##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938710_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4074252 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.074531226836235 33.0 31.0 34.0 30.0 34.0 2 32.278181369242745 34.0 31.0 34.0 30.0 34.0 3 32.362195072862455 34.0 31.0 34.0 30.0 34.0 4 35.97127816345184 37.0 35.0 37.0 35.0 37.0 5 35.878041662616845 37.0 35.0 37.0 35.0 37.0 6 35.90112810891423 37.0 35.0 37.0 35.0 37.0 7 35.82394314342854 37.0 35.0 37.0 33.0 37.0 8 35.8077885216722 37.0 35.0 37.0 33.0 37.0 9 37.4601065422561 39.0 37.0 39.0 34.0 39.0 10-11 37.38771693552583 39.0 37.0 39.0 34.0 39.0 12-13 37.30810084894111 39.0 37.0 39.0 34.0 39.0 14-15 38.53842742176969 40.0 38.0 41.0 34.0 41.0 16-17 38.46364817394702 40.0 38.0 41.0 33.5 41.0 18-19 38.60985869308035 40.0 38.0 41.0 34.0 41.0 20-21 38.60952660758343 40.0 38.0 41.0 34.0 41.0 22-23 38.537547996540226 40.0 38.0 41.0 34.0 41.0 24-25 38.46369861265332 40.0 38.0 41.0 34.0 41.0 26-27 38.28747559061148 40.0 38.0 41.0 33.5 41.0 28-29 38.12583254545865 40.0 38.0 41.0 33.0 41.0 30-31 37.915731034800984 40.0 37.0 41.0 33.0 41.0 32-33 37.769840083529445 40.0 37.0 41.0 33.0 41.0 34-35 37.74339056592474 40.0 37.0 41.0 33.0 41.0 36-37 37.63234515194445 40.0 37.0 41.0 32.5 41.0 38-39 37.51053003103392 40.0 37.0 41.0 32.0 41.0 40-41 37.34168075514229 40.0 36.5 41.0 31.5 41.0 42-43 37.155091658542474 40.0 36.0 41.0 31.0 41.0 44-45 36.923381396143384 40.0 36.0 41.0 31.0 41.0 46-47 36.640689873871324 39.5 36.0 41.0 30.0 41.0 48-49 36.35473848942088 39.0 35.0 41.0 30.0 41.0 50-51 36.1254982509673 39.0 35.0 41.0 29.0 41.0 52-53 35.90769557209519 39.0 35.0 40.5 28.5 41.0 54-55 35.64109031547386 39.0 35.0 40.0 28.0 41.0 56-57 35.36913143811428 38.0 34.5 40.0 28.0 41.0 58-59 35.064153370974594 38.0 34.0 40.0 27.0 41.0 60-61 34.7027946479501 38.0 34.0 40.0 26.0 41.0 62-63 34.34221680445883 37.0 34.0 40.0 26.0 41.0 64-65 33.95227430703845 36.5 33.0 39.5 25.5 41.0 66-67 33.54638053807177 36.0 33.0 39.0 25.0 40.5 68-69 33.19571997510218 36.0 32.5 39.0 23.5 40.0 70-71 32.759285262669074 35.0 32.0 38.5 23.0 40.0 72-73 32.26795924748886 35.0 31.5 37.5 22.0 40.0 74-75 31.776094114944286 35.0 31.0 37.0 20.5 39.0 76-77 29.840489616253485 33.0 28.5 35.0 18.0 37.5 78-79 30.799316537121417 34.0 30.5 36.0 18.0 38.0 80-81 30.84532387785537 34.0 31.0 36.0 18.0 37.5 82-83 30.517559296773985 34.0 30.5 35.0 17.5 37.0 84-85 30.103694984993567 34.0 30.0 35.0 12.0 36.5 86-87 29.517335329282528 34.0 29.5 35.0 6.5 36.0 88-89 28.90282363486598 34.0 29.0 35.0 2.0 36.0 90-91 28.376338773350298 33.5 28.5 35.0 2.0 35.0 92-93 28.06829903992193 33.0 28.0 35.0 2.0 35.0 94-95 27.712614855438495 33.0 27.0 35.0 2.0 35.0 96-97 27.089660629730318 33.0 26.5 35.0 2.0 35.0 98-99 26.224522562669172 33.0 25.0 35.0 2.0 35.0 100-101 22.44471684618428 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1275711336081713 1101 2 0.14312259604837152 1101 3 0.07964449020184361 1101 4 0.08432733479011745 1101 5 0.010555629819513968 1101 6 0.050736174542187484 1101 7 0.12967610537199903 1101 8 0.20107947392592251 1101 9 0.11337006484122725 1101 10-11 0.1906177366213555 1101 12-13 0.1253502103888522 1101 14-15 0.2641284647685538 1101 16-17 0.3231627587243011 1101 18-19 0.3225014807298905 1101 20-21 0.2587312295371831 1101 22-23 0.1658230219170136 1101 24-25 0.2410275998475555 1101 26-27 0.19881243331858656 1101 28-29 0.19459115586818143 