##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938705_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4469573 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02126086764888 33.0 31.0 34.0 30.0 34.0 2 32.24687123356079 34.0 31.0 34.0 30.0 34.0 3 32.33982686936761 34.0 31.0 34.0 30.0 34.0 4 35.95939231778964 37.0 35.0 37.0 35.0 37.0 5 35.86875301063435 37.0 35.0 37.0 35.0 37.0 6 35.899040020154054 37.0 35.0 37.0 35.0 37.0 7 35.83118141263159 37.0 35.0 37.0 35.0 37.0 8 35.79791514759911 37.0 35.0 37.0 33.0 37.0 9 37.457690924837785 39.0 37.0 39.0 34.0 39.0 10-11 37.38478317727443 39.0 37.0 39.0 34.0 39.0 12-13 37.310900168763325 39.0 37.0 39.0 34.0 39.0 14-15 38.55041835092524 40.0 38.0 41.0 34.0 41.0 16-17 38.486059182834694 40.0 38.0 41.0 34.0 41.0 18-19 38.50220669849223 40.0 38.0 41.0 33.5 41.0 20-21 38.5726006712498 40.0 38.0 41.0 34.0 41.0 22-23 38.52971861070398 40.0 38.0 41.0 34.0 41.0 24-25 38.45835407543405 40.0 38.0 41.0 34.0 41.0 26-27 38.30445357979386 40.0 38.0 41.0 33.5 41.0 28-29 38.14664834873488 40.0 38.0 41.0 33.0 41.0 30-31 37.93400096608781 40.0 37.0 41.0 33.0 41.0 32-33 37.722739733750856 40.0 37.0 41.0 33.0 41.0 34-35 37.68114347835912 40.0 37.0 41.0 33.0 41.0 36-37 37.597521955676754 40.0 37.0 41.0 32.0 41.0 38-39 37.500236599782575 40.0 37.0 41.0 32.0 41.0 40-41 37.345228056460876 40.0 36.0 41.0 31.5 41.0 42-43 37.16851251786244 40.0 36.0 41.0 31.0 41.0 44-45 36.924278784572934 40.0 36.0 41.0 31.0 41.0 46-47 36.640390592121435 39.5 36.0 41.0 30.0 41.0 48-49 36.36368015468145 39.0 35.0 41.0 30.0 41.0 50-51 36.125894017168974 39.0 35.0 41.0 29.5 41.0 52-53 35.91401449310706 39.0 35.0 41.0 28.5 41.0 54-55 35.67296540407775 39.0 35.0 40.0 28.0 41.0 56-57 35.41132631685398 38.0 35.0 40.0 28.0 41.0 58-59 35.10732009523058 38.0 34.0 40.0 27.0 41.0 60-61 34.76448298305006 38.0 34.0 40.0 26.0 41.0 62-63 34.401688819043784 37.0 34.0 40.0 26.0 41.0 64-65 34.03875291890299 36.5 33.0 39.5 26.0 41.0 66-67 33.64484784116962 36.0 33.0 39.0 25.5 40.5 68-69 33.21252947429207 35.5 32.5 39.0 24.5 40.0 70-71 32.73955151867975 35.0 32.0 38.0 23.5 40.0 72-73 32.39296237023089 35.0 32.0 37.5 22.5 39.5 74-75 31.97604256603483 35.0 31.5 37.0 22.0 39.0 76-77 29.94049219019356 33.0 29.0 35.0 19.0 37.5 78-79 30.99578158808459 34.0 30.5 36.0 20.0 38.0 80-81 31.04994257840738 34.0 31.0 36.0 20.0 37.0 82-83 30.75314409228801 34.0 31.0 35.0 18.5 37.0 84-85 30.378911363568733 34.0 31.0 35.0 17.5 36.5 86-87 29.85923107196146 34.0 30.0 35.0 9.0 36.0 88-89 29.353886601695507 34.0 29.5 35.0 4.5 36.0 90-91 28.94854609153939 34.0 29.0 35.0 2.0 35.0 92-93 28.673602153941776 34.0 29.0 35.0 2.0 35.0 94-95 28.29705678819878 34.0 29.0 35.0 2.0 35.0 96-97 27.790870962393946 33.5 28.5 35.0 2.0 35.0 98-99 27.06943437326116 33.0 27.0 35.0 2.0 35.0 100-101 23.91695067962868 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04799163507225046 1101 2 0.05531058367131436 1101 3 0.10818437073622533 1101 4 0.12302523678987143 1101 5 0.09981251469484675 1101 6 0.10463983093251272 1101 7 0.08549479470723043 1101 8 0.07034055976242826 1101 9 0.20015218752283914 1101 10-11 0.1640062181102877 1101 12-13 0.19885900547415503 1101 14-15 0.24590932532241538 1101 16-17 0.20808434902560435 1101 18-19 0.20199171167784158 1101 20-21 0.2013415376839518 1101 22-23 0.22598159717141186 