##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938712_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2916507 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.068053325433475 33.0 31.0 34.0 30.0 34.0 2 32.25594212528892 34.0 31.0 34.0 30.0 34.0 3 32.33208835089372 34.0 31.0 34.0 30.0 34.0 4 35.94210197335374 37.0 35.0 37.0 35.0 37.0 5 35.85351449525066 37.0 35.0 37.0 35.0 37.0 6 35.896086654343705 37.0 35.0 37.0 35.0 37.0 7 35.81812318640072 37.0 35.0 37.0 33.0 37.0 8 35.81120909361781 37.0 35.0 37.0 33.0 37.0 9 37.44081464573889 39.0 37.0 39.0 34.0 39.0 10-11 37.36728336328355 39.0 37.0 39.0 34.0 39.0 12-13 37.28649648363607 39.0 37.0 39.0 33.5 39.0 14-15 38.442575656427366 40.0 38.0 41.0 33.5 41.0 16-17 38.38512405421966 40.0 38.0 41.0 33.0 41.0 18-19 38.5833236470888 40.0 38.0 41.0 34.0 41.0 20-21 38.59532430403904 40.0 38.0 41.0 34.0 41.0 22-23 38.52393291015588 40.0 38.0 41.0 34.0 41.0 24-25 38.431573282697414 40.0 38.0 41.0 34.0 41.0 26-27 38.21676186616388 40.0 38.0 41.0 33.5 41.0 28-29 38.03774120891875 40.0 37.0 41.0 33.0 41.0 30-31 37.811496252194836 40.0 37.0 41.0 33.0 41.0 32-33 37.644259382885075 40.0 36.5 41.0 33.0 41.0 34-35 37.59760374310777 40.0 36.0 41.0 33.0 41.0 36-37 37.45313709173337 40.0 36.0 41.0 32.0 41.0 38-39 37.298388277483994 40.0 36.0 41.0 31.5 41.0 40-41 37.10306781365517 40.0 36.0 41.0 31.0 41.0 42-43 36.89348028309207 40.0 35.5 41.0 31.0 41.0 44-45 36.61702543487809 39.5 35.0 41.0 30.5 41.0 46-47 36.230486503204006 39.0 35.0 41.0 30.0 41.0 48-49 35.87692109088029 39.0 35.0 41.0 28.5 41.0 50-51 35.62808575463731 39.0 35.0 41.0 28.0 41.0 52-53 35.365752593770566 39.0 35.0 40.0 27.0 41.0 54-55 35.09331247962031 38.0 34.5 40.0 26.5 41.0 56-57 34.83946772629039 38.0 34.0 40.0 26.0 41.0 58-59 34.54847082486001 38.0 34.0 40.0 26.0 41.0 60-61 34.15289145542938 37.5 33.5 40.0 24.5 41.0 62-63 33.771851396207865 37.0 33.0 40.0 23.5 41.0 64-65 33.330647414869915 36.0 33.0 39.0 22.5 41.0 66-67 32.91376825085625 36.0 32.0 39.0 22.0 40.0 68-69 32.58490293354345 35.0 32.0 38.5 21.5 40.0 70-71 32.132641546891534 35.0 31.5 38.0 20.0 40.0 72-73 31.638263168920904 35.0 31.0 37.0 18.5 39.0 74-75 31.153708357291787 35.0 31.0 36.5 17.0 39.0 76-77 29.206861838493786 32.5 28.5 35.0 11.5 36.5 78-79 30.146907927873993 34.0 30.0 35.0 9.5 37.0 80-81 30.21795061695377 34.0 30.0 35.0 7.5 37.0 82-83 29.917540571649578 34.0 30.0 35.0 6.0 37.0 84-85 29.53816980380983 34.0 30.0 35.0 2.0 36.0 86-87 28.9636873835722 34.0 29.0 35.0 2.0 36.0 88-89 28.346238325503762 34.0 28.5 35.0 2.0 35.0 90-91 27.813411385606138 33.0 27.0 35.0 2.0 35.0 92-93 27.546977771697446 33.0 27.0 35.0 2.0 35.0 94-95 27.227536570287675 33.0 26.5 35.0 2.0 35.0 96-97 26.602898775830127 33.0 25.0 35.0 2.0 35.0 98-99 25.76713239501911 33.0 24.0 35.0 2.0 35.0 100-101 22.085860585968078 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05369316485027298 1101 2 0.10200597813322787 1101 3 0.057651612571838484 1101 4 0.04091668131832904 1101 5 -0.08799975341234045 1101 6 -0.02508137203583516 1101 7 0.11815592434301436 1101 8 0.19452588925302194 1101 9 0.12706672666123353 1101 10-11 0.11502210983524463 1101 12-13 0.057251163990152065 1101 14-15 0.1510126156277849 1101 16-17 0.22699806011372914 1101 18-19 0.21323392542954167 1101 20-21 0.15196473561388046 1101 22-23 0.11803606286821378 1101 24-25 0.08006828407567212 1101 26-27 0.07286452218323092 1101 28-29 0.13311688526894017 1101 30-31 0.17154804781520028 1101 32-33 0.1912357435173888 1101 34-35 0.18941588040590318 1101 36-37 0.1956425432277058 