##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938703_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2984961 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.450262499242033 33.0 31.0 34.0 30.0 34.0 2 31.506277636458233 33.0 31.0 34.0 28.0 34.0 3 31.51931264763593 33.0 31.0 34.0 28.0 34.0 4 35.068484646868086 37.0 35.0 37.0 32.0 37.0 5 35.0963054458668 37.0 35.0 37.0 32.0 37.0 6 35.182074740675006 37.0 35.0 37.0 32.0 37.0 7 35.08052031500579 37.0 35.0 37.0 32.0 37.0 8 35.047931614516905 37.0 35.0 37.0 32.0 37.0 9 36.590356791931285 39.0 37.0 39.0 32.0 39.0 10-11 36.478554493676796 39.0 37.0 39.0 32.0 39.0 12-13 36.343724256363814 39.0 37.0 39.0 32.0 39.0 14-15 37.481306455930245 40.0 37.0 41.0 32.0 41.0 16-17 37.404164409518245 40.0 37.0 41.0 32.0 41.0 18-19 37.33008957235957 40.0 37.0 41.0 31.5 41.0 20-21 37.22113471499293 40.0 37.0 41.0 31.0 41.0 22-23 37.13041125160429 40.0 37.0 41.0 31.0 41.0 24-25 37.13690681385787 40.0 37.0 41.0 31.0 41.0 26-27 37.04076150408665 40.0 37.0 41.0 30.5 41.0 28-29 36.906906153882744 40.0 36.0 41.0 30.0 41.0 30-31 36.695078763173115 39.0 36.0 41.0 30.0 41.0 32-33 36.48234516296863 39.0 35.5 41.0 30.0 41.0 34-35 36.15040079250616 39.0 35.0 41.0 29.5 41.0 36-37 35.893166108367915 39.0 35.0 41.0 27.0 41.0 38-39 35.721867387882114 39.0 35.0 41.0 27.0 41.0 40-41 35.52925230848912 39.0 35.0 40.0 26.5 41.0 42-43 35.26323560006311 38.0 35.0 40.0 25.5 41.0 44-45 35.22479355676674 39.0 35.0 40.5 25.0 41.0 46-47 35.02942567758842 39.0 35.0 41.0 24.0 41.0 48-49 34.796488798346104 39.0 34.0 41.0 24.0 41.0 50-51 33.64453187160569 38.0 32.5 39.5 22.0 40.5 52-53 33.7317165952922 37.5 33.0 39.5 22.0 40.5 54-55 34.240874001368866 38.0 34.0 40.0 22.5 41.0 56-57 34.14329919218375 38.0 34.0 40.0 22.0 41.0 58-59 33.91357558775475 38.0 33.5 40.0 21.5 41.0 60-61 33.61162373645753 37.5 33.0 40.0 20.0 41.0 62-63 33.23848368538148 37.0 33.0 40.0 18.5 41.0 64-65 32.84037094622007 36.0 32.0 39.5 17.5 41.0 66-67 32.448792965804245 36.0 32.0 39.0 13.5 41.0 68-69 32.04457545676476 35.0 31.0 39.0 12.0 40.5 70-71 31.641576221598875 35.0 31.0 38.5 8.0 40.0 72-73 31.23244374047098 35.0 31.0 37.5 7.0 40.0 74-75 30.783040381432116 35.0 30.5 37.0 4.5 39.0 76-77 30.353254196620995 35.0 30.0 36.0 2.0 39.0 78-79 29.94394935143206 34.0 30.0 36.0 2.0 38.5 80-81 29.523757596832922 34.0 29.0 35.5 2.0 37.0 82-83 29.086614699488536 34.0 29.0 35.0 2.0 37.0 84-85 28.69577441715319 34.0 29.0 35.0 2.0 36.0 86-87 28.320993473616575 34.0 29.0 35.0 2.0 36.0 88-89 27.963107055670076 34.0 27.5 35.0 2.0 36.0 90-91 27.567788155356133 34.0 26.5 35.0 2.0 35.0 92-93 27.13515905232933 33.5 26.0 35.0 2.0 35.0 94-95 26.64885152603334 33.0 25.0 35.0 2.0 35.0 96-97 26.070675797774243 33.0 24.0 35.0 2.0 35.0 98-99 25.37281642875736 33.0 20.0 35.0 2.0 35.0 100-101 23.169021471302305 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10669549028387593 1101 2 -0.03836997289506883 1101 3 0.21276785847557278 1101 4 0.1436289739764831 1101 5 0.07968286236690147 1101 6 -0.05173061564222081 1101 7 0.14824373428591997 1101 8 0.20436653625377232 1101 9 0.2463675692714986 1101 10-11 0.1809114803082963 1101 12-13 0.21182031232026333 1101 14-15 0.3710133848285224 1101 16-17 0.3699465908270696 1101 18-19 0.41301334485047647 1101 20-21 0.3958655789151351 