1101 30-31 0.2704509978531533 1101 32-33 0.29847321162714735 1101 34-35 0.33112664185742346 1101 36-37 0.2975430587098984 1101 38-39 0.3899524679712343 1101 40-41 0.39446655248833196 1101 42-43 0.43249696022731143 1101 44-45 0.4136233465832362 1101 46-47 0.39057305563239453 1101 48-49 0.26717058387762904 1101 50-51 0.2987694104759768 1101 52-53 0.21910226578452097 1101 54-55 0.3802429175271911 1101 56-57 0.3322340170651614 1101 58-59 0.4092201038134746 1101 60-61 0.5010999162346934 1101 62-63 0.4068077985058096 1101 64-65 0.29383783036615796 1101 66-67 0.24792578544661836 1101 68-69 0.20938352251663872 1101 70-71 0.23347255039064407 1101 72-73 0.17754620670937982 1101 74-75 0.1092253238391585 1101 76-77 0.19753074149702954 1101 78-79 0.07294607008702414 1101 80-81 0.19684369576114236 1101 82-83 0.23406675607687433 1101 84-85 0.20734216651213444 1101 86-87 0.3094271998749676 1101 88-89 0.4051103229902644 1101 90-91 0.26945508626696935 1101 92-93 0.31817792360068253 1101 94-95 0.32532867780104624 1101 96-97 0.3582745459072285 1101 98-99 0.09478771021746368 1101 100-101 0.6650027201974034 1102 1 0.25434129193141075 1102 2 0.18593730861314128 1102 3 0.19134737347062725 1102 4 0.16423726953875928 1102 5 -0.057520139901200196 1102 6 0.018249325541773942 1102 7 0.13957378837372403 1102 8 0.17848166536357724 1102 9 0.2564270126407493 1102 10-11 0.2324873361602542 1102 12-13 0.30802722622117784 1102 14-15 0.35982434634352245 1102 16-17 0.3906977595855139 1102 18-19 0.37860733022390036 1102 20-21 0.3641387964059817 1102 22-23 0.3376042603720393 1102 24-25 0.40427272425482386 1102 26-27 0.28656917680060445 1102 28-29 0.28085453130053395 1102 30-31 0.3018078620876068 1102 32-33 0.30948742864435275 1102 34-35 0.36146207070812864 1102 36-37 0.2869074407574743 1102 38-39 0.35319722719975744 1102 40-41 0.35110465000445146 1102 42-43 0.36723924445688283 1102 44-45 0.240779407546178 1102 46-47 0.2029146669592663 1102 48-49 0.16432124628143896 1102 50-51 0.20447456759109173 1102 52-53 0.15048213627996176 1102 54-55 0.19234676705894316 1102 56-57 0.11325853820930831 1102 58-59 0.160596492938204 1102 60-61 0.18068286384797716 1102 62-63 0.1004073410585633 1102 64-65 0.19638595636012468 1102 66-67 0.3429401826186762 1102 68-69 0.23259635295014647 1102 70-71 0.27984659932824485 1102 72-73 0.2823877160905184 1102 74-75 0.23040149154384792 1102 76-77 0.23510583898453774 1102 78-79 0.12393579578300873 1102 80-81 0.2617784109308481 1102 82-83 0.26031884748598344 1102 84-85 0.42350905563352015 1102 86-87 0.5390579250408365 1102 88-89 0.45908669349087816 1102 90-91 0.5102421742768719 1102 92-93 0.45523102850253494 1102 94-95 0.5732753994392823 1102 96-97 0.7830713391509363 1102 98-99 1.0347049728862743 1102 100-101 1.726179198304024 1103 1 0.1515696461134084 1103 2 0.13124022742599095 1103 3 0.12315998077992418 1103 4 0.01878718369132315 1103 5 -0.12399058385508965 1103 6 0.013589036113273778 1103 7 -0.04193222094685467 1103 8 -0.0047412926938221744 1103 9 0.042298977320662345 1103 10-11 0.09341448847813894 1103 12-13 0.12173828827661026 1103 14-15 0.1572243953992043 1103 16-17 0.10911816674764196 1103 18-19 -0.052714720303448814 1103 20-21 0.10009660852273328 1103 22-23 0.08386376490968672 1103 24-25 0.0679960502298087 1103 26-27 0.058950829976957664 1103 28-29 0.020000962499949537 1103 30-31 0.08596287803069913 1103 32-33 0.1028403842489567 1103 34-35 0.11348561743365337 