1101 38-39 0.31788687761684997 1101 40-41 0.29750477403261755 1101 42-43 0.3124014234866195 1101 44-45 0.38259273548116823 1101 46-47 0.40373091250748416 1101 48-49 0.44262642725649215 1101 50-51 0.44386932486677466 1101 52-53 0.26269749161385647 1101 54-55 0.42253270463463366 1101 56-57 0.40421701067241145 1101 58-59 0.32958875491961237 1101 60-61 0.48298101895666434 1101 62-63 0.31099008511313997 1101 64-65 0.25707086723592454 1101 66-67 0.2639247109065863 1101 68-69 0.21950062921573732 1101 70-71 0.12584322969281203 1101 72-73 0.15867742696297427 1101 74-75 0.1455308453287003 1101 76-77 0.1826761321997843 1101 78-79 0.07631090734006918 1101 80-81 0.12815936099472225 1101 82-83 0.1271280626920408 1101 84-85 0.21115390066419693 1101 86-87 0.2262662845867851 1101 88-89 0.19339728488737506 1101 90-91 0.31889780588953087 1101 92-93 0.4790461921806113 1101 94-95 0.4684061910331714 1101 96-97 0.2905052775732777 1101 98-99 0.0652783459375108 1101 100-101 0.6635008974919749 1102 1 0.2374143795552115 1102 2 0.16744654726274888 1102 3 0.14047635623828825 1102 4 0.10994446683382364 1102 5 -0.10978102029233128 1102 6 0.007188315325642236 1102 7 0.1609211777941013 1102 8 0.1160248440564402 1102 9 0.18014733154301865 1102 10-11 0.21861435236929339 1102 12-13 0.27334767572227037 1102 14-15 0.2846770893598034 1102 16-17 0.31491731958650604 1102 18-19 0.2987812668999865 1102 20-21 0.31276841063646543 1102 22-23 0.24540658808251692 1102 24-25 0.24348446609248242 1102 26-27 0.22083226279038826 1102 28-29 0.3095506697041088 1102 30-31 0.22242671538940328 1102 32-33 0.28594393625986925 1102 34-35 0.42774801069334956 1102 36-37 0.4479337400807353 1102 38-39 0.45385980666216597 1102 40-41 0.43576811270295224 1102 42-43 0.5204731750810581 1102 44-45 0.5009958252905591 1102 46-47 0.4691222496082119 1102 48-49 0.36208428149093663 1102 50-51 0.42418390118065474 1102 52-53 0.3260612321338172 1102 54-55 0.40838482445799684 1102 56-57 0.385134966980047 1102 58-59 0.4465597558618555 1102 60-61 0.4664256138491325 1102 62-63 0.34985753044923484 1102 64-65 0.34909122131896453 1102 66-67 0.5313633252883889 1102 68-69 0.47236753197006465 1102 70-71 0.47581131668029286 1102 72-73 0.3856246657304432 1102 74-75 0.36514523682566846 1102 76-77 0.4771391336943047 1102 78-79 0.36360074773867623 1102 80-81 0.5778304207974081 1102 82-83 0.5095739680163653 1102 84-85 0.6293443177981537 1102 86-87 0.675494074462307 1102 88-89 0.5783512381042328 1102 90-91 0.6676135342207772 1102 92-93 0.7903552070313644 1102 94-95 0.842971035610141 1102 96-97 0.8807690231799405 1102 98-99 0.8697150419052093 1102 100-101 1.6810960491363112 1103 1 0.2032134431131709 1103 2 0.20930418530986827 1103 3 0.212684683009563 1103 4 0.12677958534726486 1103 5 0.007278084414991781 1103 6 0.076768410692722 1103 7 0.11712421898689485 1103 8 0.09780997925124524 1103 9 0.15725506905867803 1103 10-11 0.1763454252832517 1103 12-13 0.20668630682578026 1103 14-15 0.28120053295533154 1103 16-17 0.22227850908469549 1103 18-19 0.07881261558274133 1103 20-21 0.23459455803182294 1103 22-23 0.2831931444111859 1103 24-25 0.2537379974814158 1103 26-27 0.28114767702769683 1103 28-29 0.22547048512514323 1103 30-31 0.26669229224739155 1103 32-33 0.306991323470605 1103 34-35 0.30961878448341196 1103 36-37 0.29089718438370227 1103 38-39 0.24484869662450848 1103 40-41 0.29009683809914577 1103 42-43 0.4027344839655669 1103 44-45 0.520671346692815 1103 46-47 0.6052014721666481 1103 48-49 0.6543836176173627 1103 50-51 0.5917884255646157 1103 52-53 0.4779238595494988 