FastQCFastQC Report
Thu 26 May 2016
SRR938711_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938711_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4066303
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123100.30273199021322317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103340.25413748065503233No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT87460.2150848079938952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66520.16358839958556948No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA240450.021.6151241
GTACATG143350.021.4015851
TACATGG141700.021.1645412
GGTATCA184850.021.0037061
ACATGGG141300.019.9818843
GAGTACT137050.019.89344612-13
AGTACTT144700.018.9237112-13
AGAGTAC211450.018.90833310-11
GTACTTT146350.018.61307514-15
ATCAACG278300.018.4661733
TCAACGC278950.018.4231434
CAACGCA280350.018.3480685
TATCAAC282100.018.3015332
TATACCG2905.2823452E-817.9993295
GTGTAGC25700.017.9802761
AACGCAG285950.017.9721476
GAGTACA122300.017.9179861
TACCTGG31400.017.6814482
CATGGGG75150.017.4277674
GGACCGT4901.8189894E-1216.463216
Produced by FastQC (version 0.11.5)