##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938705_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4469573 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.425778704140193 33.0 31.0 34.0 28.0 34.0 2 31.476717350852084 33.0 31.0 34.0 28.0 34.0 3 31.494642776837967 33.0 31.0 34.0 28.0 34.0 4 35.046844519599524 37.0 35.0 37.0 32.0 37.0 5 35.07792511723156 37.0 35.0 37.0 32.0 37.0 6 35.16094423337531 37.0 35.0 37.0 32.0 37.0 7 35.06028786195012 37.0 35.0 37.0 32.0 37.0 8 35.0231339772278 37.0 35.0 37.0 32.0 37.0 9 36.56616325541612 39.0 37.0 39.0 32.0 39.0 10-11 36.449375365387255 39.0 37.0 39.0 32.0 39.0 12-13 36.3175531532878 39.0 37.0 39.0 32.0 39.0 14-15 37.43867501436938 40.0 37.0 41.0 32.0 41.0 16-17 37.368254305277034 40.0 37.0 41.0 32.0 41.0 18-19 37.289392633256014 40.0 37.0 41.0 31.0 41.0 20-21 37.17151325193704 40.0 37.0 41.0 31.0 41.0 22-23 37.084327742269785 40.0 36.5 41.0 31.0 41.0 24-25 37.09250212492334 40.0 37.0 41.0 31.0 41.0 26-27 36.99497245038844 40.0 36.0 41.0 30.0 41.0 28-29 36.868306323668946 40.0 36.0 41.0 30.0 41.0 30-31 36.66463138648815 39.0 36.0 41.0 30.0 41.0 32-33 36.45444687893004 39.0 35.5 41.0 30.0 41.0 34-35 36.11275719179439 39.0 35.0 41.0 28.5 41.0 36-37 35.85445153261844 39.0 35.0 41.0 27.0 41.0 38-39 35.67590147873186 39.0 35.0 41.0 27.0 41.0 40-41 35.495099308144205 39.0 35.0 40.0 26.5 41.0 42-43 35.22531861544716 38.0 34.5 40.0 25.5 41.0 44-45 35.18700432904888 39.0 35.0 40.5 25.0 41.0 46-47 34.997806949343925 39.0 34.5 41.0 24.0 41.0 48-49 34.759912412214774 39.0 34.0 41.0 23.5 41.0 50-51 33.616568405975244 37.5 32.5 39.5 22.0 40.5 52-53 33.70912825453349 37.5 33.0 39.5 22.0 40.5 54-55 34.22377182339342 38.0 34.0 40.0 22.5 41.0 56-57 34.12557989320233 38.0 34.0 40.0 22.0 41.0 58-59 33.894358812351875 38.0 33.5 40.0 21.0 41.0 60-61 33.59626120884478 37.5 33.0 40.0 20.0 41.0 62-63 33.23207116205508 37.0 33.0 40.0 18.5 41.0 64-65 32.832055545350755 36.0 32.0 39.5 17.5 41.0 66-67 32.4313855037159 36.0 32.0 39.0 13.5 41.0 68-69 32.02914282863262 35.0 31.0 39.0 12.0 40.5 70-71 31.621487444102602 35.0 31.0 38.5 8.0 40.0 72-73 31.217438891813604 35.0 31.0 37.5 7.0 40.0 74-75 30.76591913813691 35.0 30.5 37.0 4.5 39.0 76-77 30.332275029404375 35.0 30.0 36.5 2.0 39.0 78-79 29.92249852502689 34.0 30.0 36.0 2.0 38.5 80-81 29.49155769466121 34.0 29.0 35.5 2.0 37.0 82-83 29.04311921966595 34.0 29.0 35.0 2.0 37.0 84-85 28.65128257218307 34.0 29.0 35.0 2.0 36.5 86-87 28.275575653423715 34.0 28.0 35.0 2.0 36.0 88-89 27.90248263089114 34.0 27.0 35.0 2.0 36.0 90-91 27.497315291639715 34.0 26.5 35.0 2.0 35.0 92-93 27.058921735924216 33.5 25.5 35.0 2.0 35.0 94-95 26.55176948670488 33.0 25.0 35.0 2.0 35.0 96-97 25.96779435082501 33.0 23.5 35.0 2.0 35.0 98-99 25.262751945208187 33.0 19.0 35.0 2.0 35.0 100-101 22.999174529647462 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38928895558790444 1101 2 0.1908024929846448 1101 3 0.3754662188671354 1101 4 0.3932644688850786 1101 5 0.158629382323781 1101 6 0.07378965863305353 1101 7 0.2624633819052775 1101 8 0.3226555081039564 1101 9 0.46713852313781246 1101 10-11 0.4494576247247295 1101 12-13 0.4597625249492907 1101 14-15 0.6649463907794626 1101 16-17 0.6210018729068096 1101 18-19 0.5608693787749317 1101 20-21 0.6584542882674072 1101 22-23 0.5980589867205168 1101 24-25 0.6569608522178569 1101 26-27 0.6215904188142432 1101 28-29 0.48235268067418957 1101 30-31 