FastQCFastQC Report
Thu 26 May 2016
SRR938704_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938704_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4484457
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154450.3444118206507499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118440.26411224368970426No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78350.17471457525403855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75880.16920666203288381No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45310.1010378737046648No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG208850.027.151351
TACATGG211550.026.4171832
GTATCAA260150.025.4880051
ACATGGG211350.025.3425773
GGTATCA192250.021.8560351
ATCAACG306150.021.4002343
TCAACGC306650.021.3808124
GAGTACT140850.021.0968212-13
TATCAAC312400.021.0326022
CAACGCA309700.021.032385
TACCTGG33300.020.9424422
CATGGGA120750.020.8232154
AACGCAG314700.020.7123626
CATGGGG96050.020.4979214
AGTACTT148350.019.93425612-13
GTATAGG12700.019.836161
GTACTTT155850.019.00541314-15
ACGCAGA347300.018.6321857
AGTACAT158300.018.5808242
AGAGTAC262250.018.4787810-11
Produced by FastQC (version 0.11.5)