 37.0 41.0 31.5 41.0 24-25 37.241045709816575 40.0 37.0 41.0 31.0 41.0 26-27 37.07115823826241 40.0 36.5 41.0 31.0 41.0 28-29 36.89005923867147 40.0 36.0 41.0 30.0 41.0 30-31 36.658719660196255 39.0 36.0 41.0 30.0 41.0 32-33 36.46285659523532 39.0 35.0 41.0 30.0 41.0 34-35 36.16084960536697 39.0 35.0 41.0 29.5 41.0 36-37 35.9172599277149 39.0 35.0 41.0 28.5 41.0 38-39 35.70853112987557 39.0 35.0 40.0 27.0 41.0 40-41 35.5752117173043 38.5 35.0 40.5 27.0 41.0 42-43 35.55818947117219 39.0 35.0 41.0 27.0 41.0 44-45 35.34491653885967 39.0 35.0 41.0 25.5 41.0 46-47 34.959707108537714 39.0 35.0 41.0 24.0 41.0 48-49 34.621551225489945 39.0 34.0 40.5 23.0 41.0 50-51 33.45450619525343 37.5 32.5 39.5 21.0 40.5 52-53 33.495531126789686 37.5 33.0 39.5 20.5 40.5 54-55 33.98287831985317 38.0 34.0 40.0 21.0 41.0 56-57 33.887929293500754 38.0 34.0 40.0 20.0 41.0 58-59 33.687051325438276 38.0 33.0 40.0 19.0 41.0 60-61 33.345460682933385 37.5 33.0 40.0 17.5 41.0 62-63 32.92753146143657 37.0 32.5 40.0 13.5 41.0 64-65 32.48859474707244 36.0 32.0 39.0 10.5 41.0 66-67 32.07821325304551 36.0 31.5 39.0 8.0 41.0 68-69 31.668625859632773 35.0 31.0 39.0 7.0 40.0 70-71 31.21624652366684 35.0 31.0 38.0 2.0 40.0 72-73 30.767744257085617 35.0 30.5 37.0 2.0 39.5 74-75 30.305251967507708 35.0 30.0 37.0 2.0 39.0 76-77 29.827151280624392 34.5 29.5 36.0 2.0 39.0 78-79 29.395546281905034 34.0 29.0 36.0 2.0 37.5 80-81 28.934070276532854 34.0 29.0 35.0 2.0 37.0 82-83 28.5282632614974 34.0 28.5 35.0 2.0 37.0 84-85 28.174204965048943 34.0 27.5 35.0 2.0 36.0 86-87 27.733711594040408 34.0 27.0 35.0 2.0 36.0 88-89 27.244638192193605 33.5 26.0 35.0 2.0 35.5 90-91 26.758188991145914 33.0 25.0 35.0 2.0 35.0 92-93 26.308930511738872 33.0 24.5 35.0 2.0 35.0 94-95 25.799076429441108 33.0 23.0 35.0 2.0 35.0 96-97 25.15585373187858 32.5 19.0 35.0 2.0 35.0 98-99 24.3697889290168 32.0 2.0 35.0 2.0 35.0 100-101 21.97018985382171 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18391227959962464 1101 2 0.22700888275202047 1101 3 0.29510093861039266 1101 4 0.06614046658405215 1101 5 0.11055194822179004 1101 6 0.22477370132150298 1101 7 0.2411027651994999 1101 8 0.24959178476001398 1101 9 0.4018209209128827 1101 10-11 0.26091310259901235 1101 12-13 0.3250326727855182 1101 14-15 0.23451153702202276 1101 16-17 0.44347085088707416 1101 18-19 0.45648054841883123 1101 20-21 0.4439526879143898 1101 22-23 0.35641035552871614 1101 24-25 0.4485782975388801 1101 26-27 0.4028099630587789 1101 28-29 0.44058712468638106 1101 30-31 0.49528544265405827 1101 32-33 0.431372680643328 1101 34-35 0.31925105106518714 1101 36-37 0.45641881610794854 1101 38-39 0.451142163495156 1101 40-41 0.5328342524493479 1101 42-43 0.7273976749220168 1101 44-45 0.7429801731088332 1101 46-47 0.5404167283534633 1101 48-49 0.5457380882757263 1101 50-51 0.603654171830712 1101 52-53 0.6106122433522785 1101 54-55 0.6928208980365653 1101 56-57 0.5457388201731419 1101 58-59 0.6131069956679127 1101 60-61 0.48296969781246446 1101 62-63 0.3797007786212845 1101 64-65 0.35275554248794805 1101 66-67 0.39161904470751807 1101 68-69 0.4827948286199977 1101 70-71 0.4078828413792479 1101 72-73 0.4295963666563303 1101 74-75 0.3760396858376076 1101 76-77 0.3664904806860072 1101 78-79 0.31653158757028876 1101 80-81 0.17328688956002836 1101 82-83 0.02048015088689681 1101 84-85 0.04012934182069827 1101 86-87 0.0339097554490273 1101 88-89 0.05839141469388309 1101 90-91 0.05106019497986125 1101 92-93 -0.013051732936130378 1101 94-95 -0.08314385607828001 1101 96-97 -0.2816016697241004 1101 98-99 -0.2178999285477694 1101 100-101 0.09639456960477943 1102 1 0.2189932187505299 1102 2 0.18543464472817917 1102 3 0.19706622306615174 1102 4 0.30011250424504254 1102 5 0.2294663729034454 1102 6 0.3443894856064915 1102 7 0.31654234121060654 1102 8 0.25668676805866397 1102 9 0.4407554017420665 1102 10-11 0.4178342901748522 1102 12-13 0.42786003247120874 1102 14-15 0.4082798279621187 1102 16-17 0.6161601550017295 1102 18-19 0.5175977824144908 1102 20-21 0.4939194426698492 1102 22-23 0.5263200180864587 1102 24-25 0.4722057957767021 1102 26-27 0.5240299969216906 1102 28-29 0.5890658973857938 1102 30-31 0.6377412549401384 1102 32-33 0.6510648260455554 1102 34-35 0.5721935882647315 1102 36-37 0.6163377074002412 1102 38-39 0.5066206649036928 1102 40-41 0.5267079834983406 1102 42-43 0.6921644374286444 1102 44-45 0.6413885191791096 1102 46-47 0.7270925914581454 1102 48-49 0.6040299003777179 1102 50-51 0.6952727974032697 1102 52-53 0.5128373130522377 1102 54-55 0.5827636968380361 1102 56-57 0.5301568527813387 1102 58-59 0.5099096388162394 1102 60-61 0.33887645125992094 1102 62-63 0.1957968059053954 1102 64-65 0.19188725421231823 1102 66-67 0.1350836594139011 1102 68-69 0.3089221391342143 1102 70-71 0.24970202842704836 1102 72-73 0.1612628556920619 1102 74-75 -0.009365349030538539 1102 76-77 0.11275889879370382 1102 78-79 0.13230636526921202 1102 80-81 0.04990446145816918 1102 82-83 0.02636661807402163 1102 84-85 -0.1721787259341987 1102 86-87 -0.151954536130539 1102 88-89 -0.09482180783004424 1102 90-91 -0.27630887689042183 1102 92-93 -0.425836134194725 1102 94-95 -0.344334060055111 1102 96-97 -0.4547077202374936 1102 98-99 -0.5179969392613373 1102 100-101 -0.17974448877362903 1103 1 0.20375320630057203 1103 2 0.32027079624665333 1103 3 0.41114562681555 1103 4 0.380177853302925 1103 5 0.4063441206204246 1103 6 0.3050392849910466 1103 7 0.32971489985192903 1103 8 0.3526134244610546 1103 9 0.4046597805596619 1103 10-11 0.4004368300004728 1103 12-13 0.4968373009465239 1103 14-15 0.564830861670977 1103 16-17 0.6243145903362333 1103 18-19 0.6218409955437778 1103 20-21 0.6164254652239123 1103 22-23 0.5440276444572234 1103 24-25 0.5805948048245781 1103 26-27 0.689011222723046 1103 28-29 0.6523183857837367 1103 30-31 0.7703182944844045 1103 32-33 0.724437058085968 1103 34-35 0.7389525548410134 1103 36-37 0.9094893388467611 1103 38-39 0.7615272282393803 1103 40-41 0.8766709211644468 1103 42-43 0.9420086023759353 1103 44-45 0.9247548763493185 1103 46-47 0.879336249263261 1103 48-49 0.681021439866818 1103 50-51 0.875881623087345 1103 52-53 0.7197969107168731 1103 54-55 0.8510231074284249 1103 56-57 0.8865565210550983 1103 58-59 0.8740362713245204 1103 60-61 0.9087483651333343 1103 62-63 0.6552184530274658 1103 64-65 0.598852975794486 1103 66-67 0.6093227807075365 1103 68-69 0.7419133672539964 1103 70-71 0.7484757410117453 1103 72-73 0.6826883857869035 1103 74-75 0.6278680936229435 1103 76-77 0.7568209907455881 1103 78-79 0.6906287391162103 1103 80-81 0.669377400299723 1103 82-83 0.7094139420002676 1103 84-85 0.62030462849998 1103 86-87 0.617674514482978 1103 88-89 0.6164464891782728 1103 90-91 0.5685794506460446 1103 92-93 0.3703187434093351 1103 94-95 0.6253446075248092 1103 96-97 0.48959735528701387 1103 98-99 0.5734406690276295 1103 100-101 0.8474519613273337 1104 1 0.058944147220927334 1104 2 0.18810596284398784 1104 3 0.18990860113526153 1104 4 0.370283257353762 1104 5 