##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938706_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1190850 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.620576059117436 33.0 31.0 34.0 30.0 34.0 2 31.527309064953606 33.0 31.0 34.0 30.0 34.0 3 31.533261955745896 33.0 31.0 34.0 28.0 34.0 4 35.076431960364445 37.0 35.0 37.0 32.0 37.0 5 35.16934878448167 37.0 35.0 37.0 33.0 37.0 6 35.308866775832385 37.0 35.0 37.0 33.0 37.0 7 35.26707897720116 37.0 35.0 37.0 33.0 37.0 8 35.26262081706344 37.0 35.0 37.0 33.0 37.0 9 36.75218373430743 39.0 37.0 39.0 32.0 39.0 10-11 36.63310240584457 39.0 37.0 39.0 32.0 39.0 12-13 36.51792291220556 39.0 37.0 39.0 32.0 39.0 14-15 37.41603182600663 40.0 37.0 41.0 32.0 41.0 16-17 37.36429861023639 40.0 37.0 41.0 32.0 41.0 18-19 37.508396943359784 40.0 37.0 41.0 32.0 41.0 20-21 37.42410379140949 40.0 37.0 41.0 32.0 41.0 22-23 37.385714405676616 40.0 36.5 41.0 32.0 41.0 24-25 37.2701990175085 40.0 36.0 41.0 32.0 41.0 26-27 36.956469748498975 39.5 35.5 41.0 31.0 41.0 28-29 36.70735818952849 39.0 35.0 41.0 30.5 41.0 30-31 36.41861359533107 39.0 35.0 41.0 30.0 41.0 32-33 36.0860322458748 38.0 35.0 40.5 30.0 41.0 34-35 35.718557332997435 38.0 35.0 40.0 29.5 41.0 36-37 35.34607633203174 38.0 35.0 40.0 27.5 41.0 38-39 35.03400134357812 38.0 35.0 40.0 26.5 41.0 40-41 34.809719528068186 38.0 35.0 40.0 25.5 41.0 42-43 34.75810849393291 38.0 35.0 40.0 24.5 41.0 44-45 34.476313137674765 38.0 34.5 40.0 23.5 41.0 46-47 33.92939664945207 38.0 33.5 40.0 21.0 41.0 48-49 33.49571356594029 38.0 33.0 40.0 16.5 41.0 50-51 32.27709325271864 36.5 31.5 39.0 12.5 40.0 52-53 32.293604988033756 36.5 32.0 39.0 9.0 40.0 54-55 32.79244363269933 37.0 33.0 40.0 8.0 41.0 56-57 32.76364907419071 37.0 33.0 40.0 6.5 41.0 58-59 32.61762270647017 37.0 33.0 40.0 2.0 41.0 60-61 32.2237741949028 36.0 32.5 40.0 2.0 41.0 62-63 31.729498677415293 35.5 31.5 39.0 2.0 41.0 64-65 31.229870260738128 35.0 31.0 39.0 2.0 40.5 66-67 30.780226308938992 35.0 30.0 38.5 2.0 40.0 68-69 30.462332367636563 35.0 30.0 38.0 2.0 40.0 70-71 29.941647142797162 35.0 29.5 37.0 2.0 39.0 72-73 29.401921316706556 35.0 29.0 36.5 2.0 39.0 74-75 28.981201242809757 34.5 29.0 36.0 2.0 38.5 76-77 28.438442289121216 34.0 27.5 35.0 2.0 37.5 78-79 27.99103371541336 34.0 26.5 35.0 2.0 37.0 80-81 27.570088172313895 34.0 26.0 35.0 2.0 36.5 82-83 27.182091783180084 34.0 26.0 35.0 2.0 36.0 84-85 26.851260024352353 34.0 25.0 35.0 2.0 36.0 86-87 26.343790569761097 33.0 24.0 35.0 2.0 35.0 88-89 25.73771885627913 33.0 21.5 35.0 2.0 35.0 90-91 25.150074316664565 32.5 18.5 35.0 2.0 35.0 92-93 24.737105848763488 32.0 11.0 35.0 2.0 35.0 94-95 24.27828735776966 32.0 2.0 35.0 2.0 35.0 96-97 23.533517655456187 32.0 2.0 35.0 2.0 35.0 98-99 22.748558592601924 31.0 2.0 35.0 2.0 35.0 100-101 20.497865390267457 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13681509685672566 1101 2 0.21150988447076458 1101 3 0.3110940628349539 1101 4 0.10125105965235548 1101 5 0.05692718150666565 1101 6 0.17474496596674527 1101 7 0.18060278994826717 1101 8 0.21871577758834349 1101 9 0.4286872635623027 1101 10-11 0.22889909584719703 1101 12-13 0.2893359341522128 1101 14-15 0.38467315389061696 1101 16-17 0.5943043348242227 1101 18-19 0.6656398077757686 1101 20-21 0.6542148864704487 1101 22-23 0.6034766926296129 1101 24-25 0.6764454921067227 1101 26-27 0.5481457969032562 1101 28-29 0.6413691793683256 1101 30-31 0.674309637243077 1101 32-33 0.6693215712378873 1101 34-35 0.6551390134802233 1101 36-37 0.691452507828977 1101 38-39 0.7098811780796623 1101 40-41 0.9497754612108906 1101 42-43 1.0532966431791877 1101 44-45 1.1196423455983933 1101 46-47 1.2201210626377232 1101 48-49 1.0476522960280192 1101 50-51 1.01816858355253 1101 52-53 1.1070013429601815 1101 54-55 1.2059768194424265 1101 56-57 1.2384626695063474 1101 58-59 1.1935890575163128 1101 60-61 1.2341555609102315 1101 62-63 1.0556494333295632 1101 64-65 