##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938704_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4484457 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08971342572802 33.0 31.0 34.0 30.0 34.0 2 32.2928229660804 34.0 31.0 34.0 30.0 34.0 3 32.3792367727018 34.0 31.0 34.0 30.0 34.0 4 35.98345240014566 37.0 35.0 37.0 35.0 37.0 5 35.88907107371082 37.0 35.0 37.0 35.0 37.0 6 35.91621995706504 37.0 35.0 37.0 35.0 37.0 7 35.8334244257443 37.0 35.0 37.0 35.0 37.0 8 35.81768561946296 37.0 35.0 37.0 33.0 37.0 9 37.47473350731203 39.0 37.0 39.0 34.0 39.0 10-11 37.40032695151275 39.0 37.0 39.0 34.0 39.0 12-13 37.32169781090553 39.0 37.0 39.0 34.0 39.0 14-15 38.55695695153281 40.0 38.0 41.0 34.0 41.0 16-17 38.48479637557011 40.0 38.0 41.0 34.0 41.0 18-19 38.62184039227046 40.0 38.0 41.0 34.0 41.0 20-21 38.622087021907 40.0 38.0 41.0 34.0 41.0 22-23 38.54773498775883 40.0 38.0 41.0 34.0 41.0 24-25 38.47006192722999 40.0 38.0 41.0 34.0 41.0 26-27 38.2998580430139 40.0 38.0 41.0 33.5 41.0 28-29 38.13580986505167 40.0 38.0 41.0 33.0 41.0 30-31 37.92307719752915 40.0 37.0 41.0 33.0 41.0 32-33 37.7782057002665 40.0 37.0 41.0 33.0 41.0 34-35 37.745504639692165 40.0 37.0 41.0 33.0 41.0 36-37 37.63296559650365 40.0 37.0 41.0 32.5 41.0 38-39 37.510749461974996 40.0 37.0 41.0 32.0 41.0 40-41 37.339233378756894 40.0 36.5 41.0 31.5 41.0 42-43 37.141952414751664 40.0 36.0 41.0 31.0 41.0 44-45 36.904315840245545 40.0 36.0 41.0 31.0 41.0 46-47 36.600192174883155 39.5 36.0 41.0 30.0 41.0 48-49 36.30368414726688 39.0 35.0 41.0 30.0 41.0 50-51 36.0642165818515 39.0 35.0 41.0 29.0 41.0 52-53 35.83794849632854 39.0 35.0 40.5 28.5 41.0 54-55 35.561680823341604 38.5 35.0 40.0 28.0 41.0 56-57 35.28552921791869 38.0 34.5 40.0 27.5 41.0 58-59 34.97707637736296 38.0 34.0 40.0 27.0 41.0 60-61 34.60853788987161 37.5 34.0 40.0 26.0 41.0 62-63 34.25039051550723 37.0 33.5 40.0 26.0 41.0 64-65 33.85170924818768 36.5 33.0 39.0 25.5 41.0 66-67 33.4443365161044 36.0 33.0 39.0 25.0 40.0 68-69 33.10755237479142 35.5 32.5 39.0 23.5 40.0 70-71 32.67353461076782 35.0 32.0 38.0 23.0 40.0 72-73 32.19385145180342 35.0 31.5 37.0 22.0 39.5 74-75 31.708664170489314 35.0 31.0 37.0 20.5 39.0 76-77 29.775533135895827 33.0 28.5 35.0 18.0 37.5 78-79 30.718153502196586 34.0 30.5 36.0 18.0 38.0 80-81 30.7701020435696 34.0 31.0 35.5 18.0 37.0 82-83 30.460186484116136 34.0 30.5 35.0 17.5 37.0 84-85 30.06748330957349 34.0 30.0 35.0 11.5 36.5 86-87 29.494794464524915 34.0 29.5 35.0 6.0 36.0 88-89 28.902387067152166 34.0 29.0 35.0 2.0 35.5 90-91 28.393425335553445 34.0 28.5 35.0 2.0 35.0 92-93 28.098124366004626 33.0 28.0 35.0 2.0 35.0 94-95 27.757734994448604 33.0 27.0 35.0 2.0 35.0 96-97 27.148387084545575 33.0 26.5 35.0 2.0 35.0 98-99 26.312784134177228 33.0 25.0 35.0 2.0 35.0 100-101 22.621480036490482 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15686207311201628 1101 2 0.21179265375749878 1101 3 0.08499269790901565 1101 4 0.11384384595443464 1101 5 0.03467880626771347 1101 6 0.09053675899269109 1101 7 0.20913653167087176 1101 8 0.20484502899003587 1101 9 0.1922120920015402 1101 10-11 0.31084470146277 1101 12-13 0.24552941232577297 1101 14-15 0.3799114293406589 1101 16-17 0.3890702968299422 1101 18-19 0.4171363413506981 1101 20-21 0.31903493790030524 1101 22-23 0.23003978810807268 1101 24-25 