##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938707_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1191383 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6644177397193 33.0 31.0 34.0 30.0 34.0 2 31.566680068458254 33.0 31.0 34.0 30.0 34.0 3 31.568403275856713 33.0 31.0 34.0 28.0 34.0 4 35.11218894343801 37.0 35.0 37.0 32.0 37.0 5 35.211711934785036 37.0 35.0 37.0 33.0 37.0 6 35.37490966381088 37.0 35.0 37.0 33.0 37.0 7 35.33508703750179 37.0 35.0 37.0 33.0 37.0 8 35.335573027313636 37.0 35.0 37.0 33.0 37.0 9 36.81734673064833 39.0 37.0 39.0 33.0 39.0 10-11 36.71599603150288 39.0 37.0 39.0 32.0 39.0 12-13 36.59414730611399 39.0 37.0 39.0 32.0 39.0 14-15 37.479117126902096 40.0 37.0 41.0 32.0 41.0 16-17 37.48274736167966 40.0 37.0 41.0 32.0 41.0 18-19 37.62495142200283 40.0 37.0 41.0 32.0 41.0 20-21 37.528447610885834 40.0 37.0 41.0 32.0 41.0 22-23 37.39889523352272 40.0 36.0 41.0 32.0 41.0 24-25 37.349553837850635 40.0 36.0 41.0 32.0 41.0 26-27 37.078095373192326 39.5 35.5 41.0 31.5 41.0 28-29 36.878950765622804 39.0 35.0 41.0 31.0 41.0 30-31 36.60293709075923 39.0 35.0 41.0 31.0 41.0 32-33 36.2525959326262 38.0 35.0 41.0 30.0 41.0 34-35 35.853153855645076 38.0 35.0 40.0 30.0 41.0 36-37 35.43629798310031 38.0 35.0 40.0 28.5 41.0 38-39 35.111486398580475 38.0 35.0 40.0 26.5 41.0 40-41 34.8565704731392 38.0 35.0 40.0 25.5 41.0 42-43 34.61291960687705 38.0 35.0 40.0 24.5 41.0 44-45 34.38180375244569 38.0 34.5 40.0 23.5 41.0 46-47 33.92579506338432 38.0 33.5 40.0 21.0 41.0 48-49 33.55894955694349 38.0 33.0 40.0 16.5 41.0 50-51 32.33372937166302 36.5 31.5 39.0 13.0 40.0 52-53 32.36650808346266 36.5 32.0 39.5 10.0 40.0 54-55 32.88761716425364 37.0 33.0 40.0 8.0 41.0 56-57 32.87979432306823 37.0 33.0 40.0 7.5 41.0 58-59 32.72297027907902 37.0 33.0 40.0 4.0 41.0 60-61 32.35104622107248 36.0 32.5 40.0 2.0 41.0 62-63 31.89350527915876 35.5 32.0 39.0 2.0 41.0 64-65 31.394619530411298 35.0 31.0 39.0 2.0 41.0 66-67 30.9500538449852 35.0 31.0 39.0 2.0 40.0 68-69 30.64167274503665 35.0 31.0 38.0 2.0 40.0 70-71 30.145032705687424 35.0 29.5 37.0 2.0 39.0 72-73 29.613996086900684 35.0 29.0 36.5 2.0 39.0 74-75 29.19882858828773 35.0 29.0 36.0 2.0 39.0 76-77 28.684168734991182 34.0 28.5 35.5 2.0 37.5 78-79 28.258272108969155 34.0 27.5 35.0 2.0 37.0 80-81 27.87621025312599 34.0 27.0 35.0 2.0 36.5 82-83 27.432963622949128 34.0 26.0 35.0 2.0 36.0 84-85 27.03832101012017 34.0 25.5 35.0 2.0 36.0 86-87 26.59166279861304 33.5 25.0 35.0 2.0 35.0 88-89 26.094553136984494 33.0 23.0 35.0 2.0 35.0 90-91 25.544155825624507 33.0 20.0 35.0 2.0 35.0 92-93 25.165752323140417 33.0 18.0 35.0 2.0 35.0 94-95 24.73595350949275 33.0 6.5 35.0 2.0 35.0 96-97 24.033579042171997 32.0 2.0 35.0 2.0 35.0 98-99 23.330738729694815 32.0 2.0 35.0 2.0 35.0 100-101 21.32664474816243 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3049010861371535 1101 2 -0.05451092840438676 1101 3 0.28218978343520007 1101 4 0.06847722991359717 1101 5 0.06197678660043948 1101 6 -0.043686625178573024 1101 7 0.030313024587997006 1101 8 0.26689289269484107 1101 9 0.2394566700429408 1101 10-11 0.4131500651246327 1101 12-13 0.31151601197261414 1101 14-15 0.625530066168352 1101 16-17 0.4934402586361273 1101 18-19 0.5535359122913945 1101 20-21 0.5517876351830822 1101 22-23 0.6553656292507597 1101 24-25 0.7257113438678431 1101 26-27 0.5646744052013446 1101 28-29 0.5292058442847605 1101 30-31 0.460389313268152 1101 32-33 0.6233740139855541 1101 34-35 0.521759259519456 1101 36-37 0.7994827720234596 1101 38-39 0.5396604484318956 1101 40-41 0.4160831221869117 1101 42-43 0.40376365842584505 1101 44-45 0.5316822929005625 1101 46-47 0.6435465644870462 1101 48-49 0.6132021355956354 1101 50-51 0.5864646829323803 1101 52-53 0.6839714479269361 1101 54-55 0.732294983813027 1101 56-57 0.8038522482869865 1101 58-59 0.7241104389934137 1101 60-61 0.37629112726884983 1101 62-63 