##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938704_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4484457 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.385747259924667 33.0 31.0 34.0 28.0 34.0 2 31.436717310479285 33.0 31.0 34.0 28.0 34.0 3 31.45590402583858 33.0 31.0 34.0 28.0 34.0 4 35.00269664755398 37.0 35.0 37.0 32.0 37.0 5 35.03283563651073 37.0 35.0 37.0 32.0 37.0 6 35.08672376611037 37.0 35.0 37.0 32.0 37.0 7 34.98530279139704 37.0 35.0 37.0 32.0 37.0 8 34.94827177515583 37.0 35.0 37.0 32.0 37.0 9 36.49180290947154 39.0 37.0 39.0 32.0 39.0 10-11 36.362168597000704 39.0 37.0 39.0 32.0 39.0 12-13 36.23769076166858 39.0 37.0 39.0 32.0 39.0 14-15 37.361873689501316 40.0 37.0 41.0 32.0 41.0 16-17 37.24063827571543 40.0 37.0 41.0 31.0 41.0 18-19 37.1678970274439 40.0 37.0 41.0 31.0 41.0 20-21 37.051897810593346 40.0 36.5 41.0 31.0 41.0 22-23 37.07327252775531 40.0 37.0 41.0 31.0 41.0 24-25 36.994969959573694 40.0 37.0 41.0 30.0 41.0 26-27 36.860072133593874 40.0 36.0 41.0 30.0 41.0 28-29 36.67770746826204 39.5 36.0 41.0 30.0 41.0 30-31 36.451150942020405 39.0 36.0 41.0 30.0 41.0 32-33 36.26652557935108 39.0 35.0 41.0 29.5 41.0 34-35 35.964900432761425 39.0 35.0 41.0 28.0 41.0 36-37 35.747244538190465 39.0 35.0 41.0 27.0 41.0 38-39 35.57332415050473 39.0 35.0 40.0 26.5 41.0 40-41 35.45266294224697 39.0 35.0 40.5 26.0 41.0 42-43 35.44228632362848 39.0 35.0 41.0 26.0 41.0 44-45 35.266297458086896 39.0 35.0 41.0 25.0 41.0 46-47 34.96201011627495 39.0 34.5 41.0 24.0 41.0 48-49 34.670509049367624 38.5 34.0 40.5 23.0 41.0 50-51 33.535032558010926 37.5 32.5 39.5 21.5 40.5 52-53 33.6050638906784 37.5 33.0 39.5 22.0 40.5 54-55 34.08659833286393 38.0 33.5 40.0 22.0 41.0 56-57 33.972691119571444 38.0 33.5 40.0 21.0 41.0 58-59 33.75664812930529 38.0 33.0 40.0 20.0 41.0 60-61 33.437289286083015 37.0 33.0 40.0 18.5 41.0 62-63 33.02882946586398 37.0 32.5 40.0 17.5 41.0 64-65 32.62953162445308 36.0 32.0 39.5 13.5 41.0 66-67 32.23268681135754 36.0 31.5 39.0 12.0 41.0 68-69 31.806056452319645 35.0 31.0 39.0 8.0 40.0 70-71 31.378396983179904 35.0 31.0 38.0 7.0 40.0 72-73 30.95886112856027 35.0 30.5 37.0 4.5 39.5 74-75 30.48591758154889 35.0 30.0 37.0 2.0 39.0 76-77 30.02249402770503 34.5 29.5 36.0 2.0 39.0 78-79 29.582966009039666 34.0 29.0 36.0 2.0 38.5 80-81 29.111812868313823 34.0 29.0 35.0 2.0 37.0 82-83 28.69870276379058 34.0 29.0 35.0 2.0 37.0 84-85 28.33922312110474 34.0 28.0 35.0 2.0 36.0 86-87 27.90682060280654 34.0 27.0 35.0 2.0 36.0 88-89 27.44675910595196 34.0 26.0 35.0 2.0 36.0 90-91 26.983455076054916 33.0 25.5 35.0 2.0 35.0 92-93 26.50123593558819 33.0 25.0 35.0 2.0 35.0 94-95 25.966703995600806 33.0 23.5 35.0 2.0 35.0 96-97 25.333990380552205 32.5 19.5 35.0 2.0 35.0 98-99 24.526692194840983 32.0 8.5 35.0 2.0 35.0 100-101 21.987758384125435 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16525892128860775 1101 2 0.2614948306635405 1101 3 0.4183886720181711 1101 4 0.1472295958282004 1101 5 0.09311392323640177 1101 6 0.2767100873271531 1101 7 0.257109413509788 1101 8 0.2841262254424066 1101 9 0.3670353492309957 1101 10-11 0.312285392162174 1101 12-13 0.2861909480954381 1101 14-15 0.3410924707717484 1101 16-17 0.49348741832432097 1101 18-19 0.48762706385941357 1101 20-21 0.5581799900394344 1101 22-23 0.48532543016124663 1101 24-25 0.5617559488802968 1101 26-27 0.623421452169822 1101 28-29 0.6249899589770749 1101 30-31 