1103 36-37 0.15372127445872508 1103 38-39 0.10215479403812111 1103 40-41 0.22993712486341877 1103 42-43 0.23932656356407023 1103 44-45 0.23562856133573007 1103 46-47 0.2491496436578231 1103 48-49 0.2108015013709661 1103 50-51 0.26812431004878334 1103 52-53 0.280479683496047 1103 54-55 0.22668574179527212 1103 56-57 0.1379090165021779 1103 58-59 0.25159477598947433 1103 60-61 0.3334913014246226 1103 62-63 0.1374443780956014 1103 64-65 0.16216962081929154 1103 66-67 0.1565286054786199 1103 68-69 0.13583402771100594 1103 70-71 0.20074431823920946 1103 72-73 0.2339452338732002 1103 74-75 0.4314071329468341 1103 76-77 0.39208400920774267 1103 78-79 0.15238808913596458 1103 80-81 0.3293526076441182 1103 82-83 0.30692174177099574 1103 84-85 0.3854909050325901 1103 86-87 0.4122489969074046 1103 88-89 0.28395792233562034 1103 90-91 0.2848313329719936 1103 92-93 0.40421312317999636 1103 94-95 0.505210645944068 1103 96-97 0.5050482829821838 1103 98-99 0.7276164273871295 1103 100-101 1.3143567798590894 1104 1 0.1244050643130663 1104 2 0.1148114162516265 1104 3 0.07745428903031382 1104 4 0.07505598298905625 1104 5 -0.05181903098468865 1104 6 0.08130200875827853 1104 7 0.05758303149624311 1104 8 -0.002570901668427439 1104 9 0.05569408208035043 1104 10-11 0.10868145464262824 1104 12-13 0.05227044556460214 1104 14-15 0.11849864891377848 1104 16-17 0.24577937919745096 1104 18-19 0.2419081964439016 1104 20-21 0.21596160213258742 1104 22-23 0.08656747506808671 1104 24-25 0.05317365114895267 1104 26-27 0.11624915304732752 1104 28-29 0.03165975443931757 1104 30-31 -0.06824138723142426 1104 32-33 0.004921861857624776 1104 34-35 0.05052410677696173 1104 36-37 0.10933153355016145 1104 38-39 0.14237883648419114 1104 40-41 0.12769153664710586 1104 42-43 0.15765289355386614 1104 44-45 0.1476494616365187 1104 46-47 0.19952353183669658 1104 48-49 0.23141698876494843 1104 50-51 0.08386456668743136 1104 52-53 0.08833956498786222 1104 54-55 0.09582972021077296 1104 56-57 0.06550428779298301 1104 58-59 0.40436367856231215 1104 60-61 0.397769569759177 1104 62-63 0.37464713941390926 1104 64-65 0.30013145421071385 1104 66-67 0.23115778327962744 1104 68-69 0.3544198364196731 1104 70-71 0.3183972622253677 1104 72-73 0.4265662271215902 1104 74-75 0.36016424924752144 1104 76-77 0.3222369531939009 1104 78-79 0.27565177631565163 1104 80-81 0.390819856524228 1104 82-83 0.3789964742239107 1104 84-85 0.3920432267862033 1104 86-87 0.6574458973631039 1104 88-89 0.5133262982053886 1104 90-91 0.5607872732273407 1104 92-93 0.5831977674617832 1104 94-95 0.5165563852002286 1104 96-97 0.6142746232994973 1104 98-99 0.9537792406011825 1104 100-101 1.6663756469311117 1105 1 0.1689590751619363 1105 2 0.113809105809068 1105 3 0.0017650794777139822 1105 4 -0.006219317018697268 1105 5 -0.05372415347406445 1105 6 -0.03483554387424448 1105 7 0.018062552315754488 1105 8 0.05528351587127389 1105 9 0.1670767458202036 1105 10-11 0.1123938555932753 1105 12-13 0.10014370871493128 1105 14-15 0.03529825583603241 1105 16-17 0.0551415164891651 1105 18-19 0.09153326947241425 1105 20-21 0.018792650547887035 1105 22-23 0.04766206613677326 1105 24-25 0.054461340006838554 1105 26-27 0.005448826711500487 1105 28-29 0.04834759701158475 1105 30-31 0.10548588412078885 1105 32-33 0.13572101122905877 1105 34-35 0.02325930113207164 1105 36-37 0.05803703815119121 1105 38-39 0.12026230471938248 1105 40-41 0.1246864960252907 1105 