##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938707_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1191383 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.937387892894225 31.0 31.0 34.0 30.0 34.0 2 32.0660526463782 33.0 31.0 34.0 30.0 34.0 3 32.14701401648336 34.0 31.0 34.0 30.0 34.0 4 35.751636543412154 37.0 35.0 37.0 35.0 37.0 5 35.7082374014066 37.0 35.0 37.0 33.0 37.0 6 35.81561093284024 37.0 35.0 37.0 35.0 37.0 7 35.768602540073175 37.0 35.0 37.0 33.0 37.0 8 35.79186290219015 37.0 35.0 37.0 33.0 37.0 9 37.36960994071596 39.0 37.0 39.0 34.0 39.0 10-11 37.28361408547881 39.0 37.0 39.0 33.5 39.0 12-13 37.211424873445395 39.0 37.0 39.0 33.0 39.0 14-15 38.15214041160567 40.0 37.0 41.0 33.0 41.0 16-17 38.13597726339893 40.0 37.5 41.0 33.0 41.0 18-19 38.3327745989325 40.0 37.5 41.0 33.5 41.0 20-21 38.405280669608345 40.0 38.0 41.0 34.0 41.0 22-23 38.31582622884496 40.0 37.5 41.0 34.0 41.0 24-25 38.17404310788386 40.0 37.0 41.0 33.0 41.0 26-27 37.80244094468362 40.0 36.5 41.0 33.0 41.0 28-29 37.541320045694796 39.5 36.0 41.0 33.0 41.0 30-31 37.24447511841281 39.0 35.5 41.0 32.5 41.0 32-33 36.872653042724295 39.0 35.0 41.0 32.0 41.0 34-35 36.669808953124225 39.0 35.0 41.0 31.0 41.0 36-37 36.39196463269998 39.0 35.0 41.0 30.5 41.0 38-39 36.14299977421199 38.0 35.0 41.0 30.0 41.0 40-41 35.88324031818483 38.0 35.0 40.5 30.0 41.0 42-43 35.711941499920684 38.0 35.0 40.5 30.0 41.0 44-45 35.37687670547591 38.0 35.0 41.0 28.0 41.0 46-47 34.85097529509822 38.0 35.0 40.0 24.5 41.0 48-49 34.43793053954941 38.0 34.0 40.0 23.0 41.0 50-51 34.176616167932565 38.0 34.0 40.0 22.5 41.0 52-53 33.832877840291495 38.0 33.5 40.0 21.0 41.0 54-55 33.648645733571826 37.5 33.0 40.0 20.0 41.0 56-57 33.51159912471472 37.0 33.0 40.0 20.0 41.0 58-59 33.31115644591202 37.0 33.0 40.0 18.5 41.0 60-61 32.88550113607463 36.5 33.0 40.0 15.0 41.0 62-63 32.40843624594274 36.0 32.0 39.0 12.0 41.0 64-65 31.947253318202456 35.0 31.0 39.0 8.0 40.0 66-67 31.53388666784737 35.0 31.0 38.5 6.0 40.0 68-69 31.192463716537837 35.0 31.0 37.5 2.0 40.0 70-71 30.67627370879054 35.0 30.5 37.0 2.0 39.0 72-73 30.284420291375653 35.0 30.0 36.5 2.0 39.0 74-75 29.892771258277143 35.0 30.0 36.0 2.0 38.5 76-77 27.775920505832296 32.0 26.5 34.5 2.0 36.0 78-79 28.789689377807136 34.0 29.0 35.0 2.0 37.0 80-81 28.910282419675283 34.0 29.0 35.0 2.0 36.5 82-83 28.6569948538799 34.0 29.0 35.0 2.0 36.0 84-85 28.323026684114176 34.0 29.0 35.0 2.0 36.0 86-87 27.73292551597597 34.0 27.0 35.0 2.0 35.0 88-89 27.08658844385055 33.0 25.5 35.0 2.0 35.0 90-91 26.557025322671215 33.0 25.0 35.0 2.0 35.0 92-93 26.37177129436965 33.0 25.0 35.0 2.0 35.0 94-95 26.094634554966788 33.0 24.0 35.0 2.0 35.0 96-97 25.49663836062794 33.0 21.5 35.0 2.0 35.0 98-99 24.764419586312712 32.5 10.0 35.0 2.0 35.0 100-101 21.81897592965486 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.01002739361877758 1101 2 0.038765113367162485 1101 3 0.0564221701212162 1101 4 0.06484538814319052 1101 5 -0.01905010204880142 1101 6 0.04897740902661951 1101 7 0.03826816613048578 1101 8 0.02864599469415907 1101 9 0.040275633491717144 1101 10-11 0.18645988755850595 1101 12-13 0.28483906939998604 1101 14-15 0.13407855897882115 1101 16-17 0.20933595070420097 1101 18-19 0.26066220777883586 1101 20-21 0.23942105406161573 1101 22-23 0.27514579658582505 1101 24-25 0.18852343117889347 1101 26-27 0.18680352058613892 1101 28-29 0.07629001773764799 1101 30-31 0.1411280238279531 1101 32-33 0.26201613099315324 1101 34-35 0.3597032325785676 1101 36-37 0.31373881426412 1101 38-39 0.5643590389552386 1101 40-41 0.47365527611600555 1101 42-43 0.5148058743629988 1101 44-45 0.6640755002324426 1101 46-47 0.7558480837797035 1101 48-49 0.6423937037006056 1101 50-51 0.7850455567689707 1101 52-53 0.7314001090088453 1101 54-55 0.7112928696670124 1101 56-57 0.5321297344424494 1101 58-59 0.6080491087588626 1101 60-61 0.7838879391372799 