1101 22-23 0.3460497337636568 1101 24-25 0.3049032385156636 1101 26-27 0.39522727672200375 1101 28-29 0.31254095600930043 1101 30-31 0.28712520645314044 1101 32-33 0.24775106452621287 1101 34-35 0.33659038549635767 1101 36-37 0.3763549410859284 1101 38-39 0.32500629765687705 1101 40-41 0.24088545379240145 1101 42-43 0.2507839166013497 1101 44-45 0.5889038450474757 1101 46-47 0.5838995839543983 1101 48-49 0.39241952670842295 1101 50-51 0.3699451888898935 1101 52-53 0.3954564540760046 1101 54-55 0.41589249002928597 1101 56-57 0.25991670650818577 1101 58-59 0.2516495051125389 1101 60-61 0.29325073876809427 1101 62-63 0.17203334497574474 1101 64-65 0.28080365257726214 1101 66-67 0.24774986870817628 1101 68-69 0.12168932528594922 1101 70-71 0.015252063223844203 1101 72-73 0.11898281827704338 1101 74-75 -0.048930980555859094 1101 76-77 0.0012812007871119135 1101 78-79 -0.16615396760043666 1101 80-81 -0.1304885190633307 1101 82-83 -0.21716697701308263 1101 84-85 -0.14422938538525543 1101 86-87 -0.295284309700218 1101 88-89 -0.4163896962849343 1101 90-91 -0.5622147735738316 1101 92-93 -0.5818720871473033 1101 94-95 -0.6888242683081778 1101 96-97 -0.8947203941085284 1101 98-99 -0.9749076460346551 1101 100-101 -0.6437884296350731 1102 1 0.2215743423548453 1102 2 0.20901662109048758 1102 3 0.28867716251073006 1102 4 0.2884397217897501 1102 5 0.21343869761999912 1102 6 0.28663083417477964 1102 7 0.2730690284100987 1102 8 0.11210421841072815 1102 9 0.29784417825025145 1102 10-11 0.28757043717796904 1102 12-13 0.39956779999597813 1102 14-15 0.2742096600082178 1102 16-17 0.3615401968816627 1102 18-19 0.42512323144741515 1102 20-21 0.407842404126022 1102 22-23 0.33030563392185996 1102 24-25 0.2971599052398659 1102 26-27 0.30365308308819294 1102 28-29 0.3533944664772264 1102 30-31 0.4520311791655658 1102 32-33 0.3354113854671965 1102 34-35 0.38428783830774904 1102 36-37 0.4573892069642156 1102 38-39 0.30503609489520755 1102 40-41 0.2705277001381461 1102 42-43 0.44535205644454834 1102 44-45 0.5385659128698848 1102 46-47 0.4729716087644604 1102 48-49 0.42292882965592327 1102 50-51 0.2899022332932546 1102 52-53 0.31116989837146036 1102 54-55 0.24548034140060793 1102 56-57 0.264815150700791 1102 58-59 0.1346541435225106 1102 60-61 0.1197170563264578 1102 62-63 0.10399198256902054 1102 64-65 0.17251911235961614 1102 66-67 0.1890905458162493 1102 68-69 0.10474530848486552 1102 70-71 0.17192690943183564 1102 72-73 0.08717186640873464 1102 74-75 0.07747615394620766 1102 76-77 -0.032837015417126025 1102 78-79 -0.1757202595987124 1102 80-81 -0.1385770080179931 1102 82-83 -0.13771679777330448 1102 84-85 -0.1900640379041043 1102 86-87 -0.3070765868670229 1102 88-89 -0.4081372894166755 1102 90-91 -0.36239044297722955 1102 92-93 -0.4691728763414176 1102 94-95 -0.6155420251875903 1102 96-97 -0.461309312571867 1102 98-99 -0.6920258189688191 1102 100-101 -0.7047463864931558 1103 1 0.10929168428825164 1103 2 0.15916228441030356 1103 3 0.1771471663756259 1103 4 0.29310912151675694 1103 5 0.2032755430253843 1103 6 0.16424643947447493 1103 7 0.11815693432619412 1103 8 0.0991166549639928 1103 9 0.227851873009989 1103 10-11 0.1455805185976331 1103 12-13 0.17803350693889541 1103 14-15 0.30371544392208705 1103 16-17 0.35567425617685444 1103 18-19 0.4742793766880524 