##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938709_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3390459 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.023083895130426 33.0 31.0 34.0 30.0 34.0 2 32.239977831910075 34.0 31.0 34.0 30.0 34.0 3 32.32141046389294 34.0 31.0 34.0 30.0 34.0 4 35.94904937650035 37.0 35.0 37.0 35.0 37.0 5 35.86263806758908 37.0 35.0 37.0 35.0 37.0 6 35.900266306125516 37.0 35.0 37.0 35.0 37.0 7 35.837021771978364 37.0 35.0 37.0 35.0 37.0 8 35.804855330797395 37.0 35.0 37.0 33.0 37.0 9 37.45579551323287 39.0 37.0 39.0 34.0 39.0 10-11 37.38324987855626 39.0 37.0 39.0 34.0 39.0 12-13 37.30971956894332 39.0 37.0 39.0 34.0 39.0 14-15 38.52043381146918 40.0 38.0 41.0 34.0 41.0 16-17 38.45820197206337 40.0 38.0 41.0 34.0 41.0 18-19 38.49918285400295 40.0 38.0 41.0 33.5 41.0 20-21 38.580331453646835 40.0 38.0 41.0 34.0 41.0 22-23 38.535206884967494 40.0 38.0 41.0 34.0 41.0 24-25 38.458371860565194 40.0 38.0 41.0 34.0 41.0 26-27 38.296752740558134 40.0 38.0 41.0 33.5 41.0 28-29 38.137677671371335 40.0 38.0 41.0 33.0 41.0 30-31 37.90303938198338 40.0 37.0 41.0 33.0 41.0 32-33 37.66832071999691 40.0 36.5 41.0 33.0 41.0 34-35 37.61383266985385 40.0 37.0 41.0 33.0 41.0 36-37 37.51532845552771 40.0 36.5 41.0 32.0 41.0 38-39 37.40988078605287 40.0 36.0 41.0 32.0 41.0 40-41 37.24594737762645 40.0 36.0 41.0 31.0 41.0 42-43 37.05417835756162 40.0 36.0 41.0 31.0 41.0 44-45 36.776058639847875 40.0 36.0 41.0 30.5 41.0 46-47 36.438363065295874 39.5 35.0 41.0 30.0 41.0 48-49 36.12707512463652 39.0 35.0 41.0 29.5 41.0 50-51 35.884749085595786 39.0 35.0 41.0 28.5 41.0 52-53 35.67859455017742 39.0 35.0 41.0 28.0 41.0 54-55 35.4430765863855 39.0 35.0 40.0 27.5 41.0 56-57 35.193344028050475 38.0 34.5 40.0 27.0 41.0 58-59 34.90182037299374 38.0 34.0 40.0 26.0 41.0 60-61 34.56401124449521 38.0 34.0 40.0 26.0 41.0 62-63 34.21105711645532 37.0 34.0 40.0 26.0 41.0 64-65 33.85358265650757 37.0 33.0 39.5 25.0 41.0 66-67 33.46542960702371 36.0 33.0 39.0 23.5 40.5 68-69 33.03323266849709 36.0 32.5 39.0 22.5 40.0 70-71 32.556144463035835 35.0 32.0 38.0 22.0 40.0 72-73 32.21276750434086 35.0 32.0 37.5 21.5 40.0 74-75 31.7893942973503 35.0 31.0 37.0 20.0 39.0 76-77 29.747650391879095 33.0 28.5 35.0 17.0 37.5 78-79 30.80354061205282 34.0 30.5 36.0 18.0 38.0 80-81 30.84860501188777 34.0 31.0 36.0 18.0 37.5 82-83 30.540730768311903 34.0 31.0 35.0 15.0 37.0 84-85 30.157749437465547 34.0 30.5 35.0 9.0 36.5 86-87 29.627492619730837 34.0 30.0 35.0 4.5 36.0 88-89 29.12064782379023 34.0 29.0 35.0 2.0 36.0 90-91 28.712727981668557 34.0 29.0 35.0 2.0 35.0 92-93 28.437317779097167 34.0 29.0 35.0 2.0 35.0 94-95 28.05509696474725 34.0 29.0 35.0 2.0 35.0 96-97 27.548596369990022 33.5 27.5 35.0 2.0 35.0 98-99 26.843052813793058 33.0 26.5 35.0 2.0 35.0 100-101 23.685914945439542 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05407553332465653 1101 2 0.10967406114171752 1101 3 0.0861397293815287 1101 4 0.03042288898957679 1101 5 0.0801785689709078 1101 6 0.11347649311065311 1101 7 0.04716849680483648 1101 8 0.044253867535559266 1101 9 0.1364233357827942 1101 10-11 0.09983400121156194 1101 12-13 0.19402272897654882 1101 14-15 0.21481321904038708 1101 16-17 0.09384132113415689 1101 18-19 0.12485879415697809 