0.2614027384272788 1101 26-27 0.12118616569787832 1101 28-29 0.25277388536229495 1101 30-31 0.28041409303013864 1101 32-33 0.34168518831204864 1101 34-35 0.3680495582096057 1101 36-37 0.3795623593388484 1101 38-39 0.4562080343601522 1101 40-41 0.43806482756595955 1101 42-43 0.4900971021628209 1101 44-45 0.49615315139824645 1101 46-47 0.4618037894773934 1101 48-49 0.39557033819180276 1101 50-51 0.4467384066889224 1101 52-53 0.31626805186143514 1101 54-55 0.2580281557682156 1101 56-57 0.41543002333298773 1101 58-59 0.4711769310026668 1101 60-61 0.5598063044198796 1101 62-63 0.348047417167912 1101 64-65 0.22813730678974053 1101 66-67 0.29846499299068086 1101 68-69 0.20403229325622618 1101 70-71 0.3493942582956109 1101 72-73 0.18128267014568422 1101 74-75 0.06430356735244303 1101 76-77 0.06364856284848841 1101 78-79 0.030364368702826994 1101 80-81 0.15537738782301247 1101 82-83 0.18489525186366862 1101 84-85 0.2263716355999641 1101 86-87 0.3375936140100073 1101 88-89 0.3620907565571443 1101 90-91 0.3507174681497425 1101 92-93 0.4317162890337265 1101 94-95 0.40907861092163245 1101 96-97 0.30146177814270203 1101 98-99 0.14684284657954905 1101 100-101 0.7130231544346408 1102 1 0.32206032661472506 1102 2 0.2092101736772065 1102 3 0.23235773276989846 1102 4 0.16554848361465702 1102 5 -0.05621502239076648 1102 6 0.055360985486977654 1102 7 0.11821399501648955 1102 8 0.16747867177639364 1102 9 0.21989031870219122 1102 10-11 0.18976139061075514 1102 12-13 0.2117577523535914 1102 14-15 0.3248861591148682 1102 16-17 0.3346493323969639 1102 18-19 0.2815994470392127 1102 20-21 0.27090114852467195 1102 22-23 0.22801366799669864 1102 24-25 0.2896375482159854 1102 26-27 0.24111570269046467 1102 28-29 0.19765279786176393 1102 30-31 0.24384300680138438 1102 32-33 0.2575651327623163 1102 34-35 0.30819165138544236 1102 36-37 0.2963568691403964 1102 38-39 0.3459986826635557 1102 40-41 0.3893989486255478 1102 42-43 0.4239667957265283 1102 44-45 0.25888518476675415 1102 46-47 0.20228702685874822 1102 48-49 0.20337553654049856 1102 50-51 0.19332296088472845 1102 52-53 0.07735621778591906 1102 54-55 0.12529009272893177 1102 56-57 0.12891324182358943 1102 58-59 0.08568767262737964 1102 60-61 0.1583072809627737 1102 62-63 0.14372266914502774 1102 64-65 0.11040955904407213 1102 66-67 0.2527175619720552 1102 68-69 0.2451945763838026 1102 70-71 0.26421899796391557 1102 72-73 0.266235133621457 1102 74-75 0.18656631076488495 1102 76-77 0.1441921425531163 1102 78-79 0.0031338429503442455 1102 80-81 0.24633530125645464 1102 82-83 0.3031681540392661 1102 84-85 0.4359553265230396 1102 86-87 0.37052540396048883 1102 88-89 0.24057283441312904 1102 90-91 0.2860712014808193 1102 92-93 0.3675434811780285 1102 94-95 0.5464596097090038 1102 96-97 0.43000480471019387 1102 98-99 0.5593892919272356 1102 100-101 1.199421142356158 1103 1 0.16890773947262971 1103 2 0.16447635674860805 1103 3 0.1185353809839711 1103 4 0.005797081907438439 1103 5 -0.012319521133150602 1103 6 0.009541308723036934 1103 7 0.03033224749452046 1103 8 0.08385206322825667 1103 9 0.13592771894379752 1103 10-11 0.17917101806925473 1103 12-13 0.1913845204877731 1103 14-15 0.24533693982505866 1103 16-17 0.17128859205070057 1103 18-19 0.020879850870137773 1103 20-21 0.12685432899048266 1103 22-23 0.1410460444069841 1103 24-25 0.2377135829486292 