FastQCFastQC Report
Thu 26 May 2016
SRR938710_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938710_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4074252
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125620.3083265345393461No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96610.23712328054327517No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT77040.18908992374551206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61600.1511933969720086No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44610.10949249089157961No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA230050.023.6549451
GTACATG148450.023.2431561
TACATGG146950.023.1153852
ACATGGG149400.021.6884163
GGTATCA173650.021.4028031
GAGTACT126200.021.08226212-13
ATCAACG265500.020.2452493
TCAACGC266900.020.1746044
TATCAAC267150.020.1734852
CAACGCA267450.020.1153775
AACGCAG273300.019.648886
GTACTTT139600.019.02507414-15
GTGTAGC27050.018.9755861
AGTACTT134700.018.9590112-13
CATGGGG74900.018.9385664
AGAGTAC206300.018.73950410-11
GAGTACA130100.018.0828381
TAAGGTG17750.017.9074695
ACGCAGA300050.017.7711037
TACCTGG29050.017.6374322
Produced by FastQC (version 0.11.5)