0.3400673221153596 1104 6 0.3156792182917556 1104 7 0.35145110510491406 1104 8 0.2808649409054027 1104 9 0.32576928940980565 1104 10-11 0.35736537264583035 1104 12-13 0.38645855525058437 1104 14-15 0.427270439108014 1104 16-17 0.4637377645526257 1104 18-19 0.44052886807191527 1104 20-21 0.5052758196378591 1104 22-23 0.4459515213475669 1104 24-25 0.4833431919289808 1104 26-27 0.4891456035681827 1104 28-29 0.40316351762903935 1104 30-31 0.6276438848932457 1104 32-33 0.49742532476241763 1104 34-35 0.5403045291033308 1104 36-37 0.5248892298509347 1104 38-39 0.5417975047467536 1104 40-41 0.5179312253231529 1104 42-43 0.5402707047882274 1104 44-45 0.5703934753091886 1104 46-47 0.5397478061342866 1104 48-49 0.5420360832960327 1104 50-51 0.5680711432749419 1104 52-53 0.5595084919078133 1104 54-55 0.5451442561095021 1104 56-57 0.4838373060143937 1104 58-59 0.49358137648300726 1104 60-61 0.473470168381084 1104 62-63 0.5007203042404669 1104 64-65 0.5503452185699658 1104 66-67 0.49930628931980436 1104 68-69 0.5395897798357332 1104 70-71 0.5540232852624527 1104 72-73 0.4770704853825798 1104 74-75 0.32723755100480645 1104 76-77 0.4273999289889794 1104 78-79 0.23640834624603002 1104 80-81 0.26548349193550536 1104 82-83 0.2238250598110234 1104 84-85 0.2893689863329172 1104 86-87 0.3856699203267375 1104 88-89 0.5377340482102788 1104 90-91 0.3391592764775808 1104 92-93 0.5352271176851389 1104 94-95 0.6169166030537312 1104 96-97 0.5304964110707466 1104 98-99 0.6503188203353751 1104 100-101 0.9632567108706702 1105 1 0.07609613005794458 1105 2 0.0954320337685104 1105 3 0.12370699451210854 1105 4 0.17508307123806333 1105 5 0.15892024659009252 1105 6 0.1615301503887494 1105 7 0.14258310457086054 1105 8 0.20585996998884326 1105 9 0.16473199235069558 1105 10-11 0.26796491388896726 1105 12-13 0.29796775989819935 1105 14-15 0.30303694871090414 1105 16-17 0.4063977406807666 1105 18-19 0.39032759609029455 1105 20-21 0.39285334158294916 1105 22-23 0.3707405510647632 1105 24-25 0.406091252125492 1105 26-27 0.35213453690403895 1105 28-29 0.5172135339313471 1105 30-31 0.5223998687594005 1105 32-33 0.5105281450914276 1105 34-35 0.49413608866465353 1105 36-37 0.6056757062074212 1105 38-39 0.5655957892505015 1105 40-41 0.5108265679038198 1105 42-43 0.46537684173569005 1105 44-45 0.571980100191503 1105 46-47 0.7950535647763743 1105 48-49 0.8432813623578426 1105 50-51 0.9316223775234391 1105 52-53 0.8457941485644582 1105 54-55 0.688783689029627 1105 56-57 0.5954352135880043 1105 58-59 0.7063202741485881 1105 60-61 0.7259069489232814 1105 62-63 0.6787667311685723 1105 64-65 0.5698610656227387 1105 66-67 0.4878518650321624 1105 68-69 0.6000829979011009 1105 70-71 0.6586585288602826 1105 72-73 0.7868585209164678 1105 74-75 0.5850183963299393 1105 76-77 0.6038911896306125 1105 78-79 0.668664007790504 1105 80-81 0.7493333708285093 1105 82-83 0.5810916802925732 1105 84-85 0.6937343392450686 1105 86-87 0.9623435147606862 1105 88-89 0.8604942563527267 1105 90-91 0.8490577322559041 1105 92-93 0.924434183434748 1105 94-95 0.9027808062086464 1105 96-97 0.929152401297376 1105 98-99 0.9204321228518886 1105 100-101 1.2377766234742218 1106 1 0.01354534807770591 1106 2 0.026608608462307615 1106 3 0.054138462455419756 1106 4 -0.0015178540357680959 1106 5 0.03651155952197627 1106 6 -0.030241865540062918 1106 7 0.0478512815728962 1106 8 -0.04251824407262461 1106 9 -0.018781757093549345 1106 10-11 -1.4189917621365566E-5 1106 12-13 0.053682486282326636 1106 14-15 0.17678983292815786 