1103 54-55 0.5207978729501619 1103 56-57 0.39189010520553325 1103 58-59 0.5565414045624237 1103 60-61 0.6106216138602036 1103 62-63 0.5371230785524901 1103 64-65 0.6133235846470271 1103 66-67 0.7103285588821606 1103 68-69 0.6575643629811054 1103 70-71 0.7116793543184414 1103 72-73 0.6214440368726919 1103 74-75 0.695786312334775 1103 76-77 0.760997810521463 1103 78-79 0.614296185347925 1103 80-81 0.6812167065668291 1103 82-83 0.711246844223119 1103 84-85 0.6880362923260286 1103 86-87 0.7141349112709747 1103 88-89 0.6801452451591317 1103 90-91 0.6976764950711392 1103 92-93 0.6885355132104003 1103 94-95 0.813851567122871 1103 96-97 0.8487976017825893 1103 98-99 0.8503087202176296 1103 100-101 1.4173332062044501 1104 1 0.09730772745972871 1104 2 0.05226806698134823 1104 3 0.04666830286621604 1104 4 0.09910636216559254 1104 5 -0.023947621557823595 1104 6 0.01504979151951602 1104 7 0.08751356840253521 1104 8 0.0010822800513210495 1104 9 0.14895174113568999 1104 10-11 0.12531058626510827 1104 12-13 0.02292467276416943 1104 14-15 0.08384371127829837 1104 16-17 0.1567376986693816 1104 18-19 0.225779046407375 1104 20-21 0.22294733053173843 1104 22-23 0.17003180988099587 1104 24-25 0.24514732768964365 1104 26-27 0.15119394403905773 1104 28-29 0.09929551073206255 1104 30-31 -0.05223889777753499 1104 32-33 0.13344902427410688 1104 34-35 0.23611743268667595 1104 36-37 0.2445775094539826 1104 38-39 0.16270407064528314 1104 40-41 0.18971209822460366 1104 42-43 0.14085414623236403 1104 44-45 0.24243536304100388 1104 46-47 0.2544263414397179 1104 48-49 0.24362474750523688 1104 50-51 0.11951590996821437 1104 52-53 0.2070578161275023 1104 54-55 0.1985643816458662 1104 56-57 0.13788172755689487 1104 58-59 0.33912432156760985 1104 60-61 0.4785749480258943 1104 62-63 0.5218081001475099 1104 64-65 0.28219178549993984 1104 66-67 0.2610241617113118 1104 68-69 0.3026605272393539 1104 70-71 0.1841982052113238 1104 72-73 0.47453648272833604 1104 74-75 0.4198321402858731 1104 76-77 0.36951569808534757 1104 78-79 0.22100688427625315 1104 80-81 0.38059202203045217 1104 82-83 0.4957077793978222 1104 84-85 0.5748825910538784 1104 86-87 0.6286518887611479 1104 88-89 0.4403990480305353 1104 90-91 0.4574576276691147 1104 92-93 0.5710480148894206 1104 94-95 0.5506404388977444 1104 96-97 0.6324808913972753 1104 98-99 0.9243061154422847 1104 100-101 1.6546017897853744 1105 1 0.07621132949760323 1105 2 0.07437136921708287 1105 3 0.042328506481830175 1105 4 0.02348826378900526 1105 5 -0.08947879908917145 1105 6 -0.05191083251817474 1105 7 0.07077083381967952 1105 8 0.07355474804978712 1105 9 0.09344983900802362 1105 10-11 0.09124018204220619 1105 12-13 0.10289505928086129 1105 14-15 0.16059260005641107 1105 16-17 0.13268614095317588 1105 18-19 0.10722048041508714 1105 20-21 0.19118990667558222 1105 22-23 0.08425940394019449 1105 24-25 0.05433575894860354 1105 26-27 0.054260841975114715 1105 28-29 0.06517416535850629 1105 30-31 0.08729842592682502 1105 32-33 0.16749427308065634 1105 34-35 0.21925018165796217 1105 36-37 0.18020371028130455 1105 38-39 0.21512354609468076 1105 40-41 0.1145157398579073 1105 42-43 0.24400132375453154 1105 44-45 0.1443467735722379 1105 46-47 0.057568304513395674 1105 48-49 0.06900650530155872 1105 50-51 0.10321709112675137 1105 52-53 0.18061574893675214 1105 54-55 0.135268598071832 1105 56-57 0.14886135052361738 1105 58-59 0.17058446455810383 1105 60-61 0.1446099215038359 1105 62-63 0.09801485818736211 1105 64-65 0.02297501858407003 1105 66-67 0.1641535184260121 1105 68-69 0.17424172565714002 