##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938709_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3390459 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.549098219444623 33.0 31.0 34.0 30.0 34.0 2 31.60331005329957 33.0 31.0 34.0 28.0 34.0 3 31.60873232798273 33.0 31.0 34.0 28.0 34.0 4 35.1595580421412 37.0 35.0 37.0 33.0 37.0 5 35.19813128546902 37.0 35.0 37.0 33.0 37.0 6 35.29063852416443 37.0 35.0 37.0 33.0 37.0 7 35.20326716825067 37.0 35.0 37.0 32.0 37.0 8 35.17448728918415 37.0 35.0 37.0 32.0 37.0 9 36.7304928329763 39.0 37.0 39.0 32.0 39.0 10-11 36.623790908546596 39.0 37.0 39.0 32.0 39.0 12-13 36.50114468277009 39.0 37.0 39.0 32.0 39.0 14-15 37.6248463703587 40.0 37.0 41.0 32.0 41.0 16-17 37.56705316300831 40.0 37.0 41.0 32.0 41.0 18-19 37.52270916710687 40.0 37.0 41.0 32.0 41.0 20-21 37.427247461184464 40.0 37.0 41.0 32.0 41.0 22-23 37.33802296385238 40.0 37.0 41.0 31.5 41.0 24-25 37.34032810896696 40.0 37.0 41.0 31.0 41.0 26-27 37.24140551471055 40.0 37.0 41.0 31.0 41.0 28-29 37.115933122919344 40.0 36.5 41.0 31.0 41.0 30-31 36.898922977685324 39.5 36.0 41.0 30.0 41.0 32-33 36.67573136852562 39.0 35.5 41.0 30.0 41.0 34-35 36.33450500359981 39.0 35.0 41.0 30.0 41.0 36-37 36.06954235399986 39.0 35.0 41.0 29.0 41.0 38-39 35.893114177165984 39.0 35.0 41.0 28.5 41.0 40-41 35.706124008578186 39.0 35.0 41.0 27.0 41.0 42-43 35.430247792408046 38.5 35.0 40.0 26.5 41.0 44-45 35.3501608484279 39.0 35.0 41.0 26.0 41.0 46-47 35.115810868086 39.0 35.0 41.0 24.5 41.0 48-49 34.84969557219244 39.0 34.5 41.0 24.0 41.0 50-51 33.69858269927464 38.0 33.0 40.0 22.0 40.5 52-53 33.79486980966294 38.0 33.5 39.5 22.0 40.5 54-55 34.312922232653456 38.0 34.0 40.0 22.0 41.0 56-57 34.227893184964046 38.0 34.0 40.0 22.0 41.0 58-59 34.016100032473474 38.0 34.0 40.0 21.0 41.0 60-61 33.727109662732985 38.0 33.5 40.0 20.0 41.0 62-63 33.38202600296892 37.0 33.0 40.0 18.5 41.0 64-65 32.999849872834325 37.0 33.0 40.0 17.0 41.0 66-67 32.60562434171892 36.0 32.0 39.0 13.0 41.0 68-69 32.21051028784008 36.0 32.0 39.0 11.0 41.0 70-71 31.805726599259863 35.0 31.0 38.5 8.0 40.0 72-73 31.399227951141718 35.0 31.0 37.5 7.0 40.0 74-75 30.95428082156428 35.0 31.0 37.0 3.5 39.0 76-77 30.52253308475342 35.0 30.0 36.5 2.0 39.0 78-79 30.112100161069637 35.0 30.0 36.0 2.0 39.0 80-81 29.679981530524334 34.0 29.5 36.0 2.0 37.5 82-83 29.227602221410137 34.0 29.0 35.0 2.0 37.0 84-85 28.834035893075246 34.0 29.0 35.0 2.0 36.5 86-87 28.465961835845825 34.0 29.0 35.0 2.0 36.0 88-89 28.100425635585033 34.0 28.0 35.0 2.0 36.0 90-91 27.7044568301814 34.0 27.0 35.0 2.0 35.5 92-93 27.28360732278432 34.0 26.0 35.0 2.0 35.0 94-95 26.80573706981857 33.5 25.0 35.0 2.0 35.0 96-97 26.244419855836625 33.0 24.5 35.0 2.0 35.0 98-99 25.57316398753089 33.0 21.5 35.0 2.0 35.0 100-101 23.388292853563485 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.188521540373781 1101 2 0.08152292075260448 1101 3 0.215983849241983 1101 4 0.16956502982237964 1101 5 0.03670665357167735 1101 6 -0.05399677798016711 1101 7 0.1391371521160636 1101 8 0.1557099380844278 1101 9 0.24061382323758096 1101 10-11 0.20693202781718867 1101 12-13 0.10284128760385158 1101 14-15 0.29867544323311535 1101 16-17 0.24579480417009592 1101 18-19 0.260192994765994 1101 20-21 