1103 20-21 0.39093354908059297 1103 22-23 0.42747124173198614 1103 24-25 0.2027635352048307 1103 26-27 0.2680875883057965 1103 28-29 0.3436075844662696 1103 30-31 0.34804490829834833 1103 32-33 0.36053506792933376 1103 34-35 0.5123578314303359 1103 36-37 0.45373571402202373 1103 38-39 0.29692705582941414 1103 40-41 0.42890301992606084 1103 42-43 0.48586303986753876 1103 44-45 0.5781840589582288 1103 46-47 0.4438517304794516 1103 48-49 0.43480059455824716 1103 50-51 0.4931246328673069 1103 52-53 0.32232321324826785 1103 54-55 0.4318028767713997 1103 56-57 0.43215323088742963 1103 58-59 0.5143737810397724 1103 60-61 0.4974469737625 1103 62-63 0.4735387156822881 1103 64-65 0.25933643596104616 1103 66-67 0.2154547309715582 1103 68-69 0.2457429650276879 1103 70-71 0.21085159303008894 1103 72-73 0.26865528531075356 1103 74-75 0.27404387075102576 1103 76-77 0.2780633882366601 1103 78-79 0.2998674437315181 1103 80-81 0.08998999185473266 1103 82-83 0.23872328410174148 1103 84-85 0.35256487267438175 1103 86-87 0.303885701804397 1103 88-89 0.2714658758268307 1103 90-91 0.22057453827627782 1103 92-93 0.20910951083226692 1103 94-95 0.14207619562982998 1103 96-97 0.12849688223516154 1103 98-99 0.09154201576782484 1103 100-101 0.2917559423684999 1104 1 0.08103291475589813 1104 2 0.1193358372725335 1104 3 -0.03675072818264624 1104 4 0.11870569475483705 1104 5 0.026397586076676305 1104 6 0.0722478720042119 1104 7 0.08849862094146488 1104 8 0.004165144210595884 1104 9 0.08551861664371785 1104 10-11 0.16221335510075363 1104 12-13 0.22015153042484314 1104 14-15 0.30764317892293036 1104 16-17 0.2575913249571329 1104 18-19 0.15936451560573772 1104 20-21 0.2188639323765429 1104 22-23 0.2785211352347048 1104 24-25 0.2792161509852704 1104 26-27 0.26569905702575625 1104 28-29 0.27196607738844136 1104 30-31 0.250091387762275 1104 32-33 0.3416990690225816 1104 34-35 0.16470059479903654 1104 36-37 0.26405281437450157 1104 38-39 0.32118988026912376 1104 40-41 0.2801993061869652 1104 42-43 0.353943603172155 1104 44-45 0.38313948089478345 1104 46-47 0.407470599817664 1104 48-49 0.32090364245374303 1104 50-51 0.4241103923907943 1104 52-53 0.36054295341376275 1104 54-55 0.4837057498417252 1104 56-57 0.41613134004241914 1104 58-59 0.3510443628164879 1104 60-61 0.24227066422297838 1104 62-63 0.40356865037023937 1104 64-65 0.23062776255897433 1104 66-67 0.2165129298481503 1104 68-69 0.2623795713459032 1104 70-71 0.22927733002893902 1104 72-73 0.22679095859972875 1104 74-75 0.1230352685042142 1104 76-77 0.10121311586751958 1104 78-79 0.25258677628575654 1104 80-81 0.14507076014304943 1104 82-83 0.1693692301368408 1104 84-85 0.0957748466626569 1104 86-87 0.07962658827641533 1104 88-89 0.05076028424860013 1104 90-91 0.1505009797593253 1104 92-93 0.11850065035312696 1104 94-95 0.021035031605176613 1104 96-97 0.11519438284775774 1104 98-99 0.20452826301114513 1104 100-101 0.4787284627726258 1105 1 0.2292924013919837 1105 2 0.10945508249124103 1105 3 0.2566486404864463 1105 4 0.26872181147695784 1105 5 0.25525341921858313 1105 6 0.19401619134501402 1105 7 0.197794542484516 1105 8 0.22065394030997965 1105 9 0.20393600084413777 1105 10-11 0.30323664191171673 1105 12-13 0.10233875486594712 1105 14-15 0.36138955266045514 1105 16-17 0.3300496530417263 1105 18-19 0.4061894518798468 