FastQCFastQC Report
Thu 26 May 2016
SRR938709_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938709_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3390459
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179660.5298987541214921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135610.3999753425716105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92000.27134969041064944No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT84450.24908131907803635No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49700.14658782188488342No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37990.11204972542065837No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36090.10644576442304715No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34170.10078281436230316No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34040.10039938545194028No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG191050.025.9264811
TACATGG193300.025.0752322
ACATGGG195650.023.926453
GTATCAA304350.023.7503951
TACCTGG26700.023.4241492
GAGTACT148900.022.1008612-13
AGTACTT152750.021.1563412-13
GTACTTT157750.020.7410614-15
TCAACGC351600.020.4561564
CATGGGG73950.020.4387194
ATCAACG352350.020.4126153
TATCAAC352950.020.4047622
CAACGCA352700.020.3923575
GGTATCA228650.019.9838981
AACGCAG360200.019.9539056
CATGGGA113150.019.7646264
GTGTAGC16800.019.4676781
ACCGACG1559.836658E-418.3289958
ACGCAGA390900.018.3149287
CGCAGAG392200.018.2188
Produced by FastQC (version 0.11.5)