1101 24-25 0.18275345104835594 1101 26-27 0.22454968117604324 1101 28-29 0.21102868621186133 1101 30-31 0.19968178361185096 1101 32-33 0.15954661094939837 1101 34-35 0.17393901921787602 1101 36-37 0.2013298065024074 1101 38-39 0.1674748368367034 1101 40-41 0.2027891706722471 1101 42-43 0.18481066282167546 1101 44-45 0.19854012078458538 1101 46-47 0.20693232774225834 1101 48-49 0.19920649384120281 1101 50-51 0.2665028700107044 1101 52-53 0.3269492580230917 1101 54-55 0.23203207130550396 1101 56-57 0.14563606848730615 1101 58-59 0.29406413134848464 1101 60-61 0.30507168595644885 1101 62-63 0.19836775688251862 1101 64-65 0.13203649020799446 1101 66-67 0.15009791612355627 1101 68-69 0.17279649688966714 1101 70-71 0.024080603856887706 1101 72-73 0.0539366767136471 1101 74-75 0.0361677754107923 1101 76-77 -0.05583267649750567 1101 78-79 0.13244046301342038 1101 80-81 0.1008510327016161 1101 82-83 0.04632075311251782 1101 84-85 0.08567260666176324 1101 86-87 0.11299935595288346 1101 88-89 0.29653824049951183 1101 90-91 0.1611191456646388 1101 92-93 0.09371770034811178 1101 94-95 0.19109216962804965 1101 96-97 0.0324452870167562 1101 98-99 0.15919257152549804 1101 100-101 0.632484507465005 1102 1 0.0959758424407724 1102 2 0.1412974727477092 1102 3 0.1398487868157119 1102 4 0.11763680866011583 1102 5 0.10852806527606873 1102 6 0.1286500870667524 1102 7 0.1618049504225283 1102 8 0.1286945652535465 1102 9 0.12158632605452624 1102 10-11 0.1678174146891891 1102 12-13 0.18531208007176048 1102 14-15 0.30287994526553064 1102 16-17 0.3009154241202836 1102 18-19 0.2135425535768647 1102 20-21 0.27915312264084235 1102 22-23 0.2569411810581599 1102 24-25 0.14527771807723155 1102 26-27 0.18066515714225062 1102 28-29 0.2960136814178185 1102 30-31 0.2947952654470001 1102 32-33 0.3284336810753743 1102 34-35 0.30246852301748817 1102 36-37 0.2673283416419139 1102 38-39 0.22489330516041406 1102 40-41 0.28128025882191565 1102 42-43 0.2713972951253325 1102 44-45 0.2545157331791188 1102 46-47 0.27714545016944925 1102 48-49 0.2769076074201351 1102 50-51 0.23464732551899914 1102 52-53 0.24265993963176413 1102 54-55 0.31450200235257597 1102 56-57 0.19291438828809504 1102 58-59 0.27179516480862276 1102 60-61 0.1650158777265318 1102 62-63 0.09151172427988286 1102 64-65 0.22999154284218548 1102 66-67 0.13966220983230215 1102 68-69 0.26936908742897714 1102 70-71 0.23790137268701983 1102 72-73 0.2016469087325703 1102 74-75 0.26474511408220636 1102 76-77 0.10350620494219598 1102 78-79 0.11069852436342487 1102 80-81 0.17567635494718203 1102 82-83 0.37085252280225234 1102 84-85 0.41419572544263517 1102 86-87 0.3355796053485527 1102 88-89 0.5376413764935499 1102 90-91 0.6195280306556761 1102 92-93 0.6679063940102168 1102 94-95 0.6398741085735509 1102 96-97 0.6025182556441493 1102 98-99 0.8072678841407637 1102 100-101 1.2806091324770712 1103 1 0.08072984571576569 1103 2 0.04490858503402251 1103 3 0.0036935302339102805 1103 4 0.09013635282494903 1103 5 0.05038944948985602 1103 6 0.09207559627358819 1103 7 0.1134277081494659 1103 8 0.07079405691607121 1103 9 0.04014413684047469 1103 10-11 0.07195940597738826 1103 12-13 0.06652868376076526 1103 14-15 0.15641878949041654 1103 16-17 0.04022891416872909 1103 18-19 0.14251489524263405 1103 20-21 0.1986702252565422 1103 22-23 0.05235312139085835 1103 