1101 20-21 0.1056056898617399 1101 22-23 0.06758329386779138 1101 24-25 0.08158106375364582 1101 26-27 0.0938450862916298 1101 28-29 0.15578947197342785 1101 30-31 0.08003994915809898 1101 32-33 0.21658255745695243 1101 34-35 0.11208284884881436 1101 36-37 0.2586011075606862 1101 38-39 0.19939151827414747 1101 40-41 0.26089809587980284 1101 42-43 0.2279572298462469 1101 44-45 0.3453612950109317 1101 46-47 0.2220074361775275 1101 48-49 0.1310816280968652 1101 50-51 0.05563009348197312 1101 52-53 0.13649521890754102 1101 54-55 0.15389988384873732 1101 56-57 0.0864030358875496 1101 58-59 0.07887227643798411 1101 60-61 0.1636658869642318 1101 62-63 0.1244030394369986 1101 64-65 0.13083436165147333 1101 66-67 0.032866119741719046 1101 68-69 0.10535934419319659 1101 70-71 0.1304599432678728 1101 72-73 0.03412318126748204 1101 74-75 0.17632827720227695 1101 76-77 0.10495878357865251 1101 78-79 0.14082375742582798 1101 80-81 0.23285312331173813 1101 82-83 0.16043301408411992 1101 84-85 0.2280114782845466 1101 86-87 0.26615040938209944 1101 88-89 0.4691948121667231 1101 90-91 0.4686455645831451 1101 92-93 0.5461817410775893 1101 94-95 0.4278108237129352 1101 96-97 0.2770441090113174 1101 98-99 0.29093128000556945 1101 100-101 1.011172906877988 1102 1 0.0759522454157775 1102 2 0.0874875987144037 1102 3 0.0774169136810201 1102 4 0.06765140974844996 1102 5 0.09024475537065513 1102 6 0.13490802653430478 1102 7 0.13828350287952418 1102 8 0.07911547041380373 1102 9 0.17965463338207144 1102 10-11 0.15364479775958984 1102 12-13 0.09765343942126492 1102 14-15 0.1586455777934006 1102 16-17 0.26924171611479863 1102 18-19 0.24465998312818726 1102 20-21 0.29710051084730793 1102 22-23 0.21175668578227658 1102 24-25 0.07071294016913754 1102 26-27 0.13834896250789797 1102 28-29 0.1612554321742934 1102 30-31 0.18349474267745336 1102 32-33 0.2074140034677825 1102 34-35 0.19592907501524337 1102 36-37 0.19229592821049124 1102 38-39 0.2339409924260849 1102 40-41 0.20041871316437465 1102 42-43 0.1952101556858068 1102 44-45 -0.01104969751644802 1102 46-47 -0.04042790658765938 1102 48-49 -0.001656604048555721 1102 50-51 -0.027873504602560217 1102 52-53 0.018312174646538892 1102 54-55 -0.0446027936589104 1102 56-57 -0.09561278124615313 1102 58-59 -0.13365604739018266 1102 60-61 -0.17576326023461064 1102 62-63 -0.23124379033686182 1102 64-65 -0.0900514851365557 1102 66-67 -0.1899242579074567 1102 68-69 -0.2016894206388642 1102 70-71 -0.1529588162421902 1102 72-73 -0.07080131743783369 1102 74-75 -0.009679597787297212 1102 76-77 -0.20442542044133205 1102 78-79 -0.1662406767999336 1102 80-81 -0.1062736885318607 1102 82-83 -0.16522581396834823 1102 84-85 -0.14744020597940732 1102 86-87 -0.09867043068767245 1102 88-89 0.023195180397475923 1102 90-91 0.0816286086202993 1102 92-93 0.02648965026001804 1102 94-95 0.023965218726036852 1102 96-97 0.008444958404382419 1102 98-99 0.20239976521730085 1102 100-101 0.6873332674531234 1103 1 0.026014345963837116 1103 2 0.07184060782658008 1103 3 -0.005742074174484912 1103 4 -0.016533846281305387 1103 5 0.04419783621982987 1103 6 0.04951816840742396 1103 7 0.0610535449076508 1103 8 0.10482471221865097 1103 9 0.1510790290592965 1103 10-11 -0.02123416897824626 1103 12-13 -3.4238068831626833E-4 1103 