##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938706_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1190850 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.990422807238527 31.0 31.0 34.0 30.0 34.0 2 32.102134609732545 33.0 31.0 34.0 30.0 34.0 3 32.17278666498719 34.0 31.0 34.0 30.0 34.0 4 35.773308141243646 37.0 35.0 37.0 35.0 37.0 5 35.72417852794223 37.0 35.0 37.0 33.0 37.0 6 35.83023890498384 37.0 35.0 37.0 35.0 37.0 7 35.766301381366254 37.0 35.0 37.0 33.0 37.0 8 35.80647184783978 37.0 35.0 37.0 33.0 37.0 9 37.3769164882227 39.0 37.0 39.0 34.0 39.0 10-11 37.291994373766634 39.0 37.0 39.0 33.5 39.0 12-13 37.20770835957509 39.0 37.0 39.0 33.0 39.0 14-15 38.149950455556954 40.0 37.0 41.0 33.0 41.0 16-17 38.12833606247638 40.0 37.5 41.0 33.0 41.0 18-19 38.4486219926943 40.0 38.0 41.0 34.0 41.0 20-21 38.45363269933241 40.0 38.0 41.0 34.0 41.0 22-23 38.33670067598774 40.0 37.5 41.0 34.0 41.0 24-25 38.187644959482725 40.0 37.0 41.0 33.0 41.0 26-27 37.80370575639249 40.0 36.5 41.0 33.0 41.0 28-29 37.52891086198933 39.5 36.0 41.0 33.0 41.0 30-31 37.23223453835496 39.0 35.5 41.0 32.0 41.0 32-33 36.894208338581684 39.0 35.0 41.0 32.0 41.0 34-35 36.72665910903976 39.0 35.0 41.0 31.0 41.0 36-37 36.42670907335096 39.0 35.0 41.0 31.0 41.0 38-39 36.15537053365243 38.0 35.0 41.0 30.0 41.0 40-41 35.88631775622454 38.0 35.0 40.5 30.0 41.0 42-43 35.68049670403494 38.0 35.0 40.0 30.0 41.0 44-45 35.36064785657304 38.0 35.0 40.5 28.0 41.0 46-47 34.83490741907041 38.0 35.0 40.0 24.5 41.0 48-49 34.386783809883696 38.0 34.0 40.0 23.0 41.0 50-51 34.13654574463618 38.0 34.0 40.0 22.5 41.0 52-53 33.77654364529538 38.0 33.0 40.0 21.0 41.0 54-55 33.560177604232265 37.0 33.0 40.0 20.0 41.0 56-57 33.408277700801946 37.0 33.0 40.0 19.5 41.0 58-59 33.19628710584877 37.0 33.0 40.0 18.5 41.0 60-61 32.7535642608221 36.0 32.5 40.0 14.0 41.0 62-63 32.28204097913255 36.0 32.0 39.0 10.0 41.0 64-65 31.77054330940085 35.0 31.0 39.0 7.5 40.0 66-67 31.34855943233825 35.0 31.0 38.0 4.0 40.0 68-69 31.138935214342695 35.0 31.0 37.5 2.0 40.0 70-71 30.696863164966203 35.0 30.5 37.0 2.0 39.0 72-73 30.143853549985305 35.0 30.0 36.5 2.0 39.0 74-75 29.663773775034638 34.0 29.0 36.0 2.0 38.5 76-77 27.64932107318302 32.0 26.5 34.5 2.0 36.0 78-79 28.547288911281857 33.5 28.5 35.0 2.0 37.0 80-81 28.668589662845868 34.0 29.0 35.0 2.0 36.0 82-83 28.394526598648024 34.0 29.0 35.0 2.0 36.0 84-85 28.04292648108494 34.0 28.5 35.0 2.0 36.0 86-87 27.404380064659698 33.0 26.5 35.0 2.0 35.0 88-89 26.668173993366082 33.0 25.0 35.0 2.0 35.0 90-91 26.035368434311625 32.5 23.5 35.0 2.0 35.0 92-93 25.824427509761936 33.0 23.5 35.0 2.0 35.0 94-95 25.565577108787842 33.0 21.5 35.0 2.0 35.0 96-97 24.850806566738044 32.0 16.5 35.0 2.0 35.0 98-99 24.03499055296637 32.0 2.0 34.0 2.0 35.0 100-101 20.612677919133393 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18559604979593303 1101 2 0.22563014578025786 1101 3 0.05912616865943221 1101 4 0.07918313759903839 1101 5 -0.10384832378780118 1101 6 0.10624806479960114 1101 7 0.17882786166717324 1101 8 0.25192416511175963 1101 9 0.34097952751722005 1101 10-11 0.3493151650564741 1101 12-13 0.34671193522440547 1101 14-15 0.33842509834353507 1101 16-17 0.37664046135380147 1101 18-19 0.4264135767114112 1101 20-21 0.40027909496272684 1101 22-23 0.3295773128606001 1101 24-25 0.3218597742649152 1101 26-27 0.31686473879354793 1101 28-29 0.4795186194938452 1101 30-31 0.4876891022559633 1101 32-33 0.3691625844440338 1101 34-35 0.4945260690710782 1101 36-37 0.6124162361212342 1101 38-39 0.6738793555051572 1101 40-41 0.7250739487826863 1101 42-43 0.8012853486717049 1101 44-45 0.6960280625729425 1101 46-47 0.660622715621713 1101 48-49 0.6193031882297575 1101 50-51 0.5529641159298748 1101 52-53 0.5745111437722628 1101 54-55 0.5650918905205913 1101 56-57 0.6175095669532666 1101 58-59 0.7544999100041991 1101 60-61 0.6530618709305998 1101 62-63 0.3438057822109073 1101 