0.4760888316545433 1101 64-65 0.5319831252485479 1101 66-67 0.45088433485172885 1101 68-69 0.5787943666926658 1101 70-71 0.5144716575027424 1101 72-73 0.462054208450148 1101 74-75 0.1978290779517735 1101 76-77 0.059942805305844615 1101 78-79 0.18928415341177285 1101 80-81 0.24308101313486574 1101 82-83 0.21997008411830166 1101 84-85 0.28602028387344447 1101 86-87 0.3826073810746884 1101 88-89 0.2517425466783756 1101 90-91 0.3002034088041974 1101 92-93 0.4162498193753983 1101 94-95 0.19960638734639602 1101 96-97 0.30543975717636584 1101 98-99 0.4040193703522945 1101 100-101 0.4644486735143616 1102 1 0.2243253571376087 1102 2 0.2879213765935944 1102 3 0.2320477188447967 1102 4 0.10645501395092793 1102 5 0.15005989438581935 1102 6 0.1828558778525462 1102 7 0.13697015904723742 1102 8 0.03771882683075489 1102 9 0.20984355111283293 1102 10-11 0.28778801474069127 1102 12-13 0.11790325432745874 1102 14-15 0.15031252976429954 1102 16-17 0.24830535850998103 1102 18-19 0.3512056243656545 1102 20-21 0.3838643806792632 1102 22-23 0.32324601943850695 1102 24-25 0.2565851329509101 1102 26-27 0.34525962558515744 1102 28-29 0.3737025253620061 1102 30-31 0.3397446339544743 1102 32-33 0.3001342065075647 1102 34-35 0.2672623335032611 1102 36-37 0.4768974107997508 1102 38-39 0.20028681855914243 1102 40-41 0.18109711432438047 1102 42-43 0.25196197936934794 1102 44-45 0.4711777799004224 1102 46-47 0.6594453985776525 1102 48-49 0.768081625632739 1102 50-51 0.6931685088787631 1102 52-53 0.6989708968142949 1102 54-55 0.5661812157946997 1102 56-57 0.6168107433820893 1102 58-59 0.6723617279846295 1102 60-61 0.6888646089535442 1102 62-63 0.6269245327310529 1102 64-65 0.8308668159886317 1102 66-67 0.8491213867665373 1102 68-69 0.5498532990037859 1102 70-71 0.615433961398498 1102 72-73 0.5528285829373054 1102 74-75 0.41480901132838355 1102 76-77 0.37559103623428314 1102 78-79 0.31262851920359935 1102 80-81 0.2695497283076058 1102 82-83 0.32309856684398497 1102 84-85 0.29795952676715487 1102 86-87 0.1216111531345021 1102 88-89 0.22169772285071687 1102 90-91 0.07621669061870762 1102 92-93 -0.09891879047359708 1102 94-95 0.02220093378069876 1102 96-97 -0.020574749332883613 1102 98-99 -0.18138012507347412 1102 100-101 -0.20140408143646837 1103 1 0.10297800921407685 1103 2 0.2584261345326766 1103 3 0.08725971350513007 1103 4 0.2529177893541572 1103 5 0.1738648984885529 1103 6 0.331313374821427 1103 7 0.10461372388869705 1103 8 0.08070408150602759 1103 9 0.40466646025272723 1103 10-11 0.36813258260715287 1103 12-13 0.39412090707750735 1103 14-15 0.37946363260191873 1103 16-17 0.31424445444032756 1103 18-19 0.4656862619417481 1103 20-21 0.5115778449732886 1103 22-23 0.45082017470530644 1103 24-25 0.5631239312804297 1103 26-27 0.5978911884181244 1103 28-29 0.5877722778512009 1103 30-31 0.44203266991150514 1103 32-33 0.448111776223314 1103 34-35 0.3832103084705025 1103 36-37 0.6447624923031867 1103 38-39 0.4548702386416821 1103 40-41 0.4466775277813184 1103 42-43 0.4579594626216519 1103 44-45 0.755895579613842 1103 46-47 0.6160115994520794 1103 48-49 0.7093559817494821 1103 50-51 0.6817444032121003 1103 52-53 0.8491812381367225 1103 54-55 0.9783614173794533 1103 56-57 0.859359241293987 1103 58-59 0.8775195299025071 1103 60-61 0.8505044139821365 1103 62-63 0.5731168036825167 1103 64-65 0.5695705378359612 1103 66-67 0.5339262928936854 1103 68-69 0.5267838771821758 1103 70-71 0.6346639651950525 1103 72-73 0.6137150476109845 1103 74-75 0.686028378651077 1103 76-77 0.6875651829282248 1103 78-79 0.6232876499152695 1103 80-81 0.6543572369110855 1103 82-83 0.6574700841183017 1103 84-85 0.6719468573000178 1103 86-87 0.34545703142433837 1103 88-89 0.496060728496559 1103 90-91 0.35658452768531745 1103 92-93 0.3996414277670084 1103 94-95 0.44305044329045273 1103 96-97 0.7057894075260158 1103 98-99 0.8187920976250211 1103 100-101 1.0300081140738016 1104 1 0.04474955050231344 