14-15 0.003948372290551561 1103 16-17 0.015982927278123782 1103 18-19 -0.05689768346091739 1103 20-21 0.007846830521089032 1103 22-23 -0.032412115848607925 1103 24-25 -0.008772231342248915 1103 26-27 8.408091176193011E-4 1103 28-29 0.046570857664356424 1103 30-31 0.010293948582628332 1103 32-33 0.041872445062175245 1103 34-35 -0.044285068726111376 1103 36-37 -0.15000737222321447 1103 38-39 -0.01995163826609314 1103 40-41 -0.010379547983603743 1103 42-43 0.1615160468667156 1103 44-45 0.22388718327745494 1103 46-47 0.15517850485152707 1103 48-49 0.06046347363718496 1103 50-51 0.08398349157935314 1103 52-53 0.00625739743374254 1103 54-55 -0.07294903946852571 1103 56-57 0.08808940532028231 1103 58-59 -0.16552739097171099 1103 60-61 -0.10402424500828289 1103 62-63 -0.001997137009027483 1103 64-65 -0.010782698230833887 1103 66-67 -0.030849069208159108 1103 68-69 -0.019581924434298514 1103 70-71 -0.041088937841173845 1103 72-73 0.11094969429773727 1103 74-75 0.1497016467652621 1103 76-77 0.07027126060390998 1103 78-79 0.021111120375074677 1103 80-81 0.15409645200627153 1103 82-83 0.12622747088749975 1103 84-85 0.05001968630873321 1103 86-87 0.21963059073935298 1103 88-89 0.24886231889260912 1103 90-91 0.309461060186365 1103 92-93 0.21060943160888002 1103 94-95 0.14997010058279514 1103 96-97 0.1623037194044663 1103 98-99 0.30992563097118975 1103 100-101 0.7412037737362525 1104 1 0.0464811187510108 1104 2 0.06308966267944527 1104 3 0.05411766798974327 1104 4 0.10675494235709238 1104 5 0.02859350705537622 1104 6 0.09971806180245579 1104 7 0.03162872121097138 1104 8 0.03962854491062018 1104 9 -0.002692094365926323 1104 10-11 0.10699236621138652 1104 12-13 -0.014959596554646737 1104 14-15 -0.011385811024204884 1104 16-17 0.14208998933028028 1104 18-19 0.11359995535172374 1104 20-21 0.08101929475628822 1104 22-23 0.07621585571032341 1104 24-25 0.08653811300922598 1104 26-27 0.08664218306451232 1104 28-29 0.06807679269783051 1104 30-31 0.058773592447920464 1104 32-33 0.06184261819896619 1104 34-35 0.10183794773661248 1104 36-37 0.03868178273803835 1104 38-39 0.09745552839264349 1104 40-41 0.13720083902803282 1104 42-43 0.19355939591950033 1104 44-45 0.08729793540928199 1104 46-47 -0.015731892112079038 1104 48-49 0.13504543792270596 1104 50-51 0.10052906193227074 1104 52-53 -0.01049673205531576 1104 54-55 0.0508016495214747 1104 56-57 0.06061900600220582 1104 58-59 0.028585845738909654 1104 60-61 0.09332819849453244 1104 62-63 -0.03497682211379072 1104 64-65 0.09347833932543637 1104 66-67 -0.08577142816459826 1104 68-69 0.07998798593250456 1104 70-71 -0.052846186633694 1104 72-73 -0.21054248818642662 1104 74-75 -0.19176806656038892 1104 76-77 -0.09884703566415354 1104 78-79 0.034057864763326506 1104 80-81 -0.08423059487993001 1104 82-83 -0.018177566942000567 1104 84-85 0.12853775010966473 1104 86-87 0.22393630137191067 1104 88-89 0.36970398944402305 1104 90-91 0.35595812055624165 1104 92-93 0.4756890928419857 1104 94-95 0.4777409485936879 1104 96-97 0.5067081577172878 1104 98-99 0.47847574893063793 1104 100-101 0.8243180412522229 1105 1 -0.06431713341592271 1105 2 -0.06142751087778464 1105 3 0.004413911337309173 1105 4 -0.04842548082415732 1105 5 -0.06605112713327088 1105 6 -0.08832355995376417 1105 7 -0.048634008696041064 