64-65 0.5933559396914312 1101 66-67 0.722807460496778 1101 68-69 0.8998324322918165 1101 70-71 0.9873645974594538 1101 72-73 0.7277413341112187 1101 74-75 0.7743052842089639 1101 76-77 0.7889739169445598 1101 78-79 0.6778954462489075 1101 80-81 0.9264270788966122 1101 82-83 0.9615355153692313 1101 84-85 1.0343717683606073 1101 86-87 1.1090813713600518 1101 88-89 1.0676306629730625 1101 90-91 1.0832266471413163 1101 92-93 1.3206596533597157 1101 94-95 1.2477193523001766 1101 96-97 1.2266795339313141 1101 98-99 1.1742376084971475 1101 100-101 1.3578075441066204 1102 1 0.26665067498372963 1102 2 0.3265978474850826 1102 3 0.21647521995502217 1102 4 0.170720351987093 1102 5 -0.040922334913538805 1102 6 0.004354426426814939 1102 7 0.23694072007789657 1102 8 0.16902709272482497 1102 9 0.23643061891884543 1102 10-11 0.2894414317842475 1102 12-13 0.31065442418857714 1102 14-15 0.2446818054024078 1102 16-17 0.3046972850067391 1102 18-19 0.4184252883238315 1102 20-21 0.40788634989768013 1102 22-23 0.4125001258518495 1102 24-25 0.46653719736213617 1102 26-27 0.29625340306645853 1102 28-29 0.3943168566612485 1102 30-31 0.3958622154334108 1102 32-33 0.5743572559214201 1102 34-35 0.4618075857219921 1102 36-37 0.22864847872968852 1102 38-39 0.2639156388809383 1102 40-41 0.4178558482797641 1102 42-43 0.49295460148358927 1102 44-45 0.570778632491681 1102 46-47 0.6503659911607471 1102 48-49 0.7028165849454027 1102 50-51 0.6704768629671705 1102 52-53 0.4786908378433665 1102 54-55 0.5704013433405919 1102 56-57 0.6193440381904978 1102 58-59 0.7594192657785115 1102 60-61 0.6300780490816891 1102 62-63 0.5938278973780058 1102 64-65 0.6263242156655089 1102 66-67 0.8816501919301878 1102 68-69 0.7139765680745853 1102 70-71 0.7924654426213813 1102 72-73 0.8349277482927668 1102 74-75 0.7250490064018607 1102 76-77 0.7652033752172294 1102 78-79 0.6188164088169934 1102 80-81 0.6757737751081727 1102 82-83 0.6865925072424481 1102 84-85 0.9290303391842443 1102 86-87 0.8965314128350528 1102 88-89 0.7858421042728843 1102 90-91 0.8152738445333227 1102 92-93 0.756160587272344 1102 94-95 0.8824866427354046 1102 96-97 1.054502444374318 1102 98-99 1.2617139706464258 1102 100-101 1.4509163855328993 1103 1 0.1511617404893073 1103 2 0.11766640370007053 1103 3 0.18244809698646947 1103 4 0.15252328060210374 1103 5 0.02648018325163548 1103 6 0.056112704756174026 1103 7 0.14901293706164864 1103 8 0.003775276206994249 1103 9 0.10643450155237844 1103 10-11 0.21532265093117786 1103 12-13 0.16550840943456535 1103 14-15 0.15134537520661695 1103 16-17 0.10214029349306486 1103 18-19 0.13056545139443898 1103 20-21 0.1836815964449059 1103 22-23 0.27435255804443415 1103 24-25 0.2686989922481544 1103 26-27 0.3221034224796213 1103 28-29 0.08688092011457371 1103 30-31 0.31575863231731915 1103 32-33 0.40573586815725804 1103 34-35 0.38430120710975046 1103 36-37 0.3546024972553994 1103 38-39 0.2257951108427747 1103 40-41 0.3030713344409506 1103 42-43 0.20414005270107083 1103 44-45 0.38281063648523883 1103 46-47 0.15558189331837724 1103 48-49 -0.02879498530253244 1103 50-51 0.08622232860586365 1103 52-53 0.191428649147376 1103 54-55 0.10158374391744474 1103 56-57 0.020600347469986957 1103 58-59 0.08780062359091545 1103 60-61 0.11870470613420991 1103 62-63 0.0191752544138879 1103 64-65 0.2563619326769988 1103 66-67 -0.02702610736538702 1103 68-69 0.024126881458577998 1103 70-71 0.006503936159571566 1103 72-73 0.27460340972712416 1103 74-75 0.26619868192462803 1103 76-77 0.2720381968934156 1103 78-79 0.2584532439159979 1103 80-81 0.3689944196253414 1103 82-83 0.20342927023540724 1103 84-85 0.22679089162753385 1103 86-87 0.1280292773866094 1103 88-89 -0.2079720285131792 1103 90-91 -0.283448353037258 1103 92-93 -0.09683250718657987 1103 94-95 -0.18675388641208102 1103 96-97 0.1081562798615856 1103 98-99 0.09865098878387002 1103 100-101 0.7509656117794989 1104 1 0.12201471381604989 1104 2 0.1574054499796702 1104 3 