1104 2 0.18377824288127087 1104 3 0.09432799671576575 1104 4 0.019366411031249697 1104 5 0.08110923587348395 1104 6 0.050456270589975816 1104 7 -0.015023229667413318 1104 8 0.05234952744848442 1104 9 0.17754582166460153 1104 10-11 0.09373847934661939 1104 12-13 0.1662459700637271 1104 14-15 0.1443391349554517 1104 16-17 0.20492341519837254 1104 18-19 0.19133594178737923 1104 20-21 0.32348792522694936 1104 22-23 0.5206188953168507 1104 24-25 0.3590800621442938 1104 26-27 0.3083343041775848 1104 28-29 0.24690850629761485 1104 30-31 0.28039926816386895 1104 32-33 0.1555184951634203 1104 34-35 0.17210506924575242 1104 36-37 0.3617170008877437 1104 38-39 0.3457289405724353 1104 40-41 0.24291786477297705 1104 42-43 0.20246967560725437 1104 44-45 0.2932917291585042 1104 46-47 0.12858119154715553 1104 48-49 0.21172334198151788 1104 50-51 0.2842391185143711 1104 52-53 0.22956732832070514 1104 54-55 0.28756232192306896 1104 56-57 0.20033362280000233 1104 58-59 0.0867720524239175 1104 60-61 0.1991177953283838 1104 62-63 0.20259420238222958 1104 64-65 0.14487901670331382 1104 66-67 0.18450484439491177 1104 68-69 0.26978549331647983 1104 70-71 0.10357258293934635 1104 72-73 0.18832222865489712 1104 74-75 -0.01845101717278297 1104 76-77 0.05415268154559527 1104 78-79 0.03821016149858991 1104 80-81 -0.015107195844205279 1104 82-83 0.15258711559297566 1104 84-85 0.298677442180864 1104 86-87 0.27085599537778293 1104 88-89 0.13976225982990087 1104 90-91 0.026584343335397165 1104 92-93 -0.04901174243713058 1104 94-95 -0.006712667061410826 1104 96-97 0.16578624285237709 1104 98-99 0.22952141663641612 1104 100-101 0.3443155114216232 1105 1 0.16137545009654986 1105 2 0.16771220774090523 1105 3 0.04547290749676591 1105 4 0.1808528782173866 1105 5 0.13093627023057763 1105 6 0.18822592335074262 1105 7 0.20051781979279326 1105 8 0.16762838474769381 1105 9 0.3093687938472769 1105 10-11 0.35822557344864236 1105 12-13 0.3276170516515009 1105 14-15 0.32033787811688086 1105 16-17 0.11003623750755764 1105 18-19 0.09247077981908092 1105 20-21 0.036906932053874186 1105 22-23 -0.06597104305897261 1105 24-25 -0.014428381676076185 1105 26-27 0.05068465243913067 1105 28-29 0.200343464149924 1105 30-31 0.26295800206438713 1105 32-33 0.26772666732023964 1105 34-35 0.12243814650073404 1105 36-37 0.04833309834384636 1105 38-39 0.09189189961544031 1105 40-41 -0.02923316332397974 1105 42-43 0.06562761781974302 1105 44-45 0.08617495592615398 1105 46-47 -0.08139428807356808 1105 48-49 -0.047751431795056476 1105 50-51 0.22102660304819466 1105 52-53 0.1718952008207424 1105 54-55 0.25305977291613146 1105 56-57 0.10626241297260464 1105 58-59 0.038236058882510804 1105 60-61 0.19388184493441685 1105 62-63 0.22593516795297575 1105 64-65 0.10570880077063194 1105 66-67 -0.028870939813725016 1105 68-69 0.06415257136224639 1105 70-71 -0.051599247640019996 1105 72-73 -0.08033603603590578 1105 74-75 -0.13013375263081528 1105 76-77 -0.1905948307108325 1105 78-79 -0.18987822612485772 1105 80-81 -0.2867230151721607 1105 82-83 -0.3346997968988852 1105 84-85 -0.3854362536010605 1105 86-87 -0.40120685857787564 1105 88-89 -0.5682832359222552 1105 90-91 -0.3686071967579032 1105 92-93 -0.4654594145044939 1105 94-95 -0.3066272742016345 1105 96-97 -0.40298019113879135 1105 98-99 -0.396170709029402 1105 100-101 -0.3340559655591413 1106 1 0.13588402613935813 1106 2 0.018219099886064072 1106 3 0.17250306035092677 1106 4 0.23430089836423207 1106 5 0.2516360765224235 1106 6 0.06814484457758851 1106 7 -0.0968301912154601 1106 8 0.15505683033338613 1106 9 0.05923059139303888 1106 10-11 0.12754283085278217 1106 12-13 0.10039011947631593 1106 14-15 0.03472583974679111 1106 16-17 0.10907593644343194 1106 18-19 0.300417533297356 1106 20-21 0.21223565913810916 1106 22-23 0.09645092311625802 1106 24-25 0.1335553606216635 1106 26-27 0.08737871490215099 1106 28-29 0.14077783184185932 1106 