0.8495941602950836 1101 66-67 0.9949314887425196 1101 68-69 1.1074393510824585 1101 70-71 1.0413695519837631 1101 72-73 0.8814201184019588 1101 74-75 0.8471754100625972 1101 76-77 0.923892141783444 1101 78-79 0.922349138569345 1101 80-81 0.8582898257069829 1101 82-83 1.0476162361802643 1101 84-85 0.9297478509929427 1101 86-87 0.7539303728779565 1101 88-89 0.6594913572657823 1101 90-91 0.7997328894781184 1101 92-93 0.7946075233308534 1101 94-95 0.8291186609286001 1101 96-97 0.7727502074031065 1101 98-99 0.7868656995116758 1101 100-101 0.8474070863543695 1102 1 0.13126092702384184 1102 2 0.1317227121377833 1102 3 0.0836616844901279 1102 4 0.28013013246853546 1102 5 0.027221073950094876 1102 6 0.22332582481832475 1102 7 0.10738563495937825 1102 8 0.2613226409760685 1102 9 0.39984255989104156 1102 10-11 0.3523796751050554 1102 12-13 0.2536467675387186 1102 14-15 0.2211246049356248 1102 16-17 0.510550571620378 1102 18-19 0.5074355158381607 1102 20-21 0.5521374384302149 1102 22-23 0.44326717136134874 1102 24-25 0.34260218060335035 1102 26-27 0.3536806069013352 1102 28-29 0.4382529710536005 1102 30-31 0.44366222116470055 1102 32-33 0.4837303639382924 1102 34-35 0.3873515308098092 1102 36-37 0.42935514888748827 1102 38-39 0.5725401897564737 1102 40-41 0.41507748913545583 1102 42-43 0.7053007123699402 1102 44-45 0.5871979453037213 1102 46-47 0.5897148106437342 1102 48-49 0.5864361358591026 1102 50-51 0.8519375735927284 1102 52-53 0.9091380306447121 1102 54-55 0.5404553075480933 1102 56-57 0.7834855242626304 1102 58-59 0.6755180656546926 1102 60-61 0.7789978688925743 1102 62-63 0.519857304298796 1102 64-65 0.5447122915417069 1102 66-67 0.7867654089132898 1102 68-69 0.3820016883003241 1102 70-71 0.42643666607772346 1102 72-73 0.16622540341933245 1102 74-75 0.215075353795811 1102 76-77 0.5044359428658574 1102 78-79 0.5413207615470768 1102 80-81 0.6888372522266195 1102 82-83 0.7080537974501517 1102 84-85 0.5538480174730189 1102 86-87 0.3419733908659204 1102 88-89 0.39490731092979203 1102 90-91 0.34749802495345605 1102 92-93 0.4118048573293329 1102 94-95 0.45875051953074575 1102 96-97 0.24761033803486399 1102 98-99 0.21752957507507276 1102 100-101 0.4437614191869059 1103 1 0.0101116674975259 1103 2 0.2581773130585212 1103 3 0.261028704291558 1103 4 0.2712832746283951 1103 5 0.20921258762250972 1103 6 0.27980150289253913 1103 7 0.36849967345291645 1103 8 0.24014980831757526 1103 9 0.31656700384245084 1103 10-11 0.30956046716229935 1103 12-13 0.3350219131374743 1103 14-15 0.3236497033881136 1103 16-17 0.29844299493836957 1103 18-19 0.40327347135427516 1103 20-21 0.2085042640999717 1103 22-23 0.3795476084349616 1103 24-25 0.4938862229795262 1103 26-27 0.4510730024862255 1103 28-29 0.4788881976330046 1103 30-31 0.5493419593642699 1103 32-33 0.4234677315127584 1103 34-35 0.31381098502247085 1103 36-37 0.3586718053853488 1103 38-39 0.5795469566320648 1103 40-41 0.5828786705653712 1103 42-43 0.5252803285438787 1103 44-45 0.48362800243389614 1103 46-47 0.16268197465726786 1103 48-49 0.16031363877108618 1103 50-51 0.14430057209514047 1103 52-53 0.3499833354314461 1103 54-55 0.39960168283236186 1103 56-57 0.24920325680464117 1103 58-59 0.2291462480275399 1103 60-61 0.16737770138467312 1103 62-63 0.27970602025641966 1103 64-65 0.09015574375758106 1103 66-67 0.018199487342489107 1103 68-69 -0.06518756235140089 1103 70-71 -0.04664697694185804 1103 72-73 0.028181834724453125 1103 74-75 0.06298289165148674 1103 76-77 0.15163559342883914 1103 78-79 0.12317915635543741 1103 80-81 0.3510918143210233 1103 82-83 0.12571076677734894 1103 84-85 0.150400793555999 1103 86-87 0.34162118053811596 1103 88-89 0.39975436290161426 1103 90-91 0.21537893903632366 1103 92-93 -0.0545410298524267 1103 94-95 0.1788972276121683 1103 96-97 0.10056044619393845 1103 98-99 0.4509181434926184 1103 100-101 0.603694721089397 1104 1 0.16982467410491608 1104 2 0.21793209454111206 1104 3 0.03999087714206695 