##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938708_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3401936 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08590579011481 33.0 31.0 34.0 30.0 34.0 2 32.285383969598485 34.0 31.0 34.0 30.0 34.0 3 32.35900058084573 34.0 31.0 34.0 30.0 34.0 4 35.97288161799634 37.0 35.0 37.0 35.0 37.0 5 35.883186221022385 37.0 35.0 37.0 35.0 37.0 6 35.91687791892616 37.0 35.0 37.0 35.0 37.0 7 35.83969480907342 37.0 35.0 37.0 35.0 37.0 8 35.82505667361173 37.0 35.0 37.0 33.0 37.0 9 37.472231106052554 39.0 37.0 39.0 34.0 39.0 10-11 37.40000576142526 39.0 37.0 39.0 34.0 39.0 12-13 37.31935198075449 39.0 37.0 39.0 34.0 39.0 14-15 38.52824053715296 40.0 38.0 41.0 34.0 41.0 16-17 38.460059801242586 40.0 38.0 41.0 34.0 41.0 18-19 38.621773307904675 40.0 38.0 41.0 34.0 41.0 20-21 38.631909154081676 40.0 38.0 41.0 34.0 41.0 22-23 38.556355998466756 40.0 38.0 41.0 34.0 41.0 24-25 38.474973221130554 40.0 38.0 41.0 34.0 41.0 26-27 38.29576805677708 40.0 38.0 41.0 33.5 41.0 28-29 38.126038232347696 40.0 38.0 41.0 33.0 41.0 30-31 37.894966278025215 40.0 37.0 41.0 33.0 41.0 32-33 37.723835339641894 40.0 37.0 41.0 33.0 41.0 34-35 37.67504988336053 40.0 37.0 41.0 33.0 41.0 36-37 37.554218539090684 40.0 37.0 41.0 32.5 41.0 38-39 37.42149249721335 40.0 36.0 41.0 32.0 41.0 40-41 37.242162844921246 40.0 36.0 41.0 31.5 41.0 42-43 37.0245623374455 40.0 36.0 41.0 31.0 41.0 44-45 36.757474861373055 40.0 36.0 41.0 30.5 41.0 46-47 36.40765816876038 39.0 35.0 41.0 30.0 41.0 48-49 36.06893956852804 39.0 35.0 41.0 29.0 41.0 50-51 35.82980808574882 39.0 35.0 41.0 28.0 41.0 52-53 35.60563587909943 39.0 35.0 40.5 28.0 41.0 54-55 35.334942221135265 38.5 35.0 40.0 27.0 41.0 56-57 35.07099486880412 38.0 34.5 40.0 26.5 41.0 58-59 34.775802807577804 38.0 34.0 40.0 26.0 41.0 60-61 34.413404602555715 38.0 34.0 40.0 26.0 41.0 62-63 34.063380528028745 37.0 33.5 40.0 25.0 41.0 64-65 33.670552738205544 36.5 33.0 39.5 23.5 41.0 66-67 33.2689305442548 36.0 33.0 39.0 23.0 40.5 68-69 32.92858184280951 36.0 32.0 39.0 22.0 40.0 70-71 32.494620857064916 35.0 32.0 38.5 21.5 40.0 72-73 32.01352303511882 35.0 31.5 37.5 20.0 39.5 74-75 31.526471103512822 35.0 31.0 37.0 18.5 39.0 76-77 29.587036616797022 33.0 28.5 35.0 14.0 37.5 78-79 30.535546818047138 34.0 30.5 36.0 13.5 38.0 80-81 30.580936266878624 34.0 31.0 36.0 14.0 37.0 82-83 30.25632698557527 34.0 30.5 35.0 9.0 37.0 84-85 29.853819119466092 34.0 30.0 35.0 6.0 36.5 86-87 29.27739881055963 34.0 29.0 35.0 2.0 36.0 88-89 28.677949702757488 34.0 29.0 35.0 2.0 36.0 90-91 28.16002050009171 33.5 28.0 35.0 2.0 35.0 92-93 27.865335356103113 33.0 27.0 35.0 2.0 35.0 94-95 27.52957169094304 33.0 27.0 35.0 2.0 35.0 96-97 26.920947954341294 33.0 26.0 35.0 2.0 35.0 98-99 26.096675246095167 33.0 24.5 35.0 2.0 35.0 100-101 22.39647909308112 28.5 9.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.062057427801953224 1101 2 0.17391490024949263 1101 3 0.07272428354493599 1101 4 0.11656457522905583 1101 5 -0.029061896598399528 1101 6 0.11156767974976134 1101 7 0.2257247448298827 1101 8 0.19515151291107458 1101 9 0.18702608311821933 1101 10-11 0.2543924393500774 1101 12-13 0.21263670088553255 1101 14-15 0.27987428040204776 1101 16-17 0.3512746050858411 1101 18-19 0.32338955046996176 1101 20-21 0.31380119048417754 1101 22-23 0.24985024417679114 