1105 20-21 0.43851760346944246 1105 22-23 0.5323687710783034 1105 24-25 0.49837705793064657 1105 26-27 0.45579556284630485 1105 28-29 0.40734364865399186 1105 30-31 0.44568182017207647 1105 32-33 0.4780189822516334 1105 34-35 0.37241307155020564 1105 36-37 0.4159954119825571 1105 38-39 0.5632430163609499 1105 40-41 0.41050404654101413 1105 42-43 0.4740760520617755 1105 44-45 0.6117264704392937 1105 46-47 0.6254132585581047 1105 48-49 0.524266109386673 1105 50-51 0.582801288480546 1105 52-53 0.5286515958670677 1105 54-55 0.4835898530109759 1105 56-57 0.3750553496976323 1105 58-59 0.5688105712371936 1105 60-61 0.5518124584031838 1105 62-63 0.4741449574919798 1105 64-65 0.42089886620247086 1105 66-67 0.3001192363029972 1105 68-69 0.3183732552580807 1105 70-71 0.3267829199380152 1105 72-73 0.45744545034870754 1105 74-75 0.34519351526419584 1105 76-77 0.38082666651107644 1105 78-79 0.3103469316731875 1105 80-81 0.3127754425139493 1105 82-83 0.1939839339596503 1105 84-85 0.13278518544727191 1105 86-87 0.011648060566766816 1105 88-89 0.15508340970709256 1105 90-91 0.12715627827065745 1105 92-93 0.21821302706946355 1105 94-95 0.19333101150187915 1105 96-97 0.3513650311475338 1105 98-99 0.33555038274912974 1105 100-101 0.30342564901524227 1106 1 -0.03350934721133214 1106 2 0.08635287548670689 1106 3 0.0406989367499655 1106 4 -0.03582820926747132 1106 5 -0.04784242186899945 1106 6 -0.103909872025298 1106 7 -0.014721061831423299 1106 8 0.05244539397447312 1106 9 -0.034532674152799814 1106 10-11 0.10551776981972694 1106 12-13 0.027338800862459323 1106 14-15 0.09948186121607705 1106 16-17 0.03456209463764992 1106 18-19 0.17414809917769247 1106 20-21 0.14623531184972904 1106 22-23 0.03526183655248616 1106 24-25 -0.05692385583601123 1106 26-27 -0.18874030477206105 1106 28-29 0.07451267386835525 1106 30-31 -0.0022175882208088638 1106 32-33 0.09279487805665809 1106 34-35 0.11142830007466387 1106 36-37 0.3469897231342287 1106 38-39 0.075280072989095 1106 40-41 0.22499540793651107 1106 42-43 0.2541713384314477 1106 44-45 0.12172507674504374 1106 46-47 0.20533552905622088 1106 48-49 0.38203282704813546 1106 50-51 0.32531892124257666 1106 52-53 0.34613744504083144 1106 54-55 0.2891926188731091 1106 56-57 0.4766788885035069 1106 58-59 0.5053878237552709 1106 60-61 0.3939622110799448 1106 62-63 0.25773586361402323 1106 64-65 0.24406746916887556 1106 66-67 0.157373207871629 1106 68-69 0.40523835393439356 1106 70-71 0.2824582682925829 1106 72-73 0.19173982709139992 1106 74-75 0.15620961036290382 1106 76-77 0.0613507783414704 1106 78-79 0.14480629789647637 1106 80-81 0.1767091422248157 1106 82-83 0.28906469923118294 1106 84-85 0.21007208591726467 1106 86-87 0.3043658241428666 1106 88-89 0.5149640119669918 1106 90-91 0.4010453705663366 1106 92-93 0.4524588774943332 1106 94-95 0.4257303320414394 1106 96-97 0.5212921220798741 1106 98-99 0.6135098211869412 1106 100-101 0.791484495109998 1107 1 0.08184074008456932 1107 2 0.11685139333718197 1107 3 0.033610904132583386 1107 4 0.15176233213225743 1107 5 0.07942977161138032 1107 6 0.09140289317671346 1107 7 -0.18294870275030917 1107 8 0.021155341303348507 1107 9 0.03428452373594837 1107 10-11 0.02199226776508567 1107 12-13 0.1738625386499777 1107 14-15 0.18369568433614347 1107 16-17 0.16355726973864648 