1105 8 -0.06526292084278396 1105 9 -0.15155426463554278 1105 10-11 -0.01716489723297343 1105 12-13 -0.12931000310717877 1105 14-15 -0.15547310815744453 1105 16-17 -0.16137451920042878 1105 18-19 -0.08981213752065997 1105 20-21 -0.07560873505403976 1105 22-23 -0.11985227175639324 1105 24-25 -0.2002265573333375 1105 26-27 -0.10352144043281442 1105 28-29 -0.08059884370577919 1105 30-31 -0.19202508398510076 1105 32-33 -0.1636170794765448 1105 34-35 -0.136216276691556 1105 36-37 -0.16470081737578113 1105 38-39 -0.1289457893742494 1105 40-41 -0.1395749606911636 1105 42-43 -0.2648371521656898 1105 44-45 -0.17251071163480702 1105 46-47 -0.030381289895906605 1105 48-49 -0.05799317011217653 1105 50-51 -0.15242929894702684 1105 52-53 -0.1820357904030061 1105 54-55 -0.0583996973174834 1105 56-57 -0.17053422619826364 1105 58-59 -0.1293348082681618 1105 60-61 -0.11051317289099671 1105 62-63 -0.13968233580067135 1105 64-65 -0.16742708925760752 1105 66-67 -0.21626896339345336 1105 68-69 -0.09837987675279436 1105 70-71 -0.2948353447555121 1105 72-73 -0.28426248008777577 1105 74-75 -0.30520025926240635 1105 76-77 -0.2749491921352778 1105 78-79 -0.33186599612250944 1105 80-81 -0.2995371307079502 1105 82-83 -0.16587631725460739 1105 84-85 -0.10614002337771566 1105 86-87 0.0063911353827244 1105 88-89 0.11724508104665077 1105 90-91 0.10579087558088318 1105 92-93 0.1184774554917496 1105 94-95 0.1596650830147901 1105 96-97 0.03381106300910375 1105 98-99 0.1143903296671347 1105 100-101 0.4953727291508123 1106 1 -0.071256230640099 1106 2 -0.11819632227602739 1106 3 -0.047001301041561305 1106 4 -0.07191569122919361 1106 5 -0.09003299186008462 1106 6 -0.1307998704855251 1106 7 -0.014314556501197728 1106 8 -0.15420605139881616 1106 9 -0.05024697800803324 1106 10-11 -0.08270359594146726 1106 12-13 -0.011777856221101501 1106 14-15 -0.012198294267470544 1106 16-17 -0.10805518342889542 1106 18-19 -0.18800864669468353 1106 20-21 -0.12640998172025064 1106 22-23 -0.10459426029337493 1106 24-25 -0.08135835254071111 1106 26-27 -0.05860746655713456 1106 28-29 -0.05745442392880307 1106 30-31 -0.14754294693478442 1106 32-33 -0.021295866211779924 1106 34-35 0.009638584918590709 1106 36-37 0.06523790649087147 1106 38-39 0.0025046979309237827 1106 40-41 0.007697053686449351 1106 42-43 -0.060145961663693015 1106 44-45 -0.014633068524460668 1106 46-47 -3.6200644795769676E-4 1106 48-49 -0.025142658987064692 1106 50-51 -0.13901719921908295 1106 52-53 -0.01732168993370209 1106 54-55 0.09236903111242611 1106 56-57 0.03949811807217429 1106 58-59 0.016731221148127418 1106 60-61 -0.05350723262552748 1106 62-63 -0.0597468905216374 1106 64-65 0.11852476501787379 1106 66-67 0.1243706689765176 1106 68-69 -3.130390642880343E-4 1106 70-71 -0.05521956845957021 1106 72-73 0.021887079711660817 1106 74-75 0.1823597087814015 1106 76-77 0.16985424650093606 1106 78-79 -0.01428152565455676 1106 80-81 -0.0806152641344191 1106 82-83 0.15154328709346743 1106 84-85 0.1507577904065407 1106 86-87 0.3151535287784455 1106 88-89 0.37598398904948027 1106 90-91 0.36063736407973224 1106 92-93 0.2846457768415078 1106 94-95 0.4290889749525455 1106 96-97 0.44185350532938017 1106 98-99 0.5371928395932279 1106 100-101 1.0713460347566617 1107 1 -0.12805603435387525 1107 2 -0.04882602170404482 1107 3 -0.10023149710156787 1107 4 -0.058861352311794235 1107 5 -0.024062201616622758 1107 6 -0.021575549091195967 1107 7 -0.11964090844865893 1107 8 -0.05959957221333667 1107 9 -0.1284263188830721 1107 10-11 -0.04181611423454967 1107 12-13 -0.051667767144046195 1107 14-15 -0.1407569391268808 1107 16-17 -0.04648900034326431 1107 18-19 -0.05296102814423875 1107 20-21 -0.11358581798135248 1107 22-23 -0.13264496234724987 1107 24-25 -0.16671871525259263 1107 26-27 -0.11677161613140896 1107 28-29 -0.10909792951722608 1107 30-31 -0.09563798740141749 1107 32-33 -0.07642649747732122 1107 34-35 -0.07520461231948161 1107 36-37 -0.13556459615846705 1107 38-39 -0.11820945851685138 1107 40-41 -0.05120260368273222 1107 42-43 -5.67785629051798E-4 1107 44-45 -0.1479211150763149 1107 46-47 -0.18148881639660885 1107 48-49 -0.32107628883787953 1107 50-51 -0.1905014397671536 1107 52-53 -0.17477621608866656 1107 54-55 -0.20082881600357894 1107 56-57 -0.22476134516853108 1107 58-59 -0.21811167156787548 1107 60-61 -0.29853286468281937 1107 62-63 -0.17808190307664518 1107 64-65 -0.1469944625053543 1107 66-67 -0.21943948500724275 1107 68-69 -0.1299259393150436 1107 70-71 -0.2548060274943893 1107 72-73 -0.1816467248478162 1107 74-75 -0.20360382513626618 1107 76-77 -0.22856471843726212 1107 78-79 -0.4028644713748015 1107 80-81 -0.20063141250453143 1107 82-83 -0.13972328108206256 1107 84-85 -0.33375308625158695 1107 86-87 -0.4084743817999552 1107 88-89 -0.059410915165276634 1107 90-91 0.07063721616901475 1107 92-93 0.22406011130418335 1107 94-95 0.21079294122193915 1107 96-97 0.24286386604011767 1107 98-99 0.2115079259643835 1107 100-101 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8693.0 13 11946.0 14 14735.0 15 19556.0 16 25181.0 17 33955.0 18 31664.0 19 21134.0 20 18663.0 21 19503.0 22 20485.0 23 22644.0 24 26628.0 25 31521.0 26 37886.0 27 46818.0 28 56715.0 29 69556.0 30 85018.0 31 103125.0 32 125865.0 33 159197.0 34 211695.0 35 296652.0 36 440824.0 37 705196.0 38 654653.0 39 75985.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.218237172786964 19.706392864693413 14.789854656206824 22.2855153063128 2 15.694659631630998 21.492458690696452 39.428230808866886 23.38465086880567 3 19.703703834790513 25.648385661056512 29.960751626844623 24.687158877308352 4 12.936065588759515 17.30860039894303 36.99369318431516 32.76164082798228 5 13.202784637714243 38.2935171904453 33.85621238894203 14.647485782898423 6 30.985126202676394 36.76652630219095 17.436901611256765 14.811445883875901 7 27.962467618691157 33.51788061734414 20.74459534829945 17.77505641566525 8 26.206422198292323 34.7320819983371 19.771777213645706 19.28971858972487 9 26.66585851650175 17.078749514446272 20.639919255770383 35.61547271328159 10-11 24.908515336713997 27.044420829156167 26.910987568349892 21.136076265779945 12-13 26.356180682320595 24.49237699084401 27.59263568738038 21.55880663945501 14-15 22.82081479967426 25.69919122901866 26.777852399074696 24.702141572232385 16-17 21.6605461602591 29.14718813843351 27.213230027266867 21.979035674040524 18-19 21.41250214020168 27.403238182289293 30.069579228607164 21.114680448901865 20-21 22.490822406468034 26.254089233063905 29.99166631887693 21.263422041591127 22-23 22.011547698998868 