24-25 0.11163656658857946 1103 26-27 0.184412782646028 1103 28-29 0.1412407182890263 1103 30-31 0.11077233717012547 1103 32-33 0.17022982952357069 1103 34-35 0.06513109261941707 1103 36-37 0.12029673407766239 1103 38-39 0.21711671072535665 1103 40-41 0.12044897025457146 1103 42-43 0.2360927709187095 1103 44-45 0.1605602881132171 1103 46-47 0.2179994309183968 1103 48-49 0.2027220200253126 1103 50-51 0.25557111431567847 1103 52-53 0.23251604637324874 1103 54-55 0.17482458712527915 1103 56-57 0.12475451876787957 1103 58-59 -0.07203640247083598 1103 60-61 0.06464396575828601 1103 62-63 0.00520806716616562 1103 64-65 0.034496195250191874 1103 66-67 0.023879096360978735 1103 68-69 0.06984895003968461 1103 70-71 0.03168185134335744 1103 72-73 0.15835845861032283 1103 74-75 0.09832513670732013 1103 76-77 0.11348757632346818 1103 78-79 0.049578999377210664 1103 80-81 0.16676636389671984 1103 82-83 0.15869424568687407 1103 84-85 0.18008236792949006 1103 86-87 0.20516877480809725 1103 88-89 0.3085027362516648 1103 90-91 0.2747430790877097 1103 92-93 0.34316033831298753 1103 94-95 0.22289624260859142 1103 96-97 0.3594076870064171 1103 98-99 0.5071537731452338 1103 100-101 1.008586721587303 1104 1 -0.02741694252590321 1104 2 0.04537269547255107 1104 3 -0.004145131859154105 1104 4 -0.026568363802908834 1104 5 -0.0026276086025376344 1104 6 0.0015072914810900784 1104 7 0.013982261540512297 1104 8 -0.014497530661408575 1104 9 -0.010612026513918238 1104 10-11 0.04868606590641633 1104 12-13 -0.01881539828368517 1104 14-15 0.019227467572804358 1104 16-17 0.0954186855041641 1104 18-19 0.04735943871140336 1104 20-21 0.028184594644748984 1104 22-23 -0.008354017964094851 1104 24-25 0.01889483225426858 1104 26-27 -0.003716591349267162 1104 28-29 0.09231505644047644 1104 30-31 -0.06336181238906846 1104 32-33 0.004453581919143801 1104 34-35 0.1205856386786337 1104 36-37 -0.013943615983684765 1104 38-39 0.15744730921293382 1104 40-41 0.22154021561519244 1104 42-43 0.19705727642159587 1104 44-45 0.1818922794437512 1104 46-47 0.11336145778195572 1104 48-49 0.0895572519170571 1104 50-51 0.08274720311666783 1104 52-53 0.031677332143857484 1104 54-55 0.1304543807949088 1104 56-57 0.03037946974618677 1104 58-59 -0.05754578068923166 1104 60-61 0.04163481202282071 1104 62-63 -0.09114542463940722 1104 64-65 0.1579386995084633 1104 66-67 -0.003239975057951483 1104 68-69 0.14230778933789878 1104 70-71 0.07718309343101026 1104 72-73 -0.018935361568082953 1104 74-75 -0.006745071572410666 1104 76-77 0.009357791581994235 1104 78-79 0.06124077824278373 1104 80-81 0.05210448019570535 1104 82-83 0.0018691744931693677 1104 84-85 0.10981609358306699 1104 86-87 0.2357015215063818 1104 88-89 0.3518551587766616 1104 90-91 0.5373560899283305 1104 92-93 0.4227600367921376 1104 94-95 0.4914665568237915 1104 96-97 0.5209940728682767 1104 98-99 0.3877161976364718 1104 100-101 0.881095057130505 1105 1 -0.028318046378117856 1105 2 -0.014100158773899807 1105 3 -5.407164502813089E-4 1105 4 -0.05653006688919504 1105 5 -0.04700697332432213 1105 6 -0.06900408495503996 1105 7 -0.010122266506350286 1105 8 0.016365276277092278 1105 9 -0.01151313036613999 1105 10-11 0.042265700959355 1105 12-13 -0.04123035660763463 1105 14-15 -7.094551575335117E-4 1105 16-17 -0.06846957761815986 1105 18-19 -0.12587777687769375 1105 