0.11453120113819182 1104 4 0.04833395686770814 1104 5 -0.07844855577375398 1104 6 0.05428714271003798 1104 7 0.0710679824221998 1104 8 -0.08961872352843159 1104 9 0.06636961124623753 1104 10-11 0.057316496099566905 1104 12-13 -0.03123767591150539 1104 14-15 0.08087881947544417 1104 16-17 0.2236090981050154 1104 18-19 0.14411339154496972 1104 20-21 0.14494236217836942 1104 22-23 0.011315663867151216 1104 24-25 0.14202260131887812 1104 26-27 0.06919206180231896 1104 28-29 0.15260045752745555 1104 30-31 -0.14033423323024863 1104 32-33 -0.0963504387348948 1104 34-35 0.14466416115042335 1104 36-37 0.20578402997556822 1104 38-39 0.08120147594125626 1104 40-41 0.30086840602220377 1104 42-43 0.3577083232908649 1104 44-45 0.2648349076877352 1104 46-47 0.4237884283013571 1104 48-49 0.5340086658215668 1104 50-51 0.38470694500806957 1104 52-53 0.5587683554700078 1104 54-55 0.46922875495297944 1104 56-57 0.22921014924821037 1104 58-59 0.2619837507188265 1104 60-61 0.4082213012033833 1104 62-63 0.505095728976471 1104 64-65 0.43236052625619337 1104 66-67 0.39798416229143285 1104 68-69 0.457872973767131 1104 70-71 0.20707590382581387 1104 72-73 0.3286075315148089 1104 74-75 0.31236270400961175 1104 76-77 0.3939195283979373 1104 78-79 0.26634973970021747 1104 80-81 0.3227837489297194 1104 82-83 0.39403131581179096 1104 84-85 0.5045965966829904 1104 86-87 0.667192178512444 1104 88-89 0.5116249135659565 1104 90-91 0.6917958559206667 1104 92-93 0.5826891008676149 1104 94-95 0.37906556257731694 1104 96-97 0.65243347008348 1104 98-99 0.996855365398293 1104 100-101 1.5373013982263402 1105 1 0.16332637835927244 1105 2 0.21799616797967758 1105 3 0.09548939215942909 1105 4 0.14068569290244426 1105 5 0.0748632526378401 1105 6 0.034564033991202336 1105 7 0.0377887380631563 1105 8 0.05249625220069731 1105 9 0.14339659901604307 1105 10-11 0.14869262474001488 1105 12-13 0.17567478433448258 1105 14-15 0.24735631306707972 1105 16-17 0.14074714119082188 1105 18-19 0.21823050316607606 1105 20-21 0.08005011820527841 1105 22-23 0.10771438952537693 1105 24-25 0.19915944089041915 1105 26-27 0.1250821574332761 1105 28-29 0.20315946915174266 1105 30-31 0.18668237941661658 1105 32-33 0.23911601987597209 1105 34-35 0.28490781053766767 1105 36-37 0.4491152467838191 1105 38-39 0.5032300061460049 1105 40-41 0.2569307978615498 1105 42-43 0.3509136552740486 1105 44-45 0.4622458303404997 1105 46-47 0.6599943565082711 1105 48-49 0.7358625619568997 1105 50-51 0.5257200826804151 1105 52-53 0.45400243899389636 1105 54-55 0.44131898255550084 1105 56-57 0.641398567014285 1105 58-59 0.6631721173009666 1105 60-61 0.7193954469503439 1105 62-63 0.708926777707731 1105 64-65 0.8083615527016939 1105 66-67 0.6983827053475018 1105 68-69 0.6931012195551745 1105 70-71 0.6128746292073792 1105 72-73 0.5192552573570275 1105 74-75 0.571818245224442 1105 76-77 0.8022414836797758 1105 78-79 0.5171458574675469 1105 80-81 0.8035206039947873 1105 82-83 0.9585175466402944 1105 84-85 0.8255614001159088 1105 86-87 0.6980695819096709 1105 88-89 0.8001920122527402 1105 90-91 0.8595723149050372 1105 92-93 1.005286533647194 1105 94-95 1.1623029606071498 1105 96-97 1.3445931971762484 1105 98-99 1.3582765774063255 1105 100-101 1.8320306282677166 1106 1 0.1470081469833815 1106 2 0.08027204596498905 1106 3 0.009423089977026677 1106 4 0.05966089258623697 1106 5 -0.1110343363013584 1106 6 -0.0063767810847608075 1106 7 -0.02073565771264896 1106 8 0.015647263816980228 1106 9 0.011983307277418476 1106 10-11 0.03960067867520678 1106 12-13 0.03108939182418169 1106 14-15 -0.058230854115535635 1106 16-17 0.11391815434568286 1106 18-19 0.011394300911788946 1106 20-21 0.008575787288577885 1106 22-23 0.039798024606064075 1106 24-25 -0.070515149462679 1106 26-27 0.11499384825300041 1106 28-29 0.2843328220091266 1106 30-31 0.2998038131608567 1106 32-33 0.2275852920480972 1106 34-35 0.1268585176212369 1106 36-37 -0.034607639061505324 1106 38-39 0.009886353716517249 