1101 62-63 0.868953374208786 1101 64-65 0.8756850128819238 1101 66-67 0.7293973921794503 1101 68-69 0.7435723141637887 1101 70-71 0.7101135743927642 1101 72-73 0.798541623778231 1101 74-75 0.7783795515795155 1101 76-77 0.7945780768769701 1101 78-79 0.9754616831595726 1101 80-81 1.0388276945746284 1101 82-83 0.9388897437143129 1101 84-85 0.8146434214840035 1101 86-87 1.0255591102826251 1101 88-89 1.0890753772763766 1101 90-91 1.2495099842559974 1101 92-93 1.159543217401474 1101 94-95 1.0130286702584677 1101 96-97 0.9350907329172955 1101 98-99 1.2199813061562566 1101 100-101 1.7306957674811727 1102 1 -0.05566870502191179 1102 2 0.0267263640254356 1102 3 0.011275329221312802 1102 4 0.12496259143026833 1102 5 0.03471552811787859 1102 6 -0.037058927666457464 1102 7 -0.046096462235894364 1102 8 0.07739956161760375 1102 9 0.09620744248832125 1102 10-11 0.18162999765856824 1102 12-13 0.1583388183375689 1102 14-15 0.1258662152804888 1102 16-17 0.171022141046258 1102 18-19 0.2162157391861328 1102 20-21 0.3268282834346934 1102 22-23 0.13961036004338467 1102 24-25 0.05389886135291988 1102 26-27 -0.013274094443318063 1102 28-29 0.06953276448302148 1102 30-31 0.04857324935549201 1102 32-33 -0.07177289084315674 1102 34-35 -0.07975551357895938 1102 36-37 0.047520794105643915 1102 38-39 0.014009541692423966 1102 40-41 0.08776773983441899 1102 42-43 0.1076675879558806 1102 44-45 0.06526712821916902 1102 46-47 -0.04822907811269772 1102 48-49 -0.023341174381030783 1102 50-51 -0.05208764715495562 1102 52-53 0.05164859957257306 1102 54-55 0.150694208258372 1102 56-57 0.10230933592678326 1102 58-59 0.01514988888941815 1102 60-61 -0.06559095324879394 1102 62-63 -0.3568359415542588 1102 64-65 -0.14685166672568783 1102 66-67 -0.0012516654179393072 1102 68-69 0.10694077582472161 1102 70-71 -0.006726284522400761 1102 72-73 -0.102317805758954 1102 74-75 -0.03433783173769456 1102 76-77 -0.11896995565092183 1102 78-79 -0.09931741252583137 1102 80-81 0.1403556543556519 1102 82-83 0.16456883695032332 1102 84-85 0.15771716035458994 1102 86-87 -0.05274863713633238 1102 88-89 0.1818314609475209 1102 90-91 0.3306766284699272 1102 92-93 0.46267889520878214 1102 94-95 0.3675249839272823 1102 96-97 0.48192612169664173 1102 98-99 0.2866085274463508 1102 100-101 0.5176930302884166 1103 1 0.12808449449311254 1103 2 0.21446441406646244 1103 3 0.06516342886247628 1103 4 0.05085937415717012 1103 5 0.11032052732182507 1103 6 0.1057955908448065 1103 7 0.10557585843817918 1103 8 0.13965998070814578 1103 9 0.12156933978542384 1103 10-11 0.11915219525081255 1103 12-13 0.1292446638055864 1103 14-15 0.20094918834944764 1103 16-17 0.07384644021468745 1103 18-19 0.23706080917744288 1103 20-21 0.17910636874692898 1103 22-23 0.11518076162079183 1103 24-25 0.1461283262837867 1103 26-27 0.19860421988683896 1103 28-29 0.23931449326212118 1103 30-31 0.16691473711467353 1103 32-33 0.15362452260153958 1103 34-35 0.2906472885226208 1103 36-37 0.3386514016767066 1103 38-39 0.5910198781160787 1103 40-41 0.2983930383537654 1103 42-43 0.25519048974761205 1103 44-45 0.38959997575691574 1103 46-47 0.34632011175173005 1103 48-49 0.5492992981062059 1103 50-51 0.3619686336920509 1103 52-53 0.4695994097081524 1103 54-55 0.4604187437928928 1103 56-57 0.3214653987781162 1103 58-59 0.1250945633043159 1103 60-61 0.3538180090673464 1103 62-63 0.5245477798031928 1103 64-65 0.4421185793154834 1103 66-67 0.4724043851864508 1103 68-69 0.3475932931847687 1103 70-71 0.24507860935779746 1103 72-73 0.2587688965055044 1103 74-75 0.1966487823487455 1103 76-77 0.1363612936601868 1103 78-79 0.10920294190082913 1103 80-81 0.28314587639281186 1103 82-83 0.2116170164415827 1103 84-85 0.24558747742806375 1103 86-87 0.3634087606322751 1103 88-89 0.6345299227309198 1103 90-91 0.7704890052350173 1103 92-93 0.6311341264923875 1103 94-95 0.5702839150137109 1103 96-97 0.7843040196305822 1103 98-99 1.1876386488136 