0.5916532117723605 1101 32-33 0.46133969531647523 1101 34-35 0.4706286397911654 1101 36-37 0.5495760159499028 1101 38-39 0.5993104695404341 1101 40-41 0.6476747890271568 1101 42-43 0.7615168100607903 1101 44-45 0.7499218187181711 1101 46-47 0.6310836167924876 1101 48-49 0.7078852965790574 1101 50-51 0.6766711973568746 1101 52-53 0.7851639441579721 1101 54-55 0.7822592599758096 1101 56-57 0.8061547272206937 1101 58-59 0.7224316400866826 1101 60-61 0.7146516461395933 1101 62-63 0.5422119704767354 1101 64-65 0.47855562329144163 1101 66-67 0.4674931082280196 1101 68-69 0.5357704563907433 1101 70-71 0.5574410787821122 1101 72-73 0.4137840576391305 1101 74-75 0.3588977561500748 1101 76-77 0.3515653225386792 1101 78-79 0.4222574604410596 1101 80-81 0.2740447852741603 1101 82-83 0.33546539883267457 1101 84-85 0.3451745562598525 1101 86-87 0.18341434960561998 1101 88-89 0.19015332054673806 1101 90-91 0.15248981267003003 1101 92-93 0.151359296799491 1101 94-95 -0.03551565614997898 1101 96-97 -0.05601456181791775 1101 98-99 -0.051835217723880334 1101 100-101 0.14480533966453635 1102 1 0.1403745428660521 1102 2 0.16673662809744272 1102 3 0.2441273190841997 1102 4 0.3525270689246156 1102 5 0.30305683597346444 1102 6 0.38640120763660946 1102 7 0.30628933415572845 1102 8 0.3894994945894865 1102 9 0.572139997809181 1102 10-11 0.5340555033900358 1102 12-13 0.4583807885687179 1102 14-15 0.570611088254168 1102 16-17 0.7158588356321189 1102 18-19 0.7382982186254097 1102 20-21 0.6488783911230627 1102 22-23 0.572065598552733 1102 24-25 0.5975409615909015 1102 26-27 0.5870607920802797 1102 28-29 0.6600959705652869 1102 30-31 0.734084041244536 1102 32-33 0.7813932915086141 1102 34-35 0.7610003002492505 1102 36-37 0.7643207500531233 1102 38-39 0.7643004041402364 1102 40-41 0.849718193434505 1102 42-43 0.9197422433916245 1102 44-45 0.8709431768225571 1102 46-47 0.7554249381050226 1102 48-49 0.7099194489794485 1102 50-51 0.7705544506144051 1102 52-53 0.637470924162578 1102 54-55 0.5349108698027152 1102 56-57 0.6310720479445919 1102 58-59 0.5421573007263305 1102 60-61 0.5551241638858713 1102 62-63 0.4347661228553079 1102 64-65 0.40530025505735523 1102 66-67 0.43053136517767854 1102 68-69 0.4592844717682141 1102 70-71 0.3679821640147658 1102 72-73 0.3404960396376744 1102 74-75 0.20190609728555486 1102 76-77 0.2996294342862633 1102 78-79 0.27747676498663765 1102 80-81 0.29387858928144794 1102 82-83 0.237419039931261 1102 84-85 0.20107070510946912 1102 86-87 0.24816084635003932 1102 88-89 0.3184499244205732 1102 90-91 0.19450200618056712 1102 92-93 0.12888299366348477 1102 94-95 0.24275298097931852 1102 96-97 0.19441830170748986 1102 98-99 -0.04720333876868921 1102 100-101 0.2919121265173814 1103 1 0.11048628634556579 1103 2 0.28283019636907625 1103 3 0.3825384874476754 1103 4 0.257616201447334 1103 5 0.2874329024473141 1103 6 0.21530467548442545 1103 7 0.23807700687682143 1103 8 0.33037623803281946 1103 9 0.41196909601698906 1103 10-11 0.3758107083691229 1103 12-13 0.4461762207701696 1103 14-15 0.5111508626315739 1103 16-17 0.5501748079854067 1103 18-19 0.6040721014301837 1103 20-21 0.5626270468159618 1103 22-23 0.5902930239232802 1103 24-25 0.5351163909331049 1103 26-27 0.6019402351875627 1103 28-29 0.5887241427046561 1103 30-31 0.618551786577278 1103 32-33 0.6200761472930978 1103 34-35 0.504294190470091 1103 36-37 0.6363428861631988 1103 38-39 0.5564499685348707 1103 40-41 