1106 40-41 0.29099774200313533 1106 42-43 0.11827241761915985 1106 44-45 0.10626155038261942 1106 46-47 0.0017534042864824073 1106 48-49 -0.06636758306752455 1106 50-51 0.10264310858957515 1106 52-53 0.2793543891439114 1106 54-55 0.17714216258791993 1106 56-57 -0.10988203547237418 1106 58-59 -0.0813765349826312 1106 60-61 0.04539665128702097 1106 62-63 0.1863083269515755 1106 64-65 0.08808598773780929 1106 66-67 0.019555288914940405 1106 68-69 -0.10261980407794269 1106 70-71 0.02161561989366234 1106 72-73 0.19358630669503896 1106 74-75 0.15784343018562197 1106 76-77 0.014773747434812634 1106 78-79 0.1791370981730651 1106 80-81 0.18190647452594177 1106 82-83 0.21145941750038943 1106 84-85 0.3249804535709515 1106 86-87 0.37284496250924093 1106 88-89 0.4554393654974227 1106 90-91 0.3126317014360964 1106 92-93 0.4359489718718841 1106 94-95 0.6406123000523571 1106 96-97 0.7106850185630371 1106 98-99 1.114429570607392 1106 100-101 1.5478801233217112 1107 1 0.10452445262614418 1107 2 0.006325203276801972 1107 3 -0.01507792200705893 1107 4 0.025898851546557466 1107 5 -0.1981618462968271 1107 6 -0.07171455859488418 1107 7 -0.029603937444427686 1107 8 0.033780500097591926 1107 9 -0.02398729412652756 1107 10-11 -0.08205669728801723 1107 12-13 0.01084709167467679 1107 14-15 0.014654910816133793 1107 16-17 -0.10485369233545327 1107 18-19 0.12394316547349149 1107 20-21 -0.09360318595996375 1107 22-23 0.08562008821574096 1107 24-25 -0.05593433081599386 1107 26-27 -0.023452252138959295 1107 28-29 0.10582890891669905 1107 30-31 -0.025525507147172277 1107 32-33 -0.21365548161570302 1107 34-35 -0.03278426377710275 1107 36-37 -0.14957125738247612 1107 38-39 -0.07586849739221435 1107 40-41 -0.00819083789385644 1107 42-43 0.0852670736100336 1107 44-45 0.1395012348216369 1107 46-47 -0.3005581873170584 1107 48-49 -0.14949297745329915 1107 50-51 -0.19341722294321784 1107 52-53 -0.02255463440272365 1107 54-55 0.06259878604343783 1107 56-57 -0.09820297974648184 1107 58-59 -0.10473791934544607 1107 60-61 0.011268698793976739 1107 62-63 0.18112992711705544 1107 64-65 0.10239646393327462 1107 66-67 -0.14522399905672145 1107 68-69 -0.19229756634939577 1107 70-71 -0.21822984633906017 1107 72-73 -0.3364518161726586 1107 74-75 -0.16635084457657 1107 76-77 -0.22766779182834185 1107 78-79 -0.12545047576199408 1107 80-81 -7.31125199660454E-4 1107 82-83 -0.062297989097277906 1107 84-85 -0.1950831456362181 1107 86-87 -0.22535218717799665 1107 88-89 -0.1355802902970744 1107 90-91 0.10358367343045316 1107 92-93 0.09534170286739752 1107 94-95 0.156806663837731 1107 96-97 0.2414143802406521 1107 98-99 0.45089726173885225 1107 100-101 1.1875227999869011 1108 1 0.23324561818112954 1108 2 0.07937390336978467 1108 3 -0.03370210191845047 1108 4 0.09736423006116723 1108 5 0.03956093424616114 1108 6 0.09615351329367172 1108 7 0.103228106681307 1108 8 0.04987424425009124 1108 9 0.08404705531542334 1108 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2316 66-67 -1.4282216432254167 2316 68-69 -1.355281789274823 2316 70-71 -1.3717566574066034 2316 72-73 -1.4956696137845817 2316 74-75 -1.641042849570411 2316 76-77 -1.5676514776669102 2316 78-79 -1.3715070963356233 2316 80-81 -1.467993132292051 2316 82-83 -1.5621604569224559 2316 84-85 -2.062849812816655 2316 86-87 -2.1379247027956083 2316 88-89 -2.3397224985658376 2316 90-91 -2.6767575842064346 2316 92-93 -2.5694627465355815 2316 94-95 -2.349679578795108 2316 96-97 -2.38737353447517 2316 98-99 -2.390041146373651 2316 100-101 -2.3284291538591972 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 32.0 5 118.0 6 317.0 7 651.0 8 1049.0 9 1590.0 10 2576.0 11 3989.0 12 5770.0 13 8083.0 14 8566.0 15 11076.0 16 14111.0 17 18878.0 18 17291.0 19 10810.0 20 9155.0 21 9663.0 22 10481.0 23 11202.0 24 12863.0 25 14996.0 26 17230.0 27 20513.0 28 24588.0 29 28939.0 30 34127.0 31 41326.0 32 49927.0 33 62832.0 34 87830.0 35 143571.0 36 157026.0 37 198461.0 38 138323.0 39 12883.