##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938708_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3401936 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50466910606196 33.0 31.0 34.0 30.0 34.0 2 31.560248046994417 33.0 31.0 34.0 28.0 34.0 3 31.569546575832113 33.0 31.0 34.0 28.0 34.0 4 35.119660981276546 37.0 35.0 37.0 32.0 37.0 5 35.154673985636414 37.0 35.0 37.0 32.0 37.0 6 35.216391490022154 37.0 35.0 37.0 32.0 37.0 7 35.12976346409809 37.0 35.0 37.0 32.0 37.0 8 35.09597476260576 37.0 35.0 37.0 32.0 37.0 9 36.65562756030684 39.0 37.0 39.0 32.0 39.0 10-11 36.53238611778705 39.0 37.0 39.0 32.0 39.0 12-13 36.414480901463165 39.0 37.0 39.0 32.0 39.0 14-15 37.54364705861603 40.0 37.0 41.0 32.0 41.0 16-17 37.43562518518867 40.0 37.0 41.0 32.0 41.0 18-19 37.39941139398272 40.0 37.0 41.0 32.0 41.0 20-21 37.3043691886032 40.0 37.0 41.0 31.0 41.0 22-23 37.317679844653156 40.0 37.0 41.0 31.5 41.0 24-25 37.24585544819185 40.0 37.0 41.0 31.0 41.0 26-27 37.10912991896379 40.0 37.0 41.0 31.0 41.0 28-29 36.93351153578433 40.0 36.0 41.0 30.0 41.0 30-31 36.69078489424845 39.0 36.0 41.0 30.0 41.0 32-33 36.49042750951223 39.0 35.5 41.0 30.0 41.0 34-35 36.18432871753025 39.0 35.0 41.0 29.5 41.0 36-37 35.96055951669872 39.0 35.0 41.0 28.5 41.0 38-39 35.788966488493614 39.0 35.0 41.0 27.0 41.0 40-41 35.66844114645308 39.0 35.0 40.5 27.0 41.0 42-43 35.63319004237587 39.0 35.0 41.0 27.0 41.0 44-45 35.426620459644155 39.0 35.0 41.0 26.0 41.0 46-47 35.07877852493404 39.0 35.0 41.0 24.0 41.0 48-49 34.76655351541005 39.0 34.0 41.0 23.5 41.0 50-51 33.62401996980543 38.0 32.5 40.0 21.5 40.5 52-53 33.69646754083557 38.0 33.0 39.5 22.0 40.5 54-55 34.1836783525616 38.0 34.0 40.0 22.0 41.0 56-57 34.08280681941106 38.0 34.0 40.0 20.5 41.0 58-59 33.87985164917858 38.0 34.0 40.0 20.0 41.0 60-61 33.57352357598732 38.0 33.0 40.0 18.5 41.0 62-63 33.190764317729666 37.0 33.0 40.0 16.5 41.0 64-65 32.80724358130194 36.5 32.0 40.0 13.0 41.0 66-67 32.41901214484929 36.0 32.0 39.0 10.0 41.0 68-69 32.00547791022524 35.5 31.0 39.0 8.0 40.5 70-71 31.57875706656445 35.0 31.0 38.5 7.0 40.0 72-73 31.15287001283975 35.0 31.0 37.5 3.5 40.0 74-75 30.689620263285377 35.0 30.0 37.0 2.0 39.0 76-77 30.23499119324996 35.0 30.0 36.5 2.0 39.0 78-79 29.798997541399956 34.0 29.5 36.0 2.0 38.5 80-81 29.325057555462536 34.0 29.0 35.5 2.0 37.5 82-83 28.907733713979333 34.0 29.0 35.0 2.0 37.0 84-85 28.55858722797842 34.0 29.0 35.0 2.0 36.5 86-87 28.143423920967354 34.0 27.5 35.0 2.0 36.0 88-89 27.687573340591946 34.0 26.5 35.0 2.0 36.0 90-91 27.233669151918203 34.0 26.0 35.0 2.0 35.0 92-93 26.77966560805377 33.0 25.0 35.0 2.0 35.0 94-95 26.264337277362067 33.0 24.5 35.0 2.0 35.0 96-97 25.661919859750448 33.0 21.5 35.0 2.0 35.0 98-99 24.905352422855692 32.5 10.0 35.0 2.0 35.0 100-101 22.448855151889983 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10841634576226156 1101 2 0.13790735580840163 1101 3 0.23471550925730256 1101 4 0.0730712003058116 1101 5 0.14580968779117143 1101 6 0.1897507794676443 1101 7 0.21760163444535152 1101 8 0.2558668478228938 1101 9 0.29201286077650224 1101 10-11 0.20505669954446404 1101 12-13 0.20543527614317014 1101 14-15 0.22770154536051734 1101 16-17 0.40200895030722705 1101 18-19 0.4534729419855239 1101 20-21 0.4348535733364969 1101 22-23 0.3946989652701376 1101 