20-21 -0.03714543464112552 1105 22-23 -0.05757681589155794 1105 24-25 -0.16740838919199774 1105 26-27 -0.17684116895683388 1105 28-29 -0.08531504042818483 1105 30-31 -0.18613721225390378 1105 32-33 -0.09140134036064751 1105 34-35 0.016508143747337556 1105 36-37 -0.04424323301454791 1105 38-39 -0.03054548195625273 1105 40-41 -0.0420326611588564 1105 42-43 -0.10222184049663241 1105 44-45 -0.07559814632782036 1105 46-47 -0.03149098646224502 1105 48-49 -0.0014542371570556156 1105 50-51 0.1320826390256613 1105 52-53 0.12764489240517918 1105 54-55 0.1788887128516805 1105 56-57 0.08410778693474441 1105 58-59 0.14846908752432597 1105 60-61 0.09941809654635847 1105 62-63 -0.05487599458759007 1105 64-65 0.0805564061991646 1105 66-67 0.07695826778953574 1105 68-69 -0.005923794352121092 1105 70-71 -0.05877095027253176 1105 72-73 -0.15319983554871897 1105 74-75 -0.1237759343793492 1105 76-77 -0.12040116313754012 1105 78-79 -0.2522307243478892 1105 80-81 -0.1232728570424122 1105 82-83 -0.11516168831376916 1105 84-85 -0.08369595868095203 1105 86-87 0.010538196433170555 1105 88-89 0.1200461927933354 1105 90-91 0.2680386760963849 1105 92-93 0.11942595253892563 1105 94-95 0.050601497125661865 1105 96-97 0.12608530963331077 1105 98-99 0.3621473758297604 1105 100-101 0.6613383551706029 1106 1 -0.06498696237947144 1106 2 -0.10344491396674016 1106 3 -0.05505267033267103 1106 4 -0.030268181141863693 1106 5 0.006300820170828558 1106 6 -0.07394119905436014 1106 7 -0.12483473911425591 1106 8 -0.12582973359061356 1106 9 -0.12447312551640977 1106 10-11 -0.1102743047894208 1106 12-13 -0.04352349637660069 1106 14-15 0.002712093708467478 1106 16-17 -0.13040818901917817 1106 18-19 -0.19216069523147183 1106 20-21 -0.17438727596516657 1106 22-23 -0.1449696845844457 1106 24-25 -0.07728241189375495 1106 26-27 -0.14966859806490618 1106 28-29 -0.05600203964190342 1106 30-31 -0.06659579397089743 1106 32-33 -0.11872333317699457 1106 34-35 -0.09765827225593426 1106 36-37 0.006886576030034064 1106 38-39 0.0412184750712683 1106 40-41 0.08250780284797798 1106 42-43 0.07369579943325988 1106 44-45 0.19924983092779058 1106 46-47 0.1322511940600961 1106 48-49 0.23138090322171934 1106 50-51 0.18683284086213092 1106 52-53 0.2225414799495553 1106 54-55 0.23616544754723634 1106 56-57 0.15365199226928183 1106 58-59 0.11082290538679018 1106 60-61 0.08518417481549534 1106 62-63 0.1934307752761839 1106 64-65 0.09613116943463496 1106 66-67 0.10396821358202857 1106 68-69 0.07741510880310898 1106 70-71 0.2010113822866728 1106 72-73 0.23304571833347154 1106 74-75 0.3295573664169815 1106 76-77 0.13654711047026424 1106 78-79 0.14726809443031286 1106 80-81 0.10621723068677724 1106 82-83 0.3258717247095433 1106 84-85 0.19244405456845826 1106 86-87 0.2650849820380081 1106 88-89 0.4330248110990489 1106 90-91 0.4466656383669019 1106 92-93 0.5733948808957763 1106 94-95 0.5138103104761775 1106 96-97 0.5834918413005106 1106 98-99 0.6684631685481293 1106 100-101 0.8644516587051747 1107 1 -0.047299252901567 1107 2 -0.049527715284632734 1107 3 -0.13851064873497876 1107 4 -0.023242782613010604 1107 5 -0.015280307356370315 1107 6 -0.04497369518746552 1107 7 -0.017212385725919432 1107 8 0.030100383869452685 1107 9 -0.07577028789748397 1107 10-11 -0.06522667238515822 1107 12-13 -0.02419845476048721 1107 14-15 -0.01946593786489359 