42-43 0.15749419243098117 1105 44-45 0.09381988419037413 1105 46-47 0.03517380702892581 1105 48-49 0.043083896496611374 1105 50-51 -0.03012975268681828 1105 52-53 0.032747140668085706 1105 54-55 0.07687113990398586 1105 56-57 0.15715495436133153 1105 58-59 0.1521209469083047 1105 60-61 0.11138588976493224 1105 62-63 0.19538943526021768 1105 64-65 0.15207677783337914 1105 66-67 0.01398965415198461 1105 68-69 0.0997272226511754 1105 70-71 0.09019757343555312 1105 72-73 0.1689083957233919 1105 74-75 0.23581855164323784 1105 76-77 0.3634830559764559 1105 78-79 0.1246604792347128 1105 80-81 0.23612053074993966 1105 82-83 0.3674468611568642 1105 84-85 0.3354034695906485 1105 86-87 0.4523488616117497 1105 88-89 0.4130209255756263 1105 90-91 0.4321315934524108 1105 92-93 0.5279332624391238 1105 94-95 0.7435531123883905 1105 96-97 0.7540779048085824 1105 98-99 1.0004825113332458 1105 100-101 1.5330514598873606 1106 1 0.1392277148903034 1106 2 0.12374384173627107 1106 3 0.07473514200116682 1106 4 0.060517894310216036 1106 5 -0.03264306450525112 1106 6 -0.04249547664933573 1106 7 -0.014553409747911417 1106 8 0.031029741139029454 1106 9 0.0028181510680553856 1106 10-11 0.0011531427957791607 1106 12-13 0.0559657979952064 1106 14-15 0.0665150668980985 1106 16-17 0.11903300083185542 1106 18-19 0.0349626178759479 1106 20-21 0.0014727275405235218 1106 22-23 0.024721142617302405 1106 24-25 0.051840368679343385 1106 26-27 0.03119882545747288 1106 28-29 0.09557723698756604 1106 30-31 0.06313719831722864 1106 32-33 0.1676787236485282 1106 34-35 0.08195324674017002 1106 36-37 0.10450343230660764 1106 38-39 0.21576521930379755 1106 40-41 0.3555486343356975 1106 42-43 0.2782667568046975 1106 44-45 0.2870597036653493 1106 46-47 0.26273768597301483 1106 48-49 0.20499655062210564 1106 50-51 0.22888236211630186 1106 52-53 0.2319752762688907 1106 54-55 0.31470868380059613 1106 56-57 0.2408110853856158 1106 58-59 0.2969077100483304 1106 60-61 0.3877561183670295 1106 62-63 0.3807141846716249 1106 64-65 0.2974678104987092 1106 66-67 0.23591560323305316 1106 68-69 0.18736705224956296 1106 70-71 0.1879984906529657 1106 72-73 0.24817959685685054 1106 74-75 0.36069024198363664 1106 76-77 0.31559425433737687 1106 78-79 0.1941024536279592 1106 80-81 0.18898625375064526 1106 82-83 0.3836439748906386 1106 84-85 0.3745614002562334 1106 86-87 0.3825513708493631 1106 88-89 0.5029372113603934 1106 90-91 0.48384317933016163 1106 92-93 0.5235289521465809 1106 94-95 0.6849293922359152 1106 96-97 0.8094710259972011 1106 98-99 1.0630956871791533 1106 100-101 1.593569557306484 1107 1 0.035262856067085124 1107 2 0.04563500299626355 1107 3 -0.03956638109363553 1107 4 -0.044759935213157576 1107 5 -0.07979679772859072 1107 6 -0.033242476143705346 1107 7 0.03912060183719035 1107 8 -0.04374472196339951 1107 9 0.030818213162774555 1107 10-11 -0.07086863914530994 1107 12-13 0.021614665653608256 1107 14-15 0.03981816231993918 1107 16-17 0.018209635918921663 1107 18-19 0.04238127379135648 1107 20-21 0.006303974047703775 1107 22-23 -0.0045957710976480826 1107 24-25 0.010676225719549848 1107 26-27 -0.0030658732500725705 1107 28-29 0.04851836453899239 1107 30-31 0.05927479578376449 1107 32-33 0.006602688662582068 1107 34-35 -0.10591277946380018 1107 36-37 -0.08280568186783199 1107 38-39 -0.02123397568249885 1107 40-41 -0.14159406598631108 1107 42-43 -0.04995335345778784 1107 44-45 0.06626889412760306 1107 