1104 4 0.13595356642589707 1104 5 0.3288662739071171 1104 6 0.20621088274451438 1104 7 0.46960879638940867 1104 8 0.29474469646535084 1104 9 0.35836624771721404 1104 10-11 0.2651408942407727 1104 12-13 0.3664512404923599 1104 14-15 0.3136418357866191 1104 16-17 0.37195424282351297 1104 18-19 0.3462298792720091 1104 20-21 0.36000885585500697 1104 22-23 0.28926096316630634 1104 24-25 0.3094762582792896 1104 26-27 0.26149096011258166 1104 28-29 0.3373991322065848 1104 30-31 0.36403279047541304 1104 32-33 0.3270603707797477 1104 34-35 0.537890719334257 1104 36-37 0.3868406037310663 1104 38-39 0.3983198892979303 1104 40-41 0.20309596954119513 1104 42-43 0.3309032267454697 1104 44-45 0.5383398602322202 1104 46-47 0.5700830900464311 1104 48-49 0.45328257915137726 1104 50-51 0.42250313846496823 1104 52-53 0.5200331867825128 1104 54-55 0.36588657355624576 1104 56-57 0.45220721845530676 1104 58-59 0.5635200078359262 1104 60-61 0.25963542451471966 1104 62-63 0.4674336062952591 1104 64-65 0.4713278075060394 1104 66-67 0.4282202355304996 1104 68-69 0.4067136122188373 1104 70-71 0.5481092318489686 1104 72-73 0.35487389123193225 1104 74-75 0.31669284869298764 1104 76-77 0.5523130426549585 1104 78-79 0.3018635262858851 1104 80-81 0.3400054586915111 1104 82-83 0.31250363998363895 1104 84-85 0.453284304316508 1104 86-87 0.4932712881084207 1104 88-89 0.5623310372038013 1104 90-91 0.6907686638095214 1104 92-93 0.5965325844401477 1104 94-95 0.8796815508940057 1104 96-97 0.9020918434938885 1104 98-99 0.9482699645290573 1104 100-101 1.232907891315051 1105 1 0.24650651611807817 1105 2 0.33917854797824987 1105 3 0.24914090556990232 1105 4 0.23605413595097957 1105 5 0.18036160579347182 1105 6 0.3071027283128842 1105 7 0.16388297353841352 1105 8 0.30492338374946115 1105 9 0.19952104040150687 1105 10-11 0.28322750023372123 1105 12-13 0.39874173563472937 1105 14-15 0.3309330156870942 1105 16-17 0.39698076855450637 1105 18-19 0.2654204675138132 1105 20-21 0.40541696477442457 1105 22-23 0.2488401758466452 1105 24-25 0.19643341455493157 1105 26-27 0.3650364162929094 1105 28-29 0.4915454463404103 1105 30-31 0.5850453886413689 1105 32-33 0.6161029959348525 1105 34-35 0.5683136923464929 1105 36-37 0.6945577585126799 1105 38-39 0.5929485498142171 1105 40-41 0.5483572358872024 1105 42-43 0.6765905648070927 1105 44-45 0.7935786056446901 1105 46-47 0.991171979949776 1105 48-49 0.8694221895724183 1105 50-51 0.9886708308854608 1105 52-53 1.0557806728902221 1105 54-55 1.0890661890680207 1105 56-57 0.9563773262044251 1105 58-59 1.1479292414186801 1105 60-61 1.082702919531208 1105 62-63 1.1494796635410367 1105 64-65 0.9870426603937119 1105 66-67 0.98823029248301 1105 68-69 1.0609574525083438 1105 70-71 1.0302858081414001 1105 72-73 0.9538165970363295 1105 74-75 0.9832823936124271 1105 76-77 1.055243323212114 1105 78-79 1.0529183304519805 1105 80-81 1.112506679497642 1105 82-83 1.1055856463387492 1105 84-85 0.9659329625364457 1105 86-87 1.1053796790928523 1105 88-89 1.242767567043348 1105 90-91 1.2819777529705583 1105 92-93 1.3937314395799127 1105 94-95 1.5995923110031782 1105 96-97 1.4761639962489 1105 98-99 1.5074130070407747 1105 100-101 1.5057255533710148 1106 1 0.07008843621272831 1106 2 0.12716621811733475 1106 3 0.13366385295842775 1106 4 0.1289716282779736 1106 5 0.1647927689333386 1106 6 0.02243892128263525 1106 7 0.1453324749589413 1106 8 0.12030308139198098 1106 9 0.003992737163322602 1106 10-11 0.08621035567883695 1106 12-13 0.1491476023990188 1106 14-15 0.2853207537359168 1106 16-17 0.33706700861011285 1106 18-19 0.2809654298583908 1106 20-21 0.3284063989749413 1106 22-23 0.288912887010099 1106 24-25 0.27777853128804963 1106 26-27 0.2697127707074287 1106 28-29 0.280146558892838 1106 30-31 0.053343347900828064 1106 32-33 0.20553892071421132 1106 34-35 0.14191683423273815 1106 36-37 0.37432755512833893 1106 38-39 0.35570284656383677 1106 40-41 0.46663079008632735 1106 42-43 