FastQCFastQC Report
Thu 26 May 2016
SRR938703_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938703_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984961
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114410.38328808986114055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91240.30566563516240247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60030.201108155181927No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109550.030.3746071
GTATCAA157300.028.6875951
GTACATG137650.024.8626231
TACATGG140200.023.957222
TATCAAC190550.023.5439472
ATCAACG189350.023.5430413
TCAACGC189350.023.5430414
ACATGGG139600.023.2118033
CAACGCA191850.023.1872545
AACGCAG196100.022.8860286
TACCTGG22950.021.8807092
GAGTACT101450.021.84123412-13
CTATACG1553.9901264E-521.3946194
ACGCAGA217900.020.3594347
CGCAGAG219850.020.0705038
GTACTTT110200.019.91384514-15
CATGGGA75450.019.5271744
AGTACTT104700.019.24138312-13
CATGGGG65100.019.1387254
GCAGAGT229700.018.7364049
Produced by FastQC (version 0.11.5)