0.6203477946121652 1103 42-43 0.7288275347016793 1103 44-45 0.6412384735909953 1103 46-47 0.6373050328553234 1103 48-49 0.6270276989717729 1103 50-51 0.7038330598845945 1103 52-53 0.6870889835141156 1103 54-55 0.6731069343638723 1103 56-57 0.6357536786714704 1103 58-59 0.7119409882944083 1103 60-61 0.6251557672034593 1103 62-63 0.5524678481251897 1103 64-65 0.5313022882148744 1103 66-67 0.43566175832064147 1103 68-69 0.3722292812027206 1103 70-71 0.30437565920146525 1103 72-73 0.39290387233986834 1103 74-75 0.4442142356458696 1103 76-77 0.35656833238763497 1103 78-79 0.34178224103709454 1103 80-81 0.48951822325833305 1103 82-83 0.4586402829070799 1103 84-85 0.5830187608292512 1103 86-87 0.47008619430531695 1103 88-89 0.4960370469609501 1103 90-91 0.4257479810821394 1103 92-93 0.28532613849427335 1103 94-95 0.34436409110170985 1103 96-97 0.3673000560744484 1103 98-99 0.4130981409811909 1103 100-101 0.772667584565518 1104 1 0.0013704086106258728 1104 2 0.04451126355602142 1104 3 0.027473723817504236 1104 4 0.09602436329060282 1104 5 0.11296034085798823 1104 6 0.10059504726559965 1104 7 0.1891436905763655 1104 8 0.05130131661630344 1104 9 0.207077177341219 1104 10-11 0.15309088752398026 1104 12-13 0.17884914314672073 1104 14-15 0.31278253448552107 1104 16-17 0.47754673239041523 1104 18-19 0.3380650714987823 1104 20-21 0.4424393174528376 1104 22-23 0.23842656939330453 1104 24-25 0.2974407394256957 1104 26-27 0.25209866026614236 1104 28-29 0.34140962682536724 1104 30-31 0.47396940166530754 1104 32-33 0.39270219055659794 1104 34-35 0.4981490974860847 1104 36-37 0.5134516720290137 1104 38-39 0.4162166762352797 1104 40-41 0.5092367480195108 1104 42-43 0.43484962324165366 1104 44-45 0.3738572972388212 1104 46-47 0.47951297714234187 1104 48-49 0.44891097346427244 1104 50-51 0.5826109705703999 1104 52-53 0.6336487301888312 1104 54-55 0.5644274272332908 1104 56-57 0.5411852297468656 1104 58-59 0.5143768827525292 1104 60-61 0.3731009832886869 1104 62-63 0.44371624161553314 1104 64-65 0.6414606552268438 1104 66-67 0.5530589997693625 1104 68-69 0.6487165607431393 1104 70-71 0.6342051562671678 1104 72-73 0.5839064504975333 1104 74-75 0.5511859068804448 1104 76-77 0.565484848081109 1104 78-79 0.42337154361834806 1104 80-81 0.30206216032960853 1104 82-83 0.42961675044397296 1104 84-85 0.4245929080242483 1104 86-87 0.437911386699362 1104 88-89 0.5567090131739256 1104 90-91 0.47226998445774626 1104 92-93 0.5697996097938791 1104 94-95 0.6340626866638672 1104 96-97 0.5871872907100197 1104 98-99 0.7967031292904458 1104 100-101 1.0706613830511387 1105 1 0.11294779463231563 1105 2 0.1490065227395796 1105 3 0.09308080381523354 1105 4 0.20909212573879188 1105 5 0.1816517903555237 1105 6 0.27174774697603965 1105 7 0.14913418055535743 1105 8 0.17577684605478794 1105 9 0.1995046456884566 1105 10-11 0.24172960608413518 1105 12-13 0.3211247350390778 1105 14-15 0.4183735751940887 1105 16-17 0.38377047337718295 1105 18-19 0.2965772500033026 1105 20-21 0.33826448920098073 1105 22-23 0.3176343988374626 1105 24-25 0.33325554950915404 1105 26-27 0.3984490122788671 1105 28-29 0.4175561521191824 1105 30-31 0.5032801380532064 1105 32-33 0.42698013866310447 1105 34-35 0.5104258958736381 1105 36-37 0.5439554192611737 1105 38-39 0.5489158898842916 1105 40-41 0.5714486085770503 1105 42-43 0.34539368160762507 1105 44-45 0.42722945132954493 1105 46-47 0.5919608139674182 1105 48-49 0.6618222463876791 