0.6278938480835237 1106 44-45 0.399677615913383 1106 46-47 0.44810434344723404 1106 48-49 0.4316623884206052 1106 50-51 0.4912718267180267 1106 52-53 0.44030163073578876 1106 54-55 0.21336354881187702 1106 56-57 0.3311362599155032 1106 58-59 0.44463243609413183 1106 60-61 0.5627988685564631 1106 62-63 0.5939992684973845 1106 64-65 0.5077073799610012 1106 66-67 0.4424654989047241 1106 68-69 0.4535977351826084 1106 70-71 0.540157920319789 1106 72-73 0.6452908748985529 1106 74-75 0.7354277841066583 1106 76-77 0.7031943592334535 1106 78-79 0.5656089618268041 1106 80-81 0.6131367014356712 1106 82-83 0.6565723270117978 1106 84-85 0.6195959764022305 1106 86-87 0.6832225370315292 1106 88-89 1.0106310101198694 1106 90-91 1.163610557616014 1106 92-93 1.1177009226435963 1106 94-95 1.3250386981257947 1106 96-97 1.266511927522128 1106 98-99 1.3451730734649807 1106 100-101 1.8247023446891788 1107 1 0.10556286430425388 1107 2 -0.026280942697805898 1107 3 0.039441981010419624 1107 4 0.09808451228862225 1107 5 0.048417216793247064 1107 6 -0.04454316672986636 1107 7 0.017876155229572532 1107 8 0.16290721887519055 1107 9 0.022656185031088683 1107 10-11 -0.0013963084916923663 1107 12-13 0.10919714118039536 1107 14-15 0.06523254223777997 1107 16-17 0.18179552718584802 1107 18-19 0.009663361116786007 1107 20-21 0.06751480564329171 1107 22-23 0.1630930227966374 1107 24-25 -0.11015961331177948 1107 26-27 0.08771993445509452 1107 28-29 0.11280196325444791 1107 30-31 0.02175713811508473 1107 32-33 0.05668430865890173 1107 34-35 0.023254171863619888 1107 36-37 -0.12039847603501386 1107 38-39 -0.21423795302141713 1107 40-41 -0.1291483329306118 1107 42-43 -0.07002955532501431 1107 44-45 0.0266259798506141 1107 46-47 -0.09031439540850528 1107 48-49 -0.010202856668058757 1107 50-51 -0.140278904712126 1107 52-53 -0.050759394654143364 1107 54-55 3.3369724493326203E-4 1107 56-57 0.07214819163996111 1107 58-59 0.1329007519588501 1107 60-61 0.10627425495633958 1107 62-63 0.12221241617374545 1107 64-65 0.15273661360233604 1107 66-67 0.027512746648380215 1107 68-69 0.1608528625250294 1107 70-71 0.21681525700807214 1107 72-73 0.050280231391877095 1107 74-75 0.14545807817116696 1107 76-77 0.11701861176603146 1107 78-79 0.17080228613563264 1107 80-81 -0.05757113757900001 1107 82-83 -0.1032496789946471 1107 84-85 0.2998777347671364 1107 86-87 0.16599196961348994 1107 88-89 0.23352279720784352 1107 90-91 0.2582088143326793 1107 92-93 0.28256399713767166 1107 94-95 0.32453728815572447 1107 96-97 0.3355323845252407 1107 98-99 0.421689805895344 1107 100-101 0.6636790494176203 1108 1 0.1843978850745529 1108 2 0.37058868660343336 1108 3 0.21931738799603195 1108 4 0.17674104953554348 1108 5 0.350398383902764 1108 6 0.1375676024477528 1108 7 0.27333357683356496 1108 8 0.3690225825641562 1108 9 0.4363723559261814 1108 10-11 0.4379106572596214 1108 12-13 0.3313249238680669 1108 14-15 0.48071339700113924 1108 16-17 0.6310810173390209 1108 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-2.4919171998548997 2316 98-99 -2.6922066940293696 2316 100-101 -3.0804450069753138 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6196.0 3 1348.0 4 1222.0 5 1402.0 6 1700.0 7 2056.0 8 2593.0 9 3098.0 10 3990.0 11 5482.0 12 8021.0 13 10321.0 14 10828.0 15 12585.0 16 15523.0 17 19473.0 18 16650.0 19 11383.0 20 10714.0 21 11318.0 22 12262.0 23 13271.0 24 14751.0 25 16592.0 26 18627.0 27 21200.0 28 24952.0 29 28858.0 30 34056.0 31 40654.0 32 49677.0 33 62294.0 34 85892.0 35 147406.0 36 148110.0 37 170953.0 38 126389.0 39 18984.