0.3954081132834588 1101 22-23 0.2933301508056658 1101 24-25 0.24663143081600225 1101 26-27 0.3118054589216399 1101 28-29 0.25742696323842296 1101 30-31 0.17382948083012906 1101 32-33 0.11580617217146028 1101 34-35 0.15138062506552075 1101 36-37 0.21408748985925286 1101 38-39 0.20296437289433555 1101 40-41 0.27241727395583837 1101 42-43 0.1445308915302661 1101 44-45 0.24500232221143392 1101 46-47 0.28184184658559985 1101 48-49 0.2697497524272876 1101 50-51 0.244889752464168 1101 52-53 0.16170867040347758 1101 54-55 0.1322965839953696 1101 56-57 0.1912139267787083 1101 58-59 0.036882723339566326 1101 60-61 0.022066800856975988 1101 62-63 -0.02220425677325011 1101 64-65 0.0408550328130346 1101 66-67 -0.11283795812236264 1101 68-69 -0.10751242087533086 1101 70-71 -0.2593593605971982 1101 72-73 -0.21040185401331613 1101 74-75 -0.2217651591976555 1101 76-77 -0.38001523261993597 1101 78-79 -0.4642877858994119 1101 80-81 -0.524513556593746 1101 82-83 -0.7086562673977816 1101 84-85 -0.5696857295105353 1101 86-87 -0.5610302670235079 1101 88-89 -0.6736903642701897 1101 90-91 -0.6751891844750091 1101 92-93 -0.6971594323863606 1101 94-95 -0.8462086149311823 1101 96-97 -0.9457917411830614 1101 98-99 -1.0902867061809047 1101 100-101 -0.849661907194875 1102 1 0.2627376649633675 1102 2 0.30614004191562927 1102 3 0.19469781733126013 1102 4 0.18860110468402524 1102 5 0.17040883324030176 1102 6 0.1657729043172793 1102 7 0.16333601239151818 1102 8 0.16641773019410522 1102 9 0.24417805068330978 1102 10-11 0.2625193418760574 1102 12-13 0.34159094273728385 1102 14-15 0.24236383943149775 1102 16-17 0.2935053098829812 1102 18-19 0.28561966396214444 1102 20-21 0.33717281159446344 1102 22-23 0.36821712131306583 1102 24-25 0.3522487405713761 1102 26-27 0.3106092685989523 1102 28-29 0.23618262310736782 1102 30-31 0.30965346429220375 1102 32-33 0.33831016366969635 1102 34-35 0.4014297318261342 1102 36-37 0.2816236959520708 1102 38-39 0.29846568996283906 1102 40-41 0.2106471249343116 1102 42-43 0.2724886994492479 1102 44-45 0.40977634996752954 1102 46-47 0.3268553978130413 1102 48-49 0.3408471080492177 1102 50-51 0.3718163184102181 1102 52-53 0.429161297069534 1102 54-55 0.301853134557426 1102 56-57 0.23457785460323777 1102 58-59 0.25934796106944447 1102 60-61 0.24929343569238682 1102 62-63 0.2927823151159714 1102 64-65 0.1888241954462373 1102 66-67 0.15017645268324742 1102 68-69 0.134615387653632 1102 70-71 0.1253006107613892 1102 72-73 0.06500648758842331 1102 74-75 -0.017563579163148546 1102 76-77 0.1270888787491664 1102 78-79 -0.05338018103899245 1102 80-81 -0.008735596379455757 1102 82-83 -0.021057389326369247 1102 84-85 -0.10056842563118451 1102 86-87 -0.22443140406187112 1102 88-89 -0.18544101049277728 1102 90-91 -0.20992711106725537 1102 92-93 -0.2944992450139914 1102 94-95 -0.3071471948252231 1102 96-97 -0.20739268873368744 1102 98-99 -0.1931541772470915 1102 100-101 -0.07914311595429169 1103 1 0.09763355187710587 1103 2 0.12410731759493743 1103 3 0.12317335294275722 1103 4 0.1560063396877922 1103 5 0.05847243491071907 1103 6 0.20195314389171415 1103 7 0.11832581389165142 1103 8 0.1280637422166535 1103 9 0.2029878656689661 1103 10-11 0.22599476654459494 1103 12-13 0.2943044423676895 1103 14-15 0.3206167189512925 1103 16-17 