1106 16-17 0.2995903072780024 1106 18-19 0.17300811430474283 1106 20-21 0.06838408902396509 1106 22-23 0.17534112038742222 1106 24-25 0.10942620251314139 1106 26-27 0.04767658048215395 1106 28-29 0.07706380200708196 1106 30-31 -0.06581144006178619 1106 32-33 0.005446377594616081 1106 34-35 0.08917870485266377 1106 36-37 0.31519016157722035 1106 38-39 0.473009634622251 1106 40-41 0.5233112565245719 1106 42-43 0.55717738024466 1106 44-45 0.41963034097321383 1106 46-47 0.3424659624362647 1106 48-49 0.4567728416105723 1106 50-51 0.3969893575400292 1106 52-53 0.21375967177117872 1106 54-55 0.22473210134460686 1106 56-57 0.4684858247316015 1106 58-59 0.5569346717759416 1106 60-61 0.6303988070382189 1106 62-63 0.44988580912221465 1106 64-65 0.48773064471379257 1106 66-67 0.5405281563264026 1106 68-69 0.4676739754685393 1106 70-71 0.3660492307647907 1106 72-73 0.2899242832280784 1106 74-75 0.35907606374071577 1106 76-77 0.3049798623145783 1106 78-79 0.44399041534994765 1106 80-81 0.4425371605131616 1106 82-83 0.48297350649476556 1106 84-85 0.5543326360585432 1106 86-87 0.7276181201346859 1106 88-89 0.8373640708629857 1106 90-91 0.8861668043954545 1106 92-93 1.0786166595466753 1106 94-95 1.1244409332686018 1106 96-97 1.264301185236011 1106 98-99 1.2789005233859463 1106 100-101 1.7708402374611083 1107 1 0.08169356382919446 1107 2 -0.04532664670023934 1107 3 0.17411342927901785 1107 4 0.1469292321915745 1107 5 0.09628860929932159 1107 6 -0.08216907323018319 1107 7 0.029260350879532382 1107 8 -0.02019805293804211 1107 9 0.1290791133356919 1107 10-11 0.06891820026157802 1107 12-13 0.0784142928282634 1107 14-15 0.1641307123262692 1107 16-17 0.14737089995672648 1107 18-19 0.18595722405446935 1107 20-21 0.14924947792184895 1107 22-23 0.21098784488339817 1107 24-25 -0.02555920446692994 1107 26-27 0.10676553525511423 1107 28-29 0.1828275497086409 1107 30-31 0.036530928129671736 1107 32-33 0.03597342575788076 1107 34-35 0.18510147932000365 1107 36-37 0.1767789431774176 1107 38-39 -0.008889789918903546 1107 40-41 -0.008260245005310196 1107 42-43 -0.06457658792854204 1107 44-45 0.3380376264635885 1107 46-47 0.34643334226831257 1107 48-49 0.25222423598235366 1107 50-51 0.1666422230212916 1107 52-53 0.13836101602954898 1107 54-55 0.12490448621426964 1107 56-57 0.274879275660453 1107 58-59 0.23278671734297518 1107 60-61 0.19291083748423432 1107 62-63 0.1816219369441754 1107 64-65 0.21023953671122086 1107 66-67 0.03630865008052808 1107 68-69 0.10166368008778193 1107 70-71 0.20795185284666573 1107 72-73 0.07637375787169987 1107 74-75 0.1393123293895968 1107 76-77 0.0760706909645883 1107 78-79 0.03681194738340565 1107 80-81 0.017999285577715796 1107 82-83 -0.04110883378988461 1107 84-85 0.18053372460120443 1107 86-87 0.013183292022198145 1107 88-89 0.16852325159658577 1107 90-91 0.20656361241377752 1107 92-93 0.24528179419520058 1107 94-95 0.31826212902407747 1107 96-97 0.5400329892650646 1107 98-99 0.7434819054479647 1107 100-101 1.1378548801621982 1108 1 0.1525431009331477 1108 2 0.17497074557874726 1108 3 0.11634379363703218 1108 4 0.014261191731804956 1108 5 0.014045642256029112 1108 6 0.049918388628348964 1108 7 0.07369380454729679 1108 8 0.10880298991716586 1108 9 0.1000425312966513 1108 10-11 0.2979928964355594 1108 12-13 0.2368331805319741 1108 14-15 0.2633224448869882 1108 16-17 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Count 2 15903.0 3 3817.0 4 3616.0 5 4105.0 6 4686.0 7 5542.0 8 6967.0 9 8047.0 10 9753.0 11 12075.0 12 14983.0 13 18344.0 14 19976.0 15 22892.0 16 27886.0 17 34625.0 18 30669.0 19 21886.0 20 21970.0 21 23549.0 22 25057.0 23 28010.0 24 31616.0 25 35682.0 26 41539.0 27 47644.0 28 55089.0 29 65221.0 30 77817.0 31 93087.0 32 113621.0 33 142669.0 34 188870.0 35 265850.0 36 377309.0 37 524794.0 38 429123.0 39 62210.