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.443271467871135 16.958984554264692 12.727679973593292 16.870064004270883 2 13.285726301623349 24.81192699133653 40.171503070064766 21.730843636975354 3 24.051891757274195 32.60449067814507 28.667680225753877 14.675937338826857 4 10.580234920074187 16.41429370476443 39.765332256721464 33.24013911843992 5 10.667658746024038 43.44507973809778 31.65072634181118 14.236535174067 6 23.41186141074579 36.33428881763655 21.250705675778683 19.003144095838973 7 24.339045653387114 39.16273925373122 20.756352813133145 15.741862279748522 8 23.234661537360736 30.183299731889946 21.718766441207297 24.863272289542024 9 28.024226950211467 20.685079081548984 22.84387459643475 28.446819371804803 10-11 22.720944703398118 26.98082733069878 26.815147464757267 23.483080501145835 12-13 31.715889942379423 25.129052005780995 25.99418114193512 17.16087690990447 14-15 26.750589656367445 25.42150756879885 31.366900846884494 16.461001927949205 16-17 17.68450606728818 29.796176675793877 33.14189062586186 19.37742663105608 18-19 16.653052739134843 31.544374880851834 35.34280795356367 16.459764426449652 20-21 19.388228167082453 28.704097129611323 32.308216555954274 19.599458147351946 22-23 17.823424042236542 28.367492272571166 30.942881000962984 22.86620268422931 24-25 19.299420258627944 28.490862845042063 33.21136151688892 18.998355379441065 26-27 21.746864048816946 25.119628311738868 35.55768827292866 17.575819366515532 28-29 17.819395254984265 27.214494405352603 37.3160265052396 17.650083834423537 30-31 17.415040060904026 30.889592923275412 34.63558641179948 17.05978060402108 32-33 16.967811385377882 30.438283245842708 33.32410681947344 19.26979854930597 34-35 18.80122264368465 27.28830642612689 33.86882283886268 20.041648091325786 36-37 20.809860876690987 27.121289019358557 35.20577637416093 16.86307372978952 38-39 22.130896391917904 25.500853246995725 35.729870725388494 16.63837963569788 40-41 18.02685134355724 28.247837555564637 37.07093946840794 16.65437163247018 42-43 16.631258742231296 31.207326637786835 33.81940736364016 18.342007256341713 44-45 17.432810507211865 31.16224356386068 31.434895967239562 19.97004996168789 46-47 17.812254607943842 29.86753518155482 30.068632851966235 22.2515773585351 48-49 19.514737959509922 29.275416329755256 30.955279805804125 20.254565904930697 50-51 20.728321545371077 30.334492035642953 31.910543572743205 17.026642846242762 52-53 17.99187926134328 31.02679455930744 32.81758099450383 18.163745184845446 54-55 15.800564165857342 30.04735384968167 34.07099789575915 20.081084088701846 56-57 18.92492721813992 31.625340730364073 29.93053904934332 19.51919300215269 58-59 18.776135488902437 29.564612511159922 30.89364886289952 20.765603137038124 60-61 18.59093507065668 28.11828860509126 32.97527995427564 20.31549636997642 62-63 22.205335437297485 27.581275730571207 31.581462861998162 18.63192597013315 64-65 20.23594399945359 29.420608693525768 33.000454500610076 17.342992806410567 66-67 17.872796152978555 29.456909691551015 33.34824524972541 19.322048905745024 68-69 17.226314429066516 32.35301682717141 31.814641793807592 18.606026949954483 70-71 18.04763256062435 31.832470137412805 29.367059465435553 20.75283783652729 72-73 18.17684309939203 29.84476056361781 29.242480458044877 22.73591587894528 74-75 18.34753774903401 31.33716849043916 30.36394643146993 19.9513473290569 76-77 20.2035688195563 28.346195140601676 31.767982467418328 19.6822535724237 78-79 18.405349048509258 28.453694791369916 33.79604266157811 19.344913498542716 80-81 17.874998208744866 32.16032112779109 31.587498809342172 18.37718185412187 82-83 17.463564445913182 31.21781750862415 30.249771540139808 21.06884650532286 84-85 18.295301458946987 29.212745258380135 30.392227941709564 22.099725340963314 86-87 19.915448018023145 29.326513537379267 31.186446393158285 19.571592051439303 88-89 21.019563375472643 27.22993394568118 31.663160912030147 20.08734176681603 90-91 18.665312424371976 28.36433502571458 33.13791262860453 19.832439921308907 92-93 17.71597604196619 31.53451324597966 31.561791944899557 19.18771876715459 94-95 17.46871032526588 32.02912428545949 30.05558626581139 20.44657912346324 96-97 17.28050247001658 30.01247236094456 29.28850198427757 23.418523184761288 98-99 19.48861717418867 29.667995819009846 