30-31 0.06259927493520223 1106 32-33 0.23664909267218803 1106 34-35 0.29134289392357715 1106 36-37 0.25054014284878434 1106 38-39 0.314997851834093 1106 40-41 0.389941247424197 1106 42-43 0.4603682840978962 1106 44-45 0.36522331405104325 1106 46-47 0.4431581831660907 1106 48-49 0.6813916081196041 1106 50-51 0.5697009081782411 1106 52-53 0.4734892856058863 1106 54-55 0.3533966273533693 1106 56-57 0.4723273808601931 1106 58-59 0.31115332167990317 1106 60-61 0.5032253795314787 1106 62-63 0.23463031206555485 1106 64-65 -0.02027153777669355 1106 66-67 0.10131052862292833 1106 68-69 0.34582527798802687 1106 70-71 0.22182625561916325 1106 72-73 0.2476622081562212 1106 74-75 0.2821470148310077 1106 76-77 0.3392512814181714 1106 78-79 0.4628036505521109 1106 80-81 0.48539911534788516 1106 82-83 0.4014698453028238 1106 84-85 0.3093430644203927 1106 86-87 0.56556912161086 1106 88-89 0.4852704023603458 1106 90-91 0.5840976013017176 1106 92-93 0.7767379520833195 1106 94-95 0.7940923523446237 1106 96-97 0.6917720107371643 1106 98-99 0.7542591043485949 1106 100-101 1.0761791059540755 1107 1 0.10541396536540049 1107 2 -0.007370516339541666 1107 3 0.03211638915525583 1107 4 0.18542954711779913 1107 5 0.2224416366118902 1107 6 0.05142575614287637 1107 7 -0.1685597860606265 1107 8 -0.036344382204902104 1107 9 0.04443338937733898 1107 10-11 -0.0972686198847228 1107 12-13 0.08774235699618771 1107 14-15 0.10530590535854856 1107 16-17 0.17873439355243192 1107 18-19 0.18047792493869252 1107 20-21 0.1588878712190862 1107 22-23 0.10107844917641984 1107 24-25 0.03170640883646314 1107 26-27 -0.060065779047654644 1107 28-29 -0.1571983791156839 1107 30-31 -0.15065916805740898 1107 32-33 -0.0392735819114165 1107 34-35 -0.01712906667994929 1107 36-37 0.20857037837177472 1107 38-39 0.1947990755478486 1107 40-41 0.114084375761216 1107 42-43 0.09868528801371923 1107 44-45 0.13433520254339015 1107 46-47 0.5300478151255774 1107 48-49 0.5386841892263021 1107 50-51 0.7551375822413036 1107 52-53 0.7414523800930013 1107 54-55 0.43632117382787783 1107 56-57 0.3056430687176146 1107 58-59 0.3519695131686049 1107 60-61 0.1275215823721041 1107 62-63 -0.014866424256631205 1107 64-65 0.10386254725391453 1107 66-67 0.12008016840524505 1107 68-69 0.27479892888110413 1107 70-71 0.39732190968548764 1107 72-73 0.12211979357870462 1107 74-75 0.17886142721460274 1107 76-77 -0.011049279855008365 1107 78-79 0.03630955663994584 1107 80-81 -0.10691135386002415 1107 82-83 -0.22201907701443702 1107 84-85 -0.019112765277238708 1107 86-87 0.327070663384319 1107 88-89 0.37375172977068516 1107 90-91 0.23083443814429572 1107 92-93 0.15750085423474758 1107 94-95 0.32536638511520977 1107 96-97 0.27374480435421233 1107 98-99 0.3199440208487303 1107 100-101 0.13866394187320807 1108 1 0.08644499827808261 1108 2 0.09160145837964251 1108 3 -0.05518221438455839 1108 4 -0.11849583019379395 1108 5 0.019424397033120044 1108 6 0.04001346411853035 1108 7 -0.02016448297906237 1108 8 0.038219824469528874 1108 9 0.023340282284756597 1108 10-11 0.018512675151427516 1108 12-13 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-3.4126211086152054 2316 100-101 -3.4075539596863784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4171.0 3 1224.0 4 1192.0 5 1304.0 6 1517.0 7 1985.0 8 2399.0 9 3073.0 10 3980.0 11 5656.0 12 7941.0 13 10829.0 14 11359.0 15 12140.0 16 15360.0 17 19806.0 18 16045.0 19 10750.0 20 10329.0 21 10716.0 22 11656.0 23 12702.0 24 14311.0 25 16023.0 26 18048.0 27 20990.0 28 24227.0 29 28465.0 30 33023.0 31 39732.0 32 48380.0 33 61184.0 34 84643.0 35 146851.0 36 147895.0 37 176730.0 38 133531.0 39 21190.