##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938711_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4066303 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00758108778416 33.0 31.0 34.0 30.0 34.0 2 32.23083597065934 34.0 31.0 34.0 30.0 34.0 3 32.32382928670096 34.0 31.0 34.0 30.0 34.0 4 35.94715593009178 37.0 35.0 37.0 35.0 37.0 5 35.85720936191917 37.0 35.0 37.0 35.0 37.0 6 35.88438048025442 37.0 35.0 37.0 35.0 37.0 7 35.82181406550372 37.0 35.0 37.0 33.0 37.0 8 35.78642417940817 37.0 35.0 37.0 33.0 37.0 9 37.44327267299067 39.0 37.0 39.0 34.0 39.0 10-11 37.37079172407959 39.0 37.0 39.0 34.0 39.0 12-13 37.29962732732903 39.0 37.0 39.0 34.0 39.0 14-15 38.534000663501956 40.0 38.0 41.0 34.0 41.0 16-17 38.46394550529068 40.0 38.0 41.0 33.5 41.0 18-19 38.48361041958752 40.0 38.0 41.0 33.5 41.0 20-21 38.560257314813974 40.0 38.0 41.0 34.0 41.0 22-23 38.51922434211125 40.0 38.0 41.0 34.0 41.0 24-25 38.45103021098034 40.0 38.0 41.0 34.0 41.0 26-27 38.29171498041342 40.0 38.0 41.0 33.5 41.0 28-29 38.1391643711745 40.0 38.0 41.0 33.0 41.0 30-31 37.924169940115135 40.0 37.0 41.0 33.0 41.0 32-33 37.70979031813418 40.0 37.0 41.0 32.5 41.0 34-35 37.67346899628483 40.0 37.0 41.0 32.5 41.0 36-37 37.5927414902431 40.0 37.0 41.0 32.0 41.0 38-39 37.492823702513064 40.0 37.0 41.0 32.0 41.0 40-41 37.344523145471456 40.0 36.5 41.0 31.0 41.0 42-43 37.17599684529166 40.0 36.0 41.0 31.0 41.0 44-45 36.940334992252176 40.0 36.0 41.0 31.0 41.0 46-47 36.673955679151305 40.0 36.0 41.0 30.0 41.0 48-49 36.414135395222644 39.0 35.5 41.0 30.0 41.0 50-51 36.186475159376954 39.0 35.0 41.0 29.5 41.0 52-53 35.981029451076324 39.0 35.0 41.0 29.0 41.0 54-55 35.7516558652909 39.0 35.0 40.0 28.0 41.0 56-57 35.49776541492358 38.0 35.0 40.0 28.0 41.0 58-59 35.198801589551984 38.0 34.0 40.0 27.5 41.0 60-61 34.85592969830335 38.0 34.0 40.0 26.5 41.0 62-63 34.49448799068835 37.0 34.0 40.0 26.0 41.0 64-65 34.1401074883008 37.0 33.5 39.5 26.0 41.0 66-67 33.754378731737404 36.0 33.0 39.0 25.5 41.0 68-69 33.311486871489905 36.0 32.5 39.0 24.5 40.0 70-71 32.83590069407026 35.0 32.0 38.5 23.5 40.0 72-73 32.479270482302965 35.0 32.0 37.5 22.5 40.0 74-75 32.059397934684156 35.0 31.5 37.0 22.0 39.0 76-77 30.012074727338323 33.0 29.0 35.0 19.5 37.5 78-79 31.079711718482365 34.0 30.5 36.0 20.0 38.5 80-81 31.13226990216912 34.5 31.0 36.0 20.0 37.5 82-83 30.820241014995688 34.0 31.0 35.0 19.0 37.0 84-85 30.425370416321655 34.0 31.0 35.0 17.5 36.5 86-87 29.893248363439714 34.0 30.0 35.0 10.0 36.0 88-89 29.37102215452218 34.0 29.5 35.0 6.0 36.0 90-91 28.951855899572656 34.0 29.0 35.0 2.0 35.0 92-93 28.664790351333878 34.0 29.0 35.0 2.0 35.0 94-95 28.274500203255883 34.0 29.0 35.0 2.0 35.0 96-97 27.75612306805469 33.5 28.0 35.0 2.0 35.0 98-99 27.016078241095165 33.0 26.5 35.0 2.0 35.0 100-101 23.783456127101203 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03746675497109919 1101 2 0.03272988545491984 1101 3 0.07219712001665357 1101 4 0.05716469880149333 1101 5 0.06895734785120311 1101 6 0.14472703441954593 1101 7 0.13498838654335543 1101 8 0.06759268638364802 1101 9 0.16667946512781384 1101 10-11 0.1469201131506921 1101 12-13 0.19890366358440303 1101 14-15 0.18609649006187112 1101 16-17 0.20805266130352607 1101 18-19 0.2201616548385843 1101 20-21 0.24500612068514727 1101 22-23 0.21829652822444956 1101 24-25 0.1592518177160258 1101 26-27 0.24620906148575017 1101 28-29 