0.47765681318902864 1101 32-33 0.5172722754720738 1101 34-35 0.4317886794984389 1101 36-37 0.5975295679721313 1101 38-39 0.6067108249516622 1101 40-41 0.560456222379706 1101 42-43 0.5789115262233295 1101 44-45 0.7196260873908926 1101 46-47 0.707347685510733 1101 48-49 0.5567961360880034 1101 50-51 0.42865746577526664 1101 52-53 0.5117017212487198 1101 54-55 0.5374691422063265 1101 56-57 0.5796320905083334 1101 58-59 0.4383439545825709 1101 60-61 0.3857247833078006 1101 62-63 0.41079812602927035 1101 64-65 0.38735661750765615 1101 66-67 0.3121725239203741 1101 68-69 0.325038376940455 1101 70-71 0.13085765150020023 1101 72-73 0.1430952537124348 1101 74-75 0.16613244497012047 1101 76-77 0.1327539218295044 1101 78-79 0.047540039292396585 1101 80-81 0.00636792217862947 1101 82-83 0.027670725816552277 1101 84-85 0.07002877929326701 1101 86-87 0.029592846933230277 1101 88-89 -0.12925912100623194 1101 90-91 -0.18642987884964768 1101 92-93 -0.23181305896136806 1101 94-95 -0.3384006129483481 1101 96-97 -0.4153441432189062 1101 98-99 -0.6038493501519753 1101 100-101 -0.4497914338450286 1102 1 0.26695157436376604 1102 2 0.32107581927862583 1102 3 0.3651811673569192 1102 4 0.3316441305424078 1102 5 0.3161987692722761 1102 6 0.31771415499490985 1102 7 0.37927439748736447 1102 8 0.3511066239255527 1102 9 0.39054098881042165 1102 10-11 0.3699976495577033 1102 12-13 0.3620902072771983 1102 14-15 0.3567734876380868 1102 16-17 0.4538269640252466 1102 18-19 0.5494650493100721 1102 20-21 0.49443716691880724 1102 22-23 0.5160565867977738 1102 24-25 0.4707790881479852 1102 26-27 0.5535661177495257 1102 28-29 0.5032563877316321 1102 30-31 0.5159133389684172 1102 32-33 0.5488933156391127 1102 34-35 0.5398848041086737 1102 36-37 0.6017507249691931 1102 38-39 0.5762205927111239 1102 40-41 0.5110414014319957 1102 42-43 0.49003692007609345 1102 44-45 0.6267146191057122 1102 46-47 0.6102370647972108 1102 48-49 0.6137816368185867 1102 50-51 0.6144730938737837 1102 52-53 0.5884519155696424 1102 54-55 0.5773678050989659 1102 56-57 0.5290912108216048 1102 58-59 0.5023378312968276 1102 60-61 0.4411871668405709 1102 62-63 0.3599540107211112 1102 64-65 0.3902588196003691 1102 66-67 0.3627749583538815 1102 68-69 0.21106167645429963 1102 70-71 0.19425262928719533 1102 72-73 0.17623065146480243 1102 74-75 0.17104144262244247 1102 76-77 0.19175972691968113 1102 78-79 0.044428160571872155 1102 80-81 -0.01064345634691577 1102 82-83 -0.13886433558780098 1102 84-85 -0.14325343167514148 1102 86-87 -0.14587659705828315 1102 88-89 -0.04743940649571954 1102 90-91 0.037804750009264865 1102 92-93 -0.08340915534032334 1102 94-95 -0.15897628307189748 1102 96-97 -0.17779115679789115 1102 98-99 -0.07557888814573488 1102 100-101 0.007711200634812343 1103 1 0.10372017303203407 1103 2 0.17962312341216347 1103 3 0.17760925142623663 1103 4 0.29816818383183374 1103 5 0.14417766887637384 1103 6 0.24060902975658394 1103 7 0.1777151780752959 1103 8 0.11231555142253313 1103 9 0.373739528734518 1103 10-11 0.22967491928922357 1103 12-13 0.26551950143797853 1103 14-15 0.34125762720547215 1103 16-17 0.4180894093056722 1103 18-19 0.47239108699677956 1103 20-21 0.3414285722459951 1103 22-23 0.5127352329606083 1103 24-25 0.4157954605503065 1103 26-27 0.27419276496035394 1103 28-29 0.36757450836373096 1103 30-31 0.37755100949642895 1103 32-33 0.3058692963502949 1103 34-35 0.28762791697325696 1103 36-37 0.3938258847849454 