1101 24-25 0.26612880387192916 1101 26-27 0.18808116139783237 1101 28-29 0.2551150915413629 1101 30-31 0.25017270692335103 1101 32-33 0.3754598413219128 1101 34-35 0.3305637932854282 1101 36-37 0.4382709299232488 1101 38-39 0.46331611274850104 1101 40-41 0.5268006087631676 1101 42-43 0.6074832410806437 1101 44-45 0.5879079589068823 1101 46-47 0.48059171477089535 1101 48-49 0.32012546790132745 1101 50-51 0.4254506830528655 1101 52-53 0.27929047176106536 1101 54-55 0.3608166173384362 1101 56-57 0.31636091789916065 1101 58-59 0.24794997918623807 1101 60-61 0.4691871877205145 1101 62-63 0.4240418031979374 1101 64-65 0.3398694543652354 1101 66-67 0.34614788698835497 1101 68-69 0.3588502158526694 1101 70-71 0.3634718300736921 1101 72-73 0.21444368123470525 1101 74-75 0.182931154030058 1101 76-77 0.1807763387654724 1101 78-79 0.07559459853770178 1101 80-81 0.32451690126348254 1101 82-83 0.4472228095809321 1101 84-85 0.45167226282005046 1101 86-87 0.491656913950294 1101 88-89 0.5946498969209735 1101 90-91 0.47494112364984886 1101 92-93 0.512358336320009 1101 94-95 0.5001549431091128 1101 96-97 0.4558884272616872 1101 98-99 0.30095003600983006 1101 100-101 0.8769126741860624 1102 1 0.21889079622063434 1102 2 0.18843705764104612 1102 3 0.1929608514827521 1102 4 0.126265343532026 1102 5 0.0015886652305923121 1102 6 0.08511888648720856 1102 7 0.0971958536990627 1102 8 0.1551695719365469 1102 9 0.1586231020550386 1102 10-11 0.1852602655544615 1102 12-13 0.196178096964708 1102 14-15 0.23964727156716492 1102 16-17 0.28269651502638027 1102 18-19 0.28765558480593967 1102 20-21 0.30098960940591013 1102 22-23 0.2084922954914319 1102 24-25 0.2419448030563629 1102 26-27 0.1501901748904686 1102 28-29 0.2865054908656077 1102 30-31 0.21716626313471465 1102 32-33 0.3157022082723131 1102 34-35 0.3265173270421755 1102 36-37 0.32667263996997065 1102 38-39 0.4045296157095706 1102 40-41 0.3700044440553185 1102 42-43 0.4729045764158286 1102 44-45 0.44834081944858184 1102 46-47 0.49550259136181296 1102 48-49 0.4898931499247894 1102 50-51 0.43341691108646074 1102 52-53 0.3276358334115841 1102 54-55 0.3646515123008385 1102 56-57 0.34302790231993896 1102 58-59 0.32031728705423035 1102 60-61 0.3405044506395356 1102 62-63 0.20110525207461194 1102 64-65 0.29977073875384264 1102 66-67 0.3960396519932985 1102 68-69 0.3457587463590528 1102 70-71 0.3768614588442105 1102 72-73 0.3458571177322085 1102 74-75 0.24522234654800457 1102 76-77 0.23189238389185363 1102 78-79 0.09995680645216609 1102 80-81 0.24476472299283714 1102 82-83 0.2967592195684752 1102 84-85 0.42627364690401137 1102 86-87 0.519904327895194 1102 88-89 0.2934960522947918 1102 90-91 0.38566360382662523 1102 92-93 0.4518559670658959 1102 94-95 0.5865112434450062 1102 96-97 0.5536592497549719 1102 98-99 0.8402891868914004 1102 100-101 1.6359946825509368 1103 1 0.20497040183970228 1103 2 0.17392487412057278 1103 3 0.14613433694452027 1103 4 0.11302253864332101 1103 5 -0.049557676732284506 1103 6 -0.02560296924740868 1103 7 0.0958728174449135 1103 8 0.09932702154569029 1103 9 0.1301503457407449 1103 10-11 0.1815922356731292 1103 12-13 0.23429548688169177 1103 14-15 0.3135091921004829 1103 16-17 0.23813074905604736 1103 18-19 0.07463871220546281 1103 20-21 0.19798473070971312 1103 22-23 0.25050025185404934 1103 24-25 0.20768428156129914 1103 26-27 0.2341097890325443 