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.40134996422906 16.984766034503195 12.773042413155636 16.84084158811211 2 13.274579403919262 24.80015122555712 40.19104828716053 21.73422108336309 3 24.09908793600683 32.66805390225114 28.54809862757765 14.684759534164387 4 10.56860087701978 16.42349350973435 39.78643333246511 33.22147228078076 5 10.703240553986824 43.4533414011026 31.62943727309399 14.213980771816592 6 23.401316446853013 36.32865996659761 21.309007307715884 18.961016278833487 7 24.3885194142147 39.16003291683461 20.760362085180706 15.691085583769985 8 23.25556789039053 30.204493276208677 21.697431909751554 24.84250692364924 9 28.051997128065604 20.71420473869176 22.821969458405235 28.411828674837402 10-11 22.66721528633538 27.08609480063903 26.742143857314097 23.504546055711486 12-13 31.681464680012734 25.17578326739799 26.036875647141912 17.105876405447372 14-15 26.793989572823335 25.398366938031902 31.358577060295318 16.449066428849445 16-17 17.685336222044477 29.808140035311137 33.135238287179035 19.371285455465355 18-19 16.68431580679944 31.594788059160027 35.32050614542779 16.400389988612734 20-21 19.41746268286958 28.717594068057444 32.304296325035786 19.560646924037194 22-23 17.878140638097364 28.2884204797237 31.005491339675224 22.827947542503708 24-25 19.28190584517318 28.5352582869134 33.169494985303274 19.013340882610148 26-27 21.762929883012344 25.18531866241257 35.492304151104435 17.55944730347065 28-29 17.884785723015515 27.17938250496534 37.29111254187829 17.644719230140858 30-31 17.39330725260401 30.97566731784503 34.63921751093089 16.99180791862006 32-33 16.97968068203576 30.480950429463515 33.32662908930893 19.212739799191798 34-35 18.810191265110955 27.305830555274945 33.876401656733 20.0075765228811 36-37 20.793461513486072 27.14772314233934 35.2165538435071 16.842261500667494 38-39 22.139655002331292 25.49629564676313 35.75571419778641 16.60833515311917 40-41 18.064783423050404 28.24089918251537 37.08837061101455 16.60594678341968 42-43 16.694871451108757 31.189137321464557 33.83579166065892 18.280199566767767 44-45 17.439127740042245 31.171361472655445 31.482142028948847 19.907368758353467 46-47 17.791420613565013 29.86320810801011 30.110912572761865 22.23445870566301 48-49 19.478827196728943 29.32008177641034 30.92035571389052 20.280735312970197 50-51 20.784249402728435 30.376940974473964 31.829788777113343 17.009020845684255 52-53 17.983681789411676 31.01016630676195 32.84802069272908 18.158131211097295 54-55 15.77423681446541 30.0169374251319 34.108735992471125 20.100089767931557 56-57 18.912889518413596 31.683613246998515 29.97571833265884 19.427778901929045 58-59 18.784471240775005 29.66539241263914 30.822041363381565 20.728094983204294 60-61 18.50141843463635 28.167461661395027 33.014829836297515 20.31629006767111 62-63 22.22538874847968 27.58403649187301 31.613614470275525 18.576960289371783 64-65 20.27202616093581 29.42206987355182 33.027717551975414 17.278186413536954 66-67 17.8797724604235 29.465346100381932 33.378375642738575 19.276505796455996 68-69 17.21627712463953 32.41573419951655 31.7975199727019 18.570468703142023 70-71 18.00996082480488 31.880797405866495 29.407757768397325 20.7014840009313 72-73 18.128077114157193 29.906576347013708 29.27982981982461 22.685516719004493 74-75 18.275413607902408 31.37119791104394 30.38672844920652 19.966660031847134 76-77 20.234086779072168 28.395206955143205 31.772768860386968 19.597937405397655 78-79 18.40535303373846 28.435420431414254 33.84059684606361 19.318629688783677 80-81 17.874146462376896 32.158523825703384 31.618009717619106 18.349319994300618 82-83 17.516069111302045 31.225594892286573 30.296946552656006 20.961389443755372 84-85 18.32388555413059 29.191023893058393 30.45498582200738 22.03010473080364 86-87 19.91231654082935 29.424976799469416 31.204239298768442 19.458467360932794 88-89 20.98041076048249 27.264653978253854 31.741441435743035 20.01349382552062 90-91 18.61938122349355 28.394712394633114 33.24796730310957 19.737939078763763 92-93 17.75709447534851 31.533236593842158 31.62487343531042 19.084795495498916 94-95 17.497053186038322 32.02122971231927 30.149143578897647 