1103 38-39 0.28850963544488195 1103 40-41 0.43471988961324115 1103 42-43 0.3510943875023145 1103 44-45 0.41904462870326853 1103 46-47 0.3774059790984481 1103 48-49 0.45947335727311156 1103 50-51 0.42204205758166324 1103 52-53 0.3477442199884919 1103 54-55 0.3779928797171479 1103 56-57 0.37517300957865984 1103 58-59 0.3676709655451589 1103 60-61 0.37962011335807944 1103 62-63 0.2244492028080387 1103 64-65 0.07484593096540948 1103 66-67 -0.038959464801287425 1103 68-69 0.0742731004936843 1103 70-71 0.00225704843761676 1103 72-73 0.017376093273046678 1103 74-75 0.11199696084898747 1103 76-77 -0.07977135195832474 1103 78-79 -0.05130295209005098 1103 80-81 -0.08854777646734036 1103 82-83 -0.022905285735067338 1103 84-85 0.02105773405249778 1103 86-87 -0.10094213568209653 1103 88-89 -0.17823182808510651 1103 90-91 -0.13652266481043895 1103 92-93 -0.21343442207483676 1103 94-95 -0.20460842764970977 1103 96-97 -0.18149840980359144 1103 98-99 0.009309822548090096 1103 100-101 -0.007856795784132231 1104 1 0.196847762164289 1104 2 0.2223598615625484 1104 3 0.2427372844590714 1104 4 0.21842044174626096 1104 5 0.1949711309309805 1104 6 0.20231134857430533 1104 7 0.1399831171000585 1104 8 0.23403241519842055 1104 9 0.23506843588720017 1104 10-11 0.2576713799546795 1104 12-13 0.2795422777946115 1104 14-15 0.3632604579158851 1104 16-17 0.3715920514504276 1104 18-19 0.3560214191184272 1104 20-21 0.39851845397841856 1104 22-23 0.43876776612511037 1104 24-25 0.3405836036772385 1104 26-27 0.38280867773193705 1104 28-29 0.4192125378319602 1104 30-31 0.33360978938277697 1104 32-33 0.2987725662899905 1104 34-35 0.4143110847759104 1104 36-37 0.3567082290793735 1104 38-39 0.5019291398612964 1104 40-41 0.3044377042245898 1104 42-43 0.3623579554161651 1104 44-45 0.46041008960546037 1104 46-47 0.5129752936163854 1104 48-49 0.579085505024878 1104 50-51 0.5692754021898878 1104 52-53 0.5706705695861913 1104 54-55 0.6943156607246053 1104 56-57 0.6049037625923503 1104 58-59 0.47165214387403154 1104 60-61 0.4800584779722712 1104 62-63 0.471527842231275 1104 64-65 0.48054286997005846 1104 66-67 0.4878128924310303 1104 68-69 0.516341311885931 1104 70-71 0.48492799045287427 1104 72-73 0.39144693225383875 1104 74-75 0.27129024411030755 1104 76-77 0.2487771652853148 1104 78-79 0.3643985829944931 1104 80-81 0.2990049565527073 1104 82-83 0.3865929229296192 1104 84-85 0.35485342118690255 1104 86-87 0.28616170824827236 1104 88-89 0.21567746812730704 1104 90-91 0.21524257931927693 1104 92-93 0.2274612593956924 1104 94-95 0.28358323364003013 1104 96-97 0.3090409462857693 1104 98-99 0.3396733641277194 1104 100-101 0.6310052162673863 1105 1 0.18130372071351175 1105 2 0.17302442565355847 1105 3 0.15465438290466693 1105 4 0.22765675623150372 1105 5 0.21096572430786864 1105 6 0.22303673717534167 1105 7 0.2005823511617777 1105 8 0.154059448313987 1105 9 0.16523288734023112 1105 10-11 0.2184733623831434 1105 12-13 0.17861864634608793 1105 14-15 0.3178673513603556 1105 16-17 0.2590667079045872 1105 18-19 0.19292162186679462 1105 20-21 0.22640761820459687 1105 22-23 0.305629671959764 1105 24-25 0.18390417137266724 1105 26-27 0.24189856284119315 1105 28-29 0.2118460138400664 1105 30-31 0.1260179894278366 1105 32-33 0.24729700873198368 1105 34-35 0.2506480976166401 1105 36-37 0.24621036920102313 1105 38-39 0.2332875539185153 1105 40-41 0.24440166007050124 1105 42-43 0.16602995361395756 1105 