24-25 0.5519347656378599 1101 26-27 0.4674995567405418 1101 28-29 0.5465798778115385 1101 30-31 0.5603776286508051 1101 32-33 0.4361179778780624 1101 34-35 0.4897361238408351 1101 36-37 0.5705245299312054 1101 38-39 0.6251433457780209 1101 40-41 0.6362294151708028 1101 42-43 0.712810134668814 1101 44-45 0.6175316281636825 1101 46-47 0.5090302726318967 1101 48-49 0.4296364500222012 1101 50-51 0.492829491515657 1101 52-53 0.585105366030028 1101 54-55 0.6292484222198453 1101 56-57 0.6101329011072494 1101 58-59 0.5947257762318472 1101 60-61 0.5321679011814524 1101 62-63 0.3792863646113105 1101 64-65 0.3862458689853199 1101 66-67 0.3269756964207673 1101 68-69 0.3617615267443881 1101 70-71 0.2874834830955244 1101 72-73 0.13278688674929384 1101 74-75 0.09721278273039502 1101 76-77 0.06859081742482331 1101 78-79 -0.11929158474162449 1101 80-81 -0.06237522792365979 1101 82-83 -0.020097644896779343 1101 84-85 -0.1772999815316112 1101 86-87 -0.23202711343953553 1101 88-89 -0.10276103501076506 1101 90-91 -0.20954997604547998 1101 92-93 -0.237219777338062 1101 94-95 -0.28730825347417976 1101 96-97 -0.3289836337109904 1101 98-99 -0.23610035588698608 1101 100-101 0.25550061497230914 1102 1 0.04153016396189102 1102 2 -0.011519693804931563 1102 3 0.06513225701746705 1102 4 0.15766290612905465 1102 5 0.12591457312827714 1102 6 0.19720449659499195 1102 7 0.2381823036090367 1102 8 0.18579941775196573 1102 9 0.1974439405711763 1102 10-11 0.3113623975377138 1102 12-13 0.2745168740756512 1102 14-15 0.3918995273570971 1102 16-17 0.3689727170704131 1102 18-19 0.3194079798048861 1102 20-21 0.34597099539736575 1102 22-23 0.3794490185601447 1102 24-25 0.28832409916184787 1102 26-27 0.38048855053241937 1102 28-29 0.41990058041884026 1102 30-31 0.39012126727963903 1102 32-33 0.338774534668012 1102 34-35 0.4130200861287108 1102 36-37 0.5315950170615054 1102 38-39 0.4862572593038763 1102 40-41 0.5318542058171829 1102 42-43 0.6007132355517797 1102 44-45 0.6365018135599669 1102 46-47 0.6707624827044398 1102 48-49 0.6062010249400203 1102 50-51 0.4373145378746628 1102 52-53 0.44443403102584256 1102 54-55 0.5093684008773565 1102 56-57 0.5147241832583376 1102 58-59 0.4240960537768288 1102 60-61 0.3373435861070675 1102 62-63 0.26889898262717793 1102 64-65 0.3006544292811526 1102 66-67 0.16818456546767635 1102 68-69 0.2913950106137406 1102 70-71 0.2626022767949898 1102 72-73 0.2894943451003371 1102 74-75 0.3107304399293156 1102 76-77 0.2306756021662686 1102 78-79 0.22156298846826417 1102 80-81 0.19540663652508172 1102 82-83 0.14181820057410022 1102 84-85 0.11623041231137066 1102 86-87 0.006052407175424435 1102 88-89 0.025243429434535614 1102 90-91 -0.17534348088659968 1102 92-93 -0.2995058063516609 1102 94-95 -0.2500157038813704 1102 96-97 -0.3524322985479209 1102 98-99 -0.4973225706066806 1102 100-101 -0.012756940624729651 1103 1 0.061737054274566106 1103 2 0.20737878001157384 1103 3 0.2596102914791416 1103 4 0.3269340412055044 1103 5 0.28454387231565903 1103 6 0.2156694582971852 1103 7 0.2228058953880705 1103 8 0.17970059389075743 1103 9 0.2843762876411233 1103 10-11 0.3085806256800723 1103 12-13 0.38576273407902306 1103 14-15 0.4746459318427796 1103 16-17 0.4472564663539984 1103 18-19 0.49647489345214524 1103 20-21 0.4971035211705299 1103 22-23 0.5289094743181479 1103 24-25 0.5189404126861845 