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.94956705748073 17.824673220356672 13.352491050332777 16.873268671829823 2 13.689213586933702 25.70970315320989 39.10274173909393 21.49834152076248 3 24.151152538103034 32.19784187765042 29.083091909140528 14.567913675106018 4 10.89801402359659 17.25725322248814 38.96334550951001 32.88138724440526 5 10.501276310517655 44.01578778051323 31.606639334554625 13.87629657441449 6 23.68269452016372 36.845924007517624 20.448907542983637 19.02247392933502 7 24.29012889952555 38.96435319309737 20.570852752235798 16.174665155141284 8 23.556871142461265 30.915060670949323 21.21316706554142 24.314901121047992 9 27.482806398790782 20.864760465213923 22.516773733047824 29.13565940294747 10-11 22.91212159381954 27.361884368308353 26.467397237267498 23.25859680060461 12-13 31.54838980560104 25.40151992274426 26.029894613091486 17.02019565856321 14-15 26.494352773229206 25.859764034093295 31.10849393290507 16.537389259772432 16-17 17.485191808436355 30.122928879112166 33.056015209808784 19.335864102642695 18-19 16.66171222236218 31.62371415375572 35.19343326195575 16.521140361926356 20-21 19.294285594323384 28.888357055884452 32.29063274131923 19.52672460847294 22-23 17.822353780912795 28.38094638283579 30.998068606457572 22.798631229793845 24-25 19.360788685321527 28.777525155509647 33.06801697117797 18.793669187990854 26-27 21.648400649296665 25.360484772347284 35.59732018168886 17.39379439666719 28-29 17.87429147247764 27.322374774320863 37.24692446571776 17.556409287483728 30-31 17.338959566696058 31.04862073308981 34.62614938909183 16.986270311122308 32-33 16.958307091573246 30.586681781920475 33.44602594785238 19.0089851786539 34-35 18.818267821278923 27.375320226023618 33.88956049516255 19.91685145753491 36-37 20.792948211856007 27.304513614755336 35.141228939544106 16.76130923384455 38-39 22.074396271997696 25.58625876558846 35.8607315494835 16.478613412930343 40-41 18.072092356285374 28.29887216861161 36.972999483932846 16.65603599117017 42-43 16.60787934826699 31.225975932471844 33.93586135598829 18.230283363272875 44-45 17.50178032173437 31.196107477687196 31.355958535299077 19.946153665279358 46-47 17.851871974565885 30.05723263759283 30.013650011357512 22.077245376483773 48-49 19.583700200995192 29.297306194153716 30.889480355837488 20.229513249013603 50-51 20.72265249414493 30.509619607523707 31.668498692949314 17.099229205382045 52-53 18.01402359659067 31.13137674770122 32.65255909644372 18.20204055926439 54-55 15.790611747911157 30.134231851198724 33.78519544862913 20.28996095226099 56-57 18.98955062260856 31.73319763040518 29.68518348236723 19.59206826461903 58-59 18.885403029618512 29.686826894897145 30.59362149334547 20.83414858213887 60-61 18.487335327769905 28.38776045767071 32.643636172781186 20.481268041778197 62-63 22.164634197545585 27.7408941968402 31.338666850291474 18.755804755322742 64-65 20.169689223596436 29.648377929904 32.57828192915158 17.603650917347988 66-67 17.757913888528478 29.588279185619733 33.02899257245624 19.624814353395553 68-69 17.191104975414525 32.341978871314545 31.43837718662916 19.02853896664177 70-71 17.918056781338358 31.90715195629337 29.060359457832185 21.11443180453609 72-73 18.00482848385607 29.717008859218208 29.01079061174791 23.267372045177815 74-75 18.136525687001377 31.4097420698151 29.90518081480763 20.548551428375887 76-77 19.773845628383736 28.282613983576166 31.68383591960916 20.25970446843094 78-79 17.991780635297857 28.21431064220199 33.62799932388283 20.165909398617323 80-81 17.378248092617483 31.876706525303494 31.432563830291976 19.312481551787048 82-83 16.988044938234488 31.001369207895806 30.13244073798668 21.878145115883022 84-85 17.624301969181676 28.870176764495948 30.201242809757733 23.30427845656464 86-87 19.109480681709286 29.081084534439366 30.915317611495645 20.894117172355703 88-89 20.19377475223015 26.88053745231792 