20.332573522744767 96-97 17.288342885579695 29.990257973911618 29.36335226913296 23.358046871375734 98-99 19.463363762146678 29.62935942617783 29.089239906600817 21.81803690507467 100-101 21.16881298874119 29.20704284857741 30.17012599645648 19.45401816622492 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15732.0 1 9976.0 2 9704.0 3 12360.5 4 7150.5 5 5443.5 6 8747.5 7 15118.5 8 19994.5 9 18720.0 10 12560.0 11 7880.0 12 6900.5 13 6737.5 14 6413.5 15 5799.0 16 5332.0 17 5079.5 18 4799.0 19 4666.0 20 4770.0 21 5059.0 22 5377.0 23 5922.5 24 6333.0 25 6876.0 26 7920.5 27 8982.5 28 9819.5 29 10724.5 30 11909.5 31 13059.5 32 14543.0 33 16210.0 34 17711.5 35 18885.5 36 20216.0 37 21815.0 38 22395.0 39 22043.0 40 21466.0 41 21432.0 42 21694.5 43 22178.5 44 25400.5 45 28125.5 46 34144.5 47 54894.5 48 134944.5 49 134454.0 50 60514.5 51 40420.5 52 23540.0 53 17290.5 54 15007.0 55 13511.0 56 12514.5 57 11453.0 58 10657.0 59 10031.0 60 9668.5 61 9526.5 62 8203.0 63 5292.0 64 2982.0 65 2162.0 66 1567.5 67 1136.5 68 901.0 69 732.5 70 612.5 71 500.5 72 448.5 73 509.0 74 458.5 75 329.0 76 249.0 77 171.0 78 113.0 79 80.0 80 68.0 81 55.5 82 41.5 83 25.0 84 14.5 85 8.0 86 5.0 87 4.0 88 4.0 89 4.0 90 2.5 91 1.5 92 3.0 93 3.0 94 2.5 95 1.5 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1569604400935719 2 0.09300115915704689 3 0.08443968060648843 4 0.10206625409293234 5 0.1227984619555592 6 0.037771228899522655 7 0.042219840303244216 8 0.045073666486763704 9 0.045996963193196475 10-11 0.04326904110600873 12-13 0.04784355660606203 14-15 0.04637467548219171 16-17 0.04536744271153777 18-19 0.04859898118405249 20-21 0.051159031142797906 22-23 0.0655540661567271 24-25 0.08242521506518055 26-27 0.08402000028538262 28-29 0.0738217684825115 30-31 0.06051790230345741 32-33 0.049941958211591066 34-35 0.2940280329667286 36-37 0.4703777038953888 38-39 0.4487221993263292 40-41 0.4248004210232982 42-43 0.45442985169336814 44-45 0.46148048108794565 46-47 0.46114473683106105 48-49 0.4793588627670531 50-51 0.467859621968754 52-53 0.47444860301011516 54-55 0.46563531626689314 56-57 0.44511294856481914 58-59 0.42538797347284624 60-61 0.3934503010366943 62-63 0.4503589525786418 64-65 0.4867032683864047 66-67 0.48943119047359246 68-69 0.49962942227646356 70-71 0.49849628540947793 72-73 0.488172149510275 74-75 0.48003035128082233 76-77 0.46148048108794565 78-79 0.4376846068812464 80-81 0.44402177972994405 82-83 0.45426197956492576 84-85 0.4524153861520603 86-87 0.46387265891824875 88-89 0.4747004112027786 90-91 0.47881327834961557 92-93 0.4804919996340387 94-95 0.48519241923042383 96-97 0.48666130035429417 98-99 0.4800723193129329 100-101 0.4663487728127731 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1191383.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.29738451527216 #Duplication Level Percentage of deduplicated Percentage of total 1 78.15376972697477 29.14931200825015 2 12.041976153974682 8.982684298770637 3 4.14696368455892 4.6401269734159145 4 1.8608410462419116 2.7761801609394365 5 1.0073652496155503 1.8786044531117148 6 0.6028869036188147 1.3491662798095654 7 0.42183330825563936 1.1013295369551936 8 0.2847469290683721 0.8496252562404801 9 0.2136765614984289 0.717261918849729 >10 1.0715807446899195 7.378514588248122 >50 0.10482907982083466 2.71960183356399 >100 0.06253645741670603 4.726475890068699 >500 0.010347759135094014 2.757320687436613 >1k 0.011922418133912668 9.299748650098863 >5k 0.0022495128554552205 5.418910395553448 >10k+ 0.0024744641410007423 16.255137068687446 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 32010 2.6867934157193782 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 28721 2.410727700495978 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22511 1.8894847416825657 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 18767 1.575228117238537 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15890 