44-45 0.20438395759374117 1105 46-47 0.2785756540579243 1105 48-49 0.127632475063173 1105 50-51 0.2815979973689764 1105 52-53 0.404980098759431 1105 54-55 0.3919953183051419 1105 56-57 0.371405226886111 1105 58-59 0.4101518059600906 1105 60-61 0.4740886649513172 1105 62-63 0.26607616392534084 1105 64-65 0.20897269650756556 1105 66-67 0.19495414045986337 1105 68-69 0.18146859280506078 1105 70-71 0.21955290597438903 1105 72-73 0.30268820281027686 1105 74-75 0.2169987370141122 1105 76-77 0.12273526395618717 1105 78-79 -9.79252280792764E-5 1105 80-81 0.0817262901864062 1105 82-83 0.19026492112741167 1105 84-85 0.048478111432455506 1105 86-87 0.17217207094257247 1105 88-89 0.0014400272622445698 1105 90-91 0.1819017367871716 1105 92-93 0.17925220330197789 1105 94-95 0.3669353399702828 1105 96-97 0.2816699167746215 1105 98-99 0.3410250199060485 1105 100-101 0.5723208278916267 1106 1 0.2026490934245011 1106 2 0.25650928297321585 1106 3 0.25570465181018776 1106 4 0.2631625084746929 1106 5 0.18414663374975504 1106 6 0.19488535892056547 1106 7 0.2166539031230812 1106 8 0.28810789369150314 1106 9 0.24906005110044305 1106 10-11 0.25330362066961953 1106 12-13 0.1849896149787824 1106 14-15 0.19186531642742466 1106 16-17 0.2031979351345754 1106 18-19 0.19679986187842502 1106 20-21 0.19565639607607466 1106 22-23 0.21266015716616948 1106 24-25 0.16669231718195476 1106 26-27 0.0218385988493921 1106 28-29 0.1173782469259379 1106 30-31 0.12311031121643623 1106 32-33 0.1972606743135472 1106 34-35 0.2948860034184122 1106 36-37 0.35759615544367307 1106 38-39 0.3085929672459642 1106 40-41 0.37780114627690153 1106 42-43 0.29197249719791785 1106 44-45 0.20238313714742162 1106 46-47 0.33033731465771865 1106 48-49 0.3640961253609021 1106 50-51 0.44278677631417906 1106 52-53 0.3857635967428692 1106 54-55 0.38624181644552635 1106 56-57 0.4469774779221325 1106 58-59 0.2976513018505358 1106 60-61 0.44947804944447256 1106 62-63 0.275728327049805 1106 64-65 0.2578688828192668 1106 66-67 0.24127514252433713 1106 68-69 0.43027757137507905 1106 70-71 0.29278426490218834 1106 72-73 0.1892118453556293 1106 74-75 0.19784611884248804 1106 76-77 -0.04509938708312333 1106 78-79 0.2380586084298706 1106 80-81 0.2043431492024439 1106 82-83 0.29451061526122047 1106 84-85 0.35723572091798417 1106 86-87 0.41640632917458476 1106 88-89 0.3869832955309853 1106 90-91 0.4877662840274404 1106 92-93 0.5249195197669287 1106 94-95 0.6377424521148143 1106 96-97 0.805673288978543 1106 98-99 0.8182626434064701 1106 100-101 1.0481399577510189 1107 1 0.18208616316272952 1107 2 0.17063132030271078 1107 3 0.19637400548510797 1107 4 0.19462652146664539 1107 5 0.15617964411364227 1107 6 0.1805767859008114 1107 7 0.07846982882994524 1107 8 0.21849745907503149 1107 9 0.15769623180886327 1107 10-11 0.15902964643806428 1107 12-13 0.26922694264541747 1107 14-15 0.2649275251400809 1107 16-17 0.1608655123758993 1107 18-19 0.35613340456262677 1107 20-21 0.2614985658454074 1107 22-23 0.23727824426832456 1107 24-25 0.11148977021520778 1107 26-27 0.2770609884168067 1107 28-29 0.08424366988582932 1107 30-31 0.12966045278234617 1107 32-33 0.29432800907371615 1107 34-35 0.2672492495357659 1107 36-37 0.3073202137204589 1107 38-39 0.33768323608906314 1107 40-41 0.3504462538690234 1107 42-43 0.38972464304433885 1107 44-45 0.47065329428252767 1107 46-47 0.47312626967167404 1107 48-49 0.4767351760055192 1107 50-51 0.5950766276750628 