1103 28-29 0.11388140242954847 1103 30-31 0.23520494483691579 1103 32-33 0.2444222043944393 1103 34-35 0.27903720045166125 1103 36-37 0.3424767538765465 1103 38-39 0.26820685292784674 1103 40-41 0.2871031582683443 1103 42-43 0.3020434130511802 1103 44-45 0.2605921507149205 1103 46-47 0.27017479383613363 1103 48-49 0.36051334649831546 1103 50-51 0.4310227961093247 1103 52-53 0.3722270025074934 1103 54-55 0.3084885714691268 1103 56-57 0.29066389523091374 1103 58-59 0.31508476161804566 1103 60-61 0.3459512177418773 1103 62-63 0.35618640858041317 1103 64-65 0.4220730499457588 1103 66-67 0.32970911015240745 1103 68-69 0.2566055699185341 1103 70-71 0.38584768490662924 1103 72-73 0.43720248022314223 1103 74-75 0.5346500063524644 1103 76-77 0.4605355485199176 1103 78-79 0.3063181897612246 1103 80-81 0.5542736831677324 1103 82-83 0.6758301028429266 1103 84-85 0.7349490994231083 1103 86-87 0.6223307670535405 1103 88-89 0.49783996084561366 1103 90-91 0.515720607921974 1103 92-93 0.540199872506129 1103 94-95 0.6420030751736654 1103 96-97 0.7092141227095361 1103 98-99 0.8171672879196272 1103 100-101 1.4908233371728947 1104 1 0.09529599648930542 1104 2 0.0950440131529291 1104 3 0.0726724509485166 1104 4 0.023747578325924223 1104 5 -0.07862066507684773 1104 6 -0.03760077357080149 1104 7 -0.03972097623221771 1104 8 0.01484318749660929 1104 9 -0.010401658839221284 1104 10-11 0.052760628795596176 1104 12-13 0.020323239331965226 1104 14-15 0.012794392758365802 1104 16-17 0.0833049245369395 1104 18-19 0.08822702386648729 1104 20-21 0.0734624723199957 1104 22-23 -0.002394002535901052 1104 24-25 -0.006067065750762879 1104 26-27 0.05142465182581191 1104 28-29 0.04943209237121238 1104 30-31 -0.16331253780242605 1104 32-33 -0.041765072879428544 1104 34-35 -0.12151579331034412 1104 36-37 0.04993692904889713 1104 38-39 0.16677148828613042 1104 40-41 0.07664314761797897 1104 42-43 0.08919693666623374 1104 44-45 0.3222029439722931 1104 46-47 0.2249746843598217 1104 48-49 0.245623440409112 1104 50-51 0.10973480421548487 1104 52-53 0.12014267294484426 1104 54-55 0.17960148326740466 1104 56-57 0.17418489126034586 1104 58-59 0.31490473561230203 1104 60-61 0.4004709083585354 1104 62-63 0.339951138039126 1104 64-65 0.15364671556225318 1104 66-67 0.15829975126025175 1104 68-69 0.20510570343248702 1104 70-71 0.19852453963576266 1104 72-73 0.2482842148486739 1104 74-75 0.2835371765589123 1104 76-77 0.21760627811086763 1104 78-79 0.07063044941689256 1104 80-81 0.22179909310168355 1104 82-83 0.31222062371133674 1104 84-85 0.3404497203526624 1104 86-87 0.4688209902960381 1104 88-89 0.4929023792573126 1104 90-91 0.5215731828960806 1104 92-93 0.5847171644844771 1104 94-95 0.5164004044846671 1104 96-97 0.5395145624086375 1104 98-99 0.7529979471300159 1104 100-101 1.364638689175628 1105 1 0.15260186248674046 1105 2 0.16608354709892126 1105 3 0.07998212851023823 1105 4 0.10238867541448826 1105 5 0.042916077399823394 1105 6 0.013189323583965518 1105 7 0.10354669789107618 1105 8 0.14676493808789814 1105 9 0.10778692532513645 1105 10-11 0.11291839746574084 1105 12-13 0.06483930106326596 1105 14-15 0.18247420129747383 1105 16-17 0.12970846287224447 1105 18-19 0.16148854933475576 1105 20-21 0.06412338753676039 1105 22-23 0.043513530920613164 1105 24-25 0.07449385608608594 1105 26-27 0.17521498168660798 1105 28-29 