1103 26-27 0.4596825352796614 1103 28-29 0.5863493764014152 1103 30-31 0.5656496069287584 1103 32-33 0.48614402805451107 1103 34-35 0.6128580504777688 1103 36-37 0.7004525737007512 1103 38-39 0.6421011576156914 1103 40-41 0.7270891497345957 1103 42-43 0.8507311862297939 1103 44-45 0.8522623533297278 1103 46-47 0.8172556978665781 1103 48-49 0.737186539196486 1103 50-51 0.9100981994137811 1103 52-53 0.8034068945257715 1103 54-55 0.7602361897919039 1103 56-57 0.7229389578716976 1103 58-59 0.7581492287398177 1103 60-61 0.740080461828498 1103 62-63 0.5545827919969426 1103 64-65 0.6621863984666376 1103 66-67 0.6244836722367566 1103 68-69 0.5925622842336509 1103 70-71 0.6247980970476554 1103 72-73 0.6460072528691043 1103 74-75 0.5352207119579084 1103 76-77 0.47580993156134 1103 78-79 0.44183945223912957 1103 80-81 0.5224228864897675 1103 82-83 0.529900583428752 1103 84-85 0.5186843542413833 1103 86-87 0.3661376942812886 1103 88-89 0.3749183601526944 1103 90-91 0.37299063138951283 1103 92-93 0.3609635345383282 1103 94-95 0.3920562009616333 1103 96-97 0.38180914468140514 1103 98-99 0.5915840918295849 1103 100-101 0.898250726194096 1104 1 0.004157434089716361 1104 2 0.13934688837262144 1104 3 0.09072795955167479 1104 4 0.22153310727415487 1104 5 0.2868448737471567 1104 6 0.22850140446928435 1104 7 0.2466459513152941 1104 8 0.22739556910227066 1104 9 0.2690324341261672 1104 10-11 0.2196207429058319 1104 12-13 0.25838004824989724 1104 14-15 0.3690208943640485 1104 16-17 0.3327772245697247 1104 18-19 0.3669266421261028 1104 20-21 0.41521962141516155 1104 22-23 0.28842983891116347 1104 24-25 0.278522793355215 1104 26-27 0.3453127185550997 1104 28-29 0.28428534300909547 1104 30-31 0.2161032980897275 1104 32-33 0.30241435561727315 1104 34-35 0.3919314175457913 1104 36-37 0.21222319929945144 1104 38-39 0.33544703767250184 1104 40-41 0.38490532793497323 1104 42-43 0.3859889911579728 1104 44-45 0.45050645270511325 1104 46-47 0.46757728453507497 1104 48-49 0.4612362709641431 1104 50-51 0.4736841534388532 1104 52-53 0.5859965987295936 1104 54-55 0.3763210585356447 1104 56-57 0.2526870700972168 1104 58-59 0.38375676845956974 1104 60-61 0.24544267415205923 1104 62-63 0.2631301704851481 1104 64-65 0.43028407327381757 1104 66-67 0.33431890936110875 1104 68-69 0.2768343410711367 1104 70-71 0.3489595602580309 1104 72-73 0.13058190715510776 1104 74-75 0.08373342807239936 1104 76-77 0.14067595377334996 1104 78-79 0.0860668062489296 1104 80-81 0.01894396401139531 1104 82-83 0.10455922593272504 1104 84-85 0.1796982043275115 1104 86-87 0.06877419755866043 1104 88-89 0.20237157004840967 1104 90-91 0.2741909499778856 1104 92-93 0.27202684372639396 1104 94-95 0.26714647022928517 1104 96-97 0.15910416233036173 1104 98-99 0.3132385167278606 1104 100-101 0.6687921419134817 1105 1 0.07723013666934264 1105 2 0.10744259073358364 1105 3 0.1802124555124216 1105 4 0.2610128889317025 1105 5 0.18279518699755926 1105 6 0.17224221408565654 1105 7 0.21344170964606946 1105 8 0.1987035174174565 1105 9 0.26278397630513695 1105 10-11 0.32882557035230064 1105 12-13 0.34659444071665035 1105 14-15 0.37940113802331865 1105 16-17 0.45158568945218036 1105 18-19 0.4054279551097011 1105 20-21 0.4759736992874224 1105 22-23 0.4221816418813731 1105 24-25 0.46044775368485347 1105 26-27 0.4985070100354534 1105 28-29 