FastQCFastQC Report
Thu 26 May 2016
SRR938708_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938708_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3401936
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173190.5090924696996063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145790.4285500961805278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100080.29418542853245916No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT92110.27075759214753015No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44110.12966146335498377No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41450.12184238621773015No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36710.10790914349946619No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35130.10326472926004487No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG189800.024.438631
TACATGG193100.023.5185892
ACATGGG193050.022.8609753
GAGTACT155350.021.4097612-13
GTATCAA317800.020.8528611
CATGGGG73000.020.477144
GTACTTT162700.020.4717314-15
AGTACTT158600.020.19322212-13
TACCTGG27550.019.9810352
GGTATCA249100.019.6903461
GTATAGG10400.019.2125231
CATGGGA114000.019.0652374
ATCAACG368550.017.8978143
TCAACGC369050.017.834994
GTGTAGC16900.017.7346361
CAACGCA372500.017.7080255
TATCAAC373350.017.693132
CATTCCG5100.017.6741039
ACTTTTT190500.017.65756216-17
AACGCAG379750.017.3781056
Produced by FastQC (version 0.11.5)