##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938710_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4074252 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.415385204449798 33.0 31.0 34.0 30.0 34.0 2 31.477958162627154 33.0 31.0 34.0 28.0 34.0 3 31.495222681365806 33.0 31.0 34.0 28.0 34.0 4 35.03779417669796 37.0 35.0 37.0 32.0 37.0 5 35.06865063820304 37.0 35.0 37.0 32.0 37.0 6 35.12033595369162 37.0 35.0 37.0 32.0 37.0 7 35.02358641537146 37.0 35.0 37.0 32.0 37.0 8 34.98571467842441 37.0 35.0 37.0 32.0 37.0 9 36.542945306279535 39.0 37.0 39.0 32.0 39.0 10-11 36.42107189246026 39.0 37.0 39.0 32.0 39.0 12-13 36.30339323635357 39.0 37.0 39.0 32.0 39.0 14-15 37.44053018811796 40.0 37.0 41.0 32.0 41.0 16-17 37.31906372016262 40.0 37.0 41.0 32.0 41.0 18-19 37.25603313197122 40.0 37.0 41.0 31.0 41.0 20-21 37.13460470780895 40.0 37.0 41.0 31.0 41.0 22-23 37.159025018580095 40.0 37.0 41.0 31.0 41.0 24-25 37.094445925288866 40.0 37.0 41.0 31.0 41.0 26-27 36.95905248374426 40.0 36.5 41.0 30.0 41.0 28-29 36.784351090703275 40.0 36.0 41.0 30.0 41.0 30-31 36.56068340888095 39.0 36.0 41.0 30.0 41.0 32-33 36.37143259670732 39.0 35.5 41.0 30.0 41.0 34-35 36.07877740503042 39.0 35.0 41.0 28.5 41.0 36-37 35.86902540638134 39.0 35.0 41.0 27.0 41.0 38-39 35.69538617149847 39.0 35.0 41.0 27.0 41.0 40-41 35.588421506573475 39.0 35.0 40.5 27.0 41.0 42-43 35.57696112071615 39.0 35.0 41.0 26.5 41.0 44-45 35.40866482976507 39.0 35.0 41.0 25.5 41.0 46-47 35.12974749720931 39.0 35.0 41.0 24.5 41.0 48-49 34.8520003180952 39.0 34.0 41.0 24.0 41.0 50-51 33.734769351527596 38.0 32.5 39.5 22.0 40.5 52-53 33.81233831387945 38.0 33.0 39.5 22.5 40.5 54-55 34.31250484751557 38.0 34.0 40.0 23.0 41.0 56-57 34.20045802272417 38.0 34.0 40.0 22.0 41.0 58-59 33.988681235230416 38.0 34.0 40.0 21.5 41.0 60-61 33.679457358062294 38.0 33.0 40.0 20.0 41.0 62-63 33.28341067268299 37.0 33.0 40.0 18.5 41.0 64-65 32.901426445885036 36.5 32.0 40.0 17.5 41.0 66-67 32.50895207267494 36.0 32.0 39.0 13.5 41.0 68-69 32.08477961107953 35.5 31.0 39.0 11.0 40.5 70-71 31.65904318142324 35.0 31.0 38.5 8.0 40.0 72-73 31.238352708668977 35.0 31.0 37.5 7.0 40.0 74-75 30.77481866610116 35.0 30.0 37.0 4.0 39.0 76-77 30.312156440004202 35.0 30.0 36.5 2.0 39.0 78-79 29.87209750403264 34.0 29.5 36.0 2.0 39.0 80-81 29.40187646714047 34.0 29.0 35.5 2.0 37.5 82-83 28.97929264071049 34.0 29.0 35.0 2.0 37.0 84-85 28.62161312064153 34.0 29.0 35.0 2.0 36.5 86-87 28.19938678314449 34.0 27.5 35.0 2.0 36.0 88-89 27.734303130979626 34.0 26.5 35.0 2.0 36.0 90-91 27.277321211353645 34.0 26.0 35.0 2.0 35.0 92-93 26.809484293067783 33.0 25.0 35.0 2.0 35.0 94-95 26.282021951514047 33.0 24.5 35.0 2.0 35.0 96-97 25.664724469669522 33.0 21.5 35.0 2.0 35.0 98-99 24.875666379988278 32.0 10.0 35.0 2.0 35.0 100-101 22.358327982657922 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09289651585006098 1101 2 0.18419322358241175 1101 3 0.3125784387155477 1101 4 0.11607926198540497 1101 5 0.060546992371577346 1101 6 0.1629544683917743 1101 7 0.17719004640191116 1101 8 0.21733819375332075 1101 9 0.21704166970744154 1101 10-11 0.19666997121431962 1101 12-13 0.18198459571738823 1101 14-15 0.2239089116820736 1101 16-17 0.31202168307567746 1101 18-19 0.40728979781695784 1101 20-21 0.47967951238846496 1101 22-23 0.28589496462481634 1101 24-25 0.36513275972038883 1101 26-27 0.4347475742222784 1101 28-29 0.46706085523385354 1101 30-31 