1105 50-51 0.6643655694462254 1105 52-53 0.6554522276055081 1105 54-55 0.532857351153126 1105 56-57 0.3856850310300075 1105 58-59 0.5232801076432381 1105 60-61 0.5102567773044697 1105 62-63 0.5497636213918398 1105 64-65 0.5256094373763887 1105 66-67 0.5244746444636235 1105 68-69 0.5878637815459626 1105 70-71 0.5073986071273247 1105 72-73 0.4465029701137233 1105 74-75 0.33480950876541726 1105 76-77 0.35547732522866937 1105 78-79 0.379075072261962 1105 80-81 0.5023602356149084 1105 82-83 0.4471687716782853 1105 84-85 0.4559348145705471 1105 86-87 0.48926585498983144 1105 88-89 0.5412243569540856 1105 90-91 0.4580099208683528 1105 92-93 0.4823326109861803 1105 94-95 0.5769741724981969 1105 96-97 0.5789015074817243 1105 98-99 0.5882991805026201 1105 100-101 0.9767030852029599 1106 1 0.23019468344705984 1106 2 0.15581000787119947 1106 3 0.18872864570006698 1106 4 0.15598656993151394 1106 5 0.24917097510164155 1106 6 0.20279016967067776 1106 7 0.22250986747384616 1106 8 0.18171678305073868 1106 9 0.19368388822108784 1106 10-11 0.2279711899452863 1106 12-13 0.31389480779625956 1106 14-15 0.4723012134485245 1106 16-17 0.44374796016285245 1106 18-19 0.4371865759387177 1106 20-21 0.4981670768704731 1106 22-23 0.41319889743445515 1106 24-25 0.2842240891342911 1106 26-27 0.11168688756966105 1106 28-29 0.22668505686206686 1106 30-31 0.09358957003276913 1106 32-33 0.3025402801590431 1106 34-35 0.3617102795566609 1106 36-37 0.4647803942375859 1106 38-39 0.5106258735497349 1106 40-41 0.5607208491293036 1106 42-43 0.674785257299213 1106 44-45 0.7317289133282614 1106 46-47 0.6780439952370116 1106 48-49 0.641849560651373 1106 50-51 0.5625999823978063 1106 52-53 0.46806711067547724 1106 54-55 0.4468106697583494 1106 56-57 0.7032104402061918 1106 58-59 0.910806052303478 1106 60-61 0.8632436341457819 1106 62-63 0.804451807194738 1106 64-65 0.704529695562087 1106 66-67 0.7782909981467228 1106 68-69 0.9052867622332883 1106 70-71 0.7946485257749352 1106 72-73 0.7792671342391309 1106 74-75 0.6314523212192213 1106 76-77 0.7476450241896053 1106 78-79 0.7351439144584759 1106 80-81 0.7472191767569747 1106 82-83 0.6936302060707327 1106 84-85 0.8839325756064405 1106 86-87 0.972597586447776 1106 88-89 1.033545973255503 1106 90-91 1.1885104291205266 1106 92-93 1.281676093801952 1106 94-95 1.3922886503852219 1106 96-97 1.4556449085560814 1106 98-99 1.4932958188527046 1106 100-101 1.990749083741136 1107 1 0.06502964442419312 1107 2 0.07092981208671034 1107 3 0.12294138644200103 1107 4 0.16632136064254155 1107 5 0.10627877649577755 1107 6 -0.04772486582960056 1107 7 -0.004253069245883978 1107 8 0.010932870939384998 1107 9 0.018962280403343357 1107 10-11 0.09479584022640353 1107 12-13 0.1693990982547433 1107 14-15 0.21037967731019336 1107 16-17 0.2206623727924324 1107 18-19 0.2761676562915838 1107 20-21 0.2215903334889333 1107 22-23 0.1592877471162808 1107 24-25 0.1359871060169766 1107 26-27 0.23642229257624336 1107 28-29 0.1663133316445382 1107 30-31 0.08294722843911018 1107 32-33 0.14181860472957197 1107 34-35 0.23187431219095345 1107 36-37 0.21094487193656875 1107 38-39 0.20927165564558692 1107 40-41 0.23701905603528672 1107 42-43 0.15846408073065987 1107 44-45 0.38425094966506634 1107 46-47 0.4442531956166178 1107 48-49 0.34343024575967007 1107 50-51 0.35666182257083534 1107 52-53 0.27869934257599027 1107 54-55 0.2483970853723747 1107 56-57 0.4286788369023 1107 58-59 0.4761631571731897 