28.931895313336035 21.911491693465454 100-101 21.18509607075623 29.143612523468647 30.11291928471526 19.55837212105986 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15930.0 1 10159.5 2 9862.0 3 12548.0 4 7330.0 5 5539.5 6 8773.5 7 14942.5 8 19717.5 9 18635.0 10 12576.0 11 7879.0 12 6828.5 13 6498.5 14 6216.5 15 5653.5 16 4996.0 17 4733.5 18 4606.5 19 4530.5 20 4676.0 21 4964.5 22 5259.0 23 5752.5 24 6268.5 25 6951.5 26 7968.5 27 8983.5 28 9866.0 29 10794.5 30 12043.5 31 13210.0 32 14565.5 33 16163.0 34 17670.5 35 18974.5 36 20357.0 37 21876.0 38 22289.5 39 21811.0 40 21469.0 41 21321.0 42 21514.5 43 22280.5 44 25531.0 45 28279.0 46 34295.5 47 55293.0 48 134452.0 49 134024.5 50 60975.5 51 40518.0 52 23547.0 53 17418.5 54 15181.5 55 13511.0 56 12406.5 57 11333.0 58 10631.5 59 10212.0 60 9817.0 61 9536.0 62 8181.5 63 5293.5 64 2989.5 65 2102.0 66 1564.5 67 1167.5 68 899.5 69 739.0 70 634.5 71 537.0 72 481.5 73 503.0 74 438.0 75 340.0 76 274.0 77 190.5 78 134.0 79 95.0 80 76.0 81 55.5 82 39.0 83 32.0 84 29.5 85 24.5 86 11.5 87 6.5 88 6.5 89 6.5 90 5.0 91 1.5 92 1.5 93 2.0 94 1.0 95 0.5 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27484569845068646 2 0.16374858294495528 3 0.19305538061048833 4 0.16248897846076332 5 0.23285888231095436 6 0.19087206617122224 7 0.23789730024772224 8 0.24394340177184365 9 0.14787756644413652 10-11 0.20267036150648696 12-13 0.20762480581097537 14-15 0.1697946844690767 16-17 0.2834529957593315 18-19 0.2291220556745182 20-21 0.19607843137254902 22-23 0.24134021917118026 24-25 0.22958391065205525 26-27 0.3400092370995507 28-29 0.3097367426628039 30-31 0.28618213880841414 32-33 0.18864676491581642 34-35 0.3561321744972079 36-37 0.3953478607717177 38-39 0.3778393584414494 40-41 0.34063903934164674 42-43 0.3728009405046815 44-45 0.38132426418104715 46-47 0.3871604316244699 48-49 0.40567661754209183 50-51 0.3991266742242936 52-53 0.3989587269597346 54-55 0.3924927572742159 56-57 0.3718352437334677 58-59 0.3464332199689298 60-61 0.3137674770122182 62-63 0.3793928706386195 64-65 0.4142419280345972 66-67 0.41965822731662256 68-69 0.42486459251794934 70-71 0.4235210144014779 72-73 0.41482974346055346 74-75 0.41084099592727885 76-77 0.3959776630138137 78-79 0.36914808750052486 80-81 0.3804845278582525 82-83 0.3901414955703909 84-85 0.3906453373640677 86-87 0.3860687744048369 88-89 0.3950959398748793 90-91 0.4154175588865096 92-93 0.4150816643573918 94-95 0.4228492253432422 96-97 0.4222194231011462 98-99 0.42041399000713775 100-101 0.39408825628752575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1190850.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.35220615677271 #Duplication Level Percentage of deduplicated Percentage of total 1 78.06756117729145 29.159956392506565 2 12.166896360402797 9.089208422837057 3 4.1457774064051875 4.645617970924111 4 1.8411789560087413 2.750883837454002 5 1.0094521693275942 1.8852632767062865 6 0.621246810449033 1.3922963362877865 7 0.39189187792271357 1.02466183507338 8 0.2869381760092617 0.8574219123637021 9 0.1943434380366269 0.6533240546484064 >10 1.0848328994274374 7.412451709692 >50 0.09820716753426251 2.549428257666211 >100 0.06441925922508823 4.743918705305021 >500 0.010361139588035443 2.715978852639952 >1k 0.012163076907693781 9.420531072665904 >5k 0.0022524216495729225 5.441464724992076 >10k+ 0.0024776638145302147 16.257592638237607 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 31775 2.6682621656799763 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 28659 2.406600327497166 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22391 1.880253600369484 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 18533 1.556283327035311 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15838 1.3299743880421548 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15784 1.3254398118990638 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14364 1.2061972540622243 