1.3337440604742556 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15717 1.3192231213639947 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14456 1.21337974438111 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12805 1.0748013023519725 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11547 0.9692097335617513 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 11274 0.9462951880293743 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 10044 0.8430538290373457 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 9164 0.7691900925227235 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 7462 0.6263309112183069 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7336 0.6157549671264404 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7323 0.6146637982915654 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6088 0.511002758978431 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5965 0.5006786230792282 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5565 0.4671041973907635 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5481 0.46005356799618596 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5102 0.4282417996563657 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5101 0.4281578635921446 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4800 0.402893108261575 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4491 0.3769568644172361 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4356 0.36562549574737935 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4246 0.35639252868305155 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4126 0.34632020097651217 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4029 0.3381784027470595 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3884 0.3260076734349911 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3387 0.28429144951707386 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3362 0.2821930479115448 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3149 0.26431466623243743 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3087 0.2591106302507254 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2937 0.24652022061755122 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2587 0.2171425981401447 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2444 0.2051397409565186 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2264 0.19003124939670954 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2228 0.1870095510847477 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2182 0.1831484921305743 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2116 0.17760871189197763 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2091 0.17551031028644862 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2076 0.17425126932313117 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2020 0.16955084972674614 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1983 0.16644521535056317 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1916 0.16082149904774534 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1870 0.1569604400935719 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1830 0.15360299752472548 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1819 0.15267970081829269 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1796 0.15074917134120597 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1763 0.14797928122190765 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1650 0.13849450596491641 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1553 0.13035270773546376 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1517 0.12733100942350192 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1481 0.12430931111154012 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1426 0.11969282757937623 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1418 