1107 60-61 0.5216906744243204 1107 62-63 0.4594971441525928 1107 64-65 0.4076626331255966 1107 66-67 0.1959635342376842 1107 68-69 0.30642983550855263 1107 70-71 0.4631675597115361 1107 72-73 0.33093178889210506 1107 74-75 0.38262842621051263 1107 76-77 0.3117906836590407 1107 78-79 0.19714882922601973 1107 80-81 0.2537301718613527 1107 82-83 0.14555314116705276 1107 84-85 0.3961934300591956 1107 86-87 0.46308373305326 1107 88-89 0.5583018495890819 1107 90-91 0.6774862141379678 1107 92-93 0.7675939600346311 1107 94-95 0.7750488705429142 1107 96-97 0.8096828583377942 1107 98-99 1.0689780490720864 1107 100-101 1.3609262172917838 1108 1 0.12861146918285016 1108 2 0.24481710241058252 1108 3 0.08304737328771594 1108 4 0.17732259649738324 1108 5 0.1828538245672675 1108 6 0.13751128588194206 1108 7 0.14588176099994854 1108 8 0.14883010144967557 1108 9 0.1893068852744122 1108 10-11 0.3314304555033303 1108 12-13 0.32388996301818196 1108 14-15 0.37647952448527633 1108 16-17 0.5093026382441579 1108 18-19 0.5072907346666042 1108 20-21 0.5553213058396551 1108 22-23 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-2.404301687219508 2316 56-57 -2.04188181285965 2316 58-59 -1.8775665174145537 2316 60-61 -1.7981709465358762 2316 62-63 -2.108012037415371 2316 64-65 -2.2225692111056574 2316 66-67 -1.9354382851095018 2316 68-69 -2.193889955547583 2316 70-71 -2.148094342479702 2316 72-73 -2.033898478491608 2316 74-75 -2.104129464882295 2316 76-77 -2.1536149848345154 2316 78-79 -2.0139718147776406 2316 80-81 -1.923564406086431 2316 82-83 -1.9640810356880252 2316 84-85 -1.9661756801127623 2316 86-87 -2.0876681872354723 2316 88-89 -2.1634768681961667 2316 90-91 -2.354359088223962 2316 92-93 -2.391196630807624 2316 94-95 -2.5046225681398084 2316 96-97 -2.6145566524812907 2316 98-99 -2.843454556590924 2316 100-101 -2.7347114239664023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25028.0 3 6708.0 4 6459.0 5 7243.0 6 8296.0 7 10284.0 8 12193.0 9 14238.0 10 17145.0 11 20328.0 12 23736.0 13 28216.0 14 29889.0 15 32424.0 16 37085.0 17 43689.0 18 40647.0 19 33054.0 20 34149.0 21 36670.0 22 39762.0 23 44851.0 24 50098.0 25 56834.0 26 65497.0 27 75042.0 28 87144.0 29 102774.0 30 120299.0 31 144128.0 32 175682.0 33 220717.0 34 287911.0 35 395162.0 36 569584.0 37 802893.0 38 678609.0 39 99928.0 40 61.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.97944668044267 18.265363782463744 13.374226050066335 23.38096348702725 2 15.658018622947303 19.90048551008715 39.70092042526529 24.74057544170026 3 19.80205873395615 24.155010346401685 29.123450525520344 26.919480394121827 4 12.765420400628752 15.718525656532895 36.64881691506882 34.867237027769534 5 13.856773284685467 37.37817449344575 33.80245798097199 14.962594240896799 6 32.78067164025694 35.214212360447355 17.317040907437413 14.688075091858286 7 29.396802772352395 32.34723347103143 20.249220208825093 18.00674354779109 8 26.84314164722799 34.40774756865685 19.565991108511835 19.183119675603315 9 26.99199912808721 16.506198085007924 20.12028108467381 36.38152170223106 10-11 25.53066728164808 26.092217714146273 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22.317115845608722 36-37 22.783635074580666 26.131427522934956 28.52460939734631 22.560328005138068 38-39 22.496039441499132 26.34195049083615 28.707495769695957 22.45451429796876 40-41 22.615090772408806 26.245494833008554 28.71553413689487 22.423880257687774 