46-47 -0.10931896880695291 1107 48-49 -0.02444682564529188 1107 50-51 -0.1096178618682373 1107 52-53 -0.1297711599376612 1107 54-55 -0.1405521805579184 1107 56-57 -0.23176505826609173 1107 58-59 -0.10372438094704961 1107 60-61 -0.017516943168843113 1107 62-63 -0.03215938160561649 1107 64-65 -0.06919092800267634 1107 66-67 -0.1299245937846223 1107 68-69 -0.08595655660690937 1107 70-71 -0.071402151140326 1107 72-73 -0.11120502228074258 1107 74-75 -0.018833691095998972 1107 76-77 -0.02977420021372268 1107 78-79 -1.4519120798084373E-4 1107 80-81 0.06033303280875657 1107 82-83 0.1228200333838636 1107 84-85 0.11634510438375756 1107 86-87 0.005234295244605391 1107 88-89 0.08719808458958767 1107 90-91 0.2440144055234903 1107 92-93 0.3427275865062498 1107 94-95 0.39851738045566165 1107 96-97 0.4898244780201573 1107 98-99 0.6158332881939579 1107 100-101 1.2823575237930775 1108 1 0.11791560696221381 1108 2 0.006490523748027499 1108 3 0.018929725642379935 1108 4 0.03227288542089468 1108 5 -0.11052581818238849 1108 6 0.0028198357803717045 1108 7 0.015155604275101098 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-1.7651371471376827 2316 100-101 -1.6971490791605213 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 16.0 4 48.0 5 112.0 6 246.0 7 620.0 8 1417.0 9 2666.0 10 4464.0 11 6673.0 12 9296.0 13 12841.0 14 15671.0 15 20577.0 16 25541.0 17 33072.0 18 31731.0 19 24516.0 20 23116.0 21 24183.0 22 26284.0 23 29445.0 24 34364.0 25 40947.0 26 49356.0 27 60051.0 28 72801.0 29 87957.0 30 107118.0 31 130228.0 32 159191.0 33 200568.0 34 266469.0 35 369548.0 36 553869.0 37 854700.0 38 722584.0 39 71955.0 40 6.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.398483960223395 19.676166188701487 14.744657856559277 23.180691994515847 2 15.911313291372256 20.99759661405333 39.02868551086187 24.062404583712542 3 19.779974336393526 24.344198640633913 29.759941211294738 26.115885811677824 4 13.208068622166719 17.115558880501254 37.032638138239854 32.64373435909217 5 13.507901169339673 37.62156683856002 34.50558645227627 14.364945539824033 6 31.820464523084034 36.603835827863435 17.24676825439838 14.328931394654152 7 28.163230943986772 32.69100684002855 20.816434525895797 18.32932769008888 8 26.054303955670882 35.139873527705205 19.69657252423267 19.10924999239124 9 25.74433294749564 17.079699537485656 20.45511666926837 36.720850845750334 10-11 24.882923294877195 26.783898001400015 27.37223912512039 20.960939578602403 12-13 25.841135992569924 23.9611835497657 27.760433080722546 22.43724737694183 14-15 22.439922714647988 25.704141520946667 26.312498588698 25.543437175707346 16-17 21.948502191078866 28.677808746863903 27.00367403187106 22.370015030186167 18-19 21.740702342417702 27.464243743391425 29.18541857499242 21.60963533919846 20-21 22.608027191248848 26.466391867758794 29.24312241854456 21.6824585224478 22-23 22.076027697844903 26.534048458465502 29.128819228658415 22.261104615031176 24-25 22.154745424913603 26.598788976594406 29.321652627238453 21.924812971253534 26-27 21.93264784872998 26.737078070334334 29.301288253540942 22.02898582739474 28-29 21.72360717991916 27.319235530840995 29.13640344288964 21.8207538463502 30-31 22.08686404277399 26.764790199526196 29.03904814920628 22.109297608493534 32-33 21.808138033680784 26.852757266855363 29.05897818789805 22.280126511565804 34-35 22.403649018249386 26.94670864862244 28.88981209372424 21.759830239403925 36-37 