31.610422914197322 21.315264881254606 90-91 17.791163050465244 27.86435819888932 32.976132016818234 21.368346733827202 92-93 16.628542541569153 30.92257023782232 31.62606111448406 20.822826106124463 94-95 16.33811900269304 31.245029798118484 30.075610048981943 22.341241150206535 96-97 15.901792837049166 29.400596212789182 29.42121173951379 25.276399210647853 98-99 17.988285678297014 28.909182516689757 29.211067724734434 23.891464080278794 100-101 19.286373488138135 28.6011556616251 30.282835628505424 21.82963522173134 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14924.0 1 9634.5 2 9311.5 3 11806.5 4 7130.0 5 5598.0 6 8747.0 7 15221.5 8 19526.5 9 18044.5 10 12482.5 11 7601.0 12 6402.0 13 6137.0 14 5817.5 15 5365.0 16 4673.5 17 4312.5 18 4385.5 19 4464.0 20 4546.5 21 4801.0 22 5113.5 23 5565.5 24 5995.5 25 6517.5 26 7497.0 27 8512.0 28 9419.0 29 10427.0 30 11722.0 31 12973.5 32 14442.0 33 16167.0 34 17766.0 35 19017.5 36 20522.5 37 22271.5 38 22791.0 39 22555.5 40 22043.5 41 21702.5 42 21808.5 43 22581.0 44 25886.0 45 28197.5 46 33151.0 47 53913.5 48 135754.0 49 134585.5 50 62711.0 51 45079.0 52 25674.0 53 17469.5 54 14526.0 55 13193.5 56 12458.0 57 11452.5 58 10697.0 59 10315.0 60 10056.0 61 9646.5 62 8092.5 63 5083.5 64 2766.0 65 2019.5 66 1476.0 67 1052.5 68 806.0 69 644.0 70 521.0 71 445.5 72 389.0 73 455.5 74 439.0 75 308.0 76 227.5 77 155.0 78 107.5 79 77.5 80 43.5 81 31.5 82 25.0 83 18.5 84 10.5 85 4.0 86 3.0 87 1.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23655372213125078 2 0.0 3 0.0 4 0.0 5 0.02594785237435445 6 0.004030734349414283 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0033169584750388378 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.298022420959819E-4 26-27 0.0019313935424276779 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007515640089012051 36-37 0.019313935424276778 38-39 0.015577108787840618 40-41 0.009531007263719192 42-43 0.009824914976697317 44-45 0.00453457614309107 46-47 7.137758743754461E-4 48-49 0.0021413276231263384 50-51 8.397363227946425E-5 52-53 0.0 54-55 0.0 56-57 0.0011336440357727674 58-59 5.878154259562498E-4 60-61 0.0031490112104799097 62-63 0.001343578116471428 64-65 0.00218331443926607 66-67 0.0066759037662174085 68-69 3.778813452575891E-4 70-71 8.397363227946425E-5 72-73 0.0 74-75 3.778813452575891E-4 76-77 0.05789981945669061 78-79 0.14334299030104547 80-81 0.0013855649326111601 82-83 0.0013015913003316959 84-85 0.0 86-87 0.10513498761388923 88-89 0.35390687324180203 90-91 0.3894277196960155 92-93 2.519208968383927E-4 94-95 2.519208968383927E-4 96-97 0.0 98-99 0.0 100-101 0.0014275517487508922 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1190850.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.43917615854996 #Duplication Level Percentage of deduplicated Percentage of total 1 76.36738256178369 27.063971233744283 2 12.993667667436856 9.209697548238992 3 4.476281180032147 4.759071518230835 4 2.071689929397049 2.9367593741518383 5 1.1005401782275361 1.9501118622883815 6 0.6808915325935627 1.4478140981068996 7 0.4263752585083923 1.0577271527148335 8 0.3124963312969044 0.8859690026985266 9 0.2180818658162308 0.6955777493681993 >10 1.1440795469248397 7.47066923944956 >50 0.10768942630809193 2.6646963913492137 >100 0.07194989340777307 5.097509104976117 >500 0.00994044579681437 2.466220097250245 >1k 0.013963959571715426 10.311774266433533 >5k 0.0023667728087653265 5.4632105504301425 >10k+ 0.002603450089641859 16.51922081056845 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 32351 2.716630977872948 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 30364 2.549775370533652 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21464 1.8024100432464205 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 17186 1.4431708443548725 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16525 1.3876642734181468 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16457 