0.42631875171122147 1101 32-33 0.37940291053975983 1101 34-35 0.41751300880213904 1101 36-37 0.45320800065032785 1101 38-39 0.4932735153617358 1101 40-41 0.5995494260412428 1101 42-43 0.63275990007687 1101 44-45 0.6310332630101954 1101 46-47 0.562916845592504 1101 48-49 0.5923947867828829 1101 50-51 0.6204310038700669 1101 52-53 0.6587173407597433 1101 54-55 0.668898374626913 1101 56-57 0.6794774390291565 1101 58-59 0.6713677527846684 1101 60-61 0.587694421833092 1101 62-63 0.5287951578280001 1101 64-65 0.5428019903414665 1101 66-67 0.6196642430254755 1101 68-69 0.692839614497565 1101 70-71 0.6323187699054067 1101 72-73 0.5148040189066165 1101 74-75 0.39395828628635954 1101 76-77 0.3423959358677777 1101 78-79 0.38287103125873045 1101 80-81 0.3251973134355346 1101 82-83 0.4967181559849152 1101 84-85 0.33987210021701486 1101 86-87 0.27711093162769984 1101 88-89 0.35791857991139864 1101 90-91 0.36940032430043246 1101 92-93 0.2756562252888912 1101 94-95 0.15119599476707535 1101 96-97 0.0909303908972312 1101 98-99 0.1073147220030286 1101 100-101 0.3387358900180111 1102 1 0.11717512045338196 1102 2 0.1855299603816576 1102 3 0.18831894513325764 1102 4 0.31481969962779033 1102 5 0.22969815263574844 1102 6 0.3712964290729772 1102 7 0.26281277658776503 1102 8 0.3154186316756764 1102 9 0.45752690705232624 1102 10-11 0.44942387543158446 1102 12-13 0.4771091855760119 1102 14-15 0.573534512172877 1102 16-17 0.7568693152871191 1102 18-19 0.6737091776095525 1102 20-21 0.6279863002701305 1102 22-23 0.6019065006213964 1102 24-25 0.589095880318844 1102 26-27 0.5579406545719436 1102 28-29 0.6126333672558388 1102 30-31 0.7201997862139251 1102 32-33 0.7076209417272494 1102 34-35 0.6737573827670857 1102 36-37 0.6980731614415774 1102 38-39 0.6912637845654217 1102 40-41 0.7934010636549047 1102 42-43 0.7617160792360025 1102 44-45 0.7246237065258754 1102 46-47 0.85879963782223 1102 48-49 0.8118245479985902 1102 50-51 0.8444020671062447 1102 52-53 0.8426539493812513 1102 54-55 0.7278895926867008 1102 56-57 0.8101633177496481 1102 58-59 0.7222246142802788 1102 60-61 0.784069231559748 1102 62-63 0.6009435835119916 1102 64-65 0.5871165833101983 1102 66-67 0.6729285149716517 1102 68-69 0.7647098957034615 1102 70-71 0.7020108511868877 1102 72-73 0.6326761710449489 1102 74-75 0.5122123545297477 1102 76-77 0.6696175464156013 1102 78-79 0.4482292929105185 1102 80-81 0.45769429614614765 1102 82-83 0.4232086482519506 1102 84-85 0.4323538610602391 1102 86-87 0.4868689946222524 1102 88-89 0.5421393584603145 1102 90-91 0.4109469841017379 1102 92-93 0.36832845727964525 1102 94-95 0.40964613965174124 1102 96-97 0.36751732078473864 1102 98-99 0.16887564203436156 1102 100-101 0.4470493667570352 1103 1 -0.015765767454393398 1103 2 0.16786991623154535 1103 3 0.2545441106961732 1103 4 0.24172673911760967 1103 5 0.23779233953788292 1103 6 0.1029618693476948 1103 7 0.15979585237879235 1103 8 0.1586857398434418 1103 9 0.24487054207808256 1103 10-11 0.18513640915736573 1103 12-13 0.25317001461696975 1103 14-15 0.2920775585304938 1103 16-17 0.3784047580146179 1103 18-19 0.37545065418546386 1103 20-21 0.3412558612218106 1103 22-23 0.35981672873030135 1103 24-25 0.406118200652422 1103 26-27 0.39286829744660423 1103 28-29 0.4310047187397714 1103 30-31 0.5611367496674404 1103 32-33 0.4410033307387735 1103 34-35 0.5057416849500598 1103 36-37 0.6162728180518329 1103 38-39 0.6244254230250661 1103 40-41 0.6593564229877487 1103 