0.24784633117173627 1105 30-31 0.17076981806528124 1105 32-33 0.3046130884620766 1105 34-35 0.28253434480870965 1105 36-37 0.2781357843759622 1105 38-39 0.3547437962685507 1105 40-41 0.2319867099862094 1105 42-43 0.3038283784606364 1105 44-45 0.3284581723351394 1105 46-47 0.25686920232733 1105 48-49 0.31619104251389274 1105 50-51 0.25497120144468255 1105 52-53 0.2698002339850305 1105 54-55 0.32107170391685 1105 56-57 0.30465523829324326 1105 58-59 0.4524540602632925 1105 60-61 0.40361745498098145 1105 62-63 0.4047687948097689 1105 64-65 0.38386996289465714 1105 66-67 0.36997146323437846 1105 68-69 0.38256361072321 1105 70-71 0.38086225225584514 1105 72-73 0.48265404290699365 1105 74-75 0.5581977467288084 1105 76-77 0.6794748373195034 1105 78-79 0.414025810943766 1105 80-81 0.5244962262035138 1105 82-83 0.5703841418252971 1105 84-85 0.5915382730214596 1105 86-87 0.5725873173322675 1105 88-89 0.43556391435479824 1105 90-91 0.40167641610563365 1105 92-93 0.4996219857120856 1105 94-95 0.799327555460728 1105 96-97 0.996702921891309 1105 98-99 0.9762352491042066 1105 100-101 1.6720265539573766 1106 1 0.12679034215561558 1106 2 0.01700731386218024 1106 3 0.025858744729475802 1106 4 0.028490364278219715 1106 5 -0.016468484265040217 1106 6 -0.007598908908555302 1106 7 -0.053955510678470375 1106 8 -0.0021990241497391594 1106 9 0.020294487853774967 1106 10-11 0.04453295270061375 1106 12-13 0.08729603946871833 1106 14-15 0.07004933115022993 1106 16-17 0.11554434216134979 1106 18-19 -5.119102311397228E-4 1106 20-21 -0.010815211445638795 1106 22-23 0.017466945744182283 1106 24-25 0.02049306142688323 1106 26-27 0.09173879149118846 1106 28-29 0.1416268699480554 1106 30-31 0.10202184688657923 1106 32-33 0.12995237453317543 1106 34-35 0.2150755657079202 1106 36-37 0.05515698173019956 1106 38-39 0.12324435361094288 1106 40-41 0.2206849674682374 1106 42-43 0.19179137963887172 1106 44-45 0.20132821705683313 1106 46-47 0.32951308363158205 1106 48-49 0.21422842097705797 1106 50-51 0.2798102387343775 1106 52-53 0.37201180069858 1106 54-55 0.41688709927618817 1106 56-57 0.3421027346090213 1106 58-59 0.39074194308610544 1106 60-61 0.42354969275900345 1106 62-63 0.4379306671526422 1106 64-65 0.39933007254190755 1106 66-67 0.2501845308252868 1106 68-69 0.2229391445222575 1106 70-71 0.21484308871819024 1106 72-73 0.16207083805885247 1106 74-75 0.3367024198556017 1106 76-77 0.3075773165841724 1106 78-79 0.26004691363332455 1106 80-81 0.2097359636842704 1106 82-83 0.3972870328976974 1106 84-85 0.47704900042943876 1106 86-87 0.4592239893807353 1106 88-89 0.5910534339266427 1106 90-91 0.6034171652537346 1106 92-93 0.7565535529759231 1106 94-95 0.8585798240555391 1106 96-97 0.9586102563075549 1106 98-99 1.0331609039200984 1106 100-101 1.7608106811473974 1107 1 0.04759790633026029 1107 2 0.037662635321211724 1107 3 -0.012174338771309579 1107 4 -0.027519027196312607 1107 5 -0.0444273893826832 1107 6 -0.00454291960846831 1107 7 0.0291205345095662 1107 8 0.03890925247046795 1107 9 -0.05270826652260041 1107 10-11 -0.0608955313079278 1107 12-13 0.07006634048777727 1107 14-15 0.021792029341710872 1107 16-17 -0.041152731628436356 1107 18-19 0.05404997318908755 1107 20-21 0.02376590484171004 1107 22-23 0.07523389012927595 1107 24-25 0.016072834415140846 1107 26-27 -0.038703998555874364 1107 28-29 -0.0037829976521024378 1107 30-31 -0.019349034701058088 1107 32-33 -0.08576517698308805 1107 34-35 -0.06199016008994107 1107 36-37 -0.19090244295564673 1107 38-39 -0.16999010049386953 1107 40-41 -0.285713858097175 1107 42-43 -0.21667139658144663 1107 44-45 -0.19001358768905874 1107 46-47 -0.26444265140347767 1107 48-49 -0.297933728972815 1107 50-51 -0.42889844319937964 1107 52-53 -0.4251737988898512 1107 54-55 -0.36084394203557935 1107 56-57 -0.3204236262639881 1107 58-59 -0.3300221381547672 1107 60-61 -0.33359711350060195 1107 62-63 -0.40500617616548595 1107 64-65 -0.4156600495943863 1107 66-67 -0.2901038718107074 1107 68-69 -0.33026096217996326 1107 70-71 -0.34939106458056557 1107 72-73 -0.36859495835741996 1107 74-75 -0.23622077584067824 1107 76-77 -0.32711843359447457 1107 78-79 -0.13313212373569527 1107 80-81 -0.07749390796966438 1107 82-83 -0.02680381956468736 1107 84-85 -0.06129396655096642 1107 86-87 -0.03155720907960102 1107 88-89 0.01052645369011529 1107 90-91 0.1532760091128047 1107 92-93 0.2854833806063155 1107 94-95 0.2844669194762517 1107 96-97 0.45635739816600207 1107 98-99 0.6719231031866748 1107 100-101 1.2236834853762382 1108 1 0.0736549978666119 1108 2 0.06644952488499456 1108 3 0.03215374560877393 1108 4 -0.02201585288258201 1108 5 -0.06303879916642785 1108 6 -0.005023415761357342 1108 7 -0.06767315361192061 1108 8 0.0038333826344469912 1108 9 0.03801503127044015 1108 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-2.2124418963460606 2316 92-93 -1.8368013280258246 2316 94-95 -1.4993502935333254 2316 96-97 -1.6120165385468717 2316 98-99 -1.767239366338135 2316 100-101 -1.6677614659411368 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 42.0 5 94.0 6 300.0 7 625.0 8 1336.0 9 2436.0 10 4196.0 11 6163.0 12 8795.0 13 12305.0 14 14960.0 15 20091.0 16 26160.0 17 35021.0 18 32804.0 19 22279.0 20 20304.0 21 20776.0 22 22172.0 23 24437.0 24 28308.0 25 33522.0 26 40400.0 27 49157.0 28 59325.0 29 72172.0 30 87477.0 31 105821.0 32 130102.0 33 164228.0 34 218564.0 35 307729.0 36 453987.0 37 704676.0 38 609928.0 39 61222.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19248521956659 19.739646330342133 14.751204017861747 22.316664432229523 2 15.659553854040759 21.456429515428862 39.452270707032696 23.43174592349768 3 19.651545472930707 25.635873220425076 29.90958677647081 24.802994530173407 4 12.93798589979353 17.294740406639043 37.00536988350163 32.761903810065796 5 13.180906936960406 38.3998315760751 33.81874786456223 14.600513622402264 6 30.997680073560364 36.7468175971065 17.411059687838787 14.844442641494348 7 27.96410632063625 33.526556643040905 20.737632924311335 17.771704112011513 8 26.2210106245385 34.72387487595299 19.773652414389925 19.28146208511859 9 26.627426265514696 17.09755856665146 20.608206621171004 35.66680854666284 10-11 24.91526883515739 27.035047102590998 26.926755823742717 21.122928238508894 12-13 26.36213614835788 24.46953734579369 27.590892362466548 21.577434143381886 14-15 22.827825097238748 25.675277253893075 26.78491012176596 24.711987527102213 16-17 21.676372496322927 29.154763117347578 27.204910663302 21.9639537230275 18-19 21.408883059528456 27.379159984197234 30.059545505853137 21.15241145042117 20-21 22.47839171577596 26.277419681028686 29.97250683140423 21.27168177179112 22-23 21.982953236039716 26.425673498854767 29.868698294147805 21.722674970957716 