0.6090885459267454 1105 30-31 0.5663043079536507 1105 32-33 0.4799444820207768 1105 34-35 0.41537178562253985 1105 36-37 0.6300205464507158 1105 38-39 0.6711269906498316 1105 40-41 0.602848567724493 1105 42-43 0.5823397192670541 1105 44-45 0.605040809944974 1105 46-47 0.7799364823412205 1105 48-49 0.7014247528180846 1105 50-51 0.7076485012701781 1105 52-53 0.8048681550314072 1105 54-55 0.6029403715312753 1105 56-57 0.5910112957979976 1105 58-59 0.6071169432298262 1105 60-61 0.608042802942812 1105 62-63 0.6160168286058862 1105 64-65 0.5802911948807719 1105 66-67 0.41112819874510365 1105 68-69 0.5656819547309766 1105 70-71 0.5167348404581524 1105 72-73 0.4947395113261628 1105 74-75 0.6583224812597734 1105 76-77 0.5957769802877912 1105 78-79 0.6184405281210914 1105 80-81 0.7396365453030391 1105 82-83 0.7444875183719866 1105 84-85 0.7081971701899406 1105 86-87 0.6762099075026313 1105 88-89 0.7775880267781261 1105 90-91 0.9154502807875602 1105 92-93 0.8677001164357918 1105 94-95 0.8286237422726614 1105 96-97 0.9290426557193037 1105 98-99 0.9179206222359717 1105 100-101 1.1797391049994808 1106 1 0.08895332535570333 1106 2 0.06499702865632173 1106 3 0.0610188151445179 1106 4 0.051132942743166154 1106 5 0.1525468740105822 1106 6 0.06385615187098637 1106 7 0.08824873618919327 1106 8 0.018967228973849615 1106 9 0.0747221913878775 1106 10-11 0.05418401046183163 1106 12-13 0.10941327479500984 1106 14-15 0.2805363689740048 1106 16-17 0.2824176969886665 1106 18-19 0.17732563143576385 1106 20-21 0.273062621194299 1106 22-23 0.2951167480974419 1106 24-25 0.13325550478946724 1106 26-27 0.11256026006386577 1106 28-29 0.008196347704803486 1106 30-31 0.03415746043486223 1106 32-33 0.12336867706802224 1106 34-35 0.1345251443679274 1106 36-37 0.3330294829274365 1106 38-39 0.3285701566297732 1106 40-41 0.4680181403347632 1106 42-43 0.428844463474249 1106 44-45 0.3808818925158377 1106 46-47 0.3927375545087841 1106 48-49 0.5146799615864595 1106 50-51 0.350527719294071 1106 52-53 0.19328666856011267 1106 54-55 0.1736871104313522 1106 56-57 0.18650654274669876 1106 58-59 0.3060955473745679 1106 60-61 0.33550114475174553 1106 62-63 0.2506449880395465 1106 64-65 0.2611350436911195 1106 66-67 0.29738240419890616 1106 68-69 0.31374505479939785 1106 70-71 0.3755900087401862 1106 72-73 0.3529535638883132 1106 74-75 0.18094451434917147 1106 76-77 0.28299321144780265 1106 78-79 0.24749297071547716 1106 80-81 0.25679828531190196 1106 82-83 0.1901789406711174 1106 84-85 0.3820807902851904 1106 86-87 0.4255096263583589 1106 88-89 0.6057630768374018 1106 90-91 0.6703084377219461 1106 92-93 0.6920642171000502 1106 94-95 0.8384941319144481 1106 96-97 0.9023034870455149 1106 98-99 0.8921288373612946 1106 100-101 1.3351169677461776 1107 1 0.007748845669468096 1107 2 0.07507967931270443 1107 3 0.14614652215685098 1107 4 0.13706204611522566 1107 5 0.10364878593637883 1107 6 0.07572104606278174 1107 7 0.04027065630001658 1107 8 0.024890558289982323 1107 9 0.06374858034041608 1107 10-11 0.07826576128210405 1107 12-13 0.02018518925765278 1107 14-15 0.23050356110451276 1107 16-17 0.13608431126171894 1107 18-19 0.19467519291089985 1107 20-21 0.15418125658058557 1107 22-23 0.13296106935055008 1107 24-25 0.09662454216493899 1107 26-27 0.15727671647830022 1107 28-29 0.11985937586631934 1107 30-31 