42-43 22.405381704956454 26.456691679589106 28.243900337056076 22.89402627839836 44-45 22.492140947496438 26.476060741227442 28.276213305066676 22.75558500620945 46-47 22.674786244850683 26.666964423338797 27.89328535197245 22.76496397983807 48-49 22.855681930524156 26.663161551725434 27.7262167856118 22.75493973213861 50-51 22.52628380655996 26.861915402141033 27.821511819722378 22.790288971576622 52-53 22.881708199714637 26.886938570570546 27.575966042891952 22.65538718682287 54-55 22.42822518597526 26.616417272367322 27.807310454007666 23.14804708764975 56-57 22.616005789405236 26.968110145538716 27.546200076060927 22.869683988995114 58-59 22.91306351662819 26.563333069641587 27.592088934312 22.931514479418226 60-61 22.674152366724584 26.62335018521862 28.098555677327 22.6039417707298 62-63 22.54936034013708 26.623808140657452 27.984658684839847 22.84217283436562 64-65 22.3690156703757 26.69827719026061 27.87795577467766 23.054751364686027 66-67 22.41263124796762 26.841995408056775 27.560080014118032 23.185293329857576 68-69 22.323541542109414 26.642238047346893 27.868704495192198 23.1655159153515 70-71 22.452985801255654 26.624434561420106 27.727825765130294 23.194753872193942 72-73 22.569504356979515 26.50707217672509 27.758085099021446 23.165338367273954 74-75 22.305528693034894 26.992617742654772 27.48870570497238 23.21314785933795 76-77 22.563961739941128 26.72476889007006 27.475253454642584 23.23601591534623 78-79 22.511983835545884 26.819494354942236 27.506689582873772 23.16183222663811 80-81 22.505559232422424 26.988079675970088 27.433344335951688 23.073016755655797 82-83 22.58119800293665 26.915579062066197 27.393856055930847 23.10936687906631 84-85 22.520291957467965 26.807884145681417 27.27923719322229 23.392586703628336 86-87 22.51674762082314 26.70166315989074 27.490220410297823 23.291368808988302 88-89 22.631762716004523 26.67582163242958 27.227179389527894 23.465236262038005 90-91 22.575952814123873 26.759930344488424 27.36157803414876 23.302538807238943 92-93 22.763083994109447 26.643393194334465 27.36598457134053 23.227538240215555 94-95 22.622884438734403 26.509357086170702 27.3634818602348 23.504276614860096 96-97 22.62593873991384 26.325411621158928 27.45520296849202 23.593446670435213 98-99 22.611830467596707 26.442840936360234 27.12522707852082 23.82010151752224 100-101 22.915001946728815 25.80024659311263 27.611779063855167 23.672972396303386 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4493.0 1 3020.0 2 1516.0 3 1574.5 4 1494.5 5 1479.5 6 2132.5 7 3535.5 8 5330.5 9 6106.0 10 5459.0 11 4732.0 12 4699.5 13 4988.0 14 5308.0 15 5616.5 16 5965.5 17 6436.5 18 6817.5 19 7271.0 20 8008.0 21 9178.0 22 10859.5 23 12921.5 24 15089.0 25 17988.5 26 22428.0 27 27841.5 28 32689.5 29 38040.0 30 45340.0 31 53943.0 32 63630.0 33 71987.0 34 80152.5 35 88080.5 36 96852.0 37 106453.5 38 112952.5 39 116698.0 40 119588.0 41 126561.0 42 133578.5 43 139902.0 44 148583.5 45 156065.5 46 159652.0 47 166789.0 48 186948.0 49 189439.5 50 173965.0 51 165821.5 52 159822.5 53 158643.5 54 157505.5 55 148695.0 56 135492.5 57 121979.0 58 110882.5 59 98309.5 60 83676.5 61 69756.0 62 57423.0 63 45848.5 64 35428.5 65 28089.5 66 21685.0 67 17369.5 68 13028.5 69 8648.0 70 6384.0 71 4980.5 72 3911.5 73 3174.5 74 2606.5 75 2145.0 76 1607.0 77 1004.0 78 642.0 79 448.0 80 336.0 81 277.5 82 208.0 83 183.0 84 149.0 85 98.5 86 71.5 87 43.5 88 34.0 89 28.0 90 18.5 91 13.5 92 12.5 93 11.0 94 9.0 95 6.5 96 4.5 97 4.5 98 3.