22.346554063873494 26.646216864299266 29.03262312595985 21.97460594586739 38-39 21.97312147011407 27.02840601198297 29.04821725226284 21.950255265640124 40-41 22.299740815570605 26.92529553136981 28.895111922557394 21.879851730502196 42-43 22.02061747362432 26.98232146262951 28.57711105487266 22.41995000887351 44-45 22.202116907957027 26.949236058739316 28.633827941245954 22.2148190920577 46-47 22.49097069756654 27.183593975310703 28.193746801546794 22.131688525575967 48-49 22.364231642081773 27.23053363840889 28.158805865815516 22.24642885369382 50-51 22.207131517638086 27.410282918189644 28.220284361399344 22.162301202772927 52-53 22.52001103515443 27.402195544114598 27.876871631897092 22.200921788833877 54-55 22.11840357444753 27.259690858591956 28.090653204563687 22.531252362396827 56-57 22.314853779466944 27.397758900477655 27.855087329296474 22.432299990758935 58-59 22.459706536247058 27.21232997417429 27.901355199529533 22.426608290049124 60-61 22.206987960678916 27.271970840543425 28.29819716996183 22.222844028815828 62-63 22.069383282483155 27.298625124517596 28.30624044066311 22.325751152336146 64-65 21.892899653513822 27.4203316807752 28.076265985238535 22.61050268047244 66-67 21.93883737552847 27.38556522985628 27.857457393476327 22.81814000113892 68-69 22.099074601097676 27.209263168642618 28.050045124947136 22.64161710531257 70-71 22.122672884579984 27.1146714119163 27.86098054856722 22.90167515493649 72-73 22.04596082912888 27.10610438431398 28.017928198844842 22.830006587712294 74-75 21.895259562320128 27.60390389996057 27.64261056711649 22.858225970602813 76-77 21.99950145694909 27.15695387740016 27.84890952917152 22.99463513647923 78-79 22.01022883880706 27.27878378673992 27.859796873390835 22.851190501062185 80-81 22.150131908991938 27.267405550105966 27.66041420785696 22.92204833304514 82-83 22.135697139575182 27.223824437561056 27.820993957125534 22.819484465738228 84-85 21.995436217494646 27.3231135432958 27.69615134262682 22.985298896582734 86-87 21.95017077114897 27.25868969676435 27.728121470677376 23.063018061409306 88-89 21.92342205092799 27.207658189014218 27.522746748555537 23.346173011502263 90-91 21.88290710555136 27.158115806627798 27.609565832026632 23.349411255794205 92-93 22.056916458785082 27.156279296147535 27.665724690122772 23.121079554944615 94-95 21.799325199052536 26.94405347158739 27.814792874791205 23.44182845456887 96-97 21.649055641447365 26.763129481349562 28.013016883856086 23.57479799334699 98-99 21.630301709368986 26.914191856566553 27.614111743701663 23.841394690362797 100-101 21.76107442775353 26.324564835455057 28.195039720622866 23.71932101616855 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1061.0 1 729.0 2 500.5 3 650.0 4 589.0 5 561.5 6 1031.5 7 2137.0 8 3353.0 9 3840.0 10 3364.5 11 2874.5 12 2927.5 13 3206.0 14 3595.5 15 3926.0 16 4259.5 17 4643.5 18 5093.5 19 5692.5 20 6499.0 21 7787.5 22 9571.5 23 11731.0 24 14338.5 25 18066.0 26 23466.5 27 29935.0 28 36102.5 29 43082.5 30 50780.5 31 59314.0 32 69915.0 33 76993.0 34 82067.5 35 89114.0 36 98889.0 37 107817.5 38 112946.0 39 117124.5 40 121249.0 41 126036.0 42 130487.0 43 134137.5 44 138210.0 45 144561.0 46 148427.0 47 154814.0 48 169542.0 49 168874.0 50 153966.5 51 145582.5 52 140551.0 53 137426.0 54 133760.0 55 124679.0 56 113444.0 57 101641.0 58 92063.0 59 81481.5 60 66877.5 61 54489.5 62 44482.5 63 34030.0 64 24403.5 65 17819.5 66 13301.0 67 10349.0 68 7690.0 69 5480.0 70 4083.5 71 2928.0 72 2160.0 73 1829.5 74 1427.0 75 1010.0 76 733.0 77 445.5 78 268.5 79 181.5 80 106.5 81 59.5 82 43.5 83 29.5 84 18.0 85 12.0 86 10.0 87 5.