0.1781202761282259 1101 30-31 0.09659623376023063 1101 32-33 0.20198316542927586 1101 34-35 0.18164208051945963 1101 36-37 0.3363719891533492 1101 38-39 0.2791353499244167 1101 40-41 0.26703504796358146 1101 42-43 0.19465330335605557 1101 44-45 0.18707171490952845 1101 46-47 0.24995175027505212 1101 48-49 0.07485685314206592 1101 50-51 0.10563038434352734 1101 52-53 0.1604576329186358 1101 54-55 0.19729268175672843 1101 56-57 0.03523731378522399 1101 58-59 0.04972506548207889 1101 60-61 0.05490099696427819 1101 62-63 -0.008664112670622615 1101 64-65 -0.04191259840051487 1101 66-67 -0.1397200670474703 1101 68-69 -0.09448165673715891 1101 70-71 -0.1261851383532715 1101 72-73 -0.09250810217438499 1101 74-75 0.01893019378220373 1101 76-77 -0.06906741709570596 1101 78-79 0.08609950637531227 1101 80-81 0.10062683208098377 1101 82-83 0.03206511588807004 1101 84-85 0.14345998928075687 1101 86-87 0.2238691509380999 1101 88-89 0.37653593244385064 1101 90-91 0.45252119247274436 1101 92-93 0.3182272494181291 1101 94-95 0.20335821135443766 1101 96-97 0.27504429586242196 1101 98-99 0.37219344137234245 1101 100-101 0.9560513973786087 1102 1 0.05934319555328216 1102 2 0.0625648047970273 1102 3 0.11522530073147408 1102 4 0.08313242169048607 1102 5 0.11567236265616287 1102 6 0.11828844431575192 1102 7 0.08946568840509173 1102 8 0.12959916082400724 1102 9 0.18769116361671934 1102 10-11 0.17664637579705555 1102 12-13 0.19674483850307922 1102 14-15 0.23346218534715746 1102 16-17 0.19895967210226928 1102 18-19 0.24255856469901005 1102 20-21 0.22317552409657537 1102 22-23 0.22469125254780664 1102 24-25 0.12077197269329076 1102 26-27 0.1784511142368288 1102 28-29 0.1910052677315548 1102 30-31 0.1481890750281778 1102 32-33 0.24968258276582134 1102 34-35 0.21071433580093668 1102 36-37 0.18337822586680375 1102 38-39 0.1787607879426858 1102 40-41 0.18628849119220092 1102 42-43 0.22847786788302216 1102 44-45 0.12430971467541951 1102 46-47 0.08265787435247063 1102 48-49 0.15058265661375714 1102 50-51 0.143354671272661 1102 52-53 0.15902351957406324 1102 54-55 0.11007733710570022 1102 56-57 0.02880008285912794 1102 58-59 0.026834355513287278 1102 60-61 -0.008734940314063522 1102 62-63 -0.057443766194005264 1102 64-65 -0.03421541492160429 1102 66-67 0.00970462740240663 1102 68-69 0.06123850459671587 1102 70-71 0.07189853738000807 1102 72-73 0.15127860176028918 1102 74-75 0.22416218164159574 1102 76-77 0.016517721820783038 1102 78-79 0.10931289184663484 1102 80-81 0.1607327833412846 1102 82-83 0.2386690398805804 1102 84-85 0.2319463976330347 1102 86-87 0.12473051042070082 1102 88-89 0.34076136136648216 1102 90-91 0.3965983301995628 1102 92-93 0.4496651586410785 1102 94-95 0.47334991287694805 1102 96-97 0.4960288883171238 1102 98-99 0.5496521770218408 1102 100-101 0.9603048354392527 1103 1 0.001971063875970458 1103 2 0.05420009798552172 1103 3 9.620250202075908E-4 1103 4 0.01981874210549961 1103 5 0.054740845429179785 1103 6 0.044016112153144604 1103 7 0.06442417699653902 1103 8 0.12230485521855883 1103 9 0.06149545508894505 1103 10-11 0.033244243758538516 1103 12-13 0.054498501101761576 1103 14-15 0.08721368045397071 1103 16-17 -0.014510785587916075 1103 18-19 0.05675503336304644 1103 20-21 0.06719527968402872 1103 22-23 0.05212120090307337 1103 24-25 0.10387148349992259 1103 26-27 0.12958518840361677 1103 28-29 0.1969372561942606 1103 30-31 0.07661937118042061 1103 32-33 