1103 100-101 1.585590872376276 1104 1 0.06522117152433893 1104 2 0.0657908117461119 1104 3 -4.08301691678048E-4 1104 4 -0.05645378719816563 1104 5 0.12875398314544384 1104 6 0.02546204038822708 1104 7 -0.06281550415353365 1104 8 -0.024051966395717272 1104 9 0.11902991962737275 1104 10-11 0.13237622956447836 1104 12-13 0.13247539616637027 1104 14-15 0.2938892008852463 1104 16-17 0.1361697715406578 1104 18-19 0.17035148598744598 1104 20-21 0.2609124141953316 1104 22-23 0.19751450871728338 1104 24-25 0.11583118493656741 1104 26-27 0.19210588518112104 1104 28-29 0.17193997410816309 1104 30-31 0.19640688380804505 1104 32-33 0.08621543783744556 1104 34-35 0.1799267261324644 1104 36-37 0.2060385180752462 1104 38-39 0.2616372660005979 1104 40-41 0.3418005315635426 1104 42-43 0.42793788734122273 1104 44-45 0.5401585560167277 1104 46-47 0.16070893031454148 1104 48-49 0.3921233238168753 1104 50-51 0.7059433101579344 1104 52-53 0.5790902045021085 1104 54-55 0.37595805948598837 1104 56-57 0.17003422889352038 1104 58-59 0.19700101800954428 1104 60-61 0.3187607991419483 1104 62-63 0.22874757824727965 1104 64-65 0.5516861865764184 1104 66-67 0.3219249217676179 1104 68-69 0.44120003793386786 1104 70-71 0.4114375140734694 1104 72-73 0.4405939914457129 1104 74-75 0.4379843360744573 1104 76-77 0.4379738945917673 1104 78-79 0.39620581161459256 1104 80-81 0.44905158755335606 1104 82-83 0.22551239171657045 1104 84-85 0.3356193699643484 1104 86-87 0.5398101272796865 1104 88-89 0.6209579279418804 1104 90-91 0.4222489324782508 1104 92-93 0.6525509785331387 1104 94-95 0.8003787323229403 1104 96-97 0.8318025448312127 1104 98-99 0.582692079565625 1104 100-101 0.9062253126284681 1105 1 -0.007007957964614064 1105 2 -0.010975565116034147 1105 3 0.02089706648234113 1105 4 -0.10082875806988056 1105 5 -0.01007180489685311 1105 6 -0.05951813734317568 1105 7 -0.0880748022783493 1105 8 -0.11417450815628172 1105 9 -0.004999450705525987 1105 10-11 -0.05460368958291184 1105 12-13 -0.08595555513715425 1105 14-15 -0.10011202588541579 1105 16-17 -0.046712267544620545 1105 18-19 -0.18375751920077477 1105 20-21 -0.2473096553487366 1105 22-23 -0.23617297446347862 1105 24-25 -0.22164274994536726 1105 26-27 -0.31075169366069844 1105 28-29 -0.21886841664234424 1105 30-31 -0.27805982425210374 1105 32-33 -0.32216839705365174 1105 34-35 -0.28120899890252815 1105 36-37 -0.400536431448252 1105 38-39 -0.36015843458918795 1105 40-41 -0.5132612504711886 1105 42-43 -0.707051235592921 1105 44-45 -0.6943594779630118 1105 46-47 -0.5602755381073976 1105 48-49 -0.7993382170732275 1105 50-51 -0.7503894423636339 1105 52-53 -0.8954580627595021 1105 54-55 -0.7685652213437706 1105 56-57 -0.8624928585334359 1105 58-59 -0.797309006180086 1105 60-61 -0.8751700687231292 1105 62-63 -1.0113781206436698 1105 64-65 -0.7406870132954921 1105 66-67 -0.7188358865178941 1105 68-69 -0.6059441872988209 1105 70-71 -0.6285186018142639 1105 72-73 -0.841947378237716 1105 74-75 -0.97928456574029 1105 76-77 -0.8276219536314464 1105 78-79 -1.122081866105539 1105 80-81 -0.9409085123322356 1105 82-83 -0.8287056848092611 1105 84-85 -0.7282373504386825 1105 86-87 -0.6033176267527942 1105 88-89 -0.610533771060318 1105 90-91 -0.576113494062092 1105 92-93 -0.5318697857630248 1105 94-95 -0.3900379487512673 1105 96-97 -0.4343063370785778 1105 98-99 -0.6766000316675935 1105 100-101 -0.3853315435311693 1106 1 -0.0793114370709489 1106 2 -0.10637346084321564 1106 3 -0.13058106307290984 1106 4 0.0014949907297392429 1106 5 -0.07736853492940554 1106 6 -0.18154229018834656 1106 7 -0.09513323981907718 1106 8 -0.07321339810218319 1106 9 -0.04827592003356784 1106 10-11 -0.034657217752993574 1106 12-13 -0.02073298551532332 1106 14-15 -0.14777651676855186 1106 16-17 -0.13064160676460546 1106 18-19 -0.07231315748637002 1106 20-21 -0.15697206682800413 1106 22-23 -0.12790277480775814 1106 24-25 -0.08576838908490458 1106 26-27 -0.13148775468850005 1106 28-29 -0.06575620224202083 1106 30-31 -0.08452657551315923 1106 32-33 0.08365618096069483 1106 34-35 0.009999302533131527 1106 36-37 0.04970993596203499 1106 38-39 -0.011384503841718185 1106 40-41 0.10265390445788825 1106 42-43 -0.01229738577441708 1106 44-45 0.010100753438081256 1106 46-47 0.10019473975070525 1106 48-49 0.02744691668726773 1106 50-51 0.2097337188695647 1106 52-53 0.18562408118378926 1106 54-55 0.2457029648257958 1106 56-57 0.07779094713519186 1106 58-59 0.12898526542181799 1106 60-61 -0.0021058394134243485 1106 62-63 -0.09764154575741202 1106 64-65 0.0637437798592515 1106 66-67 -0.07655814527783278 1106 68-69 -0.1728315534222169 1106 70-71 -0.1748523790933305 1106 72-73 -0.12946316477821895 1106 74-75 0.04228213323603924 1106 76-77 0.03426997429653866 1106 78-79 0.09332707083669334 1106 80-81 -0.02820826858655323 1106 82-83 0.17507671786100332 1106 84-85 0.3665612934544171 1106 86-87 0.46651581119991903 1106 88-89 0.45722550648167015 1106 90-91 0.4427606915172113 1106 92-93 0.3059363382910938 1106 94-95 0.27207839898857955 1106 96-97 0.24462314446371636 1106 98-99 0.5887101036284861 1106 100-101 1.040022277223617 1107 1 -0.09157577806388773 1107 2 -0.1190149738839601 1107 3 -0.08580640315269505 1107 4 0.029693931707093668 1107 5 -0.06515271899525032 1107 6 -0.056852005052164145 1107 7 -0.05065414162053372 1107 8 -0.06152988735829723 1107 9 -0.05841618686257277 1107 10-11 -0.1369072365013153 1107 12-13 -0.10799280485505847 1107 14-15 -0.09690828583043753 1107 16-17 0.12325510250118299 1107 18-19 0.04009955254993969 1107 20-21 0.018600050967314985 1107 22-23 0.042454369624877586 1107 24-25 -0.05692612346460635 1107 26-27 0.0891821558048278 1107 28-29 0.11352293595293617 1107 30-31 -0.04808903535131748 1107 32-33 0.08035868264804691 1107 34-35 -0.10775741340852818 1107 36-37 -0.010154546782899843 1107 38-39 -0.060133439598956784 1107 40-41 -0.10500651129893868 1107 42-43 -0.06024014385052823 1107 44-45 -0.329442316962961 1107 46-47 -0.4068145579446494 1107 48-49 -0.5158120703741815 1107 50-51 -0.4322980253416162 1107 52-53 -0.18107574427304485 1107 54-55 -0.3640335146958975 1107 56-57 -0.3531850976607913 1107 58-59 -0.3347415179108566 1107 60-61 -0.2134126192464052 1107 62-63 -0.4146252428081212 1107 64-65 -0.4697668169842686 1107 66-67 -0.4499235492636515 1107 68-69 -0.15081845459725685 1107 70-71 -0.34607341661790514 1107 72-73 -0.2473320030147903 1107 74-75 -0.16608189587311628 1107 76-77 -0.32201424138411383 1107 78-79 -0.39578383244992565 1107 80-81 -0.04698746633498985 1107 82-83 -0.058423291696961144 1107 84-85 -0.24188044927160135 1107 86-87 -0.16919738217537628 1107 88-89 0.11548594194260886 1107 90-91 0.1460093706798169 1107 92-93 0.06991081999199977 1107 94-95 0.04714490037595809 1107 96-97 0.07474536879359306 1107 98-99 0.1968287135767106 1107 100-101 0.42935610168936833 1108 1 -0.15862299370691701 1108 2 -0.09705926126318332 1108 3 -0.1672192382216835 1108 4 -0.04814734052118297 1108 5 -0.009440515946678829 1108 6 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-1.1036211878913313 2316 70-71 -1.2884202115590853 2316 72-73 -1.1708843435537837 2316 74-75 -1.2686981431219984 2316 76-77 -1.110099865355597 2316 78-79 -1.1439558166749002 2316 80-81 -1.3054508873678259 2316 82-83 -1.3909154656429763 2316 84-85 -1.435705464880904 2316 86-87 -1.8113361957951284 2316 88-89 -2.07730681118338 2316 90-91 -2.864305915794425 2316 92-93 -2.694220871750449 2316 94-95 -2.8175128293926477 2316 96-97 -3.2243401493832167 2316 98-99 -3.364220317056656 2316 100-101 -3.7818498169416905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 28.0 5 99.0 6 362.0 7 686.0 8 1030.0 9 1594.0 10 2608.0 11 3967.0 12 5674.0 13 7983.0 14 8714.0 15 10988.0 16 13979.0 17 18959.0 18 16745.0 19 10495.0 20 8909.0 21 9086.0 22 9839.0 23 10908.0 24 12215.0 25 14099.0 26 16549.0 27 19553.0 28 23485.0 29 27917.0 30 33250.0 31 39856.0 32 48345.0 33 61163.0 34 84188.0 35 140599.0 36 154449.0 37 205591.0 38 151674.0 39 15778.