42-43 0.5665971192163823 1103 44-45 0.6138805130012202 1103 46-47 0.5891160469465859 1103 48-49 0.60763028751294 1103 50-51 0.6559056236429157 1103 52-53 0.55371600481417 1103 54-55 0.5411100979601926 1103 56-57 0.56451700918943 1103 58-59 0.6421412196273479 1103 60-61 0.5373191702401456 1103 62-63 0.5193885185132245 1103 64-65 0.45233610747011 1103 66-67 0.4405272395240232 1103 68-69 0.48067751895585076 1103 70-71 0.47598681390457287 1103 72-73 0.420510362852653 1103 74-75 0.32886675727686665 1103 76-77 0.2741061409704173 1103 78-79 0.35355183399464707 1103 80-81 0.3955162240342993 1103 82-83 0.3772189499443712 1103 84-85 0.24569700552921248 1103 86-87 0.26918442263304243 1103 88-89 0.30569098956161156 1103 90-91 0.23900683202913697 1103 92-93 0.06933409868263851 1103 94-95 0.2099895293991061 1103 96-97 0.13916313878817022 1103 98-99 0.15575324811726432 1103 100-101 0.3684376424499476 1104 1 0.16249030318578406 1104 2 0.13539957555277127 1104 3 0.1224326510315592 1104 4 0.16170466406241957 1104 5 0.15810913594390996 1104 6 0.1785560239763555 1104 7 0.18640371677857104 1104 8 0.23451226347408038 1104 9 0.3697930857440923 1104 10-11 0.27833574303289055 1104 12-13 0.32623150849147464 1104 14-15 0.3917049612905146 1104 16-17 0.46561413539478025 1104 18-19 0.368561074644262 1104 20-21 0.43226786049888943 1104 22-23 0.3401339446914662 1104 24-25 0.3084481839993103 1104 26-27 0.3042267847243991 1104 28-29 0.24494976347080666 1104 30-31 0.3846226397052632 1104 32-33 0.3633662653010816 1104 34-35 0.49387553594988987 1104 36-37 0.5053384042026394 1104 38-39 0.5604045356336727 1104 40-41 0.5403189470314871 1104 42-43 0.4583494719525234 1104 44-45 0.4197813947124871 1104 46-47 0.46291708744333704 1104 48-49 0.5548446027343985 1104 50-51 0.6505942169067964 1104 52-53 0.6166323326093774 1104 54-55 0.618618069507896 1104 56-57 0.6204388686578213 1104 58-59 0.6386669803130971 1104 60-61 0.5335665722256167 1104 62-63 0.516674319476266 1104 64-65 0.6358955164782927 1104 66-67 0.5867551160586189 1104 68-69 0.6018781244061984 1104 70-71 0.6172865311889488 1104 72-73 0.595419674394936 1104 74-75 0.5138284486650129 1104 76-77 0.5863463395168225 1104 78-79 0.4584535289974241 1104 80-81 0.33424180915235624 1104 82-83 0.3752970646572038 1104 84-85 0.46837186320319546 1104 86-87 0.40691469385930645 1104 88-89 0.571337975864747 1104 90-91 0.5587643291567979 1104 92-93 0.6446321442011467 1104 94-95 0.78835274796797 1104 96-97 0.6543284435143022 1104 98-99 0.8948665990073685 1104 100-101 1.1155491666615092 1105 1 0.09967821855022763 1105 2 0.2217537841694721 1105 3 0.20559203942602622 1105 4 0.31116401672255023 1105 5 0.2594457704496733 1105 6 0.26548122576193833 1105 7 0.21311820884989174 1105 8 0.21000257077050577 1105 9 0.2470893480076839 1105 10-11 0.3158395480784293 1105 12-13 0.2800558628626817 1105 14-15 0.31989512564958034 1105 16-17 0.28566076965474707 1105 18-19 0.3373052252730133 1105 20-21 0.38009016912636184 1105 22-23 0.3481544371069063 1105 24-25 0.33608214996281305 1105 26-27 0.3314537762521539 1105 28-29 0.4165027230872198 1105 30-31 0.40359953622504463 1105 32-33 0.41002604979842516 1105 34-35 0.32947145334873795 1105 36-37 0.4309312113903445 1105 38-39 0.5199254685835726 1105 40-41 0.49932300826765896 1105 42-43 0.43865434354523813 1105 44-45 0.5546842029779029 1105 46-47 0.6793398057867748 1105 48-49 0.7008405367714516 1105 50-51 0.8764348956323857 1105 52-53 