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2771796005625653 2 0.17219476070346978 3 0.20042560336736423 4 0.17045541968626302 5 0.2441321212356368 6 0.20258863001696747 7 0.248502773022464 8 0.254545868095067 9 0.15486824826283316 10-11 0.2124002972935185 12-13 0.21887822762042317 14-15 0.17958696002659855 16-17 0.29165180979547806 18-19 0.23907019289068887 20-21 0.20487430250752764 22-23 0.25152432055876556 24-25 0.23944928003546473 26-27 0.3492061580699737 28-29 0.3211760086003723 30-31 0.2967583366280466 32-33 0.19831832482728678 34-35 0.3673019944220672 36-37 0.4055228983129954 38-39 0.38542013001797093 40-41 0.34698738331084455 42-43 0.3813059195349626 44-45 0.3896681359638413 46-47 0.39519834842880647 48-49 0.41482168298190836 50-51 0.4071061446235297 52-53 0.4066155612597021 54-55 0.3999480873604095 56-57 0.37877495536248873 58-59 0.3535656602348958 60-61 0.3196373607774587 62-63 0.38877616621142763 64-65 0.4228159618879164 66-67 0.42914894713005386 68-69 0.434288922828338 70-71 0.43370914248926906 72-73 0.42447725555178695 74-75 0.41961602040113216 76-77 0.4030811311157627 78-79 0.3763331881652561 80-81 0.38663543880563467 82-83 0.39689309095839254 84-85 0.39798575390509927 86-87 0.3931022195106342 88-89 0.40202191703477147 90-91 0.424867492318468 92-93 0.42370793164033016 94-95 0.43301786593114844 96-97 0.433252007991157 98-99 0.43074334306249346 100-101 0.4011633961480732 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4484457.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.7320548505286 #Duplication Level Percentage of deduplicated Percentage of total 1 70.20180355289133 31.402709271339692 2 14.452038870865755 12.929387909470766 3 5.859745196483533 7.863553306176686 4 3.029630020543811 5.420863050230953 5 1.7348627933982377 3.880198881621562 6 1.0668357388071203 2.8633052870894584 7 0.7258000511216333 2.2726569388102558 8 0.5016178723158706 1.7950718542751183 9 0.3827184441269009 1.5407804191494148 >10 1.813688029333497 14.426252590846534 >50 0.1367950543316094 4.253948460854884 >100 0.08675502588947358 7.145025851119022 >500 0.005206314506326141 1.5847657913917412 >1k 0.0023027925541829353 1.665022745642546 >5k 1.0012141539925276E-4 0.34537006490631433 >10k+ 1.0012141539925276E-4 0.6110875770750446 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15445 0.3444118206507499 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11844 0.26411224368970426 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7835 0.17471457525403855 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7588 0.16920666203288381 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4531 0.1010378737046648 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.229924381034315E-5 0.0 12-13 0.0 0.0 0.0 6.689773143102944E-5 0.0 14-15 0.0 0.0 0.0 1.226458409568873E-4 0.0 16-17 0.0 0.0 0.0 1.4494508476723046E-4 0.0 18-19 0.0 0.0 0.0 1.783939504827452E-4 2.229924381034315E-5 20-21 0.0 0.0 0.0 2.6759092572411775E-4 5.574810952585787E-5 22-23 0.0 0.0 0.0 4.6828412001720604E-4 7.804735333620102E-5 24-25 2.229924381034315E-5 0.0 0.0 7.470246676464954E-4 8.91969752413726E-5 26-27 2.229924381034315E-5 1.1149621905171574E-5 0.0 0.001226458409568873 8.91969752413726E-5 28-29 2.229924381034315E-5 2.229924381034315E-5 0.0 0.0038912180449048794 8.91969752413726E-5 30-31 2.229924381034315E-5 