0.319967909362596 1103 18-19 0.3565839122741252 1103 20-21 0.34407204220048726 1103 22-23 0.32285873614369365 1103 24-25 0.3503448769508921 1103 26-27 0.3206238415520062 1103 28-29 0.23023970243232128 1103 30-31 0.14376312324255736 1103 32-33 0.09877034220900072 1103 34-35 0.22509768744821912 1103 36-37 0.3806135557660255 1103 38-39 0.20313538894801297 1103 40-41 0.30390801893647534 1103 42-43 0.2922382874474536 1103 44-45 0.36352391128551176 1103 46-47 0.436323269558585 1103 48-49 0.5049035437216531 1103 50-51 0.533565690757321 1103 52-53 0.4781446286699591 1103 54-55 0.44029724305560336 1103 56-57 0.41991603715226233 1103 58-59 0.38042757171652397 1103 60-61 0.5044414180067989 1103 62-63 0.37748936978590564 1103 64-65 0.0848611113934723 1103 66-67 0.17978506427710528 1103 68-69 0.022974134258639367 1103 70-71 0.1077189947974837 1103 72-73 0.17008647379115516 1103 74-75 0.13521018724746625 1103 76-77 0.1331986264538827 1103 78-79 0.12668715675003384 1103 80-81 0.2292025205573509 1103 82-83 0.27276801176908805 1103 84-85 0.35135196940641933 1103 86-87 0.3215210760449594 1103 88-89 0.3158986486061153 1103 90-91 0.22632768033890116 1103 92-93 0.14659421791538207 1103 94-95 0.1135053954727141 1103 96-97 0.07820060759788205 1103 98-99 0.20808275417714128 1103 100-101 0.13733863356292275 1104 1 0.15037630435628913 1104 2 0.2402233433298271 1104 3 0.16571978005951493 1104 4 0.20643713784070172 1104 5 0.1692191529202347 1104 6 0.16592836822903934 1104 7 0.20362813561991544 1104 8 0.2491984492546493 1104 9 0.3225552907666298 1104 10-11 0.26363769222959377 1104 12-13 0.2998515158724331 1104 14-15 0.3339591443487109 1104 16-17 0.32611940468436984 1104 18-19 0.34184570304134354 1104 20-21 0.3555534224840997 1104 22-23 0.33896547845362335 1104 24-25 0.4048532060542911 1104 26-27 0.36126562234181847 1104 28-29 0.2782729788781708 1104 30-31 0.2680719077606426 1104 32-33 0.24121214458141083 1104 34-35 0.26141795216571495 1104 36-37 0.38006399880885056 1104 38-39 0.41235299424585037 1104 40-41 0.20483679744485528 1104 42-43 0.3340941803512507 1104 44-45 0.3967265984282804 1104 46-47 0.2688619102253611 1104 48-49 0.3917934582887028 1104 50-51 0.36657059189702323 1104 52-53 0.4018572210999878 1104 54-55 0.38691137268273224 1104 56-57 0.3923201935105709 1104 58-59 0.25861277658954407 1104 60-61 0.2803498947003149 1104 62-63 0.26825216210932723 1104 64-65 0.2655696093062403 1104 66-67 0.18113125173252342 1104 68-69 0.17355419389783577 1104 70-71 0.19209406405982676 1104 72-73 0.189122400100743 1104 74-75 0.0500044240391162 1104 76-77 0.03773495116955772 1104 78-79 0.15686192131256504 1104 80-81 0.09749669939571959 1104 82-83 0.021217443627111976 1104 84-85 0.08120257711649614 1104 86-87 0.022182942460290178 1104 88-89 0.08071670256043362 1104 90-91 0.18197062201101488 1104 92-93 0.2240343325575047 1104 94-95 0.2986295154094769 1104 96-97 0.4349585412927901 1104 98-99 0.34316412675786623 1104 100-101 0.6057684485084742 1105 1 0.08140120054088484 1105 2 0.12782272587050514 1105 3 0.07961023383984056 1105 4 0.1361212099788247 1105 5 0.2138201700489475 1105 6 0.06567644010078766 1105 7 0.1349672930346273 1105 8 0.10642181210222645 1105 9 0.11421074660473352 1105 10-11 0.1371833501526254 1105 12-13 0.18483808167475502 1105 14-15 