0.850356309406628 1105 54-55 0.7406768402087636 1105 56-57 0.7500673165962866 1105 58-59 0.744279209776721 1105 60-61 0.7216032963508283 1105 62-63 0.754497937417554 1105 64-65 0.6510321530638237 1105 66-67 0.588945735132441 1105 68-69 0.6515035992097111 1105 70-71 0.5027768762994889 1105 72-73 0.630499830869752 1105 74-75 0.5512676152040576 1105 76-77 0.590496084366066 1105 78-79 0.6132132391900278 1105 80-81 0.7064728704244594 1105 82-83 0.640171956650633 1105 84-85 0.6626403538338046 1105 86-87 0.5949739882856946 1105 88-89 0.6054055411839094 1105 90-91 0.7352517947223163 1105 92-93 0.7315605365528626 1105 94-95 0.7750811745414623 1105 96-97 0.9816408240453214 1105 98-99 0.9287181511823412 1105 100-101 1.2172975023676784 1106 1 0.08085471837671676 1106 2 0.05604735940670125 1106 3 0.1003639822588589 1106 4 0.11640089767261941 1106 5 0.17711292185438765 1106 6 0.1555415649326477 1106 7 0.14074768475366994 1106 8 0.07534799565046768 1106 9 0.068023784336674 1106 10-11 0.10996307967099028 1106 12-13 0.13447957635742114 1106 14-15 0.33191929688659627 1106 16-17 0.3155888410038159 1106 18-19 0.2371568853982282 1106 20-21 0.2968997377551048 1106 22-23 0.2173257242893456 1106 24-25 0.15758277902785522 1106 26-27 0.10238871419154094 1106 28-29 0.02389500111436149 1106 30-31 0.008965707255036648 1106 32-33 0.08684702094351593 1106 34-35 0.21748431007922164 1106 36-37 0.28691704226464765 1106 38-39 0.311810848783729 1106 40-41 0.41461269739233586 1106 42-43 0.42498785426624863 1106 44-45 0.3660775535392702 1106 46-47 0.4737785539131565 1106 48-49 0.5300219040428331 1106 50-51 0.40582510383261905 1106 52-53 0.32357464627632027 1106 54-55 0.23539704876925072 1106 56-57 0.36818402491941526 1106 58-59 0.4869477178095565 1106 60-61 0.5754626411473325 1106 62-63 0.5647783399097364 1106 64-65 0.58774699028897 1106 66-67 0.584640834900469 1106 68-69 0.5288682362239001 1106 70-71 0.5395282740824676 1106 72-73 0.5446508073911964 1106 74-75 0.4497695443520442 1106 76-77 0.5048587062744936 1106 78-79 0.45539002083036095 1106 80-81 0.4115471042031764 1106 82-83 0.5418650969460437 1106 84-85 0.6840452095136555 1106 86-87 0.7146072130431769 1106 88-89 0.845776103584928 1106 90-91 0.8588365693875275 1106 92-93 0.9064904364998299 1106 94-95 1.0465239360070235 1106 96-97 1.0849283329888095 1106 98-99 1.116311946626535 1106 100-101 1.540343718469643 1107 1 0.09294977208864807 1107 2 0.1260713545810539 1107 3 0.1866438209899961 1107 4 0.24295903163753962 1107 5 0.1549024163146484 1107 6 0.06526848122009454 1107 7 0.05247915169709927 1107 8 0.07448155252480149 1107 9 0.14634840223237688 1107 10-11 0.146643928328416 1107 12-13 0.1535162100251739 1107 14-15 0.31505930495891477 1107 16-17 0.29673011980024455 1107 18-19 0.18079833051979932 1107 20-21 0.2126146919380716 1107 22-23 0.18263872603686337 1107 24-25 0.09525121095874312 1107 26-27 0.18824375850056185 1107 28-29 0.18685871562057343 1107 30-31 0.11546530585701476 1107 32-33 0.2066657154463556 1107 34-35 0.3106169111383892 1107 36-37 0.1999573217795998 1107 38-39 0.1231727966951297 1107 40-41 0.24226297770565708 1107 42-43 0.17987908870930625 1107 44-45 0.37541970432299365 1107 46-47 0.4700599117472919 1107 48-49 0.4642769558919255 1107 50-51 0.2695317927646812 1107 52-53 0.22301634820392024 1107 54-55 0.14020262576226372 1107 56-57 0.3915701165889658 1107 58-59 0.45635680892025476 1107 60-61 0.40756455854361207 1107 62-63 0.39764962198830744 