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12922 1.0851072763152372 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11563 0.9709871100474452 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 11082 0.9305957929210228 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 10326 0.8671117269177477 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 9062 0.760969055716505 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7573 0.6359323172523828 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 7474 0.6276189276567158 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7342 0.6165344081958265 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6179 0.5188730738548096 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5883 0.4940168787000882 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5433 0.4562287441743293 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5428 0.45580887601293196 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5172 0.4343116261493891 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5131 0.43086870722593107 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4838 0.40626443296804804 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4467 0.37511021539236683 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4421 0.37124742830751145 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4393 0.3688961666036864 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3984 0.3345509510013856 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3933 0.3302682957551329 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3826 0.3212831171012302 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3457 0.2902968467901079 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3248 0.27274635764369987 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3171 0.26628038795818115 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3006 0.25242473863206955 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2875 0.24142419280345973 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2595 0.21791157576520975 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2445 0.20531553092329008 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2189 0.18381828105974723 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2183 0.18331443926607047 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2162 0.1815509929882017 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2148 0.1803753621362892 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2116 0.17768820590334636 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2087 0.17525297056724187 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2065 0.17340555065709368 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1961 0.1646722929000294 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1952 0.1639165302095142 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1926 0.16173321577024816 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1879 0.15778645505311334 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1850 0.15535121971700885 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1849 0.1552672460847294 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1730 0.14527438384347313 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1671 0.14031993953898475 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1578 0.13251039173699458 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1575 0.13225847084015618 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1518 0.12747197380022673 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1464 0.12293739765713566 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1463 0.1228534240248562 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1405 0.11798295335264726 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1386 0.11638745433933745 