1107 16-17 0.022833319567261867 1107 18-19 0.007060590670683098 1107 20-21 0.04040691993440504 1107 22-23 -0.09709302070234571 1107 24-25 -0.0711864397638422 1107 26-27 -0.036008555422370137 1107 28-29 -0.020055987125317642 1107 30-31 -0.03302424780293478 1107 32-33 0.04609098479649276 1107 34-35 -0.07881862485971425 1107 36-37 -0.021819264655981385 1107 38-39 -0.03940475535493704 1107 40-41 0.0043074276424377445 1107 42-43 -0.047974480060311464 1107 44-45 -0.16482047844402103 1107 46-47 -0.16840119812929544 1107 48-49 -0.36791551017104496 1107 50-51 -0.31950596811434195 1107 52-53 -0.26593327192901484 1107 54-55 -0.43415456964799404 1107 56-57 -0.3413502892486875 1107 58-59 -0.24203585188205778 1107 60-61 -0.24042695695969485 1107 62-63 -0.20050480766435896 1107 64-65 -0.21160322731981296 1107 66-67 -0.2386136771612115 1107 68-69 -0.10159708537693746 1107 70-71 -0.21166892877213428 1107 72-73 -0.17246582441984515 1107 74-75 -0.19163986649298081 1107 76-77 -0.2235824482465567 1107 78-79 -0.3151920933350638 1107 80-81 -0.27304008693914383 1107 82-83 -0.21558439697412268 1107 84-85 -0.20710457072803834 1107 86-87 -0.2970193854682961 1107 88-89 -0.010415494431267547 1107 90-91 0.20496025195194179 1107 92-93 0.1530463320225124 1107 94-95 0.21819300996418178 1107 96-97 0.19314621927436093 1107 98-99 0.0983532136093821 1107 100-101 0.6576229331235126 1108 1 -0.21396591956823485 1108 2 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-2.5426054389176436 2316 100-101 -3.2730712507851756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 23.0 4 41.0 5 118.0 6 278.0 7 722.0 8 1591.0 9 2921.0 10 4773.0 11 7277.0 12 10339.0 13 14000.0 14 17069.0 15 21990.0 16 27688.0 17 36563.0 18 35161.0 19 25375.0 20 23746.0 21 25073.0 22 26823.0 23 29622.0 24 34638.0 25 41475.0 26 50775.0 27 62128.0 28 76155.0 29 92971.0 30 113142.0 31 137398.0 32 169256.0 33 212820.0 34 282786.0 35 397658.0 36 600609.0 37 946395.0 38 845719.0 39 94432.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.384990608516894 19.273093367200474 14.048477214029226 23.293438810253406 2 16.18727337040921 20.68293772134385 38.80565324696565 24.32413566128129 3 19.895748430554775 24.28097717611951 29.39323286586884 26.430041527456872 4 13.207727002109598 16.382571668479294 36.155422453106816 34.25427887630429 5 13.787290195282637 37.614085282867066 33.82871249669711 14.769912025153186 6 32.7623690227232 35.75594357671304 16.876421975880024 14.605265424683743 7 29.220956901252087 32.46838120777981 20.18391913500462 18.12674275596349 8 26.845204228681354 34.862234043386245 19.27598005447053 19.01658167346187 9 26.707025480957576 16.492336068792255 20.002760890134248 36.79787756011592 10-11 25.505971152054123 26.358737176907056 26.747991362933327 21.387300308105495 12-13 26.30287054266705 23.650234597354157 27.468406937306987 22.57848792267181 14-15 22.904338602343877 25.15980594516934 25.962390270275954 25.973465182210827 16-17 22.50362961768027 28.482402535060576 26.500359437867367 22.51360840939179 18-19 22.314094148930515 26.925446365895585 28.80226106888418 21.95819841628972 20-21 23.103221416191687 25.98736814032397 28.779850596416235 22.129559847068116 22-23 22.685959039040192 26.055441985173978 28.730697540906032 22.527901434879798 24-25 22.66521880278049 26.219339073329824 28.868551872852283 