0.006698701847206223 1107 32-33 0.06926893598068773 1107 34-35 0.1666541427160837 1107 36-37 0.09686349479571987 1107 38-39 0.03083890230083597 1107 40-41 0.10747022470762602 1107 42-43 0.055043370825337945 1107 44-45 0.3563131473611705 1107 46-47 0.3557941667226743 1107 48-49 0.22132205435737262 1107 50-51 0.19741873390768916 1107 52-53 0.18633407907837807 1107 54-55 0.12477276366136891 1107 56-57 0.26300552187811377 1107 58-59 0.2086161132126776 1107 60-61 0.20899848623309225 1107 62-63 0.1557359608888973 1107 64-65 0.17052081356417403 1107 66-67 0.041941389294798626 1107 68-69 0.15627373626839614 1107 70-71 0.21014111704262106 1107 72-73 0.10193906968464361 1107 74-75 0.16674127274446704 1107 76-77 0.0710110852009258 1107 78-79 0.11874596667266957 1107 80-81 0.13070649170099458 1107 82-83 0.03271048657534692 1107 84-85 0.34734463139762894 1107 86-87 0.21834636775101401 1107 88-89 0.3073094603547766 1107 90-91 0.40926668539118083 1107 92-93 0.6205875611982776 1107 94-95 0.6203771922738284 1107 96-97 0.6115281304192948 1107 98-99 0.8507320596653507 1107 100-101 1.4405479127483787 1108 1 0.22789843699447943 1108 2 0.22850641508977532 1108 3 0.03208876969812735 1108 4 0.20276434949256839 1108 5 0.2898301287825191 1108 6 0.2712085741222907 1108 7 0.07569829612945966 1108 8 0.2359282762773418 1108 9 0.17421623531658526 1108 10-11 0.343002024611458 1108 12-13 0.34853082056776685 1108 14-15 0.4541182789933558 1108 16-17 0.5585496372899783 1108 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quality scores pass #Quality Count 2 17997.0 3 4546.0 4 4256.0 5 4769.0 6 5565.0 7 6523.0 8 8226.0 9 9438.0 10 11494.0 11 13929.0 12 17475.0 13 20994.0 14 23016.0 15 25910.0 16 30881.0 17 37770.0 18 33737.0 19 24800.0 20 24835.0 21 26102.0 22 27945.0 23 30989.0 24 35006.0 25 39978.0 26 45773.0 27 53008.0 28 61863.0 29 73126.0 30 86392.0 31 103571.0 32 127615.0 33 160426.0 34 211613.0 35 296457.0 36 424363.0 37 617764.0 38 561866.0 39 91867.0 40 51.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.631054687845435 18.87324043303166 14.149138057583095 22.346566821539806 2 15.215250171441056 20.737227457304623 40.61031543682062 23.437206934433696 3 19.612485821807923 25.582185799334876 29.686240401463373 25.11908797739383 4 12.419177036738674 16.605364878138403 37.68682029212864 33.28863779299429 5 13.248114593579038 38.128525284217986 33.84025331658857 14.783106805614407 6 30.878732391172004 36.246890134226035 17.98027416594335 14.894103308658616 7 28.104113515803963 33.39108839412053 20.8605386189822 17.6442594710933 8 26.149780353620798 34.24547409404525 20.156986676230304 19.447758876103645 9 27.009424041845904 17.10664369632518 20.804870638745847 35.07906162308308 10-11 24.864564332586376 26.82191036348145 27.169139387209114 21.144385916723063 12-13 26.41081656225338 24.40518010076346 27.69273301807562 21.49127031890754 14-15 23.02610598635293 25.419171912235456 26.94048746761302 24.61423463379859 16-17 21.777433703912088 28.946592998041147 27.265832782164935 22.010140515881833 18-19 21.474518761041715 27.287630806364742 30.150579796744086 21.08727063584946 20-21 22.602646824880797 26.15094085651351 29.972361838277212 21.274050480328484 22-23 22.054648370684447 26.316345490954678 29.879662430670678 21.749343707690198 24-25 22.114618971201548 26.394927207962287 30.007370469124538 