FastQCFastQC Report
Thu 26 May 2016
SRR938704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938704_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4484457
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151970.3388816081857848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128610.2867905746448232No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT89750.20013571319782977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85800.1913275118927442No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG210250.027.1077921
TACATGG211100.026.438992
ACATGGG209050.025.4723193
GTATCAA284300.023.3604871
GGTATCA215250.023.2070241
GAGTACT153050.022.04050812-13
CATGGGA115050.021.6157574
GTACTTT167400.020.03776614-15
CATGGGG92900.019.9239144
TCAACGC332500.019.7832374
ATCAACG332550.019.7659913
CAACGCA335950.019.5942025
TATCAAC339150.019.4233172
AGTACTT161450.019.35098512-13
AACGCAG341300.019.2816816
GTATAGG11500.019.029761
TACCTGG33500.018.2755582
AGAGTAC282850.017.93109710-11
CGTATAG2152.3649107E-517.7021031
ATGGGAG54400.017.5356945
Produced by FastQC (version 0.11.5)