22.246890251037403 26-27 22.464730096428774 26.345820631230954 28.909613981752376 22.279835290587894 28-29 22.075844829025055 26.733582827710833 28.95043888979999 22.24013345346412 30-31 22.330880377163545 26.30988463551216 28.86394293146124 22.495292055863054 32-33 22.14471046786796 26.482988419699154 28.831490166957785 22.540810945475105 34-35 22.842747495495555 26.368492329998844 28.553672107800114 22.235088066705487 36-37 22.778324724103527 26.20938448006887 28.617205189683336 22.395085606144267 38-39 22.429095143481213 26.416667850740467 28.806714291420217 22.3475227143581 40-41 22.546999696368346 26.330637194631468 28.793991628537434 22.328371480462746 42-43 22.407010408417307 26.523380091540016 28.303592094842912 22.76601740519977 44-45 22.500035798315867 26.526429001647617 28.29579432435601 22.677740875680506 46-47 22.631499873419862 26.811508355352014 27.909405089002675 22.64758668222545 48-49 22.839294867449247 26.77067888618393 27.742450002472275 22.647576243894548 50-51 22.504918835302135 26.96916739343568 27.783174786567074 22.74273898469512 52-53 22.883013657009293 26.955953063077835 27.52144332355686 22.63958995635601 54-55 22.416313594162126 26.69361256656956 27.754743014601168 23.135330824667143 56-57 22.604821534406085 27.08145722197624 27.499886454477867 22.81383478913981 58-59 22.880187007347708 26.65548285881576 27.543781184578247 22.920548949258283 60-61 22.633086399423476 26.778638428772293 27.997239990294325 22.591035181509902 62-63 22.50263056448569 26.747532706144412 27.8746985450288 22.875138184341097 64-65 22.32012990950142 26.827696963445945 27.750078139455383 23.102094987597248 66-67 22.320627011331528 26.990792412743158 27.453817473261395 23.234763102663923 68-69 22.25709158628087 26.743817791519685 27.753407258175034 23.245683364024412 70-71 22.389227655464325 26.77865130679573 27.555296117089185 23.27682492065076 72-73 22.481621846203208 26.67640734360978 27.57632552371334 23.265645286473674 74-75 22.21764808405635 27.12412349009626 27.271911656885344 23.386316768962047 76-77 22.36949769503048 26.871038727317742 27.383723254498044 23.37574032315374 78-79 22.31817772334041 26.935564643786186 27.425534545938806 23.320723086934603 80-81 22.26517438358751 27.123939383905217 27.330424479122385 23.280461753384888 82-83 22.310744225455093 27.027604202907078 27.279708822296893 23.381942749340933 84-85 22.203698205622775 26.952093634000384 27.16416087174323 23.680047288633613 86-87 22.175071787237517 26.854650689223586 27.300774099969153 23.669503423569743 88-89 22.191221254902743 26.89452209278193 27.03173102469727 23.882525627618058 90-91 22.21275086187447 26.943459755799708 27.15048464556987 23.693304736755955 92-93 22.24720348006398 26.830885187466453 27.161934708304354 23.759976624165215 94-95 22.06915137166952 26.691563162151006 27.17745985269505 24.061825613484427 96-97 21.980927706173244 26.542974747584918 27.316147125927593 24.159950420314246 98-99 21.912671805049463 26.722346215162574 26.971050535236714 24.393931444551253 100-101 21.910248235280957 26.274613775436006 27.491134454251547 24.32400353503149 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1495.0 1 1023.5 2 755.5 3 997.0 4 863.5 5 832.5 6 1500.0 7 3033.0 8 4678.5 9 5350.0 10 4714.5 11 4018.0 12 4114.0 13 4403.5 14 4795.5 15 5165.0 16 5516.5 17 5898.0 18 