21.483083351711628 26-27 21.788585942296837 26.5598435912423 30.18953913190839 21.462031334552474 28-29 21.361340674445106 27.011201137586276 30.21651620395457 21.410941984014045 30-31 21.57999134406402 26.612787199592443 30.044043265528504 21.76317819081504 32-33 21.35848414217293 26.805921496348837 29.980034505934277 21.85555985554395 34-35 22.206553101600807 26.59857974828443 29.696625500805418 21.498241649309346 36-37 22.094523737632667 26.381757224416695 29.815198023411043 21.708521014539595 38-39 21.75149103783788 26.766368218105395 29.967684220745898 21.51445652331083 40-41 21.853050675018945 26.66117609370709 29.971802800723175 21.513970430550792 42-43 21.60017468898279 26.836639243643916 29.466401879086895 22.096784188286396 44-45 21.73123626913173 26.954163818250045 29.26409997584533 22.050499936772898 46-47 22.02251773737747 27.186717134997966 28.772792314238405 22.01797281338616 48-49 22.145637230961825 27.200261413252115 28.626699307901333 22.027402047884735 50-51 21.779052737401543 27.417285862961975 28.742893287296255 22.060768112340224 52-53 22.221917224369996 27.54204395392919 28.407008024198333 21.82903079750248 54-55 21.661686430939607 27.20734578989923 28.698037158967978 22.43293062019319 56-57 21.97305884050504 27.60695745367063 28.27994054779944 22.14004315802488 58-59 22.316619139610072 27.06906421529027 28.3678044004356 22.246512244664064 60-61 22.013425292779758 27.1286019190575 28.968788590344257 21.88918419781848 62-63 21.91912122844581 27.197153902030596 28.914849159965232 21.968875709558365 64-65 21.64027314958045 27.27221200148312 28.732278694757824 22.355236154178606 66-67 21.68781743871462 27.507241824384725 28.306543798719204 22.49839693818145 68-69 21.676847148985193 27.29752442968854 28.596150385644908 22.42947803568136 70-71 21.777675167537954 27.307763504522836 28.38077695568212 22.53378437225709 72-73 21.83238451230048 27.157010015942674 28.52908951114991 22.48151596060694 74-75 21.615082629807304 27.654631818180476 28.263237551065227 22.467048000946992 76-77 21.8263375496143 27.282774363248702 28.30820545574322 22.58268263139378 78-79 21.82184203964869 27.36995852777282 28.339547036528312 22.468652396050178 80-81 21.86956132329999 27.69040154669971 28.119866571623465 22.320170558376834 82-83 21.935857833194596 27.467892098740325 28.162591197979282 22.433658870085793 84-85 21.74777468213961 27.42192203713979 28.061450057256188 22.768853223464415 86-87 21.89970632408116 27.340192165749965 28.21834248260772 22.541759027561152 88-89 21.918628966350163 27.273026442973897 27.98515358930912 22.82319100136682 90-91 21.86163853984052 27.28378152782446 28.16995626027975 22.684623672055274 92-93 22.137068134356955 27.30854795371391 28.112426028515813 22.441957883413323 94-95 21.8661208380866 27.158322763956892 28.059007824881377 22.916548573075133 96-97 21.96135139192589 26.918233404715664 28.16727408319567 22.95314112016278 98-99 21.96975176537587 27.02029588109962 27.80456294935196 23.205389404172543 100-101 22.225775461203696 26.429668742040263 28.36248153488118 22.98207426187486 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5504.0 1 3635.0 2 1989.5 3 2216.0 4 1894.5 5 1787.5 6 2749.0 7 4940.0 8 7412.5 9 8104.5 10 6671.5 11 5270.0 12 5117.5 13 5386.0 14 5587.0 15 5802.5 16 6110.5 17 6466.5 18 6741.0 19 7206.0 