0.17886983991774486 1105 16-17 0.07410329044973452 1105 18-19 0.07730886108117829 1105 20-21 0.24764716130123787 1105 22-23 0.3127478929644596 1105 24-25 0.17043876396510171 1105 26-27 0.07149564437680311 1105 28-29 0.1526043346303041 1105 30-31 0.06702603815211461 1105 32-33 0.14675646096433326 1105 34-35 0.20892710745193455 1105 36-37 0.21089948793304103 1105 38-39 0.22862725058526934 1105 40-41 0.21050428525436615 1105 42-43 0.13335688823939051 1105 44-45 0.16755806329018696 1105 46-47 0.1722090832258587 1105 48-49 0.15902412137721456 1105 50-51 0.17810443553116073 1105 52-53 0.15203144766918086 1105 54-55 0.17865485925847224 1105 56-57 0.12092205594918681 1105 58-59 0.1396422108101305 1105 60-61 0.22424580665489913 1105 62-63 0.23780489435608132 1105 64-65 0.0844472840846251 1105 66-67 0.02906439563194141 1105 68-69 -0.027219259289772424 1105 70-71 -0.018502491651410935 1105 72-73 0.037027528117217656 1105 74-75 -0.05591955678691818 1105 76-77 0.03775119680136285 1105 78-79 -0.07156960813803437 1105 80-81 0.007755068491821504 1105 82-83 0.09587762349803342 1105 84-85 -0.0755236425455692 1105 86-87 -0.16811516622594525 1105 88-89 -0.269246026469407 1105 90-91 -0.22563568125832134 1105 92-93 -0.1114343391513053 1105 94-95 -0.05684965733251701 1105 96-97 -0.14529035968798 1105 98-99 -0.13703620739195443 1105 100-101 0.06395107339799821 1106 1 0.14055495024146225 1106 2 0.17430935124034974 1106 3 0.16847922816989325 1106 4 0.23684397127222212 1106 5 0.10427653606244291 1106 6 0.07500449022437294 1106 7 0.11077827509874538 1106 8 0.2050080048323295 1106 9 0.2577564119773612 1106 10-11 0.2880422731968082 1106 12-13 0.2426017102281719 1106 14-15 0.30038406564248277 1106 16-17 0.2740915142983198 1106 18-19 0.3271425898523219 1106 20-21 0.34395520401120194 1106 22-23 0.27374246924380685 1106 24-25 0.1576404961299147 1106 26-27 -0.009678036460044837 1106 28-29 0.2903494425092177 1106 30-31 0.33023225596006256 1106 32-33 0.33616983465775263 1106 34-35 0.3115741989432692 1106 36-37 0.3009630925327045 1106 38-39 0.33412711475806844 1106 40-41 0.597554800929359 1106 42-43 0.511516543412263 1106 44-45 0.49138268963156406 1106 46-47 0.496080412713205 1106 48-49 0.5641646791118049 1106 50-51 0.6242677520165714 1106 52-53 0.4364245677203513 1106 54-55 0.4595563504136493 1106 56-57 0.4322040104691567 1106 58-59 0.45387569709961184 1106 60-61 0.5297290771597858 1106 62-63 0.3887425035029324 1106 64-65 0.35400098132780045 1106 66-67 0.2825409697889114 1106 68-69 0.44847355980044057 1106 70-71 0.4053570616845228 1106 72-73 0.31794735259903106 1106 74-75 0.22764508424788232 1106 76-77 0.261371613971523 1106 78-79 0.5310865831066636 1106 80-81 0.3182569868001224 1106 82-83 0.3674681934771442 1106 84-85 0.37218850549136206 1106 86-87 0.48203384223548795 1106 88-89 0.5944426120870538 1106 90-91 0.619502039494332 1106 92-93 0.6202673281108595 1106 94-95 0.5972522572288277 1106 96-97 0.6270704776499123 1106 98-99 0.8588211298863868 1106 100-101 1.0794353743161373 1107 1 0.08640266136660912 1107 2 0.05543672672997957 1107 3 0.16155256926418815 1107 4 0.14887833528224093 1107 5 0.06815630379040982 1107 6 0.10461717295935813 1107 7 -0.04476968190621733 1107 8 0.10086693977075356 1107 9 0.027451855109170253 1107 10-11 -0.004112890366343436 