26.406247649654517 29.870660580175134 21.711544071171485 24-25 22.05089340410841 26.553971011004705 30.025757574416918 21.36937801046997 26-27 21.77991370778537 26.67325181727003 30.118230391689142 21.428604083255458 28-29 21.326669928761856 27.1040440247176 30.230080941842978 21.339205104677568 30-31 21.562095279724662 26.704584836448397 30.0407702909842 21.692549592842738 32-33 21.32987008543681 26.899543690102135 29.985379560702548 21.785206663758505 34-35 22.123858565762024 26.678312989140014 29.742279026516893 21.45554941858107 36-37 22.057557435233395 26.464405045188133 29.828901199701995 21.64913631987648 38-39 21.716651261491382 26.845962553551235 30.030947514884907 21.406438670072472 40-41 21.746101976886184 26.775879156858927 29.963276286214892 21.514742580039997 42-43 21.592660558035558 26.940060132620935 29.42534221130362 22.041937098039888 44-45 21.73608025388226 26.99765705454873 29.2487399312852 22.017522760283807 46-47 22.05119409581819 27.2321448980883 28.783697431328743 21.932963574764766 48-49 22.15248731808879 27.338894232314857 28.561929225511946 21.94668922408441 50-51 21.773425022379065 27.486834324107896 28.69413896995822 22.045601683554814 52-53 22.199752304923905 27.651639497778913 28.285329508482484 21.8632786888147 54-55 21.65122775411825 27.341312783903298 28.566751581423045 22.440707880555404 56-57 21.969945662224497 27.684717614930605 28.184354979664995 22.160981743179907 58-59 22.271252203805254 27.200741496132142 28.250869908573883 22.27713639148872 60-61 21.984214730863062 27.304159239532222 28.799496348482258 21.91212968112246 62-63 21.89446915594614 27.31071220740319 28.758466036604485 22.03635260004619 64-65 21.61126266384581 27.3803487964314 28.567090768536058 22.441297771186733 66-67 21.646451125000905 27.57410992946448 28.169228026207378 22.61021091932724 68-69 21.598843687005623 27.451240350970075 28.389586989148725 22.560328972875578 70-71 21.712151361960032 27.444899767416103 28.204325786167043 22.638623084456817 72-73 21.762805567033844 27.22244982169081 28.31920397798646 22.695540633288886 74-75 21.450930390250996 27.76775946855573 28.05344350130764 22.727866639885633 76-77 21.575752207817132 27.316025080359747 28.273507301573243 22.83471541024988 78-79 21.574903072027727 27.383411666939832 28.225930063744315 22.815755197288127 80-81 21.572052865416286 27.6753424352488 28.07005306214484 22.682551637190073 82-83 21.670399199636392 27.437479704075468 28.04323839338567 22.84888270290247 84-85 21.41277921366989 27.340059266311727 27.969015404698894 23.27814611531949 86-87 21.472461774845296 27.293298813533262 28.100419947576338 23.1338194640451 88-89 21.45137509478629 27.203477038598827 27.882165381272078 23.462982485342803 90-91 21.38955482364355 27.19740475243249 28.03469302338651 23.378347400537447 92-93 21.565147963741783 27.16639546444891 28.0842652867945 23.1841912850148 94-95 21.29155136143131 26.953766563951913 28.032780236885436 23.721901837731338 96-97 21.190172361643715 26.761926152465808 28.155159545530495 23.892741940359986 98-99 21.083993630940522 26.838710514684834 27.867289729825227 24.210006124549416 100-101 21.131065136969987 26.36768317906344 28.47303404506652 24.028217638900053 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3167.0 