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.858651013486565 18.493241589120526 14.092366324024127 21.555741073368782 2 15.041690408063523 21.195494818794323 40.0728676275879 23.689947145554253 3 20.39673740702809 26.532015937109094 29.506017643233168 23.565229012629647 4 12.466079891579296 16.333204081163036 37.7143865908372 33.48632943642047 5 13.285863403598775 38.711341605799774 32.97638659133161 15.026408399269839 6 30.206727569871433 35.84271897961308 18.090766640361778 15.85978681015371 7 28.074198090947245 34.19923894987319 20.33432311324565 17.392239845933922 8 26.12531881397763 33.045592976811655 19.979100927396345 20.849987281814368 9 27.395153289604497 17.82374331095381 20.69977132845496 34.08133207098673 10-11 24.88083651947289 26.431692632522935 26.650443196235557 22.037027651768618 12-13 27.76864050629475 24.078740544384374 26.864351291808603 21.28826765751227 14-15 24.272380389073625 24.885423632872005 27.1377942465026 23.704401731551773 16-17 21.6066627258938 28.69682148814725 27.546208656701943 22.15030712925701 18-19 21.287610038554305 27.734802186645734 30.081518059485944 20.89606971531401 20-21 22.961773695840428 26.197961554668876 29.07836372597339 21.761901023517314 22-23 22.167242161302458 26.23253558107084 28.878917200440736 22.721305057185965 24-25 22.462772388980504 26.270031558318273 29.510120864715795 21.757075187985432 26-27 22.67479468909867 25.73252328370822 30.028035415184267 21.564646612008847 28-29 21.58101220953741 26.405818392955823 30.462065747578983 21.551103649927793 30-31 21.625517739537443 26.868689612815334 29.8198200211936 21.68597262645362 32-33 21.5000306616685 26.918935186386012 29.374410922356237 22.206623229589248 34-35 22.33487069505452 26.15064676562128 29.310176320545768 22.204306218778434 36-37 22.755936550560733 25.820186423610213 29.7372855827244 21.68659144310465 38-39 22.82988873413066 25.73914073099083 30.019703994339896 21.411266540538616 40-41 21.938500953428246 26.306740335141555 30.260542934549612 21.494215776880587 42-43 21.608228900277762 26.93285192591649 29.23648278876701 22.222436385038737 44-45 21.784676528411882 26.96690321935513 28.627103454697316 22.62131679753567 46-47 22.161881189822957 26.771756679070695 28.051511719570254 23.01485041153609 48-49 22.588036082601125 26.67668942014188 28.087225844036524 22.648048653220478 50-51 22.61571690004078 27.101998055153558 28.291318648233606 21.990966396572055 52-53 22.201071983168863 27.417664259636343 28.26187209223662 22.11939166495818 54-55 21.377719513920614 26.849520774802198 28.717140946015775 23.055618765261414 56-57 22.300216233611465 27.580945628389674 27.530126857625696 22.588711280373165 58-59 22.547641344605886 26.674077475585538 27.84235519889007 22.935925980918515 60-61 22.217293672246345 26.419473990900894 28.741308342546418 22.62192399430635 62-63 23.056104488159406 26.3993937626952 28.265711070715177 22.278790678430212 64-65 22.481900993917357 26.80536402029499 28.532039329721282 22.180695656066373 66-67 21.92426507327659 26.94079918126637 28.30750169507751 22.827434050379527 68-69 21.69378683451647 27.457620289641465 28.252732736290177 22.595860139551892 70-71 22.042473092729615 