1107 52-53 0.5717641719496243 1107 54-55 0.4405784790674545 1107 56-57 0.4601611203020681 1107 58-59 0.5118774810752029 1107 60-61 0.2643027333348229 1107 62-63 0.36508334344217985 1107 64-65 0.4516194290624398 1107 66-67 0.37860534982235095 1107 68-69 0.4300704562910411 1107 70-71 0.5303349257802026 1107 72-73 0.3659478315506668 1107 74-75 0.27695379124699215 1107 76-77 0.2425807461290681 1107 78-79 0.2569166502472022 1107 80-81 0.2622856773072897 1107 82-83 0.48041416243732016 1107 84-85 0.3848743294867383 1107 86-87 0.4673794804336353 1107 88-89 0.5115853764379814 1107 90-91 0.4790992596350172 1107 92-93 0.5232959580378953 1107 94-95 0.5132660071364938 1107 96-97 0.39801521382768 1107 98-99 0.4113394304600462 1107 100-101 0.49936753129313516 1108 1 0.055781713757195206 1108 2 -0.06244328641552599 1108 3 0.01339631308422895 1108 4 -0.059639439109993475 1108 5 0.032624705809986665 1108 6 0.12437729816220155 1108 7 0.05193953445270694 1108 8 0.14289347520433893 1108 9 0.07809885887910895 1108 10-11 0.1683587311169532 1108 12-13 0.14659896382811866 1108 14-15 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-2.217284717300984 2316 62-63 -2.4258908245723347 2316 64-65 -2.5613728848037596 2316 66-67 -2.487381937707898 2316 68-69 -2.51899800424955 2316 70-71 -2.45548356868089 2316 72-73 -2.520054793788269 2316 74-75 -2.499355100302889 2316 76-77 -2.4969144432621775 2316 78-79 -2.4820128834159334 2316 80-81 -2.360557858378872 2316 82-83 -2.3212685186532767 2316 84-85 -2.322753358757673 2316 86-87 -2.5023227388643647 2316 88-89 -2.7137249871538707 2316 90-91 -2.960659728938264 2316 92-93 -3.166313843155674 2316 94-95 -3.2337728140669455 2316 96-97 -3.284454815885958 2316 98-99 -3.525446758404982 2316 100-101 -3.8521117883298572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17040.0 3 5998.0 4 6153.0 5 6943.0 6 7935.0 7 9216.0 8 11312.0 9 13831.0 10 16515.0 11 20430.0 12 24908.0 13 30082.0 14 31747.0 15 30336.0 16 35758.0 17 42573.0 18 38237.0 19 30877.0 20 31968.0 21 34014.0 22 37152.0 23 41538.0 24 47281.0 25 54211.0 26 61588.0 27 71921.0 28 84021.0 29 98390.0 30 117027.0 31 140134.0 32 171400.0 33 214963.0 34 283008.0 35 389720.0 36 569495.0 37 814713.0 38 713782.0 39 113283.0 40 73.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.961732993082556 18.251113408433824 13.387704134217232 23.399449464266386 2 15.61154617232724 19.970563943249147 39.72830770443547 24.68958217998814 3 19.78803611273091 24.2386193750767 29.05645682120902 26.916887690983373 4 12.767254085702723 15.71923575426902 36.668085885033456 34.8454242749948 5 13.870639593167214 37.42481709572269 33.750938351194584 14.953604959915518 6 32.749468917428665 35.26663579363355 17.289577486351664 14.694317802586127 7 29.39044256851886 32.377906536884886 20.228256679981786 18.003394214614463 8 26.844749426740528 34.40456673581894 19.58183620313755 19.16884763430299 9 27.028937559374423 16.485517789718074 20.128790449593634 36.35675420131387 10-11 25.482510710988482 26.136826449473936 26.899793765696028 21.480869073841554 12-13 26.41146355705101 23.56143396985811 27.444122065106598 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22.467816013542848 26.37337616252496 28.74089077061414 22.41791705331805 40-41 22.610550305540535 26.27025045919842 28.73964135109275 22.379557884168293 42-43 22.427698837119 26.45065097711473 28.24268828749203 22.878961898274238 44-45 