FastQCFastQC Report
Thu 26 May 2016
SRR938711_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938711_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4066303
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125940.30971622134405624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97410.23955420931494772No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT77940.19167287828772228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61100.15025933876545844No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45060.11081318829413352No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA238750.023.8054471
GTACATG146600.023.0868551
TACATGG142450.023.0470122
CGTATAC1703.2255084E-622.2938423
ACATGGG140550.022.2462563
GAGTACT125250.021.88817412-13
GGTATCA177700.021.0736731
ATCAACG271200.020.6651693
TATCAAC273750.020.5765042
TCAACGC272300.020.564294
CAACGCA273500.020.4394195
AACGCAG279550.020.0012136
GTACTTT138400.019.89437714-15
AGTACTT134350.019.29546212-13
AGAGTAC206600.019.12490810-11
ACGCAGA305000.018.1473277
GTATAGG12300.018.1131211
GTGTAGC25650.018.1108681
CGCAGAG306350.018.0519038
CATGGGG70650.017.9707624
Produced by FastQC (version 0.11.5)