1.3819540664231431 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13896 1.1668975941554351 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13351 1.1211319645631272 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13087 1.0989629256413487 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 11264 0.9458789939958853 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 11002 0.9238779023386656 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 9023 0.757694084057606 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 7998 0.6716211109711551 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7212 0.6056178359994961 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7081 0.5946172901708864 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6314 0.5302095142125373 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5820 0.4887265398664819 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5705 0.47906957215434354 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5441 0.45690053323256496 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5365 0.45051853717932566 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5175 0.43456354704622746 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4881 0.409875299156065 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4775 0.4009740941344418 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4700 0.394676071713482 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4419 0.3710794810429525 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4107 0.3448797077717597 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4101 0.3443758659780829 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3995 0.33547466095645967 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3651 0.306587731452324 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3621 0.30406852248394006 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3397 0.28525842885334 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3246 0.272578410379141 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3017 0.2533484485871436 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2537 0.2130411050930008 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2520 0.21161355334424992 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2489 0.2090103707435865 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2450 0.2057353990846874 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2254 0.1892765671579124 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2233 0.18751312088004368 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2205 0.18516185917621866 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2191 0.18398622832430617 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2189 0.18381828105974723 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2145 0.18012344123945082 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2066 0.17348952428937314 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1985 0.16668766007473654 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1926 0.16173321577024816 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1900 0.15954990133098207 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1898 0.15938195406642314 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1852 0.15551916698156779 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1780 0.14947306545744635 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1765 0.14821346097325439 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1732 0.1454423311080321 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1630 0.13687702061552673 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1587 0.13326615442750975 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1541 0.12940336734265442 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1520 0.12763992106478564 