27.2982497102135 27.61045462904229 23.048822568014597 72-73 21.996172312070197 26.8958091362462 27.57791304234044 23.530105509343162 74-75 21.908093141254273 27.60635212707697 27.59374993005953 22.891804801609226 76-77 22.47741780452656 26.67485907429351 27.947183930166126 22.900539191013806 78-79 22.0225133274066 26.713048003444527 28.372705498817925 22.891733170330948 80-81 22.004824162462235 27.71411631171008 27.797760370099144 22.48329915572854 82-83 21.969973019609192 27.32300304228649 27.618470594816497 23.08855334328782 84-85 21.948203530611988 26.834461876781614 27.5701451761459 23.647189416460503 86-87 22.468737171935015 26.787039276146444 27.81111580606935 22.933107745849192 88-89 22.75943552525197 26.270284208281357 27.74350732342693 23.226772943039744 90-91 22.116820241587813 26.54349682520006 28.272884729281163 23.066798203930965 92-93 22.21206812693958 27.272279635520015 27.76994890909952 22.74570332844089 94-95 21.941194245979307 27.117109593793824 27.514870178114993 23.42682598211188 96-97 21.934821683312013 26.64266876088743 27.386264062940953 24.0362454928596 98-99 22.43727420832804 26.728826849231858 27.004193255460063 23.82970568698004 100-101 23.034055706237584 26.097480493625024 27.730320159476417 23.138143640660978 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5337.0 1 3539.5 2 2001.5 3 2210.5 4 1851.0 5 1817.0 6 2953.5 7 5408.0 8 7917.0 9 8425.0 10 6927.5 11 5614.0 12 5425.0 13 5490.5 14 5626.5 15 5769.5 16 5857.0 17 5964.0 18 6034.5 19 6197.5 20 6642.5 21 7464.0 22 8434.5 23 9655.0 24 10812.5 25 12458.5 26 15397.0 27 18678.5 28 21917.5 29 25583.0 30 29912.0 31 34350.5 32 39329.5 33 44593.5 34 50479.5 35 55294.5 36 61031.0 37 67798.5 38 70234.0 39 70526.5 40 71826.0 41 76049.0 42 80771.0 43 85624.0 44 91174.5 45 95827.0 46 99943.0 47 114064.5 48 175339.0 49 177451.5 50 121325.0 51 104925.5 52 94030.0 53 90471.5 54 88982.0 55 84029.5 56 77386.5 57 69413.5 58 62397.0 59 56640.0 60 49955.5 61 41897.5 62 34278.5 63 26820.0 64 19896.0 65 15324.5 66 11633.0 67 9236.0 68 7227.0 69 5093.5 70 3855.5 71 3181.5 72 2701.5 73 2322.5 74 1883.5 75 1528.0 76 1103.5 77 711.0 78 499.0 79 362.0 80 249.0 81 174.5 82 152.0 83 129.0 84 83.5 85 57.0 86 48.5 87 32.5 88 21.5 89 19.0 90 16.0 91 11.5 92 9.0 93 11.5 94 9.0 95 3.5 96 3.0 97 4.0 98 2.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2787924047499286 2 0.16890067467693373 3 0.19941663092185274 4 0.1675291710254767 5 0.24056174046556378 6 0.1977708265401043 7 0.24364762368134207 8 0.24985367770418518 9 0.15326553305032356 10-11 0.20965147692085087 12-13 0.21482890320510117 14-15 0.1758953432993646 16-17 0.28966157118772556 18-19 0.23469856235558495 20-21 0.20251965793327428 22-23 0.24928793244795916 24-25 0.23908737404024746 26-27 0.34915054206967444 28-29 0.31930319385484074 30-31 0.2932789120684435 32-33 0.19566213967598914 34-35 0.36560858588715883 36-37 0.4056907801009907 38-39 0.38602684649822544 40-41 0.34812191433108164 42-43 0.3818609041569247 44-45 0.39003849467873725 46-47 0.39557594067149504 48-49 0.41541131223069244 50-51 0.4082794932431158 52-53 0.4076966041912466 54-55 0.40164484432919245 56-57 0.3800608056143873 58-59 0.35629950485289424 60-61 0.32261194641398083 62-63 0.3899870632918076 64-65 0.4231774516570678 66-67 0.4289377669931874 68-69 0.43342944145170914 70-71 0.43356659181685486 72-73 0.42530328231682624 74-75 0.4200744246456463 76-77 0.4040792633105287 78-79 0.3781235567067043 80-81 0.388804141392426 82-83 0.3984046669526252 84-85 0.3996733078302229 86-87 0.39429015599825407 88-89 0.40299920418500623 90-91 0.42472039326495703 92-93 0.42412036041744455 94-95 0.4328636961954831 96-97 0.43348087283863884 98-99 0.4305835713749358 100-101 0.4029306290024334 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2916507.