1 2090.5 2 1319.0 3 1595.0 4 1300.5 5 1221.0 6 2233.5 7 4498.5 8 6787.5 9 7447.5 10 6084.0 11 4753.0 12 4650.0 13 4817.5 14 5099.5 15 5387.0 16 5653.0 17 5965.0 18 6317.5 19 6751.0 20 7477.5 21 8562.5 22 9887.5 23 11628.0 24 13824.0 25 16735.0 26 20761.0 27 25660.5 28 29870.5 29 34556.5 30 40410.5 31 46829.5 32 55494.0 33 62951.5 34 69144.5 35 75659.0 36 83386.5 37 90770.5 38 94049.5 39 94943.5 40 95784.5 41 99773.0 42 103603.5 43 107689.5 44 112748.5 45 116571.0 46 117241.5 47 123827.0 48 149776.5 49 150824.5 50 129900.5 51 121224.5 52 113559.5 53 111889.5 54 110177.5 55 101223.5 56 90702.5 57 80435.0 58 71603.0 59 62631.5 60 52460.5 61 42719.0 62 35016.5 63 27602.5 64 20563.5 65 16162.5 66 12125.5 67 9459.5 68 6826.0 69 4251.0 70 3065.0 71 2357.0 72 1945.0 73 1796.0 74 1474.5 75 1232.0 76 891.5 77 483.5 78 278.0 79 164.5 80 89.0 81 50.5 82 43.5 83 29.5 84 12.5 85 7.0 86 4.5 87 0.5 88 0.5 89 1.5 90 2.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39006517996530854 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.7696718939824962E-4 16-17 0.0023743097910931825 18-19 2.21208986747812E-4 20-21 1.4747265783187467E-5 22-23 0.0 24-25 0.0 26-27 7.373632891593735E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007712820004607046 36-37 0.016974102916448776 38-39 0.010750756755943666 40-41 0.008228974307018607 42-43 0.0035245965221818044 44-45 0.002020375412296683 46-47 0.0014304847809691844 48-49 0.0 50-51 4.1292344192924915E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.866597708451865E-4 60-61 5.751433655443113E-4 62-63 0.0 64-65 0.0 66-67 0.00116503399687181 68-69 8.110996180753107E-4 70-71 0.002049869943863058 72-73 0.0 74-75 0.0 76-77 0.09870344988687373 78-79 0.03585060311892874 80-81 1.4747265783187467E-5 82-83 0.0 84-85 0.0 86-87 0.152059057490446 88-89 0.3287607961045982 90-91 0.28503515305744737 92-93 0.0 94-95 4.4241797349562405E-5 96-97 1.4747265783187467E-5 98-99 1.6221992361506213E-4 100-101 7.816050865089359E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3390459.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.387051576884424 #Duplication Level Percentage of deduplicated Percentage of total 1 70.45322395940804 31.272108856440333 2 15.376683509972786 13.650512880771803 3 5.753804834699205 7.661832958833616 4 2.78643591803367 4.9472669923777515 5 1.5538855108464356 3.4486198157257064 6 0.9506892273573665 2.5318975060979896 7 0.5779242064336042 1.7956646090948873 8 0.4169585771874867 1.480604949683625 9 0.3058649788746352 1.221880013358399 >10 1.5622593100366666 12.87561433669557 >50 0.1483930056182539 4.5802545676929 >100 0.10349687494577002 8.471596104566181 >500 0.0067204406755522654 1.9912890564182186 >1k 0.003393489844324219 2.5545454642034766 >5k 6.653901655537686E-5 0.27570649541973863 >10k+ 1.9961704966613055E-4 1.2406053926198308 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17396 0.5130868711286584 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14514 0.4280836311543659 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10095 0.29774729616255496 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9335 0.27533145217211 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4506 0.13290235923808547 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4074 