1107 64-65 0.3755841313593109 1107 66-67 0.1765503917215696 1107 68-69 0.4541085760594399 1107 70-71 0.38063154203893035 1107 72-73 0.3316602036530938 1107 74-75 0.35512892863853196 1107 76-77 0.14497184063860047 1107 78-79 0.2707312424109354 1107 80-81 0.31423003968676255 1107 82-83 0.11148564275864103 1107 84-85 0.3193412350079683 1107 86-87 0.2570289404296098 1107 88-89 0.4527916427888705 1107 90-91 0.5514231249933488 1107 92-93 0.5881345716534234 1107 94-95 0.7070609111957467 1107 96-97 0.6451979127849583 1107 98-99 0.9482877895623716 1107 100-101 1.3051361779528392 1108 1 0.16570240330469943 1108 2 0.1627415426692913 1108 3 0.1321277209520737 1108 4 0.09858653418626062 1108 5 0.2279205085414162 1108 6 0.15297638053205986 1108 7 0.17620319044751653 1108 8 0.260958185553271 1108 9 0.24104404551975733 1108 10-11 0.35781585373337776 1108 12-13 0.3124745649056706 1108 14-15 0.4259877474563183 1108 16-17 0.5029328083933038 1108 18-19 0.5421183772586744 1108 20-21 0.4940710213855297 1108 22-23 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2316 50-51 -2.2397714326582303 2316 52-53 -2.5734145514049516 2316 54-55 -2.4169703220126983 2316 56-57 -2.1390278391808764 2316 58-59 -1.8614489748653043 2316 60-61 -1.7280392703904894 2316 62-63 -2.058013522829775 2316 64-65 -2.1076410148035585 2316 66-67 -1.8427104608199798 2316 68-69 -2.0492907355606107 2316 70-71 -1.951883750737796 2316 72-73 -2.0062758762301414 2316 74-75 -1.953438357529091 2316 76-77 -1.9103448079883307 2316 78-79 -1.7064849289829596 2316 80-81 -1.729086237895583 2316 82-83 -1.7473959038207774 2316 84-85 -1.719086837692167 2316 86-87 -1.8639006902477817 2316 88-89 -1.962219929292175 2316 90-91 -2.101573726252621 2316 92-93 -1.8985408278857463 2316 94-95 -1.9279827634166047 2316 96-97 -2.095318925669197 2316 98-99 -2.3860472103966295 2316 100-101 -2.307355570917629 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22708.0 3 6187.0 4 5805.0 5 6410.0 6 7560.0 7 8617.0 8 10684.0 9 12443.0 10 15031.0 11 17692.0 12 20913.0 13 24603.0 14 26377.0 15 28509.0 16 32640.0 17 37837.0 18 34900.0 19 29063.0 20 29643.0 21 31419.0 22 34358.0 23 38311.0 24 43445.0 25 49417.0 26 56538.0 27 65188.0 28 75989.0 29 89178.0 30 106140.0 31 126929.0 32 155592.0 33 194675.0 34 255268.0 35 352806.0 36 513327.0 37 741327.0 38 659635.0 39 107038.0 40 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.895951554913225 18.690462595053102 14.165102958607845 23.248482891425827 2 15.410091023164885 20.21956289999646 40.16094756137377 24.20939851546489 3 19.724831896226306 24.204877696097295 29.440901714470364 26.629388693206035 4 12.7195089391735 16.35990935199658 37.66870505029745 33.25187665853247 5 13.624579115467148 37.35151872705524 34.47811302019803 14.545789137279582 6 31.83666704128204 36.06257437488211 17.643351245194452 14.457407338641396 7 28.27631272705443 32.5532395436252 20.980309278705608 18.190138450614754 8 26.045492425351497 34.56483446887654 20.05732379232637 19.3323493134456 9 26.086625215235674 17.045067677101628 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34-35 22.47086694957579 26.821361424719015 28.88064917605951 21.827122449645685 36-37 22.394507112537656 26.50413075487158 29.037783623605367 22.063578508985394 38-39 22.01798334711014 26.918907398943766 29.038239558682406 22.024869695263696 40-41 22.335688294454574 26.793974376482883 28.926529572308013 21.94380775675453 42-43 22.03567948577391 