1107 12-13 0.11316347513366054 1107 14-15 0.14559315049276478 1107 16-17 0.18888541376427526 1107 18-19 0.22942493923135743 1107 20-21 0.14817179054505658 1107 22-23 0.14829878059412493 1107 24-25 0.08787509992929898 1107 26-27 0.14993990145573122 1107 28-29 8.591950458836095E-4 1107 30-31 0.07912305608743964 1107 32-33 0.0495282171061433 1107 34-35 0.04550382998433378 1107 36-37 0.19417294651872652 1107 38-39 0.25359577595726535 1107 40-41 0.23378048484241276 1107 42-43 0.1703793971146439 1107 44-45 0.20183062418890074 1107 46-47 0.21595104303908386 1107 48-49 0.23054191930663137 1107 50-51 0.3030372566548749 1107 52-53 0.15476845491841829 1107 54-55 0.10090861979908539 1107 56-57 0.015215724997993618 1107 58-59 -0.01045654691478859 1107 60-61 -0.07443147731399335 1107 62-63 0.024459157031650136 1107 64-65 -0.079932564996966 1107 66-67 0.0017202480194171699 1107 68-69 0.01588508743973449 1107 70-71 0.06047172729318717 1107 72-73 -0.03148862903219651 1107 74-75 -0.04886273500870075 1107 76-77 -0.20052854281383503 1107 78-79 -0.19946646376711286 1107 80-81 -0.30219632474683067 1107 82-83 -0.21667033970097194 1107 84-85 -0.3262879271109469 1107 86-87 -0.22494562949703223 1107 88-89 -0.2108579859942843 1107 90-91 -0.1360830256994916 1107 92-93 0.004903624356888514 1107 94-95 -0.1011854575679898 1107 96-97 -0.12644553412030035 1107 98-99 9.324857752375237E-4 1107 100-101 0.13304430881953166 1108 1 0.14187363441270762 1108 2 0.0618426374160137 1108 3 0.039680385912294724 1108 4 0.14907662730894344 1108 5 0.22694315081241 1108 6 0.18805743011564857 1108 7 0.14301724766725954 1108 8 0.23299370178157375 1108 9 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-2.5769253703333526 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11999.0 3 3932.0 4 3901.0 5 4456.0 6 5059.0 7 6112.0 8 7348.0 9 9051.0 10 11326.0 11 14234.0 12 17772.0 13 22703.0 14 24753.0 15 24353.0 16 29794.0 17 37475.0 18 31885.0 19 23206.0 20 22915.0 21 23893.0 22 26127.0 23 29504.0 24 33397.0 25 37668.0 26 43536.0 27 51091.0 28 59700.0 29 70714.0 30 84281.0 31 100725.0 32 124256.0 33 156500.0 34 207508.0 35 291506.0 36 422206.0 37 625198.0 38 586325.0 39 103987.0 40 63.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.66009350142911 18.900375925966955 14.134048257372653 22.305482315231277 2 15.251581285841864 20.800879763231507 40.54498084743636 23.402558103490268 3 19.63505164438716 25.585145120595083 29.666334365856994 25.113468869160766 4 12.4384148096753 16.618339373629862 37.72986219266455 33.21338362403029 5 13.251937179825275 38.137850363102174 33.85619494441766 14.754017512654901 6 30.930527143215013 36.24984841014329 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21.33679765103321 27.02771429562358 30.238357553302258 21.397130500040955 30-31 21.531625296813882 26.643088307580566 30.09255590563521 21.732730489970344 32-33 21.344334541874687 26.79852909322806 30.03917775119236 21.817958613704892 34-35 22.194050636375266 26.607445690479555 29.694884517617265 21.50361915552791 36-37 22.08049844620822 26.375133878587903 29.839899879264774 21.7044677959391 38-39 21.736957868240868 26.797429580036447 29.96035217426022 21.50526037746247 40-41 21.841129596851626 26.695903734798236 29.965249941090928 21.49771672725921 