24-25 22.060374123914105 26.54927906909067 30.02137615776498 21.368970649230242 26-27 21.772776049082122 26.679018622145538 30.133538983691565 21.41466634508077 28-29 21.31749392110845 27.128332220241653 30.193528038152394 21.360645820497503 30-31 21.535987155549073 26.65376420955597 30.074610457104423 21.735638177790527 32-33 21.329237234327746 26.900726527483176 29.996184525517233 21.773851712671842 34-35 22.1348867536154 26.672855732637053 29.75584546305992 21.43641205068763 36-37 22.076034159025898 26.462337561536653 29.795978127945084 21.665650151492365 38-39 21.709655649595973 26.866290344938392 30.00002792940094 21.424026076064695 40-41 21.806052335513368 26.742936986955367 29.97213981889265 21.47887085863861 42-43 21.592004319097587 26.952850488287034 29.441005821756676 22.014139370858704 44-45 21.750980952414547 27.004338296593914 29.227972518046354 22.016708232945184 46-47 22.016140612546078 27.247766736122635 28.78044736854869 21.955645282782594 48-49 22.149163000191805 27.32284257319293 28.551206574595096 21.97678785202017 50-51 21.81400344627497 27.479119230414227 28.658830535806985 22.048046787503814 52-53 22.23945424017383 27.619964044003183 28.26101078915065 21.879570926672343 54-55 21.67233598750829 27.366402542552244 28.512176595914795 22.44908487402467 56-57 21.992886882680303 27.714980875303212 28.14998982916525 22.142142412851232 58-59 22.297152009942607 27.20546055461826 28.228675201735015 22.268712233704118 60-61 22.000822495819175 27.302701684176306 28.771773871853508 21.924701948151014 62-63 21.900413210629246 27.334528352857244 28.742860233569683 22.022198202943827 64-65 21.61405903730375 27.39831146456242 28.52809752716363 22.459531970970197 66-67 21.649482566198852 27.630084549390098 28.10772253430929 22.612710350101764 68-69 21.635306240539364 27.44132697791295 28.40287066839059 22.520496113157094 70-71 21.726785157092237 27.44273882992162 28.196486440288602 22.633989572697537 72-73 21.765150784729638 27.213195662705004 28.29988571213568 22.721767840429685 74-75 21.529887188622297 27.710769000130075 28.013289563900358 22.74605424734727 76-77 21.606510504331137 27.29889279248157 28.239196355025847 22.855400348161446 78-79 21.588556454274123 27.36486178191194 28.202748318471947 22.843833445341986 80-81 21.59564207852266 27.660170193277455 28.021777261333895 22.722410466865988 82-83 21.697577574652758 27.411765864404323 28.021175222496954 22.869481338445965 84-85 21.435808904106366 27.346443319333464 27.94314766650519 23.27460011005498 86-87 21.491884692613542 27.281884423356395 28.04714854475341 23.179082339276658 88-89 21.494969502233914 27.166133503379157 27.859990686421366 23.47890630796556 90-91 21.440965548646556 27.14730528177551 28.06075583115471 23.350973338423227 92-93 21.602633105951533 27.106960963739823 28.020770072376905 23.269635857931743 94-95 21.271923797273047 26.955528567571513 27.993969885583915 23.778577749571532 96-97 21.23280111054689 26.719940386736635 28.144706199647114 23.902552303069363 98-99 21.111346597937175 26.792582223769053 27.820173571754438 24.275897606539335 100-101 21.213277879739266 26.177851181558687 28.52240530870619 24.086465629995853 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3229.0 1 2096.5 2 1232.0 3 1566.0 4 1346.0 5 1244.0 6 2205.0 7 4471.0 8 6756.0 9 7281.5 10 5966.0 11 4642.5 12 4550.5 13 4804.0 