26.87475593847124 28.609603798059336 22.479960777695517 44-45 22.227504581649455 26.859713564984027 28.691042832201003 22.221739021165522 46-47 22.496055356466783 27.078630325638375 28.229048173870595 22.196266144024243 48-49 22.379554981151124 27.105859319547626 28.22619441947034 22.288391279830915 50-51 22.229582401810603 27.30777363110517 28.307805174731975 22.154838792352248 52-53 22.507100672134943 27.28478010927106 27.98561222881692 22.222506989777077 54-55 22.098287166327143 27.108798167825615 28.246231206548632 22.54668345929861 56-57 22.29225923357309 27.289986486702887 27.998011808428995 22.419742471295024 58-59 22.463827282779892 27.064577947712078 28.049737924909124 22.42185684459891 60-61 22.19115730878212 27.108392500297928 28.49678484301426 22.20366534790569 62-63 22.12679506974176 27.112909300047072 28.48723276684386 22.273062863367304 64-65 21.91339839780936 27.283996640587887 28.264868571935597 22.53773638966716 66-67 21.97052530909234 27.24942322481415 28.033899098850394 22.746152367243113 68-69 22.105092946131325 27.11543837040711 28.223707660050884 22.555761023410682 70-71 22.174652479799292 26.971563285361388 28.029255699710898 22.824528535128422 72-73 22.106451813263085 26.90131607486513 28.250701959175945 22.741530152695837 74-75 21.96257035142099 27.46173440937586 27.868620743069727 22.707074496133423 76-77 22.138062591469897 27.08444173267367 27.93946272932007 22.83803294653636 78-79 22.170769803083942 27.17962646800145 27.956266059634054 22.693337669280552 80-81 22.304628092555692 27.212790006498487 27.762297076297155 22.720284824648665 82-83 22.342196160037258 27.163581279334586 27.952371007893195 22.54185155273496 84-85 22.21773610797788 27.24958334375462 27.83427584855015 22.69840469971735 86-87 22.187300221921912 27.22228991431249 27.868097095672578 22.722312768093015 88-89 22.19246530336553 27.2068360634307 27.60199962223389 22.99869901096988 90-91 22.229161855274473 27.194715778893432 27.693075366304587 22.883046999527508 92-93 22.472397105561097 27.11745222138709 27.752503956826686 22.657646716225127 94-95 22.2529489163438 26.96653170365455 27.840139081084786 22.940380298916864 96-97 22.210360307508157 26.743611081257757 28.032499455244785 23.0135291559893 98-99 22.237101890810678 26.92063574226212 27.609077172977997 23.233185193949208 100-101 22.5368976967794 26.35163415394749 27.988739005418452 23.12272914385466 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3694.0 1 2477.0 2 1244.5 3 1253.0 4 1200.0 5 1219.0 6 1658.5 7 2769.5 8 4074.0 9 4630.0 10 4196.0 11 3662.5 12 3631.5 13 3805.0 14 4114.0 15 4490.5 16 4912.5 17 5257.5 18 5670.0 19 6260.5 20 7054.5 21 8227.5 22 9889.5 23 12075.5 24 14694.0 25 18238.5 26 23619.0 27 30184.0 28 36189.0 29 42754.0 30 49932.5 31 58466.0 32 68773.0 33 75795.5 34 81644.5 35 89151.0 36 98357.0 37 106851.0 38 112164.0 39 115992.5 40 120597.0 41 126486.0 42 129923.0 43 132917.0 44 137526.0 45 143404.0 46 147082.0 47 153105.0 48 167773.0 49 167293.0 50 152329.0 51 143775.5 52 138780.0 53 135468.5 54 132302.0 55 124452.5 56 113256.0 57 101774.5 58 92195.5 59 81658.0 60 67483.5 61 55019.5 62 45116.0 63 34837.5 64 25268.5 65 18817.0 66 14063.5 67 10811.0 68 8247.0 69 6054.0 70 4545.0 71 3294.0 72 2463.0 73 2039.5 74 1599.5 75 1155.5 76 839.0 77 547.0 78 379.0 79 291.0 80 212.0 81 150.0 82 119.0 83 92.0 84 64.0 85 47.0 86 36.5 87 26.0 88 23.5 89 20.5 90 13.5 91 9.5 92 15.0 93 14.5 94 6.0 95 5.0 96 4.