42-43 21.616759849039518 26.82495274707008 29.470553846709986 22.087733557180417 44-45 21.73459140109637 26.949812910771758 29.275619311721446 22.03997637641043 46-47 22.0279365408621 27.148702258407447 28.82766539861984 21.995695802110614 48-49 22.17937212288191 27.194626850454473 28.582875227175997 22.04312579948762 50-51 21.790416695066696 27.402879318162288 28.759397939195296 22.047306047575724 52-53 22.169673132842323 27.544562791856258 28.42671276550186 21.859051309799554 54-55 21.615911431674128 27.238022930647528 28.70601229019172 22.44005334748663 56-57 21.961581239300717 27.66216247445356 28.295334890167357 22.080921396078367 58-59 22.313104649666236 27.091207913806198 28.372453160547618 22.22323427597995 60-61 21.9934173122024 27.18185156231493 28.973361286807382 21.85136983867529 62-63 21.915821641647764 27.162339617095814 28.94373650389565 21.978102237360773 64-65 21.584679126093302 27.29891360407668 28.75629423153761 22.360113038292404 66-67 21.654310243638704 27.49393541303809 28.324451845519615 22.52730249780359 68-69 21.680433070317605 27.323171402323354 28.611461327734872 22.38493419962417 70-71 21.738131572737803 27.350652338113076 28.413377111072375 22.497838978076746 72-73 21.813775617575416 27.149783712998975 28.55692376275951 22.4795169066661 74-75 21.601238411014105 27.69677602348073 28.27078296657292 22.431202598932245 76-77 21.815955301567737 27.291098391964713 28.35098226019857 22.54196404626898 78-79 21.784102247491923 27.4194222123208 28.32410407240891 22.472371467778366 80-81 21.83290969128768 27.696798012736366 28.160683460361756 22.309608835614195 82-83 21.940515318590688 27.47103064458899 28.17822144491582 22.410232591904506 84-85 21.765357480586065 27.44751455535985 28.067225695597237 22.719902268456853 86-87 21.868274141082907 27.395898557712112 28.20157181554148 22.5342554856635 88-89 21.881026437416924 27.29994858032805 28.00896213763579 22.810062844619235 90-91 21.85792398313371 27.327021069824635 28.191852740327917 22.62320220671374 92-93 22.125263466914127 27.344038627143057 28.143905135757986 22.38679277018483 94-95 21.854522125311533 27.198092855335886 28.073327139598668 22.874057879753913 96-97 21.944675885376423 26.96512617408725 28.175589418550228 22.9146085219861 98-99 21.91791154703041 27.112339912727222 27.804985686209825 23.164762854032535 100-101 22.157581580266875 26.581197391707374 28.350587411111917 22.910633616913838 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4759.0 1 2870.0 2 1333.0 3 1728.0 4 1477.0 5 1413.0 6 2465.5 7 4795.0 8 7262.5 9 8004.0 10 6715.5 11 5468.0 12 5485.0 13 5882.5 14 6078.0 15 6381.5 16 6833.5 17 7066.5 18 7329.0 19 7784.0 20 8287.5 21 9208.5 22 10550.5 23 12162.5 24 14317.0 25 17262.0 26 21367.0 27 26324.0 28 30338.0 29 34731.0 30 40592.5 31 47028.5 32 55142.5 33 62279.0 34 69002.0 35 75481.0 36 82448.5 37 90271.5 38 93884.5 39 94519.5 40 95618.0 41 99269.0 42 103261.0 43 107755.5 44 112717.0 45 116418.0 46 117406.5 47 123524.5 48 147670.0 49 149516.0 50 128777.0 51 118991.5 52 111978.0 53 110089.5 54 107755.0 55 98835.5 56 88900.0 57 79290.5 58 70717.0 59 61793.5 60 52112.5 61 42916.0 62 35213.5 63 27939.0 64 20939.5 65 16481.0 66 12544.0 67 10029.0 68 7434.0 69 4731.0 70 3483.0 71 2728.5 72 2182.5 73 1842.0 74 1490.0 