1103 26-27 0.27441344859623484 1103 28-29 0.19828774399854154 1103 30-31 0.32152901988627036 1103 32-33 0.3237499496363583 1103 34-35 0.21893447651610387 1103 36-37 0.23588255828907023 1103 38-39 0.17253529974055226 1103 40-41 0.2059821927947354 1103 42-43 0.2687940320971336 1103 44-45 0.2203578422597019 1103 46-47 0.11570660967031898 1103 48-49 0.06091910147764423 1103 50-51 0.1054139556357967 1103 52-53 0.06835586301987462 1103 54-55 -0.0235977235609397 1103 56-57 -0.05018652566188564 1103 58-59 0.04312278848079387 1103 60-61 0.09270467935818516 1103 62-63 0.0853352489844994 1103 64-65 0.03231900706605728 1103 66-67 0.08946453242849373 1103 68-69 0.004415022016097225 1103 70-71 -0.004595776418376829 1103 72-73 0.03658235452628844 1103 74-75 0.10754968056897596 1103 76-77 0.15572646362498688 1103 78-79 0.033385030604954125 1103 80-81 0.2143547758389559 1103 82-83 0.2199476733997301 1103 84-85 0.2651345007786823 1103 86-87 0.20718326731845949 1103 88-89 0.1786685563141006 1103 90-91 0.2543789968093684 1103 92-93 0.27979134842590625 1103 94-95 0.3533738658473453 1103 96-97 0.3480884168464726 1103 98-99 0.37768510363447305 1103 100-101 1.0629248109773002 1104 1 0.037629190054069284 1104 2 0.05305271821714541 1104 3 0.033170103270713014 1104 4 0.053617648327900724 1104 5 -0.08817581013455822 1104 6 0.0020394893024686667 1104 7 0.0021571650399323516 1104 8 0.023840604749750582 1104 9 0.053565646507635734 1104 10-11 0.07563059983478837 1104 12-13 0.026328621694013066 1104 14-15 0.040983782549695036 1104 16-17 0.13317782804890044 1104 18-19 0.15068050818420176 1104 20-21 0.09538696629866905 1104 22-23 0.11070120614186152 1104 24-25 0.07162032409541297 1104 26-27 0.07846049803801947 1104 28-29 0.10623135357410973 1104 30-31 -0.07863008263614546 1104 32-33 -0.01789503882774568 1104 34-35 0.03127411588371842 1104 36-37 0.1004800211651471 1104 38-39 0.0534214156260262 1104 40-41 0.2052461016843452 1104 42-43 0.16819672019424559 1104 44-45 0.1852185404526665 1104 46-47 0.11680716556136872 1104 48-49 0.09372306084650717 1104 50-51 -0.007744326211799546 1104 52-53 0.09237685990750322 1104 54-55 0.08980763172932171 1104 56-57 -0.0521690166422033 1104 58-59 0.09808390320493032 1104 60-61 0.14928585261348104 1104 62-63 0.13937416910032852 1104 64-65 0.013253511796548878 1104 66-67 0.030117743125131824 1104 68-69 0.09835122229171844 1104 70-71 -0.053424379258217414 1104 72-73 -0.07011020985023464 1104 74-75 -0.05429077947345107 1104 76-77 0.09159353366106515 1104 78-79 -0.03467058946906221 1104 80-81 0.0462085718470675 1104 82-83 0.11177508780954426 1104 84-85 0.13630713669326866 1104 86-87 0.13796505685408178 1104 88-89 0.15196643030180468 1104 90-91 0.21754550476062562 1104 92-93 0.32176721981222656 1104 94-95 0.2361521791283394 1104 96-97 0.36030814865614147 1104 98-99 0.7573034317805245 1104 100-101 1.6373970822335515 1105 1 0.06662765709545937 1105 2 0.02808820337016016 1105 3 0.04076250660915548 1105 4 0.004983117015804339 1105 5 -0.008136918406854932 1105 6 -0.029166154256266452 1105 7 0.07066764025709915 1105 8 0.03199367350578797 1105 9 0.013891826034118537 1105 10-11 0.09918670895873305 1105 12-13 0.07101578458505742 1105 14-15 0.06431065758518884 1105 16-17 0.05810579318828957 1105 18-19 0.09133194462319949 1105 20-21 0.026518445887610653 1105 22-23 -0.032438804077862926 