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1379 0.11579963891338121 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1358 0.11403619263551244 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1353 0.11361632447411513 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1313 0.11025737918293657 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1308 0.10983751102153923 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1287 0.10807406474367048 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1275 0.10706638115631692 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1255 0.10538690851072763 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.397363227946425E-5 0.0 5 0.0 0.0 0.0 8.397363227946425E-5 0.0 6 0.0 0.0 0.0 8.397363227946425E-5 0.0 7 0.0 0.0 0.0 8.397363227946425E-5 0.0 8 0.0 0.0 0.0 8.397363227946425E-5 0.0 9 0.0 0.0 0.0 8.397363227946425E-5 0.0 10-11 0.0 0.0 0.0 1.679472645589285E-4 0.0 12-13 0.0 0.0 0.0 2.5192089683839276E-4 0.0 14-15 0.0 4.1986816139732125E-5 0.0 2.5192089683839276E-4 0.0 16-17 0.0 8.397363227946425E-5 0.0 7.137758743754461E-4 0.0 18-19 0.0 8.397363227946425E-5 0.0 0.0012176176680522316 0.0 20-21 0.0 8.397363227946425E-5 0.0 0.002015367174707142 0.0 22-23 0.0 8.397363227946425E-5 0.0 0.004576562959230802 0.0 24-25 0.0 8.397363227946425E-5 0.0 0.007851534618129907 0.0 26-27 0.0 8.397363227946425E-5 0.0 0.012680018474199101 0.0 28-29 0.0 8.397363227946425E-5 0.0 0.023218709325271862 0.0 30-31 0.0 8.397363227946425E-5 0.0 0.047948944031574084 0.0 32-33 0.0 8.397363227946425E-5 0.0 0.09295881093336693 0.0 34-35 0.0 8.397363227946425E-5 0.0 0.16853507998488476 0.0 36-37 0.0 8.397363227946425E-5 0.0 0.2726623840114204 0.0 38-39 0.0 8.397363227946425E-5 0.0 0.3991266742242936 0.0 40-41 0.0 8.397363227946425E-5 0.0 0.5431414535835748 0.0 42-43 0.0 8.397363227946425E-5 0.0 0.6750220430784734 0.0 44-45 0.0 8.397363227946425E-5 0.0 0.8182810597472394 0.0 46-47 0.0 8.397363227946425E-5 0.0 1.006423982869379 0.0 48-49 0.0 8.397363227946425E-5 0.0 1.2259730444640384 0.0 50-51 0.0 8.397363227946425E-5 0.0 1.439811899063694 0.0 52-53 0.0 8.397363227946425E-5 0.0 1.6457152454129402 0.0 54-55 0.0 8.397363227946425E-5 0.0 1.8632489398328924 0.0 56-57 0.0 8.397363227946425E-5 0.0 2.1212159381954065 0.0 58-59 0.0 8.397363227946425E-5 0.0 2.468320947222572 0.0 60-61 0.0 8.397363227946425E-5 0.0 2.940126800184742 0.0 62-63 0.0 8.397363227946425E-5 0.0 3.344333879161943 0.0 64-65 0.0 1.2596044841919638E-4 0.0 3.665365075366335 0.0 66-67 0.0 1.679472645589285E-4 0.0 4.023176722509132 0.0 68-69 0.0 1.679472645589285E-4 0.0 4.415795440231767 0.0 70-71 0.0 1.679472645589285E-4 0.0 4.892219842969308 0.0 72-73 0.0 1.679472645589285E-4 0.0 5.388210102027963 0.0 74-75 0.0 1.679472645589285E-4 0.0 5.8628710584876345 0.0 76-77 0.0 1.679472645589285E-4 0.0 6.29453751522022 0.0 78-79 0.0 1.679472645589285E-4 0.0 6.801696267372046 0.0 80-81 0.0 1.679472645589285E-4 0.0 7.350086072973086 0.0 82-83 0.0 1.679472645589285E-4 0.0 7.928244531217198 0.0 84-85 0.0 1.679472645589285E-4 0.0 8.667548389805601 0.0 86-87 0.0 1.679472645589285E-4 0.0 9.447159591888148 4.1986816139732125E-5 88-89 0.0 1.679472645589285E-4 0.0 10.069236259814419 8.397363227946425E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 50 4.274898E-7 56.93648 2 TATAACG 60 1.5038349E-6 47.447067 2 TACCTGG 850 0.0 45.214264 2 ACCTGGG 1060 0.0 34.91388 3 TCACGCA 90 2.4314877E-5 31.631378 4 AGTACTC 710 0.0 30.072084 5 ATCACGC 95 3.5176086E-5 29.966568 3 AAGTACT 755 0.0 29.536585 4 ATAACGC 100 4.991032E-5 28.46824 3 CATTCCG 135 4.2745705E-7 28.110859 9 CATGGGG 2810 0.0 27.184973 4 ATGGGGA 1140 0.0 26.63695 5 CAGTCGC 45 8.922747E-4 26.40286 58-59 CGCCGTA 75 1.0587464E-6 25.339262 92-93 TGGGAGT 820 0.0 24.878685 6 ATGGGAG 2815 0.0 24.776978 5 GATAGTC 115 1.2918962E-4 24.75395 7 TACGCAG 135 1.3544268E-5 24.602182 4 GGGATCG 135 1.354869E-5 24.601147 7 CATGGGT 755 0.0 24.50908 4 >>END_MODULE