75 1293.5 76 986.0 77 582.0 78 365.5 79 260.0 80 165.0 81 104.0 82 86.5 83 75.0 84 54.5 85 36.5 86 28.0 87 20.5 88 18.5 89 14.0 90 10.5 91 9.0 92 10.0 93 11.5 94 7.5 95 6.0 96 6.0 97 4.5 98 4.0 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16174801111000014 2 0.0939105885073378 3 0.08748078062586806 4 0.10275894797725027 5 0.12452591227323498 6 0.040702453561597414 7 0.04480219344932353 8 0.048253053642589395 9 0.04875446067921777 10-11 0.04689630519053615 12-13 0.05102553960982864 14-15 0.04899041693174876 16-17 0.04747144855608046 18-19 0.051718661101638454 20-21 0.05418145448743076 22-23 0.0682503460445916 24-25 0.08503273450585895 26-27 0.08703836265237244 28-29 0.07628760589642877 30-31 0.0643718151436133 32-33 0.05311965135104126 34-35 0.3002985731430464 36-37 0.47583527776032686 38-39 0.4532867083778332 40-41 0.42747899325725514 42-43 0.45822704241520096 44-45 0.4679602378321047 46-47 0.46738509446656046 48-49 0.48438869191457556 50-51 0.47335773710875134 52-53 0.47956633600347326 54-55 0.4713078671648883 56-57 0.44929019935058945 58-59 0.42724303700472416 60-61 0.3957871190891853 62-63 0.45454022596940413 64-65 0.49359098576328453 66-67 0.4947707670259396 68-69 0.5058312163633302 70-71 0.5030292358645245 72-73 0.4925881716900278 74-75 0.4854210005193987 76-77 0.46731135813764446 78-79 0.44132667582766816 80-81 0.44726982393829273 82-83 0.4588316803123117 84-85 0.4571504920130283 86-87 0.4686681065896977 88-89 0.47969906139552193 90-91 0.48347436143601796 92-93 0.4854357477851819 94-95 0.49018436736736826 96-97 0.49161485214833744 98-99 0.484890098951204 100-101 0.47164705427790155 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3390459.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.81522375658804 #Duplication Level Percentage of deduplicated Percentage of total 1 72.7345868473389 34.05085958101156 2 14.406862003875354 13.489209366834217 3 5.318081292305562 7.469014969650294 4 2.4926942585250234 4.667841578784452 5 1.4022410531388296 3.282311433168398 6 0.8205903261651736 2.3049671839148496 7 0.5369200039348422 1.7595221086518436 8 0.3769637384967465 1.4118113412676103 9 0.26937555276358705 1.1349789099463723 >10 1.4021478508047243 12.196290275927526 >50 0.13912395483593043 4.5065609083567955 >100 0.09105520917070815 7.911338061735029 >500 0.005880307460845845 1.8282373283511966 >1k 0.003224684733995939 2.53152393338344 >5k 1.2645822486258583E-4 0.5223070064375341 >10k+ 1.2645822486258583E-4 0.9332260125789822 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17966 0.5298987541214921 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13561 0.3999753425716105 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9200 0.27134969041064944 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8445 0.24908131907803635 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4970 0.14658782188488342 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3799 0.11204972542065837 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3609 0.10644576442304715 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3417 0.10078281436230316 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3404 0.10039938545194028 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.9494531566374935E-5 0.0 