1105 24-25 0.018298558921955532 1105 26-27 9.21205607085085E-6 1105 28-29 -3.0252764998550674E-4 1105 30-31 -0.0040544850661703435 1105 32-33 0.0058402703189059935 1105 34-35 0.10005392341224706 1105 36-37 0.07628659869310894 1105 38-39 0.15671692178624852 1105 40-41 0.08271799744316155 1105 42-43 0.12306244437525038 1105 44-45 0.15324251422204327 1105 46-47 0.03519273303994197 1105 48-49 0.13769413334976832 1105 50-51 0.06437002114526535 1105 52-53 0.15691867561181994 1105 54-55 0.21091242648319053 1105 56-57 0.22793694202469084 1105 58-59 0.24231863039508283 1105 60-61 0.2810468388168559 1105 62-63 0.22012489299744686 1105 64-65 0.11274217074164739 1105 66-67 0.011659785934426736 1105 68-69 0.024352258458186782 1105 70-71 0.1196466478240481 1105 72-73 0.11026579083108601 1105 74-75 -0.03885367335864842 1105 76-77 0.09935449147631559 1105 78-79 -0.13155073481624768 1105 80-81 -0.021366514736705255 1105 82-83 0.13896061838354967 1105 84-85 0.09884049273710005 1105 86-87 0.12949865419695428 1105 88-89 0.11196756582670275 1105 90-91 0.008659667594894671 1105 92-93 -2.0767089350215429E-4 1105 94-95 0.25639681377320755 1105 96-97 0.3640489934866622 1105 98-99 0.4477277632402412 1105 100-101 1.2092619689649453 1106 1 0.16835699599233322 1106 2 0.09105746704296536 1106 3 0.0596200582387425 1106 4 0.10410034637552457 1106 5 0.011040596388752988 1106 6 0.06737882937942885 1106 7 0.11346558564596165 1106 8 0.13545587542537874 1106 9 0.07521243956154677 1106 10-11 0.10265680583557923 1106 12-13 0.14466054080022417 1106 14-15 0.12229332895496015 1106 16-17 0.2066931221709254 1106 18-19 0.10627261344736638 1106 20-21 0.12437392377508871 1106 22-23 0.13273653949007524 1106 24-25 0.14002495494491285 1106 26-27 0.15295705178981223 1106 28-29 0.2184295742530864 1106 30-31 0.21706024628331022 1106 32-33 0.23961599586547777 1106 34-35 0.15152822086403717 1106 36-37 0.14263240972032776 1106 38-39 0.17947515439595207 1106 40-41 0.3340968002381146 1106 42-43 0.2900171282359878 1106 44-45 0.28961348384636665 1106 46-47 0.3390032742037832 1106 48-49 0.2817632583916989 1106 50-51 0.3230080426186319 1106 52-53 0.29464613619538227 1106 54-55 0.41161329273163005 1106 56-57 0.3238565306264718 1106 58-59 0.3552351008320116 1106 60-61 0.337945658417766 1106 62-63 0.3612609155591784 1106 64-65 0.4159419854733173 1106 66-67 0.3364333837328104 1106 68-69 0.27510211920945693 1106 70-71 0.2783317186658465 1106 72-73 0.3615998326510166 1106 74-75 0.5396771231532931 1106 76-77 0.5194515285103023 1106 78-79 0.41741800069535984 1106 80-81 0.3384842865900133 1106 82-83 0.4358502111822524 1106 84-85 0.5270260851037527 1106 86-87 0.6647823852301791 1106 88-89 0.7453951123165581 1106 90-91 0.6477827506649483 1106 92-93 0.7221788808592038 1106 94-95 0.7881530779068946 1106 96-97 0.8880105320010117 1106 98-99 1.0885224490415375 1106 100-101 1.6122689179003586 1107 1 0.05986473464866293 1107 2 0.03420452886014402 1107 3 0.026019872370632413 1107 4 -0.030648945602401056 1107 5 -0.11144623787741637 1107 6 -0.036164812750442366 1107 7 -0.0069272091432068805 1107 8 0.018488420575216935 1107 9 -0.006185477685924923 1107 10-11 -0.08251101919334758 1107 12-13 0.05160573966708881 1107 14-15 0.00331740285071902 1107 16-17 0.022310252745924686 1107 18-19 0.04284138908978008 1107 20-21 0.0076604938608184625 