1105 70-71 0.10146255654825609 1105 72-73 0.3240512811612497 1105 74-75 0.17544853107383318 1105 76-77 0.28033854378752565 1105 78-79 0.1485954425156244 1105 80-81 0.29865853891065086 1105 82-83 0.4157716592569294 1105 84-85 0.37258427990487064 1105 86-87 0.42964647318392934 1105 88-89 0.3929078567238804 1105 90-91 0.3613400424286404 1105 92-93 0.5149576823050204 1105 94-95 0.5437280857546618 1105 96-97 0.37269351483215374 1105 98-99 0.6725610228972876 1105 100-101 1.1671832225668375 1106 1 0.1738492016400741 1106 2 0.0859543923383086 1106 3 0.07108916218905392 1106 4 0.02935946591116334 1106 5 -0.03747107840192854 1106 6 -0.028382858308695802 1106 7 -0.06259397728546645 1106 8 0.05724607462868647 1106 9 0.027541095990493147 1106 10-11 0.11517657037963147 1106 12-13 0.03427019629052808 1106 14-15 0.07858005476975194 1106 16-17 0.1019971396784527 1106 18-19 0.018756652126995732 1106 20-21 -0.04370150995364952 1106 22-23 0.08171378856469858 1106 24-25 0.07126386046375188 1106 26-27 0.12365770728046499 1106 28-29 0.15300910229836973 1106 30-31 -0.0052374240344406076 1106 32-33 0.08873658916728999 1106 34-35 0.04564429332237552 1106 36-37 0.04453019719927198 1106 38-39 0.1784786714761637 1106 40-41 0.27810585278275823 1106 42-43 0.1877729917012303 1106 44-45 0.17316736322262472 1106 46-47 0.20746724692003227 1106 48-49 0.120371222770288 1106 50-51 0.13983540222476165 1106 52-53 0.18373231398135204 1106 54-55 0.2610975044284487 1106 56-57 0.10987573168158349 1106 58-59 0.2551789840389631 1106 60-61 0.1993425613300559 1106 62-63 0.1971386272587239 1106 64-65 0.24847440446867353 1106 66-67 0.15440470639645554 1106 68-69 0.21210038714126256 1106 70-71 0.2638279251552902 1106 72-73 0.18011120049447982 1106 74-75 0.3362220807139167 1106 76-77 0.29867467791205016 1106 78-79 0.16524894363743314 1106 80-81 0.1280662819776559 1106 82-83 0.18193066384212742 1106 84-85 0.29666590429071604 1106 86-87 0.3932655735505364 1106 88-89 0.3466190959818434 1106 90-91 0.307799772629604 1106 92-93 0.3975085705393404 1106 94-95 0.6974837192962902 1106 96-97 0.7894128934848439 1106 98-99 1.0268539700501584 1106 100-101 1.5606917290362112 1107 1 0.012927329421984268 1107 2 0.01393071784313804 1107 3 -0.07579121663088273 1107 4 -0.025414559771803624 1107 5 -0.022051534687271612 1107 6 -0.03698076296748809 1107 7 0.006073045465988969 1107 8 -0.02843510386095005 1107 9 -0.05610063806582133 1107 10-11 -0.10866080020450397 1107 12-13 0.04314189447682537 1107 14-15 0.06955739808177697 1107 16-17 -0.046869500659383334 1107 18-19 -0.01204473671310069 1107 20-21 -0.04275609061675567 1107 22-23 -0.014258338772712875 1107 24-25 -0.011852664945372737 1107 26-27 -0.07987314586869587 1107 28-29 -0.007708136068224292 1107 30-31 -0.027858930308106267 1107 32-33 -0.04792085146727487 1107 34-35 -0.07061892183874363 1107 36-37 -0.07530415224509568 1107 38-39 -0.04941761702386316 1107 40-41 -0.21427711717228703 1107 42-43 -0.11685792707303477 1107 44-45 -0.09318022783752156 1107 46-47 -0.09941158018438756 1107 48-49 -0.0930027922104415 1107 50-51 -0.26271379935851513 1107 52-53 -0.23718704353839115 1107 54-55 -0.1435132464212998 1107 56-57 -0.16731619506431628 1107 58-59 -0.199285770953594 1107 60-61 -0.1471773992795704 1107 62-63 -0.17660162978985028 1107 64-65 -0.2458122074545983 1107 66-67 -0.1798483849699366 1107 68-69 -0.2018011235708883 1107 70-71 -0.2100753495663099 1107 72-73 -0.2564968982130118 1107 74-75 -0.19911643456134698 1107 76-77 -0.3008578595816296 1107 78-79 -0.23675923668285037 1107 80-81 -0.1660128618605725 1107 82-83 -0.10341012210287559 1107 84-85 -0.1724890443522078 1107 86-87 -0.032089742492217965 1107 88-89 -0.0839281748208549 1107 90-91 0.1188044340136507 1107 92-93 0.10814153149377503 1107 94-95 0.2377246926180554 1107 96-97 0.4591480830551298 1107 98-99 0.7140988137968911 1107 100-101 1.4761071472448855 1108 1 0.10930034072772088 1108 2 0.05325337938062802 1108 3 0.03543974846808595 1108 4 -0.0037435525310911544 1108 5 -0.06449425963658229 1108 6 0.08036413205831394 1108 7 -0.040212186727799804 1108 8 0.051669074774579826 1108 9 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8155.0 13 11269.0 14 13641.0 15 18184.0 16 23898.0 17 32917.0 18 30511.0 19 19549.0 20 18092.0 21 18901.0 22 19970.0 23 22313.0 24 25389.0 25 30010.0 26 36053.0 27 43327.0 28 52527.0 29 63841.0 30 76653.0 31 93061.0 32 114227.0 33 144444.0 34 191754.0 35 272482.0 36 404299.0 37 603197.0 38 471655.0 39 41582.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49887112002285 19.312388534057668 14.681420068801703 21.50732027711778 2 15.486676356339965 21.92184006415894 38.955572539342434 23.635911040158657 3 20.472057841794996 26.5065367578408 29.775961449775362 23.245443950588836 4 12.923164593810336 16.99323882987423 37.10085386388581 32.98274271242963 5 13.258443074668925 38.96984679885473 32.97683119063919 14.794878935837161 6 30.349738254363885 36.311476449750565 17.50851483396717 15.830270461918383 7 28.04632390733161 34.27600208057104 20.117318422345633 17.560355589751715 8 26.24807689472372 33.49163914230276 19.652824423188424 20.607459539785093 9 27.04546911768084 17.842679616404144 20.517008873971502 34.594842391943516 10-11 24.950463002488938 26.652173301829897 26.421212086924527 21.976151608756638 12-13 27.682995446265004 24.17887219197485 26.816393720296233 21.321738641463917 14-15 24.11135992473188 25.112128995404436 27.008849970186937 23.767661109676748 16-17 21.51118753675317 28.911523222860946 27.529192518897343 22.048096721488537 18-19 21.23649968952586 27.839364006326743 30.039787320928767 20.884348983218622 20-21 22.779149852889088 26.28795336338984 29.161270656987963 21.77162612673311 22-23 22.101147022791302 26.33955618827591 28.886027017936183 22.67326977099661 24-25 22.45379001826335 26.449748868205397 29.49058913665763 21.605871976873622 26-27 22.66165009811522 25.83464799385833 30.102394367984026 21.401307540042428 28-29 21.555014268781115 26.50787397390097 30.447535356506943 21.489576400810968 30-31 21.59053621335385 26.97701394167749 29.827272830135502 21.605177014833153 32-33 21.483370346788128 26.983357146065483 29.408295608411024 22.124976898735373 34-35 22.283227965837547 26.231174354087592 29.380043418014807 22.105554262060053 36-37 22.760936571929456 25.926252985257868 29.71228355594452 21.600526886868156 38-39 22.80069092067546 25.825680727297918 30.077630843017182 21.29599750900944 40-41 21.92525187338384 26.339538454733084 30.278830976937137 21.456378694945936 42-43 21.59188597265766 27.02842968257882 29.195383301277687 22.184301043485835 44-45 21.857557188120786 27.0101157057892 28.588119861932782 22.54420724415723 46-47 22.200419030449055 26.95256239982623 27.963193484506828 22.883825085217886 48-49 22.608712796260534 26.80755677049983 28.00088806368685 22.58284236955279 50-51 22.579535238398275 27.249626779440877 28.1881299061274 21.982708076033443 52-53 22.265693173374864 27.474972629930257 28.152238276815382 22.107095919879498 54-55 21.39550839411666 26.959681564282207 28.546717014565708 23.098093027035425 56-57 22.294903500387882 