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.69419765714265 #Duplication Level Percentage of deduplicated Percentage of total 1 68.4020674247513 26.467631170905253 2 14.133836434439845 10.937949212958795 3 6.314830526889245 7.330419017364323 4 3.3608991704959927 5.201891872355949 5 2.0088566183127394 3.8865547526926156 6 1.3064592854167816 3.0331436292555782 7 0.8397238812932315 2.2744709288128306 8 0.6368696662804103 1.9714528599114145 9 0.46208719631676537 1.6092083978204224 >10 2.248047254486706 15.278041006775753 >50 0.1659162521492463 4.426693684180208 >100 0.10865017885541385 7.83531822188364 >500 0.006234300923155021 1.6674250148808216 >1k 0.004364010646199974 3.408184336497651 >5k 6.23430092314282E-4 1.580547295285327 >10k+ 5.343686505550988E-4 3.091068598419516 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19375 0.6643220811745009 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18485 0.6338061249295818 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 15895 0.5450012634977389 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13253 0.45441344731900185 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11484 0.3937586983333145 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11204 0.38415817277311526 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9343 0.32034896538907676 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6907 0.23682439301534333 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6559 0.22489231124766715 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6444 0.22094923824972817 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6421 0.2201606236501404 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5119 0.17551817979521392 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5071 0.17387237541346548 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4895 0.16783775934705455 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4752 0.16293463379309564 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4538 0.1555970892578005 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3514 0.12048659578050044 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3340 0.11452055489666235 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3308 0.11342335197549673 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3103 0.10639439576177942 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.4287591286425854E-5 0.0 0.0 0.0 0.0 6 3.4287591286425854E-5 0.0 0.0 0.0 0.0 7 2.4001313900498097E-4 0.0 0.0 0.0 0.0 8 2.4001313900498097E-4 0.0 0.0 0.0 0.0 9 2.4001313900498097E-4 0.0 0.0 0.0 0.0 10-11 2.4001313900498097E-4 0.0 0.0 1.7143795643212927E-5 3.4287591286425854E-5 12-13 3.0858832157783266E-4 0.0 0.0 6.857518257285171E-5 1.0286277385927755E-4 14-15 3.0858832157783266E-4 0.0 0.0 6.857518257285171E-5 1.0286277385927755E-4 16-17 3.257321172210456E-4 0.0 0.0 3.257321172210456E-4 1.0286277385927755E-4 18-19 3.7716350415068433E-4 0.0 0.0 4.2859489108032316E-4 1.0286277385927755E-4 20-21 4.114510954371102E-4 0.0 0.0 7.028956213717299E-4 1.0286277385927755E-4 22-23 4.114510954371102E-4 0.0 0.0 0.0017658109512509314 1.0286277385927755E-4 24-25 6.514642344420912E-4 0.0 