0.11902133906560694 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1404 0.11784623416651069 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1383 0.11608357681786628 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1347 0.11306187850590448 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1329 0.11155102934992357 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1326 0.1112992211572601 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1321 0.11087954083615428 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1309 0.10987230806550036 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1297 0.1088650752948464 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1295 0.10869720316640408 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1241 0.10416465569846137 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1193 0.10013572461584563 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.196803211058073E-5 0.0 14-15 0.0 4.196803211058073E-5 0.0 2.518081926634844E-4 0.0 16-17 0.0 8.393606422116146E-5 0.0 5.455844174375495E-4 0.0 18-19 0.0 8.393606422116146E-5 0.0 9.652647385433568E-4 0.0 20-21 0.0 8.393606422116146E-5 0.0 0.0017626573486443906 0.0 22-23 0.0 8.393606422116146E-5 0.0 0.003819090922062846 0.0 24-25 0.0 8.393606422116146E-5 0.0 0.007722117908346853 0.0 26-27 0.0 8.393606422116146E-5 0.0 0.011960889151515507 0.0 28-29 0.0 8.393606422116146E-5 0.0 0.02312438569292998 0.0 30-31 0.0 1.259040963317422E-4 0.0 0.04948030985837468 0.0 32-33 0.0 2.0984016055290364E-4 0.0 0.09644253779011452 0.0 34-35 0.0 2.5180819266348435E-4 0.0 0.17530047012589572 0.0 36-37 0.0 2.5180819266348435E-4 0.0 0.28794266831069437 0.0 38-39 0.0 2.5180819266348435E-4 0.0 0.41749798343605704 0.0 40-41 0.0 2.5180819266348435E-4 0.0 0.564050351566205 0.0 42-43 0.0 2.5180819266348435E-4 0.0 0.707077404999064 0.0 44-45 0.0 2.5180819266348435E-4 0.0 0.8599249779457991 0.0 46-47 0.0 2.5180819266348435E-4 0.0 1.053523510071908 0.0 48-49 0.0 2.5180819266348435E-4 0.0 1.2780105138314044 0.0 50-51 0.0 2.5180819266348435E-4 0.0 1.5030850700404488 0.0 52-53 0.0 2.5180819266348435E-4 0.0 1.7197240517952666 0.0 54-55 0.0 2.5180819266348435E-4 0.0 1.9448825440685322 0.0 56-57 0.0 2.5180819266348435E-4 0.0 2.2065951923101137 0.0 58-59 0.0 2.5180819266348435E-4 0.0 2.5684855331996514 0.0 60-61 0.0 2.5180819266348435E-4 0.0 3.050068701668565 0.0 62-63 0.0 2.5180819266348435E-4 0.0 3.460348183581602 0.0 64-65 0.0 2.5180819266348435E-4 0.0 3.7741011916403036 0.0 66-67 0.0 2.5180819266348435E-4 0.0 4.1298641998416965 0.0 68-69 0.0 2.5180819266348435E-4 0.0 4.533302892520709 0.0 70-71 0.0 2.5180819266348435E-4 0.0 4.996084382604083 0.0 72-73 0.0 2.5180819266348435E-4 0.0 5.487488070586872 0.0 74-75 0.0 2.5180819266348435E-4 0.0 5.956564765486833 0.0 76-77 0.0 2.5180819266348435E-4 0.0 6.3874085831340555 0.0 78-79 0.0 2.5180819266348435E-4 0.0 6.900257935525351 0.0 80-81 0.0 2.5180819266348435E-4 0.0 7.462923342031908 0.0 82-83 0.0 2.5180819266348435E-4 0.0 8.038766710621186 0.0 84-85 0.0 2.5180819266348435E-4 0.0 8.78923906082259 0.0 86-87 0.0 2.5180819266348435E-4 0.0 9.580294498074927 0.0 88-89 0.0 2.5180819266348435E-4 0.0 10.210402532183185 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 65 6.85759E-10 58.31885 2 TACCTGG 840 0.0 49.07636 2 ACCTGGG 1140 0.0 36.161522 3 AGTACTC 655 0.0 34.000782 5 TACACGG 115 1.0589247E-7 32.96283 2 GTAAGCG 60 1.490098E-7 31.707312 94-95 GTACGTG 75 3.112958E-4 31.606647 1 TCACGCA 105 1.9383606E-6 31.58938 4 ATCACGC 105 1.9383606E-6 31.58938 3 ATGGGCG 180 5.456968E-12 31.589378 5 TGGGAGT 735 0.0 28.987505 6 GAGCCGC 115 3.960913E-6 28.821882 9 CATGGGG 2715 0.0 28.796947 4 AAGTACT 805 0.0 27.665232 4 GGAACGC 335 0.0 26.853104 8 TACGTGG 90 9.0762967E-4 26.324482 2 ATGGGAG 2790 0.0 24.9658 5 CATGGGT 830 0.0 24.548372 4 CTTACAC 330 0.0 24.409973 3 AAAGTAC 895 0.0 24.353823 3 >>END_MODULE