1107 22-23 0.0288103826677073 1107 24-25 0.005997656299818743 1107 26-27 0.025331176948640177 1107 28-29 0.06126136206002286 1107 30-31 0.0870122135599729 1107 32-33 0.05879087266877292 1107 34-35 0.0245812574961235 1107 36-37 0.022718004511553147 1107 38-39 -0.05614310853798088 1107 40-41 -0.2154309621152919 1107 42-43 -0.13278654665350587 1107 44-45 -0.03206593544773284 1107 46-47 -0.18966684444745852 1107 48-49 -0.10321145180090951 1107 50-51 -0.21718433197010256 1107 52-53 -0.21750079525393318 1107 54-55 -0.19015991629751738 1107 56-57 -0.16925195219854317 1107 58-59 -0.19129784242993964 1107 60-61 -0.07468430748228627 1107 62-63 -0.2266667091155128 1107 64-65 -0.11304397519992904 1107 66-67 -0.13402171370090343 1107 68-69 -0.1367296069407189 1107 70-71 -0.2461139957633165 1107 72-73 -0.16856688246461005 1107 74-75 -0.19244921878594212 1107 76-77 -0.25628089287710054 1107 78-79 -0.21805733857027576 1107 80-81 -0.06994761434287255 1107 82-83 -0.14555061483510556 1107 84-85 -0.19759975318311618 1107 86-87 -0.14688534584777102 1107 88-89 -0.07317004509095071 1107 90-91 0.06742761597149638 1107 92-93 0.21949958313794937 1107 94-95 0.2481084321827005 1107 96-97 0.41794740848786205 1107 98-99 0.6410183705796904 1107 100-101 1.424858843552201 1108 1 0.14298292091587683 1108 2 0.06654933103632743 1108 3 0.040211841279095495 1108 4 0.08320399042075621 1108 5 0.009590263465284465 1108 6 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-2.2648532003354482 2316 92-93 -1.9102294385002203 2316 94-95 -1.7101301934698228 2316 96-97 -1.8478528789127822 2316 98-99 -1.977726852262915 2316 100-101 -1.8819481558616857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 27.0 4 48.0 5 143.0 6 333.0 7 727.0 8 1653.0 9 2997.0 10 4851.0 11 7483.0 12 10373.0 13 14498.0 14 17687.0 15 22672.0 16 28781.0 17 37779.0 18 36521.0 19 26658.0 20 25649.0 21 27036.0 22 28938.0 23 32043.0 24 37409.0 25 44585.0 26 53641.0 27 65224.0 28 79750.0 29 96897.0 30 117304.0 31 141578.0 32 174962.0 33 220865.0 34 291241.0 35 410210.0 36 615336.0 37 945918.0 38 787000.0 39 75619.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.37068485134459 19.206321780373433 14.038771935196968 23.384221433085006 2 16.127415203223045 20.661721140374407 38.872889181454966 24.33797447494758 3 19.8325237592868 24.25807628437512 29.410182771292043 26.499217185046035 4 13.180324842004282 16.3370949927717 36.20467316332836 34.277907001895656 5 13.78997369010987 37.604628934633475 33.814822539289175 14.790574835967481 6 32.84532311210936 35.68081629449114 16.88216840043316 14.591692192966338 7 29.30704876866921 32.49873953524362 20.115657257946726 18.078554438140447 8 26.871703753654007 34.81739260739929 19.248595760869154 19.062307878077547 9 26.738755662056747 16.50456231378738 19.99644550053663 36.76023652361925 10-11 25.56141133698015 26.32862796989691 26.72823041897826 21.381730274144672 12-13 26.35315713808829 23.63961790691716 27.42306370648665 22.584161248507904 14-15 22.926599586081437 25.15932252221395 25.907194561125234 26.006883330579377 16-17 22.53349920004656 28.463367997424804 26.464649573118443 22.53848322941019 18-19 22.326616132120343 26.90753863845723 28.805616822727924 21.9602284066945 20-21 23.116154308091257 25.940688025328374 28.776962294431634 22.166195372148735 22-23 22.69807693551304 