14 5076.5 15 5349.0 16 5551.0 17 5880.5 18 6234.0 19 6651.0 20 7446.0 21 8562.5 22 9851.5 23 11641.0 24 13757.5 25 16702.0 26 20981.0 27 25877.0 28 30111.5 29 34689.0 30 40658.0 31 47276.0 32 55603.0 33 63096.5 34 69733.0 35 75994.5 36 83251.5 37 90894.0 38 94072.0 39 94938.0 40 96221.0 41 99890.0 42 104256.0 43 108813.5 44 113200.5 45 117118.0 46 117981.5 47 124103.5 48 149256.5 49 150822.5 50 130491.5 51 121650.5 52 114368.0 53 112331.5 54 109986.5 55 101047.0 56 90678.5 57 80777.0 58 72101.0 59 63006.5 60 53064.5 61 43242.0 62 35173.0 63 27920.0 64 21053.5 65 16435.5 66 12207.0 67 9565.5 68 6994.0 69 4371.5 70 3102.0 71 2357.5 72 1922.5 73 1742.0 74 1472.0 75 1249.5 76 908.0 77 510.0 78 313.0 79 212.5 80 121.5 81 82.0 82 61.0 83 45.0 84 26.0 85 13.5 86 9.5 87 3.5 88 1.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23783516209593597 2 0.0 3 0.0 4 0.0 5 0.02921865667078981 6 0.0030570827905051713 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.003365730572238866 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.4092540247670734E-4 26-27 0.0013374737208460125 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006996016385963757 36-37 0.01791626885397021 38-39 0.014771000982969696 40-41 0.009024273237356611 42-43 0.008803810536118258 44-45 0.004115303756449269 46-47 6.025980500515E-4 48-49 0.0015726339355002562 50-51 5.8790053663560986E-5 52-53 0.0 54-55 0.0 56-57 0.0015285413952525857 58-59 3.086477817336952E-4 60-61 0.002792527549019147 62-63 0.001146406046439439 64-65 0.0015579364220843662 66-67 0.005776122772444867 68-69 3.086477817336952E-4 70-71 4.409254024767074E-5 72-73 0.0 74-75 5.438079963879392E-4 76-77 0.061038773216192194 78-79 0.14335954585859345 80-81 0.001146406046439439 82-83 0.0010435234525282074 84-85 0.0 86-87 0.10868811171050836 88-89 0.3604271214978765 90-91 0.39743546027908816 92-93 6.466905902991708E-4 94-95 3.086477817336952E-4 96-97 2.4985772807013416E-4 98-99 0.0 100-101 0.0015432389086684757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3401936.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.18841953374961 #Duplication Level Percentage of deduplicated Percentage of total 1 70.12599082330375 30.987567027200217 2 15.519674295960046 13.715797576340652 3 5.907494115789321 7.831284851449672 4 2.784307765890941 4.92136638681064 5 1.5381746324217787 3.3984752986812325 6 0.9288836279840281 2.462753967083378 7 0.6169804610678098 1.9084374020453456 8 0.42066152675106694 1.4870694420627026 9 0.314130775757381 1.2492848256866438 >10 1.5791801881800842 12.902632720860488 >50 0.15175680824186938 4.6726918879449775 >100 0.10225568683530631 8.38314004244967 >500 0.006917508538775794 2.0526581449726673 >1k 0.003325725257345232 2.524424458666659 >5k 6.651450514690463E-5 0.2707270277774639 >10k+ 1.995435154407139E-4 1.231688939967691 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17319 0.5090924696996063 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14579 0.4285500961805278 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10008 0.29418542853245916 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9211 0.27075759214753015 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4411 0.12966146335498377 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4145 0.12184238621773015 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3671 