FastQCFastQC Report
Thu 26 May 2016
SRR938705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938705_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4469573
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152840.341956603013308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129340.28937887355235054No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT87780.1963945996630998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83490.18679636734873778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG200900.025.3959851
TACATGG203950.024.6620452
ACATGGG200400.024.1044373
GTATCAA277850.023.437631
GGTATCA209700.022.6491681
GAGTACT158150.020.76386312-13
CATGGGG92900.020.0224514
AGTACTT163100.019.7845512-13
ATCAACG327500.019.6614483
TCAACGC328050.019.6429464
TATCAAC333900.019.4977552
CAACGCA331650.019.4440335
GTACTTT169750.019.35892514-15
CATGGGA109950.019.29124
AACGCAG337600.019.1294566
TGTACCG6050.018.0392885
AGTACAT155250.018.0329722
AGAGTAC285450.017.92921610-11
GTACCGT4600.017.5363036
GAGTACA186450.017.5273911
Produced by FastQC (version 0.11.5)