4 0.0 0.0 0.0 5.898906313274987E-5 0.0 5 0.0 0.0 0.0 5.898906313274987E-5 0.0 6 0.0 0.0 0.0 8.848359469912481E-5 0.0 7 0.0 0.0 0.0 8.848359469912481E-5 0.0 8 0.0 0.0 0.0 8.848359469912481E-5 0.0 9 0.0 0.0 0.0 1.1797812626549974E-4 0.0 10-11 2.9494531566374935E-5 0.0 0.0 1.9171445518143709E-4 0.0 12-13 2.9494531566374935E-5 0.0 0.0 2.3595625253099948E-4 0.0 14-15 2.9494531566374935E-5 0.0 0.0 2.654507840973744E-4 0.0 16-17 2.9494531566374935E-5 0.0 0.0 3.8342891036287417E-4 0.0 18-19 2.9494531566374935E-5 0.0 0.0 4.866597708451864E-4 0.0 20-21 2.9494531566374935E-5 0.0 0.0 7.078687575929985E-4 0.0 22-23 2.9494531566374935E-5 0.0 0.0 9.880668074735604E-4 0.0 24-25 2.9494531566374935E-5 0.0 0.0 0.0015927047045842465 0.0 26-27 2.9494531566374935E-5 0.0 0.0 0.0023153207279604324 0.0 28-29 2.9494531566374935E-5 0.0 0.0 0.0049845758347173644 0.0 30-31 2.9494531566374935E-5 0.0 0.0 0.014172122417643157 0.0 32-33 2.9494531566374935E-5 0.0 0.0 0.03378598590928249 0.0 34-35 2.9494531566374935E-5 0.0 0.0 0.06286759403372819 0.0 36-37 2.9494531566374935E-5 0.0 0.0 0.1122709344074062 0.0 38-39 2.9494531566374935E-5 0.0 0.0 0.18314629376140518 0.0 40-41 4.4241797349562405E-5 0.0 0.0 0.25930117426578525 0.0 42-43 5.898906313274987E-5 0.0 0.0 0.33815480440848866 0.0 44-45 7.373632891593735E-5 0.0 0.0 0.43772834297657043 0.0 46-47 8.848359469912481E-5 0.0 0.0 0.5597472200666636 0.0 48-49 8.848359469912481E-5 0.0 0.0 0.691396061713178 0.0 50-51 8.848359469912481E-5 0.0 0.0 0.8334859675341894 0.0 52-53 8.848359469912481E-5 0.0 0.0 0.9746762901424262 0.0 54-55 8.848359469912481E-5 0.0 0.0 1.126646864038173 0.0 56-57 8.848359469912481E-5 0.0 0.0 1.314512282850198 0.0 58-59 8.848359469912481E-5 0.0 0.0 1.526430492154602 0.0 60-61 8.848359469912481E-5 0.0 0.0 1.761634634130659 0.0 62-63 8.848359469912481E-5 0.0 0.0 1.9934174104450166 0.0 64-65 8.848359469912481E-5 0.0 0.0 2.2285330688263745 0.0 66-67 8.848359469912481E-5 0.0 0.0 2.508023249949343 0.0 68-69 8.848359469912481E-5 0.0 0.0 2.8187923817984526 0.0 70-71 1.1797812626549974E-4 0.0 0.0 3.156843955346459 0.0 72-73 1.1797812626549974E-4 0.0 0.0 3.4964587390674833 0.0 74-75 1.1797812626549974E-4 0.0 0.0 3.843992804514079 0.0 76-77 1.1797812626549974E-4 0.0 0.0 4.213456054180275 0.0 78-79 1.1797812626549974E-4 0.0 0.0 4.643722280670552 0.0 80-81 1.1797812626549974E-4 0.0 0.0 5.115973972845564 0.0 82-83 1.1797812626549974E-4 0.0 0.0 5.589449688080581 0.0 84-85 1.1797812626549974E-4 0.0 0.0 6.06580112014332 0.0 86-87 1.1797812626549974E-4 0.0 0.0 6.555793773055507 0.0 88-89 1.1797812626549974E-4 0.0 0.0 7.077802739982993 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 19105 0.0 25.926481 1 TACATGG 19330 0.0 25.075232 2 ACATGGG 19565 0.0 23.92645 3 GTATCAA 30435 0.0 23.750395 1 TACCTGG 2670 0.0 23.424149 2 GAGTACT 14890 0.0 22.10086 12-13 AGTACTT 15275 0.0 21.15634 12-13 GTACTTT 15775 0.0 20.74106 14-15 TCAACGC 35160 0.0 20.456156 4 CATGGGG 7395 0.0 20.438719 4 ATCAACG 35235 0.0 20.412615 3 TATCAAC 35295 0.0 20.404762 2 CAACGCA 35270 0.0 20.392357 5 GGTATCA 22865 0.0 19.983898 1 AACGCAG 36020 0.0 19.953905 6 CATGGGA 11315 0.0 19.764626 4 GTGTAGC 1680 0.0 19.467678 1 ACCGACG 155 9.836658E-4 18.328995 8 ACGCAGA 39090 0.0 18.314928 7 CGCAGAG 39220 0.0 18.218 8 >>END_MODULE