0.1201607216014115 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3508 0.10346681673484327 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3464 0.10216905734592278 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.9494531566374935E-5 0.0 2 0.0 0.0 0.0 2.9494531566374935E-5 0.0 3 0.0 0.0 0.0 8.848359469912481E-5 0.0 4 0.0 0.0 0.0 8.848359469912481E-5 0.0 5 0.0 0.0 0.0 1.1797812626549974E-4 0.0 6 0.0 0.0 0.0 1.7696718939824962E-4 0.0 7 0.0 0.0 0.0 1.7696718939824962E-4 0.0 8 0.0 0.0 0.0 1.7696718939824962E-4 0.0 9 0.0 0.0 0.0 2.3595625253099948E-4 0.0 10-11 0.0 0.0 0.0 3.5393437879649924E-4 0.0 12-13 0.0 0.0 0.0 4.42417973495624E-4 0.0 14-15 0.0 0.0 0.0 4.7191250506199895E-4 0.0 16-17 0.0 0.0 0.0 6.193851628938737E-4 0.0 18-19 0.0 0.0 0.0 7.078687575929985E-4 0.0 20-21 0.0 0.0 0.0 9.143304785576231E-4 0.0 22-23 0.0 0.0 0.0 0.0012092757942213724 0.0 24-25 0.0 0.0 0.0 0.0018286609571152462 0.0 26-27 0.0 0.0 0.0 0.002684002372540119 0.0 28-29 0.0 0.0 0.0 0.0054122465424298015 0.0 30-31 0.0 0.0 0.0 0.014806254846320217 0.0 32-33 0.0 0.0 0.0 0.03467082185627374 0.0 34-35 0.0 0.0 0.0 0.06392939717011768 0.0 36-37 0.0 0.0 0.0 0.11371616645415858 0.0 38-39 0.0 0.0 0.0 0.1852109109710514 0.0 40-41 0.0 0.0 0.0 0.2622506274224228 0.0 42-43 0.0 0.0 0.0 0.3418121263227191 0.0 44-45 0.0 0.0 0.0 0.4419165664589957 0.0 46-47 0.0 0.0 0.0 0.564658059572465 0.0 48-49 0.0 0.0 0.0 0.6970442645081388 0.0 50-51 0.0 0.0 0.0 0.8401074898708405 0.0 52-53 0.0 0.0 1.4747265783187467E-5 0.9819614394393208 0.0 54-55 0.0 0.0 2.9494531566374935E-5 1.1349938164714573 0.0 56-57 0.0 0.0 2.9494531566374935E-5 1.3243192145960179 0.0 58-59 0.0 0.0 2.9494531566374935E-5 1.539245866120192 0.0 60-61 0.0 0.0 2.9494531566374935E-5 1.7767800760899926 0.0 62-63 0.0 0.0 2.9494531566374935E-5 2.009890106324837 0.0 64-65 0.0 0.0 2.9494531566374935E-5 2.2470408873842747 0.0 66-67 0.0 0.0 2.9494531566374935E-5 2.5280500368829117 0.0 68-69 0.0 0.0 2.9494531566374935E-5 2.8404118734366053 0.0 70-71 0.0 0.0 2.9494531566374935E-5 3.182474703277639 0.0 72-73 0.0 0.0 2.9494531566374935E-5 3.52475874210542 0.0 74-75 0.0 0.0 2.9494531566374935E-5 3.8760238657951622 0.0 76-77 0.0 0.0 2.9494531566374935E-5 4.248746261199442 0.0 78-79 0.0 0.0 2.9494531566374935E-5 4.680855895912618 0.0 80-81 0.0 0.0 2.9494531566374935E-5 5.155349172486675 0.0 82-83 0.0 0.0 2.9494531566374935E-5 5.631435153765316 0.0 84-85 0.0 0.0 2.9494531566374935E-5 6.109438279595771 0.0 86-87 0.0 0.0 2.9494531566374935E-5 6.6012006044019405 0.0 88-89 0.0 0.0 2.9494531566374935E-5 7.125539639323172 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18840 0.0 24.144976 1 TACATGG 19085 0.0 23.372421 2 ACATGGG 18700 0.0 22.76244 3 CATGGGG 7175 0.0 21.82529 4 TACCTGG 2790 0.0 21.430601 2 GAGTACT 15635 0.0 20.973948 12-13 GTATCAA 31130 0.0 20.809616 1 TAGCGCG 160 5.0307986E-5 20.760893 4 AGTACTT 16190 0.0 20.34289 12-13 GGTATCA 24275 0.0 19.818298 1 GTACTTT 16910 0.0 19.40656 14-15 GTGTAGC 1660 0.0 19.225206 1 GTACGAC 150 7.7763805E-4 18.98139 3 CATGGGA 10970 0.0 17.95186 4 ATCAACG 36370 0.0 17.7184 3 TCAACGC 36445 0.0 17.681936 4 TATCAAC 36595 0.0 17.583525 2 GTGTAAG 1355 0.0 17.576605 1 CAACGCA 36715 0.0 17.551905 5 TACTTTT 18920 0.0 17.357412 14-15 >>END_MODULE