6302.5 19 6881.5 20 7655.0 21 8797.5 22 10530.0 23 12661.0 24 14874.0 25 17848.0 26 22424.0 27 27741.0 28 32429.5 29 38167.0 30 45968.5 31 54347.5 32 64013.0 33 72586.5 34 80556.5 35 88298.5 36 97494.5 37 107064.5 38 113461.0 39 117165.5 40 120000.0 41 126567.0 42 133729.5 43 140633.0 44 149122.0 45 156752.5 46 159761.0 47 167312.5 48 188616.0 49 190696.5 50 175013.0 51 166689.5 52 160492.0 53 159974.5 54 159226.5 55 149868.0 56 135745.5 57 122057.0 58 110521.0 59 97724.0 60 83076.0 61 68617.0 62 56370.5 63 44776.5 64 34505.5 65 27201.5 66 20217.5 67 16008.5 68 11750.5 69 7360.5 70 5299.5 71 4139.5 72 3385.0 73 2963.5 74 2463.0 75 1953.0 76 1375.0 77 757.5 78 420.0 79 269.5 80 193.5 81 120.0 82 70.0 83 40.0 84 23.5 85 14.5 86 8.0 87 4.5 88 7.5 89 6.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39527265803690864 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.5729527183021735E-4 16-17 0.002103109178438298 18-19 1.2305426044053873E-4 20-21 3.3560252847419655E-5 22-23 0.0 24-25 0.0 26-27 1.0068075854225896E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008401249962804053 36-37 0.01840220531133511 38-39 0.012048130772223655 40-41 0.0076517376492116805 42-43 0.003613320556572183 44-45 0.001991241668946899 46-47 0.0016220788876252833 48-49 4.474700379655954E-5 50-51 4.2509653606731556E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.579760303724763E-4 60-61 5.257772946095745E-4 62-63 0.0 64-65 0.0 66-67 0.0011746088496596878 68-69 7.495123135923723E-4 70-71 0.002035988672743459 72-73 0.0 74-75 0.0 76-77 0.10172112638052898 78-79 0.036815597373619356 80-81 2.237350189827977E-5 82-83 0.0 84-85 0.0 86-87 0.1545225908604692 88-89 0.3367771373238562 90-91 0.2877791681666235 92-93 0.0 94-95 3.3560252847419655E-5 96-97 1.1186750949139885E-5 98-99 1.9017476613537805E-4 100-101 5.145905436604347E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4469573.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.88169329487612 #Duplication Level Percentage of deduplicated Percentage of total 1 67.23663422357836 28.159840927316804 2 15.664192586628534 13.12085819250097 3 6.516777855857954 8.188010742896497 4 3.3242935136965155 5.569081654511341 5 1.9149205942133114 4.010005850544192 6 1.2201229676233982 3.066048954722234 7 0.8227806580236714 2.4121613017812633 8 0.5697222180853891 1.9088744960903035 9 0.4240260522670779 1.5983036163078181 >10 2.0435350437670143 15.25486750285197 >50 0.15606757377783012 4.548837527235775 >100 0.09803922119185812 7.622569533998227 >500 0.006156997139707903 1.7909541077735163 >1k 0.002516337745491353 1.7328109354488912 >5k 1.0707820193579439E-4 0.3840401264451954 >10k+ 1.0707820193579439E-4 0.6327345295749708 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15284 0.341956603013308 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12934 0.28937887355235054 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8778 0.1963945996630998 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8349 0.18679636734873778 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.237350189827977E-5 0.0 0.0 1.1186750949139885E-5 0.0 14-15 2.237350189827977E-5 0.0 0.0 3.3560252847419655E-5 0.0 16-17 2.237350189827977E-5 0.0 0.0 5.5933754745699425E-5 0.0 18-19 2.237350189827977E-5 0.0 0.0 6.712050569483931E-5 2.237350189827977E-5 