26.022871888391393 28.7089830496758 22.570068126419766 24-25 22.687110103082027 26.20280108646824 28.838047394339334 22.2720414161104 26-27 22.508972762272812 26.308596123677503 28.900523034828478 22.281908079221214 28-29 22.10249758220449 26.697647006092378 28.92806197049052 22.271793441212616 30-31 22.360399486493012 26.235684721695403 28.875402306232395 22.52851348557919 32-33 22.15678509126077 26.460895934557964 28.80767950278038 22.574639471400886 34-35 22.83520046742467 26.366799021887115 28.553380856328243 22.244619654359973 36-37 22.78929543601873 26.181062717245485 28.597696765725587 22.431945081010195 38-39 22.451327234290535 26.427443417777447 28.767719335494114 22.35351001243791 40-41 22.555023982132294 26.32487133612507 28.781101087349825 22.339003594392814 42-43 22.418209635466756 26.49228730245757 28.31358759215692 22.775915469918758 44-45 22.502785826215735 26.49075988775969 28.293524506685202 22.712929779339373 46-47 22.66817380713114 26.79583282648711 27.883284176218137 22.652709190163613 48-49 22.89438823528231 26.75433222099364 27.700391457793106 22.650888085930944 50-51 22.53641746878941 26.94670812841248 27.74527785427661 22.771596548521504 52-53 22.91032113809989 26.946874504538677 27.49283804036921 22.649966316992224 54-55 22.458872947159488 26.688571213861568 27.711649370258208 23.14090646872074 56-57 22.622413254838996 27.073421193470697 27.462258050129336 22.841907501560968 58-59 22.91627457049302 26.681334408457325 27.48354088043022 22.91885014061944 60-61 22.637762440487474 26.76270808478375 27.97797897131134 22.62155050341744 62-63 22.519974458204075 26.734315037113543 27.88163315411633 22.864077350566056 64-65 22.362211361598234 26.81062586919463 27.710780639218818 23.11638212998831 66-67 22.383659020505505 26.978893276877468 27.38007868050812 23.257369022108907 68-69 22.28375220163835 26.745809876786296 27.725273442452014 23.245164479123336 70-71 22.372684026346136 26.78160524817631 27.54435156305574 23.301359162421818 72-73 22.499323775431453 26.63543211586152 27.546634966061667 23.318609142645364 74-75 22.24952282176876 27.109008480554575 27.235501547519192 23.405967150157473 76-77 22.394552507564818 26.845117055912194 27.349705813676007 23.410624622846985 78-79 22.355646517361276 26.92169841025263 27.36119578300473 23.36145928938136 80-81 22.314392187955225 27.11502531493284 27.270196281181786 23.30038621593015 82-83 22.384277282067195 26.991800826662914 27.22412764829664 23.39979424297325 84-85 22.223114191974634 26.9290351094904 27.124666375438544 23.72318432309642 86-87 22.217484808154822 26.830086617291403 27.26117517533517 23.69125339921861 88-89 22.2534027687639 26.837528701420187 27.0200764476034 23.888992082212503 90-91 22.241925660477172 26.910356980108418 27.120675823205907 23.7270415362085 92-93 22.294188565695077 26.811622400322538 27.148521667151865 23.74566736683052 94-95 22.110792565454332 26.662728608354634 27.16283525480921 24.063643571381824 96-97 22.047781120935927 26.497815004697344 27.27747600652834 24.17692786783839 98-99 21.93101639730295 26.643838930778017 26.94134429207371 24.48380037984532 100-101 22.031655885243346 26.10183366332836 27.534452127603824 24.332058323824466 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1480.0 1 998.5 2 706.5 3 983.5 4 889.0 5 803.5 6 1410.5 7 2911.0 8 4499.5 9 5174.0 10 