22.49340090613618 26.484801739436165 28.289734278285504 22.732063076142143 46-47 22.64698381204578 26.736454521165598 27.89661802940581 22.719943637382812 48-49 22.86418650529328 26.662695962191517 27.742678114257135 22.73043941825807 50-51 22.522215983592968 26.879184729184473 27.808807932838626 22.78979135438393 52-53 22.86670144072522 26.88656762055507 27.587594654387825 22.659136284331886 54-55 22.406062890279397 26.63465054380161 27.822373465255584 23.136913100663406 56-57 22.600011260548758 26.99699707461732 27.584590939214053 22.818400725619874 58-59 22.865523029664303 26.591279355883984 27.616777487399247 22.92642012705247 60-61 22.63332663908998 26.637730912083917 28.13058087611921 22.598361572706896 62-63 22.50638325562389 26.636677531041354 28.05396875595998 22.802970457374776 64-65 22.357850634950545 26.7381256804969 27.872793121297885 23.031230563254674 66-67 22.407845733377645 26.841151467915616 27.569465088573242 23.181537710133497 68-69 22.298498346138725 26.66255337829389 27.87615336246144 23.162794913105937 70-71 22.404399111614055 26.676810482162566 27.726281597475534 23.19250880874785 72-73 22.487396987419586 26.528231220392563 27.79552571972483 23.188846072463022 74-75 22.26526269968272 27.039502928953457 27.528654907382656 23.16657946398117 76-77 22.503112128223616 26.786659465450178 27.48674359828902 23.22348480803719 78-79 22.49976654677678 26.824207981144845 27.5769555654518 23.099069906626575 80-81 22.485505669002535 27.0302823662336 27.457973435684906 23.026238529078956 82-83 22.56531335021546 26.939287837303063 27.425577223489572 23.0698215889919 84-85 22.471490607460847 26.84330168357873 27.332860995905182 23.352346713055237 86-87 22.463608227201785 26.743306560888254 27.531946246790202 23.261138965119763 88-89 22.61807442261187 26.733322271655034 27.247442998632128 23.40116030710097 90-91 22.580023945633297 26.780031815090243 27.41502953856877 23.22491470070769 92-93 22.74830553281801 26.686336190338395 27.402245693890904 23.163112582952696 94-95 22.58319944155012 26.511731078817185 27.434041315532166 23.471028164100527 96-97 22.59872777316006 26.382927484374303 27.498225585455614 23.520119157010026 98-99 22.572419108742547 26.484919440388495 27.189751398196837 23.75291005267212 100-101 22.816768325342533 25.97802473013137 27.58534950534904 23.619857439177057 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3287.0 1 1951.0 2 843.5 3 1110.0 4 1001.0 5 1012.0 6 1754.0 7 3300.5 8 5173.5 9 6030.5 10 5382.5 11 4860.0 12 5093.0 13 5436.5 14 5829.5 15 6350.5 16 6886.5 17 7369.5 18 7741.0 19 8089.0 20 8712.0 21 9738.0 22 11223.0 23 13240.5 24 15161.5 25 18014.0 26 22436.0 27 27468.0 28 32340.5 29 37637.0 30 45032.0 31 53709.5 32 63192.5 33 71834.0 34 80345.5 35 88298.5 36 96959.0 37 106312.5 38 112868.5 39 116866.5 40 119492.5 41 126389.0 42 133680.0 43 139922.0 44 148296.0 45 155669.5 46 159793.5 47 166735.0 48 186223.5 49 188608.5 50 173081.5 51 164754.5 52 158656.5 53 157881.5 54 156770.0 55 147771.0 56 134847.0 57 121044.0 58 109721.5 59 97726.5 60 83235.0 61 69009.5 62 56949.0 63 45536.5 64 34959.0 65 27891.5 66 21423.5 67 16992.5 68 12697.5 69 8356.0 70 6224.5 71 4838.5 72 3814.5 73 3132.0 74 2534.0 75 2073.5 76 1538.0 77 935.0 78 557.5 79 399.5 80 301.0 81 219.0 82 163.0 83 117.5 84 86.0 85 56.5 86 42.5 87 29.5 88 21.0 89 19.5 90 18.0 91 17.0 92 15.5 93 12.0 94 11.0 95 10.0 96 8.5 97 7.5 98 4.