1107 18-19 0.2106492996683471 1107 20-21 0.21658213759096157 1107 22-23 0.23024190941681155 1107 24-25 0.10282282549771082 1107 26-27 0.22587382257627553 1107 28-29 0.1528526021783847 1107 30-31 0.21569772787475472 1107 32-33 0.18346437752040856 1107 34-35 0.15619267912695278 1107 36-37 0.22895871588724503 1107 38-39 0.276242529788405 1107 40-41 0.3221430614061873 1107 42-43 0.3615427730842953 1107 44-45 0.31498285608045506 1107 46-47 0.29417332841051547 1107 48-49 0.3208583783701471 1107 50-51 0.5408940374612641 1107 52-53 0.45469744342610596 1107 54-55 0.3688877437892728 1107 56-57 0.25368304732975133 1107 58-59 0.16173991936148724 1107 60-61 0.11937382753580295 1107 62-63 0.186486146878444 1107 64-65 0.17481275963990583 1107 66-67 0.2538085717482801 1107 68-69 0.29833183990787404 1107 70-71 0.28474881088500226 1107 72-73 0.17726616219573188 1107 74-75 0.16974953481960853 1107 76-77 0.07427405795166209 1107 78-79 0.11624234986483728 1107 80-81 0.057786826860461105 1107 82-83 0.09380973373209756 1107 84-85 0.07735536684858957 1107 86-87 0.11155324013833834 1107 88-89 0.22074700986326334 1107 90-91 0.31300873931651196 1107 92-93 0.3873012953201318 1107 94-95 0.3099578711227515 1107 96-97 0.22684655884929228 1107 98-99 0.2780538778636803 1107 100-101 0.2667755808361356 1108 1 0.06281976533027134 1108 2 0.009050317255525187 1108 3 -0.01895406520586107 1108 4 -0.06561773698353335 1108 5 0.050917824843907056 1108 6 -0.04403844341486263 1108 7 0.021569844247842695 1108 8 0.02778024658653777 1108 9 0.05778057333279207 1108 10-11 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2316 98-99 -3.290383495551925 2316 100-101 -3.3326407053399656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12751.0 3 4194.0 4 4014.0 5 4581.0 6 5143.0 7 6402.0 8 7447.0 9 9197.0 10 10912.0 11 13472.0 12 16133.0 13 19794.0 14 21077.0 15 20152.0 16 23922.0 17 28768.0 18 25334.0 19 20278.0 20 20894.0 21 22330.0 22 24402.0 23 27291.0 24 30609.0 25 34917.0 26 40569.0 27 46544.0 28 54450.0 29 64904.0 30 76928.0 31 91826.0 32 112206.0 33 142695.0 34 188183.0 35 261900.0 36 382582.0 37 553321.0 38 478625.0 39 76171.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54095383566388 18.381040328360854 13.91483475921904 23.163171076756228 2 15.608397123699437 19.826743264287273 40.2593888196625 24.30547079235079 3 19.627422952873275 24.353239360869217 29.23195466145469 26.787383024802814 4 12.98499344041509 15.980134786726296 36.3248416443766 34.71003012848201 5 14.058864806699958 37.03731159048889 33.691290160709926 15.212533442101222 6 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28.8098534750083 22.322692957710586 28-29 22.3327521585355 26.33432587327506 28.996783674402643 22.336138293786796 30-31 22.397781370978244 26.09052954414756 28.980942163086194 22.530746921787998 32-33 22.29415641995397 26.211065662031018 28.86749893956181 22.6272789784532 34-35 22.972914396428237 26.17221587703532 28.500955474260998 22.353914252275448 36-37 22.857418402970048 26.056618464099866 28.45894287829208 22.627020254638005 38-39 22.599568048367814 26.27824113064644 28.717430098127227 22.404760722858523 40-41 22.755450013567522 26.07721324291011 28.768259166740545 22.399077576781824 42-43 22.595656696216945 26.256826430910625 28.366534977459928 22.78098189541251 