0.12104607206331508 1103 34-35 -0.09490060629160268 1103 36-37 -0.15472189154755256 1103 38-39 -0.03696075864206705 1103 40-41 -0.004566084051184305 1103 42-43 0.10086842009751251 1103 44-45 0.03817869856865741 1103 46-47 -0.0013553397517043209 1103 48-49 0.06836084400055853 1103 50-51 0.1217852593408395 1103 52-53 -0.013429476987937505 1103 54-55 0.0035070407648376545 1103 56-57 -0.03841734684478837 1103 58-59 -0.027192964867147396 1103 60-61 -0.03683480045746279 1103 62-63 -0.04334275083338923 1103 64-65 0.029774675590942934 1103 66-67 -0.09708380392411442 1103 68-69 -0.20442902470827562 1103 70-71 -0.24099229715889692 1103 72-73 -0.19780178925822156 1103 74-75 -0.046556263256128716 1103 76-77 -0.09202916619814872 1103 78-79 -0.09264292568191124 1103 80-81 -0.039713090985117105 1103 82-83 -0.07852742640964294 1103 84-85 -0.1666362959606431 1103 86-87 -0.08615796684889432 1103 88-89 0.05644932157917282 1103 90-91 0.17999099517970407 1103 92-93 0.17400313339503626 1103 94-95 0.11306466545509508 1103 96-97 0.11827250416370916 1103 98-99 0.4283233487319933 1103 100-101 0.8039342256384074 1104 1 -0.009591443303804681 1104 2 0.015073666881342263 1104 3 -0.02654746714988221 1104 4 -0.04537081552953026 1104 5 -0.015256998187254567 1104 6 -0.017917856938865384 1104 7 -0.05703072556713806 1104 8 -0.061039268471652974 1104 9 -0.0667674675000498 1104 10-11 0.03637856341635626 1104 12-13 -0.04724381504912856 1104 14-15 0.018501470569681544 1104 16-17 0.027972818903108987 1104 18-19 0.021237364277610027 1104 20-21 5.557487237197734E-4 1104 22-23 0.015481383226664036 1104 24-25 -0.04410234824430859 1104 26-27 -0.03705598183858427 1104 28-29 0.011992269292377955 1104 30-31 -0.050294466707001106 1104 32-33 -0.04389008676753292 1104 34-35 0.017206591858531795 1104 36-37 -0.019892220848909403 1104 38-39 0.11167476702722468 1104 40-41 0.1652038360879402 1104 42-43 0.2134007239196123 1104 44-45 0.22568700969625866 1104 46-47 0.294593526298776 1104 48-49 0.21339355716845176 1104 50-51 0.21307272286603052 1104 52-53 0.13663861650378806 1104 54-55 0.12293385413816083 1104 56-57 0.2192185896576575 1104 58-59 0.1814788553895781 1104 60-61 0.25343322593131035 1104 62-63 0.04529103808318524 1104 64-65 0.22593187601644615 1104 66-67 0.15860920376639598 1104 68-69 0.300830760016666 1104 70-71 0.20764453867005983 1104 72-73 0.10747762887391588 1104 74-75 0.16738436094064468 1104 76-77 0.08347605700337724 1104 78-79 0.16076875857517692 1104 80-81 0.1436460963766386 1104 82-83 0.13165895741106226 1104 84-85 0.2896709867359277 1104 86-87 0.45564631668965916 1104 88-89 0.49704506826923023 1104 90-91 0.558776193616044 1104 92-93 0.5241725142547189 1104 94-95 0.5216068495267052 1104 96-97 0.5110686893331966 1104 98-99 0.5575278140852618 1104 100-101 1.0978576924398809 1105 1 -0.03575333984544571 1105 2 -0.0283774283795708 1105 3 -0.0163513447181316 1105 4 -0.07974202016588094 1105 5 0.012017651062045331 1105 6 -0.007905940401712996 1105 7 -0.027355932531627047 1105 8 -0.04170448348404676 1105 9 0.013674221580409096 1105 10-11 0.02774955787781863 1105 12-13 0.015070647213477173 1105 14-15 0.09450531969629594 1105 16-17 0.002810728588045208 1105 18-19 -0.07215657745456383 1105 20-21 0.060987987078817696 1105 22-23 0.02109149334078353 1105 24-25 -0.025858043514702445 1105 26-27 -0.016933149744716047 1105 28-29 0.0408447212405747 1105 30-31 -0.030457728422454977 1105 32-33 -0.08019840431911263 1105 34-35 0.01807161404182267 1105 36-37 -0.052961310360487346 1105 38-39 0.008304321020958128 1105 40-41 0.0034538825743055668 1105 42-43 -0.018161430907888132 1105 44-45 0.055311312299885174 1105 46-47 0.1732703365286241 1105 48-49 0.14047160001840808 1105 50-51 0.17768586967489597 1105 52-53 0.16955024569838884 1105 54-55 0.2130703025578029 1105 56-57 0.0993864114785552 1105 58-59 0.07480304738890453 1105 60-61 0.05071380021136207 1105 62-63 2.0075886742887405E-4 1105 64-65 0.048208599989472134 1105 66-67 -0.008535994402180336 1105 68-69 0.07930503073993833 1105 70-71 0.057873839285939255 1105 72-73 -0.1158576981076962 1105 74-75 -0.0580299765154777 1105 76-77 -0.06680255984830197 1105 78-79 -0.023498796852312154 1105 80-81 0.09797244672233418 1105 82-83 0.03227527989227852 1105 84-85 0.1354599623268733 1105 86-87 0.18896075727002426 1105 88-89 0.4142667600323193 1105 90-91 0.48918718274951445 1105 92-93 0.5265848348892739 1105 94-95 0.5750353188366084 1105 96-97 0.5999019043460798 1105 98-99 0.7489196023790754 1105 100-101 0.9562579378655016 1106 1 -0.05954027911719706 1106 2 -0.11162454669673139 1106 3 -0.1199905083620223 1106 4 -0.04587855409726416 1106 5 -0.08097677011566873 1106 6 -0.12254607830618625 1106 7 -0.0861698388715908 1106 8 -0.095646407071456 1106 9 -0.054135402808888955 1106 10-11 -0.19357220669820663 1106 12-13 -0.10735649893474886 1106 14-15 0.01491300733169254 1106 16-17 -0.1402644813130678 1106 18-19 -0.25777039994036954 1106 20-21 -0.1911179769237279 1106 22-23 -0.20509329257033215 1106 24-25 -0.12474944819278733 1106 26-27 -0.10249784512203064 1106 28-29 -0.1788864226392377 1106 30-31 -0.09796408608460894 1106 32-33 -0.11089258785240474 1106 34-35 -0.18792074984165907 1106 36-37 -0.199450542197674 1106 38-39 -0.17087291702816287 1106 40-41 -0.13209608517695415 1106 42-43 -0.08247536085655582 1106 44-45 -0.12920084296674617 1106 46-47 -0.23934292651752997 1106 48-49 -0.12498721590271344 1106 50-51 -0.06317178494540343 1106 52-53 -0.08846500057743611 1106 54-55 -0.08442363396174102 1106 56-57 -0.2113842667140986 1106 58-59 -0.15179699206606045 1106 60-61 -0.12846356144351745 1106 62-63 -0.12683360649982234 1106 64-65 -0.05143163927171912 1106 66-67 -0.09382047552622197 1106 68-69 -0.16574684536061568 1106 70-71 -0.30546883987874907 1106 72-73 -0.19165092569877373 1106 74-75 -0.01862053639764838 1106 76-77 -0.09811662625771689 1106 78-79 -0.024974989518089075 1106 80-81 -0.09251060828791324 1106 82-83 0.05732407944551454 1106 84-85 -0.05173366535132473 1106 86-87 0.0517413083489906 1106 88-89 0.2415974578436817 1106 90-91 0.18275036964216085 1106 92-93 0.2922086644972559 1106 94-95 0.18245752940692483 1106 96-97 0.26053997828160647 1106 98-99 0.3661235709149828 1106 100-101 0.8106076801104152 1107 1 -0.02055154795420222 1107 2 -0.011364468354862822 1107 3 -0.07207211467638786 1107 4 -0.06621713348666702 1107 5 -0.04522028376917575 1107 6 -0.12621729716015295 1107 7 -0.10793640019264927 1107 8 -0.05368893796188701 1107 9 -0.10340261616251212 1107 10-11 -0.05276595771604775 1107 12-13 -0.08025921934931546 1107 14-15 -0.0946487594377956 1107 16-17 -0.0368521775503865 1107 18-19 -0.011755145439515502 1107 20-21 -0.023505842628281925 1107 22-23 -0.15646142832045484 1107 24-25 -0.15698042812732638 1107 26-27 -0.14045908928587636 1107 28-29 -0.10235509507644736 1107 30-31 -0.15579778288273616 1107 32-33 -0.0955257108618639 1107 34-35 -0.049432369490553185 1107 36-37 -0.08746927543003125 1107 38-39 -0.10286224695909141 1107 40-41 -0.12978948170949423 1107 42-43 -0.16134375068509144 1107 44-45 -0.2056609673488481 1107 46-47 -0.23680110153653544 1107 48-49 -0.3918720430742795 1107 50-51 -0.3140333556765498 1107 52-53 -0.33419026895158765 1107 54-55 -0.3838047540373637 1107 56-57 -0.31724408032462037 1107 58-59 -0.2561981506636428 1107 60-61 -0.3037443027892337 1107 62-63 -0.31537794532655283 1107 64-65 -0.16674114964118303 1107 66-67 -0.1789313271848343 1107 68-69 -0.2989400172021135 1107 70-71 -0.36104071835702456 1107 72-73 -0.26674245515290096 1107 74-75 -0.12347854635752498 1107 76-77 -0.28880808077400744 1107 78-79 -0.29434179548911743 1107 80-81 -0.33570311279092024 1107 82-83 -0.20512928338948555 1107 84-85 -0.2887100954454702 1107 86-87 -0.17237446814526436 1107 88-89 -0.04285585111444945 1107 90-91 0.11239210682834155 1107 92-93 0.1484421570530614 1107 94-95 0.1301854867266492 1107 96-97 0.02311468651999249 1107 98-99 0.015441477504737122 1107 100-101 0.5430685857071182 1108 1 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1388.0 9 2618.0 10 4228.0 11 6512.0 12 9086.0 13 12385.0 14 15763.0 15 20005.0 16 24638.0 17 31905.0 18 30619.0 19 22988.0 20 21714.0 21 22717.0 22 24302.0 23 27128.0 24 31850.0 25 37841.0 26 46106.0 27 57267.0 28 69511.0 29 85379.0 30 103164.0 31 125432.0 32 154553.0 33 194301.0 34 256925.0 35 357828.0 36 539163.0 37 856163.0 38 781142.0 39 90660.0 40 9.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.400284603114685 19.654155864643666 14.775760248045145 23.169799284196504 2 15.90936533750682 21.071031843913254 39.00803752204398 24.011565296535945 3 19.768153037292105 24.40479718309236 29.78240923020247 26.044640549413067 4 13.215960542045194 17.15575548600289 37.09396471438552 32.534319257566395 5 13.47339339936055 37.6521867652263 34.503183850293496 14.371235985119654 6 31.8205751022489 36.633866192460324 17.238041533058407 14.30751717223237 7 28.15478826836072 32.673364478741505 20.860939285636118 18.310907967261663 8 26.037090693930086 35.14516748997799 19.7091067734008 19.108635042691112 9 25.774370478540337 17.0467129478546 20.47820833813909 36.700708235465974 10-11 24.859399311856496 26.820468125469255 27.360393458136294 20.959739104537956 12-13 25.83866721196133 23.95882697378921 27.797399751076103 22.405106063173356 14-15 22.470266841289245 25.692205936438185 26.329481627432944 25.508045594839622 16-17 21.903000936128677 28.713499988748747 27.021115093657748 22.362383981464827 18-19 21.744471261083177 27.45693994881831 29.221505654529008 21.577083135569502 20-21 22.587928468184547 26.475969262001538 29.294561828589977 21.64154044122394 22-23 22.044618908133508 26.564510809942103 29.145442432597868 22.245427849326525 24-25 22.108227055386674 26.630332269877577 29.358842663716896 21.902598011018853 26-27 21.940745850494213 26.732242291667053 29.337167287932083 21.98984456990665 28-29 21.71281874469266 27.290342111741307 29.166518087806047 21.830321055759985 30-31 22.076588980211262 26.774394333132577 29.074665611490342 22.074351075165822 32-33 21.819229899985316 26.879145503913506 29.065873841668953 22.23575075443222 34-35 22.42539454868063 26.912672176003653 28.918706844172533 21.743226431143185 36-37 22.359902031499715 26.624753956526632 29.081960378468818 21.933383633504832 38-39 21.99114693275469 