0.10790914349946619 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3513 0.10326472926004487 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.8790053663560986E-5 0.0 4 0.0 0.0 0.0 5.8790053663560986E-5 0.0 5 0.0 0.0 0.0 5.8790053663560986E-5 0.0 6 0.0 0.0 0.0 5.8790053663560986E-5 0.0 7 0.0 0.0 0.0 5.8790053663560986E-5 0.0 8 0.0 0.0 0.0 5.8790053663560986E-5 0.0 9 0.0 0.0 0.0 1.4697513415890245E-4 0.0 10-11 0.0 0.0 0.0 2.3516021465424392E-4 0.0 12-13 0.0 0.0 0.0 3.5274032198136593E-4 0.0 14-15 0.0 0.0 0.0 3.5274032198136593E-4 0.0 16-17 0.0 0.0 0.0 3.9683286222903666E-4 0.0 18-19 0.0 0.0 0.0 4.5562291589259764E-4 0.0 20-21 0.0 0.0 0.0 5.438079963879392E-4 0.0 22-23 0.0 0.0 0.0 8.083632378739636E-4 0.0 24-25 2.9395026831780493E-5 0.0 0.0 0.0014991463684208052 0.0 26-27 2.9395026831780493E-5 0.0 0.0 0.0022046270123835367 0.0 28-29 2.9395026831780493E-5 0.0 0.0 0.004791389373580221 0.0 30-31 2.9395026831780493E-5 0.0 0.0 0.014050822825591076 0.0 32-33 2.9395026831780493E-5 0.0 0.0 0.03292243005159415 0.0 34-35 2.9395026831780493E-5 0.0 0.0 0.061861833967482044 0.0 36-37 2.9395026831780493E-5 0.0 0.0 0.1093788948410552 0.0 38-39 2.9395026831780493E-5 0.0 0.0 0.1794713363214358 0.0 40-41 2.9395026831780493E-5 0.0 0.0 0.25794136044887384 0.0 42-43 2.9395026831780493E-5 0.0 0.0 0.33598515668725104 0.0 44-45 2.9395026831780493E-5 0.0 0.0 0.4333855780943557 0.0 46-47 2.9395026831780493E-5 0.0 0.0 0.5546547612888661 0.0 48-49 2.9395026831780493E-5 0.0 0.0 0.6847718475597424 0.0 50-51 2.9395026831780493E-5 0.0 0.0 0.8258385813254571 0.0 52-53 2.9395026831780493E-5 0.0 0.0 0.9647594781324516 0.0 54-55 2.9395026831780493E-5 0.0 0.0 1.116187958856369 0.0 56-57 2.9395026831780493E-5 0.0 0.0 1.3006123571989596 0.0 58-59 2.9395026831780493E-5 0.0 0.0 1.5086262645740542 0.0 60-61 2.9395026831780493E-5 0.0 0.0 1.738480676885162 0.0 62-63 2.9395026831780493E-5 0.0 0.0 1.9688054096255778 0.0 64-65 2.9395026831780493E-5 0.0 0.0 2.202319502777242 0.0 66-67 2.9395026831780493E-5 0.0 0.0 2.4734739277870013 0.0 68-69 2.9395026831780493E-5 0.0 0.0 2.7809018159071774 0.0 70-71 2.9395026831780493E-5 0.0 0.0 3.1228541630412803 0.0 72-73 2.9395026831780493E-5 0.0 0.0 3.464042239477756 0.0 74-75 2.9395026831780493E-5 0.0 0.0 3.808757719134046 0.0 76-77 2.9395026831780493E-5 0.0 0.0 4.176048579397143 0.0 78-79 2.9395026831780493E-5 0.0 0.0 4.6049808109264845 0.0 80-81 2.9395026831780493E-5 0.0 0.0 5.0725528052262 0.0 82-83 2.9395026831780493E-5 0.0 0.0 5.541976686216319 0.0 84-85 2.9395026831780493E-5 0.0 0.0 6.016926832250812 0.0 86-87 2.9395026831780493E-5 0.0 0.0 6.508044243042785 0.0 88-89 2.9395026831780493E-5 0.0 0.0 7.037448676283152 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18980 0.0 24.43863 1 TACATGG 19310 0.0 23.518589 2 ACATGGG 19305 0.0 22.860975 3 GAGTACT 15535 0.0 21.40976 12-13 GTATCAA 31780 0.0 20.852861 1 CATGGGG 7300 0.0 20.47714 4 GTACTTT 16270 0.0 20.47173 14-15 AGTACTT 15860 0.0 20.193222 12-13 TACCTGG 2755 0.0 19.981035 2 GGTATCA 24910 0.0 19.690346 1 GTATAGG 1040 0.0 19.212523 1 CATGGGA 11400 0.0 19.065237 4 ATCAACG 36855 0.0 17.897814 3 TCAACGC 36905 0.0 17.83499 4 GTGTAGC 1690 0.0 17.734636 1 CAACGCA 37250 0.0 17.708025 5 TATCAAC 37335 0.0 17.69313 2 CATTCCG 510 0.0 17.674103 9 ACTTTTT 19050 0.0 17.657562 16-17 AACGCAG 37975 0.0 17.378105 6 >>END_MODULE