4631.5 11 3952.0 12 4097.0 13 4366.0 14 4673.0 15 5070.0 16 5403.0 17 5821.0 18 6214.0 19 6750.0 20 7581.5 21 8826.5 22 10597.5 23 12765.5 24 14959.0 25 17889.5 26 22400.0 27 27716.5 28 32652.5 29 38168.5 30 45560.5 31 54338.5 32 64232.5 33 72424.5 34 80252.0 35 88112.5 36 97614.0 37 107310.5 38 113476.5 39 117402.0 40 120095.5 41 127006.0 42 134284.5 43 140616.0 44 148770.0 45 156515.5 46 160090.5 47 167777.0 48 189727.5 49 191694.0 50 175310.5 51 167357.5 52 161412.5 53 160666.0 54 159437.5 55 150083.5 56 136707.5 57 123286.0 58 112207.0 59 99157.5 60 83915.0 61 69275.0 62 57001.0 63 45490.0 64 34659.0 65 27202.0 66 20541.5 67 16280.0 68 11899.0 69 7502.0 70 5454.0 71 4252.5 72 3414.5 73 2895.5 74 2393.0 75 1966.5 76 1403.5 77 815.0 78 503.0 79 334.5 80 242.0 81 166.5 82 108.5 83 64.5 84 37.0 85 27.0 86 14.5 87 7.5 88 7.0 89 5.0 90 2.5 91 2.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23757614355539589 2 0.0 3 0.0 4 0.0 5 0.030148577631583934 6 0.0043037540553962274 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0029769490486808103 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.125360104913482E-4 26-27 0.0011595606781378436 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006957364068827062 36-37 0.016992023783481476 38-39 0.0130227583852404 40-41 0.008685555464128657 42-43 0.007258403860266694 44-45 0.003344886571551472 46-47 5.909299609740934E-4 48-49 0.0014606004695774761 50-51 4.45984876206863E-5 52-53 0.0 54-55 0.0 56-57 0.0012710568971895594 58-59 2.898901695344609E-4 60-61 0.002386019087706717 62-63 9.477178619395838E-4 64-65 0.0013714034943361037 66-67 0.005485613977344415 68-69 2.7874054762928933E-4 70-71 8.91969752413726E-5 72-73 0.0 74-75 4.7943374192237773E-4 76-77 0.059951516984107556 78-79 0.14242527021666168 80-81 0.0010815133248016427 82-83 9.254186181292407E-4 84-85 0.0 86-87 0.10594370734294029 88-89 0.35761297298647304 90-91 0.39397189001923755 92-93 6.80126936215466E-4 94-95 3.010397914396325E-4 96-97 1.783939504827452E-4 98-99 0.0 100-101 0.0010257652152757847 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4484457.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.717647822909576 #Duplication Level Percentage of deduplicated Percentage of total 1 67.11339106427087 27.99812812620459 2 15.817762494822524 13.197596902108671 3 6.437445868364023 8.056652988463638 4 3.3264921240344725 5.550937076646103 5 1.9344449285088126 4.035024613017208 6 1.2290487942253603 3.0763814852799145 7 0.8054993480592918 2.3522476686744556 8 0.5681599125983914 1.8961836112699821 9 0.40851878664181585 1.533819858314905 >10 2.092511435965979 15.501987679256818 >50 0.15856805263810292 4.589341385217634 >100 0.09921879627624128 7.66267924273079 >500 0.0060481347865498514 1.7364782028622818 >1k 0.002676165562644199 1.7940636952700473 >5k 1.0704662250575611E-4 0.39197974026065946 >10k+ 1.0704662250575611E-4 0.6264977244223061 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15197 0.3388816081857848 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12861 0.2867905746448232 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8975 0.20013571319782977 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8580 0.1913275118927442 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.229924381034315E-5 0.0 12-13 0.0 0.0 0.0 6.689773143102944E-5 