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2319689601919567 2 0.0 3 0.0 4 0.0 5 0.02906054902838607 6 0.003387124802294998 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0028839649584758136 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.9453259150391287E-4 26-27 0.001325396661767608 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006860154943778637 36-37 0.017181067837728253 38-39 0.013609860165743308 40-41 0.007829658057479018 42-43 0.008075101883732278 44-45 0.0036693852024862477 46-47 4.1725450463054323E-4 48-49 0.001718106783772825 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0011413137920776623 58-59 4.417988872558693E-4 60-61 0.0025035270277832594 62-63 6.99514904821793E-4 64-65 0.0015585682967082055 66-67 0.005596119238574344 68-69 2.2089944362793464E-4 70-71 4.908876525065214E-5 72-73 0.0 74-75 4.5407107856853233E-4 76-77 0.05858744132665333 78-79 0.14125292200875156 80-81 0.0010185918789510319 82-83 7.854202440104343E-4 84-85 0.0 86-87 0.10631399334159988 88-89 0.3544454294923338 90-91 0.39259967228340314 92-93 4.2952669594320623E-4 94-95 3.31349165441902E-4 96-97 1.2272191312663036E-4 98-99 0.0 100-101 0.0011290416007649993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4074252.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.99972482593721 #Duplication Level Percentage of deduplicated Percentage of total 1 71.11716737223472 36.980731337563256 2 15.508668083148242 16.12892945481007 3 5.7997692478895875 9.04759214832574 4 2.6831956123107363 5.581017339772815 5 1.4743983971254209 3.8334155467162385 6 0.8431875465687667 2.6307312238939793 7 0.5343447439707585 1.945004575406572 8 0.374532670127952 1.5580476627981614 9 0.2691635580351703 1.2596787855899132 >10 1.2766591404989174 11.661890756189862 >50 0.07925325965848204 2.8098878284928217 >100 0.03550240839279633 3.3137393223010845 >500 0.0024569764338863943 0.9380931965243129 >1k 0.0015119854262949353 1.3798551678314084 >5k 9.449908914342881E-5 0.3810013013935122 >10k+ 9.449908914342881E-5 0.5503843523902796 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11997 0.2944589583560369 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10404 0.25535975683389245 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8785 0.21562240136348954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6722 0.16498734000744186 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.4544382625326074E-5 2 4.908876525065215E-5 0.0 0.0 0.0 2.4544382625326074E-5 3 4.908876525065215E-5 0.0 0.0 0.0 2.4544382625326074E-5 4 4.908876525065215E-5 0.0 0.0 0.0 4.908876525065215E-5 5 7.363314787597822E-5 0.0 0.0 0.0 4.908876525065215E-5 6 7.363314787597822E-5 0.0 0.0 0.0 7.363314787597822E-5 7 1.4726629575195644E-4 0.0 0.0 0.0 9.81775305013043E-5 8 1.4726629575195644E-4 0.0 0.0 2.4544382625326074E-5 9.81775305013043E-5 9 1.4726629575195644E-4 0.0 0.0 2.4544382625326074E-5 9.81775305013043E-5 10-11 1.4726629575195644E-4 0.0 0.0 2.4544382625326074E-5 9.81775305013043E-5 12-13 1.718106783772825E-4 0.0 0.0 4.908876525065215E-5 1.349941044392934E-4 14-15 1.718106783772825E-4 0.0 0.0 6.136095656331518E-5 1.9635506100260856E-4 16-17 1.718106783772825E-4 0.0 0.0 1.1044972181396733E-4 