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27636974836117156 2 0.1648400737116899 3 0.19304156934818956 4 0.16322014445841837 5 0.2355769844378796 6 0.1928943030524376 7 0.23803142270041228 8 0.24473203915712627 9 0.1481744379090935 10-11 0.20391473085120904 12-13 0.2098544714465379 14-15 0.17145478482921528 16-17 0.28370851876614406 18-19 0.22973542137305203 20-21 0.19635506100260858 22-23 0.24314892647779274 24-25 0.23211622648770866 26-27 0.3410319243875931 28-29 0.3127199790292795 30-31 0.2876110756035709 32-33 0.1901698765810264 34-35 0.35944021135658766 36-37 0.3995089159924325 38-39 0.38036429754467815 40-41 0.342602764875614 42-43 0.37643719632462597 44-45 0.38461047573885954 46-47 0.3902679559339972 48-49 0.4102838999649506 50-51 0.40203698740284105 52-53 0.4015829163242725 54-55 0.3957290810681323 56-57 0.37520997719335963 58-59 0.3497942689848345 60-61 0.3157389380921946 62-63 0.3829046411463994 64-65 0.41711951052610396 66-67 0.4224579137471124 68-69 0.4267286363239191 70-71 0.42710907425461164 72-73 0.41813810240505495 74-75 0.413658752575933 76-77 0.39818351933066487 78-79 0.3721419293651939 80-81 0.38324826250315397 82-83 0.3929432936401578 84-85 0.39394961332779616 86-87 0.3888443817417283 88-89 0.3972631049822152 90-91 0.41826082431818157 92-93 0.4178312976222384 94-95 0.4255505059579034 96-97 0.4266795475586684 98-99 0.42368513287837867 100-101 0.3969685723907112 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4074252.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.66485740553865 #Duplication Level Percentage of deduplicated Percentage of total 1 73.84284201627729 40.36608429239516 2 14.172243908568305 15.494473847567999 3 5.254942717996693 8.617820830606886 4 2.3903869076532356 5.2268063780372245 5 1.2786971063133017 3.4949897490745765 6 0.7541744656265746 2.4736103773425 7 0.48039786228414544 1.838261644778188 8 0.3397772604539132 1.4859100393886184 9 0.23644997936186013 1.1632953964822774 >10 1.1457482684630231 11.056151425738323 >50 0.06889005250246791 2.5749266837350357 >100 0.031355926596571077 3.015769174426073 >500 0.0026540456357697093 1.0536716042323577 >1k 0.0012595469834377783 1.250836901901682 >5k 1.3495146251118995E-4 0.5784877841672793 >10k+ 4.498382083706332E-5 0.3089038701257965 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12562 0.3083265345393461 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9661 0.23712328054327517 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7704 0.18908992374551206 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6160 0.1511933969720086 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4461 0.10949249089157961 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.4544382625326074E-5 0.0 5 2.4544382625326074E-5 0.0 0.0 2.4544382625326074E-5 0.0 6 2.4544382625326074E-5 0.0 0.0 2.4544382625326074E-5 0.0 7 4.908876525065215E-5 0.0 0.0 4.908876525065215E-5 0.0 8 4.908876525065215E-5 0.0 0.0 7.363314787597822E-5 0.0 9 4.908876525065215E-5 0.0 0.0 7.363314787597822E-5 0.0 10-11 4.908876525065215E-5 0.0 0.0 7.363314787597822E-5 0.0 12-13 4.908876525065215E-5 0.0 0.0 9.81775305013043E-5 0.0 14-15 4.908876525065215E-5 0.0 0.0 1.2272191312663036E-4 7.363314787597822E-5 16-17 4.908876525065215E-5 0.0 0.0 1.8408286968994557E-4 9.81775305013043E-5 18-19 7.363314787597822E-5 0.0 0.0 1.963550610026086E-4 9.81775305013043E-5 20-21 9.81775305013043E-5 0.0 0.0 2.5771601756592377E-4 