0.0 14-15 0.0 0.0 0.0 1.3379546286205888E-4 0.0 16-17 0.0 0.0 0.0 1.672443285775736E-4 0.0 18-19 1.1149621905171574E-5 0.0 0.0 2.0069319429308832E-4 0.0 20-21 2.229924381034315E-5 0.0 0.0 2.898901695344609E-4 0.0 22-23 2.229924381034315E-5 0.0 0.0 5.128826076378924E-4 0.0 24-25 2.229924381034315E-5 0.0 0.0 7.916231552671817E-4 0.0 26-27 2.229924381034315E-5 0.0 0.0 0.0012822065190947309 0.0 28-29 4.45984876206863E-5 0.0 0.0 0.003946966154430737 0.0 30-31 4.45984876206863E-5 0.0 0.0 0.013725184565266207 0.0 32-33 4.45984876206863E-5 0.0 0.0 0.0318879186487907 0.0 34-35 4.45984876206863E-5 0.0 0.0 0.05972852454600412 1.1149621905171574E-5 36-37 4.45984876206863E-5 0.0 0.0 0.10887605790400041 2.229924381034315E-5 38-39 4.45984876206863E-5 0.0 0.0 0.17950891267326233 2.229924381034315E-5 40-41 4.45984876206863E-5 0.0 0.0 0.25668659550086 2.229924381034315E-5 42-43 4.45984876206863E-5 0.0 0.0 0.3367074319142763 2.229924381034315E-5 44-45 4.45984876206863E-5 0.0 0.0 0.4356268774569586 2.229924381034315E-5 46-47 5.574810952585787E-5 0.0 0.0 0.5585514589614752 2.229924381034315E-5 48-49 6.689773143102944E-5 0.0 0.0 0.6896375637005774 2.229924381034315E-5 50-51 6.689773143102944E-5 0.0 0.0 0.8290412863809375 2.229924381034315E-5 52-53 6.689773143102944E-5 0.0 0.0 0.9712435641594959 2.229924381034315E-5 54-55 6.689773143102944E-5 0.0 0.0 1.1266581438956824 2.229924381034315E-5 56-57 6.689773143102944E-5 0.0 0.0 1.3130798221501512 2.229924381034315E-5 58-59 6.689773143102944E-5 0.0 0.0 1.5234062897693077 2.229924381034315E-5 60-61 6.689773143102944E-5 0.0 0.0 1.7557978591387986 2.229924381034315E-5 62-63 6.689773143102944E-5 0.0 0.0 1.9845435021452988 3.344886571551472E-5 64-65 6.689773143102944E-5 0.0 0.0 2.220302257330152 4.45984876206863E-5 66-67 6.689773143102944E-5 0.0 0.0 2.4936352383354325 4.45984876206863E-5 68-69 6.689773143102944E-5 0.0 0.0 2.80503302852497 4.45984876206863E-5 70-71 6.689773143102944E-5 0.0 0.0 3.1490434627871338 4.45984876206863E-5 72-73 6.689773143102944E-5 0.0 0.0 3.4900211998910904 4.45984876206863E-5 74-75 6.689773143102944E-5 0.0 0.0 3.840910950868745 4.45984876206863E-5 76-77 6.689773143102944E-5 0.0 0.0 4.217444832228294 4.45984876206863E-5 78-79 6.689773143102944E-5 0.0 0.0 4.642278875681047 4.45984876206863E-5 80-81 6.689773143102944E-5 0.0 0.0 5.106013949960943 4.45984876206863E-5 82-83 6.689773143102944E-5 0.0 0.0 5.57436496770958 4.45984876206863E-5 84-85 6.689773143102944E-5 0.0 0.0 6.04778906342507 4.45984876206863E-5 86-87 6.689773143102944E-5 0.0 0.0 6.538104836326895 4.45984876206863E-5 88-89 6.689773143102944E-5 0.0 0.0 7.0763193849333375 4.45984876206863E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 21025 0.0 27.107792 1 TACATGG 21110 0.0 26.43899 2 ACATGGG 20905 0.0 25.472319 3 GTATCAA 28430 0.0 23.360487 1 GGTATCA 21525 0.0 23.207024 1 GAGTACT 15305 0.0 22.040508 12-13 CATGGGA 11505 0.0 21.615757 4 GTACTTT 16740 0.0 20.037766 14-15 CATGGGG 9290 0.0 19.923914 4 TCAACGC 33250 0.0 19.783237 4 ATCAACG 33255 0.0 19.765991 3 CAACGCA 33595 0.0 19.594202 5 TATCAAC 33915 0.0 19.423317 2 AGTACTT 16145 0.0 19.350985 12-13 AACGCAG 34130 0.0 19.281681 6 GTATAGG 1150 0.0 19.02976 1 TACCTGG 3350 0.0 18.275558 2 AGAGTAC 28285 0.0 17.931097 10-11 CGTATAG 215 2.3649107E-5 17.702103 1 ATGGGAG 5440 0.0 17.535694 5 >>END_MODULE