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.975188082055936 17.85812685522758 13.352920211416954 16.813764851299535 2 13.727071814857187 25.77298819942873 39.027919653041884 21.472020332672198 3 24.153945456666747 32.19544008937512 28.993111367209366 14.657503086748761 4 10.920837379751095 17.23232579279711 38.977641950573414 32.86919487687838 5 10.460867747819131 43.970494794704976 31.66278182582763 13.905855631648262 6 23.63589206829374 36.84776432096143 20.447244924596035 19.069098686148784 7 24.084530331555847 39.07265757527176 20.635513516644103 16.207298576528288 8 23.429577222438127 30.97123259270948 21.333777634899945 24.26541254995245 9 27.431816636631545 20.840653257600618 22.476902893527942 29.250627212239895 10-11 22.891085402427265 27.38754036275488 26.454213296647676 23.26716093817018 12-13 31.53192550170684 25.409922753640096 26.03004239610604 17.028109348547023 14-15 26.429980358911514 25.895390219745167 31.193699743154994 16.480929678188318 16-17 17.4947255236809 30.115360676811736 33.091227140963134 19.29868665854423 18-19 16.560683307285153 31.64581133507137 35.26534730254444 16.528158055099038 20-21 19.23927905635719 28.88600894926317 32.34610532465211 19.528606669727537 22-23 17.820843507083783 28.303366759471977 31.141916579303214 22.733873154141026 24-25 19.314527737931463 28.73282563205955 33.15776706567073 18.794879564338252 26-27 21.611465345063692 25.359047458769503 35.669808537399646 17.35967865876715 28-29 17.789745195289843 27.29978520761166 37.34080476219654 17.569664834901957 30-31 17.3001041646557 31.08664468101358 34.63344701074298 16.979804143587746 32-33 16.95181146616999 30.59020482917752 33.45695716658707 19.001026538065425 34-35 18.7910015135146 27.38147703243689 33.910407994177625 19.917113459870876 36-37 20.705080659142034 27.291044108887636 35.21486927178632 16.789005960184006 38-39 21.986795272153387 25.565083442463315 35.96920016117659 16.478921124206707 40-41 18.08612263468309 28.29696342919117 37.01167989335822 16.605234042767517 42-43 16.59959247655481 31.2217099616609 33.980404459674695 18.198293102109595 44-45 17.43310552530986 31.216456233831423 31.417949660638232 19.93248858022049 46-47 17.80952940534597 30.05283855073802 30.011163665207558 22.126468378708456 48-49 19.531694927275602 29.334893986519013 30.90882307640332 20.224588009802066 50-51 20.638681122768023 30.492488943466256 31.700670736467128 17.16815919729859 52-53 17.976586874246152 31.194838267794655 32.65662679423829 18.1719480637209 54-55 15.728988914563999 30.159361011530294 33.84914842666044 20.26250164724526 56-57 18.951798036399715 31.789021666416257 29.684492728199075 19.574687568984952 58-59 18.839628429959618 29.782260359231376 30.5502792147238 20.8278319960852 60-61 18.415417828490465 28.42700948652564 32.67701829987896 20.48055438510493 62-63 22.077577067995765 27.781578216241126 31.409840496297157 18.731004219465948 64-65 20.177264574028673 29.60005304759259 32.615330250641485 17.60735212773726 66-67 17.725742402228025 29.586928986737103 33.06288815639823 19.62444045463664 68-69 17.098612817244664 32.36014123218492 31.48425994746391 19.056986003106505 70-71 17.908003525412347 31.91656524111302 29.098501699752383 21.076929533722247 72-73 17.95279100003945 29.70929583517643 29.021481756916124 23.316431407868 74-75 18.03139712418257 31.401362953810825 30.00424716484959 20.562992757157016 76-77 19.7557511402299 28.290514236647745 31.728742699356964 20.224991923765387 78-79 17.939279184343494 28.236879614255095 33.64893771109856 20.17490349030285 80-81 17.293060792818427 31.89315773901329 31.54860005078134 19.26518141738694 82-83 16.96406613154628 31.043039895650686 30.19574729537017 21.797146677432867 84-85 17.621117642269528 28.87832879938693 30.281362080875756 