FastQCFastQC Report
Thu 26 May 2016
SRR938709_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938709_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3390459
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173960.5130868711286584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145140.4280836311543659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100950.29774729616255496No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT93350.27533145217211No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45060.13290235923808547No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40740.1201607216014115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35080.10346681673484327No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34640.10216905734592278No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG188400.024.1449761
TACATGG190850.023.3724212
ACATGGG187000.022.762443
CATGGGG71750.021.825294
TACCTGG27900.021.4306012
GAGTACT156350.020.97394812-13
GTATCAA311300.020.8096161
TAGCGCG1605.0307986E-520.7608934
AGTACTT161900.020.3428912-13
GGTATCA242750.019.8182981
GTACTTT169100.019.4065614-15
GTGTAGC16600.019.2252061
GTACGAC1507.7763805E-418.981393
CATGGGA109700.017.951864
ATCAACG363700.017.71843
TCAACGC364450.017.6819364
TATCAAC365950.017.5835252
GTGTAAG13550.017.5766051
CAACGCA367150.017.5519055
TACTTTT189200.017.35741214-15
Produced by FastQC (version 0.11.5)