20 7978.0 21 9013.5 22 10420.0 23 12158.5 24 14187.5 25 17076.0 26 21326.5 27 26257.0 28 30350.5 29 34780.5 30 40862.5 31 47319.5 32 55420.5 33 63098.5 34 69465.5 35 75522.5 36 82866.0 37 90738.0 38 93879.5 39 94420.0 40 95821.0 41 99909.5 42 103768.0 43 107890.5 44 112952.0 45 117058.0 46 118107.0 47 123727.5 48 147632.0 49 149376.5 50 129380.5 51 119779.0 52 112377.5 53 110457.5 54 107806.5 55 99159.0 56 89330.5 57 79540.5 58 70976.5 59 62206.0 60 52819.0 61 43213.0 62 35165.5 63 28099.5 64 21233.0 65 16821.5 66 12828.0 67 10163.5 68 7611.0 69 4997.5 70 3633.0 71 2826.0 72 2247.0 73 1880.0 74 1538.0 75 1300.5 76 1003.0 77 667.0 78 430.0 79 297.0 80 225.5 81 180.5 82 134.5 83 93.5 84 68.0 85 51.5 86 39.0 87 27.0 88 19.5 89 13.5 90 13.5 91 12.0 92 11.0 93 12.5 94 8.5 95 5.0 96 3.5 97 1.5 98 3.5 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27872364441894265 2 0.16828652861194332 3 0.1986221963023408 4 0.16531763090193347 5 0.24215623104020767 6 0.19674091458510684 7 0.2438023525427874 8 0.2506513937945922 9 0.14967947662742626 10-11 0.20748479689212262 12-13 0.21320212961090393 14-15 0.173283683173346 16-17 0.290996068121211 18-19 0.23480747433226257 20-21 0.20022422526467282 22-23 0.24810872397364325 24-25 0.23476338179201492 26-27 0.3481693953090241 28-29 0.32030290987249616 30-31 0.29524364949840326 32-33 0.19336048649945206 34-35 0.36438075260675096 36-37 0.4079147873446179 38-39 0.38769100888435293 40-41 0.3493011038420476 42-43 0.38378147031572607 44-45 0.3926440709055079 46-47 0.39802336081572376 48-49 0.4173064984173718 50-51 0.40982546408868364 52-53 0.4095903038740294 54-55 0.40299112035029466 56-57 0.38198837367898747 58-59 0.3560472624999412 60-61 0.3218314512677487 62-63 0.39085097426876925 64-65 0.4258751487388358 66-67 0.43116625356855626 68-69 0.43556081007990743 70-71 0.43484063192252886 72-73 0.4258310561985881 74-75 0.42202440022387255 76-77 0.40565137027857073 78-79 0.3798131416934357 80-81 0.3909979494029282 82-83 0.4007864933379111 84-85 0.4014184864147944 86-87 0.3970386274168591 88-89 0.40497528466143984 90-91 0.42656593186938263 92-93 0.4256987785778451 94-95 0.434811236895697 96-97 0.4343556139798045 98-99 0.4320187093466779 100-101 0.40538681503708474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3401936.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.78316333237659 #Duplication Level Percentage of deduplicated Percentage of total 1 72.83559871488748 34.07479711090019 2 14.313087256590581 13.392229978312702 3 5.300735712729124 7.439555538911049 4 2.488757336295876 4.657277638343219 5 1.3753330595695894 3.217121558113066 6 0.8235083015457522 2.311579402606977 7 0.5299135547278434 1.7353722668011087 8 0.4004878467495414 1.498887067769248 9 0.2749513956244576 1.1576786444967504 >10 1.4178377575394614 12.293571397715752 >50 0.13792672215745594 4.462757953950397 >100 0.09288602569258828 8.061614568553882 >500 0.005562787313423822 1.767594425554899 >1k 0.003160674608110069 2.4747209145123636 >5k 1.264269843244028E-4 0.5169112088139675 >10k+ 1.264269843244028E-4 0.9383303246443373 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18135 0.5330788115943392 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13594 0.399595994751224 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9163 0.26934663085960464 