20-21 2.237350189827977E-5 0.0 0.0 1.2305426044053873E-4 2.237350189827977E-5 22-23 2.237350189827977E-5 0.0 0.0 3.1322902657591675E-4 3.3560252847419655E-5 24-25 2.237350189827977E-5 0.0 0.0 6.264580531518335E-4 4.474700379655954E-5 26-27 2.237350189827977E-5 0.0 0.0 0.001062741340168289 4.474700379655954E-5 28-29 3.3560252847419655E-5 0.0 0.0 0.003937736334097239 4.474700379655954E-5 30-31 4.474700379655954E-5 0.0 0.0 0.013759703667442058 4.474700379655954E-5 32-33 4.474700379655954E-5 0.0 0.0 0.032508698258200505 4.474700379655954E-5 34-35 4.474700379655954E-5 0.0 0.0 0.0618739194996927 4.474700379655954E-5 36-37 4.474700379655954E-5 0.0 0.0 0.11297499783536369 4.474700379655954E-5 38-39 4.474700379655954E-5 0.0 0.0 0.18691942160917835 4.474700379655954E-5 40-41 4.474700379655954E-5 0.0 0.0 0.26732978743159586 4.474700379655954E-5 42-43 4.474700379655954E-5 0.0 0.0 0.3483330510543177 4.474700379655954E-5 44-45 8.949400759311908E-5 0.0 0.0 0.4479063212526118 4.474700379655954E-5 46-47 1.3424101138967862E-4 0.0 0.0 0.574115245460808 4.474700379655954E-5 48-49 1.3424101138967862E-4 0.0 0.0 0.70870304612991 4.474700379655954E-5 50-51 1.3424101138967862E-4 0.0 0.0 0.8502713793912752 4.474700379655954E-5 52-53 1.3424101138967862E-4 0.0 0.0 0.9925444779624362 4.474700379655954E-5 54-55 1.3424101138967862E-4 0.0 0.0 1.1515529559535107 4.474700379655954E-5 56-57 1.3424101138967862E-4 0.0 0.0 1.3446139038337668 4.474700379655954E-5 58-59 1.3424101138967862E-4 0.0 0.0 1.5571845453693227 4.474700379655954E-5 60-61 1.3424101138967862E-4 0.0 0.0 1.792162249056006 4.474700379655954E-5 62-63 1.3424101138967862E-4 0.0 0.0 2.024052409480727 4.474700379655954E-5 64-65 1.3424101138967862E-4 0.0 0.0 2.265261133446081 4.474700379655954E-5 66-67 1.5661451328795838E-4 0.0 0.0 2.5436881778192233 4.474700379655954E-5 68-69 1.5661451328795838E-4 0.0 0.0 2.8618505615637106 4.474700379655954E-5 70-71 1.5661451328795838E-4 0.0 0.0 3.209064937523115 4.474700379655954E-5 72-73 1.5661451328795838E-4 0.0 0.0 3.5564583014977047 4.474700379655954E-5 74-75 1.5661451328795838E-4 0.0 0.0 3.908706715384221 4.474700379655954E-5 76-77 1.5661451328795838E-4 0.0 0.0 4.291819375139415 4.474700379655954E-5 78-79 1.5661451328795838E-4 0.0 0.0 4.722923196466418 4.474700379655954E-5 80-81 1.7898801518623816E-4 0.0 0.0 5.192654868820803 4.474700379655954E-5 82-83 1.7898801518623816E-4 0.0 0.0 5.674445858698359 4.474700379655954E-5 84-85 1.7898801518623816E-4 0.0 0.0 6.150654659852295 4.474700379655954E-5 86-87 1.7898801518623816E-4 0.0 0.0 6.649706806444374 4.474700379655954E-5 88-89 1.7898801518623816E-4 0.0 0.0 7.188415985151154 4.474700379655954E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 20090 0.0 25.395985 1 TACATGG 20395 0.0 24.662045 2 ACATGGG 20040 0.0 24.104437 3 GTATCAA 27785 0.0 23.43763 1 GGTATCA 20970 0.0 22.649168 1 GAGTACT 15815 0.0 20.763863 12-13 CATGGGG 9290 0.0 20.022451 4 AGTACTT 16310 0.0 19.78455 12-13 ATCAACG 32750 0.0 19.661448 3 TCAACGC 32805 0.0 19.642946 4 TATCAAC 33390 0.0 19.497755 2 CAACGCA 33165 0.0 19.444033 5 GTACTTT 16975 0.0 19.358925 14-15 CATGGGA 10995 0.0 19.2912 4 AACGCAG 33760 0.0 19.129456 6 TGTACCG 605 0.0 18.039288 5 AGTACAT 15525 0.0 18.032972 2 AGAGTAC 28545 0.0 17.929216 10-11 GTACCGT 460 0.0 17.536303 6 GAGTACA 18645 0.0 17.527391 1 >>END_MODULE