FastQCFastQC Report
Thu 26 May 2016
SRR938705_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938705_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4469573
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154630.34596145985310006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118430.26496938298132733No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78460.17554249589390306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75950.16992674691743484No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG207050.028.7539621
TACATGG208500.027.9923762
ACATGGG209450.026.508333
GTATCAA264450.024.1259691
GGTATCA196500.022.9404131
CATGGGA115550.022.098054
CATGGGG97650.022.0251434
TACCTGG31350.021.760062
GAGTACT139300.021.42874712-13
ATCAACG305450.020.6430113
TCAACGC306050.020.6180214
CAACGCA308200.020.4127065
TATCAAC312250.020.2389762
AACGCAG314150.020.1478166
GTACTTT152600.019.608314-15
AGTACTT145100.019.2833712-13
ATGGGAG54400.019.1583865
GTACCTG38800.018.324951
ACGCAGA347100.018.0859787
CGCAGAG349000.017.9330548
Produced by FastQC (version 0.11.5)