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1448 0.12159381954066423 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1434 0.12041818868875173 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1408 0.11823487424948566 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1380 0.11588361254566065 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1372 0.11521182348742495 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1362 0.1143720871646303 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1311 0.11008943191837763 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1300 0.10916572196330353 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1299 0.10908174833102406 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1254 0.10530293487844816 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1245 0.10454717218793298 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1209 0.10152412142587228 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.397363227946425E-5 0.0 12-13 0.0 0.0 0.0 1.679472645589285E-4 0.0 14-15 0.0 0.0 0.0 1.679472645589285E-4 0.0 16-17 0.0 0.0 0.0 6.298022420959819E-4 0.0 18-19 0.0 0.0 0.0 0.0010496704034933032 0.0 20-21 0.0 8.397363227946425E-5 0.0 0.0017634462778687494 0.0 22-23 0.0 8.397363227946425E-5 0.0 0.004324642062392409 0.0 24-25 0.0 8.397363227946425E-5 0.0 0.007599613721291514 0.0 26-27 0.0 8.397363227946425E-5 0.0 0.01226015031280178 0.0 28-29 0.0 8.397363227946425E-5 0.0 0.02309274887685267 0.0 30-31 0.0 8.397363227946425E-5 0.0 0.04853675945753033 0.0 32-33 0.0 8.397363227946425E-5 0.0 0.09421841541755889 0.0 34-35 0.0 8.397363227946425E-5 0.0 0.17126422303396732 0.0 36-37 0.0 8.397363227946425E-5 0.0 0.2764831842801361 0.0 38-39 0.0 8.397363227946425E-5 0.0 0.40475290758701765 0.0 40-41 0.0 8.397363227946425E-5 0.0 0.5498593441659319 0.0 42-43 0.0 8.397363227946425E-5 0.0 0.6845950371583323 0.0 44-45 0.0 8.397363227946425E-5 0.0 0.8305412100600411 0.0 46-47 0.0 8.397363227946425E-5 0.0 1.0212873157828442 0.0 48-49 0.0 8.397363227946425E-5 0.0 1.2430616786329094 0.0 50-51 0.0 8.397363227946425E-5 0.0 1.4591678213041104 0.0 52-53 0.0 8.397363227946425E-5 0.0 1.668346139312256 0.0 54-55 0.0 8.397363227946425E-5 0.0 1.888566989965151 0.0 56-57 0.0 8.397363227946425E-5 0.0 2.149557039089726 0.0 58-59 0.0 8.397363227946425E-5 0.0 2.498719402107738 0.0 60-61 0.0 1.679472645589285E-4 0.0 2.9759415543519334 0.0 62-63 0.0 1.679472645589285E-4 0.0 3.383801486333291 0.0 64-65 0.0 2.5192089683839276E-4 0.0 3.708191627828862 0.0 66-67 0.0 2.5192089683839276E-4 0.0 4.068228576227065 0.0 68-69 0.0 2.5192089683839276E-4 0.0 4.463912331527901 0.0 70-71 0.0 2.5192089683839276E-4 0.0 4.944703363143972 0.0 72-73 0.0 2.5192089683839276E-4 0.0 5.44514422471344 0.0 74-75 0.0 2.5192089683839276E-4 0.0 5.924423730948482 0.0 76-77 0.0 2.5192089683839276E-4 0.0 6.359617080236806 0.0 78-79 0.0 2.5192089683839276E-4 0.0 6.872360078935214 0.0 80-81 0.0 2.5192089683839276E-4 0.0 7.424486711172692 0.0 82-83 0.0 2.5192089683839276E-4 0.0 8.00638199605324 0.0 84-85 0.0 2.5192089683839276E-4 0.0 8.748037116345468 0.0 86-87 0.0 2.5192089683839276E-4 0.0 9.528823949279927 0.0 88-89 0.0 2.5192089683839276E-4 0.0 10.156988705546457 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 925 0.0 50.28332 2 ATCACGC 70 7.972267E-8 47.461296 3 TATAACG 70 7.972267E-8 47.461296 2 TATCACG 75 1.371609E-7 44.29721 2 ACCTGGG 1115 0.0 41.714863 3 TCACGCA 95 8.755924E-7 34.971485 4 AGTACTC 615 0.0 31.640867 5 ATAACGC 95 3.5104236E-5 29.975557 3 GTCACTA 270 0.0 29.964842 1 CGACCGT 40 4.5243613E-4 29.654593 18-19 GTAAGCG 85 3.6870915E-9 27.911377 94-95 GCTTATG 445 0.0 27.806107 1 AAGTACT 700 0.0 27.798761 4 GTCCTAC 120 5.302174E-6 27.761545 1 CCTGGGA 1275 0.0 27.546165 4 ACACGGG 140 5.847887E-7 27.12074 3 ATGCCGC 35 0.008437145 27.113909 94-95 TCGACCG 45 9.0085185E-4 26.359638 18-19 GTACTTG 275 0.0 25.958845 1 ATGGGAT 3600 0.0 24.389833 5 >>END_MODULE