FastQCFastQC Report
Thu 26 May 2016
SRR938708_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938708_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3401936
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181350.5330788115943392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135940.399595994751224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91630.26934663085960464No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT83160.24444904313308657No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49090.14430018671721043No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37920.11146594174611163No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34910.10261803866974571No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG25750.026.527572
GTACATG191050.026.3431991
TACATGG193100.025.572942
ACATGGG193150.024.2404693
GTATCAA294700.023.9155641
GAGTACT149000.022.66389712-13
GTATAGG11750.022.2456151
GGTATCA221000.021.2033371
GTACTTT161650.020.99355114-15
CATGGGG73850.020.87624
ATCAACG341100.020.5686153
TCAACGC342250.020.4856414
TATCAAC342600.020.4228742
AGTACTT155200.020.36706512-13
CAACGCA344250.020.3528465
GTACCTG34500.020.2496641
AACGCAG350850.020.0087726
CATGGGA112050.019.6437664
CGTATAG2752.8252543E-819.009891
ACGCAGA377950.018.4496777
Produced by FastQC (version 0.11.5)