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.164982202997915 2 0.09622843166450128 3 0.08951638109501736 4 0.10457374787255964 5 0.12587332167972198 6 0.040540785439682946 7 0.04409817224150942 8 0.047812173556623865 9 0.048349137602182575 10-11 0.04657044420126934 12-13 0.05075428905624765 14-15 0.0487742341382499 16-17 0.04736470351865827 18-19 0.05159329537743314 20-21 0.05423336860143015 22-23 0.06804900602361792 24-25 0.0846501444321415 26-27 0.08649595833874958 28-29 0.07604753295225293 30-31 0.06348481163636885 32-33 0.05283502473278767 34-35 0.3050179513792481 36-37 0.48282017096487745 38-39 0.4605026028213433 40-41 0.434650021377881 42-43 0.4662078458054047 44-45 0.47510131280997087 46-47 0.4750901260590217 48-49 0.49220585501120573 50-51 0.48059400752599857 52-53 0.4880331969071766 54-55 0.47873700686844134 56-57 0.45667673399673747 58-59 0.4350079974082536 60-61 0.4024321786443582 62-63 0.46153178390866423 64-65 0.5023969851258722 66-67 0.5038288892473621 68-69 0.5147919051775192 70-71 0.5113911328889806 72-73 0.501658659563229 74-75 0.4940069219140173 76-77 0.47544810208939425 78-79 0.44911449035511897 80-81 0.4549316008486717 82-83 0.46725940039462377 84-85 0.46483187543866045 86-87 0.4761193071463426 88-89 0.4874067388540248 90-91 0.49217229475835833 92-93 0.4940852291706613 94-95 0.49809008601045335 96-97 0.49963385764143464 98-99 0.4935370783741534 100-101 0.47960957344247424 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4469573.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.472566902550575 #Duplication Level Percentage of deduplicated Percentage of total 1 70.04014276025165 31.14864934769485 2 14.485555493988986 12.884196716540686 3 5.950148548504859 7.938551382104897 4 2.991368660536807 5.321353715436651 5 1.7214164276995167 3.8277903624008194 6 1.08941573886488 2.9069468598815966 7 0.7342095396766308 2.285652801163188 8 0.5325411504610664 1.8946777553792826 9 0.39000638065529913 1.561012637050296 >10 1.831981878374309 14.558789537977324 >50 0.13900847733501617 4.32365575290097 >100 0.08651874158530783 7.136897963146365 >500 0.004905010791009251 1.4685437829611938 >1k 0.002578922818271054 1.7819657476653397 >5k 1.0113422816748165E-4 0.34724483031964776 >10k+ 1.0113422816748165E-4 0.6140708073770029 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15463 0.34596145985310006 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11843 0.26496938298132733 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7846 0.17554249589390306 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7595 0.16992674691743484 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.237350189827977E-5 0.0 0.0 0.0 0.0 3 2.237350189827977E-5 0.0 0.0 0.0 0.0 4 2.237350189827977E-5 0.0 0.0 0.0 0.0 5 2.237350189827977E-5 0.0 0.0 0.0 2.237350189827977E-5 6 2.237350189827977E-5 0.0 0.0 0.0 2.237350189827977E-5 7 4.474700379655954E-5 0.0 0.0 0.0 2.237350189827977E-5 8 4.474700379655954E-5 0.0 0.0 0.0 2.237350189827977E-5 9 4.474700379655954E-5 0.0 0.0 0.0 2.237350189827977E-5 10-11 4.474700379655954E-5 0.0 0.0 0.0 2.237350189827977E-5 12-13 4.474700379655954E-5 0.0 0.0 1.1186750949139885E-5 2.237350189827977E-5 14-15 4.474700379655954E-5 0.0 0.0 3.3560252847419655E-5 2.237350189827977E-5 16-17 4.474700379655954E-5 0.0 0.0 5.5933754745699425E-5 2.237350189827977E-5 18-19 4.474700379655954E-5 0.0 0.0 6.712050569483931E-5 2.237350189827977E-5 20-21 4.474700379655954E-5 