27.005015590326437 29.061585939868255 21.942251537050623 40-41 22.270710030975387 26.922182539199436 28.9137365851523 21.893370844672877 42-43 22.000126655669348 26.998992288145335 28.60068234204778 22.400198714137538 44-45 22.172976430593046 26.96228405490154 28.668547444933257 22.196192069572156 46-47 22.4416130503065 27.167533890081092 28.23775021309644 22.153102846515964 48-49 22.32166011300441 27.237011323434825 28.199421513628543 22.24190704993223 50-51 22.23385719397435 27.4064533105771 28.232268520283256 22.127420975165293 52-53 22.47414912267974 27.40432771488008 27.8976136308583 22.22390953158188 54-55 22.09671782943868 27.26410206027441 28.11116141615615 22.52801869413076 56-57 22.247456719285307 27.46909170320067 27.88044816138886 22.403003416125163 58-59 22.45247598761179 27.226684592932642 27.9278894883575 22.392949931098066 60-61 22.17745837562734 27.32753728686741 28.30802865766048 22.18697567984477 62-63 22.04547005541275 27.291292273508923 28.326065264450932 22.3371724066274 64-65 21.898958341274618 27.441167074858907 28.093270471974176 22.566604111892303 66-67 21.92847789740605 27.399226339430143 27.84779650358739 22.824499259576413 68-69 22.05295295847951 27.2346434489893 28.0713932020492 22.641010390481988 70-71 22.08883346758967 27.16669865783424 27.873644028437667 22.87082384613843 72-73 22.01465311365139 27.122019682251914 28.0303263185257 22.833000885570996 74-75 21.85281077184853 27.621662724100982 27.66039569604134 22.865130808009145 76-77 21.937797011332126 27.19302521080804 27.87643590093899 22.992741876920842 78-79 21.963612764475624 27.31171889212899 27.856743880077527 22.86792446331786 80-81 22.146977769973798 27.29491070756673 27.655693059689124 22.90241846277035 82-83 22.077252974015956 27.25934343800745 27.86089723269515 22.802506355281444 84-85 21.9366338416001 27.33087032619064 27.773397604654647 22.959098227554612 86-87 21.906930114399945 27.28956595702339 27.744200357348202 23.059303571228458 88-89 21.88081833304465 27.23250040341265 27.558820525043416 23.327860738499282 90-91 21.87328212985933 27.189286384566646 27.63063951565588 23.306791969918145 92-93 22.01917810846855 27.153153613983022 27.715138296383717 23.11252998116471 94-95 21.742138188457226 26.964586004679436 27.83680356254897 23.456472244314366 96-97 21.644214035776738 26.8081080559324 28.029611183528818 23.518066724762047 98-99 21.603059001260238 26.966332615603715 27.671212697457108 23.75939568567894 100-101 21.6445634888284 26.48522767976208 28.138278060940607 23.73193077046891 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1066.0 1 755.0 2 551.0 3 687.0 4 617.0 5 608.0 6 1054.5 7 2158.0 8 3406.0 9 3950.0 10 3519.0 11 3008.0 12 3057.0 13 3273.0 14 3637.5 15 4006.5 16 4320.0 17 4700.0 18 5155.5 19 5748.0 20 6671.5 21 8017.5 22 9719.0 23 11742.5 24 14322.0 25 18034.5 26 23493.5 27 30104.5 28 36320.0 29 43263.0 30 50812.5 31 59175.5 32 69916.0 33 77052.0 34 82005.5 35 89412.5 36 99017.0 37 107824.0 38 112824.0 39 116746.0 40 121063.5 41 126015.0 42 130059.5 43 133706.0 44 138248.5 45 144158.5 46 147381.0 47 154100.5 48 169303.5 49 169008.5 50 153979.5 51 144906.5 52 140231.5 53 136867.5 54 133199.0 55 124657.5 56 113311.0 57 101527.5 58 91644.5 59 80885.0 60 66516.0 61 53970.0 62 44016.0 63 33672.5 64 24098.5 65 17769.0 66 13034.5 67 9933.5 68 7484.5 69 5359.5 70 3957.5 71 2877.5 72 2134.5 73 1772.5 74 1394.5 75 1013.5 76 744.5 77 451.5 78 262.5 79 153.5 80 91.0 81 57.5 82 30.0 83 13.0 84 8.5 85 7.0 86 3.