2.229924381034315E-5 0.0 0.013502192127162777 8.91969752413726E-5 32-33 2.229924381034315E-5 2.229924381034315E-5 0.0 0.031475382638299354 8.91969752413726E-5 34-35 2.229924381034315E-5 2.229924381034315E-5 0.0 0.05892575176883176 8.91969752413726E-5 36-37 2.229924381034315E-5 2.229924381034315E-5 0.0 0.10732626045918156 8.91969752413726E-5 38-39 4.45984876206863E-5 2.229924381034315E-5 0.0 0.17661001097791773 8.91969752413726E-5 40-41 4.45984876206863E-5 2.229924381034315E-5 0.0 0.25261698350547235 8.91969752413726E-5 42-43 7.804735333620102E-5 2.229924381034315E-5 0.0 0.331745850166475 8.91969752413726E-5 44-45 1.3379546286205888E-4 2.229924381034315E-5 0.0 0.42940538843387277 8.91969752413726E-5 46-47 1.5609470667240203E-4 2.229924381034315E-5 0.0 0.5503453372392688 8.91969752413726E-5 48-49 1.5609470667240203E-4 2.229924381034315E-5 0.0 0.6801715347030867 8.91969752413726E-5 50-51 1.5609470667240203E-4 2.229924381034315E-5 0.0 0.8178805148538608 8.91969752413726E-5 52-53 1.5609470667240203E-4 2.229924381034315E-5 0.0 0.9586667906504622 8.91969752413726E-5 54-55 1.5609470667240203E-4 2.229924381034315E-5 0.0 1.1124758248323041 1.1149621905171574E-4 56-57 1.5609470667240203E-4 2.229924381034315E-5 0.0 1.2965337832428765 1.1149621905171574E-4 58-59 1.5609470667240203E-4 2.229924381034315E-5 0.0 1.5050428624914902 1.1149621905171574E-4 60-61 1.5609470667240203E-4 2.229924381034315E-5 0.0 1.734502081299921 1.1149621905171574E-4 62-63 1.5609470667240203E-4 2.229924381034315E-5 0.0 1.9606164135368005 1.1149621905171574E-4 64-65 1.5609470667240203E-4 2.229924381034315E-5 0.0 2.1929968332843863 1.1149621905171574E-4 66-67 1.672443285775736E-4 2.229924381034315E-5 0.0 2.4633863141067023 1.1149621905171574E-4 68-69 2.0069319429308832E-4 2.229924381034315E-5 0.0 2.7711716267989637 1.1149621905171574E-4 70-71 2.0069319429308832E-4 2.229924381034315E-5 0.0 3.111703379026714 1.1149621905171574E-4 72-73 2.0069319429308832E-4 2.229924381034315E-5 0.0 3.4480540230400245 1.1149621905171574E-4 74-75 2.0069319429308832E-4 2.229924381034315E-5 0.0 3.7945173741213263 1.1149621905171574E-4 76-77 2.118428161982599E-4 3.344886571551472E-5 0.0 4.167717518531228 1.1149621905171574E-4 78-79 2.2299243810343147E-4 4.45984876206863E-5 0.0 4.588649194317172 1.1149621905171574E-4 80-81 2.2299243810343147E-4 4.45984876206863E-5 0.0 5.048481900930257 1.1149621905171574E-4 82-83 2.452916819137746E-4 4.45984876206863E-5 0.0 5.512116628613008 1.1149621905171574E-4 84-85 2.452916819137746E-4 4.45984876206863E-5 0.0 5.98112547405405 1.1149621905171574E-4 86-87 2.452916819137746E-4 4.45984876206863E-5 0.0 6.4655876954556595 1.1149621905171574E-4 88-89 2.452916819137746E-4 4.45984876206863E-5 0.0 6.998483874413335 1.1149621905171574E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 20885 0.0 27.15135 1 TACATGG 21155 0.0 26.417183 2 GTATCAA 26015 0.0 25.488005 1 ACATGGG 21135 0.0 25.342577 3 GGTATCA 19225 0.0 21.856035 1 ATCAACG 30615 0.0 21.400234 3 TCAACGC 30665 0.0 21.380812 4 GAGTACT 14085 0.0 21.09682 12-13 TATCAAC 31240 0.0 21.032602 2 CAACGCA 30970 0.0 21.03238 5 TACCTGG 3330 0.0 20.942442 2 CATGGGA 12075 0.0 20.823215 4 AACGCAG 31470 0.0 20.712362 6 CATGGGG 9605 0.0 20.497921 4 AGTACTT 14835 0.0 19.934256 12-13 GTATAGG 1270 0.0 19.83616 1 GTACTTT 15585 0.0 19.005413 14-15 ACGCAGA 34730 0.0 18.632185 7 AGTACAT 15830 0.0 18.580824 2 AGAGTAC 26225 0.0 18.47878 10-11 >>END_MODULE