44-45 22.601435089755377 26.379030509962426 28.284388501509948 22.73514589877225 46-47 22.7292486649569 26.530412694972 27.85346476733284 22.88687387273826 48-49 22.95360307452305 26.567450278187643 27.704708705032893 22.774237942256413 50-51 22.74418886463433 26.760288877930392 27.68391058989062 22.811611667544657 52-53 22.997254098402045 26.7141687108603 27.575574570736165 22.71300262000149 54-55 22.616038839807672 26.5154359691666 27.78186655455583 23.086658636469895 56-57 22.743297610814686 26.988808738976584 27.508526795254273 22.75936685495446 58-59 22.906567816747458 26.53444060046784 27.580052705377277 22.978938877407426 60-61 22.894811590386112 26.402672732987536 28.052092461950533 22.650423214675815 62-63 22.776204442694425 26.49496280366629 27.97441942252713 22.754413331112154 64-65 22.56502273253716 26.436119281177785 27.904256694516715 23.094601291768342 66-67 22.521045353861258 26.76617511741057 27.586710763457393 23.12606876527078 68-69 22.432029141155162 26.647351245639257 27.849427263364905 23.071192349840672 70-71 22.486193314473233 26.57373655611666 27.696571443578954 23.243498685831153 72-73 22.627600589923524 26.486445175442658 27.683799374153157 23.20215486048066 74-75 22.483007230038364 26.849561237177678 27.47439827810862 23.193033254675335 76-77 22.63494494964004 26.712109867462097 27.463701565974517 23.189243616923346 78-79 22.622657788438115 26.778892940354616 27.504085115776356 23.094364155430917 80-81 22.482508063461786 26.987530385735415 27.43478350852332 23.09517804227948 82-83 22.61207271415282 26.86249914175975 27.338603713790675 23.186824430296756 84-85 22.60885997944987 26.6759242088655 27.23926898623518 23.475946825449444 86-87 22.669755488816204 26.59474187774693 27.534465246964125 23.20103738647274 88-89 22.69974533721359 26.58612163556459 27.32814469828435 23.38598832893747 90-91 22.668928005989542 26.737386261151702 27.409577005437786 23.18410872742097 92-93 22.762151221939362 26.57011986768299 27.38674917739515 23.280979732982498 94-95 22.679378430502172 26.44658259826931 27.37082660636339 23.503212364865124 96-97 22.77417642158273 26.30626432852808 27.34563654393925 23.573922705949947 98-99 22.730211503602376 26.310083305012892 27.158450877883112 23.80125431350162 100-101 22.933796008234033 25.957300635964803 27.53230945922543 23.576593896575734 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2851.0 1 1734.0 2 855.0 3 1116.0 4 958.5 5 900.0 6 1495.0 7 2911.0 8 4575.5 9 5245.5 10 4573.0 11 3804.0 12 3747.0 13 4013.0 14 4340.0 15 4638.5 16 4962.5 17 5174.0 18 5295.5 19 5569.5 20 5915.0 21 6580.5 22 7464.0 23 8632.0 24 10057.5 25 11979.0 26 15000.0 27 18492.5 28 21813.5 29 25613.5 30 30283.0 31 35104.5 32 40458.5 33 45321.0 34 50387.0 35 55816.5 36 61719.0 37 67817.0 38 71946.5 39 74556.5 40 76954.0 41 82362.0 42 88133.5 43 92814.0 44 97885.0 45 102080.0 46 104694.0 47 108864.5 48 120238.5 49 122883.5 50 116578.5 51 113438.5 52 109181.0 53 108441.0 54 107930.5 55 101525.0 56 92413.0 57 83532.0 58 76122.0 59 67971.5 60 57939.5 61 47396.5 62 38887.5 63 31321.5 64 24233.0 65 19021.5 66 14446.5 67 11765.0 68 9037.0 69 5984.0 70 4313.0 71 3277.0 72 2651.0 73 2135.0 74 1647.0 75 1375.5 76 1059.0 77 640.0 78 407.5 79 287.0 80 194.5 81 149.0 82 110.5 83 82.0 84 71.0 85 51.0 86 35.0 87 27.5 