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38732971940359584 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.336274497989943E-4 16-17 0.0021764241376011576 18-19 1.2296181568368123E-4 20-21 1.2296181568368122E-5 22-23 0.0 24-25 0.0 26-27 1.3525799725204934E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007291635670042296 36-37 0.0174728740086511 38-39 0.011816630487201765 40-41 0.008176960742964801 42-43 0.003947074283446168 44-45 0.0019305005062337953 46-47 0.0016968730564348008 48-49 3.688854470510436E-5 50-51 4.4266253646125234E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.05773991756148E-4 60-61 6.271052599867743E-4 62-63 1.2296181568368122E-5 64-65 0.0 66-67 0.001217321975268444 68-69 8.238441650806641E-4 70-71 0.0018813157799603226 72-73 0.0 74-75 0.0 76-77 0.09733657329520207 78-79 0.035499076187878766 80-81 2.4592363136736245E-5 82-83 0.0 84-85 0.0 86-87 0.150443781488984 88-89 0.32907041113266766 90-91 0.2828121760724668 92-93 0.0 94-95 2.4592363136736245E-5 96-97 3.688854470510436E-5 98-99 2.090350866622581E-4 100-101 7.99251801943928E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4066303.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.96392833935812 #Duplication Level Percentage of deduplicated Percentage of total 1 71.07424649949475 36.932970518736205 2 15.529868872513386 16.139859864218277 3 5.787956351021614 9.022948471674598 4 2.707747276836039 5.628207418184003 5 1.4422848291042158 3.7473392752257424 6 0.8646144869497447 2.6957259144616454 7 0.5430212309026753 1.9752261430563665 8 0.37956530413946216 1.5778963411527749 9 0.2763798830805978 1.2925605994956326 >10 1.2772828445252198 11.695162310925927 >50 0.07754981615920055 2.738608717972182 >100 0.035081059728887996 3.225988790833856 >500 0.002650393103750372 0.9850030964658516 >1k 0.0015618386630315835 1.406908283279174 >5k 9.46568886685769E-5 0.37869408359144485 >10k+ 9.46568886685769E-5 0.556900170726372 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12310 0.30273199021322317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10334 0.25413748065503233 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8746 0.2150848079938952 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6652 0.16358839958556948 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.4592363136736245E-5 2 0.0 0.0 0.0 0.0 2.4592363136736245E-5 3 0.0 0.0 0.0 0.0 2.4592363136736245E-5 4 0.0 0.0 0.0 0.0 2.4592363136736245E-5 5 2.4592363136736245E-5 0.0 0.0 0.0 2.4592363136736245E-5 6 2.4592363136736245E-5 0.0 0.0 0.0 2.4592363136736245E-5 7 4.918472627347249E-5 0.0 0.0 0.0 2.4592363136736245E-5 8 4.918472627347249E-5 0.0 0.0 0.0 7.377708941020874E-5 9 4.918472627347249E-5 0.0 0.0 0.0 7.377708941020874E-5 10-11 4.918472627347249E-5 0.0 0.0 0.0 7.377708941020874E-5 12-13 6.148090784184062E-5 0.0 0.0 2.4592363136736245E-5 7.377708941020874E-5 14-15 9.836945254694498E-5 0.0 0.0 7.377708941020874E-5 9.836945254694499E-5 16-17 9.836945254694498E-5 0.0 0.0 9.836945254694498E-5 1.2296181568368123E-4 18-19 9.836945254694498E-5 0.0 0.0 1.2296181568368123E-4 1.2296181568368123E-4 20-21 1.2296181568368123E-4 0.0 0.0 1.9673890509388999E-4 1.2296181568368123E-4 22-23 1.4755417882041748E-4 0.0 0.0 4.180701733245162E-4 1.9673890509388999E-4 24-25 1.4755417882041748E-4 0.0 0.0 7.623632572388236E-4 