FastQCFastQC Report
Thu 26 May 2016
SRR938710_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938710_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4074252
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119970.2944589583560369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104040.25535975683389245No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT87850.21562240136348954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67220.16498734000744186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG147450.021.9342381
GTATCAA252450.021.8356691
TACATGG143250.021.432892
GAGTACT136200.020.74517412-13
GGTATCA193600.020.3660871
ACATGGG143150.020.0555743
GTACTTT145200.019.41030114-15
TCAACGC287700.018.9023514
ATCAACG288100.018.8596363
CAACGCA290200.018.723165
TATCAAC291550.018.7178482
AGAGTAC216750.018.54115310-11
AGTACTT146650.018.44188112-13
AACGCAG296150.018.3749776
GAGTACA128300.017.3120571
AGTACAT120200.016.9759882
GTATAGG11500.016.9568211
TAGCCCT33450.016.8819244
GTGTAGC24550.016.8549021
ACTTTTT170650.016.70934116-17
Produced by FastQC (version 0.11.5)