88 14.5 89 11.5 90 9.5 91 11.0 92 10.0 93 6.5 94 6.0 95 2.5 96 2.5 97 3.0 98 4.0 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16164365296565014 2 0.09370306680723801 3 0.08703631303725577 4 0.10268140856781714 5 0.123586204308867 6 0.03845946395949562 7 0.04274762718842893 8 0.04613125598625911 9 0.046834782766005986 10-11 0.045226721555155995 12-13 0.04968239116021952 14-15 0.047454556357687756 16-17 0.046064253435807036 18-19 0.050201660926223156 20-21 0.05184322341229919 22-23 0.06539448924123296 24-25 0.08212837621664068 26-27 0.08376993870271672 28-29 0.07402106761193865 30-31 0.062228618732372044 32-33 0.05107269408210024 34-35 0.2999034158235233 36-37 0.47685715156747444 38-39 0.45439454652841355 40-41 0.42846455950345746 42-43 0.45888371740870315 44-45 0.46891734933890256 46-47 0.46866608977470725 48-49 0.48523247037398476 50-51 0.47424405209984316 52-53 0.4808103020441473 54-55 0.47208321984776347 56-57 0.45045814668935374 58-59 0.428096045475971 60-61 0.3961358289103275 62-63 0.45447829971647874 64-65 0.49478033381340664 66-67 0.496756909051743 68-69 0.507226057559881 70-71 0.5032896577208211 72-73 0.4939930538455946 74-75 0.48598624906657073 76-77 0.4690011025269677 78-79 0.4421163291580694 80-81 0.44816330933636994 82-83 0.46114505348646095 84-85 0.45863245784450785 86-87 0.47014014588465314 88-89 0.48127931989731193 90-91 0.4840934270162993 92-93 0.48640501500689626 94-95 0.4905089212220863 96-97 0.49268650411177906 98-99 0.4854837299381801 100-101 0.4730547568293187 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2984961.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.14432730189624 #Duplication Level Percentage of deduplicated Percentage of total 1 76.21970617551315 40.50645011845833 2 13.156280299163814 13.983633325885023 3 4.600195182622315 7.334228353136599 4 1.994146873894353 4.239103766171789 5 1.0723198152199875 2.849385761617996 6 0.6600978168053285 2.1048272656541718 7 0.4223774186068705 1.5712874645561494 8 0.2899731864406633 1.2328343943181113 9 0.2099579800318258 1.0042268049410712 >10 1.2018238851835858 11.820999997558953 >50 0.10401569904595173 3.816534959054121 >100 0.06385754082119773 6.28041983408145 >500 0.003920287377936076 1.4658135984860952 >1k 0.001138147948307179 0.8974794155377299 >5k 1.264608831452421E-4 0.5083185232454411 >10k+ 6.323044157262105E-5 0.3844564172969585 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11441 0.38328808986114055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9124 0.30566563516240247 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6003 0.201108155181927 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.350127522604148E-5 0.0 10-11 0.0 0.0 0.0 3.350127522604148E-5 0.0 12-13 0.0 0.0 0.0 3.350127522604148E-5 0.0 14-15 0.0 0.0 0.0 3.350127522604148E-5 0.0 16-17 0.0 0.0 0.0 6.700255045208296E-5 0.0 18-19 0.0 0.0 0.0 6.700255045208296E-5 0.0 20-21 0.0 0.0 0.0 1.340051009041659E-4 0.0 22-23 0.0 0.0 0.0 3.8526466509947703E-4 1.675063761302074E-5 24-25 0.0 0.0 0.0 7.370280549729125E-4 3.350127522604148E-5 26-27 0.0 0.0 0.0 0.0011725446329114517 3.350127522604148E-5 28-29 3.350127522604148E-5 0.0 0.0 0.004003402389511957 3.350127522604148E-5 30-31 3.350127522604148E-5 0.0 0.0 0.012395471833635348 5.025191283906222E-5 32-33 