27.71960894405466 27.360423801094647 22.62506375446281 58-59 22.560850062189427 26.791685560652052 27.69209932821703 22.955365048941495 60-61 22.179908377849735 26.591510969987496 28.522378952793215 22.706201699369554 62-63 23.05180850686298 26.55229479504492 28.083146792855292 22.31274990523681 64-65 22.414431867373573 26.936382689645416 28.274767805174932 22.374417637806072 66-67 21.8744808353337 27.04492800659456 28.111589049781 22.96900210829074 68-69 21.61350934339105 27.570666895251154 28.04020229727413 22.775621464083663 70-71 21.92686108887178 27.359099332934456 27.463265106694486 23.250774471499277 72-73 21.84915037063172 26.926422600734373 27.41423216196635 23.81019486666756 74-75 21.711361065170255 27.63465965870293 27.358694513596216 23.295284762530596 76-77 22.130371245105277 26.627830800322016 27.93583522129119 23.305962733281515 78-79 21.645875088460496 26.639115865477557 28.321298814655137 23.393710231406814 80-81 21.570559004826883 27.61929416565017 27.705202594568277 23.104944234954665 82-83 21.497783973979608 27.208208382565026 27.57582594372261 23.71818169973276 84-85 21.34189974514033 26.703621832555175 27.5195807861939 24.434897636110595 86-87 21.795933428011207 26.619424982087004 27.77246418473341 23.812177405168384 88-89 22.01173060184756 26.07337966137096 27.765647411988226 24.149242324793256 90-91 21.329125743205623 26.334534395500274 28.26975621048392 24.06658365081018 92-93 21.18900194119897 26.95470404301874 27.93723565243435 23.919058363347943 94-95 20.844283977856982 26.74293972730967 27.685680928648942 24.72709536618441 96-97 20.69954656320488 26.355720128571765 27.597292442817228 25.347440865406128 98-99 21.0498551863582 26.383838612422327 27.270670017250087 25.295636183969382 100-101 21.39904340114285 25.77546860023141 28.089629325229037 24.735858673396702 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3307.0 1 2182.0 2 1358.5 3 1700.0 4 1494.5 5 1437.0 6 2446.0 7 4842.0 8 7155.0 9 7737.0 10 6494.5 11 5140.0 12 4985.5 13 5093.5 14 5131.0 15 5296.0 16 5314.5 17 5310.0 18 5558.5 19 5860.5 20 6276.5 21 7004.5 22 7995.0 23 9201.0 24 10367.0 25 12130.0 26 15072.0 27 18210.0 28 21275.5 29 25202.0 30 29567.5 31 34015.5 32 39474.5 33 44998.0 34 50724.5 35 55479.5 36 61419.5 37 68265.0 38 70836.0 39 70984.5 40 71996.0 41 76250.0 42 81166.5 43 86310.5 44 92220.5 45 96918.0 46 99771.5 47 113832.0 48 177847.5 49 180717.0 50 124718.5 51 107848.5 52 94600.0 53 90482.0 54 89304.0 55 84637.0 56 77991.0 57 70132.0 58 63531.5 59 57588.0 60 50370.0 61 41811.5 62 33983.0 63 26254.0 64 19227.5 65 14827.5 66 11092.5 67 8652.0 68 6641.5 69 4560.0 70 3431.5 71 2816.5 72 2373.0 73 2170.0 74 1802.5 75 1397.0 76 987.5 77 562.0 78 328.5 79 226.0 80 149.5 81 101.0 82 75.5 83 55.0 84 35.5 85 13.0 86 6.5 87 3.5 88 1.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.239533112726971 2 0.0 3 0.0 4 0.0 5 0.02959019128018551 6 0.004320236502089657 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0031544583983511786 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.6001970850747144E-4 26-27 0.0013029284688841824 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0070632438050037254 36-37 0.01752095914736361 38-39 0.01364646133199749 40-41 0.008914773734470721 42-43 0.007680420448159391 44-45 0.003480190515572224 46-47 4.2859489108032316E-4 48-49 0.0016972357686780798 50-51 3.4287591286425854E-5 52-53 0.0 54-55 0.0 56-57 0.0015257978122459504 58-59 4.971700736531748E-4 60-61 0.002485850368265874 62-63 9.08621169090285E-4 64-65 0.0015257978122459504 66-67 0.005606021175330626 68-69 2.4001313900498097E-4 70-71 6.857518257285171E-5 72-73 0.0 74-75 5.314576649396007E-4 76-77 0.06039759205103914 78-79 0.1436650074901243 80-81 0.0010114839429495627 82-83 8.571897821606463E-4 84-85 0.0 86-87 0.10696014101800545 88-89 0.35479085083629147 90-91 0.3922329005210686 92-93 3.6001970850747144E-4 94-95 4.114510954371102E-4 96-97 1.2000656950249048E-4 98-99 0.0 100-101 9.771963516631368E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2916507.