2.577160175659237E-4 18-19 1.718106783772825E-4 0.0 0.0 1.2272191312663036E-4 2.9453259150391287E-4 20-21 1.718106783772825E-4 0.0 0.0 2.0862725231527162E-4 2.9453259150391287E-4 22-23 1.963550610026086E-4 0.0 0.0 3.43621356754565E-4 3.31349165441902E-4 24-25 2.6998820887858677E-4 0.0 0.0 4.908876525065214E-4 3.681657393798911E-4 26-27 2.6998820887858677E-4 0.0 0.0 8.713255831990756E-4 3.681657393798911E-4 28-29 3.068047828165759E-4 0.0 0.0 0.0027366986627238568 3.681657393798911E-4 30-31 3.43621356754565E-4 0.0 0.0 0.008774616788554072 4.049823133178802E-4 32-33 3.43621356754565E-4 0.0 0.0 0.021574512327661617 4.663432698811954E-4 34-35 3.43621356754565E-4 0.0 0.0 0.04478122609990742 4.663432698811954E-4 36-37 3.43621356754565E-4 0.0 0.0 0.08304591861279076 4.908876525065214E-4 38-39 3.681657393798911E-4 0.0 0.0 0.14164563213075676 5.031598438191844E-4 40-41 3.681657393798911E-4 0.0 0.0 0.2033870266247645 5.154320351318475E-4 42-43 3.681657393798911E-4 0.0 0.0 0.2702950136614034 5.277042264445105E-4 44-45 3.681657393798911E-4 0.0 0.0 0.35292367776956357 5.522486090698366E-4 46-47 4.0498231331788023E-4 0.0 0.0 0.45499149291698204 5.890651830078257E-4 48-49 4.1725450463054323E-4 0.0 0.0 0.5633672143991093 6.136095656331518E-4 50-51 4.1725450463054323E-4 0.0 0.0 0.6796830436605297 6.136095656331518E-4 52-53 4.1725450463054323E-4 0.0 0.0 0.7983305892713559 6.50426139571141E-4 54-55 4.1725450463054323E-4 0.0 0.0 0.9328583504407679 6.99514904821793E-4 56-57 4.417988872558693E-4 0.0 0.0 1.0951212639767987 7.11787096134456E-4 58-59 4.417988872558693E-4 0.0 0.0 1.2738043694891725 7.11787096134456E-4 60-61 4.417988872558693E-4 0.0 0.0 1.4661464239325404 7.363314787597822E-4 62-63 4.417988872558693E-4 0.0 0.0 1.6586971056282234 7.363314787597822E-4 64-65 4.663432698811954E-4 0.0 0.0 1.863961777523825 7.486036700724453E-4 66-67 5.399764177571735E-4 0.0 0.0 2.105797579531163 7.608758613851083E-4 68-69 5.645208003824996E-4 0.0 0.0 2.3788783806205407 7.608758613851083E-4 70-71 5.645208003824996E-4 0.0 0.0 2.6691525217389596 8.467812005737495E-4 72-73 5.767929916951626E-4 0.0 0.0 2.9672317765322322 9.081421571370647E-4 74-75 6.013373743204888E-4 0.0 0.0 3.2731406893829837 9.081421571370647E-4 76-77 6.136095656331518E-4 0.0 0.0 3.6069688374700437 9.204143484497277E-4 78-79 6.136095656331518E-4 0.0 0.0 3.98889170331143 9.326865397623908E-4 80-81 6.8724271350913E-4 0.0 0.0 4.40327451517481 9.449587310750538E-4 82-83 7.11787096134456E-4 0.0 0.0 4.820295848170413 0.0010063196876383689 84-85 7.486036700724453E-4 0.0 0.0 5.242986933552466 0.0010063196876383689 86-87 7.854202440104344E-4 0.0 0.0 5.68084644739697 0.0010063196876383689 88-89 7.854202440104344E-4 0.0 0.0 6.163560820489257 0.0010063196876383689 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14745 0.0 21.934238 1 GTATCAA 25245 0.0 21.835669 1 TACATGG 14325 0.0 21.43289 2 GAGTACT 13620 0.0 20.745174 12-13 GGTATCA 19360 0.0 20.366087 1 ACATGGG 14315 0.0 20.055574 3 GTACTTT 14520 0.0 19.410301 14-15 TCAACGC 28770 0.0 18.902351 4 ATCAACG 28810 0.0 18.859636 3 CAACGCA 29020 0.0 18.72316 5 TATCAAC 29155 0.0 18.717848 2 AGAGTAC 21675 0.0 18.541153 10-11 AGTACTT 14665 0.0 18.441881 12-13 AACGCAG 29615 0.0 18.374977 6 GAGTACA 12830 0.0 17.312057 1 AGTACAT 12020 0.0 16.975988 2 GTATAGG 1150 0.0 16.956821 1 TAGCCCT 3345 0.0 16.881924 4 GTGTAGC 2455 0.0 16.854902 1 ACTTTTT 17065 0.0 16.709341 16-17 >>END_MODULE