23.21919147746778 86-87 19.06320834455897 29.113741563961742 30.949505868922973 20.87354422255632 88-89 20.13938626087008 26.89636094237306 31.714374376574483 21.249878420182373 90-91 17.771760607332073 27.835337895590495 33.083631047196675 21.309270449880753 92-93 16.593068727688745 30.850952212680554 31.758175162814982 20.79780389681572 94-95 16.306818467964096 31.213540241501047 30.25150623393672 22.228135056598138 96-97 15.874534049923492 29.381651408489123 29.504617742573124 25.23919679901426 98-99 17.955941848948278 28.914032466551394 29.32040994476993 23.809615739730397 100-101 19.20189495808194 28.731286281698747 30.352274520468836 21.71454423975047 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15185.0 1 9851.0 2 9479.0 3 11959.5 4 7248.5 5 5600.5 6 8739.0 7 15169.0 8 19569.5 9 18142.5 10 12522.5 11 7705.5 12 6456.5 13 6230.0 14 5901.5 15 5363.0 16 4742.0 17 4391.0 18 4342.5 19 4426.5 20 4546.0 21 4783.0 22 5155.5 23 5633.0 24 6040.0 25 6530.5 26 7463.5 27 8580.5 28 9510.0 29 10476.0 30 11734.0 31 12966.5 32 14567.0 33 16163.5 34 17656.0 35 19102.0 36 20554.0 37 22211.5 38 22822.5 39 22520.5 40 22014.0 41 21932.5 42 22079.0 43 22396.5 44 25608.0 45 28223.0 46 32976.0 47 53544.0 48 136044.5 49 134779.0 50 62336.5 51 44856.5 52 25528.5 53 17467.5 54 14592.0 55 13205.0 56 12491.0 57 11586.0 58 10839.5 59 10349.5 60 9798.5 61 9383.0 62 8052.5 63 5081.5 64 2709.5 65 1936.0 66 1412.5 67 1034.0 68 819.0 69 639.5 70 548.0 71 469.0 72 413.5 73 495.5 74 427.5 75 297.0 76 218.0 77 117.0 78 84.0 79 62.0 80 38.5 81 25.0 82 15.0 83 5.0 84 2.5 85 1.5 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38056611517874606 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.5180819266348435E-4 16-17 0.0027279220871877473 18-19 8.393606422116146E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.2590409633174218E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009736583449654728 36-37 0.019179390674535394 38-39 0.012002857183626089 40-41 0.008813286743221953 42-43 0.003399410600957039 44-45 0.002434145862413682 46-47 0.0015108491559809062 48-49 1.2590409633174218E-4 50-51 5.036163853269687E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.036163853269687E-4 60-61 7.554245779904531E-4 62-63 0.0 64-65 0.0 66-67 0.0011751048990962605 68-69 9.652647385433568E-4 70-71 0.002769890119298328 72-73 0.0 74-75 0.0 76-77 0.0975756746571002 78-79 0.03697383628942162 80-81 4.196803211058073E-5 82-83 0.0 84-85 0.0 86-87 0.1485248656393452 88-89 0.3266371939166498 90-91 0.27711491602616456 92-93 0.0 94-95 8.393606422116146E-5 96-97 0.0 98-99 2.5180819266348435E-4 100-101 9.232967064327761E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1191383.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.663729060373065 #Duplication Level Percentage of deduplicated Percentage of total 1 76.61388079277873 27.323366868573807 2 12.866467773734644 9.177324412929876 3 4.405378071837607 4.713366298875754 4 2.0591293553527676 2.93744925718247 5 1.103430448531228 1.9676222276691822 6 0.6650911077150398 1.4231777439607547 7 0.4312768523804765 1.076665856931548 8 0.29125680134447535 0.8309842920112217 9 0.22250036400289974 0.7141673427870421 >10 1.1335005828423454 7.501623023076423 >50 0.10792413781961056 2.6819506263175747 >100 0.07194858162139699 5.111029614071775 >500 0.010110421597262022 2.636057274229135 >1k 0.013167060684806355 10.02416206501779 >5k 0.002351260836572564 5.444939145804823 >10k+ 0.00258638692022982 16.436113950560824 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 32063 2.6912420271230997 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 30277 2.5413322164241055 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21356 1.792538587507124 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 16949 1.4226323524844655 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16675 1.3996338708878673 