FastQCFastQC Report
Thu 26 May 2016
SRR938703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938703_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984961
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113010.3785979113294948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99370.3329021719211742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63860.2139391435935009No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114800.028.9640851
GTATCAA161600.028.0340271
GTACATG138650.023.3290021
ATCAACG194050.023.2067933
TCAACGC195250.023.0641654
TACCTGG22500.022.9882852
TATCAAC197050.022.9498062
CAACGCA199150.022.6363225
TACATGG142250.022.6172622
AACGCAG202900.022.2647326
ACATGGG139850.022.191053
GAGTACT108900.020.82941812-13
ACGCAGA226050.019.9006127
CGCAGAG228250.019.7087978
GTACTTT115050.019.63348814-15
AGTACTT114450.019.48763712-13
GCAGAGT237950.018.745839
CATGGGG65900.018.6499184
GTACCTG29500.018.0859931
CATGGGA77150.018.0831474
Produced by FastQC (version 0.11.5)