0.0 0.0031201708070647525 1.0286277385927755E-4 26-27 6.514642344420912E-4 0.0 0.0 0.00545172701454171 1.3715036514570341E-4 28-29 8.743335778038592E-4 0.0 0.0 0.010749159868294503 1.3715036514570341E-4 30-31 9.600525560199239E-4 0.0 0.0 0.025287098573739064 1.5429416078891633E-4 32-33 9.771963516631368E-4 0.0 0.0 0.05139709933835235 1.7143795643212925E-4 34-35 0.0011143467168088401 0.0 0.0 0.09146214975654096 1.7143795643212925E-4 36-37 0.0011657781037384789 0.0 0.0 0.1536084089631878 1.7143795643212925E-4 38-39 0.0012686408775977565 0.0 0.0 0.23639579812426303 1.7143795643212925E-4 40-41 0.0012857846732409694 0.0 0.0 0.3326239230696172 1.7143795643212925E-4 42-43 0.001422935038386673 0.0 0.0 0.42895491078883063 1.7143795643212925E-4 44-45 0.001662948177391654 0.0 0.0 0.538246608014313 1.7143795643212925E-4 46-47 0.0017486671556077183 0.0 0.0 0.6807458374006988 1.8858175207534216E-4 48-49 0.0017829547468941442 0.0 0.0 0.8373201230101626 2.057255477185551E-4 50-51 0.0017829547468941442 0.0 0.0 1.0054664706787948 2.057255477185551E-4 52-53 0.0017829547468941442 0.0 0.0 1.1668067314770716 2.057255477185551E-4 54-55 0.0017829547468941442 3.4287591286425854E-5 0.0 1.3396504791519446 2.057255477185551E-4 56-57 0.00181724233818057 3.4287591286425854E-5 0.0 1.5478104458518358 2.057255477185551E-4 58-59 0.00181724233818057 3.4287591286425854E-5 0.0 1.7953497111441872 2.057255477185551E-4 60-61 0.001851529929466996 3.4287591286425854E-5 0.0 2.0887829173734196 2.057255477185551E-4 62-63 0.001851529929466996 3.4287591286425854E-5 0.0 2.3605635097052744 2.057255477185551E-4 64-65 0.0018686737251102088 3.4287591286425854E-5 0.0 2.619897706400156 2.057255477185551E-4 66-67 0.002057255477185551 3.4287591286425854E-5 0.0 2.92358290242403 2.2286934336176805E-4 68-69 0.002057255477185551 3.4287591286425854E-5 0.0 3.2669731291575848 2.4001313900498097E-4 70-71 0.00210868686411519 3.4287591286425854E-5 0.0 3.657954532596699 2.4001313900498097E-4 72-73 0.002194405842331254 3.4287591286425854E-5 0.0 4.054061930933133 2.4001313900498097E-4 74-75 0.002314412411833745 3.4287591286425854E-5 0.0 4.448797825618111 2.4001313900498097E-4 76-77 0.0023315562074769577 3.4287591286425854E-5 0.0 4.853219964841504 2.4001313900498097E-4 78-79 0.0023315562074769577 6.857518257285171E-5 0.0 5.321382736266362 2.4001313900498097E-4 80-81 0.0024515627769794487 6.857518257285171E-5 0.0 5.834650834028515 2.4001313900498097E-4 82-83 0.002588713142125152 6.857518257285171E-5 0.0 6.349976187267851 2.7430073029140683E-4 84-85 0.0026230007334115775 6.857518257285171E-5 0.0 6.907423846402564 2.7430073029140683E-4 86-87 0.0026572883246980036 6.857518257285171E-5 0.0 7.482203882932563 2.7430073029140683E-4 88-89 0.002674432120341216 6.857518257285171E-5 0.0 8.059572632604688 2.7430073029140683E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 215 1.6916601E-9 24.268845 2 TACCTGG 2290 0.0 24.027988 2 GAGTACT 13400 0.0 22.810635 12-13 GTACATG 23230 0.0 22.376661 1 TACATGG 23650 0.0 21.78179 2 GTACTTT 14455 0.0 21.162579 14-15 AGTACTT 13860 0.0 20.940619 12-13 ACATGGG 24080 0.0 20.723072 3 CATGGGG 7060 0.0 20.425081 4 TATCACG 170 8.043951E-5 19.531878 2 ATGGGAT 5485 0.0 19.285154 5 GTATAGG 800 0.0 19.005844 1 ACTTTTT 16585 0.0 18.874125 16-17 ATGGGGA 3465 0.0 18.207207 5 CATGGGA 16310 0.0 18.147863 4 TACTTTT 15975 0.0 18.079376 14-15 GCTTATA 2505 0.0 18.019514 1 GACCGGT 295 6.4501364E-8 17.687159 7 GTGTAAG 980 0.0 16.484661 1 GTACCTG 3610 0.0 16.189188 1 >>END_MODULE