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.94760014501727 #Duplication Level Percentage of deduplicated Percentage of total 1 64.8500652293046 23.312042142413294 2 15.688794419306237 11.27949017085198 3 6.888292372263033 7.428527396402518 4 3.7490478990385956 5.390770991966261 5 2.3050340234857676 4.143022069846337 6 1.4603531456878118 3.149771457102217 7 1.0088536694730026 2.538610782053424 8 0.7070907216890903 2.0334571623624886 9 0.5177094547717763 1.6749371224287646 >10 2.503737448389959 15.920945234677117 >50 0.1841622072956738 4.592750968094944 >100 0.12328658777457441 8.317354070899698 >500 0.007359910260756249 1.7943418917959424 >1k 0.0049703290072555025 3.638505616207597 >5k 5.734995008371734E-4 1.2821390925716893 >10k+ 6.690827509767024E-4 3.5033338303258597 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19705 0.6756369862990214 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17721 0.6076104051867525 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 17050 0.5846034314335607 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14206 0.4870895218149656 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12389 0.4247889684475299 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10707 0.36711723990376155 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10182 0.349116254478388 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7060 0.2420703944821665 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6894 0.23637865432861982 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6408 0.21971488496341685 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6382 0.2188234075899698 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5402 0.18522156812927243 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5169 0.17723255935953522 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4956 0.1699293024155265 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4491 0.1539855724673385 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4164 0.14277353011667723 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3934 0.13488738412079931 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3693 0.12662407462077066 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3568 0.12233812570996744 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3233 0.11085178262901477 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3138 0.10759446145680433 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3016 0.10341137531986036 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.3715036514570341E-4 0.0 0.0 0.0 0.0 3 1.3715036514570341E-4 0.0 0.0 0.0 0.0 4 1.3715036514570341E-4 0.0 0.0 0.0 0.0 5 1.3715036514570341E-4 0.0 0.0 0.0 0.0 6 1.7143795643212925E-4 0.0 0.0 0.0 0.0 7 2.057255477185551E-4 0.0 0.0 0.0 0.0 8 2.057255477185551E-4 0.0 0.0 0.0 0.0 9 2.057255477185551E-4 0.0 0.0 0.0 0.0 10-11 2.7430073029140683E-4 0.0 0.0 1.7143795643212927E-5 0.0 12-13 3.6001970850747144E-4 0.0 0.0 6.857518257285171E-5 5.143138692963878E-5 14-15 4.4573868672353605E-4 0.0 0.0 