2.9510835764083495E-4 26-27 1.4755417882041748E-4 0.0 0.0 0.0013525799725204934 3.1970072077757116E-4 28-29 2.213312682306262E-4 0.0 0.0 0.0037872239230573815 3.1970072077757116E-4 30-31 2.4592363136736247E-4 0.0 0.0 0.009209839994707723 3.3199690234593927E-4 32-33 2.582198129357306E-4 0.0 0.0 0.022834009172459604 3.6888544705104365E-4 34-35 3.0740453920920306E-4 0.0 0.0 0.04657793578097845 3.6888544705104365E-4 36-37 3.1970072077757116E-4 0.0 0.0 0.08795458675853718 3.6888544705104365E-4 38-39 3.6888544705104365E-4 0.0 0.0 0.14744351318630217 4.180701733245162E-4 40-41 3.6888544705104365E-4 0.0 0.0 0.21283460676688382 4.426625364612524E-4 42-43 4.05773991756148E-4 0.0 0.0 0.27732807909297463 4.549587180296205E-4 44-45 4.426625364612524E-4 0.0 0.0 0.3640038629684015 4.672548995979886E-4 46-47 4.672548995979886E-4 0.0 0.0 0.46925917719363264 4.672548995979886E-4 48-49 4.672548995979886E-4 0.0 0.0 0.5814987225496968 5.410319890081974E-4 50-51 4.672548995979886E-4 0.0 0.0 0.7038703215180964 5.410319890081974E-4 52-53 4.672548995979886E-4 0.0 0.0 0.8254057801398469 5.533281705765655E-4 54-55 4.672548995979886E-4 0.0 0.0 0.963528787697326 5.656243521449336E-4 56-57 4.918472627347249E-4 0.0 0.0 1.1239078838935515 5.656243521449336E-4 58-59 4.918472627347249E-4 2.4592363136736245E-5 0.0 1.309211340128859 5.902167152816699E-4 60-61 4.918472627347249E-4 2.4592363136736245E-5 0.0 1.5096636920563962 5.902167152816699E-4 62-63 4.918472627347249E-4 2.4592363136736245E-5 0.0 1.7072510336785034 6.148090784184061E-4 64-65 4.918472627347249E-4 2.4592363136736245E-5 0.0 1.916163158525078 6.148090784184061E-4 66-67 4.918472627347249E-4 2.4592363136736245E-5 0.0 2.157389648533324 6.148090784184061E-4 68-69 4.918472627347249E-4 2.4592363136736245E-5 0.0 2.43485298562355 6.148090784184061E-4 70-71 4.918472627347249E-4 2.4592363136736245E-5 0.0 2.7305761523428034 6.271052599867743E-4 72-73 5.164396258714612E-4 2.4592363136736245E-5 0.0 3.034218059992086 7.131785309653511E-4 74-75 5.410319890081974E-4 2.4592363136736245E-5 0.0 3.343147325715767 7.131785309653511E-4 76-77 5.410319890081974E-4 4.918472627347249E-5 0.0 3.683653185707017 7.131785309653511E-4 78-79 5.533281705765655E-4 4.918472627347249E-5 0.0 4.06595376684915 7.131785309653511E-4 80-81 6.394014415551423E-4 4.918472627347249E-5 0.0 4.477961922660461 7.623632572388236E-4 82-83 6.394014415551423E-4 4.918472627347249E-5 0.0 4.899880800815876 8.11547983512296E-4 84-85 6.516976231235104E-4 4.918472627347249E-5 0.0 5.323902326019482 8.11547983512296E-4 86-87 6.639938046918785E-4 4.918472627347249E-5 0.0 5.773696647790389 8.484365282174004E-4 88-89 6.639938046918785E-4 4.918472627347249E-5 0.0 6.259383523559361 9.345097991959772E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 24045 0.0 21.615124 1 GTACATG 14335 0.0 21.401585 1 TACATGG 14170 0.0 21.164541 2 GGTATCA 18485 0.0 21.003706 1 ACATGGG 14130 0.0 19.981884 3 GAGTACT 13705 0.0 19.893446 12-13 AGTACTT 14470 0.0 18.92371 12-13 AGAGTAC 21145 0.0 18.908333 10-11 GTACTTT 14635 0.0 18.613075 14-15 ATCAACG 27830 0.0 18.466173 3 TCAACGC 27895 0.0 18.423143 4 CAACGCA 28035 0.0 18.348068 5 TATCAAC 28210 0.0 18.301533 2 TATACCG 290 5.2823452E-8 17.999329 5 GTGTAGC 2570 0.0 17.980276 1 AACGCAG 28595 0.0 17.972147 6 GAGTACA 12230 0.0 17.917986 1 TACCTGG 3140 0.0 17.681448 2 CATGGGG 7515 0.0 17.427767 4 GGACCGT 490 1.8189894E-12 16.46321 6 >>END_MODULE