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16353 1.3726064582086532 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13824 1.1603321517933358 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13554 1.1376694144536226 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12874 1.0805928907832325 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 11156 0.9363907324512772 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 10926 0.9170854376804101 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 9138 0.7670077548529733 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 8086 0.6787070152923115 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7220 0.6060183836767857 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6918 0.5806696922819949 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6160 0.5170461556023546 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5747 0.4823805610790149 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5743 0.4820448168221302 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5386 0.4520796418951756 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5306 0.4453647567574827 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5229 0.43890167981245326 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4844 0.4065862950873061 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4798 0.4027252361331327 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4687 0.3934083330045838 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4412 0.37032591534376436 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4023 0.33767478636173254 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3981 0.33414947166444375 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3943 0.3309599012240396 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3710 0.311402798260509 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3671 0.30812929175588366 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3333 0.2797589020491311 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3172 0.26624519570952415 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3069 0.2575997810947445 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2681 0.22503258817693386 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2578 0.21638717356215423 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2540 0.2131976031217501 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2480 0.20816143926848044 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2311 0.19397624441510414 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2258 0.18952763301138256 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2258 0.18952763301138256 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2180 0.18298062000213197 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2148 0.18029466594705482 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2091 0.17551031028644862 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2070 0.17374765293780423 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2001 0.16795606450654407 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1927 0.16174479575417813 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1926 0.16166085968995697 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1837 0.1541905499742736 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1785 0.1498258746347732 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1751 0.14697204845125372 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1735 0.14562907142371512 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1728 0.14504151897416698 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1593 0.1337101503043102 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1574 0.13211536508410815 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1507 0.12649164878129032 