9.81775305013043E-5 22-23 9.81775305013043E-5 0.0 0.0 3.681657393798911E-4 1.8408286968994554E-4 24-25 1.2272191312663036E-4 0.0 0.0 5.277042264445105E-4 2.6998820887858677E-4 26-27 1.2272191312663036E-4 0.0 0.0 9.326865397623908E-4 2.6998820887858677E-4 28-29 1.5953848706461946E-4 0.0 0.0 0.002785787427974509 2.822604001912498E-4 30-31 1.718106783772825E-4 0.0 0.0 0.008713255831990755 3.190769741292389E-4 32-33 1.718106783772825E-4 0.0 0.0 0.02134134069272102 3.927101220052172E-4 34-35 2.0862725231527162E-4 0.0 0.0 0.04432715502133888 4.417988872558693E-4 36-37 2.454438262532607E-4 0.0 0.0 0.08197823796858908 4.5407107856853233E-4 38-39 3.190769741292389E-4 0.0 0.0 0.14001343068617259 4.908876525065214E-4 40-41 3.190769741292389E-4 0.0 0.0 0.20149710916261437 5.031598438191844E-4 42-43 3.6816573937989113E-4 0.0 0.0 0.26784057539887074 5.277042264445105E-4 44-45 3.927101220052172E-4 0.0 0.0 0.34970836364564584 5.645208003824996E-4 46-47 4.5407107856853233E-4 0.0 0.0 0.45081894787067667 5.890651830078257E-4 48-49 4.663432698811954E-4 0.0 0.0 0.557955178030225 6.381539482584778E-4 50-51 4.663432698811954E-4 0.0 0.0 0.6735592201955107 6.8724271350913E-4 52-53 4.663432698811954E-4 0.0 0.0 0.7914213455623265 6.99514904821793E-4 54-55 4.663432698811954E-4 0.0 0.0 0.924918242661475 7.11787096134456E-4 56-57 4.663432698811954E-4 0.0 0.0 1.0857207654312988 7.11787096134456E-4 58-59 4.663432698811954E-4 0.0 0.0 1.262747125116463 7.24059287447119E-4 60-61 4.663432698811954E-4 0.0 0.0 1.4532115342889935 7.363314787597822E-4 62-63 4.663432698811954E-4 0.0 0.0 1.6441913754966557 7.363314787597822E-4 64-65 4.663432698811954E-4 0.0 0.0 1.8471979641907275 7.486036700724453E-4 66-67 4.908876525065214E-4 0.0 0.0 2.08675113861391 7.608758613851083E-4 68-69 4.908876525065214E-4 0.0 0.0 2.3576597618409467 7.608758613851083E-4 70-71 5.031598438191844E-4 0.0 0.0 2.6451849321053285 8.345090092610865E-4 72-73 5.154320351318475E-4 1.2272191312663037E-5 0.0 2.941153369992823 9.817753050130429E-4 74-75 5.154320351318475E-4 2.4544382625326074E-5 0.0 3.243896057484908 9.817753050130429E-4 76-77 5.154320351318475E-4 2.4544382625326074E-5 0.0 3.575441577987812 9.817753050130429E-4 78-79 5.277042264445105E-4 2.4544382625326074E-5 0.0 3.9546768339317255 0.0010063196876383689 80-81 5.890651830078257E-4 2.4544382625326074E-5 0.0 4.364960733896677 0.0011167694094523363 82-83 5.890651830078257E-4 2.4544382625326074E-5 0.0 4.780116693812754 0.0012272191312663037 84-85 5.890651830078257E-4 2.4544382625326074E-5 0.0 5.200058808340771 0.0012272191312663037 86-87 5.890651830078257E-4 2.4544382625326074E-5 0.0 5.634838002165797 0.0012272191312663037 88-89 5.890651830078257E-4 2.4544382625326074E-5 0.0 6.113919806629536 0.0012272191312663037 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 23005 0.0 23.654945 1 GTACATG 14845 0.0 23.243156 1 TACATGG 14695 0.0 23.115385 2 ACATGGG 14940 0.0 21.688416 3 GGTATCA 17365 0.0 21.402803 1 GAGTACT 12620 0.0 21.082262 12-13 ATCAACG 26550 0.0 20.245249 3 TCAACGC 26690 0.0 20.174604 4 TATCAAC 26715 0.0 20.173485 2 CAACGCA 26745 0.0 20.115377 5 AACGCAG 27330 0.0 19.64888 6 GTACTTT 13960 0.0 19.025074 14-15 GTGTAGC 2705 0.0 18.975586 1 AGTACTT 13470 0.0 18.95901 12-13 CATGGGG 7490 0.0 18.938566 4 AGAGTAC 20630 0.0 18.739504 10-11 GAGTACA 13010 0.0 18.082838 1 TAAGGTG 1775 0.0 17.907469 5 ACGCAGA 30005 0.0 17.771103 7 TACCTGG 2905 0.0 17.637432 2 >>END_MODULE