0.0 0.0 1.2305426044053873E-4 2.237350189827977E-5 22-23 4.474700379655954E-5 0.0 0.0 3.1322902657591675E-4 2.237350189827977E-5 24-25 4.474700379655954E-5 0.0 0.0 6.264580531518335E-4 2.237350189827977E-5 26-27 4.474700379655954E-5 0.0 0.0 0.001062741340168289 4.474700379655954E-5 28-29 6.712050569483931E-5 0.0 0.0 0.003915362832198959 4.474700379655954E-5 30-31 8.949400759311908E-5 0.0 0.0 0.01364783615795066 4.474700379655954E-5 32-33 8.949400759311908E-5 0.0 0.0 0.032329710243014266 4.474700379655954E-5 34-35 8.949400759311908E-5 0.0 0.0 0.061325768703184846 4.474700379655954E-5 36-37 8.949400759311908E-5 0.0 0.0 0.11186750949139884 4.474700379655954E-5 38-39 1.1186750949139884E-4 0.0 0.0 0.18499530044592627 4.474700379655954E-5 40-41 1.1186750949139884E-4 0.0 0.0 0.26408562965634524 4.474700379655954E-5 42-43 1.342410113896786E-4 0.0 0.0 0.34405971219174625 4.474700379655954E-5 44-45 1.5661451328795838E-4 0.0 0.0 0.4426038013027195 4.474700379655954E-5 46-47 1.7898801518623816E-4 0.0 0.0 0.5674255683932223 4.474700379655954E-5 48-49 1.7898801518623816E-4 0.0 0.0 0.7008611337145629 4.474700379655954E-5 50-51 1.7898801518623816E-4 0.0 0.0 0.8409640026015908 4.474700379655954E-5 52-53 1.7898801518623816E-4 0.0 0.0 0.9821631730816344 4.474700379655954E-5 54-55 1.7898801518623816E-4 0.0 0.0 1.1394936384303378 4.474700379655954E-5 56-57 1.7898801518623816E-4 0.0 0.0 1.3303843566264608 4.474700379655954E-5 58-59 1.7898801518623816E-4 0.0 0.0 1.5406840877193413 4.474700379655954E-5 60-61 1.7898801518623816E-4 0.0 0.0 1.7730776519367732 4.474700379655954E-5 62-63 1.7898801518623816E-4 0.0 0.0 2.0021711246242093 4.474700379655954E-5 64-65 1.7898801518623816E-4 0.0 0.0 2.2412767394111253 4.474700379655954E-5 66-67 1.7898801518623816E-4 0.0 0.0 2.5187864702064378 4.474700379655954E-5 68-69 1.7898801518623816E-4 0.0 0.0 2.8346779435082503 4.474700379655954E-5 70-71 1.7898801518623816E-4 0.0 0.0 3.1779545831335567 4.474700379655954E-5 72-73 1.7898801518623816E-4 0.0 0.0 3.5212424095098123 4.474700379655954E-5 74-75 1.7898801518623816E-4 0.0 0.0 3.868859508503385 4.474700379655954E-5 76-77 1.7898801518623816E-4 0.0 0.0 4.248772757487124 4.474700379655954E-5 78-79 1.7898801518623816E-4 0.0 0.0 4.676061896740471 4.474700379655954E-5 80-81 1.7898801518623816E-4 2.237350189827977E-5 0.0 5.142560598070554 4.474700379655954E-5 82-83 1.7898801518623816E-4 2.237350189827977E-5 0.0 5.6208613216519785 4.474700379655954E-5 84-85 1.7898801518623816E-4 3.3560252847419655E-5 0.0 6.095761272944865 4.474700379655954E-5 86-87 1.7898801518623816E-4 4.474700379655954E-5 0.0 6.592229280067693 4.474700379655954E-5 88-89 1.7898801518623816E-4 4.474700379655954E-5 2.237350189827977E-5 7.128958403856476 4.474700379655954E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 20705 0.0 28.753962 1 TACATGG 20850 0.0 27.992376 2 ACATGGG 20945 0.0 26.50833 3 GTATCAA 26445 0.0 24.125969 1 GGTATCA 19650 0.0 22.940413 1 CATGGGA 11555 0.0 22.09805 4 CATGGGG 9765 0.0 22.025143 4 TACCTGG 3135 0.0 21.76006 2 GAGTACT 13930 0.0 21.428747 12-13 ATCAACG 30545 0.0 20.643011 3 TCAACGC 30605 0.0 20.618021 4 CAACGCA 30820 0.0 20.412706 5 TATCAAC 31225 0.0 20.238976 2 AACGCAG 31415 0.0 20.147816 6 GTACTTT 15260 0.0 19.6083 14-15 AGTACTT 14510 0.0 19.28337 12-13 ATGGGAG 5440 0.0 19.158386 5 GTACCTG 3880 0.0 18.32495 1 ACGCAGA 34710 0.0 18.085978 7 CGCAGAG 34900 0.0 17.933054 8 >>END_MODULE