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1458 0.12237878163445341 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1457 0.12229484557023224 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1428 0.11986069970781857 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1419 0.11910527512982812 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1390 0.11667112926741442 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1366 0.11465666372610654 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1352 0.11348155882701029 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1326 0.1112992211572601 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1285 0.10785784252419248 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1285 0.10785784252419248 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1212 0.10173050983604769 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.393606422116146E-5 0.0 10-11 0.0 0.0 0.0 8.393606422116146E-5 0.0 12-13 0.0 0.0 0.0 1.259040963317422E-4 0.0 14-15 0.0 0.0 0.0 3.3574425688464584E-4 0.0 16-17 0.0 0.0 0.0 7.134565458798725E-4 0.0 18-19 0.0 0.0 0.0 0.0012170729312068413 0.0 20-21 0.0 8.393606422116146E-5 0.0 0.002056433573418456 0.0 22-23 0.0 8.393606422116146E-5 0.0 0.004154835178947492 0.0 24-25 0.0 8.393606422116146E-5 0.0 0.008183766261563242 0.0 26-27 0.0 8.393606422116146E-5 0.0 0.012590409633174218 0.0 28-29 0.0 8.393606422116146E-5 0.0 0.023921778303031015 0.0 30-31 0.0 8.393606422116146E-5 0.0 0.05057147869324978 0.0 32-33 0.0 8.393606422116146E-5 0.0 0.09845700333142238 0.0 34-35 0.0 8.393606422116146E-5 0.0 0.17844807253418926 0.0 36-37 0.0 8.393606422116146E-5 0.0 0.2924752157786371 0.0 38-39 0.0 8.393606422116146E-5 0.0 0.42324760383520665 0.0 40-41 0.0 8.393606422116146E-5 0.0 0.5710590129286719 0.0 42-43 0.0 1.259040963317422E-4 0.0 0.7157647876459543 0.0 44-45 0.0 1.6787212844232292E-4 0.0 0.871256346615656 0.0 46-47 0.0 1.6787212844232292E-4 0.0 1.067330992636289 0.0 48-49 0.0 1.6787212844232292E-4 0.0 1.2938324619370933 0.0 50-51 0.0 1.6787212844232292E-4 0.0 1.5207955795911139 0.0 52-53 0.0 1.6787212844232292E-4 0.0 1.73949099491935 0.0 54-55 0.0 1.6787212844232292E-4 0.0 1.965614751931159 0.0 56-57 0.0 1.6787212844232292E-4 0.0 2.22946776981038 0.0 58-59 0.0 1.6787212844232292E-4 0.0 2.5940020967228845 0.0 60-61 0.0 1.6787212844232292E-4 0.0 3.0797820684028565 0.0 62-63 0.0 1.6787212844232292E-4 0.0 3.4937547371416247 0.0 64-65 0.0 1.6787212844232292E-4 0.0 3.81065534760862 0.0 66-67 0.0 1.6787212844232292E-4 0.0 4.169062341832979 0.0 68-69 0.0 1.6787212844232292E-4 0.0 4.574893212342294 0.0 70-71 0.0 1.6787212844232292E-4 0.0 5.042668898246827 0.0 72-73 0.0 1.6787212844232292E-4 0.0 5.540241886949873 0.0 74-75 0.0 1.6787212844232292E-4 0.0 6.012676024418679 0.0 76-77 0.0 1.6787212844232292E-4 0.0 6.446625476442085 0.0 78-79 0.0 1.6787212844232292E-4 0.0 6.962748335338006 0.0 80-81 0.0 1.6787212844232292E-4 0.0 7.5280577278675285 0.0 82-83 0.0 1.6787212844232292E-4 0.0 8.111203534044048 0.0 84-85 0.0 1.6787212844232292E-4 0.0 8.864823486653746 0.0 86-87 0.0 1.6787212844232292E-4 0.0 9.657641581254726 0.0 88-89 0.0 1.6787212844232292E-4 0.0 10.291568706285048 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACACG 25 0.00504432 56.934223 5 TACCTGG 1005 0.0 50.041687 2 TCACGCA 70 7.993731E-8 47.44519 4 TATCACG 70 7.993731E-8 47.44519 2 ATAACGC 95 3.8926373E-10 44.94807 3 ATCACGC 80 2.2833046E-7 41.514538 3 ACCTGGG 1260 0.0 38.40801 3 GTACCCG 65 1.3061996E-4 36.642395 1 TATAACG 95 8.779225E-7 34.95961 2 AGTACTC 745 0.0 34.389805 5 GGATATG 355 0.0 29.520353 1 AAGTACT 875 0.0 29.280458 4 CTGGTCG 185 2.9467628E-10 28.211838 9 TACACGG 140 5.8652586E-7 27.111538 2 CCTGGGA 1390 0.0 26.96525 4 AAAGTAC 940 0.0 26.75101 3 GTGTAGC 250 1.4551915E-11 24.770258 1 ATATGAG 375 0.0 24.038897 3 CATGGGG 2835 0.0 23.764433 4 ATTAGAC 100 0.0016648311 23.722593 3 >>END_MODULE