6.857518257285171E-5 8.571897821606464E-5 16-17 4.4573868672353605E-4 0.0 0.0 3.257321172210456E-4 1.0286277385927755E-4 18-19 5.143138692963878E-4 0.0 0.0 3.9430729979389727E-4 1.3715036514570341E-4 20-21 5.828890518692394E-4 0.0 0.0 6.68608030085304E-4 1.7143795643212925E-4 22-23 6.171766431556653E-4 0.0 0.0 0.0017143795643212926 1.7143795643212925E-4 24-25 7.886145995877945E-4 0.0 0.0 0.003034451828848688 1.7143795643212925E-4 26-27 7.886145995877945E-4 0.0 0.0 0.005486014605828137 1.7143795643212925E-4 28-29 0.0010457715342359884 0.0 0.0 0.010954885416013058 1.7143795643212925E-4 30-31 0.001131490512452053 0.0 0.0 0.025629974486603323 1.7143795643212925E-4 32-33 0.001131490512452053 0.0 0.0 0.05206570736843766 1.7143795643212925E-4 34-35 0.0011486343080952658 0.0 0.0 0.09288508479492763 1.7143795643212925E-4 36-37 0.0012343532863113306 0.0 0.0 0.15551137027958445 1.7143795643212925E-4 38-39 0.001337216060170608 0.0 0.0 0.23912166163153387 1.7143795643212925E-4 40-41 0.001337216060170608 0.0 0.0 0.3373041792802143 1.7143795643212925E-4 42-43 0.0014400788340298855 0.0 0.0 0.43490380787702554 1.7143795643212925E-4 44-45 0.0016458043817484409 0.0 0.0 0.545652727732181 1.7143795643212925E-4 46-47 0.001800098542537357 0.0 0.0 0.6900892060262499 1.7143795643212925E-4 48-49 0.0018858175207534219 0.0 0.0 0.8485835967477534 1.7143795643212925E-4 50-51 0.0018858175207534219 0.0 0.0 1.0191129320107923 1.7143795643212925E-4 52-53 0.0018858175207534219 0.0 0.0 1.1820818533951742 2.057255477185551E-4 54-55 0.0018858175207534219 0.0 0.0 1.3567942747951574 2.057255477185551E-4 56-57 0.0019886802946126993 0.0 0.0 1.5674743794546009 2.057255477185551E-4 58-59 0.0019886802946126993 0.0 0.0 1.8173452009544293 2.057255477185551E-4 60-61 0.0019886802946126993 0.0 0.0 2.1138128590125107 2.057255477185551E-4 62-63 0.0019886802946126993 0.0 0.0 2.38775356959541 2.057255477185551E-4 64-65 0.0019886802946126993 0.0 0.0 2.650379375053789 2.057255477185551E-4 66-67 0.0020229678858991254 0.0 0.0 2.955710375459411 2.057255477185551E-4 68-69 0.0020229678858991254 0.0 0.0 3.301517877378659 2.057255477185551E-4 70-71 0.0021086868641151902 0.0 0.0 3.6965795041808573 2.4001313900498097E-4 72-73 0.0022286934336176803 0.0 0.0 4.097487165297392 2.7430073029140683E-4 74-75 0.002262981024904106 0.0 0.0 4.4961147015933784 2.7430073029140683E-4 76-77 0.002262981024904106 0.0 0.0 4.903828449580269 2.7430073029140683E-4 78-79 0.002280124820547319 0.0 0.0 5.375214254586051 2.7430073029140683E-4 80-81 0.0026401445290547905 0.0 0.0 5.891362510016263 2.7430073029140683E-4 82-83 0.002674432120341216 0.0 0.0 6.411985296109353 3.428759128642585E-4 84-85 0.002725863507270855 0.0 0.0 6.973496034811506 3.428759128642585E-4 86-87 0.002828726281130133 0.0 0.0 7.55288775236953 3.428759128642585E-4 88-89 0.002845870076773346 0.0 0.0 8.136736856794789 3.428759128642585E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2250 0.0 23.20223 2 GAGTACT 14505 0.0 22.635433 12-13 GTACATG 22985 0.0 21.75612 1 GTACTTT 15505 0.0 21.022552 14-15 TACATGG 23490 0.0 21.012121 2 ACATGGG 23360 0.0 20.397667 3 AGTACTT 15100 0.0 20.376682 12-13 CCGGTAT 120 0.004719136 19.824944 1 ATGGGAG 4875 0.0 18.983644 5 CATGGGG 6625 0.0 18.912006 4 ACTTTTT 17335 0.0 18.898901 16-17 CATGGGA 16305 0.0 18.075502 4 TACTTTT 17200 0.0 17.640566 14-15 ATGGGAT 5490 0.0 17.289293 5 GTGTAGC 1180 0.0 16.531988 1 CGCGGAA 335 0.0 16.382536 82-83 GTATAGG 845 0.0 16.329185 1 TAAGGTG 930 0.0 15.819703 5 ATGGGCG 420 8.731149E-10 15.819702 5 GTCGGGA 920 0.0 15.475797 2 >>END_MODULE