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8316 0.24444904313308657 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4909 0.14430018671721043 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3792 0.11146594174611163 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3491 0.10261803866974571 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.818508049534148E-5 0.0 10-11 0.0 0.0 0.0 1.9106767440657318E-4 0.0 12-13 0.0 0.0 0.0 3.2334529514958544E-4 0.0 14-15 0.0 0.0 0.0 3.2334529514958544E-4 0.0 16-17 0.0 0.0 0.0 3.5274032198136593E-4 0.0 18-19 0.0 0.0 0.0 4.409254024767074E-4 0.0 20-21 0.0 0.0 0.0 4.997154561402683E-4 0.0 22-23 2.9395026831780493E-5 0.0 0.0 7.054806439627317E-4 0.0 24-25 2.9395026831780493E-5 0.0 0.0 0.0013815662610936832 0.0 26-27 2.9395026831780493E-5 0.0 0.0 0.002042954364808744 0.0 28-29 2.9395026831780493E-5 0.0 0.0 0.0045709266723418665 0.0 30-31 2.9395026831780493E-5 0.0 0.0 0.013595199909698477 0.0 32-33 2.9395026831780493E-5 0.0 0.0 0.03205527676005663 0.0 34-35 2.9395026831780493E-5 0.0 0.0 0.06034799008564534 0.0 36-37 2.9395026831780493E-5 0.0 0.0 0.10685092253352209 0.0 38-39 2.9395026831780493E-5 0.0 0.0 0.17610560574919693 0.0 40-41 2.9395026831780493E-5 0.0 0.0 0.2537966616655928 0.0 42-43 2.9395026831780493E-5 0.0 0.0 0.3306499593172829 0.0 44-45 2.9395026831780493E-5 0.0 0.0 0.4269333697047799 0.0 46-47 2.9395026831780493E-5 0.0 0.0 0.5472619120406733 0.0 48-49 2.9395026831780493E-5 0.0 0.0 0.6764971475065962 0.0 50-51 2.9395026831780493E-5 0.0 0.0 0.8164468702527031 0.0 52-53 2.9395026831780493E-5 0.0 0.0 0.9542801510669219 0.0 54-55 2.9395026831780493E-5 0.0 0.0 1.1043123680163296 0.0 56-57 2.9395026831780493E-5 0.0 0.0 1.2866497194538637 0.0 58-59 2.9395026831780493E-5 0.0 0.0 1.4923708147360797 0.0 60-61 2.9395026831780493E-5 0.0 0.0 1.7188594964749484 0.0 62-63 2.9395026831780493E-5 0.0 0.0 1.9463623066395135 0.0 64-65 2.9395026831780493E-5 0.0 0.0 2.176716434406761 0.0 66-67 2.9395026831780493E-5 0.0 0.0 2.445210609488244 0.0 68-69 2.9395026831780493E-5 0.0 0.0 2.7504926606497007 0.0 70-71 2.9395026831780493E-5 0.0 0.0 3.08934383245305 0.0 72-73 2.9395026831780493E-5 0.0 0.0 3.4278716589612506 0.0 74-75 2.9395026831780493E-5 0.0 0.0 3.768560019941586 0.0 76-77 2.9395026831780493E-5 0.0 0.0 4.1320736192568 0.0 78-79 2.9395026831780493E-5 0.0 0.0 4.558521971018855 0.0 80-81 2.9395026831780493E-5 0.0 0.0 5.024051010953762 0.0 82-83 2.9395026831780493E-5 0.0 0.0 5.490300229046049 0.0 84-85 2.9395026831780493E-5 0.0 0.0 5.961384929052164 0.0 86-87 2.9395026831780493E-5 0.0 0.0 6.448107783332785 0.0 88-89 2.9395026831780493E-5 0.0 0.0 6.973000079954472 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2575 0.0 26.52757 2 GTACATG 19105 0.0 26.343199 1 TACATGG 19310 0.0 25.57294 2 ACATGGG 19315 0.0 24.240469 3 GTATCAA 29470 0.0 23.915564 1 GAGTACT 14900 0.0 22.663897 12-13 GTATAGG 1175 0.0 22.245615 1 GGTATCA 22100 0.0 21.203337 1 GTACTTT 16165 0.0 20.993551 14-15 CATGGGG 7385 0.0 20.8762 4 ATCAACG 34110 0.0 20.568615 3 TCAACGC 34225 0.0 20.485641 4 TATCAAC 34260 0.0 20.422874 2 AGTACTT 15520 0.0 20.367065 12-13 CAACGCA 34425 0.0 20.352846 5 GTACCTG 3450 0.0 20.249664 1 AACGCAG 35085 0.0 20.008772 6 CATGGGA 11205 0.0 19.643766 4 CGTATAG 275 2.8252543E-8 19.00989 1 ACGCAGA 37795 0.0 18.449677 7 >>END_MODULE