3.350127522604148E-5 0.0 0.0 0.028760844781556612 6.700255045208296E-5 34-35 3.350127522604148E-5 0.0 0.0 0.054339068416639276 6.700255045208296E-5 36-37 3.350127522604148E-5 0.0 0.0 0.09676843349042082 6.700255045208296E-5 38-39 6.700255045208296E-5 0.0 3.350127522604148E-5 0.15792501141555954 6.700255045208296E-5 40-41 8.375318806510369E-5 0.0 3.350127522604148E-5 0.22517882143183782 6.700255045208296E-5 42-43 1.0050382567812443E-4 0.0 3.350127522604148E-5 0.2928346467508286 6.700255045208296E-5 44-45 1.0050382567812443E-4 0.0 3.350127522604148E-5 0.3788491708936901 6.700255045208296E-5 46-47 1.0050382567812443E-4 0.0 3.350127522604148E-5 0.4855674831262452 6.700255045208296E-5 48-49 1.0050382567812443E-4 0.0 3.350127522604148E-5 0.6027716945045514 6.700255045208296E-5 50-51 1.0050382567812443E-4 0.0 3.350127522604148E-5 0.7250513490796027 6.700255045208296E-5 52-53 1.0050382567812443E-4 0.0 3.350127522604148E-5 0.8461417083841296 6.700255045208296E-5 54-55 1.0050382567812443E-4 0.0 3.350127522604148E-5 0.9864617996684044 6.700255045208296E-5 56-57 1.0050382567812443E-4 0.0 3.350127522604148E-5 1.1527956311657004 6.700255045208296E-5 58-59 1.0050382567812443E-4 0.0 3.350127522604148E-5 1.3418768285414786 6.700255045208296E-5 60-61 1.0050382567812443E-4 0.0 3.350127522604148E-5 1.5461508542322664 6.700255045208296E-5 62-63 1.0050382567812443E-4 0.0 3.350127522604148E-5 1.748766566799365 6.700255045208296E-5 64-65 1.0050382567812443E-4 0.0 3.350127522604148E-5 1.9571947506181824 6.700255045208296E-5 66-67 1.0050382567812443E-4 0.0 3.350127522604148E-5 2.200799273424343 6.700255045208296E-5 68-69 1.0050382567812443E-4 0.0 3.350127522604148E-5 2.4831815223046467 6.700255045208296E-5 70-71 1.0050382567812443E-4 0.0 3.350127522604148E-5 2.797490486475368 6.700255045208296E-5 72-73 1.0050382567812443E-4 0.0 3.350127522604148E-5 3.1065565010732135 6.700255045208296E-5 74-75 1.1725446329114518E-4 0.0 3.350127522604148E-5 3.414835235703247 6.700255045208296E-5 76-77 1.340051009041659E-4 0.0 3.350127522604148E-5 3.7544376626696296 6.700255045208296E-5 78-79 1.340051009041659E-4 0.0 3.350127522604148E-5 4.144224999924623 6.700255045208296E-5 80-81 1.675063761302074E-4 0.0 3.350127522604148E-5 4.5746326333911895 6.700255045208296E-5 82-83 1.675063761302074E-4 0.0 3.350127522604148E-5 5.006765582531899 6.700255045208296E-5 84-85 1.675063761302074E-4 0.0 3.350127522604148E-5 5.439451302713838 6.700255045208296E-5 86-87 1.675063761302074E-4 0.0 3.350127522604148E-5 5.893376831389087 6.700255045208296E-5 88-89 1.675063761302074E-4 0.0 3.350127522604148E-5 6.37926257662998 6.700255045208296E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10955 0.0 30.374607 1 GTATCAA 15730 0.0 28.687595 1 GTACATG 13765 0.0 24.862623 1 TACATGG 14020 0.0 23.95722 2 TATCAAC 19055 0.0 23.543947 2 ATCAACG 18935 0.0 23.543041 3 TCAACGC 18935 0.0 23.543041 4 ACATGGG 13960 0.0 23.211803 3 CAACGCA 19185 0.0 23.187254 5 AACGCAG 19610 0.0 22.886028 6 TACCTGG 2295 0.0 21.880709 2 GAGTACT 10145 0.0 21.841234 12-13 CTATACG 155 3.9901264E-5 21.394619 4 ACGCAGA 21790 0.0 20.359434 7 CGCAGAG 21985 0.0 20.070503 8 GTACTTT 11020 0.0 19.913845 14-15 CATGGGA 7545 0.0 19.527174 4 AGTACTT 10470 0.0 19.241383 12-13 CATGGGG 6510 0.0 19.138725 4 GCAGAGT 22970 0.0 18.736404 9 >>END_MODULE